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identification and sequencing of bacterial rdnas in culture-negative amniotic fluid from women in premature labor.our objective was to identify bacterial species present in culture-negative but 16s rdna-positive amniotic fluid samples from women in preterm labor. amniotic fluid from 69 women in preterm labor was cultured and examined for the proinflammatory cytokine interleukin-6 (il-6). polymerase chain reaction technology was used to detect highly conserved bacterial ribosomal dna sequences (16s rdnas). as previously reported, 16s rdnas were identified in 15 (94%) of 16 culture-positive amniotic fluid sam ...200415311367
implant therapy following liver transplantation: clinical and microbiological results after 10 years.the number of immunocompromised patients is increasing as a consequence of successful organ transplantation. placing dental implants in these patients has been questioned because of their increased risk for infections. the 10-year follow-up data of a 71-year-old liver transplant recipient with long-term immunosuppressive therapy is reported. six months after liver transplantation, two interforaminal implants were inserted in the edentulous mandible, and an overdenture using non-rigid telescopic ...200415295960
[purification and characterization of sanguicin--a bacteriocin produced by streptococcus sanguis].streptococcus sanguis plays an important role in maintaining the periodontal microecological balance. previous studies have demonstrated that sanguicin--a kind of bacteriocin produced by s. sanguis has a prominent function of inhibiting the growth of putative periodontopathic bacteria (ppb). the aim of this study was to purify sanguicin and investigate its characters.200415291127
active domains of salivary statherin on apatitic surfaces for binding to fusobacterium nucleatum cells.fusobacterium nucleatum can bind to saliva-coated tooth surfaces. however, the nature of the domains of salivary protein that interact with f. nucleatum remains unclear. the ability of individual proteins in human submandibular-sublingual saliva (hsmsl) to bind f. nucleatum cells was examined by dot blot assay; statherin displayed the strongest binding activity. statherin binding sites were determined based on binding of (125)i-labelled f. nucleatum to statherin-coated hydroxyapatite (shap) bead ...200415256578
development of a multispecies oral bacterial community in a saliva-conditioned flow cell.microbial communities within the human oral cavity are dynamic associations of more than 500 bacterial species that form biofilms on the soft and hard tissues of the mouth. understanding the development and spatial organization of oral biofilms has been facilitated by the use of in vitro models. we used a saliva-conditioned flow cell, with saliva as the sole nutritional source, as a model to examine the development of multispecies biofilm communities from an inoculum containing the coaggregation ...200415240317
portal vein thrombosis associated with fusobacterium nucleatum septicemia in a patient with ulcerative colitis. 200415232367
evaluation of the whole genome dna-dna hybridization technique to identify bacteria in histological sections of periradicular lesions.whole genome dna-dna hybridization has been used to identify bacteria in periradicular lesions partly because there is no amplification of the bacteria, therefore, minor contaminants are not detected. there are, however, potential pitfalls with this technique, including inability to distinguish dead bacteria, cross-reactions of species within a genus, and inability to detect species present in low numbers because of loss of dna during extraction and purification. alternatively, inadequate extrac ...200415220650
comparison of systemic flurithromycin therapy and clinical procedures in the treatment of periodontal diseases.the purpose of the present investigation was to evaluate, in 20 periodontal patients, the microbial and clinical effects of flurithromycin therapy plus scaling and root planning (srp) in comparison with srp alone. clinical assessments of plaque, bleeding on probing and pocket depth were made prior to srp alone and srp plus flurithromycin therapy (375 mg twice daily for 5 days) and after both treatments. subgingival plaque samples (n. 180) were taken prior to and after both treatments and analyze ...200415216949
subgingival microbiota of chilean patients with chronic periodontitis.an association between race/ethnicity and the composition of the subgingival microbiota has been found in chronic periodontitis. a study was undertaken to determine the characteristics of the subgingival microbiota of chronic periodontitis in chileans residing in santiago.200415212354
effect of supragingival plaque control on subgingival microflora and gingivo-periodontal tissues.the aim of this study was to carry out a short term evaluation of the effect of supragingival plaque control on the subgingival microflora and gingivoperiodontal tissues in patients with moderate and severe periodontitis. a total of 92 sites from 23 patients were studied; patients ranged between 35 and 60 years of age (x 46.4 +/- 1.71). plaque index (pi), gingival index (gi), bleeding on probing (bp), probing depth (pd), and attachment level (al) were measured. subgingival microflora of the same ...200015211927
modulation of the antibody response by porphyromonas gingivalis and fusobacterium nucleatum in a mouse model.successive immunization of mice with fusobacterium nucleatum and porphyromonas gingivalis has been shown to modulate the specific serum igg responses to these organisms. the aim of this study was to investigate these antibody responses further by examining the igg subclasses induced as well as the opsonizing properties of the specific antibodies. serum samples from balb/c mice immunized with f. nucleatum (gp1-f), p. gingivalis (gp2-p), p. gingivalis followed by f. nucleatum (gp3-pf) f. nucleatum ...200415209995
the effects of chlorhexidine gluconate (0.12%) on the antimicrobial properties of tooth-colored proroot mineral trioxide aggregate.the purpose of this in vitro study was to determine whether the substitution of 0.12% chlorhexidine gluconate for sterile water as a mixing agent would enhance the antimicrobial activity of tooth-colored proroot mineral trioxide aggregate (mta) against actinomyces odontolyticus (atcc17982), fusobacterium nucleatum (atcc2586), streptococcus sanguis (atcc10556), enterococcus faecalis (er3/2s), escherichia coli (sm10lambdapir), staphylococcus aureus (atcc6538), pseudomonas aeruginosa (ume), and can ...200415167473
perforation repair comparing two types of mineral trioxide aggregate.the purpose of this study was to evaluate the ability of two types of mineral trioxide aggregate (mta) to seal furcal perforations in extracted human molars using an anaerobic bacterial leakage model. forty, human, maxillary and mandibular molars were randomly divided into two experimental groups of 18, with two teeth used as positive controls and two teeth without perforations used as negative controls. experimental group 1 was repaired with gray-colored mta and group 2 with off-white-colored m ...200415167471
apical periodontitis development and bacterial response to endodontic treatment. experimental root canal infections in monkeys with selected bacterial strains.in six monkeys, 160 root canals were inoculated with a combination of four bacterial strains belonging to species streptococcus milleri, peptostreptococcus anaerobius, prevotella oralis, and fusobacterium nucleatum. in two other monkeys, 24 root canals were inoculated with a five-strain combination consisting of these strains and a strain of enterococcus faecalis. all strains were previously isolated from an infected monkey root canal. after 8-12 months, survival of the strains was recorded bact ...200415154917
clonal persistence of oral fusobacterium nucleatum in infancy.once established, early-colonizing bacterial species tend to persist in the mouth. to obtain detailed information on the population dynamics of early-colonizing oral anaerobes, we examined the clonal diversity and persistence of clones among oral fusobacterium nucleatum populations during the first 2 yrs of life. consecutive salivary samples from 12 infants, collected at 2, 6, 12, 18, and 24 mos of age, yielded a total of 546 f. nucleatum isolates for clonal typing with arbitrarily primed pcr (a ...200415153460
another good reason to floss. 200415141231
surveillance of susceptibility patterns in 1297 european and us anaerobic and capnophilic isolates to co-amoxiclav and five other antimicrobial agents.in vitro susceptibility data were collected for co-amoxiclav and other antimicrobial agents against 1297 recent anaerobe isolates collected in europe and the usa. the co-amoxiclav (amoxicillin/clavulanic acid) mic(50/90)s (amoxicillin/clavulanic acid concentration in a ratio of 2:1, expressed in terms of amoxicillin concentration in mg/l) were 0.5/4 for bacteroides fragilis, </=0.125/1 for prevotella species, </=0.125/0.25 for fusobacterium nucleatum, 0.5/1 for eikenella corrodens, 0.25/8 for pe ...200415128729
the role of lipopolysaccharide in infectious bone resorption of periapical lesion.the role of lipopolysaccharide (lps) in periapical lesion-induced bone resorption was investigated. polymyxin b (pmb), a specific inhibitor of lps, was evaluated to treat the apical lesion.200415128058
microbiologic diagnostics at titanium implants.the microbiota found at periimplant lesions have been shown to contain putative periodontal pathogens as well as opportunistic species such as staphylococcus spp, enterics, and candida spp. therefore, a microbiologic diagnosis may be of value as guidance before treatment of such lesions.200315127993
selection for high-level resistance by chronic triclosan exposure is not universal.to investigate the effect of triclosan exposure on the antimicrobial susceptibilities of numerically important dental bacteria.200415117935
induction of calprotectin release by porphyromonas gingivalis lipopolysaccharide in human neutrophils.calprotectin, a major cytosolic protein of leukocytes, is detected in neutrophils, monocytes/macrophages, and epithelial cells. this protein is known to be a marker for several inflammatory diseases and is detected in inflammatory gingival tissue with periodontal disease. recently, we found that the calprotectin level in gingival crevicular fluid from periodontitis patients was significantly higher than that of healthy subjects. however, the regulation of calprotectin in periodontal disease is u ...200415107070
identification of a fusobacterium nucleatum 65 kda serine protease.a 65 kda protease was partially purified from extracellular vesicles of fusobacterium nucleatum cultures by preparative sds-page followed by electroelution. the ph optimum of the protease is 7.5-8.0 and its activity could be inhibited by serine protease inhibitors. the protease was found to degrade the extracellular matrix proteins fibrinogen and fibronectin as well as collagen i and collagen iv which were degraded at 37 degrees c but not at 28 degrees c, indicating the presence of a gelatinase ...200415107066
risk factors for periodontitis in hiv patients.the purpose of this study was to identify risk factors for periodontitis associated with human immunodeficiency virus (hiv) infection.200415102043
blindness from septic thrombophlebitis of the orbit and cavernous sinus caused by fusobacterium nucleatum. 200415078687
[dynamic observation of periodontal pathogens and cariogenic bacteria in modified chemostat using confocal laser scanning microscopy].to study dynamic relation between periodontal pathogens and cariogenic bacteria under analogous oral environment.200415061891
geographical differences in bacteria detected in endodontic infections using polymerase chain reaction.the polymerase chain reaction (pcr) is an innovative nucleic acid-based assay that has the highest sensitivity of any microbiological technique for the detection of bacteria. the purpose of this study was to use pcr to detect the presence of specific species of bacteria in samples collected from two geographical locations. microbial samples from abscesses of endodontic origin were collected from patients in portland, oregon, and rio de janeiro, brazil. pcrs with species-specific oligonucleotide ...200415055430
fusobacterium nucleatum induces premature and term stillbirths in pregnant mice: implication of oral bacteria in preterm birth.fusobacterium nucleatum is a gram-negative anaerobe ubiquitous to the oral cavity. it is associated with periodontal disease. it is also associated with preterm birth and has been isolated from the amniotic fluid, placenta, and chorioamnionic membranes of women delivering prematurely. periodontal disease is a newly recognized risk factor for preterm birth. this study examined the possible mechanism underlying the link between these two diseases. f. nucleatum strains isolated from amniotic fluids ...200415039352
human beta-defensins 2 and 3 demonstrate strain-selective activity against oral microorganisms.human beta-defensins 2 and 3 (hbd-2 and hbd-3) are inducible peptides present at sites of infection in the oral cavity. a few studies have reported broad-spectrum antimicrobial activity for both peptides. however, no comprehensive study has thoroughly investigated their potential against oral pathogens. the purpose of this study was to test the effectiveness of hbd-2 and hbd-3 against a collection of oral organisms (actinobacillus actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gin ...200415004048
relationship between tongue coating and secretory-immunoglobulin a level in saliva obtained from patients complaining of oral malodor.the aim of this study was to confirm the relationships between oral malodor and periodontal condition, oral malodor and tongue coating, and to investigate the secretory-immunoglobulin a (s-iga) level in saliva in relation to the accumulation of tongue coating.200315002886
glyceraldehyde-3-phosphate dehydrogenase of streptococcus oralis functions as a coadhesin for porphyromonas gingivalis major fimbriae.cohesive interactions between porphyromonas gingivalis and plaque-forming bacteria, such as streptococcus oralis, are considered to play an important role in the colonization of p. gingivalis in periodontal sites. although p. gingivalis fimbriae have been reported to mediate coaggregation with s. oralis, the s. oralis molecule involved has not been identified. we identified the coadhesin of s. oralis atcc 9811 and purified it by affinity column chromatography. we found that the molecular mass of ...200414977937
association of t cd4 lymphocyte levels and subgingival microbiota of chronic periodontitis in hiv-infected brazilians under haart.the aim of this study was to determine the subgingival microbiota of hiv-infected patients with chronic periodontitis and different t cd4 lymphocyte levels under haart.200414970778
[acute abscess of the cerebrum indicative of bronchiectasis, caused by fusiformis fusiformis associated with corynebacterium parvum; cure by combined surgical and anti-infectious treatment]. 195214935821
microbiological examination of infected dental root canals.the aim of this study was to investigate the root canal microbiota of primary and secondary root-infected canals and the association of constituent species with specific endodontic signs and symptoms.200414871344
gingival crevice microbiota from chinese patients with gingivitis or necrotizing ulcerative gingivitis.the objective of this study was to quantitatively compare the bacterial population structure in plaque from the gingival margin of two groups of 21 chinese patients with gingivitis or necrotizing ulcerative gingivitis (nug). subjects were recruited in four dental clinics in eastern china. samples were quantitatively assessed by immunofluorescence and fluorescent in situ hybridization for taxa known to be associated with periodontal diseases. the analyses showed that the fusiform taxa (fusobacter ...200414871191
relationship between periodontal pocket sulfide levels and subgingival species.many species implicated in the pathogenesis of periodontal disease produce volatile sulfur compounds (vsc). this investigation examined the relationship between levels of sulfide and subgingival bacterial species in the same periodontal pockets.200314761124
effect of porphyromonas gingivalis vesicles on coaggregation of staphylococcus aureus to oral microorganisms.vesicles from the outer membrane of porphyromonas gingivalis have the ability to aggregate a wide range of streptococcus spp., fusobacterium nucleatum, actinomyces naeslundii, and actinomyces viscosus. we found that in the presence of p. gingivalis vesicles, staphylococcus aureus coaggregated with streptococcus spp., and the mycelium-type candida albicans, but not the yeast type. autoaggregation of s. aureus in the presence of p. gingivalis vesicles is inhibited by l-arginine, l-lysine, and l-cy ...200314756532
clonal similarity of salivary and nasopharyngeal fusobacterium nucleatum in infants with acute otitis media experience.the environment of an infant's nasopharynx during acute otitis media (aom) favours the growth of anaerobic bacteria, which can be recovered frequently during infection, but hardly at all if the infant is healthy. the aim of this investigation was to identify the potential source and inoculation route of anaerobes that were present in the nasopharynx. eleven fusobacterium nucleatum isolates that were collected through the nasal cavity from the nasopharynx of eight infants with a history of aom, a ...200414729939
multiplex fish analysis of a six-species bacterial biofilm.established procedures use different and seemingly incompatible experimental protocols for fluorescent in situ hybridization (fish) with gram-negative and gram-positive bacteria. the aim of this study was to develop a procedure, based on fish and confocal laser scanning microscopy (clsm), for the analysis of the spatial organization of in vitro biofilms containing both gram-negative and gram-positive oral bacteria. biofilms composed of the six oral species actinomyces naeslundii, candida albican ...200414706749
innate immune response of oral and foreskin keratinocytes: utilization of different signaling pathways by various bacterial species.the innate immune response is critical for the epithelial antimicrobial barrier. the human beta-defensins are small, cationic antimicrobial peptides that are made by epithelial cells and that play a role in mucosal and skin defenses. human beta-defensin 1 (hbd-1) is expressed constitutively in epithelial tissues, whereas hbd-2 and hbd-3 are expressed in response to bacterial stimuli or inflammation. previous studies showed that hbd-2 was induced by fusobacterium nucleatum cell wall extract witho ...200414688115
detection of subgingival calculus and dentine caries by laser fluorescence.detection of subgingival calculus and dentine caries in the bottom of deep periodontal pockets is often difficult without visual observation. we thus examined the possibility of its detection using autofluorescence induced by laser irritation.200414687229
a comparison of the antibacterial efficacies of essential oils against oral pathogens.cariogenic bacteria and periodontopathic bacteria are present in dental plaque as biofilms. in this study, we investigated the antibacterial effects of essential oils on the following oral bacteria: porphyromonas gingivalis, actinobacillus actinomycetemcomitans, fusobacterium nucleatum, streptococcus mutans, and streptococcus sobrinus. we tested manuka oil, tea tree oil, eucalyptus oil, lavandula oil, and romarinus oil and determined their minimum inhibitory concentration and minimum bactericida ...200414678476
culture-independent identification of pathogenic bacteria and polymicrobial infections in the genitourinary tract of renal transplant recipients.renal transplant recipients are predisposed to urinary tract infections caused by both common uropathogens and opportunistic bacteria resulting frequently in significant polymicrobial infections. in this study, a culture-independent 16s rrna-based approach was established to identify unusual, fastidious, or anaerobic bacteria and to investigate bacterial diversity in urinary tract specimens. similarly sized amplicons encompassing the v6 to v8 region of the 16s rrna were analyzed with denaturing ...200314662931
antagonistic interactions among fusobacterium nucleatum and prevotella intermedia with oral lactobacilli.microbiota residing in oral ecosystems show great complexity because of the mutual interdependence of certain microorganisms that might influence their presence or absence at a given site. the aim of this work was to evaluate possible antagonistic interactions among fusobacterium nucleatum and prevotella intermedia, isolated from periodontal pockets, and lactobacillus casei, l. salivarius, l. rhamnosus and l. plantarum isolated from saliva. different tests were carried out using each strain as a ...200314643404
the influence of organic load on the antimicrobial activity of different concentrations of naocl and chlorhexidine in vitro.to evaluate bacterial growth after contact with sodium hypochlorite (naocl; 1 and 5%) and chlorhexidine (chx; 0.12, 0.5 and 1%) in vitro with or without the addition of organic material (bovine serum albumin (bsa) 0.5%).200314641423
expression of mhc class ii, cd70, cd80, cd86 and pro-inflammatory cytokines is differentially regulated in oral epithelial cells following bacterial challenge.oral epithelium may play a regulatory role in local immune responses when interacting with bacteria. the present study was undertaken to investigate the effects of selected bacterial pathogens found in periodontal and endodontic infections on oral epithelial cells. expression of cell surface molecules (major histocompatibility complex (mhc) class ii, cd54, cd70, cd80 and cd86) and secretion of inflammatory cytokines (interleukin (il)-1beta, il-6, and tumor necrosis factor (tnf)-alpha) in respons ...200314622340
fusobacterium nucleatum: a rare cause of bacteremia in neutropenic patients with leukemia and lymphoma.although anaerobic bacteremias are uncommon in oncohematologic patients, nevertheless they have been considered an emergent problem in the last few years. fusobacterium nucleatum is an anaerobic gram-negative bacillus commonly present in the oral cavity and in the respiratory and genito-urinary tracts. over a 10-year period 18 episodes of f. nucleatum bacteremia in patients with hematological malignances (15 leukemias and 3 lymphomas) have been observed in our department of hematology. predispos ...200314616727
clinical presentation and bacteriologic analysis of infected human bites in patients presenting to emergency departments.previous studies of infected human bites have been limited by small numbers of patients and suboptimal microbiologic methodology. we conducted a multicenter prospective study of 50 patients with infected human bites. seventy percent of the patients and assailants were young adult men. fifty-six percent of injuries were clenched-fist injuries and 44% were occlusional bites. most injuries were to the hands. fifty-four percent of patients were hospitalized. the median number of isolates per wound c ...200314614671
intracellular calcium in signaling human beta-defensin-2 expression in oral epithelial cells.expression of human beta-defensins is correlated with differentiation in the oral epithelium, consistent with their function as part of the epithelial antimicrobial barrier. because calcium is a known regulator of epithelial differentiation, we tested the hypothesis that calcium concentration mediates beta-defensin expression. gingival epithelial cells were cultured in medium containing low calcium concentration (0.03 mm), then either changed to high extracellular calcium concentrations or stimu ...200314578498
[ribotyping fusobacterium nucleatum isolates from healthy and diseased periodontium].fusobacterium nucleatum is one of the most frequently cultured bacteria from subgingival plaque. it can be isolated from active periodontal lesions as well as from healthy sites. currently, f. nucleatum has been divided into five subspecies. an early hypothesis suggested an association between certain subspecies and periodontal disease or health. on the other hand, a broad heterogeneity between f. nucleatum isolates has been suggested, so that the subspeciation scheme and therefore the proposed ...200314558378
intracellular production and extracellular release of oxygen radicals by pmns and oxidative stress on pmns during phagocytosis of periodontopathic bacteria.in this study we investigated intracellular and extracellular oxygen radical production by polymorphonuclear leukocytes (pmns) during the phagocytosis of periodontopathic bacteria. in in vitro assays, bacteria of the species porphyromonas gingivalis, actinobacillus actinomycetemcomitans, and fusobacterium nucleatum were phagocytosed at 37 degrees c for 4 h by purified peripheral human pmns from healthy subjects (n = 6). superoxide production during phagocytosis was determined by flow cytometry a ...200314505184
establishment of human indigenous bacteria in germ-free mice.gibbons, r. j. (forsyth dental center and harvard school of dental medicine, boston, mass.), s. s. socransky, and b. kapsimalis. establishment of human indigenous bacteria in germ-free mice. j. bacteriol. 88:1316-1323. 1964.-thirteen strains of bacteria indigenous to the gingival crevice area of man were tested for their ability to establish as monocontaminants in germ-free mice. three facultative organisms, streptococcus mitis, staphylococcus albus, and a "diphtheroid," established, as well as ...196414234787
antigenic characteristics of leptotrichia buccalis (fusobacterium fusiforme).gustafson, robert l. (university of illinois college of medicine, chicago) and arthur v. kroeger. antigenic characteristics of leptotrichia buccalis (fusobacterium fusiforme). j. bacteriol. 84:1313-1320. 1962.-thirty-nine isolates of an oral filamentous organism, usually termed leptotrichia buccalis trevisan (fusobacterium fusiforme), were isolated from materia alba or saliva. the cultural, morphological, and biochemical characteristics were similar to those reported by other investigators. aggl ...196213951402
[studies on fusobacteria. iii. in vitro test on natural resistance to fusobacterium plauti-vincenti]. 195713434351
[studies on fusiformis nucleatus (knorr) and its pathogenicity for humans and animals]. 195613395022
[pseudo-angiocholitic necrosing icterogenic hepatitis due to fusiformis nucleatus]. 195613359994
immune response to fusobacterium nucleatum and prevotella intermedia in the sputum of patients with acute exacerbation of chronic bronchitis.to assess the role of anaerobic bacteria in acute exacerbation of chronic bronchitis (aecb).200312970005
antimicrobial activity of different concentrations of naocl and chlorhexidine using a contact test.the purpose of this study was to analyze the in vitro antimicrobial activity of sodium hypochlorite (1% and 5%) and chlorhexidine (0.12%, 0.5% and 1%). bacterial samples (atcc) of staphylococcus aureus, enterococcus faecalis, escherichia coli, porphyromonas gingivalis and fusobacterium nucleatum were submitted to a contact test. solutions were evaluated at different time intervals: immediately, 5 min, 15 min, and 30 min after contact and repeated 10 times. the results of the contact test showed ...200312964652
the antimicrobial efficacy of 'mgp' gutta-percha in vitro.to determine whether 'mgp' gutta-percha (westport, ct, usa), a commercially available gutta-percha containing iodoform, inhibits the growth of potential endodontic pathogens.200312950576
multiple brain abscesses caused by fusobacterium nucleatum treated conservatively.multiple brain abscesses are serious neurological problems with high mortality and disabling morbidity. the frequency is rising as a result of aids and the increasing number of immunocompromised patients.200312945954
coaggregation of streptococcus salivarius with periodontopathogens: evidence for involvement of fimbriae in the interaction with prevotella intermedia.streptococcus salivarius is divided into two serological subgroups that carry either fibrils or fimbriae. although fimbriae have been observed on up to 50% of s. salivarius strains in the human oral cavity, no function has yet been assigned to them. to determine whether s. salivarius fimbriae have a role in adhesion, we examined the ability of s. salivarius to coaggregate with selected microorganisms involved in periodontal diseases. our results show that s. salivarius coaggregated with fusobact ...200312930529
effects of a chlorhexidine gluconate-containing mouthwash on the vitality and antimicrobial susceptibility of in vitro oral bacterial ecosystems.oral bacterial microcosms, established using saliva inocula from three individuals, were maintained under a feast-famine regime within constant-depth film fermenters. steady-state communities were exposed four times daily, postfeeding, to a chlorhexidine (chx) gluconate-containing mouthwash (chxm) diluted to 0.06% (wt/vol) antimicrobial content. the microcosms were characterized by heterotrophic plate counts and pcr-denaturing gradient gel electrophoresis (dgge). chxm caused significant decrease ...200312902270
antimicrobial constituents from goldenseal (the rhizomes of hydrastis canadensis) against selected oral pathogens.two new c-methyl flavonoids, 6,8-di- c-methylluteolin 7-methyl ether (1) and 6- c-methylluteolin 7-methyl ether (2), were isolated from a commercially available sample of the roots of hydrastis canadensis, along with seven known compounds, berberine (3), beta-hydrastine (4), canadine (5), canadaline (6), isocorypalmine (7), canadinic acid (8), and beta-sitosterol 3- o-beta- d-glucoside (9). the structures of the new compounds 1 and 2 were elucidated on the basis of their spectral data including ...200312898417
antibiotic resistance of pathogenic bacteria from odontogenic infections in taiwan.the purpose of this study was to evaluate the susceptibility of facultative and anaerobic odontogenic infectious flora to various antibiotics. we assessed 178 bacterial strains isolated from 74 patients with odontogenic infections. the e-test was used to determine susceptibility. the microbial flora was predominantly facultative gram-positive organisms and anaerobic gram-negative bacilli. the results of antimicrobial susceptibility test showed that ampicillin resistance was found with a very hig ...200312886961
positive and negative bacterial associations involving dialister pneumosintes in primary endodontic infections.dialister pneumosintes is an anaerobic gram-negative rod that has been recently implicated as a candidate endodontic pathogen. in this study, samples taken from abscessed teeth and infected root canals associated with asymptomatic or symptomatic periradicular lesions were examined for the occurrence of bacterial associations involving d. pneumosintes. dna was extracted from the samples, and the presence of d. pneumosintes and 16 other bacterial species was determined by means of species-specific ...200312877258
characteristics of brain abscess with isolation of anaerobic bacteria.in view of its localization, brain abscess (ba) usually requires medical and surgical care. a broad spectrum of bacteria is involved. recent reports stress the increasing frequency of anaerobes, but their impact has not been well evaluated. a retrospective review was conducted of all episodes of documented ba admitted in a tertiary-care hospital over a 10 y period. ba due to anaerobic bacteria (group a) were compared with other cases (group b) to determine the frequency and eventual characterist ...200312875518
unusual presentation of lemierre's syndrome due to fusobacterium nucleatum.we report a case of lemierre's syndrome due to fusobacterium nucleatum in a previously healthy 19-year-old male. this is the first case report of lemierre's syndrome due to thrombophlebitis of the external jugular vein. the patient had a rapid clinical response to anticoagulation and antibiotics, as supported by anecdotal evidence.200312843117
effect of smoking and periodontal treatment on the subgingival microflora.the effect of smoking on the prevalence of periodontal pathogens after periodontal treatment is still not clear. some studies found no effect of the smoking status on the prevalence of periodontal pathogens after therapy, whereas others did. the aim of this retrospective study was to investigate the influence of smoking on the treatment of periodontitis and the composition of the subgingival microflora.200312834497
lethal photosensitization of periodontal pathogens by a red-filtered xenon lamp in vitro.the ability of helium-neon (he-ne) laser irradiation of a photosensitizer to induce localized phototoxic effects that kill periodontal pathogens is well documented and is termed photodynamic therapy (pdt).200312828662
changes in growth and polyglucose synthesis in response to fructose metabolism by fusobacterium nucleatum grown in continuous culture.fusobacterium nucleatum, grown in a chemically defined medium at micro(rel) = 0.5, produced greater cell yields and undetectable levels of intracellular polyglucose (ip) when fructose was substituted for glucose. the utilisation and metabolism of fructose by growing cells was studied and the effect of the energy-yielding amino acids, glutamate, serine, histidine and lysine on cell yield, ip synthesis and acidic end-products was investigated. when f. nucleatum was grown on elevated amino acid lev ...200312823803
bacteria-binding plasma proteins in pellicles formed on hydroxyapatite in vitro and on teeth in vivo.previous studies on dental pellicle formation and bacterial adherence have focussed on saliva and its components. the tooth surface is, however, also exposed to the plasma-derived crevicular fluid. in the present study, (i). plasma proteins in in vitro and in vivo pellicles were examined using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page), immunoblotting and image analysis and (ii). the adherence of periodontopathogenic bacteria to experimental plasma and saliva pellicles ...200312823794
antibacterial activity of medicinal plant extracts against periodontopathic bacteria.this study was performed to evaluate the antibacterial activity of althaea officinalis l. roots, arnica montana l. flowers, calendula officinalis l. flowers, hamamelis virginiana l. leaves, illicium verum hook. fruits and melissa officinalis l. leaves, against anaerobic and facultative aerobic periodontal bacteria: porphyromonas gingivalis, prevotella spp., fusobacterium nucleatum, capnocytophaga gingivalis, veilonella parvula, eikenella corrodens, peptostreptococcus micros and actinomyces odont ...200312820224
genome analysis of f. nucleatum sub spp vincentii and its comparison with the genome of f. nucleatum atcc 25586.we present the draft genome sequence and its analysis for fusobacterium nucleatum sub spp. vincentii (fnv), and compare that genome with f. nucleatum atcc 25586 (fn). a total of 441 fnv open reading frames (orfs) with no orthologs in fn have been identified. of these, 118 orfs have no known function and are unique to fnv, whereas 323 orfs have functional orthologs in other organisms. in addition to the excretion of butyrate, h2s and ammonia-like fn, fnv has the additional capability to excrete l ...200312799352
genomics of oral bacteria.advances in bacterial genetics came with the discovery of the genetic code, followed by the development of recombinant dna technologies. now the field is undergoing a new revolution because of investigators' ability to sequence and assemble complete bacterial genomes. over 200 genome projects have been completed or are in progress, and the oral microbiology research community has benefited through projects for oral bacteria and their non-oral-pathogen relatives. this review describes features of ...200312799321
subgingival microbiota levels and their associations with periodontal status at the sampled sites in an adult sudanese population using miswak or toothbrush regularly.little information is available on the effect of miswak use on gingival microbiota. we assessed levels of 28 oral bacteria in subgingival plaque of adult sudanese miswak (n = 38) and toothbrush users (n = 36) age range 20-53 years (mean 34.6 years) to study associations between these bacteria, oral hygiene method, and periodontal status at the sampled sites. a pooled subgingival plaque sample from 6 probing sites of 1 selected tooth in each jaw was obtained from each subject. whole genomic dna p ...200312790510
evaluation of antibacterial activity of mangifera indica on anaerobic dental microglora based on in vivo studies.the present study was carried out to compare the subgingival plaque microflora in mango leaf users. fifty subjects of both sexes, 25 of them used tooth brush and 25 used mango leaf as their home care hygiene device were included in the study. the microbiological evaluation for specific bacterial counts of actinobacillus actinomycetemcomitans, prevotella intermedia, porphyromonas gingivalis, fusobacterium nucleatum and peptostreptococcus micros were carried out for all subject. specific microbial ...200212785172
additive antimicrobial activity of calcium hydroxide and chlorhexidine on common endodontic bacterial pathogens.it is an established procedure to use calcium hydroxide [ca(oh)2] as a therapeutic component to achieve sterilization of infected root canals. unfortunately, some bacterial species are relatively resistant to ca(oh)2 and are therefore associated with treatment failures. the objective of this study was to identify combinations of substances that improve antimicrobial activity. ca(oh)2 was used as a suspension or as a component of gutta-percha points. efficacy of killing was compared for (a) ca(oh ...200312775007
[early diagnosis of bacterial brain abscesses: interest of diffusion-weighted mri].three cases of bacterial brain abscesses, in immunocompetent patients, are reported. in all these cases, the diffusion-weighted magnetic resonance (mri) with apparent diffusion coefficient (adc) map has permitted an early diagnosis and a rapid treatment. this emergency mri showed in the three cases a low signal on ti-weighted images, a high signal on t2-weighted and echo-planar images, and a decrease of adc (0.36- 0.49 x 10(-3) mm2/s). so, this new mri technique provides an available and rapid e ...200312773871
actinobacillus actinomycetemcomitans serotype b lipopolysaccharide mediates coaggregation with fusobacterium nucleatum.purified actinobacillus actinomycetemcomitans serotype b lipopolysaccharide (lps) was found to be able to bind fusobacterium nucleatum cells and to inhibit binding of f. nucleatum to a. actinomycetemcomitans serotype b. sugar binding studies showed that the requirements for binding of a. actinomycetemcomitans serotype b lps to the f. nucleatum lectin are the presence of a metal divalent ion, an axial free hydroxyl group at position 4, and free equatorial hydroxyl groups at positions 3 and 6 of d ...200312761156
pcr method is essential for detecting mycobacterium tuberculosis in oral cavity samples.tuberculosis is a re-emerging infectious disease, and infection by mycobacterium tuberculosis has been increasing in immunocompromised hosts, including elderly persons. m. tuberculosis-infected persons may receive dental treatment. to evaluate the risk of m. tuberculosis infection in dental clinics, we examined the detection rates of m. tuberculosis in sample of mixed saliva, dental plaque, extracted teeth, caries lesions, and denture plaque by nested polymerase chain reaction (pcr). the detecti ...200312753466
plasmid profile in oral fusobacterium nucleatum from humans and cebus apella monkeys.fusobacterium nucleatum is a strict anaerobe and is indigenous of the human oral cavity. this organism is commonly recovered from different monomicrobial and mixed infections in humans and animals. in this study, the plasmid profile, the plasmid stability and the penicillin-resistance association in oral f. nucleatum isolated from periodontal patients, healthy subjects and cebus apella monkeys were evaluated. forty-five f. nucleatum strains from patients, 38 from healthy subjects and seven from ...200312751315
bacterial penetration through canals of endodontically treated teeth in the presence or absence of the smear layer.the goal of this study is to determine if the smear layer affects the passage of bacteria through or around obturating material as evidenced by penetration of bacteria through and out the canal. specifically, this study focused on determining the effect of the smear layer on the magnitude of bacterial penetration through the apical foramen.200312735922
[extrinsic ureteral obstruction secondary to inflammatory gynecologic pathology].to present two cases of extrinsic ureteral obstruction secondary to gynaecological inflammatory diseases, and to perform a bibliographic review.200312731448
the effects of a new mouthrinse containing chlorhexidine, cetylpyridinium chloride and zinc lactate on the microflora of oral halitosis patients: a dual-centre, double-blind placebo-controlled study.this study evaluated the microbial effects of a newly formulated mouthwash (halita) on oral halitosis patients.200312716335
can toothpaste or a toothbrush with antibacterial tufts prevent toothbrush contamination?periopathogens and cariogenic species survive more than 48 hours on toothbrushes even under dry storage. this study examined a decontamination of toothbrushes by means of the bactericidal effect of different toothpastes or a special coating of the tufts.200312710750
ovine periodontitis as a potential model for periodontal studies. cross-sectional analysis of clinical, microbiological, and serum immunological parameters.: to investigate infection and host immunity patterns in sheep with naturally occurring "broken-mouth" periodontitis.200312702113
ultrastructural analysis of an infected collagen-coated vascular graft.the incidence of infection following arterial reconstruction using synthetic graft materials varies from less than 1 to 5%. one of three mechanisms is thought to be responsible: 1. intraoperative contamination, 2. extension from adjacent infected or colonized tissue, or 3. hematogenous or lymphogenous seeding. we present ultrastructural data of a patient with a polymicrobial graft infection due to a prostheto-enteric fistula 16 years after reconstruction of an aortobifemoral graft. the polymer s ...200312677763
palmitoleic acid isomer (c16:1delta6) in human skin sebum is effective against gram-positive bacteria.the percent lipid composition of pooled human sebum analyzed by thin-layer chromatography was: ceramides (13%), fatty acid (47%), cholesterol (7%), cholesterol esters (2%), squalene (11%), triglycerides (3%), and wax esters (17%). total sebum lipids (2- 4 mg/ml), sonicated into bacterial culture medium, caused 4- to 5-fold log reduction in growth of gram-positive bacteria, staphylococcus aureus, streptococcus salivarius and the anaerobe fusobacterium nucleatum, but was ineffective against most g ...200312677098
antimicrobial properties of self-etching primer-bonding systems.self-etching primers are now considered the new generation of dentin bonding systems that modify and incorporate the bacteria-containing smear layer into their bonding mechanism. the antibacterial effects of the self-etching primers clearfil se bond, mac bond, imperva fl bond, one-up bondf and prompt l-pop were evaluated using the bacteria streptococcus mutans atcc25175, peptostreptococcus anaerobius, peptostreptococcus prevotii, peptostreptococcus asaccharolyticus, lactobacillus acidophilus, la ...200312670069
an immunohistological study of the localization of bacteria invading root pulpal walls of teeth with periapical lesions.we immunohistologically examined the prevalence and localization of bacteria invading dentinal tubules of the roots of teeth with infected canals. forty extracted teeth with apical lesions were selected and divided into two groups: a group of untreated teeth and a group of canal-enlarged teeth. the bacteria in the specimens were detected by brown-brenn stain and the labeled-streptavidin-biotin method with specific antisera for 16-bacteria. seventy percent of the examined teeth showed bacteria in ...200312669880
serum phenytoin concentration and igg antibody titre to periodontal bacteria in patients with phenytoin-induced gingival overgrowth.epileptic patients taking phenytoin with gingival-overgrowth and those without gingival-overgrowth were compared for daily drug dose, plasma total phenytoin concentration, plasma free-phenytoin concentration and serum igg antibody titre against 13 periodontal bacteria. significantly higher daily drug dose was noted in patients with gingival overgrowth (p < 0.05) when compared with those without overgrowth. in addition, both total and free forms of plasma phenytoin concentration were significantl ...200112666977
periodontal microbiota and clinical periodontal status in a rural sample in southern thailand.we sought to determine (i) the association of subgingival bacterial profiles to clinical periodontal status in a population with limited access to dental care in thailand, and (ii) the external validity of our earlier findings from a similar study in rural china. we examined 356 subjects, 30-39 yr old and 50-59 yr old, with respect to clinical periodontal status and subgingival plaque at maximally 14 sites per subject. checkerboard hybridizations were used to analyse a total of 4343 samples. the ...200212664464
bactericidal activities of methoxyfluoroquinolones gatifloxacin and moxifloxacin against aerobic and anaerobic respiratory pathogens in serum.gatifloxacin (bristol-myers squibb) and moxifloxacin (bayer) are new methoxyfluoroquinolones with broad-spectrum activity against aerobic and anaerobic pathogens of the respiratory tract. in this investigation, we analyzed the bactericidal activity in serum over time of these antimicrobials against three aerobic (streptococcus pneumoniae, haemophilus influenzae, and staphylococcus aureus) and four anaerobic (peptostreptococcus micros, peptostreptococcus magnus, fusobacterium nucleatum, and prevo ...200312654663
acid-neutralizing activity during amino acid fermentation by porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum.acid-neutralizing activity during amino acid fermentation by washed cells of porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum was studied. when the washed cells of these strains were anaerobically incubated in the presence of aspartylaspartic acid or glutamylglutamic acid for p. gingivalis, aspartic acid for p. intermedia and glutamic acid for f. nucleatum at an initial ph of 5.0 or 5.5, the ph of the incubation mixtures rose toward neutral. f. nucleatum had the highes ...200312654101
fusobacterium nucleatum impairs serum binding to porphyromonas gingivalis biofilm.mouse immune sera obtained by immunization with fusobacterium nucleatum and then porphyromonas gingivalis demonstrated an impaired binding capacity to p. gingivalis-biofilm and lower avidity to p. gingivalis when compared with sera obtained from mice immunized with p. gingivalis alone.200312654097
helicobacter pylori and campylobacter rectus share a common antigen.aim: the aim of this study was to investigate the presence of antigens with immunological cross-reactivity in periodontopathogenic bacteria and helicobacter pylori, the pathogen associated with gastritis and peptic ulcers in human. materials and methods/results: among the putative periodontopathogens tested (actinobacillus actinomycetemcomitans, campylobacter rectus, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia and treponema denticola), cross-reactive bands were only ...200312654095
serum antibodies of periodontitis patients compared to the lipopolysaccharides of porphyromonas gingivalis and fusobacterium nucleatum.serum antibody titers against the lipopolysaccharides (lpss) of porphyromonas gingivalis and fusobacterium nucleatum were compared between 9 periodontitis patients and 24 healthy persons. the igg titers against the lpss of p. gingivalis atcc 33277(t) and w50 were clearly higher in the patients than in the healthy persons. however, igm titers against the lpss of p. gingivalis strains were relatively low, and no significant difference was observed between the patients and healthy persons. on the o ...200312636253
the reduction of fusobacterium nucleatum in mice is irrelevant to the nitric oxide induced by inos.previously we reported that mice infected recurrently with live fusobacterim nucleatum (fn) synthesize a significant amount of no between 12 hr and 24 hr after the fn injection. we now investigated whether the no has the capability of killing fn, a gram-negative rod periodontal pathogen. the mice were divided into three groups: treated with live bacteria (lb), treated with heat-killed bacteria (hkb) and untreated: normal (n). the fn reduction, no production and cell number after fn injection wer ...200312636250
bactericidal effects of different laser wavelengths on periodontopathic germs in photodynamic therapy.this study was an attempt to clarify whether the bactericidal effects of photodynamic therapy (pdt) are wavelength or dose-dependent. we also attempted to create an optimised protocol for a light-based bactericidal modality to eliminate periodontal pathogens. cultures of actinobacillus actinomycetemcomitans, fusobacterium nucleatum, porphromonas gingivalis, prevotella intermedia, and streptococcus sanguis, were exposed to a he-ne laser (632.8 nm) with a 30 mw power output, a 100 mw diode laser a ...200312627274
mass transport of macromolecules within an in vitro model of supragingival plaque.the aim of this study was to examine the diffusion of macromolecules through an in vitro biofilm model of supragingival plaque. polyspecies biofilms containing actinomyces naeslundii, fusobacterium nucleatum, streptococcus oralis, streptococcus sobrinus, veillonella dispar, and candida albicans were formed on sintered hydroxyapatite disks and then incubated at room temperature for defined periods with fluorescent markers with molecular weights ranging from 3,000 to 900,000. subsequent examinatio ...200312620862
faropenem, a new oral penem: antibacterial activity against selected anaerobic and fastidious periodontal isolates.the in vitro activity of faropenem, an oral penem, was compared with those of penicillin, co-amoxiclav, cefoxitin, clindamycin, erythromycin and metronidazole against 106 isolates of anaerobic pathogens involved in systemic infections. the organisms tested comprised porphyromonas gingivalis (29), prevotella spp. (eight), prevotella melaninogenica (seven), prevotella intermedia (five), actinomyces spp. (25), fusobacterium nucleatum (14), peptostreptococcus spp. (11), bacteroides ureolyticus (five ...200312615878
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