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impaired bactericidal activity of pmn from two brothers with necrotizing ulcerative gingivo-periodontitis.the purpose of this study was to investigate the pathogenesis of necrotizing ulcerative gingivo-periodontitis (anup) diagnosed in two brothers, age 9 (anup1) and 14 (anup2) from rural egypt. complete blood count, differential and blood chemistry were within normal limits for both brothers and they were not malnourished. the phagocytosis and killing function of their polymorphonuclear leukocytes (pmn) towards four bacterial species were assessed using a fluorochrome microassay. the selection of b ...19948195981
bacteriological diagnosis of periodontal disease.dental plaque bacteria cause virtually all the forms of inflammatory periodontal disease. periodontitis is caused by the specific periodontopathic bacteria, which induce destruction of connective tissue attachment and adjacent alveolar bone. examinations to identify the infections by porphyromonas gingivalis, actinobacillus actinomycetemcomitans, prevotella intermedia, campylobacter rectus, bacteroides forsythus, fusobacterium nucleatum, eikenella corrodens and treponema denticola have recently ...19948620588
similarities between fusobacterium nucleatum and bacteroides fragilis studied by two dna probes derived from fusobacterium nucleatum.a polymerase chain reaction (pcr)-amplified oligonucleotide dna probe corresponding to a fusobacterium nucleatum fevl dna region coding for a 40-kda major outer-membrane protein (omp) and a randomly cloned 2.1 kb dna probe were found to recognize dna from the gram-negative bacteria fusobacterium nucleatum and bacteroides fragilis on southern blots and slot blots. the results indicate sequence similarity within the dna fragments studied. immunoblots tested with polyclonal antibodies against whole ...19948153579
relationship between salivary blood group antigens, microbial flora and periodontal condition in young adults.to investigate a possible rôle for salivary blood-group antigens in the relative frequencies of selected periodontal pathogens and commensal oral micro-organisms, we studied the clinical and microbiological condition in young adults with or without blood group reactive substances in saliva (secretors or non-secretors respectively). clinical measurements were recorded at the ramfjord teeth in 81 1st-year dental students. in addition, presence of interproximal loss of attachment (la) was assessed ...19948157769
adherence of oral microorganisms to guided tissue membranes: an in vitro study.microorganisms can adhere and colonize on an exposed guided tissue regeneration (gtr) membrane thus developing a nidus of infection. the purpose of this study was to compare early bacterial adhesion to three different gtr membranes. expanded polytetrafluoroethylene, polyglactin 910, and collagen were used as the test membranes. in part i of this study 15 different oral microbes were used to compare their relative ability to adhere to the membranes. six of the most strongly adherent bacteria (act ...19948164114
bacterial vaginosis and vaginal microorganisms in idiopathic premature labor and association with pregnancy outcome.the vaginal microflora of 49 women in idiopathic preterm labor was compared with that of 38 term controls to determine whether the presence of bacterial vaginosis (bv) and/or specific microorganisms would influence the rate of preterm delivery. demographic factors, pregnancy outcome, and reproductive history were also studied. bv, as defined by the presence of clue cells in a vaginal wet mount and characteristic microbial findings in a stained vaginal smear and vaginal culture, was more common i ...19948126176
detection of porphyromonas gingivalis in gingival exudate by a dipeptide-enhanced trypsin-like activity.porphyromonas gingivalis in subgingival plaque is an important risk factor for future periodontal attachment loss in susceptible adults. the elimination of p. gingivalis is usually concomitant with a healing process. therefore, it should be valuable to have an easy chairside method to follow the effect of periodontal treatment on p. gingivalis detection as well as on its eventual reappearance during the maintenance period. we have previously reported the stimulation of amidolytic activity of p. ...19948133415
modeling the relationship between clinical, microbiologic, and immunologic parameters and alveolar bone levels in an elderly population.a cross-sectional periodontal study of 74 subjects aged 65 to 75 years was performed. clinical data were collected and related to microbiological and immunological data. a statistical model (step-wise multiple regression) of factors related to bone loss was created initially using clinical data only; then by adding either the microbiologic or immunologic data; and then by using clinical, microbiologic, and immunologic data together. when only clinical data were considered, three factors were fou ...19948133417
microbiology of tonsillar surfaces in infectious mononucleosis.to compare the cultures of tonsillar surface aerobic and anaerobic bacterial flora taken during the acute phase of infectious mononucleosis with the repeated cultures taken 2 months later.19948118535
cytotoxicity in bacterial cultures: interaction and cell-specificity, possible factors in periodontal disease.cytotoxicity in culture media of various growing bacterial strains was estimated by cr-51 release of labelled target-cells. interaction studies were made by adding each of the different uv-killed bacteria to the medium with viable bacteria. the reference oral bacterial strains were: actinobacillus actinomycetemcomitans y4, porphyromonas gingivalis, fusobacterium nucleatum and streptococcus mitis, which were compared with the reference bacteria staphylococcus aureus 209 and staphylococcus epiderm ...19947799211
utilization of glutathione (l-gamma-glutamyl-l-cysteinylglycine) by fusobacterium nucleatum subspecies nucleatum.although fusobacteria use amino acids and peptides as energy source, it is not known whether they are able to actively transport peptides into the cell. in the present study the tripeptide glutathione was used as a model substance to investigate peptide uptake in fusobacterium nucleatum subsp. nucleatum. cells harvested after 2 days of growth on blood agar or in their exponential growth phase in broth were suspended in buffer with glutathione, l-cysteinylglycine and l-cysteine. as a measure of c ...19947808772
bacterial interference in vitro. comparison between a quantitative kinetic and a cocultivation blood agar test method.the aim of the present study was to compare two methods for estimation of bacterial growth interference between various bacteria using a bioscreen robot analyzer, allowing kinetic documentation, and a cocultivation test on blood agar plates. six laboratory strains with different virulence and growth requirements were used: actinobacillus actinomycetemcomitans, porphyromonas gingivalis, fusobacterium nucleatum, streptococcus mitis, staphylococcus aureus, and staphylococcus epidermidis. the interf ...19947833000
in vitro activity of du-6859a against anaerobic bacteria.the activity of a new quinolone agent, du-6859a, against 330 strains of anaerobic bacteria was determined by using the national committee for clinical laboratory standards-approved wadsworth brucella laked blood agar method; the activity of du-6859a was compared with those of amoxicillin-clavulanate (2:1), chloramphenicol, ciprofloxacin, clindamycin, fleroxacin, imipenem, lomefloxacin, metronidazole, sparfloxacin, and temafloxacin. du-6859a and chloramphenicol inhibited all of the isolates at co ...19947840599
orogenital contact: a cause of chorioamnionitis?fusobacterium nucleatum and capnocytophaga species are common oral pathogens and infrequent causes of systemic infection in patients with compromised immunity or disrupted mucosal integrity. the isolation of both organisms from a clinical specimen suggests an oral source of infection.19949205437
the bacteria of periodontal diseases. 19949673163
identification and analyses of periodontal pathogens in taiwan by microbiological tests.the purpose of this study was to use microbiological tests for diagnosis of periodontal diseases in taiwan. anaerobic culture, direct microscopy, indirect immunofluorescence (if), and biochemical tests were used to examine 336 samples for the specific microorganisms in subgingival plaque. the results indicated that gram-negative species and motile bacteria were less frequently detected, and in lower proportion, in samples from healthy sites. the bacteria found frequently in healthy group were th ...19949747334
demonstration of human immunoglobulin g fc-binding activity in oral bacteria.nonimmune binding of immunoglobulins via the fc fragment may reduce opsonization and phagocytosis of bacteria and is thus considered a virulence factor. the aim of this study was to investigate a wide range of oral bacterial strains for the presence of human immunoglobulin g (igg) fc-binding activity. a total of 132 strains representing 40 different gram-positive and gram negative bacterial species were tested for igg fc-binding activity by using a fast and simple dot blot procedure with horsera ...19947496956
maturation of b lymphocytes. i. concurrent appearance of increasing ig, ia, and mitogen responsiveness. 1994368238
[experience in echography in the early differential diagnosis of tubal pregnancy]. 1994262991
[independence of the increase in the number of action potentials and excitability in the response of mollusk neurons during their combined activation]. 19943250841
systematic approach to diagnosis and coding of pediatric cardiac disease.a new system for coding congenital heart defects and related problems was developed to facilitate the computerized storage and retrieval of detailed diagnostic information. the system is based on the segmental approach to diagnosis with comprehensive tables indicating all possible mutually exclusive intersegmental alignments and connections. diagnostic entities rather than nomenclature are the subjects of the codes. this system represents the best effort to date to develop an all-inclusive, prec ...19953534806
in-vitro antibacterial effects of extracts of nigerian tooth-cleaning sticks on periodontopathic bacteria.aqueous extracts from 8 plants used for tooth-cleaning in nigeria were tested for their ability to inhibit the growth of five periodontopathic bacteria, porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, eikenella corrodens and campylobacter rectus. extracts of all the plants except that of massularia acuminata exhibited varying growth inhibitory potentials on the microorganisms. extract of terminalia glaucescens showed the widest spectrum of activity, inhibiting the growt ...19959590895
inhibition of neutrophil and monocyte defensive functions by nicotine.to learn more about the effects of smokeless tobacco on the defensive functions of neutrophils, we studied the influence of nicotine on these cells in vitro, looking at their bactericidal activity against oral pathogens, and at their ability to produce microbicidal reactive oxygen species (oxygen radicals). exposure of human blood neutrophils to nicotine (0.01% to 0.1%) inhibited their ability to kill actinomyces naeslundii, actinobacillus actinomycetemcomitans, and fusobacterium nucleatum. alth ...19958683417
cytology of pulmonary fusobacterium nucleatum infection. a case report.a patient with pulmonary fusobacterium nucleatum infection presenting as a solitary nodule is reported. the infectious nature of the lung nodule was disclosed by ultrasound-guided percutaneous transthoracic fine needle aspiration cytology (fnac) with liu's stain. the cytologic characteristics of f nucleatum infection are described. the usefulness of real-time ultrasound in fine needle aspiration diagnosis of a peripheral lung lesion is demonstrated, and the role of liu's stain in fnac is emphasi ...19957846999
antimicrobial susceptibility of periodontopathic bacteria associated with failing implants.the aim of this study was to examine the subgingival microflora associated with failing implants, and to determine their susceptibility to commonly used antibiotics in periodontal therapy and dental practice. thirteen partially edentulous patients with 19 failing implants were selected. clinical examination included probing depth, attachment level, gingival index, plaque index, and radiographic analyses. two subgingival plaque samples were taken from each failing implant and analyzed for microbi ...19957891253
endodontic pathogens: propagation of infection through patent dentinal tubules in traumatized monkey teeth.periapical pathology indicating endodontic infection, when present in marginal periodontitis-affected teeth, has recently been shown to be an aggravating factor in progression of marginal destruction. this has been associated with patency of dentinal tubules in the tooth cervix, an area normally devoid of cementum following periodontal therapy. these studies are, however hampered by that only circumstantial evidence such as presence of periapical destruction have been applied as criteria of endo ...19958625937
stable-isotope studies of glutamate catabolism in fusobacterium nucleatum.catabolism of glutamate was investigated in fusobacterium nucleatum, an anaerobic micro-organism that is strongly implicated in periodontal disease. the distribution of labels in acetate and butyrate derived from [13c]glutamates was determined by nmr spectroscopy and ms. the label from l-[5-13c]glutamate was not incorporated, whereas c-1 of acetate and butyrate were efficiently labelled by l-[1-13c]glutamate; these results indicated that the hydroxyglutarate pathway predominated. in butyrate, en ...19958573293
endodontic pathogens in periodontal disease augmentation.periapical pathology indicating endodontic infection, when present in periodontitis-affected teeth, has recently been shown to be correlated to marginal periodontal breakdown. this has been associated with patency of dentinal tubules in the tooth cervix, an area normally devoid of cementum following periodontal therapy. these studies are, however hampered by that only circumstantial evidence such as presence of periapical destruction have been applied as criteria of endodontic infection. the aim ...19958583015
oral microbiota in subjects with a weak or strong response in experimental gingivitis.the purpose of the present study was to examine the composition of the oral microbiota in subjects who had previously demonstrated to develop either a weak or strong response to experimental gingivitis. for this study, subjects were selected from a pool of 25 individuals who had participated twice in an experimental gingivitis trial. out of these 25 panellists, 6 subjects were selected who had developed 2x a weak gingival inflammatory response and 7 subjects who had developed 2x a strong gingiva ...19958583022
[subgingival distribution of periodontopathic bacteria in periodontic patients and susceptibility of these bacteria to minocycline-hcl].the present study was carried out to examine the distribution of six periodontopathic bacteria in deep periodontal pockets and to reconfirm the effect of periocline on these periodontopathic bacteria. samples from sixty-two periodontal pockets were collected at pocket depths of over 4 mm in twenty-one periodontitis patients aged 43 to 75 years. after sampling, periocline was applied topically to the selected pockets once a week for four weeks and reexamined. the detected rates of the periodontop ...19958583161
molecular characterization of a 40-kda outer membrane protein, foma, of fusobacterium periodonticum and comparison with fusobacterium nucleatum.the 40 kda-outer membrane protein foma of fusobacterium periodonticum atcc 33693 was found to exhibit heat modifiable properties, typical for a porin, and n-terminal sequencing indicated a close relationship to the porin foma of fusobacterium nucleatum. a polymerase chain reaction approach was therefore applied for sequencing the foma gene of f. periodonticum, and nucleotide and deduced amino acid sequences were aligned and compared with the corresponding sequences of different strains of f. nuc ...19958596666
effect of sonicated material from fusobacterium nucleatum on the functional capacity of accessory cells derived from dental pulp.fusobacterium nucleatum-derived components were studied for their effect on the ability of accessory pulpal cells to induce t-lymphocyte proliferation. in initial experiments, spleen cells were used to establish concentration ranges at which testing of the effect of the bacterial components would be suitable. at low concentrations, bacterial substances induced a stimulatory effect on the proliferation rate of spleen cells, but not when purified t-lymphocytes were incubated in the presence of con ...19958602332
insertional inactivation of binding determinants of streptococcus crista cc5a using tn916.intermicrobial binding plays an important role in the ecology of the oral cavity because it represents one mechanism by which specific bacteria colonize dental plaque. the formation of "corncobs", a morphologically distinct microbial unit composed of streptococcus crista and fusobacterium nucleatum, is a highly specific binding interaction that depends on the presence of polar tufts of fimbriae on the streptococci. we have used a genetic approach to examine the role of streptococcal cell surface ...19958602334
cell envelope and cell wall immunization of macaca fascicularis: effect on the progression of ligature-induced periodontitis.the nonhuman primate, macaca fascicularis, was used to study the role of immunization with selected members of the periodontopathic microbiota in the longitudinal progression of ligature-induced periodontitis. animals were immunized with cell envelope antigens prepared from porphyromonas gingivalis and prevotella intermedia, and a mixture prepared from fusobacterium nucleatum, campylobacter rectus, and actinomyces viscosus. serum immunoglobulin g (igg), igm and iga isotype antibodies increased s ...19958602339
viability of four putative periodontal pathogens and enteric rods in the anaerobic transport medium vmga iii.the aim of this study was to determine the survival in vmga iii of porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, actinobacillus actinomycetemcomitans and enteric rods in laboratory cultures as well as in subgingival plaque samples. laboratory strains of the 4 putative periodontal pathogens and escherichia coli were used in the laboratory part of this study. also, 31 subgingival plaque samples were obtained from 22 periodontal patients and stored in vmga iii. each samp ...19958602345
comparative virulence of periodontopathogens in a mouse abscess model.this report compares the virulence of selected strains of p. gingivalis, a. actinomycetemcomitans, c. rectus, f. nucleatum and t. denticola in a murine model as a measure of pathogenic potential of these oral microorganisms. the characteristics of the tissue destruction associated with these monoinfections were then related to a potential model for bacterial synergism in progressing periodontitis.19958705817
comparison of host tissue and bacterial dipeptidyl peptidases in human gingival crevicular fluid by analytical isoelectric focusing.earlier work has shown that gingival crevicular fluid (gcf) contains dipeptidyl peptidase (dpp) activities that resemble those in host tissue. here, further comparisons were made with enzymes from suspected periodontal pathogens. gingival tissue and gcf were collected from patients with chronic periodontitis. dpp ii and dpp iv fractions with acid and alkaline ph optima, respectively, were separated from crude tissue extracts by gel-filtration chromatography. bacterial cell sonicates were prepare ...19957487574
evaluation of periodontal treatments using controlled-release tetracycline fibers: microbiological response.in a 12-month multi-center study of 116 adult periodontitis subjects, six putative periodontal pathogens were monitored by dna probe methods in a subset of 31 subjects. monitored species included porphyromonas gingivalis (pg), prevotella intermedia (pi), fusobacterium nucleatum (fn), eikenella corrodens (ec), campylobacter rectus (cr), and actinobacillus actinomycetemcomitans (aa) with an average detection limit of 1.8 x 10(4) bacterial colony forming units/sample. the microbiological response t ...19957473013
peptide mapping and amino acid sequence analysis of the 40 kda outer membrane protein of fusobacterium nucleatum. 19957502770
cellular adhesion molecules on periodontal lymphocytes.t cell induced differentiation of b cells has been shown to be dependent on the cd2/lfa-3 and lfa-1/icam-1 pathways. flow cytometric analysis was used to examine these adhesion molecules on t and b cells extracted from gingival tissues before and after stimulation with the putative periodontopathic bacteria, porphyromonas gingivalis and fusobacterium nucleatum. adhesion molecule expression on peripheral blood cells from healthy adults was used as a control. approximately 50 per cent of b cells e ...19957540389
immune response to fusobacterium nucleatum and prevotella intermedia in patients with peritonsillar cellulitis and abscess. 19957548558
a retrospective review of cases of anaerobic empyema and update of bacteriology.we conducted a retrospective study to update the bacteriology of 46 cases of anaerobic empyema that were originally studied between 1976 and 1993 at the wadsworth anaerobic bacteriology clinical research laboratory (los angeles). anaerobic bacteriologic studies were completed for all 46 pleural fluid specimens, and aerobic bacteriologic studies were completed for 41 of these specimens. thirty-seven clinical charts were available for review. a total of 161 anaerobic isolates (3.5 per patient) rep ...19957548560
bacteriology of skin and soft-tissue infections: comparison of infections in intravenous drug users and individuals with no history of intravenous drug use.the bacteriology of cutaneous or subcutaneous abscesses (86 specimens) among intravenous drug users (ivdus) was compared with the bacteriology of abscesses (74 specimens) in patients with no history of intravenous drug use (non-ivdus). the ivdu abscesses yielded 173 aerobes and 131 anaerobes. staphylococcus aureus was the most common aerobe isolated (50% of specimens yielded this isolate), followed by "streptococcus milleri" (46%). the commonly encountered anaerobes were fusobacterium nucleatum ...19957548575
microbiological study of juvenile periodontitis in chile. 19957548581
induction of beta-lactamase production in the anaerobic microflora by cefoxitin. 19957548595
oligonucleotide probes to the 16s ribosomal rna: implications of sequence homology and secondary structure with particular reference to the oral species prevotella intermedia and prevotella nigrescens.eight oligonucleotides based upon regions of the small subunit 16s ribosomal rna gene sequences were analysed against a background of their position within the molecule and their two-dimensional structure to rationalise their use in recognising prevotella intermedia and prevotella nigrescens. the 41 clinical isolates from both oral and respiratory sites and two reference strains were subjected to dna-dna hybridisation and multilocus enzyme electrophoresis to confirm their identity. alignment of ...19957553378
some suspected periodontopathogens and serum antibody response in adult long-duration insulin-dependent diabetics.the subgingival microflora and serum antibody response were examined in long-duration insulin-dependent diabetics and age- and sex-matched non-diabetics. the material consisted of 9 diabetics aged 40-49 years and 19 aged 50-59 years, 13 non-diabetics aged 40-49 years and 21 aged 50-59 years. the bacterial species studied (actinobacillus actinomycetemcomitans, campylobacter rectus, capnocytophaga spp, eikenella corrodens, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia) w ...19957560223
effect of periodontal treatments on serum igg antibody titers against periodontopathic bacteria.serum igg antibody titers to 7 periodontopathic bacteria in periodontitis patients were measured at the 1st visit and after various periodontal treatments with clinically successful improvement, in order to evaluate what kind of factors are associated with changes of serum antibody titers. 20 patients (10 male and 10 female from 23 to 61 years old) with adult, rapidly progressive periodontitis were enrolled in this study. all patients received initial preparation and most of them also underwent ...19957560233
a 6-month home-usage trial of 0.1% and 0.2% delmopinol mouthwashes (ii). effects on the plaque microflora.the effects of 0.1% and 0.2% delmopinol mouthwashes on supragingival plaque flora were investigated in a 6-month home-use study. 141 subjects were studied from whom plaque was collected at baseline, 12, 24, and 36 weeks. overall, there were no consistent effects on microscopic or total counts. however, there was a significant reduction in the proportion of dextran-producing streptococci in the active groups compared to the control group throughout treatment. there was no colonisation by candida ...19957560235
volatile fatty acids, metabolic by-products of periodontopathic bacteria, inhibit lymphocyte proliferation and cytokine production.short-chain fatty acids are a major by-product of anaerobic metabolism and can be detected in gingival fluid from periodontal pockets. since most t cells are present subjacent to the pocket epithelium in conjunction with the plasma cells, it is important to know how these t cells are affected by short-chain fatty acids produced by subgingival plaque. the purpose of this study is to examine the effects of extracellular metabolites from periodontopathic bacteria on the proliferation and cytokine p ...19957560387
effects of lipopolysaccharides on human dental pulp cells.human dental pulp cells were treated with 1, 10, and 100 micrograms/ml of lipopolysaccharide (lps). the effects of treatment were examined by measurement of the dna content, protein content, and alkaline phosphatase activity of the cells. lps samples were purified from porphyromonas gingivalis, porphyromonas endodontalis, and fusobacterium nucleatum isolated from root canals, and escherichia coli 0111:b4 lps was used as a positive control. at a concentration of 1 microgram/ml, none of the lpss c ...19957561654
characteristics of multimodal co-aggregation between fusobacterium nucleatum and streptococci.the co-aggregation characteristics between fusobacterium nucleatum and streptococci were examined to clarify the adherence factors participating in the co-aggregation. nineteen strains of f. nucleatum were classified into 8 groups according to co-aggregation titer and inhibition by l-arginine, l-lysine and n-acetyl-d-galactosamine (or lactose). the inhibition activity was, however, very different from strain to strain. with two fusobacterial strains, two inhibitors, which were both inhibition ne ...19957562321
effect of whole oral bacteria and extracted lipopolysaccharides on peripheral blood leukocyte interleukin-2 receptor expression.expression of the interleukin-2 receptor (il-2r) on t cells is the molecular mechanism that initiates the g0 to g1 transition and is the critical first step for t cell proliferation in response to antigen. the effect of whole periodontal bacteria and lipopolysaccharides (lps) on peripheral blood mononuclear cell (pbmc) il-2r expression was examined in vitro. lps induced a modest but significant increase in high affinity il-2r alpha/beta (p55/p75 positive) expression on pbmc over untreated cells ...19957562323
occurrence of certain bacterial species and morphotypes in juvenile periodontitis in chile.the occurrence of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, eikenella corrodens, fusobacterium nucleatum, campylobacter rectus, capnocytophaga species, and certain bacterial morphotypes was determined in 18 affected and 18 unaffected sites in 10 localized juvenile periodontitis (ljp) patients, and in 10 affected and 10 unaffected sites in 5 generalized juvenile periodontitis (gjp) patients. the subgingival proportion of the 7 bacterial species was det ...19957562347
heat shock proteins in the human periodontal disease process.the production of hsp by periodontopathic gram-negative bacteria was examined by sds-page, two dimensional gel electrophoresis, and western blotting using monoclonal antibodies against hsps. strains of actinobacillus actinomycetemcomitans, eikenella corrodens, fusobacterium nucleatum, prevotella intermedia, prevotella nigrescens, prevotella melaninogenica, and treponema socranskii species produced hsp which reacted with anti-yersinia enterocolitica hsp 60 and/or mycobacterial 65-kda hsp monoclon ...19957565172
molecular and immunological characterization of a 64-kda protein of actinobacillus actinomycetemcomitans.the 64-kda protein to which about half the sera from patients with localized juvenile periodontitis and rapidly progressive periodontitis reacted strongly was purified from actinobacillus actinomycetemcomitans y4. determination of the n-terminal sequence of the protein revealed that it was a groel-like protein. the dna fragment containing the groel gene of a. actinomycetemcomitans was amplified by polymerase chain reaction, and the groesl operon was cloned by using colony hybridization with the ...19957567064
antimicrobial activity of cefotaxime tested against infrequently isolated pathogenic species (unusual pathogens).the cefotaxime sodium spectrum of activity is very broad and includes many common species and a variety of less frequently isolated pathogens. we have reviewed the clinical microbiology literature (44 references) and the data base of the university of iowa hospitals and clinics (iowa city, ia) to collect data on the activity of cefotaxime against the less common species. cefotaxime was consistently active against actinobacillus actinomycetemcomitans, capnocytophaga spp., eikenella corrodens, ery ...19957587049
the fusobacterium nucleatum major outer-membrane protein (foma) forms trimeric, water-filled channels in lipid bilayer membranes.the pore-forming activity of the major outer-membrane protein foma of the anaerobic fusobacterium nucleatum was studied in artificial lipid bilayer membranes. foma was isolated from f. nucleatum strains fev1, atcc 10953, and atcc 25586 by extraction with lithium dodecyl sulfate and lithium chloride and had an apparent molecular mass of about 40 kda. when solubilized at low temperatures, the protein ran with an apparent molecular mass of about 62 kda on sds/page. cross-linking experiments and two ...19957588760
fusobacterium nucleatum inhibits human t-cell activation by arresting cells in the mid-g1 phase of the cell cycle.fusobacterium nucleatum has been implicated in the pathogenesis of several diseases, including urinary tract infections, bacteremia, pericarditis, otitis media, and disorders of the oral cavity such as pulpal infections, alveolar bone abscesses, and periodontal disease. we have previously demonstrated that sonic extracts of f. nucleatum fdc 364 were capable of inhibiting human t-cell responses to mitogens and antigens. in this study, we have further characterized this immunosuppressive protein ( ...19957591143
cellular source, activation and inhibition of dental plaque collagenase.dental plaque is the major aetiological factor in periodontal diseases and contains several proteolytic enzymes. the origin of these proteinases is, however, poorly studied. this study was undertaken to characterize collagenase present in dental plaque of adult periodontitis patients. vertebrate-type rather than bacterial-derived collagenase activity was detected in extracts of both supra- and subgingival dental plaque extracts of adult periodontitis patients. dental plaque collagenase was found ...19957593702
antibacterial effects of some root end filling materials.in addition to having good sealing ability, root end filling materials should "ideally" have some antibacterial activity to prevent bacterial growth. this investigation compared the antibacterial effects of amalgam, zinc oxide-eugenol, super eba and a mineral trioxide aggregate on nine facultative bacteria streptococcus fecalis, streptococcus mitis, streptococcus mutans, streptococcus salivarius, lactobacillus species, staphylococcus aureus, staphylococcus epidermidis, bacillus subtilis, and esc ...19957595152
biological activities of lipopolysaccharides from oral bacteria and their relevance to the pathogenesis of chronic periodontitis.chronic periodontitis is a major cause of tooth loss in adults and is a consequence of the colonisation of the subgingival region by organisms such as porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum. lipopolysaccharide (lps) is a constituent of the cell walls of all of these bacteria and is found in large quantities on the surfaces of periodontally-diseased teeth. lps from oral bacteria has a marked effect on most types of cell found in the periodontal tissues includi ...19957597416
mobile genetic elements of fusobacterium nucleatum.the gram-negative anaerobic bacterium, fusobacterium nucleatum, is a predominant member of the human oral flora. as a major component of subgingival plaque, this bacterium has a significant impact on the ecology of the oral cavity due to its ability to adhere to many different microbial species. the objective of this study was to identify and characterize plasmids and transposons that may have the potential to be developed into tools for cloning, genetic transformation, and mutagenesis of oral i ...19957753905
spontaneous gingival antibody production to fusobacterium nucleatum outer membrane in patients with adult periodontitis.the local antibody response to fusobacterium nucleatum outer membrane (fnom) was analyzed in patients with adult periodontitis (ap) at the single cell level. furthermore, we analyzed whether periodontal hygienic treatment could alter the antibody response. the number of igg- and igm-producing cells were investigated in gingival samples collected from 20 patients with ap. the patients were divided into 2 groups, before (bt, n = 9) and after (at, n = 11) periodontal hygienic treatment. four health ...19957767709
succession of putative peri-implant pathogens after root-form and plate-form implant placement in partially dentate adult monkeys.this report describes the succession of putative peri-implant pathogens in partially dentate monkeys after dental implantation and prosthetic reconstruction. tooth and implant (6 root-end form, 4 blade-vent implants) sites in eight monkeys were monitored microbiologically and clinically during the pre-implant stage, abutment connection stage, bridge placement stage, and three and six months after the bridge placement stage. tooth and implant sites were cleaned monthly post-extraction. microbiolo ...19957776158
attachment of fusobacterium nucleatum to fibronectin immobilized on gingival epithelial cells or glass coverslips.to learn more about colonization of the oral epithelium by fusobacterium nucleatum and the role of fibronectin in mediating adhesion of this microorganism, we studied attachment of this bacterium to cultured gingival epithelial cells that were coated with exogenous, purified plasma fibronectin. the three strains of f. nucleatum studied adhered in large numbers to epithelial cells that had been coated with fibronectin, compared with buffer-coated control cells. bacterial adherence was also enhanc ...19957782983
fusobacterium nucleatum, a new invasive pathogen in neutropenic patients?three chemotherapy-induced neutropenic hematologic patients with severe systemic infection caused by fusobacterium nucleatum, a gram-negative anaerobic rod, are described. anaerobic infections are not very common in this patient category, but in a short period of time, several such patients were seen. the infection was considered to be caused by a combination of chemotherapy-induced mucositis, which served as a portal of entry for the systemic infection, and the antibiotic regime used in these p ...19957784823
quantifying the strength of bacterial adhesive interactions with salivary glycoproteins.we adapted an assay that has been used to estimate the strength of eukaryotic cell-cell and cell-extracellular matrix adhesive interactions (mcclay et al., 1981) to quantify the strength of bacterial (streptococci, fusobacteria, actinomyces) interactions with salivary receptors. bacteria are centrifuged onto human submandibular/sublingual or parotid-saliva-coated microtiter wells. plates are sealed with pressure-sensitive, double-sided tape which allows them to be inverted and centrifuged again. ...19957790599
fatal sepsis due to a beta-lactamase-producing strain of fusobacterium nucleatum subspecies polymorphum.a patient with waldenström's macroglobulinemia was treated empirically with imipenem for sepsis related to oropharyngeal infection and responded within 24 hours. when blood cultures yielded streptococcus agalactiae, the regimen was changed to ampicillin and gentamicin. the patient's condition rapidly deteriorated, and she died 3 days later. after her death, a strain of fusobacterium nucleatum subspecies polymorphum producing beta-lactamase (pen-y; group 2a) was isolated from blood cultures. a li ...19957795076
microbial flora of sinus tracts and root canals of non-vital teeth.the occurrence of bacteria in 12 endodontically induced periodontal lesions associated with sinus tracts was examined. the microbial flora encountered in the sinus tract was compared with that of the root canal of the involved teeth which had not experienced any prior endodontic therapy. all microbiological samples taken from the sinus tract and from the root canal system contained bacteria. seventy-one strains were detected in the extraradicular lesions. of the anaerobic species, fusobacterium ...19957641609
bacteremia in conjunction with endodontic therapy.this study characterizes oral microorganisms believed to have spread from the root canal into the blood stream during and after endodontic therapy of teeth with asymptomatic apical periodontitis. microbiological samples were taken under aseptic conditions from the root canal of 26 single-rooted teeth in 26 patients. in the endodontic treatment of 13 of the patients (group 1), the first 3 reamers, sizes 15, 20 and 25, were used to a level 2 mm beyond the apical foramen. in the other 13 patients ( ...19957641631
isolation of porphyromonas gingivalis strain from tubal-ovarian abscess.an unusual case of involvement of porphyromonas gingivalis is described. two anaerobic isolates, identified as fusobacterium nucleatum and p. gingivalis, were recovered from the pus of a tubal-ovarian abscess in a 35-year-old woman. identification of the p. gingivalis isolate was confirmed by randomly amplified polymorphic dna fingerprinting.19957665673
[spondylodiscitis caused by fusobacterium nucleatum. apropos of a case].spondylodiscitis is rarely caused by anaerobic germs and occurs mainly in patients weakened by another infection. we report a case of fusobacterium nucleatum spondylodiscitis in a 63-year-old man. this is the third such report and the second in a non-immunodepressed patient. the clinical presentation and laboratory findings in this type of spondylodiscitis vary little from those produced by other germs. magnetic resonance imaging is the most useful diagnostic tool for spondylodiscitis. the germ ...19957667223
the influence of intracellular polyglucose and prior growth rate on the survival of fusobacterium nucleatum under starvation conditions.grown in a chemically defined medium containing glucose at a dilution rate of d = 0.065 h-1, fusobacterium nucleatum d212b-2 produced large amounts of intracellular polyglucose. aliquots of this culture were starved by anaerobic incubation at 37 degrees c and at various times, assayed for intracellular polyglucose content and viability. this protocol was repeated using cells grown under the same conditions in a chemically defined medium, a medium lacking carbohydrate and in which the organism pr ...19957675517
predominant microflora of severe, moderate and minimal periodontal lesions in young adults with rapidly progressive periodontitis.the purpose of this investigation was to study the microflora of severe, moderate and minimal periodontal lesions, in young adults with rapidly progressive periodontitis (rpp). subgingival plaque samples were taken from 142 periodontal lesions in 10 young adults aging 25 to 35 years. the examination of the subgingival microflora indicated that certain species, including porphyromonas gingivalis, bacteroides forsythus, fusobacterium nucleatum, actinobacillus actinomycetemcomitans, and campylobact ...19957722848
bacterial-stimulated cytokine production of peripheral mononuclear cells from patients of various periodontitis categories.periodontitis is a general term for disease categories, including juvenile periodontitis (jp), rapidly progressive periodontitis (rpp), and adult periodontitis (ap), which may or may not share a common etiology and pathogenesis. these disease categories are characterized by differences in progression of tissue destruction and differences in age group susceptibility, but not, to our knowledge, by differences in cytokine responses of inflammatory cells. the present study examined blood cell counts ...19957730965
[cerebral septic metastasis associated with hepatic abscess]. 19957734502
1h mrs of human brain abscesses in vivo and in vitro.five patients, each with a brain abscess, were examined by means of 1h mr spectroscopic imaging in vivo. the aspirated pus was analyzed in vitro by means of 1d and 2d cosy 1h mrs. in addition to resonance lines from compounds (lactate, alanine and lipids) often found in the spectra from intracranial tumors, resonance lines were detected from a number of markers of infectious involvement (acetate, succinate, and various amino acids). these results suggest that 1h mrs in vivo might contribute in e ...19958524016
competition for peptides and amino acids among periodontal bacteria.we recently studied the utilization of glutathione (l-gamma-glutamyl-l-cysteinylglycine), l-cysteinylglycine and l-cysteine by anaerobic bacteria. the rate of hydrogen sulfide formation from these compounds was determined and it was concluded that peptostreptococcus micros and fusobacterium nucleatum subsp. nucleatum had an active transport of small peptides. in the present study it is shown that methyl mercaptan formation from l-methionine and l-methionyl-containing peptides can also be used to ...19958544102
aerobic and anaerobic microbiology of superficial suppurative thrombophlebitis.to study the aerobic and anaerobic microbiologic characteristics of superficial suppurative thrombophlebitis.19968546586
host defensive functions in a family manifesting early-onset periodontitis.family case studies help us identify host risk factors in periodontal disease. in this study we examine a family consisting of a mother (40 years old, with rapidly progressive periodontitis), her elder daughter (14 years old, with localized juvenile periodontitis), and younger daughter (13 years old, with simple gingivitis). we examined 1) the peripheral neutrophil functions (chemotactic migration, phagocytosis, superoxide production); 2) lymphocyte functions (proliferative activity and cytokine ...19968708971
histological and microbiological aspects of ligature-induced periodontitis in beagle dogs.this study was designed to investigate, using 6 beagle dogs, the levels of selected putative pathogens in healthy sites, in gingivitis sites, and in sites with histologically confirmed attachment loss. levels of attachment loss increased with increasing periods of ligation and reached a maximum of 0.15 mm at 57 days. both histological attachment level and histological pocket depth were found to vary significantly with health/disease status (p < 0.0001). higher numbers of total colony-forming uni ...19968739161
response to periodontal therapy in patients with high or low levels of p. gingivalis, p. intermedia, p. nigrescens and b. forsythus.in a previous study, subjects receiving either adjunctive tetracycline or augmentin showed, on average, more attachment level gain 10 months post-therapy than subjects receiving either ibuprofen or a placebo, although some subjects in each treatment group showed loss of attachment post-therapy. since differences in treatment response might have been due to differences in the subgingival microbiota, the response to different therapies in subjects with different pre-therapy subgingival microbiotas ...19968739165
the effect of age on the development of gingivitis. clinical, microbiological and histological findings.in the present "experimental gingivitis" study, the response of the marginal gingiva to plaque formation was studied in one group of young subjects, 20-25 years of age, and in one group of older subjects, aged 65-80 years. during a 4-week period, all subjects received a series of professional tooth cleaning to establish healthy gingival conditions. a baseline examination (day 0) included assessments of plaque and gingivitis. sites that were examined (the experimental sites) included the mesio-pa ...19968739171
comparison of spiral gradient endpoint and agar dilution methods for susceptibility testing of anaerobic bacteria: a multilaboratory collaborative evaluation.a multilaboratory collaborative study was carried out to assess the utility of the spiral gradient endpoint (sge) method for the determination of the antimicrobial susceptibilities of anaerobes and to evaluate the equivalence of the mics obtained by the sge method with those obtained by the reference agar dilution method of the national committee for clinical laboratory standards. the standard deviation of the mic obtained by the sge method for the five participating laboratories was +/- 0.26 of ...19968748295
the effect of chlorhexidine on defined, mixed culture oral biofilms grown in a novel model system.in order to develop an improved method to evaluate antimicrobial agents for use in clinical dentistry, a constant-depth film fermenter (cdff) has been used to generate biofilms of fixed depth comprising nine species of bacteria commonly found in dental plaque in health and disease. these bacteria were grown together initially in a conventional chemostat which was used to inoculate the cdff over an 8 h period. medium was then supplied directly to the cdff and biofilms allowed to develop. the biof ...19968760321
effects of sublethal exposure to an antiseptic mouthrinse on representative plaque bacteria.although the mechanism responsible for the clinical antiplaque efficacy of oral antiseptics is generally considered to be primarily one of bactericidal activity, it has been suggested that oral antiseptics may have additional effects on bacteria exposed to sublethal levels. studies reported herein, investigated the effects of sublethal levels of an essential oil-containing antiseptic mouthrinse (listerine antiseptic, warner-lambert co., morris plains, nj) on selected activities of representative ...19968783049
rapid characterization of periodontal bacterial isolates by using fluorogenic substrate tests.eighty-nine species of subgingival bacteria, represented by 121 reference strains and 892 patient isolates, including gram-negative, gram-positive, aerobic, facultatively anaerobic, microaerophilic, and anaerobic species, were characterized with a panel of fluorogenic, 4-methylumbelliferyl-linked substrate tests. identifications of all patient isolates were confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of whole-cell proteins relative to reference strains. char ...19968789019
treatment of periodontal disease based on microbiological diagnosis. relation between microbiological and clinical parameters during 5 years.the purpose of this study was to assess the clinical effect of treatment aimed to suppress actinobacillus actinomycetemcomitans and porphyromonas gingivalis below detection level and prevotella intermedia below 5% of the cultivable periodontal pocket flora. sixteen patients and a total of 111 periodontal pockets with probing depth > or = 6 mm were included in the study. twelve patients and a total of 77 sites completed the 5-year study. the results demonstrated clinical improvement of probing de ...19968794965
microbiology of periapical abscesses and associated maxillary sinusitis.aspirate of pus from 5 periapical abscesses of the upper jaw and their corresponding maxillary sinusitis were studied for aerobic and anaerobic bacteria. polymicrobial flora were found in all instances, where the number of isolates varied from 2 to 5. anaerobes were recovered from all specimens. the predominant isolates were prevotella sp., porphyromonas sp., fusobacterium nucleatum, and peptostreptococcus sp. concordance in the microbiological findings between periapical abscess and the maxilla ...19968794972
prevalence of 6 putative periodontal pathogens in subgingival plaque samples from romanian adult periodontitis patients.the aim of the present study was to determine by standard cultivation procedures the detection frequencies of porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, actinobacillus actinomycetemcomitans, bacteroides forsythus, and capnocytophaga species as well as various enteric rods in subgingival plaque samples form romanian adult periodontitis patients. dna probe analysis (affirm dp microbial identification test) was also used, parallel to cultivation, to identify p. gingiv ...19968849850
growth inhibition of a human oral bacterium porphyromonas gingivalis by rat cysteine proteinase inhibitor cystatin s.agar diffusion analysis demonstrated that rat cystatin s, a cysteine proteinase inhibitor, inhibited the growth of all tested strains of a human oral, gram-negative anaerobic periodontopathogen porphyromonas gingivalis. its specific inhibitory activity against this tissue-invasive bacterium but not against other tested oral bacterial species emphasized the importance of specific cysteine proteinases for growth of p. gingivalis.19968862019
recurrent vertebral osteomyelitis and psoas abscess caused by streptococcus constellatus and fusobacterium nucleatum in a patient with atrial septal defect and an occult dental infection.we describe a case in which recurrent vertebral osteomyelitis and psoas abscess due to streptococcus constellatus and fusobacterium nucleatum developed in a patient with a previously unrecognized atrial septal defect with bidirectional shunts. disease recurrence was ascribed to the presence of dental disease, which served as the source of infection. to the best of our knowledge, this is the first case in which vertebral osteomyelitis and psoas abscess have been associated with atrial septal defe ...19968863368
effect of oxygen, inoculum composition and flow rate on development of mixed-culture oral biofilms.the effect of aeration on the development of a defined biofilm consortium of oral bacteria was investigated in a two-stage chemostat system. an inoculum comprising 10 species, including both facultatively anaerobic and obligately anaerobic bacteria, and species associated with oral health and disease, was inoculated into an anaerobic first-stage chemostat vessel. the effluent from this chemostat was linked to an aerated [200 ml co2/air (5:95, v/v) min-1] second-stage vessel, in which removable h ...19968868437
development of a steady-state oral microbial biofilm community using the constant-depth film fermenter.the complexity of biofilm communities like dental plaque suggests that laboratory model biofilm growth systems may help to understand their structure and function. this study describes the use of a constant-depth film fermenter (cdff) to investigate biofilm formation by a nine-membered community of oral bacteria. the community was grown to steady state in a chemostat incubated anaerobically. the chemostat output was fed into the cdff incubated aerobically. viable counts for each species from the ...19968868438
evaluation of the long-term efficacy and safety of locally-applied minocycline in adult periodontitis patients.the objectives of the present study were to establish in a long-term investigation the safety as well as the clinical and microbiological efficacy of scaling and rootplaning combined with local application of 2% minocycline hydrochloride-gel versus placebo-gel in patients with moderate to severe chronic adult periodontitis. this was an 18 months, randomized, double-blind, parallel, comparative study, in which 20 healthy patients with moderate to severe chronic periodontitis participated. at base ...19968877655
the relationship of gingival crevicular fluid short chain carboxylic acid concentration to gingival inflammation.short-chain carboxylic acids (scca; c < or = 5; e.g., lactic acid, propionic acid, butyric acid) are metabolic by-products of bacterial metabolism which accumulate in the gingival crevice, and exhibit significant biological activity, including the ability to alter gene expression. it has been hypothesized that among the activities of sccas are their ability to contribute to gingival inflammation. this concept complements the notion that specific periodontal pathogens are the causative agents of ...19968877660
acute tongue abscess: report of two cases.tongue abscesses are extremely rare. two cases caused by periodontal pathogens are presented.19968910119
strong cytotoxicity to human gingival fibroblasts by porphyromonas gingivalis atcc 33277.the aim of the present study was to analyze the cytotoxicity of some bacterial species associated with periodontal diseases. the specificity of cytotoxicity was estimated against cells of various origin and from different individuals. the reference bacteria were actinobacillus actinomycetemcomitans, porphyromonas gingivalis and fusobacterium nucleatum. these bacteria were cultured for 24 h in liquid media and the supernatants were used in cytotoxicity assays. the target cells used were human gin ...19968915950
serum rheumatoid factor induced by intraperitoneal administration of periodontopathic bacterial lipopolysaccharide in mice.serum rheumatoid factor (rf) level and peritoneal and splenic cd5+b (b-1) cells in mice were examined after intraperitoneal administration of purified lipopoly-saccharides (lps) from oral periodontopathic bacteria; porphyromonas gingivalis, actinobacillus actinomycetemcomitans, fusobacterium nucleatum and capnocytophaga ochracea. f. nucleatum and c. ochracea lps induced higher levels of serum igm- and igg-rf, while p. gingivalis lps showed the least induction. in addition, wet weights of spleen ...19968915954
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