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beta-lactamases producing anaerobic bacteria in dentoalveolar abscesses.pus samples of 30 patients with closed dentoalveolar abscesses who had not received antimicrobial therapy for at last two months were screened for the presence of beta-lactamase-producing anaerobic bacteria. from these 30 pus samples, a total of 112 bacterial strains were isolated; 83 of them were strict anaerobes and 29 were aerobes. beta-lactamases activity of the selected anaerobic bacteria was tested after identification of the isolates and was detected in 5 of the total 83 (6%) strict anaer ...199910693296
[anaerobic infection and its rapid detection in surgical patients].to investigate anaerobic infection and its rapid detection in surgical patients.199911829950
oral microbial flora in sjögren's syndrome.to study presence of microorganisms associated with caries, gingivitis, oral opportunistic infections, and extent of hyposalivation in dentate patients with primary and secondary sjögren's syndrome (ss).19999918250
microbiota of successful osseointegrated dental implants.the long-term survival of dental implants depends, in part, on control of bacterial infection in the peri-implant region. periodontal pathogens colonized implants symptomatic through infection, whereas the microbiota of successful implants was similar to that of periodontal health. this study examined the impact on the peri-implant microbiota of crown restorations; implant type; length of time of loading; history of implant or periodontal infections; and whether implants replaced single or multi ...199910102550
identification of positively charged residues of foma porin of fusobacterium nucleatum which are important for pore function.foma porin is the major outer-membrane protein of fusobacterium nucleatum. it exhibits the functional properties of a general diffusion porin, but has no sequence similarity to other porins. according to the proposed topology model, each monomer of this trimeric protein is a beta-barrel consisting of 16 transmembrane segments with eight surface-exposed loops. several conserved charged residues are proposed to extend from the beta-barrel wall into the aqueous channel lumen, and may contribute to ...199910103012
haemagglutination and haemolysis by oral fusobacterium nucleatum.haemagglutination and haemolytic activity of 80 fusobacterium nucleatum isolates from human and animal origin, on different human blood types was evaluated. all the isolates were able to agglutinate erythrocytes and the most were either alpha-haemolytic or beta-haemolytic. no specificity between haemolysin or haemagglutinin and blood type was observed. haemagglutination activity was inhibited when d-galactose, d-lactose or d-raffinose were used. haemagglutination and haemolysis may be important ...199910190119
coaggregation of candida dubliniensis with fusobacterium nucleatum.the binding of microorganisms to each other and oral surfaces contributes to the progression of microbial infections in the oral cavity. candida dubliniensis, a newly characterized species, has been identified in human immunodeficiency virus-seropositive patients and other immunocompromised individuals. c. dubliniensis phenotypically resembles candida albicans in many respects yet can be identified and differentiated as a unique candida species by phenotypic and genetic profiles. the purpose of ...199910203506
induction of anti-thymocyte/t lymphocyte antibodies in mice injected with lipopolysaccharides from periodontopathic bacteria.we examined the levels of anti-thymocyte/t lymphocyte autoantibody (ata) in the serum of mice injected intraperitoneally with lipopolysaccharides (lps) from periodontopathic bacteria; porphyromonas gingivalis, actinobacillus actinomycetemcomitans, fusobacterium nucleatum, capnocytophaga ochracea, and non-oral escherichia coli. all of the lps induced igm-ata. among these, lps from c. ochracea induced the highest level of igm-ata, whereas that of p. gingivalis induced the lowest. the peritoneal t ...199910207839
beta-lactamase production and antimicrobial susceptibility of oral heterogeneous fusobacterium nucleatum populations in young children.oral fusobacterium nucleatum populations from 20 young, healthy children were examined for beta-lactamase production. ten children (50%) harbored, altogether, 25 beta-lactamase-positive f. nucleatum isolates that were identified as f. nucleatum subsp. polymorphum, f. nucleatum subsp. nucleatum, and f. nucleatum subsp. vincentii (j. l. dzink, m. t. sheenan, and s. s. socransky, int. j. syst. bacteriol. 40:74-78, 1990). in vitro susceptibility of these beta-lactamase-producing and 26 non-beta-lact ...199910223950
periodontal bacteria in adult twins.both environmental and genetic factors are known to influence clinical measures of periodontal disease. the purpose of this study was to determine whether genetic factors similarly influence the presence of specific periodontal bacteria in subgingival plaque.199910225542
microbiological and clinical effects of a 1% chlorhexidine-gel in untreated periodontal pockets from adult periodontitis patients.the aim of this study was to report the microbiological and clinical effects of repeated subgingival administration of a 1% chlorhexidine-gel in periodontal pockets from 10 patients with adult periodontitis. results showed that the experimental treatment significantly improved clinical parameters (plaque index, gingival bleeding index, and pocket probing depth). direct subgingival administration of chlorhexidine-gel also produced a remarkable modification in the proportions of putative periodont ...199910322610
relationship of the subgingival microbiota to a chairside test for aspartate aminotransferase in gingival crevicular fluid.the aim of the present study was to evaluate the association between the occurrence of certain specific periodontal pathogens and aspartate aminotransferase (ast) levels in gingival crevicular fluid (gcf).199910052771
dominant cross-reactive antibodies generated during the response to a variety of oral bacterial species detect phosphorylcholine.the intraperitoneal immunization of balb/c mice with subgingival plaque from advanced periodontal pockets or with certain strains of actinobacillus actinomycetemcomitans, fusobacterium nucleatum, actinomyces israelii, streptococcus mitis, or streptococcus oralis yielded frequently indistinguishable igm monoclonal antibodies which were reactive with antigens from a variety of oral bacteria. this study aimed to characterize the specificity of such monoclonal antibodies and the diversity of oral ba ...199910065949
immune response to fusobacterium nucleatum and prevotella intermedia in patients with chronic maxillary sinusitis.our objective was to study the immune response to 2 anaerobic bacteria in patients with chronic maxillary sinusitis. immunoglobulin g class antibody titers to fusobacterium nucleatum and prevotella intermedia were measured by enzyme-linked immunosorbent assay. serum levels in the patients were determined on the day sinus aspiration for culture was done, and 75 to 90 days later, after completion of antimicrobial therapy, prevotella intermedia was isolated from 16 aspirates, and f. nucleatum from ...199910086624
unexpected cross-reaction with fusobacterium necrophorum in a pcr for detection of mycoplasmas.cross-reactions with fusobacterium necrophorum were found in a pcr designed for detection of a wide range of mycoplasma species. twenty-five strains of fusobacterium were examined; all 14 f. necrophorum strains reacted positively, whereas all 7 fusobacterium nucleatum strains reacted negatively. two strains that were not f. necrophorum yielded variable results.19999986867
elastase activity of anaerobes isolated from amniotic fluid with preterm premature rupture of membranes.a total of 131 anaerobes isolated from amniotic fluid with preterm premature rupture of membranes and stored were examined for elastolytic activity by the method described by williams et al (lett appl microbiol 1988;7:173-6).19999988804
abscess forming ability of streptococcus milleri group: synergistic effect with fusobacterium nucleatum.the abscess forming abilities of "streptococcus milleri" strains (streptococcus constellatus, streptococcus anginiosus, and streptococcus intermedius) isolated from dentoalveolar abscesses and the synergistic effect of fusobacterium nucleatum co-inoculated with the isolates were examined on a mouse subcutaneous abscess model. five days after inoculation, all s. milleri strains formed abscesses, which showed less pathological spread to surrounding connective tissues than those formed by staphyloc ...199910338189
effects of hydrogen peroxide on growth and selected properties of porphyromonas gingivalis.in this study we first evaluated the effects of hydrogen peroxide (h2o2) on growth and selected properties of porphyromonas gingivalis, and compared them with those obtained by a reducing agent (cysteine). the growth of p. gingivalis was only moderately affected when h2o2 was added at concentrations up to 30 mm in a complex culture medium. however, when a defined basal medium was used, h2o2 at a concentration of 3 mm completely inhibited growth of p. gingivalis. incorporation of cysteine at conc ...199910339828
linezolid activity compared to those of selected macrolides and other agents against aerobic and anaerobic pathogens isolated from soft tissue bite infections in humans.linezolid was tested against 420 aerobes and anaerobes, including 148 pasteurella isolates, by an agar dilution method. linezolid was active against all pasteurella multocida subsp. multocida and p. multocida subsp. septica isolates and most pasteurella canis, pasteurella dagmatis, and pasteurella stomatis isolates. the mic was </=2 microg/ml for staphylococci, streptococci, ef-4b, weeksella zoohelcum, fusobacterium nucleatum, other fusobacteria, porphyromonas spp., prevotella spp., peptostrepto ...199910348773
the effects of histatin-derived basic antimicrobial peptides on oral biofilms.susceptibility of bacteria to antimicrobial agents is strongly reduced by the formation of complex biofilms. we investigated whether synthetic histatin analogs with broad-spectrum antibacterial activity in vitro were also active against these complex mixtures of bacteria, as present in saliva and plaque. in a simplified model system for dental plaque, hydroxyapatite discs were placed in a continuous culture system comprised of streptococcus mutans, s. sanguis, s. salivarius, actinomyces naeslund ...199910371248
antibiotic susceptibility of putative periodontal pathogens in advanced periodontitis patients.in the present study, the antibiotic susceptibility of most prevalent micro-organisms in advanced periodontitis patients was evaluated. in 56 patients, pooled subgingival plaque samples were taken from the deepest site of each quadrant and were cultivated anaerobically. from each patient, the 4 most frequently encountered types of bacterial colonies were subcultured and identified (rapid id 32 a). from all bacterial species identified in the 224 subcultures, the 4 most prevalent were used for su ...199910382573
intra-familial distribution of fusobacterium nucleatum strains in healthy families with optimal plaque control.fusobacterium nucleatum, a gram-negative anaerobic rod associated with periodontal disease, is also found in healthy individuals and is considered part of the indigenous oral microflora. although intra-familial transmission of periodontal pathogens has been documented, there are no data relating transmission of f. nucleatum. this study investigated the distribution of f. nucleatum strains in 4 strictly healthy families. 32 f. nucleatum strains were isolated from 19 individuals (8 parents and 11 ...199910382581
flow cytometry to monitor phagocytosis and oxidative burst of anaerobic periodontopathogenic bacteria by human polymorphonuclear leukocytes.the reduced susceptibility to phagocytosis found among some periodontopathogenic anaerobes may account for the differences between invasive and non-invasive strains. we applied flow cytometry as a powerful tool to analyze and quantify phagocytosis using standardized cultures of oral anaerobes (porphyromonas gingivalis, prevotella intermedia, p. nigrescens, capnocytophaga gingivalis, c. ochracea, c. sputigena, fusobacterium nucleatum and peptostreptococcus micros) and heparinized whole blood. bac ...199910384401
beta-lactamase production by oral anaerobic gram-negative species in infants in relation to previous antimicrobial therapy.the frequency of beta-lactamase production in gram-negative bacteria has increased considerably during recent years. in this study, beta-lactamase production by oral anaerobic gram-negative rods isolated from saliva was longitudinally examined for 44 caucasian infants at the ages of 2, 6, and 12 months in relation to their documented exposure to antibiotics. isolates showing decreased susceptibility to penicillin g (1 microg/ml) were examined for beta-lactamase production by using a chromogenic ...199910390208
a 15-month evaluation of the effects of repeated subgingival minocycline in chronic adult periodontitis.a double-blind, randomized, parallel, comparative study was designed to evaluate the long-term safety and efficacy of subgingivally administered minocycline ointment versus a vehicle control.199910397521
clinical and microbiological effects of initial periodontal therapy in conjunction with amoxicillin and clavulanic acid in patients with adult periodontitis. a randomised double-blind, placebo-controlled study.the aim of the present study was to investigate the clinical and microbiological effects of initial periodontal therapy in conjunction with systemic amoxicillin plus clavulanic acid in adult periodontitis patients using a double-blind, parallel-group, and placebo-controlled protocol. 21 patients with a clinical diagnosis of generalised adult periodontitis were recruited. clinical measurements and microbiological assessments were carried out at baseline, 3, and 12 months post-treatment. approxima ...199910412851
comparative antimicrobial activity of an essential oil and an amine fluoride/stannous fluoride mouthrinse in vitro.although laboratory studies are not necessarily predictive of clinical activity; they can help to elucidate mechanisms underlying clinical activity when the latter has been established. in a recent clinical study, an essential oil mouthrinse (listerine antiseptic) was shown to be significantly more effective than an amine fluoride/stannous fluoride mouthrinse (meridol) in inhibiting supragingival plaque formation. this paper reports the results of laboratory studies comparing the antimicrobial e ...199910412853
porphyromonas gingivalis prevalence related to other micro-organisms in adult refractory periodontitis.forty-six adult periodontal patients, selected on the basis of clinical examination, and 46 adult healthy subjects were examined. the subgingival plaque samples from one inflammatory and one non-inflammatory site of each periodontal patient were studied to determine porphyromonas gingivalis prevalence related to other periodontal micro-organisms and to periodontal tissue destruction. the results showed porphyromonas gingivalis as the main pathogenic micro-organism isolated in the inflammatory si ...199910423739
oral microflora in subjects with reduced salivary secretion.it is generally assumed that a decreased salivary secretion rate will promote plaque accumulation and increase the risk for caries, gingival inflammation, and mucosal infections. in this study, the effect of hyposalivation on the oral microflora was examined. the following micro-organisms were analyzed in rinsing samples from 14 subjects with hyposalivation: the total number of anaerobically growing micro-organisms, alpha-hemolytic streptococci, mutans streptococci, lactobacilli, fusobacterium n ...199910439028
antigen activation of thp-1 human monocytic cells after stimulation with lipopolysaccharide from oral microorganisms and granulocyte-macrophage colony-stimulating factor.a human thp-1 monocyte cell line culture system has been utilized to evaluate the morphological changes in thp-1 cells and to measure expression of activation antigens (cd-11b, cd-11c, cd-14, cd-35, cd-68, cd-71 and hla-dr) as evidence of maturation of thp-1 cells in response to stimulation by lipopolysaccharide (lps) from the oral microorganisms, fusobacterium nucleatum and porphyromonas gingivalis, and granulocyte-macrophage colony-stimulating factor. thp-1 cells were stimulated with lps (1 mi ...199910444744
infection-stimulated infraosseus inflammation and bone destruction is increased in p-/e-selectin knockout mice.infections of the dental pulp commonly result in infraosseus inflammation and bone destruction. however, the role of phagocytic leucocytes in the pathogenesis of pulpal infections has been uncertain. in this work we used p/e-/- selectin-deficient mice, which lack rolling adhesion of leucocytes to endothelium and mimic the human syndrome, leucocyte adhesion deficiency ii (lad-ii), to test the hypothesis that phagocytic leucocytes protect against pulpal infection and subsequent periapical infraoss ...199910447722
fusobacterium nucleatum septicemia and portal vein thrombosis. 199910451179
the effects of hiv viral load on the phagocytic activity of monocytes activated with lipopolysaccharide from oral microorganisms.a study was undertaken to determine whether viral load status in hiv+ patients has any potential effect on monocyte phagocytic function both before and after challenge of the monocytes with lipopolysaccharide (lps) isolated from oral microorganisms. lps of two putative periodontal pathogens porphyromonas gingivalis (p. gingivalis) and fusobacterium nucleatum (f. nucleatum) was prepared. whole blood samples in edta were collected from 30 hiv+ patients presenting for dental care at the university ...199910466072
in vitro activity of gemifloxacin (sb 265805) against anaerobes.gemifloxacin mesylate (sb 265805), a new fluoronaphthyridone, was tested against 359 recent clinical anaerobic isolates by the national committee for clinical laboratory standards reference agar dilution method with supplemented brucella blood agar and an inoculum of 10(5) cfu/spot. comparative antimicrobials tested included trovafloxacin, levofloxacin, grepafloxacin, sparfloxacin, sitafloxacin (du-6859a), penicillin g, amoxicillin clavulanate, imipenem, cefoxitin, clindamycin, and metronidazole ...199910471570
microbiological response of localized sites with recurrent periodontitis in maintenance patients treated with tetracycline fibers.whether adjunctive tetracycline fibers can provide an additive effect to scaling and root planing in treating non-responsive sites in maintenance subjects is still controversial. recolonization of the bacteria from untreated sites or from the extracrevicular region may explain the insignificant response to local therapy. the purpose of the present study was to evaluate the microbiological response of sites treated with tetracycline fibers combined with scaling and root planing.199910476893
primary psoas abscess due to fusobacterium nucleatum.a case of primary pyogenic psoas abscess due to fusobacterium nucleatum is described. clinicians must maintain a high index of clinical suspicion for the diagnosis of psoas abscess. although staphylococcus aureus accounts for most cases of primary psoas abscess, this report emphasizes the importance of bacteriological confirmation of the microorganism involved.199910482067
microbiological features of papillon-lefèvre syndrome periodontitis.papillon-lefevre syndrome patients exhibit hyperkeratosis palmo-plantaris and severe periodontitis. the syndrome is an autosomal recessive trait, but the mechanism of periodontal destruction is not known. this report presents the clinical and microbiological features of an 11-year old girl with papillon-lefèvre syndrome. clinical examination included conventional periodontal measurements and radiographic analysis. in samples from 3 deep periodontal lesions, the occurrence of major suspected peri ...199910487314
2-hydroxyglutaryl-coa dehydratase from clostridium symbiosum.component d (hgdab) of 2-hydroxyglutaryl-coa dehydratase from clostridium symbiosum was purified to homogeneity. it is able to use component a from acidaminococcus fermentans (hgdc) to initiate catalysis together with atp, mg2+ and a strong reducing agent such as ti(iii)citrate. component d from c. symbiosum has a 6 x higher specific activity compared with that from a. fermentans and contains a second [4fe-4s] cluster but the same amount of riboflavin 5'-phosphate (1.0 per heterodimeric enzyme, ...199910491198
establishment of oral anaerobes during the first year of life.anaerobic species constitute a significant part of the bacterial community of the mouth. although the time and species involved in the primary colonization of infants are of great importance by forming the basis for further colonization, the development of the oral anaerobic microflora with age is still inadequately understood. in the present study, time and succession of colonization of oral anaerobes were longitudinally examined in 44 healthy caucasian infants at 2, 6, and 12 months of age. un ...199910520968
utilization of d-amino acids by fusobacterium nucleatum and fusobacterium varium.the utilization of d- and l-amino acids with acidic, basic or polar side chains was demonstrated by hplc. two species of the anaerobe fusobacterium utilized d-lysine and the l isomers of glutamate, glutamine, histidine, lysine and serine. only f. varium used l-arginine, d-glutamate and d-serine as substrates, whereas f. nucleatum specifically utilized d-histidine and d-glutamine. d-glutamate accumulated in f. nucleatum cultures supplemented with d-glutamine, and ornithine was detected when eithe ...199910524276
an evaluation of the action of different root canal irrigants on facultative aerobic-anaerobic, obligate anaerobic, and microaerophilic bacteria.the aim of this study was to test the effect of different concentrations of sodium hypochlorite, chlorhexidine, and cetrimide on the following bacterial strains: facultative aerobes-anaerobes: candida albicans atcc 10231; enterococcus faecalis atcc 29212; escherichia coli atcc 25,922; pseudomonas aeruginosa atcc 27,853; streptococcus mitis atcc 9811; streptococcus mutans atcc 35668; streptococcus salivarius atcc 13419; and streptococcus sanguis atcc 10556. microaerophiles: actinobacillus actinom ...199910530260
dexamethasone differentially regulates cytokine transcription and translation in macrophages responding to bacteria or okadaic acid.many microorganisms and microbial products induce expression of pro-inflammatory cytokines such as interleukin-1 (il-1alpha/beta) and tumour necrosis factor-alpha (tnf-alpha) in macrophages, primarily by transcriptional activation. we show here, by using mouse macrophages in primary culture, that pre-treatment with dexamethasone inhibits bacteria-induced il-1beta expression as mrna and cellular pro-il-1beta in parallel, consistent with an effect primarily on transcriptional activation. in contra ...199910530874
arbitrarily primed-polymerase chain reaction for identification and epidemiologic subtyping of oral isolates of fusobacterium nucleatum.fusobacterium nucleatum is the most frequently isolated bacterium in periodontal disease and plays an important role in serious infections in other parts of the body. arbitrarily primed-polymerase chain reaction (ap-pcr) was used to construct primers for specific identification and subtyping of f. nucleatum. subtypes may differ in virulence and, hence, are important as periodontal pathogens. subtypes also may differ in antibiotic susceptibility; therefore, knowing the subtypes may influence choi ...199910534075
infective endocarditis due to fusobacterium nucleatum in an intravenous drug abuser.infective endocarditis due to anaerobic non-spore-forming gram-negative bacilli in intravenous drug abusers is exceedingly rare, with only two cases being previously reported in the literature. a case of endocarditis due to fusobacterium nucleatum in an intravenous drug abuser is reported, accompanied by a review of the literature.199910534188
enhanced interleukin-8 production in thp-1 human monocytic cells by lipopolysaccharide from oral microorganisms and granulocyte-macrophage colony-stimulating factor.granulocyte-macrophage colony-stimulating factor (gm-csf) has been used to assist in bone marrow recovery during cancer chemotherapy. interleukin-8 (il-8) plays an important role in macrophage mediated inflammatory processes including exacerbation of periodontal diseases, one of the most common complications in gm-csf receiving cancer patients. the effect of gm-csf supplementation on il-8 production was investigated in a human monocyte cell line thp-1, stimulated with lipopolysaccharide extracte ...199910551153
nosocomial sinusitis with isolation of anaerobic bacteria in icu patients.to determine the frequency and the eventual clinical characteristics of nosocomial sinusitis with anaerobic bacteria isolation in patients in the intensive care unit (icu).199910551960
acquisition of plasmin activity by fusobacterium nucleatum subsp. nucleatum and potential contribution to tissue destruction during periodontitis.fusobacterium nucleatum subsp. nucleatum has been associated with a variety of oral and nonoral infections such as periodontitis, pericarditis, bone infections, and brain abscesses. several studies have shown the role of plasmin, a plasma serine protease, in increasing the invasive capacity of microorganisms. in this study, we investigated the binding of human plasminogen to f. nucleatum subsp. nucleatum, and its subsequent activation into plasmin. plasminogen-binding activity of bacterial cells ...199910569761
fusobacterium nucleatum empyema necessitans. 199910585835
longitudinal study of dental implants in a periodontally compromised population.the purpose of this longitudinal study was to determine the clinical status and the composition of the subgingival microbiota of dental implants and natural teeth in patients with a history of periodontitis.199910588495
pre- and post-implantation microbiota of the tongue, teeth, and newly placed implants.this investigation sought intra-oral sources of species colonizing dental implants.199910599911
periodontopathic bacteria in english hiv-seropositive persons.selected periodontopathic bacteria were sought in 20 hiv-infected english patients and eight noninfected control subjects with similar periodontal status, using highly specific dna probes. actinobacillus actinomycetemcomitans (a. a), porphyromonas gingivalis, campylobacter rectus, prevotella intermedia, and fusobacterium nucleatum were more frequently detected and were found at higher levels in hiv-infected individuals than in controls. significantly increased levels of treponema denticola but, ...199910842858
prevalence of beta-lactamase-producing strains among 149 anaerobic gram-negative rods isolated from periodontal pockets.in a prospective study, 47 adults presenting a rapidly progressive periodontitis were selected in order to evaluate the prevalence of beta-lactamase-producing strains among oral anaerobic gram-negative rods. predominant anaerobes were identified from two of the deepest periodontal pockets. beta-lactamase-positive strains fulfilled to at least two of three criteria: positive nitrocefin test, penicillin etest minimal inhibitory concentration > 1 microgram/ml, and disk diffusion synergy between amo ...199910895690
comparing periodontal disease in identical twins: a case report.previous investigators have shown that numerous environmental and genetic variables may contribute to the pathogenesis of periodontal disease. this case report presents clinical and laboratory findings of a set of caucasian female identical twins. one patient presented clinically with mild gingivitis and no clinical or radiographic signs of periodontitis. the other exhibited gingivitis with localized, moderate-to-severe periodontitis. neither patient reported a history of systemic conditions tha ...199910634119
in vitro volatile sulfur compound production of oral bacteria in different culture media.the purpose of this study was to detect the relative contribution of porphyromonas gingivalis, fusobacterium nucleatum, and prevotella intermedia in the production of oral malodor.199910635291
inflammatory cytokine production and specific antibody responses to lipopolysaccharide from endodontopathic black-pigmented bacteria in patients with multilesional periapical periodontitis.we examined the induction of the cytokines interleukin (il)-1 beta, il-6, and il-8 by lipopolysaccharides (lpss) from several species of possible endodontopathic black-pigmented bacteria. studies were conducted in human whole blood cultures from six patients (two from each group) with differing numbers of periapical periodontitis lesions (i.e. patients with radiographically clear periapical lesions in 10 or more teeth (high-lesion group, n = 4), in one or two teeth (low-lesion group, n = 6), and ...199910726523
development of a clindamycin-impregnated fiber as an intracanal medication in endodontic therapy.the effectiveness of traditional endodontic intracanal medications in reducing bacterial numbers and preventing acute flare-ups and pain continues to be questioned. in the present study, a new local delivery device was developed that releases a substantive dose of clindamycin into root canals. clindamycin-impregnated ethylene vinyl acetate (eva) fibers were produced, and the sensitivity of common endodontic microbes to the fibers were established. an in vitro model was developed to persistently ...199910726537
streptococcus viridans intra-amniotic infection associated with antecedent cunnilingus.introduction of bacteria into the amniotic cavity has been associated with oral sex through an ascending infectious route. previous reports have implicated both capnocytophaga and fusobacterium nucleatum via this process. we report a case of streptococcus viridans intra-amniotic infection occurring at 25 weeks' gestation. patient questioning revealed a close temporal relationship between the onset of symptoms and previous episodes of cunnilingus. the diagnosis of subclinical bacterial colonizati ...199910755795
bacterial colonisation during gtr treatment. a longitudinal analysis.the purpose of the present study was to evaluate the long-term biological and clinical effects of a controlled delivery system releasing tetracyclines during the healing of interproximal periodontal defects treated by the technique of guided tissue regeneration (gtr) using e-ptfe membranes.199910768009
inducible expression of human beta-defensin 2 by fusobacterium nucleatum in oral epithelial cells: multiple signaling pathways and role of commensal bacteria in innate immunity and the epithelial barrier.human gingival epithelial cells (hge) express two antimicrobial peptides of the beta-defensin family, human beta-defensin 1 (hbd-1) and hbd-2, as well as cytokines and chemokines that contribute to innate immunity. in the present study, the expression and transcriptional regulation of hbd-2 was examined. hbd-2 mrna was induced by cell wall extract of fusobacterium nucleatum, an oral commensal microorganism, but not by that of porphyromonas gingivalis, a periodontal pathogen. hbd-2 mrna was also ...200010768988
profile of subgingival microbiota in children with primary dentition.eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. the aim of the study was to investigate the composition of the subgingival microbiota of primary incisors, canines and molars in 40 systemically healthy children aged 4-5 yr, chosen randomly. subgingival plaque samples were taken from the mesiobuccal sites of primary incisors (61, 81), canines (53, 73) and molars (64, 84). the samples were cultured for bacterial isolat ...200010791707
the prevalence of pathogenic periodontal microflora in healthy young adult smokers.smoking is a major risk factor in periodontitis, although the mechanisms of its effects are not well understood. the overall goal of this clinical study was to determine if smoking enhances the colonization of the oral cavity by pathogenic bacteria in a periodontitis-free population. the prevalence of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, campylobacter rectus, eikenella corrodens, bacteroides forsythus, and treponema denti ...200010807119
interactions between periodontal bacteria and human oral epithelial cells: fusobacterium nucleatum adheres to and invades epithelial cells.bacteria are causative agents of periodontal diseases. interactions between oral bacteria and gingival epithelial cells are essential aspects of periodontal infections. using an in vitro tissue culture model, a selected group of gram-negative anaerobic bacteria frequently associated with periodontal diseases, including bacteroides forsythus, campylobacter curvus, eikenella corrodens, fusobacterium nucleatum, porphyromonas gingivalis, and prevotella intermedia, were examined for their ability to ...200010816455
review of the in vitro activity of gemifloxacin against gram-positive and gram-negative anaerobic pathogens.published reports on the in vitro activity of gemifloxacin mesylate (sb 265805), a new fluoronaphthyridone, against anaerobic pathogens are reviewed here. the studies used a variety of media, inocula and antimicrobial agents. using a proposed breakpoint of 0.5 mg/l, these studies showed that gemifloxacin had generally higher potency against gram-positive anaerobes (clostridium perfringens, all peptostreptococcus spp.) and fusobacteria (fusobacterium nucleatum, fusobacterium necrophorum) and mode ...200010824034
the response to oxidative stress of fusobacterium nucleatum grown in continuous culture.fusobacterium nucleatum atcc 10953 was grown in continuous culture and the atmosphere changed stepwise from nitrogen (anaerobiosis) to a mixture of air: oxygen (40:60). no significant differences in biomass were observed and the baseline low level of superoxide dismutase increased only slightly; catalase and peroxidase activities were never detected but the level of nadh oxidase increased more than three-fold when oxygen was introduced into the system. in relation to acidic end-products, the rel ...200010828396
retrospective identification and characterization of candida dubliniensis isolates among candida albicans clinical laboratory isolates from human immunodeficiency virus (hiv)-infected and non-hiv-infected individuals.fungal opportunistic infections, and in particular those caused by the various candida species, have gained considerable significance as a cause of morbidity and, often, mortality. the newly described species candida dubliniensis phenotypically resembles candida albicans so closely that it is easily misidentified as such. the present study was designed to determine the frequency at which this new species is not recognized in the clinical laboratory, to determine the patient populations with whic ...200010835022
development of oral bacterial flora in young children.the intimate relationship with bacteria is a fundamental factor in the health status of an individual. after birth infants are exposed to continuous person-to-person and environmental contacts with microbes, and the development of the indigenous microflora begins on the surfaces of the human body. in a developing ecosystem microbial colonization may easily occur because of the still inadequate host response. adhesion is the initial event in the colonization of bacteria. in the mouth, only mucosa ...200010766401
native plasmids of fusobacterium nucleatum: characterization and use in development of genetic systems.three native plasmids of fusobacterium nucleatum were characterized, including dna sequence analysis of one plasmid, pfn1. a shuttle plasmid, phs17, capable of transforming escherichia coli and f. nucleatum atcc 10953 was constructed with pfn1. phs17 was stably maintained in the f. nucleatum transformants, and differences in the transformation efficiencies suggested the presence of a restriction-modification system in f. nucleatum.200010648549
the effect of scaling and root planing on the clinical and microbiological parameters of periodontal diseases: 12-month results.previously, we reported that srp resulted in a decrease in mean pocket depth and attachment level and reduced prevalence and levels of bacteroidesforsythus, porphyromonas gingivalis, and treponema denticola at 3 and 6 months post-srp in 57 subjects with adult periodontitis. 32 of the 57 subjects were monitored at 9 and 12 months. thus, the purpose of the present investigation was to evaluate the microbial and clinical effects of srp in 32 (mean age 48+/-11) subjects over a 12-month period.200010674959
isolation and characterization of a human neutrophil aggregation defective mutant of fusobacterium nucleatum.fusobacterium nucleatum is known to adhere to human polymorphonuclear neutrophils (pmns) and cause them to aggregate. in this study, we isolated a spontaneously occurring aggregation defective (agg(-)) mutant and this mutant will be used for future study of the interactions between this bacterium and human pmn. genomic dna fingerprinting by random-primed polymerase chain reaction method revealed a difference between the parent strain and the agg(-) mutant. this mutant also showed an altered phen ...200010683469
in vitro activities of mk-826 (l-749,345) against 363 strains of anaerobic bacteria.the activity of mk-826 was compared to the activities of cefoxitin, ceftriaxone, imipenem, and meropenem against 363 gram-negative and gram-positive anaerobes by using nccls procedures. at least 98% of the strains were susceptible to the carbapenems. all strains of clostridium perfringens, fusobacterium nucleatum, peptostreptococcus, and sutterella wadsworthensis were susceptible to all agents tested.200010898709
toll-like receptor 4-deficient mice have reduced bone destruction following mixed anaerobic infection.c3h/hej mice have an impaired ability to respond to lipopolysaccharide (lps) due to a mutation in the gene that encodes toll-like receptor 4 (tlr4). the effect of tlr4 deficiency on host responses to endodontic infections is unknown. in the present study, we compared periapical bone destruction, sepsis, and inflammatory cytokine production in lps-hyporesponsive c3h/hej and wild-type control c3h/heouj mice. the mandibular first molars of both strains were subjected to pulpal exposure and infectio ...200010899873
interleukin-1 receptor signaling rather than that of tumor necrosis factor is critical in protecting the host from the severe consequences of a polymicrobe anaerobic infection.infection of the dental pulp leads to an osteolytic lesion that results from a polymicrobial infection consisting largely of pathogenic anaerobes. infection causes significant morbidity and mortality mediated by bacterial factors and in some cases by the up-regulation of inflammatory cytokines. the inflammatory cytokines interleukin-1 (il-1) and tumor necrosis factor (tnf), in particular, play a complex and central role in the responses to microbial pathogens. however, relatively little is known ...200010899881
characterization of bacterial orofacial infections using a new murine model.we devised a new murine orofacial infection model using bacteria from odontogenic infection origins and characterized the experimental infections. in this model, bacteria were injected into the submandible of mice. streptococcus constellatus and peptostreptococcus micros produced a single abscess at the injection site and their abscess-forming and lethal abilities were low: the median abscess-forming dose (af(50)) of s. constellatus and p. micros were 10(8.5-10.7)and 10(10.2-10.6)cfu/mouse, and ...200010906266
fusobacterial infections: an underestimated threat.the involvement of fusobacteria in a wide range of human and animal infection has long been recognised. slow-growing anaerobes, often in polymicrobial culture, they are not always identified but are present mainly in the oropharynx, from where they are bloodborne to other sites or aspirated into the lung. fusobacterium nucleatum is commonly found in periodontal disease and produces tissue irritants such as butyric acid, proteases and cytokines. it has strong adhesive properties due to the presen ...200010912293
"checkerboard" assessments of periodontal microbiota and serum antibody responses: a case-control study.we explored the association between subgingival microbial profiles and serum igg responses to periodontal microbiota in relation to clinical periodontal status.200010914791
tetracycline fibres used to control bacterial infection during guided tissue regeneration (gtr).the colonization of suspected periodontal pathogens during the healing of periodontal defects treated by guided tissue regeneration (gtr) with e-ptfe membranes and tetracycline fibers was investigated.200010932905
enhanced interleukin-1beta, interleukin-6 and tumor necrosis factor-alpha production by lps stimulated human monocytes isolated from hiv+ patients.periodontal disease and tooth loss is a common finding among advanced hiv+ patients. in addition to local oral lipopolysaccharide (lps) stimulation, systemic up-regulation of monocyte pro-inflammatory cytokine secretion may also be involved in the pathogenesis of hiv disease. a study was undertaken to investigate il-1beta, il-6 and tnf-alpha production by resting and lps stimulated monocytes isolated from hiv+ patients and also to investigate the relationship of the patient's hiv viral load stat ...200010946822
comparative in vitro activities of abt-773 against aerobic and anaerobic pathogens isolated from skin and soft-tissue animal and human bite wound infections.we studied the comparative in vitro activities of abt-773, a new ketolide, against 268 aerobic and 148 anaerobic recent isolates from clinical bites using an agar dilution method and inocula of 10(4) cfu/spot for aerobes and 10(5) cfu for anaerobes. the following are the mic ranges and mics at which 90% of isolates are inhibited (mic(90)s) of abt-773 for various isolates, respectively: pasteurella multocida and pasteurella septica, 0.125 to 2 and 1 microg/ml; other pasteurella species, 0.125 to ...200010952607
fusobacterium nucleatum involvement in adult periodontitis and possible modification of strain classification.this investigation was designed to evaluate the involvement of fusobacterium nucleatum clinical strains in adult periodontitis by subspecies and expression of hemagglutination activity.200010960022
identification of periodontal disease-associated bacteria in the "plaque-free zone".subgingival plaque bacteria live within a biofilm covered with glycocalyx, and little is known of the bacterial species associated with biofilm formation at the bottom of human periodontal pockets, the so-called "plaque-free zone"(pfz).200010972648
laboratory diagnosis of anaerobic pleuropulmonary infections.anaerobic organisms play a major role in pleuropulmonary infections. clinical manifestations range from simple aspiration to acute, severe, necrotizing pneumonias to chronic infections, such as lung abscess and empyema. implicated pathogens are usually of endogenous origin. laboratory diagnosis of anaerobic pleuropulmonary infections is based on recovering the etiological agent from clinical specimens. appropriate specimens include pleuml fluid, transtracheal aspirates, transthoracic aspirates a ...200010983929
b-cell deficiency predisposes mice to disseminating anaerobic infections: protection by passive antibody transfer.we have previously demonstrated that a high proportion of rag-2 scid knockout mice, which lack t and b cells, develop orofacial abscesses and disseminated infections following pulpal infection, whereas immunocompetent control mice do not. in the present study, we sought to identify the components of the adaptive immune response which contribute to protection against disseminating anaerobic infections and sepsis. for this purpose, various genetically engineered immunodeficient mice were employed, ...200010992465
[analysis using elisa test of antibody response to fusobacterium nucleatum and eikenella corrodens in subjects with periodontal disease].to know the synthesis of igg, iga, and igm to fusobacterium nucleatum and eikenella corrodens in serum, crevicular liquid and saliva in subjects with periodontal disease, using elisa test.200010996873
the fusobacterium nucleatum porin foma possesses the general topology of the non-specific porins.foma is a major non-specific porin of fusobacterium nucleatum with no sequence similarity to other known porins. according to the topology model, the protein consists of 16 transmembrane beta-strands, connected by eight surface-exposed loops and seven periplasmic turns. in this study, the insertion mutagenesis approach was applied to probe the topology model. a semliki forest virus (sfv) epitope was successfully inserted at 11 different sites of the foma protein and a 6-aa insertion was successf ...200010846222
identification of candida dubliniensis in a study of hiv-seropositive pediatric dental patients.the combination of an immature immune system and suppressed cellular immunity in children with hiv infections provides optimal conditions for rapid disease progression. as a result, pediatric aids has become a major epidemiological challenge. oral fungal colonization remains one of the most common opportunistic infections observed in both adult and pediatric hiv infected patients. although candida albicans is the most frequently isolated opportunistic fungal species, a recently characterized can ...200010846737
in vitro activity of rifaximin, metronidazole and vancomycin against clostridium difficile and the rate of selection of spontaneously resistant mutants against representative anaerobic and aerobic bacteria, including ammonia-producing species.rifaximin is a rifamycin derivative characterized by a wide antibacterial activity. this drug is neither absorbed by the gastrointestinal tract nor inactivated by gastric juices, and exerts its action entirely within the intestinal lumen.200010859431
lipopolysaccharide induces expression of genes encoding pro-inflammatory cytokines and the elastin-degrading enzyme, cathepsin s, in human cervical smooth-muscle cells.vaginal and amniotic infection with gram-negative bacteria is associated with preterm birth. we previously reported that human cervical smooth-muscle cells (csmc) respond to pro-inflammatory cytokines by expressing enzymes that degrade the extracellular matrix. our objective was to characterize the effects of lipopolysaccharide (lps) from escherichia coli (e coli), bacteroides fragilis, (b frag)and fusobacterium nucleatum (f nuc)on the expression of pro-inflammatory cytokines and the elastin-deg ...200010865188
effects of streptococcus crista and human saliva on the viability of fusobacterium nucleatum atcc 10953.associations with facultative species might help planktonic oral anaerobes survive when they traverse saliva. this study investigated whether co-aggregation with streptococcus crista atcc 51110 enhanced the viability of fusobacterium nucleatum atcc 10953. two tubes each of co-aggregates and fus. nucleatum atcc 10953 alone were prepared. one of each set was resuspended in buffer, and the other in clarified saliva from each of 20 donors. after 1 h, cells were stained for viability. the median perc ...200010869478
influence of antimicrobial subinhibitory concentrations on hemolytic activity and bacteriocin-like substances in oral fusobacterium nucleatum.fusobacterium nucleatum is considered for its role in colonization of initial and late microorganisms in dental plaque and for its coaggregation with other bacterial species. it is known that action of different antimicrobial substances may interfere with either virulence factors or with host-bacteria interaction. the goal of this study was to examine the influence of subinhibitory concentrations of chlorhexidine, triclosan, penicillin g and metronidazole on hemolytic activity and bacteriocin-li ...200010872683
features of severe periodontal disease in a teenager with chédiak-higashi syndrome.chédiak-higashi syndrome (c-hs) is a rare congenital disease characterized by defective neutrophil function with abnormal lysosomal inclusions, neutropenia, and reduced chemotaxis. the complete syndrome includes oculocutaneous albinism with photophobia, neurologic features, recurrent infections, and enterocolitis.200010872965
the periodontal abscess (i). clinical and microbiological findings.little information is available regarding the diagnosis and microbiology of periodontal abscesses. the aim of this descriptive clinical and microbiological study was to provide more information in order to help in the characterisation of the periodontal abscess associated to periodontitis.200010883867
peptostreptococcus micros coaggregates with fusobacterium nucleatum and non-encapsulated porphyromonas gingivalis.coaggregation is one of the potential colonization strategies of oral microorganisms, often involving fimbrial structures in the interactions. in this study, the coaggregation characteristics of the rough and smooth genotypes of the periodontal pathogen peptostreptococcus micros were compared to investigate the role of the fibril-like structures of the rough genotype in coaggregation. of the 11 oral species tested, only fusobacterium nucleatum strains and non-encapsulated porphyromonas gingivali ...200010612731
[effect and mechanism of garlic juice and hydrogen peroxide on the degradation of lipopolysaccharide].to elucidate the effect and mechanism of garlic juice and hydrogen peroxide on the degradation of lipopolysaccharide (lps).200011780236
isolation of fusobacteria from the oral cavities of malnourished nigerian children living in agricultural and herding villages.a previous study demonstrated the presence and possible involvement of fusobacterium necrophorum in the pathogenesis of noma lesions of children living in agricultural and herding villages in northwestern nigeria. in order to determine if f. necrophorum was part of the oral flora of malnourished children with no noma lesions, a study of the fusobacteria present in the oral cavities of 30 children, 2-6 years of age in sokoto state, was undertaken. swabs taken of the oral cavity were cultured on s ...200010702787
induction of apoptotic cell death in peripheral blood mononuclear and polymorphonuclear cells by an oral bacterium, fusobacterium nucleatum.it is largely unknown why a variety of bacteria present in the oral cavity are capable of establishing themselves in the periodontal pockets of nonimmunocompromised individuals in the presence of competent immune effector cells. in this paper we present evidence for the immunosuppressive role of fusobacterium nucleatum, a gram-negative oral bacterium which plays an important role in the generation of periodontal disease. our studies indicate that the immunosuppressive role of f. nucleatum is lar ...200010722579
[the neutralizing effect of histidine-rich-polypeptides on lps from suspected periodontal pathogens].human parotid histidine-rich-polypeptides (hrps) are a family of lowmolecular-weight, cationic polypeptides. hrp-1, hrp-3 and hrp-5 comprise 85%-90% of the total hrps and are called major hrps. there were many researches indicating the antimicrobial activities of hrps. recently, hrps were reported to have an inhibitory action on the lipopolysaccharide (lps) of e. coli, with hrp-5 being the most effective one among the three major hrps. since the lps of oral gram-negative bacteria is thought to b ...200012539645
detection of major putative periodontopathogens in korean advanced adult periodontitis patients using a nucleic acid-based approach.although extensive microbial analyses have been performed from subgingival plaque samples of periodontitis patients, systematic analysis of subgingival microbiota has not been carried out in a korean population so far. the purpose of this study was to describe the prevalence of major putative periodontopathogens in korean patients by culture-independent methods.200011022767
cytokine, elastase and oxygen radical release by fusobacterium nucleatum-activated leukocytes: a possible pathogenic factor in periodontitis.periodontitis is characterised by tissue destruction caused by reactive oxygen species (ros) and proteolytic enzymes, which are released by the interaction between bacteria and phagocytes. we estimated the ability of fusobacterium species to induce release of tissue destructive and proinflammatory mediators from in vitro activated peripheral leukocytes. ros was measured with the nitroblue tetrazolium (nbt) method, elastase with a specific chromogenic substrate and cytokines, including interleuki ...200011034124
differences in the composition of the subgingival microbiota of two periodontitis populations of different geographical origin. a comparison between spain and the netherlands.the purpose of this study was to compare the subgingival microbiota of two geographically distinct patient populations using identical clinical and bacteriological methods. adult patients with a diagnosis of periodontitis were consecutively selected according to pre-defined clinical criteria. microbiological samples were taken from the deepest four sites with bleeding. the samples were plated on blood agar plates, for the determination of the total anaerobic counts and identification of specific ...200011037754
automated immunofluorescence for enumeration of selected taxa in supragingival dental plaque.the present study investigated a recently developed automated image analysis technique for its applicability to the enumeration of selected bacteria in supragingival dental plaque. following initial calibration, the system is capable to count fluorescence-labeled target cells in up to 48 samples without user interference. test samples contained a characteristic mixture of planktonic bacteria, small almost planar bacterial aggregates, and large, virtually indisruptable clumps with cells from mult ...200011037755
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