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leukemia inhibitory factor: does the cap fit? 19911906256
neuropeptide-y innervation of the rat spleen: another potential immunomodulatory neuropeptide.neuropeptide-y (npy) is a 36 amino acid peptide that acts as a chemical messenger in the central and peripheral nervous systems. npy often is found colocalized with the classical neurotransmitter norepinephrine (ne) and can potentiate the effects of this neurotransmitter postsynaptically in many systems. using immunocytochemistry for npy and specific lymphoid cell markers, we mapped the distribution of npy-positive nerve fibers in the rat spleen. npy-positive nerve fibers were present along the ...19911906353
protein-kinase-c-independent activation of arachidonate release and prostaglandin e2 formation in macrophages interacting with certain bacteria.certain bacterial species, of which we selected fusobacterium nucleatum, gardnerella vaginalis, peptostreptococcus anaerobius and propionibacterium acnes, were found to induce release of arachidonic acid in a dose- and time-dependent manner in mouse macrophages. the release of arachidonic acid showed a characteristic lag period of approximately 10 min and was accompanied by selective transformation into prostaglandin e2. bacteria killed by various methods caused a similar response, indicating th ...19911915341
isolation and purification of bacteriocin from prevotella intermedia (bacteroides intermedius).thirty-three out of 46 strains of prevotella intermedia (bacteroides intermedius) isolated from gingival deposits of patients with adult periodontitis possessed the ability to inhibit growth of p. intermedia strain atcc 25611, and 6 of the 33 showed strong inhibitory activity. thirteen isolates did not have the ability to inhibit the growth of p. intermedia strain atcc 25611. an inhibition factor from one of the isolates, p. intermedia th14 which had strong inhibition, was purified and character ...19911920011
control of enterococcus faecalis sex pheromone cad1 elaboration: effects of culture aeration and pad1 plasmid-encoded determinants.aeration of plasmid-free enterococcus faecalis strains resulted in an 8- to 16-fold decrease in sex pheromone cad1 activity in culture filtrates. levels of two unrelated pheromones, cpd1 and cam373, were unaffected by culture aeration. aeration also resulted in a decrease in the expression of conjugative transfer functions observed in cells containing pad1 trab mutations, verifying a link between trab function and pheromone "shutdown." tests with a series of pad1 mini-plasmids indicated that the ...19911924555
tetracycline resistance in peptostreptococcus species.of 15 peptostreptococcus sp. strains isolated between 1975 and 1984, 13 hybridized with the tet k, tet m, or tet o determinant. a donor peptostreptococcus anaerobius strain carrying the three determinants could transfer tet m to p. anaerobius and fusobacterium nucleatum recipients but not to an enterococcus faecalis recipient, while neither tet k nor tet o was transferred.19911929341
accumulation of plasma cells in inflamed sites: effects of antigen, nonspecific microbial activators, and chronic inflammation.plasma cells are common in chronically inflamed sites, including periodontal lesions. the aim of this study was to determine which factors contribute to this local accumulation of plasma cells. specifically, we sought to evaluate the effects of specific antigen and nonspecific activators from an infectious agent associated with chronic inflammation (fusobacterium nucleatum, an organism prominent in chronic periodontal lesions) and the effect of the chronic inflammation itself. chronic inflammati ...19911937760
reactivity of igg from explant cultures of periapical lesions with implicated microorganisms.the presence of igg in periapical inflammatory lesions suggests that immune responses participate in the disease process. the purpose of this investigation was to study the reactivity of igg from the supernatant fluids of explant cultures of periapical lesions with microorganisms implicated in infections of endodontic origin. ninety periapical lesions that had been contiguous with the apex of a root were removed and maintained in explant cultures. a dot-enzyme-linked immunosorbent assay (dot-eli ...19911940739
specificity of antibodies present in human periapical lesions.various classes of immunoglobulins have been found in human periapical lesions. the specificity of secreted antibodies against antigens egressing from the root canal system has yet to be thoroughly investigated. the purpose of this study was to test the specificity of antibodies present in human periapical lesions. human periapical biopsies were removed and cultured as organ culture explants. antibodies present in the lesions were extracted in the cell culture fluids. a modified enzyme-linked im ...19911940740
preliminary investigation of crystals of the neutral lipase from pseudomonas fluorescens.the neutral lipase from the bacteria pseudomonas fluorescens, marketed under the trade name lpl-200s, has been crystallized in a form suitable for x-ray diffraction analysis from 35% n-propanol at ph 8.5. the crystals are monoclinic prisms and are of space group c2 with a = 91.00 a, b = 47.17 a, c = 35.21 a and beta = 121.43 degrees. there is one molecule of the protein as the asymmetric unit of the crystals. the diffraction pattern extends to at least 1.6 a resolution and the crystals are extre ...19911942065
dna probe detection of eikenella corrodens, wolinella recta and fusobacterium nucleatum in subgingival plaque.this cross-sectional study used species-specific dna probes to examine subgingival plaque specimens for the presence of eikenella corrodens, wolinella recta, and fusobacterium nucleatum in adults with untreated periodontitis or gingivitis and in healthy controls. w. recta and f. nucleatum were more prevalent in diseased sites from the periodontitis group when compared with the controls (81% vs 22% and 83% vs 20% respectively). e. corrodens was detected in 62% of the control sites and 81% of the ...19911945492
quantitative analysis of the epstein-barr virus-inducing properties of short-chain fatty acids present in the culture fluids of oral bacteria.the culture fluids of various anaerobic bacteria induced the synthesis of early antigens (ea) in epstein-barr virus (ebv) carrying lymphoblastoid cells. the culture fluids of corynebacterium butyricum and fusobacterium nucleatum were the effective inducer on ea. the inducing activity was, to some extent, dependent on their n-butyric acid content, but appeared to be regulated by yet unidentified materials.19911652240
subgingival microflora of advanced periodontitis in the dominican republic.a study of the predominant subgingival microflora was carried out in 24 periodontitis patients, 18 to 60 years of age, in santo domingo, dominican republic. paper point sampling, transport in vmga iii, and conventional microbiological techniques were utilized. direct microscopic examination revealed that cocci and nonmotile organisms made up 85% of the total organisms and spirochetes as little as 3%. nonselective culturing showed gram-negative organisms to constitute 53% of total isolates. fusob ...19911658290
relation of baseline microbial parameters to future periodontal attachment loss.the relationship between the level of subgingival species at baseline and subsequent attachment loss in a subject was examined. 38 subjects (14-71 years) with prior evidence of periodontal destruction were monitored 2x for pocket depth and attachment level at 6 sites per tooth at baseline and 2 months. a subject was considered to exhibit new attachment loss if 1 or more sites increased 3 mm or more in attachment level in 2 months. subgingival plaque samples were taken at the baseline visit from ...19911661304
systemic antibody responses to oral microorganisms in the cynomolgus monkey: development of methodology and longitudinal responses during ligature-induced disease.systemic antibody responses to oral microorganisms were studied during ligature-induced periodontal disease in a non-human primate (nhp) model. methodology was developed using elisa techniques to assess total igg and igm levels in the serum from the nhp. in addition, an elisa was developed utilizing affinity-purified anti-human isotype reagents to detect nhp serum antibody responses to porphyromonas gingivalis, actinobacillus actinomycetemcomitans, prevotella intermedia and fusobacterium nucleat ...19911665573
phenotypic analysis of b-cells extracted from human periodontal disease tissue.b-cells extracted from periodontal disease tissue were analyzed for the presence of activation markers using a range of monoclonal antibodies. in adult periodontitis (ap), 6% of b-cells expressed the il-2 receptor (cd25) compared with 1-2% in peripheral blood and healthy or marginal gingivitis (h/mg) gingival b-cells. there was also an increase in the mean percentage of igd-positive b-cells and a decrease in cd21 and cd22 expression. in both ap and h/mg lesions, 20-22% of the b-cells expressed c ...19911668249
[severe infections due to streptococcus milleri in children].streptococcus milleri (s. milleri) is found in healthy individuals in the mouth, nasopharynx, throat, vagina and in feces, and has been reported to be isolated from several infectious diseases in man, particularly from abscess in various parts of the body. we report two cases of severe infections due to s. milleri isolated from subdural abscess and pleural empyema. [case 1] 13 year old boy who had been healthy until he was noticed to have meningeal signs and was diagnosed as left subdural absces ...19911680938
peptidoglycan precursor from fusobacterium nucleatum contains lanthionine.fusobacterium nucleatum was grown in the presence of [14c]udp. by means of sequential precipitation and chromatographic separation of the cytoplasmic content, a peptidoglycan [14c]udp pentapeptide containing lanthionine was isolated. this finding indicates that lanthionine is synthesized and incorporated as such during the assembly of the peptidoglycan.19911987169
microbiological and immunological aspects of experimental periodontal disease in rats: a review article.animal models in which microbiological and immunological aspects of periodontal disease can be studied prospectively seem well warranted. the rat bears much resemblance to man with respect to periodontal anatomy, development and composition of dental plaque, histopathology of periodontal lesions, and basic immunobiology. furthermore, reproducible methods are available for assessment of periodontal disease in rats, and detectable periodontal destruction can be induced in a few weeks in these anim ...19912002433
the helicobacter (campylobacter) pylori-colonized duodenal mucosa and gastric metaplasia.biopsies were obtained from non-ulcerated sites of the duodenum from 100 dyspeptic patients. helicobacter (campylobacter) pylori was cultivated from 19 of these biopsies. active chronic duodenitis (acd) was found in 17 biopsies and more than 5% gastric metaplasia in 20 biopsies. h. pylori as well as acd occurred with a significantly increased frequency when more than 5% gastric metaplasia was found in the duodenal biopsies. h. pylori on metaplastic tissue without acd was, however, seen in two ca ...19912018637
a randomized, placebo-controlled trial of doxycycline: effect on the microflora of recurrent periodontitis lesions in high risk patients.twenty-seven patients with a recent history of periodontal abscesses and/or loss of gingival attachment level (gal) despite active periodontal therapy were enrolled in a double-blind, randomized, placebo-controlled trial. clinical measurements and subgingival scaling were performed every 2 months. when a site exhibited greater than or equal to 2 mm loss of gal or a periodontal abscess, patients were administered either doxycycline at a dosage of 200 mg to start and 100 mg per day for 3 weeks, or ...19912027071
[right-sided endocarditis due to fusobacterium nucleatum].we present 1 case of right sided endocarditis caused by fusobacterium nucleatum in a patient with intravenous drug addiction and human immunodeficiency. the clinical features were fever, anemia, and pulmonary embolism. the echocardiogram showed a giant vegetation originated from the right atrial wall prolapsing in diastole into the right ventricle which disappeared after the patient presented pulmonary embolism. the clinical course was uncontrolled with empiric antimicrobial therapy but it was g ...19912047551
purification and characterization of a 65-kilodalton diisopropylfluorophosphate-binding protein in the outer membrane of fusobacterium nucleatum fev1.a 65-kilodalton protein was identified in the outer membrane of fusobacterium nucleatum fev1 by sds-page. the relative amount of the protein varied during growth, being greatest in stationary phase. the protein was radio-labeled by [125i]-lactoperoxidase treatment of live cells and was only partially extractable with 2% sodium dodecylsulfate (sds) at room temperature, and therefore assumed to be both exposed to the cell surface and peptidoglycan associated. in intact cells the protein bound diis ...19912047749
amoxycillin with clavulanic acid and tetracycline in periodontal therapy.the effects of tetracycline and amoxycillin with clavulanic acid on the clinical parameters and subgingival flora of eight patients with rapidly progressive periodontitis was assessed. subjects received either tetracycline 250 mg four times daily or amoxycillin 250 mg with clavulanic acid 125 mg three times daily for a period of 2 weeks together with subgingival scaling and root planning. both treatment regimens produced significant reductions in bleeding on probing and probing pocket depths whi ...19912050897
synergistic effect on blastogenesis in murine spleen cells of lipopolysaccharide, lipid a, and acid-degraded polysaccharide from fusobacterium nucleatum.interchangeable combinations of fusobacterium nucleatum fev1 lipopolysaccharide (lps) with its split products by acetic acid hydrolysis, i.e. lipid a (la) and degraded polysaccharide (ps), amplified the blastogenic response in murine spleen cell cultures as measured by [3h]thymidine uptake. athymic murine spleen cells precultured with lps-fev1 for 48 h (stage 1), washed twice and cultured together with fresh cells and either la or ps for 72 h (stage 2) gave a synergistic response over that found ...19912059480
intrageneric relationships of members of the genus fusobacterium as determined by reverse transcriptase sequencing of small-subunit rrna.the phylogenetic interrelationships of 14 members of the genus fusobacterium were investigated by performing a comparative analysis of the 16s rrna sequences of these organisms. the sequence data revealed considerable intrageneric heterogeneity. the four species fusobacterium nucleatum (including f. nucleatum subsp. nucleatum, f. nucleatum subsp. polymorphum, "f. nucleatum subsp. fusiforme," and "f. nucleatum subsp. animalis"), fusobacterium alocis, fusobacterium periodonticum, and fusobacterium ...19911715737
the association of occult amniotic fluid infection with gestational age and neonatal outcome among women in preterm labor.to evaluate the relationships between gestational age, neonatal outcome, and amniotic fluid (af) bacteria, we obtained af from women with intact membranes in idiopathic preterm labor. positive cultures were obtained from 20 (19%) of 105 women. the frequency of positive cultures was inversely related to gestational age: 23-26 weeks, nine of 20; 27-30 weeks, four of 24; and 31-34 weeks, seven of 61 (chi2 for trend, p less than .001). fusobacterium nucleatum, bacteroides ureolyticus, and ureaplasma ...19921738513
fusobacterium nucleatum pyomyositis. 19921738725
dofetilide, a novel class iii antiarrhythmic agent.dofetilide is a potent and selective class iii antiarrhythmic agent that is under development for the treatment of re-entrant tachyarrhythmias (ventricular tachycardia/ventricular fibrillation, atrial fibrillation/atrial flutter, and paraoxysmal supraventricular tachycardia). in animal studies, dofetilide selectively inhibits the rapid component of the time-dependent outward potassium current (ikr) and therefore increases the effective refractory period and action potential duration without affe ...19921279316
development and characterization of porphyromonas gingivalis-specific rat t-cell clones.porphyromonas gingivalis has been implicated as a major pathogen in periodontitis. to determine the role of t cells in the regulation of this disease, a method was developed for the generation and characterization of rat t-cell clones with antigen specificity to p. gingivalis whole cells. the clones studied so far demonstrated a t-helper (th) phenotype w3/13+, w3/25+, ox8- and ox22-. these t-cell clones proliferated in vitro in response to p. gingivalis, but not to other bacteria (prevotella int ...19921281976
regeneration of an adult peripheral nerve preparation in culture.the methods used to maintain the vagus nerve from the adult rat in culture and how regeneration is studied in this preparation are described. a hypothesis is presented on the triggering of the cell body reaction. it is suggested that this reaction is initiated by proteins synthesized in nonneuronal cells at the site of a nerve lesion. these proteins, referred to as regenerins, reach the nerve cell body by retrograde axonal transport, where they initiate the regeneration process.19921282333
[treatment and monitoring of primary hypothyroidism].treatment of primary hypothyroidism requires a long-term hormonal therapy with levothyroxine which restores a normal tsh value. in a series of 192 patients followed up for at least 3 years we tried to evaluate the variability of maintenance dosage and to analyse the factors predictive of replacement dosage. the l-t4 dosage varied from one individual to the other, from 0.6 to 3.2 micrograms/kg. the mean maintenance dosage (118 +/- 38 micrograms/day) was similar in men and women weight for weight, ...19921287777
myocardial protection by retrograde cardioplegic perfusion in the presence of acute coronary artery obstruction. an experimental study.to investigate retrograde delivery of cardioplegic solutions as a means of enhancing myocardial protection in the presence of coronary artery occlusion, a two-part experimental model was devised. in part 1 (in vitro) the possibility of retroperfusing the entire myocardium during acute occlusion of the left anterior descending artery (lad) was assessed. in part 2 (in vivo) acute lad occlusion was performed in dogs, and during 2 hours of aortic cross-clamping crystalline cardioplegic solution was ...19921287835
soft-tissue management through effective patient subgingival bacterial control. 19921291435
expression and release of neuroregulators during development: monoamines and neuropeptides.the turnover of catecholamines (ca) was determined in the adrenal medulla and brain of rat fetuses and pups. in general we found a considerable increase soon after birth. the expression of mrna for ca-synthesizing enzymes was also considerably enhanced in the adrenals shortly after birth. furthermore, we demonstrated increased expression of neuropeptides after birth, increased synthesis of mrna encoding for neuropeptide y in the adrenals 24 h after birth; and considerable activation of the subst ...19921306802
hypoxic-ischemic damage in the neonatal brain: excitatory amino acids.perinatal brain damage is a major clinical problem. recent studies suggest that excitatory amino acids (eaas) may be important for the development of hypoxic-ischemic brain injury in the newborn. experimental work demonstrates that the immature brain is hypersensitive to the toxic effects eaa ('excitotoxicity'), hypoxic-ischemia is accompanied by an extracellular overflow of eaas and hypoxic-ischemic brain damage is reduced by eaa receptor antagonists. clinical investigations demonstrate the pre ...19921306803
the effect of 3 mouthrinses on plaque and gingivitis development.the aim of this study was to evaluate the effect of 3 mouthrinses, listerine antiseptic (thymol), peridex (chlorhexidine), perimed (povidone iodine and hydrogen peroxide), and a placebo (water) on the development of dental plaque and gingivitis, when used as the only oral hygiene procedure for 14 days. 71 subjects were entered into a randomized, double-blind study. at the baseline examination, papillary bleeding score (pbs), and plaque index (pi) were registered, after which subjects received su ...19921310096
microbiology of healthy and diseased periodontal sites in poorly controlled insulin dependent diabetics.a group of poorly-controlled insulin dependent diabetes mellitus (iddm) patients were examined in a cross-sectional design for total microbial levels, microbial incidence, and the percent levels of selected periodontal microorganisms. these organisms were selected on the basis of prior reports that associated them with either periodontal disease or health. one periodontally-healthy and one periodontally-diseased site were examined in each iddm patient. increased levels of the periodontal pathoge ...19921315389
use of adeno-associated virus as a general transduction vector for mammalian cells. 19921316261
analyzing phenotypic correlations in studies with selected lines. 19921317160
the use of dna probes to examine the distribution of subgingival species in subjects with different levels of periodontal destruction.the present investigation examined the distribution of 14 subgingival species at a total of 2299 sites in 90 subjects with different levels of periodontal destruction. subgingival plaque samples taken from the mesial aspect of each tooth were anaerobically dispersed, diluted and plated on non selective media. after anaerobic incubation, colonies were lifted to nylon filters and specific species detected using digoxygenin-labeled whole chromosomal dna probes. the mean total viable count for all s ...19921318331
new monoclonal antibodies for the detection of immediate early antigens of cytomegalovirus.two new monoclonal antibodies, cie-1 and cie-2, were developed for the rapid detection of human cytomegalovirus (hcmv) infection. they were found to be reactive with immediate early protein of hcmv in the nuclei of infected fibroblasts, as early as 3 hours post-infection. by radioimmunoprecipitation, cie-1 was found to react with a protein with an apparent molecular weight of 70,000, whereas cie-2 precipitated 2 proteins of 70,000 and 72,000 daltons, respectively. both monoclonal antibodies reco ...19921319172
subgingival temperature (iii). relation to microbial counts.the present investigation examined the relationship of selected bacterial species and subgingival temperature. 35 subjects were measured at 6 sites per tooth for clinical parameters and subgingival temperature. measurements were repeated for 21 subjects at 2 month intervals providing a total of 66 subjects visits. at each visit, subgingival plaque samples were taken from the mesial aspect of each tooth and anaerobically dispersed, diluted and plated on non-selective media. after anaerobic incuba ...19921321846
synergistic effect of pyrophosphate and sodium dodecyl sulfate on periodontal pathogens.our previous studies have shown that pyrophosphate (ppi), the anticalculus component of tartar-control dentifrices, inhibits the growth of organisms associated with coronal and root surface caries. the purposes of this investigation were to: 1) determine if periodontal pathogens are similarly susceptible to the growth-inhibitory properties of ppi; and 2) determine if combinations of pyrophosphate-sodium dodecyl sulfate (ppi-sds) inhibit growth synergistically. porphyromonas gingivalis, actinobac ...19921324304
role of porphyromonas gingivalis 40-kda outer membrane protein in the aggregation of p. gingivalis vesicles and actinomyces viscosus.porphyromonas gingivalis, an important pathogen in periodontitis, produces extracellular vesicles that aggregate with actinomyces viscosus cells. a 40-kda outer membrane protein (omp)-coding gene from p. gingivalis was cloned and the protein was found to be localized in these vesicles. the recombinant 40-kda omp did not show aggregation activity. however, affinity-purified antibody against the recombinant protein significantly inhibited aggregation of p. gingivalis vesicles with a. viscosus cell ...19921329700
effect of clavulanic acid, sulbactam and tazobactam on three different beta-lactamases from bacteroides uniformis, clostridium butyricum and fusobacterium nucleatum.the effect of three beta-lactamase inhibitors, clavulanic acid, sulbactam and tazobactam used in clinical practice were compared for their activity against purified beta-lactamases from bacteroides uniformis, clostridium butyricum and fusobacterium nucleatum. the enzymes from b. uniformis and c. butyricum were produced in fermenters under controlled growth conditions and the enzyme from f. nucleatum was produced in batch cultures. purification of the beta-lactamases was achieved by anion-exchang ...19921331018
correlation of haemagglutination activity with trypsin-like protease activity of porphyromonas gingivalis.porphyromonas gingivalis is a gram-negative anaerobic bacterium associated with various forms of periodontal disease. several characteristics of p. gingivalis are thought to contribute to its pathogenicity; these include haemagglutination and trypsin-like protease activity. previous studies suggest an association between haemagglutination and trypsin-like protease activity of p. gingivalis. to investigate this, two complementary quantitative experimental approaches were taken. five independent m ...19921332663
sensitization of oral bacteria in biofilms to killing by light from a low-power laser.biofilms of streptococcus sanguis, porphyromonas gingivalis, fusobacterium nucleatum and actinobacillus actinomycetemcomitans were prepared on the surfaces of agar plates and a number of compounds were screened for their ability to sensitize bacteria in these biofilms to killing by light from a 7.3 mw helium/neon (he/ne) laser. toluidine blue o and methylene blue enabled detectable killing of all four target organisms after exposure to he/ne light for 30 s. aluminium disulphonated phthalocyanine ...19921334649
bacterial concentration fluorescence immunoassay (bcfia) for the detection of periodontopathogens in plaque.a bacterial concentration fluorescence immunoassay (bcfia) was developed to rapidly detect periodontopathic bacteria in human plaque samples. the bcfia utilized fluorescent-tagged monoclonal antibodies (mabs) directed against the lipopolysaccharide of selected gram-negative bacteria. microorganisms identified in plaque using the bcfia included actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, and fusobacterium nucleatum. the immunoassay procedure involved com ...19921336047
dna probe and enzyme analysis in periodontal diagnostics.recent research in periodontology has indicated that a finite number of bacterial species are associated with periodontal disease. this has generated the hypothesis that periodontal disease is essentially an infection due to one or more of the putative periodontopathogens; i.e., the specific plaque hypothesis (sph). this microbial specificity paradigm has already changed the way researchers and some clinicians view periodontal disease. the clinician must heed several cautions signs, however, if ...19921336048
antibacterial efficacy of root canal medicaments.the antibacterial efficacy of four endodontic medicaments was studied in vitro. calcium hydroxide (calacept), camphorated parachlorophenol (cpcp), cresophene, and 2% iodine-potassium iodide (iki) were tested against four anaerobic microorganisms, streptococcus mutans, peptostreptococcus anaerobius, porphyromonus gingivalis and fusobacterium nucleatum, for 10 or 15 min. iki was effective only against f. nucleatum and p. gingivalis, and the others were effective against all four microorganisms.19921337555
effect of beta-lactamase inhibitors on beta-lactamases from anaerobic bacteria.three beta-lactamase inhibitors in clinical use--clavulanic acid, sulbactam and tazobactam--were investigated for their activity on beta-lactamases from bacteroides uniformis, clostridium butyricum and fusobacterium nucleatum. purification of the beta-lactamases was carried out by anion-exchange chromatography, gel filtration and fplc. the inactivation of beta-lactamase activity was determined spectrophotometrically with nitrocefin as substrate. various concentrations of the inhibitors were prei ...19921338315
release of endogenous noradrenaline from the vascularly perfused rat stomach in vitro: modulation by pre- and postsynaptic adrenoceptors.we developed an experimental in vitro model to detect a very small amount of endogenous noradrenaline (na) released from the rat gastric sympathetic nerve terminals. the stomach was perfused via celiac artery with modified krebs-ringer solution containing 10 mm pargyline and 0.1% bovine serum albumin at a constant flow of 4 ml/min. the right greater splanchnic (spl) nerve (preganglionic nerve of the gastric sympathetic nerve) was stimulated electrically with square-wave pulses of 2 msec duration ...19921346643
structure of holo-chaperonin studied with electron microscopy. oligomeric cpn10 on top of two layers of cpn60 rings with two stripes each.a structural model of holo-chaperonin, known as a protein-folding control protein comprising 60 kda (cpn60) and 10 kda polypeptides (cpn10), is proposed based on the electron microscopic images of holo-chaperonin from thermus thermophilus and cpn60 from paracoccus denitrificans. isolated paracoccus cpn60 shows very similar images to those of escherichia coli tetradecameric cpn60, a seven-membered ring in the top view and a rectangular shape with four stripes in the side view. however, a small nu ...19921347504
detubularization-induced contractile response change of the ileum following ileocystoplasty.we reported previously that following ileocystoplasty the structure and pharmacologic response of the implanted ileum changes towards that of the bladder. specifically, the relaxation response to alpha adrenergic (methoxamine) and purinergic (atp) stimulation reverses to a contractile response one month after the ileal segment is surgically made part of the urinary bladder. the present study was designed to investigate possible signals for this change and also to determine whether bladder respon ...19921351953
immunoglobulins in milk from cows immunized with oral strains of actinomyces, prevotella, porphyromonas, and fusobacterium.immunization of pregnant cows with bacteria leads to the presence of high concentrations of specific antibodies in colostrum and milk. a total of 14 cows was immunized with single strains of heat-killed oral bacteria or pools of strains of actinomyces, porphyromonas, prevotella, and fusobacterium. two cows were treated with adjuvant alone. the mean percentages of igg1, igg2, igm, and iga in all of the milks were 83.8, 3.8, 9.3, and 3.1, respectively. elisa and whole cell agglutination assays dem ...19921354674
cd45ra and cd45ro positive cd4 cells in human peripheral blood and periodontal disease tissue before and after stimulation with periodontopathic bacteria.flow cytometric analysis was used to examine naive and primed or memory cd4 cells extracted from periodontal lesions compared with cells from peripheral blood of healthy subjects before and after stimulation with the periodontopathic bacteria, porphyromonas gingivalis and fusobacterium nucleatum. in peripheral blood, approximately 60% and 40% of cd4 cells were cd45ro+ and cd45ra+ respectively at day 0. phytohaemagglutinin (pha) induced cd45ro expression on almost 100% of cd4 cells. however, p. g ...19921356262
different responses in b cells induced by porphyromonas gingivalis and fusobacterium nucleatum.a phenotypic study had shown that gingival b cells respond differently to two periodontopathic bacteria, porphyromonas gingivalis and fusobacterium nucleatum. further investigation now shows a reduction in the percentage of ki-67 + t cells in cultures of gingival and peripheral blood mononuclear cells stimulated with p. gingivalis for 3 and 6 days, respectively, but no suppression of ki-67 expression in b cells in response to either p. gingivalis or f. nucleatum. depletion studies of cultures of ...19921359860
sensitization of oral bacteria to killing by low-power laser radiation.twenty-seven compounds were screened for their ability to sensitize streptococcus sanguis to killing by light from a 7.3-mw helium/neon (hene) laser. bacteria were mixed with various concentrations of the test compounds, spread over the surfaces of agar plates, and then exposed to light from the hene laser for various time periods. the plates were then incubated and examined for zones of inhibition. those compounds found to be effective photosensitizers were then tested against porphyromonas gin ...19921369193
characterization of guanosine diphospho-d-mannose dehydrogenase from pseudomonas aeruginosa. structural analysis by limited proteolysis.alginate is believed to be a major virulence factor in the pathogenicity of pseudomonas aeruginosa in the lungs of patients suffering from cystic fibrosis. guanosine diphospho-d-mannose dehydrogenase (gdpmannose dehydrogenase, ec 1.1.1.132) is a key enzyme in the alginate biosynthetic pathway which catalyzes the oxidation of guanosine diphospho-d-mannose (gdp-d-mannose) to gdp-d-mannuronic acid. in this paper, we report the structural analysis of gmd by limited proteolysis using three different ...19921370473
dinucleotide (ca/gt) repeat polymorphism in intron 17b of the cystic fibrosis transmembrane conductance regulator (cftr) gene.we describe a polymorphic microsatellite (ivs17bca) in intron 17b of the cystic fibrosis transmembrane conductance regulator (cftr) gene. it consists of an 11 to 20 ca/gt dinucleotide repeat, located 424 bp from exon 17b.19921370810
biochemical and functional characterization of the leucocyte tyrosine phosphatase cd45 (cd45ro, 180 kd) from human neutrophils. in vivo upregulation of cd45ro plasma membrane expression on patients undergoing haemodialysis.the biochemical and functional characterization, and the regulation of plasma membrane expression of the leucocyte tyrosine phosphatase cd45, have been investigated in neutrophils from healthy donors and patients undergoing haemodialysis. cd45 proteins of 180 kd and 130-150 kd were precipitated from neutrophils from both healthy subjects and haemodialysed patients. prolonged storing, as well as trypsin treatment of samples containing the 180-kd cd45 protein, generated the 130-150-kd polypeptides ...19921370931
the establishment of human t cell lines reactive with specific periodontal bacteria.human t cell lines (tcls) were obtained by stimulation of peripheral blood mononuclear cells (pbmcs) with the 2 periodontopathic bacteria, porphyromonas gingivalis fdc-381 and fusobacterium nucleatum fdc-263. after the first round of stimulation and rest, the cells responded specifically to the bacteria originally used to establish each line. throughout the culture period, the responsiveness of each of the tcls to their specific bacteria increased. phenotypic analysis of the tcls revealed hetero ...19921383912
[lemierre's syndrome with splenic abscesses].a week after onset of a pharyngo-tonsillitis a previously healthy 23-year-old man developed high fever (41.4 degrees c), leukocytosis (12,200/microliters) with marked shift to the left, thrombocytopenia (86,000/microliters) and increased transaminases (got 83 u/l, gpt 113 u/l). chest x-ray film suggested intrapulmonary abscesses with left-sided pleural effusion. the suspected diagnosis of "post-tonsillitis" septicaemia (lemierre's syndrome) was confirmed by demonstrating anaerobic, fusiform, gra ...19921396137
identification of proteases from periodontopathogenic bacteria as activators of latent human neutrophil and fibroblast-type interstitial collagenases.activation of latent human fibroblast-type and neutrophil interstitial procollagenases as well as degradation of native type i collagen by supra- and subgingival dental plaque extracts, an 80-kda trypsinlike protease from porphyromas gingivalis (atcc 33277), a 95-kda chymotrypsinlike protease from treponema denticola (atcc 29522), and selected bacterial species commonly isolated in periodontitis was studied. the bacteria included were prevotella intermedia (atcc 25261), prevotella buccae (es 57) ...19921398963
longitudinal monitoring of humoral antibody in subjects with destructive periodontal diseases.fifty-one subjects (16-61 years old) with evidence of prior destructive periodontal disease were monitored clinically and immunologically at bi-monthly intervals for up to 5 yr. periodontal disease activity, determined as new attachment loss, was detected in 33 of these subjects. only 4 of 51 subjects failed to show an elevated serum antibody level to any of the 18 subgingival species tested. the antibody level threshold established for periodontally healthy subjects was exceeded most often in d ...19921403580
experimentally induced infection by oral anaerobic microorganisms in a mouse model.a mouse model was developed to study the abscessogenic potential of pure and mixed cultures of oral anaerobes associated with infections of endodontic origin. maximum subcutaneous lesions were seen on their backs between 3 and 14 d after inoculation. strains of fusobacterium nucleatum, peptostreptococcus anaerobius, and veillonella parvula were pathogenic in pure culture. a mixed culture of f. nucleatum with either porphyromonas gingivalis or prevotella intermedia was significantly more pathogen ...19921408362
2-hydroxyglutaryl-coa dehydratase from fusobacterium nucleatum (subsp. nucleatum): an iron-sulfur flavoprotein.anaerobically prepared cell-free extracts from fusobacterium nucleatum contain 2-hydroxyglutaryl-coa dehydratase with a specific activity of 20 nkat mg-1. the enzyme was purified 24-fold to a specific activity of 480 nkat mg-1 by anion exchange chromatography, gel filtration and chromatography on blue-sepharose. the activity of the purified enzyme was strictly dependent on the reductant ti(iii)citrate and stimulated 25-fold by 0.15 mm atp and 5 mm mgcl2. atp is hydrolysed to adp during incubatio ...19921417419
mannitol does not affect energy metabolism in forebrain ischemia.mannitol has a beneficial effect on ischemic injury following a short-duration forebrain ischemic insult in rats. using the same animal model, we attempted to show that this effect of mannitol could be traced (via an improvement in cerebral blood flow) to a tempering of the collapse in the high-energy phosphates that occurs during the insult. a 10 min ischemic insult was induced by bilateral carotid artery occlusion followed by a reduction of the blood pressure to 50 mmhg through removal of bloo ...19921421096
complement activation by lipopolysaccharides purified from gram-negative bacteria isolated from infected root canals.the purpose of the present study was to investigate the complement activation by lipopolysaccharides purified from porphyromonas (bacteroides) endodontalis, veillonella parvula, and fusobacterium nucleatum isolated from infected root canals. the rate of consumption of the c3t component of the complement increased remarkably when lipopolysaccharides of more than 10 micrograms were added.19921437068
comparison of the effects of galactose and glucose on the ph responses of human dental plaque, salivary sediment and pure cultures of oral bacteria.comparisons made in dental plaque in vivo demonstrated that galactose produces a significantly smaller decrease in ph than does glucose. in vitro studies with plaque, salivary sediment and pure cultures of oral bacteria done in the absence of intraoral factors such as flowing saliva confirmed this lesser acidogenicity of galactose. pure culture showed that most of the bacteria tested produce a moderate to large decrease with glucose but only a few do so with galactose; most produced a moderate t ...19921444892
a comparative study of randomly amplified polymorphic dna analysis and conventional phage typing for epidemiological studies of listeria monocytogenes isolates.the analysis of rapd profiles generated by pcr with a single 10-mer, hlwl74, was compared to bacteriophage susceptibility data for epidemiological typing of listeria monocytogenes strains. a total of 104 l. monocytogenes strains was screened, all from serogroup 1 or serotype 4b. of these, 53 had been isolated during 6 different listeriosis outbreaks. the remaining 51 strains were chosen randomly from our collection. a total of 38 rapd types were observed, although each epidemic group of strains ...19921448626
immunohistochemical localization of gangliosides in enu-induced rat glioma.biochemical studies have indicated that the structurally simple gangliosides, including gd3 and gm3, are major glycolipid components of glioma tissues. in order to clarify the localization of the gangliosides in ethylnitrosourea-induced rat glioma, an immunohistochemical study was performed using antibodies against gm1, gm3, and gd3. the results obtained in normal fetus, newborn, and adult rat brain, and also in human glioma, were compared. in fetal and newborn rat brain, gd3 was present mainly ...19921449052
an unrecognized etiology for pyogenic hepatic abscesses in normal hosts: dental disease.cryptogenic pyogenic hepatic abscesses are a diagnosis of exclusion. we have identified two patients with severe dental disease at the time of the diagnosis of their liver abscess. in both cases, oral flora was cultured from the abscess. unlike a previous report, both patients were immunocompetent. when compared with a group of patients with liver abscesses and diverticulitis, two differences were found. in contrast to the single abscesses seen in 10 of 10 patients with diverticulitis, the patie ...19921449134
cellular fatty acids in fusobacterium species as a tool for identification.identification of fusobacteria from clinical specimens currently requires analysis of metabolic end products by gas-liquid chromatography in addition to certain biochemical and enzymatic tests because of the relative biochemical inactivity of these bacteria. even the finding of pointed, thin gram-negative cells on gram-stained slides can no longer be relied on for identification of fusobacterium nucleatum, since at least four other species of fusobacteria have been seen to exhibit similar morpho ...19921452706
phenolic constituents in erythrina x bidwilli and their activity against oral microbial organisms.five flavonoid compounds, including two new isoflavanones, were isolated from the root bark of erythrina x bidwilli. their structures were determined to be 2,10-di(gamma,gamma-dimethylallyl)-3,9-dihydroxypterocarpan (erythrabyssin ii), 6,8-di(gamma,gamma-dimethylallyl)-7,2',4'-trihydroxyisoflavanone (bidwillon a), 8-gamma,gamma-dimethylallyl-2',4'-dihydroxy-[6",6"-di-methylpyrano - (2",3":7,6)]isoflavanone (bidwillon b), 8-gamma,gamma-dimethylallyl-7,4'-dihydroxyisoflavone (8-gamma,gamma-dimethy ...19921464104
clonal dental pulp cells (rdp4-1, rpc-c2a) synthesize and secrete osteopontin (spp1, 2ar).dental pulp cells play an important role in maintaining dental mineralized tissue throughout life. supplementary mineralization such as reparative dentin and pulp stone frequently occurs after primary dentin formation. dental pulp cells are thought to be closely associated with such mineralization. we found that clonal rat dental pulp cells, rdp4-1 and rpc-c2a, produce and secrete osteopontin, but do not synthesize phosphophoryn which is a major noncollagenous protein found in dentin. the dental ...19921472061
rat sertoli and spermatogenic cells express a similar gene, and its product is antigenically related to an outer dense fiber-associated protein.we have previously reported that a heterodimeric protein secreted by rat sertoli cells is antigenically related to a protein associated with outer dense fibers of the sperm tail. therefore, we have explored the possibility that sertoli and spermatogenic cells express a similar gene encoding a homologous protein. a sertoli cell heterodimeric protein cdna probe recognizes specific mrna in pachytene and round spermatids fractionated by centrifugal elutriation; however, this specific mrna was less p ...19921472368
hiv education and health education in the united states: a national survey of local school district policies and practices.to determine the extent to which hiv education and health education policies and practices are required by school districts in the united states, a national probability sample of public school districts was surveyed by mail in 1990. of 2,150 districts selected, 78.1% responded. hiv education was required by 66.9% of districts. of these, the percentage requiring hiv education increased by grade level from 29.7% in kindergarten to 82.3% in 7th grade, then declined to 37.3% by 12th grade. districts ...19921479838
[pneumococcus-induced septicemia in normal and splenectomized rabbits].a new rabbit model to study the consequences of splenectomy in host resistance to induced pneumococcal septicaemia is presented. a simple, fast, non-invasive and complication-free technique of bacterial inoculation into the rabbit airways, using transcutaneous cannulation of the trachea, is also described. sixteen normal and 24 splenectomised animals were used. the optimal dose of pneumonococcus (serotype b, type iii) was found to be 1 x 10(7) organisms. the above dose of bacteria given transtra ...19921479858
susceptibility of anaerobic microorganisms to hypothiocyanite produced by lactoperoxidase.the susceptibility of capnocytophaga ochracea, eikenella corrodens, eubacterium yurii, fusobacterium nucleatum, peptostreptococcus micros, prevotella intermedia, selenomonas sputigena, wolinella recta to hypothiocyanite (oscn-) produced by the lactoperoxidase system was tested. results showed a decrease of bacterial survival rate after oscn- exposure, with an intra- and inter-species variability from 0 to 95% for c. ochracea, 34-100% for e. corrodens, 0-83% for e. yurii, 1-15% for f. nucleatum, ...19921481764
expression of small heat shock proteins by the third-stage larva of brugia pahangi.changes in proteins synthesised by the infective third-stage larvae (l3) of the filarial nematode brugia pahangi were examined with respect to the temperature shift encountered by the parasite as it migrates from insect to mammal, and the presence of serum in the culture medium. while the synthesis of a number of polypeptides is regulated by the temperature shift of the l3 from 28 degrees c to 37 degrees c in vitro, there is no evidence that serum has any significant effect on protein synthesis. ...19921484547
associations between microbial species in dental root canal infections.the existence of commensal or antagonistic relationships between microorganisms in the root canals of teeth with apical periodontitis was investigated. samples were taken from 65 infected human root canals and were analysed according to species, frequency of occurrence and proportion of the total isolated flora. the most frequent species were fusobacterium nucleatum, prevotella intermedia, peptostreptococcus micros, peptostreptococcus anaerobius, eubacterium alactolyticum, eubacterium lentum and ...19921494447
relationship between oral gram-negative anaerobic bacteria in saliva of the mother and the colonization of her edentulous infant.various gram-negative anaerobic bacterial species can be detected in the oral flora of edentulous infants. however, knowledge of the initial infection source is still scarce. thirty caucasian mothers (mean age 30 years) and their edentulous infants (mean age 3 months) were examined for the possible similarity of the oral gram-negative anaerobic flora. paraffin-stimulated saliva was collected from the mothers. a pooled swab sample from mucosal surfaces and unstimulated saliva were collected from ...19921494450
coaggregation studies of the eubacterium species.eubacterium species are gram-positive anaerobic rods that are frequently isolated from subgingival plaque of periodontal pockets. five eubacterium species were tested for their ability to coaggregate with 33 oral bacterial strains. using visual and turbidimetric assays, coaggregation was observed among eubacterium brachy, eubacterium nodatum, eubacterium alactolyticum and eubacterium limosum strains only when tested with fusobacterium nucleatum strains; eubacterium saburreum displayed only weak ...19921494452
characterization of shared antigens of fusobacterium nucleatum and fusobacterium necrophorum.fusobacterium nucleatum and fusobacterium necrophorum are gram-negative, non-spore-forming anaerobic rods, frequently isolated from the normal flora and diseased lesions of the human oral cavity, gastrointestinal and genitourinary tracts. f. necrophorum is also known to be an animal pathogen. studies were undertaken with rabbit anti-f. nucleatum sera and with human adult periodontitis (ap) sera that demonstrated the sharing of antigens between the two species. immunodiffusion and immunoelectroph ...19921494453
the breakdown and utilization of peptides by strains of fusobacterium nucleatum.it has been claimed that most strains of fusobacterium nucleatum require peptides rather than free amino acids for growth. in contrast, we have shown that, under continuous culture conditions, all strains tested grow in a chemically defined medium (cdm). the purposes of this study were to determine whether resting cells of f. nucleatum could attack unsubstituted peptides and whether growing cells could utilize a peptide fraction prepared from a commercial peptone. resting cells cleaved all 19 pe ...19921494454
golden hamster embryonic genome activation occurs at the two-cell stage: correlation with major developmental changes.the earliest time of onset of embryonic genome activation in golden hamsters was investigated. the inhibition of transcription by alpha-amanitin (11 micrograms/ml) in cultured embryos resulted in a total arrest of development of early 2-cell embryos (26 hr post-egg activation); under similar conditions, immediate cleavage divisions of 1-, late 2-, 4-, and 8-cell embryos were not affected. electrophoretic analysis of [35s]methionine-labeled embryonic proteins showed that alpha-amanitin treatment ...19921497872
primary culture of human fallopian tube epithelial cells and co-culture of early mouse pre-embryos.we have established a monolayer culture system for human fallopian tube epithelial cells. the cells were isolated from tubes using collagenase digestion, and were cultured in ham's f-10 supplemented with 15% fetal calf serum. the epithelial cells derived from culture were characterized using immunocytochemical staining and electron microscopy. these cells were stained with antikeratin and anti-epithelial membrane antigen, but showed no staining after treatment with antivimentin. electron microsc ...19921497873
[botryomycosis caused by fusobacteria].in the anglo-american literature botrymycosis is described as a chronic cutaneous granulomatous reaction to bacterial infection, containing granules resembling the sulphur granules seen in actinomycosis. the diagnostic and therapeutic aspects are discussed with reference to a recently observed patient. fusobacterium nucleatum was isolated as the bacterial cause of the pathologic disorder.19921506205
ruptured splenic abscess secondary to infectious mononucleosis.a 24-year-old woman with a recent diagnosis of infectious mononucleosis presented with fever and left upper quadrant pain. abdominal computed tomography revealed a splenic abscess that at laparotomy was found to have ruptured. this case illustrates a rare cause of splenic abscess and a rare complication of infectious mononucleosis.19921514735
the effect of cyclosporin and lipopolysaccharide on fibroblasts: implications for cyclosporin-induced gingival overgrowth.drug-induced gingival overgrowth is an adverse side effect associated principally with 3 different types of drugs; specifically the antiepileptic phenytoin, the calcium channel antagonist nifedipine, and the immunosuppressant cyclosporin. the present study has analyzed the effect of cyclosporin and lipopolysaccharide on fibroblasts from 3 different sources: 1) normal healthy human gingiva (nhgf); 2) overgrown gingiva from 2 patients taking cyclosporin (chgf); and 3) human fetal lung (wi-38). fib ...19921527683
the early colonization of gram-negative anaerobic bacteria in edentulous infants.the occurrence of oral gram-negative anaerobes was examined in 30 edentulous infants (mean age 3 months, range 1-7 months). one pooled swab sample from mucosal surfaces (cheeks, palate, tongue) and one saliva sample was taken from each infant. the samples were cultured aerobically and anaerobically using non-selective and selective media. prevotella (bacteroides) melaninogenica was the most frequently isolated anaerobe, found in 70% of the infants. the other common anaerobes were fusobacterium n ...19921528621
serum igg reactive with oral anaerobic microorganisms associated with infections of endodontic origin.numerous species of bacteria have been implicated with infections of endodontic origin. the purpose of this study was to compare the levels of serum igg antibodies reactive with a panel of 10 oral anaerobic microorganisms implicated in infections of endodontic origin. serum samples were collected from 4 patient groups that included healthy patients without endodontic or periodontal disease, patients with chronic adult periodontal disease, patients with endodontic disease and patients with combin ...19921528630
comparative mitogenicity and polyclonal b cell activation capacity of eight oral or nonoral bacterial lipopolysaccharides in cultures of spleen cells from athymic (nu/nu-balb/c) and thymic (balb/c) mice.optimal stimulatory doses of purified phenol-water extracted lipopolysaccharides (lps) from 5 selected strains of 3 putative periodontopathogens (fusobacterium nucleatum, prevotella intermedia, and veillonella), 3 strains of 2 nonoral bacterial species (bacteroides fragilis and salmonella enteritidis), and pokeweed mitogen (pwm) induced significantly higher maximum mitogenic responses and polyclonal ig production in cultures of unfractionated spleen cells from nu/nu-balb/c (nude) than from balb/ ...19921528635
a non-lectin-like mechanism by which fusobacterium nucleatum 10953 adheres to and activates human lymphocytes.most (approximately 80%) strains of fusobacterium nucleatum adhere to human erythrocytes in a lectin-like manner that is strongly inhibited by n-acetyl-d-galactosamine (galnac). in this study, we investigated the capacity of f. nucleatum 10953, a strain that is weakly inhibited by galnac, to adhere to and activate human lymphocytes in vitro. experiments using [3h]-labeled bacteria and scanning electron microscopy clearly showed that 10953 adhered to lymphocytes and that adherence was blocked by ...19921528636
isolation and properties of the capnocytophaga ochracea bacteriocin.a bacteriocin from a subgingival plaque isolate of capnocytophaga ochracea was purified and characterized. it was isolated from cell extract and had a molecular weight of 100,000. streptococcus sanguis, streptococcus mitis, streptococcus mutans and propionibacterium acnes were susceptible to the bacteriocin.19921528638
polymorphism in the rd (d6s45) gene.the rd (d6s45) gene in the class iii region of the hla major histocompatibility complex encodes a protein normally containing 24 consecutive basic-acidic dipeptide repeats. we determined the frequency of variations in the number of repeats by use of the polymerase chain reaction. of 107 subjects 7 (3.3%) carried genes encoding 22 or 23 repeats. there was no difference in the frequency of such polymorphisms between normal individuals and those with systemic lupus erythematosus, a disease associat ...19921534063
superoxide dismutase and catalase in an experimental model of multiple organ failure.multiple organ failure (mof) can be induced by sterile intraperitoneal injection of zymosan in the rat. this results in a typical triphasic illness with maximal clinical signs at days 2 and 12. in this model, superoxide production and lipid peroxidation closely correlate with the triphasic clinical illness. in the present experiment we studied the effect of albumin conjugated superoxide dismutase (sod) and catalase (cat) on lipid peroxidation and organ damage in the acute phase (days 1 and 2). l ...19921538604
regulations for implementing the clinical laboratory improvement amendments of 1988: a summary.in 1988, congress passed the clinical laboratory improvement amendments (clia), which set standards to improve the quality of clinical laboratory testing in all laboratories in the nation that conduct testing on human specimens for health assessment or for the diagnosis, prevention, or treatment of disease. this report summarizes the final regulations for implementing clia and provides an overview of each section, specifying laboratory standards and requirements.19921538689
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