Publications

structure, viability and bacterial kinetics of an in vitro biofilm model using six bacteria from the subgingival microbiota.	there are few in vitro models available in the scientific literature for study of the structure, formation and development of the subgingival biofilm. the purpose of this study was to develop and validate an in vitro biofilm model, using representative selected bacteria from the subgingival microbiota.	2011	21261622
antigenicity of primary endodontic infection against macrophages by the levels of pge(2) production.	root canal contents are potent stimuli for proinflammatory cytokines involved in apical periodontitis. this study investigated target gram-negative bacterial species and endotoxins in primary endodontic infection with apical periodontitis, determined their antigenicity against macrophages through the levels of pge(2), and evaluated their relationship with clinical findings.	2011	21496656
the effect of xylitol on the composition of the oral flora: a pilot study.	our aim was to investigate the effect of short-term xylitol consumption on the microbial composition of plaque and saliva.	2011	21311610
the origin of fusobacterium nucleatum involved in intra-amniotic infection and preterm birth.	objective. to evaluate the potential oral origin of fusobacterium nucleatum found in amniotic fluid of women at high risk of preterm birth. methods. a transversal study nested into a cohort study of women with preterm labor and/or preterm premature rupture of membranes was undergone. women with the presence of f. nucleatum in the amniotic fluid and their respective partners were invited to be examined for their periodontal health after delivery, and samples of saliva and subgingival plaque were ...	2011	21314291
the role of acidification in the inhibition of neisseria gonorrhoeae by vaginal lactobacilli during anaerobic growth.	vaginal lactobacilli protect the female genital tract by producing lactic acid, bacteriocins, hydrogen peroxide or a local immune response. in bacterial vaginosis, normal lactobacilli are replaced by an anaerobic flora and this may increase susceptibility to neisseria gonorrhoeae, a facultative anaerobe. bacterial interference between vaginal lactobacilli and n. gonorrhoeae has not been studied in liquid medium under anaerobic conditions. by co-cultivating n. gonorrhoeae in the presence of lacto ...	2011	21329492
inhibitory effect of lactobacillus reuteri on periodontopathic and cariogenic bacteria.	the interaction between lactobacillus reuteri, a probiotic bacterium, and oral pathogenic bacteria have not been studied adequately. this study examined the effects of l. reuteri on the proliferation of periodontopathic bacteria including aggregatibacter actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gingivalis, and tannerella forsythia, and on the formation of streptococcus mutans biofilms. human-derived l. reuteri strains (kctc 3594 and kctc 3678) and rat-derived l. reuteri kctc ...	2011	21538238
short- and medium-chain fatty acids exhibit antimicrobial activity for oral microorganisms.	this study assessed the antibacterial activity of short-, medium-, and long-chain fatty acids against various oral microorganisms.	2011	21333271
association between periodontal pathogens and risk of nonfatal myocardial infarction.	the direct effect of periodontal pathogens on atherosclerotic plaque development has been suggested as a potential mechanism for the observed association between periodontal disease and coronary heart disease, but few studies have tested this theory.	2011	21375559
rna-oligonucleotide quantification technique (roqt) for the enumeration of uncultivated bacterial species in subgingival biofilms.	approximately 35% of the species present in subgingival biofilms are as yet uncultivated, so their role in periodontal pathogenesis is unknown. the aim of the present study was to develop a high throughput method to quantify a wide range of cultivated and uncultivated taxa in subgingival biofilm samples associated with periodontal disease or health. oligonucleotides targeting the 16s ribosomal dna gene were designed, synthesized and labeled with digoxigenin. these probes were hybridized with the ...	2011	21375703
streptococcus cristatus attenuates fusobacterium nucleatum-induced cytokine expression by influencing pathways converging on nuclear factor-?b.	we previously reported that streptococcus cristatus, an oral commensal, was able to downregulate the interleukin-8 (il-8) response to fusobacterium nucleatum, a putative oral pathogen in oral epithelial cells. the aim of this study was to extend the understanding of how s. cristatus regulates cytokine expression in oral epithelial cells on a broad basis, and investigate whether the modulation of a toll-like receptor (tlr) pathway was involved in this process. kb and tert-2 cells were co-cultured ...	2011	21375705
in-vivo-induced antigenic determinants of fusobacterium nucleatum subsp. nucleatum.	fusobacterium nucleatum plays a pivotal role in dental plaque biofilm formation and is known to be involved in chronic inflammatory systemic disease. however, limited knowledge of f. nucleatum genes expressed in vivo interferes with our understanding of pathogenesis. in this study, we identified f. nucleatum genes induced in vivo using in-vivo-induced antigen technology (iviat). among 30,000 recombinant clones screened, 87 reacted reproducibly with pooled sera from 10 patients with periodontitis ...	2011	21375706
fusobacterium nucleatum in periodontal health and disease.	the pathogenesis of periodontitis involves the interplay of microbiota present in the subgingival plaque and the host responses. inflammation and destruction of periodontal tissues are considered to result from the response of a susceptible host to a microbial biofilm containing gram-negative pathogens. antimicrobial peptides are important contributors to maintaining the balance between health and disease in this complex environment. these include several salivary antimicrobial peptides such as ...	2011	21220789
mpc-polymer reduces adherence and biofilm formation by oral bacteria.	oral biofilms such as dental plaque cause dental caries and periodontitis, as well as aspiration pneumonia and infectious endocarditis by translocation. hence, the suppression of oral biofilm formation is an issue of considerable importance. mechanical removal, disinfectants, inhibition of polysaccharide formation, and artificial sugar have been used for the reduction of oral biofilm. from the viewpoint of the inhibition of bacterial adherence, we investigated whether aqueous biocompatible 2-met ...	2011	21447697
invasive potential of gut mucosa-derived fusobacterium nucleatum positively correlates with ibd status of the host.	background:: fusobacterium nucleatum is a heterogeneous oral pathogen that is also a common resident of the human gut mucosa. given that some strains of f. nucleatum are known to be invasive and proinflammatory in the oral mucosa, we compared strains isolated from patients with inflammatory bowel disease (ibd) with strains isolated from healthy controls to determine 1) whether this species was more commonly associated with ibd patients; and 2) whether gut-derived f. nucleatum strains from ibd pa ...	2011	21234912
clinical spectrum of bacteraemic fusobacterium infections: from septic shock to nosocomial bacteraemia.	fusobacterium species are anaerobic bacteria that relatively rarely cause sepsis with a variable clinical presentation.	2011	21391770
clinical periodontal and microbiologic parameters in patients with rheumatoid arthritis.	background: a limited number of studies suggest the prevalence of periodontal pathogens in patients with rheumatoid arthritis (ra); however, results are inconsistent. the aim of this study was to investigate clinical periodontal and microbiologic parameters in patients with ra. methods: 66 patients with ra aged 49.5±8.4 years participated in the study. the periodontal situation was assessed with the periodontal screening index (psr(®)/psi); allocated to: healthy, gingivitis (psr(®)/psi score 0-2 ...	2011	21405936
clustering of subgingival microbial species in adolescents with periodontitis.	it has become increasingly recognized that groups of microorganisms interact within the subgingival plaque of adult subjects with periodontitis. it is much less clear, however, whether the consortia of microorganisms associated with periodontitis are different in early and more advanced cases of periodontitis. to investigate this point further, subgingival plaque was collected from six sites in 87 adolescents with periodontitis and 73 controls and the samples were analyzed for the detection of 1 ...	2011	21410554
structural basis of cooperative ligand binding by the glycine riboswitch.	the glycine riboswitch regulates gene expression through the cooperative recognition of its amino acid ligand by a tandem pair of aptamers. a 3.6 +à crystal structure of the tandem riboswitch from the glycine permease operon of fusobacterium nucleatum reveals the glycine binding sites and an extensive network of interactions, largely mediated by asymmetric a-minor contacts, that serve to communicate ligand binding status between the aptamers. these interactions provide a structural basis for how ...	2011	21439473
detection of periodontal bacteria in atheromatous plaques by nested polymerase chain reaction.	background. in recent years, increasing evidence regarding the potential association between periodontal diseases and cardiovascular diseases has been elicited. the available evidence underlines the importance of detecting periodontal pathogens on atheromatous plaques as the first step in demonstrating the causal relationship between both entities. it is the main aim of this investigation to detect periodontitis-associated bacteria from carotid artery atheromatous plaques recovered from patients ...	2011	21453047
the oral commensal, streptococcus gordonii, synergizes with tat protein to induce hiv-1 promoter activation in monocytes/macrophages.	trans-activator of transcription (tat) is an hiv-1 protein essential for viral replication. oral periodontopathogens (e.g. fusobacterium nucleatum) enhance hiv-1ltr promoter activation in monocytes/macrophages in absence of tat; however, some oral commensals fail to trigger this response. we sought to determine the effect of tat on hiv-1ltr promoter activation induced by the representative oral commensal streptococcus gordonii in monocytes/macrophages. s. gordonii enhanced hiv-1ltr reactivation ...	2011	21459369
multispecies communities: interspecies interactions influence growth on saliva as sole nutritional source.	human oral bacteria live in multispecies communities in the biofilm called dental plaque. this review focuses on the interactions of seven species and the ability of each species individually and together with other species to grow on saliva as the sole source of nutrient. community formation in biofilms in flow cells is monitored using species-specific fluorophore-conjugated immunoglobulin g, and images are captured by confocal microscopy. early colonizing veillonellae emerge from this review o ...	2011	21485308
analysis of interspecies adherence of oral bacteria using a membrane binding assay coupled with polymerase chain reaction-denaturing gradient gel electrophoresis profiling.	information on co-adherence of different oral bacterial species is important for understanding interspecies interactions within oral microbial community. current knowledge on this topic is heavily based on pariwise coaggregation of known, cultivable species. in this study, we employed a membrane binding assay coupled with polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) to systematically analyze the co-adherence profiles of oral bacterial species, and achieved a more ...	2011	21485313
microbial shifts during dental biofilm re-development in the absence of oral hygiene in periodontal health and disease.	uzel ng, teles fr, teles rp, song xq, torresyap g, socransky ss, haffajee ad. microbial shifts during dental biofilm re-development in the absence of oral hygiene in periodontal health and disease. j clin peridontol 2011; doi: 10.1111/j.1600-051x.2011.01730.x. abstract: aim: to monitor microbial shifts during dental biofilm re-development. materials and methods: supra- and subgingival plaque samples were taken separately from 28 teeth in 38 healthy and 17 periodontitis subjects at baseline and i ...	2011	21488936
influence of topography and hydrophilicity on initial oral biofilm formation on microstructured titanium surfaces in vitro.	objectives: the aim of this study was to analyse the influence of the microtopography and hydrophilicity of titanium (ti) substrates on initial oral biofilm formation. materials and methods: nine bacterial species belonging to the normal oral microbiota, including: aggregatibacter actinomycetemcomitans, actinomyces israelii, campylobacter rectus, eikenella corrodens, fusobacterium nucleatum, parvimonas micra, porphyromonas gingivalis, prevotella intermedia, and streptococcus sanguinis were teste ...	2011	21492236
identification and enzymatic analysis of a novel protein associated with production of hydrogen sulfide and l-serine from l-cysteine in fusobacterium nucleatum subsp. nucleatum atcc 25586.	a third enzyme that produced hydrogen sulfide from l-cysteine was identified in fusobacterium nucleatum subsp. nucleatum. the fn1055 gene was cloned from a cosmid library constructed with the genomic dna of f. nucleatum atcc 25586. despite the database annotation that the product of fn1055 is a cysteine synthase, reverse phase hplc revealed that no l-cysteine was produced in vitro by the purified fn1055 protein; however, the enzyme did produce l-serine. in addition, a cysteine auxotroph, escheri ...	2011	21493682
streptococcus cristatus modulates the fusobacterium nucleatum-induced epithelial interleukin-8 response through the nuclear factor-kappa b pathway.	zhang g, chen r, rudney jd.streptococcus cristatusmodulates thefusobacterium nucleatum-induced epithelial interleukin-8 response through the nuclear factor-kappa b pathway. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01373.x.©2011 john wiley & sons a/s background and objective: we previously reported that the interleukin-8 (il-8) response to fusobacterum nucleatum was attenuated in the presence of streptococcus cristatus. here, we further examined the underlying mechanism(s) involved in ...	2011	21521225
Antibacterial activity of sphingoid bases and fatty acids against gram-positive bacteria and gram-negative bacteria.	There is growing evidence that the role of lipids in innate immunity is more important than previously realized. How lipids interact with bacteria to achieve a level of protection, however, is still poorly understood. To begin to address mechanisms of antibacterial activity, we determined minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of lipids common to the skin and oral cavity: sphingoid bases D-sphingosine, phytosphingosine, and dihydrosphingosine, and f ...	2011	22155833
spiramycin resistance in human periodontitis microbiota.	purpose: the occurrence of in vitro resistance to therapeutic concentrations of spiramycin, amoxicillin, and metronidazole was determined for putative periodontal pathogens isolated in the united states. materials and methods: subgingival plaque specimens from 37 consecutive adults with untreated severe periodontitis were anaerobically cultured, and isolated putative periodontal pathogens were identified to a species level. in vitro resistance to spiramycin at 4 μg/ml, amoxicillin at 8 μg/ml, an ...	2011	21524712
fusobacterium nucleatum infection of colonic cells stimulates muc2 mucin and tumor necrosis factor-{alpha}	even though the etiology of inflammatory bowel disease is not completely known, it is influenced by the presence of normal gut microflora as well as-yet, unrecognized pathogens. the anaerobic, gram-negative bacterial species, fusobacterium nucleatum, is a common resident of the human mouth and gut and varies in its pathogenic potential. in this study, we demonstrate that highly invasive f. nucleatum isolates derived from the inflamed gut of crohn's disease patients evoked significantly greater m ...	2011	21536792
interaction of oral bacteria with gingival epithelial cell multilayers.	primary gingival epithelial cells were cultured in multilayers as a model for the study of interactions with oral bacteria associated with health and periodontal disease. multilayers maintained at an air-liquid interface in low-calcium medium displayed differentiation and cytokeratin properties characteristic of junctional epithelium. multilayers were infected with fluorescently labeled porphyromonas gingivalis, aggregatibacter actinomycetemcomitans, fusobacterium nucleatum or streptococcus gord ...	2011	21545698
antibacterial compounds from siraitia grosvenorii leaves.	luo han guo (lhg) fruits (siraitia grosvenorii swingle) have been used as traditional medicine in china for centuries to treat sore throats and coughs. however, lhg leaves are seldom used and minimal scientific information is available on them. in our recent study on the leaves of s. grosvenorii, the bioactive compounds β-amyrin (2), aloe emodin (5), aloe-emodin acetate (6), 5α,8α-epidioxy-24(r)-methylcholesta-6,22-dien-3β-ol (7) and p-hydroxyl benzyl acid (8), accompanied by n-hexadecaoic acid ...	2011	21547839
garlic allicin as a potential agent for controlling oral pathogens.	abstract garlic has been used medicinally throughout human history. allicin is considered the most therapeutic constituent of garlic. this study tested the antimicrobial activity of garlic allicin on oral pathogens associated with dental caries and periodontitis. allicin was found effective against all the tested bacteria. the broth dilution method revealed that planktonic growth of the cariogenic, gram-positive species streptococcus mutans, s. sobrinus, and actinomyces oris was inhibited by an ...	2011	21548800
oxidative stress response in the opportunistic oral pathogen fusobacterium nucleatum.	the anaerobic, gram-negative bacillus fusobacterium nucleatum plays a vital role in oral biofilm formation and the development of periodontal disease. the organism plays a central bridging role between early and late colonizers within dental plaque and plays a protective role against reactive oxygen species. using a two-dimensional gel electrophoresis and mass spectrometry approach, we have annotated 78 proteins within the proteome of f. nucleatum subsp. nucleatum and identified those proteins w ...	2011	21563313
antimicrobial effect of adjunctive use of chlorhexidine mouthrinse in untreated gingivitis: a randomized, placebo-controlled study.	becerik s, türkoğlu o, emingil g, vural c, özdemir g, atilla g. anti-microbial effect of adjunctive use of chlorhexidine mouthrinse in untreated gingivitis: a randomized, placebo-controlled study. apmis 2011; 119: 364-72. the aim of this study was to examine the effectiveness of chlorhexidine mouthrinse (chx) in addition to daily plaque control on subgingival microbiota in patients with untreated gingivitis. fifty gingivitis patients were randomized to chx or placebo groups. chx group rinsed wit ...	2011	21569094
characteristics and outcomes of fusobacterium nucleatum bacteremia-a 6-year experience at a tertiary care hospital in northern taiwan.	fusobacterium nucleatum bacteremia is critical and not well defined. to identify the clinical characteristics and outcomes, we conducted a retrospective review of hospitalized patients from january 2004 to december 2009 at a tertiary center in northern taiwan. fifty-seven patients were enrolled. the mean age was 58.1 years, and the mean pitt bacteremia score was 4.7. males predominated (59.6%), and the overall 30-day mortality rate was up to 47.4%. malignancy was the major comorbidity (26/57, 45 ...	2011	21596220
[weight loss and dyspnea in a 46-year-old woman.]		2011	21601965
acute appendicitis is characterised by local invasion with fusobacterium nucleatum/necrophorum.	acute appendicitis is a local intestinal inflammation with unclear origin. the aim was to test whether bacteria in appendicitis differ in composition to bacteria found in caecal biopsies from healthy and disease controls.	2011	19926616
antiaggregation potential of berry fractions against pairs of streptococcus mutans with fusobacterium nucleatum or actinomyces naeslundii.	coaggregation is an interspecies adhesion process, which is essential to the development of dental plaque. this is an in vitro study of the composition of the soluble solids in the berry juice molecular size fractions (<10 kda, fi; 10-100 kda, fii; >100 kda, fiii) derived from apple, bilberry, blackcurrant, cloudberry, crowberry and lingonberry and their ability to inhibit and reverse coaggregation of the pairs of common species in dental plaque: streptococcus mutans with fusobacterium nucleatum ...	2011	20623601
fusobacterium nucleatum regulation of neutrophil transcription.	abnormal neutrophil responses have been observed in periodontitis patients, including hyper-reactivity in terms of production of reactive oxygen species (ros) following exposure to the key quorum-sensing plaque bacterium, fusobacterium nucleatum. this study was designed to characterize the transcriptional response of neutrophils to f. nucleatum.	2011	20663022
the spectrum of gastroenteritis at the pediatric outpatient clinic, department of child health, school of medicine, university of north sumatera in 1982.		2011	3237422
learning from lesions: patterns of tissue inflammation in leprosy.	the clinical forms of leprosy constitute a spectrum that correlates closely with the degree of cell-mediated immunity. patients with tuberculoid leprosy develop strong cell-mediated responses and have only a few, localized lesions, whereas patients with multibacillary lepromatous leprosy are specifically unresponsive to antigens of myobacterium leprae. t cells of the cd4+ subset predominate in tuberculoid lesions, whereas cd8+ cells predominate in lepromatous lesions. monoclonal antibodies that ...	2011	3257577
learning from lesions: patterns of tissue inflammation in leprosy.	the clinical forms of leprosy constitute a spectrum that correlates closely with the degree of cell-mediated immunity. patients with tuberculoid leprosy develop strong cell-mediated responses and have only a few, localized lesions, whereas patients with multibacillary lepromatous leprosy are specifically unresponsive to antigens of myobacterium leprae. t cells of the cd4+ subset predominate in tuberculoid lesions, whereas cd8+ cells predominate in lepromatous lesions. monoclonal antibodies that ...	2011	3257577
differences in latencies and amplitudes of auditory brain stem responses in symmetrical areas of the scalp in healthy subjects.		2011	3201914
second stage in the war against asbestos is about to be unleashed.		2011	3173953
second stage in the war against asbestos is about to be unleashed.		2011	3173953
esthetic partial coverage restorations.		2011	3248246
[andrological abnormalities in internal diseases and following drug therapy].	in the present paper the following non-endocrine internal diseases are discussed: liver cirrhosis, diabetes, chronic renal failure and morbus crohn. in alcoholic liver patients under fifty, hypospermia and oligozoospermia can be observed. the hormone assays showed moderately increased fsh- and lh-values in the serum; prolactin, testosterone and estradiol remained normal. an increased binding of testosterone to shbg is supposed, and the androgen deficiency symptoms are considered to be due to the ...	2011	3115148
long ischaemic stricture of the interposed colon.	a case is reported of a long segment stricture in a colonic oesophageal replacement with radiographic evidence to support the diagnosis of ischaemia. ischaemia of the substitute organ following oesophageal resection is a well recognised, serious complication that usually results in anastomotic leakage. if the anastomosis heals primarily, late manifestations of ischaemia are rare.	2011	3206389
carcinoma of the penis as a metastasis.	a case of carcinoma of the penis is reported where the tumour was metastatic in origin. the patient recovered postoperatively. based on the histological study of the removed tumour, suspicion of bronchial cancer arose to be the primary tumour on the right side. subsequently, despite the physician's advice, the patient left the department of pulmonology for home and so no further pulmonological examination and treatment were made. one year postoperatively, local recurrence in the penis could not ...	2011	3239331
value of cerebrospinal fluid analysis in the differential diagnosis of meningitis: a study in 710 patients with suspected central nervous system infection.	a prospective study to determine the value of cerebrospinal fluid analysis in the differential diagnosis of meningitis was performed in 710 consecutively observed patients, both children and adults, who underwent lumbar puncture due to suspected central nervous system infection. diagnoses included acute or presumed bacterial meningitis (n = 79), acute or presumed viral meningoencephalitis (n = 218), acute unclassified meningitis (n = 6), other infections of the central nervous system (n = 37), n ...	2011	3137038
antiimmunoglobulin-treated b cells respond to a b cell differentiation factor for igg1.	we have determined whether b cells previously activated by anti-ig (anti-ig blasts) are responsive to lymphokines that induce isotype switching. culture of anti-ig blasts with a mixture of lymphokines, including bsf-1, resulted in marked secretion of igm and igg1, but not other igg isotypes. the igg1 response of anti-ig blasts to lymphokines was 13-fold greater than was observed with splenic b cells. b cell blasts induced by 8-mercaptoguanosine or dextran sulfate did not secrete high levels of a ...	2011	3088197
the national health service versus private and complementary medicine.		2011	3132291
[arthropathies of hemodialyzed patients].		2011	3206138
[hypergastrinemia and hypertrophic pyloric stenosis in infants].		2011	3173737
diacylglycerols, lysolecithin, or hydrocarbons markedly alter the bilayer to hexagonal phase transition temperature of phosphatidylethanolamines.	the bilayer to hexagonal phase transition temperatures of dielaidoylphosphatidylethanolamine and 1-palmitoyl-2-oleoylphosphatidylethanolamine are 65.6 and 71.4 degrees c, respectively. using high-sensitivity differential scanning calorimetry, i have shown that these transition temperatures are extremely sensitive to the presence of small amounts of other lipid components. for example, at a mole fraction of only 0.01, dilinolenin lowers the bilayer to hexagonal phase transition temperature of 1-p ...	2011	4084564
[operative procedures for right sleeve lobectomy].		2011	3225981
[a case of cystic changes in a giant leiomyoblastoma of the stomach].	a 44-year-old male was admitted to hospital with a complaint of abdominal distention. a ct scan revealed a giant multicystic mass in the abdominal cavity, a tumor that measured 34 cm in diameter, which had risen from the gastric body and grew in an exophytic fashion with a stalk. histologic findings exhibited a malignant leiomyoblastoma with cellular pleomorphism and a intracytoplasmic cystic change, which included disseminated nests of immature small round cells. after excisional surgery, the p ...	2011	3204665
hypoxaemia during recovery from anaesthesia--an audit of children after general anaesthesia for routine elective surgery.	arterial oxygen saturation was measured by pulse oximetry in 105 children (aged 2 weeks-14 years) during recovery from general anaesthesia. oxygen saturation was monitored continuously from the time that anaesthesia ended in the operating theatre until the children were fit to leave the recovery ward. all children breathed room air during transfer to the recovery area; 81 children continued to breathe room air in the recovery ward while the remainder received supplementary oxygen. fifty-six chil ...	2011	3213926
the national health service versus private and complementary medicine.		2011	3132291
[intracranial aneurysms and subarachnoid hemorrhage in children and adolescents].	42 cases concerning symptomatic intracranial circulation aneurysms and spontaneous sah have been observed by the authors during the period 1965-1984; 37 of them were treated from 1970 on. this group represents 2.6% in the number of patients treated for sah during the above mentioned period in the series of pavia and verona neurosurgery. in 7 cases angiography did not evidence any malformation responsible for the hemorrhage. these cases have been defined as "sine materia" sah. in 33 cases was doc ...	2011	3725140
igg subclass deficiency in asthma.	total immunoglobulin g (igg) and subclasses were measured in serum samples from 82 children with chronic asthma, aged 1.5 to 6.3 years, and 76 controls. concentrations of igg1, igg2, igg3, and total igg were significantly lower in asthmatic children aged 1 to 5, and igg2 concentrations were also significantly lower in asthmatic children over 5 years of age. twenty eight asthmatic children had at least one value in the deficient range, and 26 had igg2 deficiency alone or in combination. five chil ...	2011	3232989
gas chromatographic method for simultaneous analysis of urinary testosterone, androsterone and etiocholanolone. determinations after a single dose of testosterone.		2011	3237733
[skeletal changes following long-term treatment with retinoids].	the synthetic retinoids, the vitamin-d-derivatives etretinate and isotretinoin, have substantially enlarged the therapeutic arsenal in dermatology. they are primarily used in severe cases of acne and cornification disorders. in the majority of cases, long-term treatment is necessary. certain side effects in the skeletal system can occur, e.g., osteoporosis, premature epiphyseal closure, and changes similar to dish (diffuse idiopathic skeletal hyperostosis). we discuss the reports in the literatu ...	2011	3045876
ganglion cells in the turtle retina contain the neuropeptide lant-6.	this study investigated the presence of the neurotensin-related hexapeptide, lant-6, in retinal ganglion cells and their central projections in the turtle pseudemys scripta elegans. immunocytochemical techniques demonstrated that many of the cells in the ganglion cell layer of the turtle retina could be labeled with an antiserum specific for lant-6. radioimmunoassay and chromatographic analysis confirmed the presence of lant-6-related peptides in retina, as well as brain. several molecular forms ...	2011	3339403
antenatal diagnosis of neural tube defects using a coated bead immunoassay for acetylcholinesterase in amniotic fluid.	the development and validation of a coated bead immunoassay for amniotic fluid acetylcholinesterase is outlined. the assay has good precision (between assay cv of 6.8% within the normal range), and is linear up to 250 arbitrary units/l. the clinical validity of this assay has been assessed using a panel of amniotic fluid samples from normal and abnormal pregnancies. at an assay cut off level of 200 arbitrary units/l, all cases of neural tube defect-affected pregnancies were identified and the nu ...	2011	3232959
skin surface temperature over the masseter muscle in individuals with rheumatoid arthritis.	temperature measurements were made on the skin surface over the masseter muscle in 71 individuals with rheumatoid arthritis (ra group) and in 52 individuals without general joint disease or symptoms (c group). the temperature recordings were performed with thermistors in contact with the skin. symptoms in the stomatognathic system and general joint symptoms were investigated by means of a questionnaire. a clinical examination was made of the stomatognathic system. in addition, a medical examinat ...	2011	3165585
3-hydroxyoctanoic aciduria: identification of a new organic acid in the urine of a patient with non-ketotic hypoglycemia.	a four-month-old child with non-ketotic hypoglycemia and rapidly progressive cirrhosis excreted in her urine large amounts of two unidentified organic acids in addition to a spectrum of saturated, unsaturated, and 3-hydroxy dicarboxylic acids in her urine. gas chromatography/mass spectrometry of the trimethylsilyl derivative of one of the unknown compounds suggested the structure of 3-hydroxyoctanoic acid, which was confirmed by similar analysis of the authentic compound. the same organic acid w ...	2011	3168281
recognition and prevention of pseudothrombocytopenia and concomitant pseudoleukocytosis.	unrecognized anticoagulant-induced platelet (plt) aggregation, leading to pseudothrombocytopenia and concomitant pseudoleukocytosis, can have serious clinical consequences. it can be readily recognized by inspecting conventional blood smears or the white blood cell histograms generated by modern blood cell counters. blood specimens from twenty patients with known edta-induced platelet aggregation were consecutively drawn into three other anticoagulants (using vacutainer tubes) and processed in a ...	2011	3128946
[embryonal rhabdomyosarcoma diagnosed prenatally. apropos of a case].	a case report of congenital embryonal rhabdomyosarcoma of the superior extremity (right shoulder) is described. this patient was the first and only child of a 24 year-old woman who had been previously treated for sterility. the diagnosis was done by echography during the 36 th week of gestation. this full term white boy was born by cesarean section in reason of tumor size. first he was treated surgically. then, a cyclic chemotherapy (vac) was performed. after 4 courses, local recurrence led to a ...	2011	3228943
[embryonal rhabdomyosarcoma diagnosed prenatally. apropos of a case].	a case report of congenital embryonal rhabdomyosarcoma of the superior extremity (right shoulder) is described. this patient was the first and only child of a 24 year-old woman who had been previously treated for sterility. the diagnosis was done by echography during the 36 th week of gestation. this full term white boy was born by cesarean section in reason of tumor size. first he was treated surgically. then, a cyclic chemotherapy (vac) was performed. after 4 courses, local recurrence led to a ...	2011	3228943
uses and limitations of the balance technique.	classical balance techniques are a powerful, sensitive, and usually accurate technique for assessing the nutritional or metabolic response to changes in nutritional intake or to metabolic or physiological perturbations. balances are particularly sensitive for examining transient or short-term responses to nutritional or metabolic stimuli. a major factor responsible for the precision and sensitivity of this technique is the precise control of the activities and environment of an individual during ...	2011	3312697
[subchronic oral toxicity test of dicofol (1,1-bis(p-chlorophenyl)-2,2,2-trichloroethanol) as the basis for the design of a long-term carcinogenicity study in b6c3f1 mice].		2011	3449167
results of mechanical ventricular assist in bridging to cardiac transplantation.		2011	3277739
results of mechanical ventricular assist in bridging to cardiac transplantation.		2011	3277739
[immunologic indices in patients with cancer of the breast following surgical treatment].		2011	3210623
age and infertility.		2011	3105771
information induction for predicting acute myocardial infarction.	we show how to make an unsupervised discrimination of disease and nondisease states by measuring information and using newer notions of inductive reason. we also present a new theory of group-based reference values that is based on measuring information uncertainty. we use data on the isoenzymes creatine kinase-mb (ck-mb) and lactate dehydrogenase-1 (ld1) and on the percentage of ld1 from 101 patients with acute myocardial infarction (ami) and from 41 patients with suspected, but unfounded, infa ...	2011	3168215
[fibrous lesions of the skeleton in infants and children].		2011	3122625
age and infertility.		2011	3105771
opiate withdrawal unmasks tourette's syndrome.		2011	3233593
[congenital disorders of color perception are revealed in pattern electroretinography].		2011	3265690
[congenital disorders of color perception are revealed in pattern electroretinography].		2011	3265690
perforations of the tympanic membrane following the use of goode-type 'long term' tympanostomy tubes.	a retrospective review of 64 goode 't' tube insertions is presented. these were inserted into 61 ears of 48 patients over a period of four years. at the time of review of these 61 ears, 34 tubes were found to be in situ, 27 had extruded spontaneously and three had been removed surgically. ten ears in which the tube had been extruded or removed had central perforations of the tympanic membrane of which eight had been present for more than six months. the number of previous myringotomies or gromme ...	2011	3209952
identity--lost and found: patterns of migration and psychological and psychosocial adjustment of migrants.	this paper focuses on patterns of integration in migrant individuals and families. the level of adjustment in traditional and westernized families has been examined. different factors affecting adjustment are pointed out. migration process is interpreted as a transient and necessary crisis situation. risk factors in the acculturation crisis are reported and predictive and preventive measures are suggested. characteristics of migrant and remigrant groups are compared. the role of "homesickness" i ...	2011	3067526
an instructional module on testicular cancer for adolescent males.		2011	3152330
frequencies of complex diseases in hybrid populations.	diseases of complex etiology demonstrate considerable variation in their frequencies in different ethnic populations. noninsulin-dependent diabetes mellitus (niddm), rheumatoid arthritis, and several cardiovascular diseases constitute examples of such disorders. in genetic studies involving hybrid populations of known ancestry, it is of interest to compare and correlate disease prevalence with the admixture proportion, the latter estimated from a number of polymorphic genetic markers. theoretica ...	2011	3766715
[psychological preparation of children for surgery is also of postoperative importance].		2011	3200021
[clinical observations on a new preparation with hepatoprotective action].		2011	4234340
polymicrobial bacteremia in children. an 11-year experience.	the clinical records of all patients with blood cultures positive for a bacterial pathogen were retrospectively examined during an 11-year period to determine the rate of and clinical features associated with polymicrobial bacteremia. during this period, bacteria were isolated in 6302 blood cultures. of these cultures, 38 instances (0.6%) of polymicrobial bacteremia occurred in 38 patients. in 37 patients (97%), an underlying condition was identified that was considered a predisposing factor for ...	2011	3177321
informal networks of community-based elderly. changes in composition over time.		2011	3227153
discussion group summary: ethical dilemmas in the clinical setting--time constraints, conflicts in interprofessional decision making.		2011	3205685
[the importance of medical documentation in legal medicine expertise].		2011	3270203
[the importance of medical documentation in legal medicine expertise].		2011	3270203
frequencies of complex diseases in hybrid populations.	diseases of complex etiology demonstrate considerable variation in their frequencies in different ethnic populations. noninsulin-dependent diabetes mellitus (niddm), rheumatoid arthritis, and several cardiovascular diseases constitute examples of such disorders. in genetic studies involving hybrid populations of known ancestry, it is of interest to compare and correlate disease prevalence with the admixture proportion, the latter estimated from a number of polymorphic genetic markers. theoretica ...	2011	3766715
[arrival of the injured at the hospital].		2011	3358035
[arrival of the injured at the hospital].		2011	3358035
renal cell carcinoma presenting with gross hematuria from a solitary bladder metastasis.	renal cell carcinoma commonly presents with gross hematuria. we report the first case in which the diagnosis of renal cell carcinoma was made following biopsy of an actively bleeding solitary bladder metastasis of a clear cell carcinoma of the kidney. the possible routes of metastasis of this uncommon lesion are discussed and a review of the literature is presented.	2011	3215266
assessment of contractility changes in humans by transoesophageal echocardiography: the peak-systolic pressure end-systolic diameter relationship (pspesdrs).	the peak-systolic pressure end-systolic diameter relationship (pspesdrs) was determined in ten healthy subjects under general anaesthesia by using the radial artery pressure tracing (peak-systolic pressure) and transoesophageal 2d-and m-mode echocardiography (left ventricular diameters) without blocking of autonomic reflexes. left ventricular load was changed by injection of a single intravenous dose of 0.2 mg nitroglycerin (ntg). linear regression analysis showed a close linear relationship dur ...	2011	3119748
questionable role of amniocentesis in the etiology of amniotic band formation. a case report.	a preterm infant had an amniotic band sequence. anomalies included left leg amputation and digital anomalies of the hands. the umbilical cord was short, and histologic sections of the placenta demonstrated partial avulsion of the amnion and fibrosis of the underlying chorion. ultrasound at 21 weeks was normal, there was no history of trauma during the pregnancy, and no amniocentesis had been performed. despite isolated case reports of amniotic band sequence associated with amniocentesis, we doub ...	2011	3351810
[effect of kavinton on the hemodynamics and adenosine metabolism in atherosclerosis patients].		2011	3188457