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acid tolerance and acid-neutralizing activity of porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum.the tolerance to acid and the acid-neutralizing activity of three important periodontopathic bacteria, porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum were studied. p. gingivalis strains grew only at neutral ph and did not utilize glucose, whereas strains of p. intermedia and f. nucleatum could grow under acidic conditions and increased their growth by utilizing glucose. p. gingivalis tended to raise the culture ph during growth. p. intermedia and f. nucleatum raised ...19979573805
characterization of a novel n-acetylneuraminic acid-specific fusobacterium nucleatum pk1594 adhesin.fusobacterium nucleatum has been identified as significantly associated with sites with active periodontal disease and, as a group, the oral fusobacteria coaggregate with members of all oral bacteria genera tested. monoclonal antibodies were prepared and used in conjunction with other potential inhibitors, such as simple sugars and amino acids, to characterize coaggregation interactions, of f. nucleatum pk1594. four unique monoclonal antibodies, 5h11, 14c7, 19f2 and 29c12, were obtained by their ...19989573822
helicobacter pylori adheres selectively to fusobacterium spp.helicobacter pylori strains atcc 43504 and atcc 43629 were tested for their ability to coaggregate with 79 strains of bacteria representing 16 genera. all except two of the strains were of human origin, and most of the strains were isolated from the oral cavity. the helicobacters failed to coaggregate with all strains except the fusobacteria. several coaggregations were partially or completely inhibited by lactose. strong coaggregation was seen with each of four subspecies of fusobacterium nucle ...19989573823
trovafloxacin compared with levofloxacin, ofloxacin, ciprofloxacin, azithromycin and clarithromycin against unusual aerobic and anaerobic human and animal bite-wound pathogens.the activity of trovafloxacin and five other oral agents against 250 aerobic and 137 anaerobic strains isolated from human and animal bite wounds was determined by an agar dilution method. trovafloxacin was active against all aerobic and fastidious facultative isolates at < or = 0.5 mg/l and all anaerobes at < or = 2 mg/l (bacteroides tectum, porphyromonas salivosa and prevotella heparinolytica, < or = 0.25 mg/l; porphyromonas spp., < or = 0.5 mg/l; prevotella spp. and peptostreptococci, < or = ...19989578167
coronal leakage: endotoxin penetration from mixed bacterial communities through obturated, post-prepared root canals.coronal leakage has been considered as one of the major causes of failure in root canal treatment. bacteria have been shown to penetrate the root canal obturating materials and influence the periapical tissues. endotoxin, a component of gram-negative bacteria, is a potent inflammatory agent and may be able to penetrate obturating materials faster than bacteria. this in vitro study examined the possible penetration of post-prepared canals by endotoxin and bacterial cells derived from mixed bacter ...19989922745
interleukin-6 production by human monocytes treated with granulocyte-macrophage colony-stimulating factor in the presence of lipopolysaccharide of oral microorganisms.this study focused on the effect of granulocyte-macrophage colony-stimulating factor (gm-csf) and lipopolysaccharide of the putative periodontal pathogens porphyromonas gingivalis or fusobacterium nucleatum on il-6 production by thp-1 cells (a human monocytic cell line). resting thp-1 cells were alternatively treated with gm-csf (50 iu/ml) and lipopolysaccharide of p. gingivalis or f. nucleatum, in varying concentrations for varying time periods. il-6 production in supernatant fluids of treated ...199810093532
inflammatory cytokine production and specific antibody responses against possible causative bacteria in patients with multilesional periapical periodontitis.we examined the induction of inflammatory cytokines (including interleukin-1, interleukin-6, interleukin-8, and tumor necrosis factor-alpha) by several species of possible causative bacteria in periapical periodontitis. assays were done on human whole blood cultures from patients with differing numbers of periapical lesions; those having radiographically clear periapical lesions in 10 or more teeth (high lesion group), in one or two teeth (low lesion group), and healthy volunteers having no peri ...199810023261
the microbial spectrum of brain abscess with special reference to anaerobic bacteria.the bacteriological and clinical findings of 18 cases of brain abscess are presented. identification and antimicrobial susceptibility of anaerobic strains was performed both by conventional methods and the newer rapid ana ii panel and e-test methods, respectively. characterization of the anaerobic isolates was done by restriction fragment length polymorphism (rflp). anaerobic or aerobic organisms alone were recovered in three (16.6%), and mixed aerobic and anaerobic in another three (16.6%) pati ...199811013664
dentilisin activity affects the organization of the outer sheath of treponema denticola.prolyl-phenylalanine-specific serine protease (dentilisin) is a major extracellular protease produced by treponema denticola. the gene, prtp, coding for the protease was recently cloned and sequenced (k. ishihara, t. miura, h. k. kuramitsu, and k. okuda, infect. immun. 64:5178-5186, 1996). in order to determine the role of this protease in the physiology and virulence of t. denticola, a dentilisin-deficient mutant, k1, was constructed following electroporation with a prtp-inactivated dna fragmen ...19989683480
clonal analysis by ribotyping of fusobacterium nucleatum isolates obtained from healthy young adults with optimal plaque control.fusobacterium nucleatum is a gram-negative anaerobic rod implicated in the pathogenesis of periodontal disease. however, this organism has also been frequently identified in high numbers in healthy adults. these observations suggest that the species may comprise different clonal types, some of which may participate in disease. the purpose of the present investigation was to use restriction endonuclease analysis (rea) and ribotyping to characterize f. nucleatum clonal types isolated from healthy ...19989689613
experimental root canal infections in conventional and germ-free mice.a small animal model was evaluated to study the interrelationships between microorganisms after their implantation in root canals (inferior central incisors) using germ-free (gf) and conventional (cv) mice. the selected microorganisms were: porphyromonas endodontalis (atcc 35406), eubacterium lentum (atcc 25559), peptostreptococcus anaerobius (atcc 27337), fusobacterium nucleatum (atcc 10953), escherichia coli (atcc 25922), and enterococcus faecalis (atcc 4083). only p. anaerobius, e. coli, and ...19989693583
an aminopeptidase nutritionally important to fusobacterium nucleatum.the properties of an aminopeptidase (ap) from fusobacterium nucleatum were studied in view of the fact that this organism, along with other gram-negative anaerobes involved in periodontal diseases, survives in the subgingival environment by obtaining energy via the fermentation of a small number of peptide-derived amino acids. the ap was found to be cell-associated and was isolated from disrupted chemostat-grown cells. it was purified by (nh4)2so4 fractionation, two column chromatographic steps ...19989695913
bone resorption caused by three periodontal pathogens in vivo in mice is mediated in part by prostaglandin.gingival inflammation, bacterial infection, alveolar bone destruction, and subsequent tooth loss are characteristic features of periodontal disease, but the precise mechanisms of bone loss are poorly understood. most animal models of the disease require injury to gingival tissues or teeth, and the effects of microorganisms are thus complicated by host responses to tissue destruction. to determine whether three putative periodontal pathogens, porphyromonas gingivalis, campylobacter rectus, and fu ...19989712762
expression of the peptide antibiotic human beta-defensin 1 in cultured gingival epithelial cells and gingival tissue.human beta-defensin-1 (hbd-1) is a member of the family of small cationic antimicrobial peptides that have been identified in several mucosal epithelia. because human gingival epithelium is a site that is constantly challenged by oral microorganisms, we examined the expression of hbd-1 in human gingival epithelial and fibroblast cell cultures and tissue samples. cell cultures were challenged with cell wall extracts of porphyromonas gingivalis or fusobacterium nucleatum, escherichia coli lipopoly ...19989712771
in vitro activity of hmr 3647 against anaerobic bacteria.the aim of the present investigation was to determine the in vitro activity of hmr 3647 compared with other antimicrobial agents against anaerobic bacteria. the activity of hmr 3647 was determined against 342 clinical isolates of anaerobic bacteria by the agar dilution method and was compared with azithromycin, clarithromycin, roxithromycin, erythromycin, cefoxitin, imipenem, clindamycin and metronidazole. among the macrolides hmr 3647 and among the beta-lactams imipenem were the most active age ...19989720465
superficial suppurative thrombophlebitis in children, caused by anaerobic bacteria.the purpose of this study was to present the microbiology and clinical features of six children with superficial suppurative thrombophlebitis (sst) caused by anaerobic bacteria.19989722004
subgingival microbial profile of papillon-lefèvre patients assessed by dna-probes.the prevalence of 18 selected bacterial species was assessed by means of "checkerboard" dna-dna hybridisation in a group of 12 saudi-arabian adolescents with papillon-lefèvre syndrome. a total of 36 tooth sites were investigated. the patients exhibited severe periodontal disease with deep pockets. all 12 patients harboured the putative bacterial pathogens p. intermedia, f. nucleatum, p. micros and s. intermedius while t. denticola, b. forsythus, p. nigrescens, e. corrodens, s. noxia and c. rectu ...19989722266
oral microbiota in smokers and non-smokers in natural and experimentally-induced gingivitis.the present study primarily aimed at investigating the oral microbiota in smokers and non-smokers with established gingivitis and monitoring its composition during experimental gingivitis. secondly, it aimed at examining whether the composition of the microbiota is associated with different levels of gingival inflammation during this experimental gingivitis trial. for this purpose, 25 non-dental university students with gingivitis were recruited. 11 subjects were smokers and 14 were non-smokers. ...19989722273
preterm birth: associations with genital and possibly oral microflora.opportunistic pathogenic microbes are indigenous to the female lower genital tract and etiologic in many types of pelvic infections and, apparently, a portion of preterm birth (ptb) cases. bacterial vaginosis (bv) is a clinical syndrome based on an altered genital microflora in which gardnerella vaginalis; anaerobic species primarily among prevotella, porphyromonas, bacteroides, peptostreptococcus, and mobiluncus; mycoplasma hominis; and ureaplasma urealyticum become predominant in vaginal secre ...19989722706
anaerobic bacteremia and fungemia in patients undergoing endodontic therapy: an overview.oral focal infection, a concept neglected for several decades, is a subject of controversy. recent progress in classification and identification of oral microorganisms has renewed interest in focal infection. the aim of this study was to use phenotypic and genetic methods to trace microorganisms released into the bloodstream during and after endodontic treatment back to their presumed source--the root canal. microbiological samples were taken from the root canals of 26 patients with asymptomatic ...19989722712
early-onset periodontitis associated with down's syndrome--clinical interventional study.individuals with down's syndrome (ds) have an increased prevalence of periodontal disease compared with otherwise normal, age-matched control groups and other mentally handicapped patients of similar age distribution. the exaggerated immune-inflammatory response of the tissues cannot be explained by poor oral hygiene alone and might be the result of an impaired cell-mediated and humoral immunity and a deficient phagocytic system. as far as the progression and severity of destruction, the oral ma ...19989722720
analysis of ph-driven disruption of oral microbial communities in vitro.previously, a mixed culture chemostat system was used to demonstrate that the ph generated from carbohydrate metabolism, rather than carbohydrate availability per se, was responsible for the shifts observed in the oral microflora which are associated with high carbohydrate diets and the development of dental caries. the aim of this study was to determine more accurately the microbially generated ph at which such shifts occurred. nine oral bacteria were grown in three independent chemostats, and ...19989745120
role of fusobacterium nucleatum and coaggregation in anaerobe survival in planktonic and biofilm oral microbial communities during aeration.coaggregation is a well-characterized phenomenon by which specific pairs of oral bacteria interact physically. the aim of this study was to examine the patterns of coaggregation between obligately anaerobic and oxygen-tolerant species that coexist in a model oral microbial community. obligate anaerobes other than fusobacterium nucleatum coaggregated only poorly with oxygen-tolerant species. in contrast, f. nucleatum was able to coaggregate not only with both oxygen-tolerant and other obligately ...19989746571
activities of hmr 3004 (ru 64004) and hmr 3647 (ru 66647) compared to those of erythromycin, azithromycin, clarithromycin, roxithromycin, and eight other antimicrobial agents against unusual aerobic and anaerobic human and animal bite pathogens isolated from skin and soft tissue infections in humans.the activities of hmr 3004 and hmr 3647 and comparator agents, especially macrolides, were determined by the agar dilution method against 262 aerobic and 120 anaerobic strains isolated from skin and soft tissue infections associated with human and animal bite wounds. hmr 3004 and hmr 3647 were active against almost all aerobic and fastidious facultative isolates (mic at which 90% of the isolates are inhibited [mic90], < or = 0.5 and 1 microg/ml, respectively) and against all anaerobes [bacteroid ...19989593139
volatile fatty acid, metabolic by-product of periodontopathic bacteria, induces apoptosis in wehi 231 and raji b lymphoma cells and splenic b cells.the ability of butyric acid, an extracellular metabolite from periodontopathic bacteria, to induce apoptosis in murine wehi 231 cells, splenic b cells, and human raji cells was examined. the culture filtrate of porphyromonas gingivalis, prevotella loescheii, and fusobacterium nucleatum, which contains high a percentage of butyric acid, induced dna fragmentation in wehi 231 cells. volatile fatty acid, especially butyric acid, significantly suppressed b-cell viability in a concentration-dependent ...19989596720
granulocyte-macrophage colony-stimulating factor amplification of interleukin-1beta and tumor necrosis factor alpha production in thp-1 human monocytic cells stimulated with lipopolysaccharide of oral microorganisms.cytokines, including granulocyte-macrophage colony-stimulating factor (gm-csf), are used to assist in bone marrow recovery during cancer chemotherapy. interleukin-1beta (il-1beta) and tumor necrosis factor alpha (tnf-alpha) play important roles in inflammatory processes, including exacerbation of periodontal diseases, one of the most common complications in patients who undergo this therapy. a human monocyte cell line (thp-1) was utilized to investigate il-1beta and tnf-alpha production followin ...19989605989
studies on fusobacteria associated with periodontal diseases.the physiological and metabolic characteristics of representative isolates of the various subspecies of fusobacterium nucleatum were investigated by growing them in continuous culture in chemically-defined, media. behaving almost identically, these organisms were found to obtain energy from the fermentation of simple carbohydrates such as glucose or fructose or from the fermentation of certain amino acids, free or in the form of small peptides. the latter can be attacked by aminopeptidase activi ...19989612984
development of an anaerobic bacterial leakage model.the majority of bacteria associated with infections of endodontic origin are strict anaerobes. the purpose of this study was to develop an endodontic microleakage model using strict anaerobic bacteria in a two-chamber system. nine species of anaerobic bacteria were tested for viability and detection by either turbidity or color change of the broth. a survey of ph chromogenic substrates revealed that bromcresol purple (ph 5.2 = yellow, ph 6.8 = purple) could be used as a chromogenic indicator to ...19989641124
the effect of herbal extracts in an experimental mouthrinse on established plaque and gingivitis.the purpose of the present study was to establish in vitro the inhibiting effect of a herbal extract mixture on a selected number of micro-organisms and to test in vivo the effect of a mouthwash containing 6.3 mg/ml herbal extract mixture on plaque and gingivitis as compared to a minus active control mouthrinse. the herbal extract was a mixture of: juniperus communis (juniper), urtica dioca (nettle), achillaea millefolium (yarrow); 1:1:1. in the study, in-vitro, the effect of pure herbal extract ...19989650877
subgingival microflora associated with nifedipine-induced gingival overgrowth.the purpose of this study was to examine the composition of subgingival plaque of 140 periodontal lesions in 35 patients with cardiovascular disorders who were administered nifedipine and manifested nifedipine-induced gingival overgrowth (go). age was inversely associated with the go. plaque index and bleeding index showed a significant association with go, while nifedipine dosage and duration of nifedipine therapy were not found to be significant predictors of go. the gingival inflammation as e ...19989660335
severity of localized juvenile periodontitis as related to polymorphonuclear chemotaxis and specific microbial isolates.the purpose of this study was twofold: (a) to determine if an association exists between severity of localized juvenile periodontitis (ljp) and impairment of polymorphonuclear leukocyte (pmn) chemotaxis and/or colonization by specific microbial isolates; (b) to determine if the number of specific microbial isolates, i.e., actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, eikenella corrodens, and campylobacter rectus correlates to clin ...19989663117
cytotoxic effect of endodontic bacteria on periapical fibroblasts.this study was conducted to investigate the effects of sonicated bacterial extracts (sbes) from anaerobic gram-negative bacteria on periapical fibroblast obtained from the apical portion of human periodontal ligaments. porphyromonas endodontalis, porphyromonas gingivalis, prevotella intermedia, and fusobacterium nucleatum were chosen from among the endodontic bacteria isolated from root canals having a periapical lesion and compared in terms of their cytotoxicity. the purpose of this study was t ...19989759015
sinusitis in neurologically impaired children.the microbiologic features of infected sinus aspirates in nine children with neurologic impairment were studied. anaerobic bacteria, always mixed with aerobic and facultative bacteria, were isolated in 6 (67%) aspirates and aerobic bacteria only in 3 (33%). there were 24 bacterial isolates, 12 aerobic or facultative and 12 anaerobic. the predominant aerobic isolates were klebsiella pneumoniae, escherichia coli, and staphylococcus aureus (2 each) and proteus mirabilis, pseudomonas aeruginosa, hae ...19989781990
microbiological findings in prepubertal periodontitis. a case report.generalized pre-pubertal periodontitis (gpp) is a rare entity that usually affects children with severe systemic diseases. we report the case of a 7-year-old male patient diagnosed with gpp, with no apparent systemic condition, who lost all his primary teeth to periodontal disease. before extractions, while he was still in mixed dentition the subgingival plaque was collected and analyzed using dna probes to 40 different microorganisms. putative periodontopathogens such as prevotella intermedia, ...19989802719
functional changes in thp-1 human monocytic cells after stimulation with lipopolysaccharide of oral microorganisms and granulocyte macrophage colony stimulating factor.a human thp-1 monocyte cell line culture system has been utilized to observe the effect of granulocyte macrophage colony stimulating factor (gm-csf) supplementation with lipopolysaccharide (lps) of oral microorganisms to stimulate monocyte/macrophage functional activity. lps of oral microorganisms, fusobacterium nucleatum and porphyromonas gingivalis was produced by phenol-water extraction and characterized. the phagocytosis assay was performed using f1tc labeled saccharomyces yeast particles. p ...19989805230
bacterial eradication from root dentine by ultrasonic irrigation with sodium hypochlorite.the study aimed to evaluate intracanal irrigation procedures in eradicating bacteria from surface, shallow and deep layers of root dentine using extracted human teeth. artificial bacterial smear layer was successfully produced by rubbing a mixture of dental plaque and artificially decalcified dentine or carious dentine on root canal walls. the reservoir holes were 3.5 mm in depth, 1 mm in diameter prepared 1.5 mm apart and parallel to the root canals on the decrowned planes, in which five separa ...19989823113
polymicrobial coronal leakage of super eba root-end fillings following two methods of root-end preparation.the aim of this study was to compose in vitro coronal leakage of a super eba root-end filling material after two root-end cavity preparation techniques. a mixed anaerobic microbial marker was used. forty-five extracted human teeth with straight, single root canals were prepared chemo-mechanically to a size 40 master apical file. the teeth were divided into experimental groups (35 teeth) and control groups (10 teeth). forty teeth (35 experimental teeth and five negative control teeth) were obtura ...19989823138
[a comparative in-vitro study of the bactericidal efficacy of sodium hypochlorite and chlorhexidine gluconate plus cetrimide on root canal anaerobic bacterial flora].the permanence of necrotic tissues and/or bacteria may be responsible for persistent root canal infection. root canal irrigation plays an important role in the success of endodontic treatment given that, on the one hand, it encourages the progressive elimination of the smear layer and, on the other, it neutralises microbic flora within the canal. the aim of this study was to test root canal irrigants from a microbiological point of view: sodium hypochlorite 5% (niclor ogna, milan) and a new gene ...19989835745
additional clinical and microbiological effects of amoxicillin and metronidazole after initial periodontal therapy.the aims of this study were to evaluate the clinical and microbiological effects of initial periodontal therapy (it) and to determine the additional effects of systemic amoxicillin (flemoxin solutab) 375 mg tid plus metronidazole 250 mg tid therapy, in patients with adult actinobacillus actinomycetemcomitans (aa)-associated periodontitis in conjunction with either porphyromonas gingivalis (pg), bacteroides forsythus (bf) and/or prevotella intermedia (pi). in addition the adverse effects of the a ...19989846793
septic arthritis due to fusobacterium nucleatum. 19989851282
preterm labor and bacterial intraamniotic infection: arachidonic acid liberation by phospholipase a2 of fusobacterium nucleatum.the studies presented in this report were undertaken to evaluate whether fusobacterium nucleatum, a common anaerobic isolate in intrauterine infection, stimulates arachidonic acid metabolism, a rate-limiting step for prostaglandin synthesis, in the human uterine endometrium.19989855600
analysis of serum antibody responses to periodontopathogens in early-onset periodontitis patients from different geographical locations.serum antibody specificity to oral micro-organisms was used to delineate the pathogens associated with early-onset periodontal diseases in a turkish population. additionally, comparison of the findings to those derived from a clinically similar us patient population described differences in bacterial specific antibody between these 2 geographic regions. serum from 89 (ljp), 86 (rpp) and 94 (normal) subjects was analyzed (elisa) to determine igg antibody to 14 oral micro-organisms. all ljp patien ...19989869349
identification of highly fucosylated n-linked oligosaccharides from the human parotid gland.the glycosylation of a number of constituents of human saliva is known to modify its biological roles, such as its lubricating properties and binding of microbial flora. gillece-castro et al. [gillece-castro, b. l., prakobphol, a., burlingame, a. l., leffler, h. & fisher, s. j. (1991) j. biol. chem. 266, 17358-17368] have proposed that the major glycan on the salivary proline-rich glycoproteins is a trifucosylated biantennary sugar with one difucosylated and one unfucosylated antenna. furthermor ...19989874230
preparation and characterization of an actinomyces naeslundii aggregation factor that mediates coaggregation with porphyromonas gingivalis.intergeneric coaggregation is responsible for the complexity of the microbiota in human dental plaque and is believed to be important in the initial bacterial colonization of the human oral cavity. actinomyces naeslundii, an early colonizer of the tooth surface, may enhance subsequent colonization by porphyromonas gingivalis which is associated with adult periodontitis. the purpose of this study was to isolate and characterize the a. naeslundii aggregation factor (anaf) that mediates coaggregati ...19989879519
in vitro determination of direct antimicrobial effect of calcium hydroxide.the objective of this study was to determine in vitro the time required for calcium hydroxide in direct contact with microorganisms to express its antimicrobial effect. the microorganisms used were: micrococcus luteus (atcc-9341), staphylococcus aureus (atcc-6538), fusobacterium nucleatum (atcc-25586), pseudomonas aeruginosa (atcc-27853), escherichia coli, and streptococcus sp. the strains were cultivated in brain heart infusion (bhi), with the exception of f. nucleatum (bhi-pras). pure and mixe ...19989487859
microbial complexes in subgingival plaque.it has been recognized for some time that bacterial species exist in complexes in subgingival plaque. the purpose of the present investigation was to attempt to define such communities using data from large numbers of plaque samples and different clustering and ordination techniques. subgingival plaque samples were taken from the mesial aspect of each tooth in 185 subjects (mean age 51 +/- 16 years) with (n = 160) or without (n = 25) periodontitis. the presence and levels of 40 subgingival taxa ...19989495612
subgingival distribution of periodontopathic bacteria in adult periodontitis and their susceptibility to minocycline-hcl.the purpose of this study was to investigate the distribution of several periodontopathic bacteria in adult periodontitis, their in vitro susceptibility to minocycline-hcl, and whether the efficacy of the drug changes with a decrease in bacterial susceptibility. twenty-one patients (43 to 75 years old) with 62 periodontal lesions from pockets > or =4 mm participated in the study. after subgingival sampling, an ointment containing 2% minocycline-hcl was applied locally to the selected pockets onc ...19989527568
killing of fusobacterium nucleatum, porphyromonas gingivalis and prevotella intermedia by protegrins.protegrins are broad spectrum antibiotic peptides isolated from porcine leukocytes. in this study, we (i) examine the sensitivity of gram-negative, anaerobic periodontal pathogens to synthetic protegrins; (ii) determine the relative potencies of protegrin congeners against these bacteria; and (iii) compare the potency of protegrins with other antibiotic peptides, including magainin msi-78, tachyplesin i, cecropin p1, human defensins hnp-1-3, and clavanin a. synthetic l- and d-enantiomers of prot ...19989553868
perforation repair comparing mineral trioxide aggregate and amalgam using an anaerobic bacterial leakage model.the purpose of this study was to evaluate the ability of mineral trioxide aggregate (mta) and amalgam to seal furcal perforations in extracted human molars using an anaerobic bacterial leakage model. furcal perforations were made in 39 maxillary and mandibular human molars with a high-speed bur. these were randomly divided into two experimental groups of 18, with the remaining three teeth used as positive controls. experimental group 1 was repaired with mta and group 2 with amalgam. three additi ...19989558584
beta-sheet antibiotic peptides as potential dental therapeutics.small, cysteine-rich, beta-sheet peptide antibiotics are found throughout the animalia. though broad spectrum in potential, they may exert selective antimicrobial effects under certain conditions. we have explored the antimicrobial properties of two families of beta-sheet peptide antibiotics, defensins and protegrins, against periodontopathic bacteria. the rabbit defensin np-1 was active against facultative gram-negative bacteria associated with early onset periodontitis, including actinobacillu ...19989573496
effect of red blood cells on the growth of porphyromonas endodontalis and microbial community development.establishment of a microbial community in the root canal system depends on numerous factors, of which nutrient availability may be one of the most important. we hypothesized that the presence of red blood cells or hemoglobin in this environment could cause shifts in microbial composition of communities, resulting in organisms such as porphyromonas endodontalis becoming more dominant. an in vitro model system using mixed, batch cultures was performed with the bacteria p. endodontalis, fusobacteri ...19989573801
antianaerobic activity of a cecropin---melittin peptide.objective: several small, 15-residue peptides that contain portions of the amino acid sequences of both cecropin a and melittin have previously been shown to have broad-spectrum antibacterial activities against aerobic microorganisms, with no undesirable hemolytic properties. it would also be useful to know what effect these hybrid peptides have on anaerobic bacteria. methods: the minimum inhibitory concentrations of one hybrid, ca(1--7)m(2--9)nh2, were compared with those of seven other antimic ...199811864323
c-telopeptide pyridinoline cross-links (ictp) and periodontal pathogens associated with endosseous oral implants.detection of periodontal or peri-implant sites exhibiting progressing disease or those at risk of deterioration has proven difficult. pyridinoline cross-linked carboxyterminal telopeptide of type i collagen (ictp), a marker specific for bone degradation found in gingival crevicular fluid (gcf), has been associated with both bone and attachment loss in periodontitis and may be useful for predicting disease activity. the aim of this cross-sectional study was to examine the relationship between ict ...199811429938
the behaviour of fusobacterium nucleatum chemostat-grown in glucose- and amino acid-based chemically defined media.fusobacterium nucleatum is a gram-negative anaerobe, found in a number of different areas of human and animal bodies as part of the resident microbiota. however, it also appears to be involved in polymicrobial infections in such sites. it occurs in the oral cavity where it is a prominent member of various bacterial consortia associated with periodontal diseases. like most fusobacteria, it derives energy via the fermentation of amino acids which it can obtain through the dissimilation of small pe ...199816887630
a report on genetic registers. based on the report of the clinical genetics society working party. 1998106123
[colo-rectal tumors. methods and indications for their early diagnosis]. 1998108582
total nose reconstruction: a single stage method. 1998105856
isolation of nucleoli from tetrahymena pyriformis. 1998108165
unsuspected aortic dissection: the chronic "healed" dissection.of all aortic dissections, 10% are chronic. typically they arise distal to the left subclavian artery and have reentry points into the true lumen. pain may be minimal or absent and patients often present with cardiac failure. chronic dissections are more likely to appear radiographically as atherosclerotic aneurysms on a chest film than are acute dissections. four cases of chronic dissections found incidentally during angiography are presented.1998105588
diagnostic significance of the passive hemagglutination tests in penicillin and aspirin allergy. 1998106406
[coumarin derivatives for long-term therapy of coronary heart disease]. 1998108500
chloramphenicol and tetracycline usage in outpatient practice. 1998121377
[complications in laparoscopic interventions and how to prevent them]. 1998124827
[c-peptide, igg-insulin antibodies, island cell antibodies and immune response genes in diabetes mellitus]. 1998105488
a further observation on gallbladder debris. 1998108315
[role of epidemiological models in surveillance of communicable diseases (author's transl)].the interpretation of basic information obtained through surveillance of communicable diseases requires knowledge of the expected trend of the epidemiological phenomena under observation. the more precise the epidemiological forecasts are, the more efficient the methods of surveillance will be. with a few examples the author describes briefly the role of epidemiological models to produce reliable previsions, the principles ruling their construction, their use on computer to simulate known epidem ...1998106444
effects of alcohol abuse on progression of liver disease in methadone-maintained patients. 1998121359
hairy cell leukemia: a clinical, immunological and ultrastructural study. new data for the origin of the hairy cell.seven patients presenting with the characteristic clinical and laboratory findings of hcl were investigated mainly in order to find the origin of the hc with histochemical, electron microscopic and immunologic techniques. the results we obtained are somewhat contradictory, as in the recent literature, if the hc's are to the considered as being exclusively of lymphocytic or monocytic origin. the data however indicate that hcl is not of one cell type but two. one of these cells has characteristics ...199898909
studies on the allosteric properties of glycogen synthase i. 1998108102
anion exchange chromatographic separation of inositol phosphates and their quantification by gas chromatography.the direct measurement of mass of inositol trisphosphate from biologic samples is described. separation of inositol monophosphate, bisphosphate, trisphosphate, and inositol tetrakisphosphate was achieved using anion exchange chromatography with a sodium sulfate gradient. in addition, separation of the isomers of each inositol phosphate was performed using hplc procedures. the individual inositol phosphate fractions were subsequently dephosphorylated and desalted. the myo-inositol from each fract ...19982653097
[stability and reliability of a brief-intelligence test (mwt-b) to long-stay psychiatric patients (author's transl)]. 1998108605
some statistics on 2,810 cases of epilepsy. 1998104836
validating audit criteria: an analytic approach illustrated by peptic ulcer disease. 1998121383
evaluation of the bactericidal activity on oral organisms of the nd-yap laser in vitro.the bactericidal activity of a pulsed fibre-optic nd-yap laser on various strains of oral bacteria: prevotella nigrescens, streptococcus sanguis, actinomyces viscosus (i), fusobacterium nucleatum, actinobacillus actinomycetemcomitans and peptostreptococcus micros (i) was evaluated. a 200-μm optical fibre was placed in eppendorf tubes containing the different bacterial suspensions. at an energy of 170 mj/30 hz, 351 pulses of 150 μs were sufficient to kill the most resistant bacteria (i). the expo ...199824710990
beta-lactamase inhibitors--a novel approach. 199989565
vitiligo patterns simulating autoimmune and rheumatic diseases.several variants of generalised vitiligo can be recognised by their cutaneous distribution. in some, certain anatomical regions--e.g., hands--are predominantly affected while the cutaneous depigmentation in other variants shows a similar anatomical distribution to that of the internal structures affected in some of the autoimmune and rheumatic diseases. five patients have been seen, and three described by others, with vitiligo affecting skin of eyelids and lower front of neck, reminiscent of the ...199989452
continuous subcutaneous insulin infusion or subcutaneous insulin injections. 199989550
[intraosseous administration of thymalin in the combined treatment of children with acute odontogenic osteomyelitis].experience gained in the treatment of 63 children has demonstrated the validity of bone perforation and intraosteal drug lavage in a complex of therapeutic measures for acute mandibular odontogenic osteomyelitis running an unfavorable course. incorporation of immunostimulant thymalin in the solutions used for intraosteal lavage of the inflammation focus in this condition is conducive to normalization of the cellular and humoral immunity and liquidation of the pathologic process without destructi ...19992326818
frankly speaking: economics: nursing's friend or foe? 199996934
discipline without punishment--part i. 19992586930
preoperative haemoglobin as predictor of outcome of diabetic amputations.simple factors which may serve as predictors of the success or failure of amputations in the feet were examined in 59 consecutive diabetics. age, sex, method of diabetic control, smoling, presence of neuropathy or peripheral pulses, preoperative blood-urea, and temperature did not correlate with success of amuptations. the average preoperative white-cell count and blood-sugar were higher in the failure group (p less than 0.01 and p less than 0.05 respectively), but there was considerable overlap ...199989280
fatal leishmaniasis in renal-transport patient. 199988649
deaths of infants after triple vaccine. 199989408
peripheral and central 5-hydroxytryptamine in trisomy 21. 199993675
new screening test for colorectal cancer developed in canada. 199989207
effect on bone of withdrawal of oestrogen therapy. 199989300
hexacarbon neuropathy. 199991030
diagnostic value of biochemical analysis of pleural effusions. carcinoembryonic antigen and beta 2 microglobulin.pleural effusions from 105 patients with malignant and nonmalignant diseases were examined for tumor cells, content of cea, beta2 microglobulin, ceruloplasmin, alpha2 macroglobulin, orosomucoid, lysozyme, and hexosaminidase. only cea and beta2 microglobulin determinations were of diagnostic value. cea concentrations greater than 11 ng/ml were found only in malignant effusions. beta 2 microglobulin values were increased in pleural effusions due to lymphoma or immune diseases. measurement of cea a ...199985412
plasma 1,25(oh)2 vitamin d concentrations in nutritional osteomalacia. 199989299
ballot in scotland on proposed consultant contract. 199991768
an evaluation of surgical and conservative treatment of fractures of the ankle in 200 patients.the longterm results of surgical and conservative treatment in two almost identical groups of patients with fractures of the ankle have been analyzed. the results were better after conservative treatment; delayed operation and repeated manipulations were found to be the main reason for the poorer postoperative results, especially in elderly osteoporotic patients. the damage consequent to repeated manipulations, the difficulties resulting from delaying operation, and the advantages of early open ...199996272
hermann zondek. 199989329
[determination of serum immunoglobulin level in patients with immunologic deficiency by thermistography]. 199989257
malabsorption syndrome in ibadan. 199997069
cell communication, cell union, and initiation of meiosis in ciliate conjugation. 199997053
[pathogenesis of pancreatitis (author's transl)]. 199996436
multiple astrocytoma of spinal cord in von-recklinghausen's disease. a case report. 199996357
candida dubliniensis and candida albicans display surface variations consistent with observed intergeneric coaggregation.adherence of yeasts to other microorganisms and epithelial cell surfaces is important in their colonization. comparative studies based on the coaggregation of candida dubliniensis versus candida albicans with fusobacterium nucleatum and other oral bacteria suggested differences in the surfaces of these yeasts. transmission electron microscopy was used to test the hypothesis that there are morphologic variations in the cell surface of these two species. c. dubliniensis type strain cd36 and c. alb ...199918473545
beta-lactamases producing anaerobic bacteria in dentoalveolar abscesses.pus samples of 30 patients with closed dentoalveolar abscesses who had not received antimicrobial therapy for at last two months were screened for the presence of beta-lactamase-producing anaerobic bacteria. from these 30 pus samples, a total of 112 bacterial strains were isolated; 83 of them were strict anaerobes and 29 were aerobes. beta-lactamases activity of the selected anaerobic bacteria was tested after identification of the isolates and was detected in 5 of the total 83 (6%) strict anaer ...199910693296
[anaerobic infection and its rapid detection in surgical patients].to investigate anaerobic infection and its rapid detection in surgical patients.199911829950
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