PMID(sorted descending)
native plasmids of fusobacterium nucleatum: characterization and use in development of genetic systems.three native plasmids of fusobacterium nucleatum were characterized, including dna sequence analysis of one plasmid, pfn1. a shuttle plasmid, phs17, capable of transforming escherichia coli and f. nucleatum atcc 10953 was constructed with pfn1. phs17 was stably maintained in the f. nucleatum transformants, and differences in the transformation efficiencies suggested the presence of a restriction-modification system in f. nucleatum.200010648549
in vitro volatile sulfur compound production of oral bacteria in different culture media.the purpose of this study was to detect the relative contribution of porphyromonas gingivalis, fusobacterium nucleatum, and prevotella intermedia in the production of oral malodor.199910635291
comparing periodontal disease in identical twins: a case report.previous investigators have shown that numerous environmental and genetic variables may contribute to the pathogenesis of periodontal disease. this case report presents clinical and laboratory findings of a set of caucasian female identical twins. one patient presented clinically with mild gingivitis and no clinical or radiographic signs of periodontitis. the other exhibited gingivitis with localized, moderate-to-severe periodontitis. neither patient reported a history of systemic conditions tha ...199910634119
peptostreptococcus micros coaggregates with fusobacterium nucleatum and non-encapsulated porphyromonas gingivalis.coaggregation is one of the potential colonization strategies of oral microorganisms, often involving fimbrial structures in the interactions. in this study, the coaggregation characteristics of the rough and smooth genotypes of the periodontal pathogen peptostreptococcus micros were compared to investigate the role of the fibril-like structures of the rough genotype in coaggregation. of the 11 oral species tested, only fusobacterium nucleatum strains and non-encapsulated porphyromonas gingivali ...200010612731
pre- and post-implantation microbiota of the tongue, teeth, and newly placed implants.this investigation sought intra-oral sources of species colonizing dental implants.199910599911
longitudinal study of dental implants in a periodontally compromised population.the purpose of this longitudinal study was to determine the clinical status and the composition of the subgingival microbiota of dental implants and natural teeth in patients with a history of periodontitis.199910588495
fusobacterium nucleatum empyema necessitans. 199910585835
acquisition of plasmin activity by fusobacterium nucleatum subsp. nucleatum and potential contribution to tissue destruction during periodontitis.fusobacterium nucleatum subsp. nucleatum has been associated with a variety of oral and nonoral infections such as periodontitis, pericarditis, bone infections, and brain abscesses. several studies have shown the role of plasmin, a plasma serine protease, in increasing the invasive capacity of microorganisms. in this study, we investigated the binding of human plasminogen to f. nucleatum subsp. nucleatum, and its subsequent activation into plasmin. plasminogen-binding activity of bacterial cells ...199910569761
nosocomial sinusitis with isolation of anaerobic bacteria in icu determine the frequency and the eventual clinical characteristics of nosocomial sinusitis with anaerobic bacteria isolation in patients in the intensive care unit (icu).199910551960
enhanced interleukin-8 production in thp-1 human monocytic cells by lipopolysaccharide from oral microorganisms and granulocyte-macrophage colony-stimulating factor.granulocyte-macrophage colony-stimulating factor (gm-csf) has been used to assist in bone marrow recovery during cancer chemotherapy. interleukin-8 (il-8) plays an important role in macrophage mediated inflammatory processes including exacerbation of periodontal diseases, one of the most common complications in gm-csf receiving cancer patients. the effect of gm-csf supplementation on il-8 production was investigated in a human monocyte cell line thp-1, stimulated with lipopolysaccharide extracte ...199910551153
infective endocarditis due to fusobacterium nucleatum in an intravenous drug abuser.infective endocarditis due to anaerobic non-spore-forming gram-negative bacilli in intravenous drug abusers is exceedingly rare, with only two cases being previously reported in the literature. a case of endocarditis due to fusobacterium nucleatum in an intravenous drug abuser is reported, accompanied by a review of the literature.199910534188
arbitrarily primed-polymerase chain reaction for identification and epidemiologic subtyping of oral isolates of fusobacterium nucleatum.fusobacterium nucleatum is the most frequently isolated bacterium in periodontal disease and plays an important role in serious infections in other parts of the body. arbitrarily primed-polymerase chain reaction (ap-pcr) was used to construct primers for specific identification and subtyping of f. nucleatum. subtypes may differ in virulence and, hence, are important as periodontal pathogens. subtypes also may differ in antibiotic susceptibility; therefore, knowing the subtypes may influence choi ...199910534075
dexamethasone differentially regulates cytokine transcription and translation in macrophages responding to bacteria or okadaic acid.many microorganisms and microbial products induce expression of pro-inflammatory cytokines such as interleukin-1 (il-1alpha/beta) and tumour necrosis factor-alpha (tnf-alpha) in macrophages, primarily by transcriptional activation. we show here, by using mouse macrophages in primary culture, that pre-treatment with dexamethasone inhibits bacteria-induced il-1beta expression as mrna and cellular pro-il-1beta in parallel, consistent with an effect primarily on transcriptional activation. in contra ...199910530874
an evaluation of the action of different root canal irrigants on facultative aerobic-anaerobic, obligate anaerobic, and microaerophilic bacteria.the aim of this study was to test the effect of different concentrations of sodium hypochlorite, chlorhexidine, and cetrimide on the following bacterial strains: facultative aerobes-anaerobes: candida albicans atcc 10231; enterococcus faecalis atcc 29212; escherichia coli atcc 25,922; pseudomonas aeruginosa atcc 27,853; streptococcus mitis atcc 9811; streptococcus mutans atcc 35668; streptococcus salivarius atcc 13419; and streptococcus sanguis atcc 10556. microaerophiles: actinobacillus actinom ...199910530260
utilization of d-amino acids by fusobacterium nucleatum and fusobacterium varium.the utilization of d- and l-amino acids with acidic, basic or polar side chains was demonstrated by hplc. two species of the anaerobe fusobacterium utilized d-lysine and the l isomers of glutamate, glutamine, histidine, lysine and serine. only f. varium used l-arginine, d-glutamate and d-serine as substrates, whereas f. nucleatum specifically utilized d-histidine and d-glutamine. d-glutamate accumulated in f. nucleatum cultures supplemented with d-glutamine, and ornithine was detected when eithe ...199910524276
establishment of oral anaerobes during the first year of life.anaerobic species constitute a significant part of the bacterial community of the mouth. although the time and species involved in the primary colonization of infants are of great importance by forming the basis for further colonization, the development of the oral anaerobic microflora with age is still inadequately understood. in the present study, time and succession of colonization of oral anaerobes were longitudinally examined in 44 healthy caucasian infants at 2, 6, and 12 months of age. un ...199910520968
2-hydroxyglutaryl-coa dehydratase from clostridium symbiosum.component d (hgdab) of 2-hydroxyglutaryl-coa dehydratase from clostridium symbiosum was purified to homogeneity. it is able to use component a from acidaminococcus fermentans (hgdc) to initiate catalysis together with atp, mg2+ and a strong reducing agent such as ti(iii)citrate. component d from c. symbiosum has a 6 x higher specific activity compared with that from a. fermentans and contains a second [4fe-4s] cluster but the same amount of riboflavin 5'-phosphate (1.0 per heterodimeric enzyme, ...199910491198
microbiological features of papillon-lefèvre syndrome periodontitis.papillon-lefevre syndrome patients exhibit hyperkeratosis palmo-plantaris and severe periodontitis. the syndrome is an autosomal recessive trait, but the mechanism of periodontal destruction is not known. this report presents the clinical and microbiological features of an 11-year old girl with papillon-lefèvre syndrome. clinical examination included conventional periodontal measurements and radiographic analysis. in samples from 3 deep periodontal lesions, the occurrence of major suspected peri ...199910487314
primary psoas abscess due to fusobacterium nucleatum.a case of primary pyogenic psoas abscess due to fusobacterium nucleatum is described. clinicians must maintain a high index of clinical suspicion for the diagnosis of psoas abscess. although staphylococcus aureus accounts for most cases of primary psoas abscess, this report emphasizes the importance of bacteriological confirmation of the microorganism involved.199910482067
microbiological response of localized sites with recurrent periodontitis in maintenance patients treated with tetracycline fibers.whether adjunctive tetracycline fibers can provide an additive effect to scaling and root planing in treating non-responsive sites in maintenance subjects is still controversial. recolonization of the bacteria from untreated sites or from the extracrevicular region may explain the insignificant response to local therapy. the purpose of the present study was to evaluate the microbiological response of sites treated with tetracycline fibers combined with scaling and root planing.199910476893
in vitro activity of gemifloxacin (sb 265805) against anaerobes.gemifloxacin mesylate (sb 265805), a new fluoronaphthyridone, was tested against 359 recent clinical anaerobic isolates by the national committee for clinical laboratory standards reference agar dilution method with supplemented brucella blood agar and an inoculum of 10(5) cfu/spot. comparative antimicrobials tested included trovafloxacin, levofloxacin, grepafloxacin, sparfloxacin, sitafloxacin (du-6859a), penicillin g, amoxicillin clavulanate, imipenem, cefoxitin, clindamycin, and metronidazole ...199910471570
the effects of hiv viral load on the phagocytic activity of monocytes activated with lipopolysaccharide from oral microorganisms.a study was undertaken to determine whether viral load status in hiv+ patients has any potential effect on monocyte phagocytic function both before and after challenge of the monocytes with lipopolysaccharide (lps) isolated from oral microorganisms. lps of two putative periodontal pathogens porphyromonas gingivalis (p. gingivalis) and fusobacterium nucleatum (f. nucleatum) was prepared. whole blood samples in edta were collected from 30 hiv+ patients presenting for dental care at the university ...199910466072
fusobacterium nucleatum septicemia and portal vein thrombosis. 199910451179
infection-stimulated infraosseus inflammation and bone destruction is increased in p-/e-selectin knockout mice.infections of the dental pulp commonly result in infraosseus inflammation and bone destruction. however, the role of phagocytic leucocytes in the pathogenesis of pulpal infections has been uncertain. in this work we used p/e-/- selectin-deficient mice, which lack rolling adhesion of leucocytes to endothelium and mimic the human syndrome, leucocyte adhesion deficiency ii (lad-ii), to test the hypothesis that phagocytic leucocytes protect against pulpal infection and subsequent periapical infraoss ...199910447722
antigen activation of thp-1 human monocytic cells after stimulation with lipopolysaccharide from oral microorganisms and granulocyte-macrophage colony-stimulating factor.a human thp-1 monocyte cell line culture system has been utilized to evaluate the morphological changes in thp-1 cells and to measure expression of activation antigens (cd-11b, cd-11c, cd-14, cd-35, cd-68, cd-71 and hla-dr) as evidence of maturation of thp-1 cells in response to stimulation by lipopolysaccharide (lps) from the oral microorganisms, fusobacterium nucleatum and porphyromonas gingivalis, and granulocyte-macrophage colony-stimulating factor. thp-1 cells were stimulated with lps (1 mi ...199910444744
oral microflora in subjects with reduced salivary is generally assumed that a decreased salivary secretion rate will promote plaque accumulation and increase the risk for caries, gingival inflammation, and mucosal infections. in this study, the effect of hyposalivation on the oral microflora was examined. the following micro-organisms were analyzed in rinsing samples from 14 subjects with hyposalivation: the total number of anaerobically growing micro-organisms, alpha-hemolytic streptococci, mutans streptococci, lactobacilli, fusobacterium n ...199910439028
porphyromonas gingivalis prevalence related to other micro-organisms in adult refractory periodontitis.forty-six adult periodontal patients, selected on the basis of clinical examination, and 46 adult healthy subjects were examined. the subgingival plaque samples from one inflammatory and one non-inflammatory site of each periodontal patient were studied to determine porphyromonas gingivalis prevalence related to other periodontal micro-organisms and to periodontal tissue destruction. the results showed porphyromonas gingivalis as the main pathogenic micro-organism isolated in the inflammatory si ...199910423739
comparative antimicrobial activity of an essential oil and an amine fluoride/stannous fluoride mouthrinse in vitro.although laboratory studies are not necessarily predictive of clinical activity; they can help to elucidate mechanisms underlying clinical activity when the latter has been established. in a recent clinical study, an essential oil mouthrinse (listerine antiseptic) was shown to be significantly more effective than an amine fluoride/stannous fluoride mouthrinse (meridol) in inhibiting supragingival plaque formation. this paper reports the results of laboratory studies comparing the antimicrobial e ...199910412853
clinical and microbiological effects of initial periodontal therapy in conjunction with amoxicillin and clavulanic acid in patients with adult periodontitis. a randomised double-blind, placebo-controlled study.the aim of the present study was to investigate the clinical and microbiological effects of initial periodontal therapy in conjunction with systemic amoxicillin plus clavulanic acid in adult periodontitis patients using a double-blind, parallel-group, and placebo-controlled protocol. 21 patients with a clinical diagnosis of generalised adult periodontitis were recruited. clinical measurements and microbiological assessments were carried out at baseline, 3, and 12 months post-treatment. approxima ...199910412851
a 15-month evaluation of the effects of repeated subgingival minocycline in chronic adult periodontitis.a double-blind, randomized, parallel, comparative study was designed to evaluate the long-term safety and efficacy of subgingivally administered minocycline ointment versus a vehicle control.199910397521
beta-lactamase production by oral anaerobic gram-negative species in infants in relation to previous antimicrobial therapy.the frequency of beta-lactamase production in gram-negative bacteria has increased considerably during recent years. in this study, beta-lactamase production by oral anaerobic gram-negative rods isolated from saliva was longitudinally examined for 44 caucasian infants at the ages of 2, 6, and 12 months in relation to their documented exposure to antibiotics. isolates showing decreased susceptibility to penicillin g (1 microg/ml) were examined for beta-lactamase production by using a chromogenic ...199910390208
flow cytometry to monitor phagocytosis and oxidative burst of anaerobic periodontopathogenic bacteria by human polymorphonuclear leukocytes.the reduced susceptibility to phagocytosis found among some periodontopathogenic anaerobes may account for the differences between invasive and non-invasive strains. we applied flow cytometry as a powerful tool to analyze and quantify phagocytosis using standardized cultures of oral anaerobes (porphyromonas gingivalis, prevotella intermedia, p. nigrescens, capnocytophaga gingivalis, c. ochracea, c. sputigena, fusobacterium nucleatum and peptostreptococcus micros) and heparinized whole blood. bac ...199910384401
intra-familial distribution of fusobacterium nucleatum strains in healthy families with optimal plaque control.fusobacterium nucleatum, a gram-negative anaerobic rod associated with periodontal disease, is also found in healthy individuals and is considered part of the indigenous oral microflora. although intra-familial transmission of periodontal pathogens has been documented, there are no data relating transmission of f. nucleatum. this study investigated the distribution of f. nucleatum strains in 4 strictly healthy families. 32 f. nucleatum strains were isolated from 19 individuals (8 parents and 11 ...199910382581
antibiotic susceptibility of putative periodontal pathogens in advanced periodontitis the present study, the antibiotic susceptibility of most prevalent micro-organisms in advanced periodontitis patients was evaluated. in 56 patients, pooled subgingival plaque samples were taken from the deepest site of each quadrant and were cultivated anaerobically. from each patient, the 4 most frequently encountered types of bacterial colonies were subcultured and identified (rapid id 32 a). from all bacterial species identified in the 224 subcultures, the 4 most prevalent were used for su ...199910382573
the effects of histatin-derived basic antimicrobial peptides on oral biofilms.susceptibility of bacteria to antimicrobial agents is strongly reduced by the formation of complex biofilms. we investigated whether synthetic histatin analogs with broad-spectrum antibacterial activity in vitro were also active against these complex mixtures of bacteria, as present in saliva and plaque. in a simplified model system for dental plaque, hydroxyapatite discs were placed in a continuous culture system comprised of streptococcus mutans, s. sanguis, s. salivarius, actinomyces naeslund ...199910371248
linezolid activity compared to those of selected macrolides and other agents against aerobic and anaerobic pathogens isolated from soft tissue bite infections in humans.linezolid was tested against 420 aerobes and anaerobes, including 148 pasteurella isolates, by an agar dilution method. linezolid was active against all pasteurella multocida subsp. multocida and p. multocida subsp. septica isolates and most pasteurella canis, pasteurella dagmatis, and pasteurella stomatis isolates. the mic was </=2 microg/ml for staphylococci, streptococci, ef-4b, weeksella zoohelcum, fusobacterium nucleatum, other fusobacteria, porphyromonas spp., prevotella spp., peptostrepto ...199910348773
effects of hydrogen peroxide on growth and selected properties of porphyromonas this study we first evaluated the effects of hydrogen peroxide (h2o2) on growth and selected properties of porphyromonas gingivalis, and compared them with those obtained by a reducing agent (cysteine). the growth of p. gingivalis was only moderately affected when h2o2 was added at concentrations up to 30 mm in a complex culture medium. however, when a defined basal medium was used, h2o2 at a concentration of 3 mm completely inhibited growth of p. gingivalis. incorporation of cysteine at conc ...199910339828
abscess forming ability of streptococcus milleri group: synergistic effect with fusobacterium nucleatum.the abscess forming abilities of "streptococcus milleri" strains (streptococcus constellatus, streptococcus anginiosus, and streptococcus intermedius) isolated from dentoalveolar abscesses and the synergistic effect of fusobacterium nucleatum co-inoculated with the isolates were examined on a mouse subcutaneous abscess model. five days after inoculation, all s. milleri strains formed abscesses, which showed less pathological spread to surrounding connective tissues than those formed by staphyloc ...199910338189
fungi in therapy-resistant apical periodontitis.the occurrence of yeasts in 967 microbiological endodontic samples taken from root canals in persistent endodontic infections was studied. the sampling was done by general practitioners in various parts of finland from root canal infections which did not respond favourably to standard conservative therapy. the samples were cultivated aerobically on a non-selective enriched horse blood agar medium, on tsbv agar medium in 5% co2 and anaerobically on horse blood agar medium. micro-organisms were fo ...199710332243
microbiological and clinical effects of a 1% chlorhexidine-gel in untreated periodontal pockets from adult periodontitis patients.the aim of this study was to report the microbiological and clinical effects of repeated subgingival administration of a 1% chlorhexidine-gel in periodontal pockets from 10 patients with adult periodontitis. results showed that the experimental treatment significantly improved clinical parameters (plaque index, gingival bleeding index, and pocket probing depth). direct subgingival administration of chlorhexidine-gel also produced a remarkable modification in the proportions of putative periodont ...199910322610
periodontal bacteria in adult twins.both environmental and genetic factors are known to influence clinical measures of periodontal disease. the purpose of this study was to determine whether genetic factors similarly influence the presence of specific periodontal bacteria in subgingival plaque.199910225542
beta-lactamase production and antimicrobial susceptibility of oral heterogeneous fusobacterium nucleatum populations in young children.oral fusobacterium nucleatum populations from 20 young, healthy children were examined for beta-lactamase production. ten children (50%) harbored, altogether, 25 beta-lactamase-positive f. nucleatum isolates that were identified as f. nucleatum subsp. polymorphum, f. nucleatum subsp. nucleatum, and f. nucleatum subsp. vincentii (j. l. dzink, m. t. sheenan, and s. s. socransky, int. j. syst. bacteriol. 40:74-78, 1990). in vitro susceptibility of these beta-lactamase-producing and 26 non-beta-lact ...199910223950
induction of anti-thymocyte/t lymphocyte antibodies in mice injected with lipopolysaccharides from periodontopathic bacteria.we examined the levels of anti-thymocyte/t lymphocyte autoantibody (ata) in the serum of mice injected intraperitoneally with lipopolysaccharides (lps) from periodontopathic bacteria; porphyromonas gingivalis, actinobacillus actinomycetemcomitans, fusobacterium nucleatum, capnocytophaga ochracea, and non-oral escherichia coli. all of the lps induced igm-ata. among these, lps from c. ochracea induced the highest level of igm-ata, whereas that of p. gingivalis induced the lowest. the peritoneal t ...199910207839
coaggregation of candida dubliniensis with fusobacterium nucleatum.the binding of microorganisms to each other and oral surfaces contributes to the progression of microbial infections in the oral cavity. candida dubliniensis, a newly characterized species, has been identified in human immunodeficiency virus-seropositive patients and other immunocompromised individuals. c. dubliniensis phenotypically resembles candida albicans in many respects yet can be identified and differentiated as a unique candida species by phenotypic and genetic profiles. the purpose of ...199910203506
haemagglutination and haemolysis by oral fusobacterium nucleatum.haemagglutination and haemolytic activity of 80 fusobacterium nucleatum isolates from human and animal origin, on different human blood types was evaluated. all the isolates were able to agglutinate erythrocytes and the most were either alpha-haemolytic or beta-haemolytic. no specificity between haemolysin or haemagglutinin and blood type was observed. haemagglutination activity was inhibited when d-galactose, d-lactose or d-raffinose were used. haemagglutination and haemolysis may be important ...199910190119
identification of positively charged residues of foma porin of fusobacterium nucleatum which are important for pore function.foma porin is the major outer-membrane protein of fusobacterium nucleatum. it exhibits the functional properties of a general diffusion porin, but has no sequence similarity to other porins. according to the proposed topology model, each monomer of this trimeric protein is a beta-barrel consisting of 16 transmembrane segments with eight surface-exposed loops. several conserved charged residues are proposed to extend from the beta-barrel wall into the aqueous channel lumen, and may contribute to ...199910103012
microbiota of successful osseointegrated dental implants.the long-term survival of dental implants depends, in part, on control of bacterial infection in the peri-implant region. periodontal pathogens colonized implants symptomatic through infection, whereas the microbiota of successful implants was similar to that of periodontal health. this study examined the impact on the peri-implant microbiota of crown restorations; implant type; length of time of loading; history of implant or periodontal infections; and whether implants replaced single or multi ...199910102550
interleukin-6 production by human monocytes treated with granulocyte-macrophage colony-stimulating factor in the presence of lipopolysaccharide of oral microorganisms.this study focused on the effect of granulocyte-macrophage colony-stimulating factor (gm-csf) and lipopolysaccharide of the putative periodontal pathogens porphyromonas gingivalis or fusobacterium nucleatum on il-6 production by thp-1 cells (a human monocytic cell line). resting thp-1 cells were alternatively treated with gm-csf (50 iu/ml) and lipopolysaccharide of p. gingivalis or f. nucleatum, in varying concentrations for varying time periods. il-6 production in supernatant fluids of treated ...199810093532
immune response to fusobacterium nucleatum and prevotella intermedia in patients with chronic maxillary sinusitis.our objective was to study the immune response to 2 anaerobic bacteria in patients with chronic maxillary sinusitis. immunoglobulin g class antibody titers to fusobacterium nucleatum and prevotella intermedia were measured by enzyme-linked immunosorbent assay. serum levels in the patients were determined on the day sinus aspiration for culture was done, and 75 to 90 days later, after completion of antimicrobial therapy, prevotella intermedia was isolated from 16 aspirates, and f. nucleatum from ...199910086624
dominant cross-reactive antibodies generated during the response to a variety of oral bacterial species detect phosphorylcholine.the intraperitoneal immunization of balb/c mice with subgingival plaque from advanced periodontal pockets or with certain strains of actinobacillus actinomycetemcomitans, fusobacterium nucleatum, actinomyces israelii, streptococcus mitis, or streptococcus oralis yielded frequently indistinguishable igm monoclonal antibodies which were reactive with antigens from a variety of oral bacteria. this study aimed to characterize the specificity of such monoclonal antibodies and the diversity of oral ba ...199910065949
relationship of the subgingival microbiota to a chairside test for aspartate aminotransferase in gingival crevicular fluid.the aim of the present study was to evaluate the association between the occurrence of certain specific periodontal pathogens and aspartate aminotransferase (ast) levels in gingival crevicular fluid (gcf).199910052771
inflammatory cytokine production and specific antibody responses against possible causative bacteria in patients with multilesional periapical periodontitis.we examined the induction of inflammatory cytokines (including interleukin-1, interleukin-6, interleukin-8, and tumor necrosis factor-alpha) by several species of possible causative bacteria in periapical periodontitis. assays were done on human whole blood cultures from patients with differing numbers of periapical lesions; those having radiographically clear periapical lesions in 10 or more teeth (high lesion group), in one or two teeth (low lesion group), and healthy volunteers having no peri ...199810023261
elastase activity of anaerobes isolated from amniotic fluid with preterm premature rupture of membranes.a total of 131 anaerobes isolated from amniotic fluid with preterm premature rupture of membranes and stored were examined for elastolytic activity by the method described by williams et al (lett appl microbiol 1988;7:173-6).19999988804
unexpected cross-reaction with fusobacterium necrophorum in a pcr for detection of mycoplasmas.cross-reactions with fusobacterium necrophorum were found in a pcr designed for detection of a wide range of mycoplasma species. twenty-five strains of fusobacterium were examined; all 14 f. necrophorum strains reacted positively, whereas all 7 fusobacterium nucleatum strains reacted negatively. two strains that were not f. necrophorum yielded variable results.19999986867
coronal leakage: endotoxin penetration from mixed bacterial communities through obturated, post-prepared root canals.coronal leakage has been considered as one of the major causes of failure in root canal treatment. bacteria have been shown to penetrate the root canal obturating materials and influence the periapical tissues. endotoxin, a component of gram-negative bacteria, is a potent inflammatory agent and may be able to penetrate obturating materials faster than bacteria. this in vitro study examined the possible penetration of post-prepared canals by endotoxin and bacterial cells derived from mixed bacter ...19989922745
oral microbial flora in sjögren's study presence of microorganisms associated with caries, gingivitis, oral opportunistic infections, and extent of hyposalivation in dentate patients with primary and secondary sjögren's syndrome (ss).19999918250
preparation and characterization of an actinomyces naeslundii aggregation factor that mediates coaggregation with porphyromonas gingivalis.intergeneric coaggregation is responsible for the complexity of the microbiota in human dental plaque and is believed to be important in the initial bacterial colonization of the human oral cavity. actinomyces naeslundii, an early colonizer of the tooth surface, may enhance subsequent colonization by porphyromonas gingivalis which is associated with adult periodontitis. the purpose of this study was to isolate and characterize the a. naeslundii aggregation factor (anaf) that mediates coaggregati ...19989879519
identification of highly fucosylated n-linked oligosaccharides from the human parotid gland.the glycosylation of a number of constituents of human saliva is known to modify its biological roles, such as its lubricating properties and binding of microbial flora. gillece-castro et al. [gillece-castro, b. l., prakobphol, a., burlingame, a. l., leffler, h. & fisher, s. j. (1991) j. biol. chem. 266, 17358-17368] have proposed that the major glycan on the salivary proline-rich glycoproteins is a trifucosylated biantennary sugar with one difucosylated and one unfucosylated antenna. furthermor ...19989874230
analysis of serum antibody responses to periodontopathogens in early-onset periodontitis patients from different geographical locations.serum antibody specificity to oral micro-organisms was used to delineate the pathogens associated with early-onset periodontal diseases in a turkish population. additionally, comparison of the findings to those derived from a clinically similar us patient population described differences in bacterial specific antibody between these 2 geographic regions. serum from 89 (ljp), 86 (rpp) and 94 (normal) subjects was analyzed (elisa) to determine igg antibody to 14 oral micro-organisms. all ljp patien ...19989869349
preterm labor and bacterial intraamniotic infection: arachidonic acid liberation by phospholipase a2 of fusobacterium nucleatum.the studies presented in this report were undertaken to evaluate whether fusobacterium nucleatum, a common anaerobic isolate in intrauterine infection, stimulates arachidonic acid metabolism, a rate-limiting step for prostaglandin synthesis, in the human uterine endometrium.19989855600
septic arthritis due to fusobacterium nucleatum. 19989851282
additional clinical and microbiological effects of amoxicillin and metronidazole after initial periodontal therapy.the aims of this study were to evaluate the clinical and microbiological effects of initial periodontal therapy (it) and to determine the additional effects of systemic amoxicillin (flemoxin solutab) 375 mg tid plus metronidazole 250 mg tid therapy, in patients with adult actinobacillus actinomycetemcomitans (aa)-associated periodontitis in conjunction with either porphyromonas gingivalis (pg), bacteroides forsythus (bf) and/or prevotella intermedia (pi). in addition the adverse effects of the a ...19989846793
[a comparative in-vitro study of the bactericidal efficacy of sodium hypochlorite and chlorhexidine gluconate plus cetrimide on root canal anaerobic bacterial flora].the permanence of necrotic tissues and/or bacteria may be responsible for persistent root canal infection. root canal irrigation plays an important role in the success of endodontic treatment given that, on the one hand, it encourages the progressive elimination of the smear layer and, on the other, it neutralises microbic flora within the canal. the aim of this study was to test root canal irrigants from a microbiological point of view: sodium hypochlorite 5% (niclor ogna, milan) and a new gene ...19989835745
polymicrobial coronal leakage of super eba root-end fillings following two methods of root-end preparation.the aim of this study was to compose in vitro coronal leakage of a super eba root-end filling material after two root-end cavity preparation techniques. a mixed anaerobic microbial marker was used. forty-five extracted human teeth with straight, single root canals were prepared chemo-mechanically to a size 40 master apical file. the teeth were divided into experimental groups (35 teeth) and control groups (10 teeth). forty teeth (35 experimental teeth and five negative control teeth) were obtura ...19989823138
bacterial eradication from root dentine by ultrasonic irrigation with sodium hypochlorite.the study aimed to evaluate intracanal irrigation procedures in eradicating bacteria from surface, shallow and deep layers of root dentine using extracted human teeth. artificial bacterial smear layer was successfully produced by rubbing a mixture of dental plaque and artificially decalcified dentine or carious dentine on root canal walls. the reservoir holes were 3.5 mm in depth, 1 mm in diameter prepared 1.5 mm apart and parallel to the root canals on the decrowned planes, in which five separa ...19989823113
functional changes in thp-1 human monocytic cells after stimulation with lipopolysaccharide of oral microorganisms and granulocyte macrophage colony stimulating factor.a human thp-1 monocyte cell line culture system has been utilized to observe the effect of granulocyte macrophage colony stimulating factor (gm-csf) supplementation with lipopolysaccharide (lps) of oral microorganisms to stimulate monocyte/macrophage functional activity. lps of oral microorganisms, fusobacterium nucleatum and porphyromonas gingivalis was produced by phenol-water extraction and characterized. the phagocytosis assay was performed using f1tc labeled saccharomyces yeast particles. p ...19989805230
microbiological findings in prepubertal periodontitis. a case report.generalized pre-pubertal periodontitis (gpp) is a rare entity that usually affects children with severe systemic diseases. we report the case of a 7-year-old male patient diagnosed with gpp, with no apparent systemic condition, who lost all his primary teeth to periodontal disease. before extractions, while he was still in mixed dentition the subgingival plaque was collected and analyzed using dna probes to 40 different microorganisms. putative periodontopathogens such as prevotella intermedia, ...19989802719
sinusitis in neurologically impaired children.the microbiologic features of infected sinus aspirates in nine children with neurologic impairment were studied. anaerobic bacteria, always mixed with aerobic and facultative bacteria, were isolated in 6 (67%) aspirates and aerobic bacteria only in 3 (33%). there were 24 bacterial isolates, 12 aerobic or facultative and 12 anaerobic. the predominant aerobic isolates were klebsiella pneumoniae, escherichia coli, and staphylococcus aureus (2 each) and proteus mirabilis, pseudomonas aeruginosa, hae ...19989781990
cytotoxic effect of endodontic bacteria on periapical fibroblasts.this study was conducted to investigate the effects of sonicated bacterial extracts (sbes) from anaerobic gram-negative bacteria on periapical fibroblast obtained from the apical portion of human periodontal ligaments. porphyromonas endodontalis, porphyromonas gingivalis, prevotella intermedia, and fusobacterium nucleatum were chosen from among the endodontic bacteria isolated from root canals having a periapical lesion and compared in terms of their cytotoxicity. the purpose of this study was t ...19989759015
identification and analyses of periodontal pathogens in taiwan by microbiological tests.the purpose of this study was to use microbiological tests for diagnosis of periodontal diseases in taiwan. anaerobic culture, direct microscopy, indirect immunofluorescence (if), and biochemical tests were used to examine 336 samples for the specific microorganisms in subgingival plaque. the results indicated that gram-negative species and motile bacteria were less frequently detected, and in lower proportion, in samples from healthy sites. the bacteria found frequently in healthy group were th ...19949747334
role of fusobacterium nucleatum and coaggregation in anaerobe survival in planktonic and biofilm oral microbial communities during aeration.coaggregation is a well-characterized phenomenon by which specific pairs of oral bacteria interact physically. the aim of this study was to examine the patterns of coaggregation between obligately anaerobic and oxygen-tolerant species that coexist in a model oral microbial community. obligate anaerobes other than fusobacterium nucleatum coaggregated only poorly with oxygen-tolerant species. in contrast, f. nucleatum was able to coaggregate not only with both oxygen-tolerant and other obligately ...19989746571
analysis of ph-driven disruption of oral microbial communities in vitro.previously, a mixed culture chemostat system was used to demonstrate that the ph generated from carbohydrate metabolism, rather than carbohydrate availability per se, was responsible for the shifts observed in the oral microflora which are associated with high carbohydrate diets and the development of dental caries. the aim of this study was to determine more accurately the microbially generated ph at which such shifts occurred. nine oral bacteria were grown in three independent chemostats, and ...19989745120
early-onset periodontitis associated with down's syndrome--clinical interventional study.individuals with down's syndrome (ds) have an increased prevalence of periodontal disease compared with otherwise normal, age-matched control groups and other mentally handicapped patients of similar age distribution. the exaggerated immune-inflammatory response of the tissues cannot be explained by poor oral hygiene alone and might be the result of an impaired cell-mediated and humoral immunity and a deficient phagocytic system. as far as the progression and severity of destruction, the oral ma ...19989722720
anaerobic bacteremia and fungemia in patients undergoing endodontic therapy: an overview.oral focal infection, a concept neglected for several decades, is a subject of controversy. recent progress in classification and identification of oral microorganisms has renewed interest in focal infection. the aim of this study was to use phenotypic and genetic methods to trace microorganisms released into the bloodstream during and after endodontic treatment back to their presumed source--the root canal. microbiological samples were taken from the root canals of 26 patients with asymptomatic ...19989722712
preterm birth: associations with genital and possibly oral microflora.opportunistic pathogenic microbes are indigenous to the female lower genital tract and etiologic in many types of pelvic infections and, apparently, a portion of preterm birth (ptb) cases. bacterial vaginosis (bv) is a clinical syndrome based on an altered genital microflora in which gardnerella vaginalis; anaerobic species primarily among prevotella, porphyromonas, bacteroides, peptostreptococcus, and mobiluncus; mycoplasma hominis; and ureaplasma urealyticum become predominant in vaginal secre ...19989722706
oral microbiota in smokers and non-smokers in natural and experimentally-induced gingivitis.the present study primarily aimed at investigating the oral microbiota in smokers and non-smokers with established gingivitis and monitoring its composition during experimental gingivitis. secondly, it aimed at examining whether the composition of the microbiota is associated with different levels of gingival inflammation during this experimental gingivitis trial. for this purpose, 25 non-dental university students with gingivitis were recruited. 11 subjects were smokers and 14 were non-smokers. ...19989722273
subgingival microbial profile of papillon-lefèvre patients assessed by dna-probes.the prevalence of 18 selected bacterial species was assessed by means of "checkerboard" dna-dna hybridisation in a group of 12 saudi-arabian adolescents with papillon-lefèvre syndrome. a total of 36 tooth sites were investigated. the patients exhibited severe periodontal disease with deep pockets. all 12 patients harboured the putative bacterial pathogens p. intermedia, f. nucleatum, p. micros and s. intermedius while t. denticola, b. forsythus, p. nigrescens, e. corrodens, s. noxia and c. rectu ...19989722266
superficial suppurative thrombophlebitis in children, caused by anaerobic bacteria.the purpose of this study was to present the microbiology and clinical features of six children with superficial suppurative thrombophlebitis (sst) caused by anaerobic bacteria.19989722004
in vitro activity of hmr 3647 against anaerobic bacteria.the aim of the present investigation was to determine the in vitro activity of hmr 3647 compared with other antimicrobial agents against anaerobic bacteria. the activity of hmr 3647 was determined against 342 clinical isolates of anaerobic bacteria by the agar dilution method and was compared with azithromycin, clarithromycin, roxithromycin, erythromycin, cefoxitin, imipenem, clindamycin and metronidazole. among the macrolides hmr 3647 and among the beta-lactams imipenem were the most active age ...19989720465
expression of the peptide antibiotic human beta-defensin 1 in cultured gingival epithelial cells and gingival tissue.human beta-defensin-1 (hbd-1) is a member of the family of small cationic antimicrobial peptides that have been identified in several mucosal epithelia. because human gingival epithelium is a site that is constantly challenged by oral microorganisms, we examined the expression of hbd-1 in human gingival epithelial and fibroblast cell cultures and tissue samples. cell cultures were challenged with cell wall extracts of porphyromonas gingivalis or fusobacterium nucleatum, escherichia coli lipopoly ...19989712771
bone resorption caused by three periodontal pathogens in vivo in mice is mediated in part by prostaglandin.gingival inflammation, bacterial infection, alveolar bone destruction, and subsequent tooth loss are characteristic features of periodontal disease, but the precise mechanisms of bone loss are poorly understood. most animal models of the disease require injury to gingival tissues or teeth, and the effects of microorganisms are thus complicated by host responses to tissue destruction. to determine whether three putative periodontal pathogens, porphyromonas gingivalis, campylobacter rectus, and fu ...19989712762
an aminopeptidase nutritionally important to fusobacterium nucleatum.the properties of an aminopeptidase (ap) from fusobacterium nucleatum were studied in view of the fact that this organism, along with other gram-negative anaerobes involved in periodontal diseases, survives in the subgingival environment by obtaining energy via the fermentation of a small number of peptide-derived amino acids. the ap was found to be cell-associated and was isolated from disrupted chemostat-grown cells. it was purified by (nh4)2so4 fractionation, two column chromatographic steps ...19989695913
experimental root canal infections in conventional and germ-free mice.a small animal model was evaluated to study the interrelationships between microorganisms after their implantation in root canals (inferior central incisors) using germ-free (gf) and conventional (cv) mice. the selected microorganisms were: porphyromonas endodontalis (atcc 35406), eubacterium lentum (atcc 25559), peptostreptococcus anaerobius (atcc 27337), fusobacterium nucleatum (atcc 10953), escherichia coli (atcc 25922), and enterococcus faecalis (atcc 4083). only p. anaerobius, e. coli, and ...19989693583
clonal analysis by ribotyping of fusobacterium nucleatum isolates obtained from healthy young adults with optimal plaque control.fusobacterium nucleatum is a gram-negative anaerobic rod implicated in the pathogenesis of periodontal disease. however, this organism has also been frequently identified in high numbers in healthy adults. these observations suggest that the species may comprise different clonal types, some of which may participate in disease. the purpose of the present investigation was to use restriction endonuclease analysis (rea) and ribotyping to characterize f. nucleatum clonal types isolated from healthy ...19989689613
dentilisin activity affects the organization of the outer sheath of treponema denticola.prolyl-phenylalanine-specific serine protease (dentilisin) is a major extracellular protease produced by treponema denticola. the gene, prtp, coding for the protease was recently cloned and sequenced (k. ishihara, t. miura, h. k. kuramitsu, and k. okuda, infect. immun. 64:5178-5186, 1996). in order to determine the role of this protease in the physiology and virulence of t. denticola, a dentilisin-deficient mutant, k1, was constructed following electroporation with a prtp-inactivated dna fragmen ...19989683480
[spinal epidural abscess due to fusobacterium nucleatum].we report a case of spinal epidural abscess. in our knowledge this is the first ever reported case due to fusobacterium nucleatum. an 83 year old female patient with a history of lumbar trauma presented severe lumbalgia. magnetic resonance showed the unusual anterior location, complicated by psoas abscess. laminectomy and lumbar drainage were performed. she was treated with ceftriaxone plus metronidazole during 10 days. the evolution was favourable without any residual neurological morbidity.19979674195
the bacteria of periodontal diseases. 19949673163
severity of localized juvenile periodontitis as related to polymorphonuclear chemotaxis and specific microbial isolates.the purpose of this study was twofold: (a) to determine if an association exists between severity of localized juvenile periodontitis (ljp) and impairment of polymorphonuclear leukocyte (pmn) chemotaxis and/or colonization by specific microbial isolates; (b) to determine if the number of specific microbial isolates, i.e., actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, eikenella corrodens, and campylobacter rectus correlates to clin ...19989663117
subgingival microflora associated with nifedipine-induced gingival overgrowth.the purpose of this study was to examine the composition of subgingival plaque of 140 periodontal lesions in 35 patients with cardiovascular disorders who were administered nifedipine and manifested nifedipine-induced gingival overgrowth (go). age was inversely associated with the go. plaque index and bleeding index showed a significant association with go, while nifedipine dosage and duration of nifedipine therapy were not found to be significant predictors of go. the gingival inflammation as e ...19989660335
the effect of herbal extracts in an experimental mouthrinse on established plaque and gingivitis.the purpose of the present study was to establish in vitro the inhibiting effect of a herbal extract mixture on a selected number of micro-organisms and to test in vivo the effect of a mouthwash containing 6.3 mg/ml herbal extract mixture on plaque and gingivitis as compared to a minus active control mouthrinse. the herbal extract was a mixture of: juniperus communis (juniper), urtica dioca (nettle), achillaea millefolium (yarrow); 1:1:1. in the study, in-vitro, the effect of pure herbal extract ...19989650877
development of an anaerobic bacterial leakage model.the majority of bacteria associated with infections of endodontic origin are strict anaerobes. the purpose of this study was to develop an endodontic microleakage model using strict anaerobic bacteria in a two-chamber system. nine species of anaerobic bacteria were tested for viability and detection by either turbidity or color change of the broth. a survey of ph chromogenic substrates revealed that bromcresol purple (ph 5.2 = yellow, ph 6.8 = purple) could be used as a chromogenic indicator to ...19989641124
studies on fusobacteria associated with periodontal diseases.the physiological and metabolic characteristics of representative isolates of the various subspecies of fusobacterium nucleatum were investigated by growing them in continuous culture in chemically-defined, media. behaving almost identically, these organisms were found to obtain energy from the fermentation of simple carbohydrates such as glucose or fructose or from the fermentation of certain amino acids, free or in the form of small peptides. the latter can be attacked by aminopeptidase activi ...19989612984
granulocyte-macrophage colony-stimulating factor amplification of interleukin-1beta and tumor necrosis factor alpha production in thp-1 human monocytic cells stimulated with lipopolysaccharide of oral microorganisms.cytokines, including granulocyte-macrophage colony-stimulating factor (gm-csf), are used to assist in bone marrow recovery during cancer chemotherapy. interleukin-1beta (il-1beta) and tumor necrosis factor alpha (tnf-alpha) play important roles in inflammatory processes, including exacerbation of periodontal diseases, one of the most common complications in patients who undergo this therapy. a human monocyte cell line (thp-1) was utilized to investigate il-1beta and tnf-alpha production followin ...19989605989
volatile fatty acid, metabolic by-product of periodontopathic bacteria, induces apoptosis in wehi 231 and raji b lymphoma cells and splenic b cells.the ability of butyric acid, an extracellular metabolite from periodontopathic bacteria, to induce apoptosis in murine wehi 231 cells, splenic b cells, and human raji cells was examined. the culture filtrate of porphyromonas gingivalis, prevotella loescheii, and fusobacterium nucleatum, which contains high a percentage of butyric acid, induced dna fragmentation in wehi 231 cells. volatile fatty acid, especially butyric acid, significantly suppressed b-cell viability in a concentration-dependent ...19989596720
activities of hmr 3004 (ru 64004) and hmr 3647 (ru 66647) compared to those of erythromycin, azithromycin, clarithromycin, roxithromycin, and eight other antimicrobial agents against unusual aerobic and anaerobic human and animal bite pathogens isolated from skin and soft tissue infections in humans.the activities of hmr 3004 and hmr 3647 and comparator agents, especially macrolides, were determined by the agar dilution method against 262 aerobic and 120 anaerobic strains isolated from skin and soft tissue infections associated with human and animal bite wounds. hmr 3004 and hmr 3647 were active against almost all aerobic and fastidious facultative isolates (mic at which 90% of the isolates are inhibited [mic90], < or = 0.5 and 1 microg/ml, respectively) and against all anaerobes [bacteroid ...19989593139
in-vitro antibacterial effects of extracts of nigerian tooth-cleaning sticks on periodontopathic bacteria.aqueous extracts from 8 plants used for tooth-cleaning in nigeria were tested for their ability to inhibit the growth of five periodontopathic bacteria, porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, eikenella corrodens and campylobacter rectus. extracts of all the plants except that of massularia acuminata exhibited varying growth inhibitory potentials on the microorganisms. extract of terminalia glaucescens showed the widest spectrum of activity, inhibiting the growt ...19959590895
the use of a 16s rdna directed pcr for the detection of endodontopathogenic bacteria.the study evaluates a 16s rdna directed polymerase chain reaction (pcr) to detect and differentiate bacteria in necrotic root canal samples. the examination focused on species that are fastidious concerning culture or are difficult to differentiate after culturing by biochemical methods. in the described pcr assay, a universal 16s rdna directed forward primer in combination with a highly specific reversed one was used to amplify taxon specific gene fragments of 230 to 950 bp length. a similar pc ...19979587296
trovafloxacin compared with levofloxacin, ofloxacin, ciprofloxacin, azithromycin and clarithromycin against unusual aerobic and anaerobic human and animal bite-wound pathogens.the activity of trovafloxacin and five other oral agents against 250 aerobic and 137 anaerobic strains isolated from human and animal bite wounds was determined by an agar dilution method. trovafloxacin was active against all aerobic and fastidious facultative isolates at < or = 0.5 mg/l and all anaerobes at < or = 2 mg/l (bacteroides tectum, porphyromonas salivosa and prevotella heparinolytica, < or = 0.25 mg/l; porphyromonas spp., < or = 0.5 mg/l; prevotella spp. and peptostreptococci, < or = ...19989578167
helicobacter pylori adheres selectively to fusobacterium spp.helicobacter pylori strains atcc 43504 and atcc 43629 were tested for their ability to coaggregate with 79 strains of bacteria representing 16 genera. all except two of the strains were of human origin, and most of the strains were isolated from the oral cavity. the helicobacters failed to coaggregate with all strains except the fusobacteria. several coaggregations were partially or completely inhibited by lactose. strong coaggregation was seen with each of four subspecies of fusobacterium nucle ...19989573823
characterization of a novel n-acetylneuraminic acid-specific fusobacterium nucleatum pk1594 adhesin.fusobacterium nucleatum has been identified as significantly associated with sites with active periodontal disease and, as a group, the oral fusobacteria coaggregate with members of all oral bacteria genera tested. monoclonal antibodies were prepared and used in conjunction with other potential inhibitors, such as simple sugars and amino acids, to characterize coaggregation interactions, of f. nucleatum pk1594. four unique monoclonal antibodies, 5h11, 14c7, 19f2 and 29c12, were obtained by their ...19989573822
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