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[immunobiological activity of 41k protein (porin) derived from the cell envelope of fusobacterium nucleatum]. 19863025315
ro 15-1788 suppresses the development of kindling through the benzodiazepine receptor.beta-carboline (norharman) has been shown to produce kindled seizures when given systemically for long periods of time. the expression of the kindled seizure activity can be blocked by ligands of the benzodiazepine receptor suggesting the receptor as a site of vulnerability in the kindling mechanism. the present data show that ro 15-1788, a receptor antagonist, suppresses the development of kindled seizures as demonstrated by the delayed appearance of each behavioral stage and the decreased seve ...19863026559
primary biliary cirrhosis: assessment of the quantitative importance of the adenine nucleotide translocator protein as a major mitochondrial antigen.the adenine nucleotide translocator protein (ant) is the first well-characterized mitochondrial polypeptide to be identified as an antigen for antimitochondrial autoantibodies (ama) in pbc sera. because of the potential use for a highly purified antigen as a tool in studying the aetiology of pbc, we have undertaken an assessment of the quantitative importance of ant as a pbc-specific mitochondrial antigen. immunoblotting and elisa techniques were used. both methods reveal pbc antibodies against ...19863028680
primary biliary cirrhosis: assessment of the quantitative importance of the adenine nucleotide translocator protein as a major mitochondrial antigen.the adenine nucleotide translocator protein (ant) is the first well-characterized mitochondrial polypeptide to be identified as an antigen for antimitochondrial autoantibodies (ama) in pbc sera. because of the potential use for a highly purified antigen as a tool in studying the aetiology of pbc, we have undertaken an assessment of the quantitative importance of ant as a pbc-specific mitochondrial antigen. immunoblotting and elisa techniques were used. both methods reveal pbc antibodies against ...19863028680
influence of n6-methylation of residue a(5) on the conformational behaviour of d(c-c-g-a-a-t-t-c-g-g) in solution studied by 1h-nmr spectroscopy. 1. the duplex form.one- and two-dimensional nmr studies at 300 mhz and 500 mhz were carried out on the two oligonucleotides d(c-c-g-a-a-t-t-c-g-g) and d(c-c-g-a-m6a-t-t-c-g-g) in aqueous solution. nmr spectra were observed at 10 mm sample concentration over the temperature range 273-368 k. assignments are given of the base, h1', h2', h2", h3' and of some h4' resonances, based upon a combination of two-dimensional correlation spectra (cosy) and two-dimensional nuclear overhauser effect spectra (noesy); imino-proton ...20103028800
influence of n6-methylation of residue a(5) on the conformational behaviour of d(c-c-g-a-a-t-t-c-g-g) in solution studied by 1h-nmr spectroscopy. 1. the duplex form.one- and two-dimensional nmr studies at 300 mhz and 500 mhz were carried out on the two oligonucleotides d(c-c-g-a-a-t-t-c-g-g) and d(c-c-g-a-m6a-t-t-c-g-g) in aqueous solution. nmr spectra were observed at 10 mm sample concentration over the temperature range 273-368 k. assignments are given of the base, h1', h2', h2", h3' and of some h4' resonances, based upon a combination of two-dimensional correlation spectra (cosy) and two-dimensional nuclear overhauser effect spectra (noesy); imino-proton ...20103028800
identification of a novel herpes simplex virus type 1-induced glycoprotein which complexes with ge and binds immunoglobulin.we detected a glycoprotein on the surface of cells infected with herpes simplex virus type 1 (hsv-1) which, in conjunction with ge, binds immunoglobulin g (igg). the novel glycoprotein, which has an apparent molecular mass of 70 kilodaltons and was provisionally named g70, was first detected in extracts of hsv-1-infected cells labeled by lactoperoxidase-catalyzed iodination and precipitated with rabbit sera or igg and protein a-sepharose. in subsequent experiments, g70 and ge were coprecipitated ...19873035221
herpes simplex virus immediate-early promoters are responsive to virus and cell trans-acting factors.the promoters for each of the immediate-early genes from herpes simplex virus type 1 were cloned and fused to a chloramphenicol acetyltransferase cassette. these chimeric genes were used as targets in a transient expression assay to determine how the immediate-early gene products icp4 and icp0 and the virion-associated stimulatory protein vmw65 affected their expression in hela and vero cells. the basal level of expression from these cassettes differed significantly depending on the extent of 5' ...19873035226
sv40-based escherichia coli shuttle vectors infectious for monkey cells.we describe sv40-based escherichia coli shuttle vectors which can be packaged as pseudovirions without excision of plasmid sequences and which can be rescued in bacteria. these vectors replicate and are transmitted as virus in monkey cos cells without requiring a helper virus. extrachromosomal vector dna isolated from infected cells can be rescued in e. coli, so that dna alterations can be easily screened. indeed, some of the constructions give rise to very stable plasmids with no detectable rea ...19873036664
source of iron in neutrophil-mediated killing of endothelial cells.recently we have shown that human neutrophils activated with phorbol ester are cytotoxic for cultured bovine pulmonary artery endothelial cells in an iron-dependent manner. by using the ferric iron chelator deferoxamine mesylate, we have now investigated the source of the iron. pretreatment of neutrophils with deferoxamine mesylate affected neither their production of o2- nor their cytotoxicity for endothelial cells after addition of phorbol ester. however, similar pretreatment of endothelial ce ...19873037192
production of immunoreactive growth hormone by mononuclear leukocytes.in the present study, we evaluated whether mononuclear leukocytes could synthesize and secrete growth hormone (gh) in vitro. by using rna slot blot analysis, we detected maximum spontaneous levels of specific gh mrna in the cytoplasm of rat leukocytes after a 4-h incubation. northern gel analysis demonstrated that the specific leukocyte gh rna was polyadenylated and had a molecular mass of 1.0 kb. further studies using immunofluorescence, antibody affinity chromatography, and sephacryl gel filtr ...19883044906
[skeletal changes following long-term treatment with retinoids].the synthetic retinoids, the vitamin-d-derivatives etretinate and isotretinoin, have substantially enlarged the therapeutic arsenal in dermatology. they are primarily used in severe cases of acne and cornification disorders. in the majority of cases, long-term treatment is necessary. certain side effects in the skeletal system can occur, e.g., osteoporosis, premature epiphyseal closure, and changes similar to dish (diffuse idiopathic skeletal hyperostosis). we discuss the reports in the literatu ...20113045876
[mass-spectrometric determination of specificity of proteinases].analysis of methods of the proteinase substrate specificity determination revealed that michaelis constants found from the rates of hydrolysis of small synthetic substrates cannot be a good measure of selectivity, especially for proteinases of broad specificity used for hydrolysis of substrates of high molecular mass with multiple cleavage sites. mass-spectrometric studies (eriad) of the hydrolysis of the insulin b chain with trypsin, chymotrypsin, and proteinase i showed that monitoring of the ...19883056418
intrapreneurship: tapping employee creativity.seeking ways to produce new products, processes, and programs that would result in cost savings or increased revenue for nursing services and the institution, as well as attracting and retaining bright, creative nursing employees, the authors developed a pilot program to allow intrapreneurs to "run with" their ideas. the authors discuss the mechanics of their program development, implementation, and evaluation as well as examples of innovative projects under development by nursing employees.20093057132
biomarkers of aging: immunological events.certain immune functions decline in a gradual way over the life span. to the extent that these changes are well characterized and are phylogenetically observed, they may be considered excellent biomarkers of aging. many of these age-related changes, however, are significantly influenced by age-associated phenomena such as certain diseases or psychosocial factors. immunologic factors, therefore, should be considered as markers of physiologic aging only under very careful experimental conditions.20113058494
ultrasound in chest disease: 2. lung and mediastinum. 20103060081
ultrasound in chest disease: 2. lung and mediastinum. 20103060081
[gustav adolf wehrli: a passionate collector. on the 100th anniversary of the founder of the museum of medical history in zürich]. 20103065885
identity--lost and found: patterns of migration and psychological and psychosocial adjustment of migrants.this paper focuses on patterns of integration in migrant individuals and families. the level of adjustment in traditional and westernized families has been examined. different factors affecting adjustment are pointed out. migration process is interpreted as a transient and necessary crisis situation. risk factors in the acculturation crisis are reported and predictive and preventive measures are suggested. characteristics of migrant and remigrant groups are compared. the role of "homesickness" i ...20113067526
behavioral pharmacology of stress: focus on cns corticotropin-releasing factor. 20113067556
[cryopreservation of early human embryo stages].a short review of freezing procedures applied to early human embryos is given. it is noted that human embryos survived freezing and thawing at a developmental stage of 1. cell to blastocyst. but it seems to be necessary to use for any developmental stage of early embryo a special freezing and thawing method. embryo survival is correlated with their morphologic features where as neither age of embryos nor developmental stage were involved in freezing and thawing ability.19883071049
regional localization of cd18, the beta-subunit of the cell surface adhesion molecule lfa-1, on human chromosome 21 by in situ hybridization.the gene coding for the beta-subunit of the cell surface adhesion glycoprotein lfa-1 has been localized to the tip of the long arm of chromosome 21 at 21q22.1-qter.19883073708
localization of the kras2 oncogene in the domestic rabbit (oryctolagus cuniculus).the kras2 gene was localized in the rabbit by chromosomal in situ hybridization, using a 3h-labeled human cdna probe. there were 201 silver grains on 144 metaphase spreads; 12.9% of the grains resided on chromosome 16, and 54% of these grains were located close to the centromere at 16p11----q11. statistical analysis indicated that labeling at this region represents a significant deviation from a random distribution and thus provided evidence for the assignment of kras2 to 16p11----q11. in additi ...19883073912
anaerobic infections in the head and neck region.anaerobic bacteria form the predominant flora of the oral cavity, outnumbering facultative organisms by 10-1,000: 1. the type of anaerobic bacteria and their concentration depend on the anatomical site and the degree of anaerobiosis in the different sites in the mouth. three groups of anaerobic bacteria inhabit the oral cavity; the strict anaerobes, the moderate anaerobes, and the microaerophilic group of organisms. the majority of anaerobic bacterial infections occurring in the region of the mo ...19883074469
reactive oxygen metabolites and hyperoxic toxicity. 20093074787
burkitt's lymphoma. 20103075349
burkitt's lymphoma. 20103075349
hmg coa reductase inhibitors. current clinical experience.lovastatin and simvastatin are the 2 best-known members of the class of hypolipidaemic agents known as hmg coa reductase inhibitors. clinical experience with lovastatin includes over 5000 patients, 700 of whom have been treated for 2 years or more, and experience with simvastatin includes over 3500 patients, of whom 350 have been treated for 18 months or more. lovastatin has been marketed in the united states for over 6 months. both agents show substantial clinical efficacy, with reductions in t ...20113076126
motor mechanisms in the turtle spinal cord.we reveal the intrinsic motor capacity of the spinal cord by examining motor behaviours produced by spinal segments caudal to a complete transection of the spinal cord. the turtle spinal cord generates three forms of the scratch reflex in the absence of neural inputs from supraspinal structures. each form exhibits a characteristic motor neuron discharge pattern. we test the ability of the spinal cord to generate organized motor patterns in the absence of movement-related sensory feedback by exam ...20093077002
reticulospinal neurons, locomotor control and the development of tailswimming in xenopus.the development of early swimming in xenopus occurs early during the embryonic period and within a few hours. between stages 25 and 33 the central nervous system reaches a state of 'critical mass' at which the for swimming necessary body structures have partly developed, thus enabling the embryo to move through the water. the pattern of undulatory body movements is formed within the pattern generators in the central nervous system (cns) involving different types of neurons in the spinal cord and ...20093077003
specificity and retinotectal projections of quarter-eye fragments in xenopus laevis.three quarters of the eye anlage in xenopus embryos of stage 33/34 were eliminated in three different sets of experiments. the remaining quadrant originated from the nasoventral part of the retina, from its ventral portion, or from the temporo-ventral area of the retina. all the fragments developed into small eyes of normal shape. the retinotectal connections did not deviate from those found in the control groups, even though mirror-image duplication was fairly frequent. for all fragments the te ...19883077005
processing of wave patterns in the lateral line system parallels to auditory processing.processing of wave patterns in the lateral line system is reviewed with particular reference to similarities with auditory processing. four levels are considered: the receptor cell, stimulus parameter encoding in the afferent nerve, the neural organization of stimulus localization, and analysis of complex waves. the high degree of parallelism at all these levels to auditory processing is considered as a strong evidence for a common evolutionary origin of these two systems.20093077007
early haemopoietic stem cells in the avian embryo.using 'yolk sac chimaeras', we have previously demonstrated that stem cells, destined to colonize haemopoietic organs other than the yolk sac, arise in the embryo proper. we have now investigated the emergence and potentialities of these cells in vivo and in vitro. the in vivo approach consisted of interspecies grafting between quail and chick embryos. the cell progeny from the grafts was detected by means of qh1, a monoclonal antibody specific for the quail haemangioblastic lineage. when grafte ...20113077941
1,25 dihydroxyvitamin d increases hepatocyte cytosolic calcium levels. a potential regulator of vitamin d-25-hydroxylase.1,25 dihydroxyvitamin d (1,25(oh)2d) has been demonstrated to inhibit hepatic 25 hydroxyvitamin d (25 ohd) production. changes in cytosolic calcium have been shown to regulate cellular processes. using the fluorescent dye quin 2, we have investigated the effects of 1,25(oh)2d and 24,25(oh)2d on cytosolic calcium levels in hepatocytes. 1,25(oh)2d exposure for 5 min increases cytosolic calcium levels by 24% at a concentration of 100 pg/ml, 39% at a concentration of 1 ng/ml, and 50% at a concentrat ...19863084563
ultrasonographic findings in the livers of patients with lepromatous leprosy.abdominal ultrasonography, including assessment of the liver, spleen, pancreas, great abdominal vessels, and kidneys, was carried out in seven patients with lepromatous leprosy, six patients with tuberculoid leprosy, and 32 healthy congolese controls. abnormal ultrasound findings were predominantly detected in the livers of patients with lepromatous leprosy and included an inhomogeneous echo texture of the hepatic parenchyma in all cases. furthermore, six patients revealed echo-dense, partly irr ...20103084566
diagnosis of leukemic relapse in the pelvic soft tissues of juvenile females.five females with childhood acute lymphocytic leukemia whose disease relapsed in the pelvis were studied. in all five patients, relapse occurred late in the disease process, and all patients had been in complete continuous remission before relapse. intravenous pyelogram and barium enema revealed hydronephrosis or a mass effect on the bladder or rectosigmoid colon. in three patients, sonography established the presence of a clinically palpable mass with involvement of the uterus, ovaries, and pel ...20103084568
nephrolithiasis and cholelithiasis in a premature infant. 20103084571
early second-trimester sonographic diagnosis of thoracopagus twins. 20103084572
prenatal ultrasonographic diagnosis of fetal adrenal neuroblastoma. 20103084577
chemiluminescence in the assessment of polymorphonuclear leukocyte function in chronic inflammatory periodontal disease.polymorphonuclear neutrophils (pmn's) constitute the primary host resistance factor against infection. they are prominent cells in both the gingival tissue and gingival sulcus in most forms of periodontal disease. although defective pmn function has been implicated in the pathogenesis of localized juvenile periodontitis (ljp) and rapidly progressive periodontitis (rpp), this may not necessarily be the case in adult periodontitis (ap). a number of studies have failed to detect pmn dysfunction in ...19863084735
some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate.lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ...19863085110
some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate.lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ...19863085110
[unwanted modification of the thyroid gland by drugs with special reference to nonsteroidal antirheumatic agents].in two retrospective clinical studies was investigated the influence of the modern non-steroidal antirheumatic drugs indometacin and diclofenac (rewodina) on the thyroid gland and corresponding peripheral hormone parameters. under longterm treatment with indometacin a moderate strumigenic effect could be observed, which could not clearly be proved under the diclofenac therapy. in all patients with rheumatoid arthritis, independent of the kind of pharmacotherapy, decreased t3-hormone levels were ...19863087075
associations between symptoms of irritable colon and psychological and social conditions and lifestyle.in a survey of risk factors for coronary heart disease 14 102 middle aged men and women answered a questionnaire on lifestyle, diet, and health, including symptoms of functional abdominal disorders. the overall prevalence of reports of one or both of the abdominal symptoms of "bloating and rumbling" or "cramping abdominal pain" was 28% in men and 35% in women. only a weak negative association between age and prevalence of reported pain was found in both sexes. women reported abdominal symptoms, ...20103087551
influence of glucocorticoids on calcium absorption in different segments of the rat intestine.glucocorticoids enhance the movement of fluid and sodium in the duodenum, thereby resulting in an increase in the passive transport of calcium. since passive transport of calcium predominates in the distal intestinal segments, the influence of glucocorticoids on calcium and fluid transport in the duodenum, mid-jejunum, ileum, and colon of the rat was studied. calcium transport and fluid absorption was determined by the in vivo ligated loop technique. one segment was ligated in each animal. under ...19863087601
antiimmunoglobulin-treated b cells respond to a b cell differentiation factor for igg1.we have determined whether b cells previously activated by anti-ig (anti-ig blasts) are responsive to lymphokines that induce isotype switching. culture of anti-ig blasts with a mixture of lymphokines, including bsf-1, resulted in marked secretion of igm and igg1, but not other igg isotypes. the igg1 response of anti-ig blasts to lymphokines was 13-fold greater than was observed with splenic b cells. b cell blasts induced by 8-mercaptoguanosine or dextran sulfate did not secrete high levels of a ...20113088197
hypophysectomy does not alter the increase in striatal dopamine receptor density produced by 6-hydroxydopamine lesions in male rats.while hypophysectomy can alter the increase in striatal dopamine (da) receptor density observed after estrogen, haloperidol or prolactin treatments, it does not alter the increase in density found after 6-hydroxydopamine lesions of the da neurons in male rats. this observation suggests that striatal da receptors can be regulated by at least two distinct mechanisms, only one of which requires the availability of pituitary hormones.19863092152
emergence of beta-lactamase producing anaerobic bacteria in the tonsils during penicillin treatment.the emergence of beta-lactamase producing bacteria in the microflora in the oropharyngeal cavity was studied in ten healthy volunteers treated with 1 g phenoxymethylpenicillin b.i.d. for ten days. beta-lactamase activity in saliva was also investigated. a significant increase in the number of beta-lactamase producing strains of bacteroides species and fusobacterium nucleatum was observed. one beta-lactamase producing staphylococcus aureus strain was recovered in one of the volunteers before the ...19863093222
[kinetic studies on indium-111-oxine labelled lymphocytes from patients with chronic lymphocytic leukemia]. 20113094311
on the mechanism of antithrombotic action of flavonoids.flavonols (quercetin and rutin) and flavanes (cyanidol and meciadonol) were studied for their effect on non-enzymatic lipid peroxidation, lipoxygenase and cyclo-oxygenase activities, binding to albumin and platelet membranes. these biochemical properties of four flavonoids were compared with respect to their antithrombotic action in vivo and their efficacy at influencing the platelet-endothelium interaction in vitro. all four flavonoids inhibited the ascorbate-stimulated formation of malondialde ...19873101704
hepatitis b, tropical ulcers, and immunisation strategy in kiribati.the seroepidemiology of hepatitis b was studied in kiribati (formerly the gilbert islands). six hundred and two (98%) of the population studied showed evidence of current or previous infection. the prevalence of hepatitis b surface antigen was 31% (188/615) and of the e antigen was 9% (58/615). infection was acquired early in life, and the prevalence of both antigens declined with age. the rates of infection were similar in all age groups examined (0-70) including early childhood. both hepatitis ...20103103763
age and infertility. 20113105771
age and infertility. 20113105771
comparison of the chinese schema and the international antigenic typing system for serotyping pseudomonas aeruginosa.twelve strains of pseudomonas aeruginosa representing 12 serogroups in the serogrouping schema used in the people's republic of china were compared with serogroups in the international antigenic typing system (iats). the first eight groups originated in the people's republic of china, and group ii appears to have a new major antigen that is not found in the iats. groups i, iii, iv, v, vi, vii, and viii correspond to groups 11, 6, 9, 4, 8, 3, and 1, respectively, of the iats. groups ix, x, xi, an ...19873108310
impact of troop dental health on combat readiness. 20083108716
the case for case management. 20103108737
cloning of reticulocyte lipoxygenase mrna. 19873109402
amino acid-dependent transport of sugars by fusobacterium nucleatum atcc 10953.resting cells of fusobacterium nucleatum 10953 (grown previously in a medium containing glucose) failed to accumulate glucose under aerobic or anaerobic conditions. however, the addition of glutamic acid, lysine, or histidine to anaerobic suspensions of cells caused the immediate and rapid accumulation of glucose. except for the amino acid-dependent transport of galactose and fructose (the latter being transported at approximately one-third the rate of glucose), no other sugars tested were accum ...19873114229
lysozyme-protease-inorganic monovalent anion lysis of oral bacterial strains in buffers and stimulated whole saliva.similar to streptococcus mutans, buffer suspensions of lactobacillus casei, lactobacillus plantarum, and fusobacterium nucleatum all undergo cell lysis when treated with the lysozyme-protease-inorganic monovalent anion antibacterial system. for lactobacillus species treated with lysozyme and proteases at phs of 4 and 5.3, lysis resulted when a lytic activating concentration of bicarbonate anion followed enzyme treatment. furthermore, synergistic lysis of these bacteria was noted when lysozyme-pr ...19873114343
[andrological abnormalities in internal diseases and following drug therapy].in the present paper the following non-endocrine internal diseases are discussed: liver cirrhosis, diabetes, chronic renal failure and morbus crohn. in alcoholic liver patients under fifty, hypospermia and oligozoospermia can be observed. the hormone assays showed moderately increased fsh- and lh-values in the serum; prolactin, testosterone and estradiol remained normal. an increased binding of testosterone to shbg is supposed, and the androgen deficiency symptoms are considered to be due to the ...20113115148
pathomorphological analysis of proliferative cystitis and its associated tumors. 20113115710
schistosoma mansoni polypeptides immunogenic in mice vaccinated with radiation-attenuated cercariae.we compared the humoral immune response of mice protected against schistosoma mansoni by vaccination with radiation-attenuated cercariae to that of patently infected mice, and we identified antigens that elicit a greater, or unique, immune response in the vaccinated mice. these comparisons were based upon radioimmunoprecipitations and immunodepletion of [35s]methionine-labeled schistosomular and adult worm polypeptides, followed by one- and two-dimensional polyacrylamide gel analyses. the humora ...19873116082
cerebral perfusion pressure: a hemodynamic mechanism of mannitol and the postmannitol hemogram.sixteen patients each received infusions of 1 g of mannitol per kg over 5 to 10 minutes, and serial determinations of intracranial pressure (icp), systemic arterial blood pressure (sabp), central venous pressure, cerebral perfusion pressure (cpp), hematocrit, hemoglobin, serum na+, k+, osmolarity, and fluid balance were carried out for 4 hours. urine output was replaced volume for volume with 5% dextrose in 0.45% nacl solution. we tested the hypothesis that patients with high (greater than or eq ...20103116451
the inhibition by valproic acid of the mitochondrial oxidation of monocarboxylic and omega-hydroxymonocarboxylic acids: possible implications for the metabolism of gamma-aminobutyric acid.the interactions of 1-5 mm valproic acid with the hepatic fatty acid oxidation are here described. valproic acid was not substrate for hepatic peroxisomal fatty acid oxidation. its activation outside the mitochondrial matrix compartment was poor when compared to that of octanoic acid, a fatty acid containing the same number of carbones. valproic acid did not inhibit the fatty acyl-coa oxidase nor the cyanide-insensitive acyl-coa oxidation. valproic acid inhibited the mitochondrial oxidations of ...19873117781
nutritional assessment with body composition measurements.the measurement of body composition by multiple isotope dilution provides an accurate and precise measure of both the nutritional state and the response to nutritional support. a multiple isotope dilution technique has been developed that permits measurement of the three major components of body composition: body fat, extracellular mass (ecm), and body cell mass (bcm). normal body composition was defined by data obtained in 25 healthy volunteers. malnutrition is characterized by a loss of bcm an ...20103118068
enprostil and ranitidine: comparative efficacy and safety in patients with duodenal ulcer.this randomised, double-blind, double-dummy, multiclinic study of duodenal ulcer healing compared the efficacy and safety of enprostil with ranitidine. the six week trial admitted 164 patients with endoscopically demonstrated duodenal ulcer. ratings of symptoms and adverse events were collated from patients' daily diaries, and endoscopy was repeated to verify healing after four weeks and, if appropriate, after six weeks. medication used was enprostil (35 micrograms capsule) or ranitidine hydroch ...20113118861
assessment of contractility changes in humans by transoesophageal echocardiography: the peak-systolic pressure end-systolic diameter relationship (pspesdrs).the peak-systolic pressure end-systolic diameter relationship (pspesdrs) was determined in ten healthy subjects under general anaesthesia by using the radial artery pressure tracing (peak-systolic pressure) and transoesophageal 2d-and m-mode echocardiography (left ventricular diameters) without blocking of autonomic reflexes. left ventricular load was changed by injection of a single intravenous dose of 0.2 mg nitroglycerin (ntg). linear regression analysis showed a close linear relationship dur ...20113119748
evaluation of the 11co2 positron emission tomographic method for measuring brain ph. i. ph changes measured in states of altered pco2.the 11co2 method for measuring local brain ph with positron emission tomography (pet) has been experimentally evaluated, testing the adequacy of the kinetic model and the ability of the method to measure changes in brain ph. plasma and tissue time/activity curves measured during and following continuous inhalation of 11co2 were fit with a kinetic model that includes effects of tissue ph, blood flow, and fixation of co2 into compounds other than dissolved gas and bicarbonate ions. for each of ten ...19873121647
deficiency of eosinophil peroxidase detected by automated cytochemistry. 20113122494
[fibrous lesions of the skeleton in infants and children]. 20113122625
lactate dehydrogenase and malate dehydrogenase of sertoli cells in rats.lactate-dehydrogenase (ldh) and malate-dehydrogenase (mdh) activities and isozyme patterns of maturing sertoli cells were investigated in cultured cells obtained from 2-5-week-old rats. both enzymes, and more strikingly ldh, increased between 2 and 3 weeks of age. ldh isozyme complement included all five isozymes and, in the more mature rats, had a shift toward the isoforms rich in subunit. these data are consistent with an increasing production of lactate/pyruvate and with a finer regulation of ...19873122678
[reduction of ventricular tachycardia by programmed electric stimulation. clinical implications].the mode of termination by programmed electrical stimulation of sustained ventricular tachycardia (vt) (more than 30 seconds) and induced by stimulation was investigated in 33 patients. in 27 patients (82%) programmed stimulation was possible because vt did not require an immediate electric shock or did not terminate spontaneously, but constant reduction of vt was obtained with one extrasystole in only 1 patient (3%), with two extrasystoles in 5 patients (15%) and by overdrive stimulation in 12 ...20113122686
identification of biological false positives in anti-hiv antibody tests. 19873122793
characterization of the ksga gene of escherichia coli determining kasugamycin sensitivity.in the plasmid puc8ksga7, the coding region of the ksga gene is preceded by the lac promoter (plac) and a small open reading frame (orf). this orf of 15 codons is composed of nucleotides derived from the lacz gene, a multiple cloning site and the ksga gene itself. the reading frame begins with the atg initiation codon of lacz and ends a few nucleotides beyond the atg start codon of ksga. the ksga gene is not preceded by a shine-dalgarno (sd) signal. cells transformed with puc8ksga7 produce activ ...19873122846
[effect of eicosanoids on the accumulation of cholesterol and the proliferation of subendothelial cells in the human aorta].the effect of prostacyclin and stable thromboxane analog a2 on endothelial culture of human aorta was studied. it was shown that prostacyclin inhibited accumulation of cholesterol in the cells and their proliferation, while thromboxane exhibited an opposite effect. calcium antagonists potentiated effects of prostacyclin and inhibited them in respect to thromboxane. screening of a number of synthetic agents affecting arachidonic acid metabolism was carried out. it was found that lipoxygenase inhi ...19883122858
cross-reactivity among legionella species and serogroups.cross-reactions of 17 members of the family legionellaceae were studied by four different serological techniques: immunofluorescence (if), slide agglutination (sa), microagglutination (ma) and immunodiffusion (id), using antigens and rabbit antisera prepared in our laboratory. results obtained corresponded closely with those described by other authors, especially for if and sa. the 17 antigens were further tested by if with a panel of sera previously diagnosed as positive for legionella. a high ...19873123264
field tests on four state-of-the-art blood chemistry analyzers in a mobile laboratory setting. 20113126442
recognition and prevention of pseudothrombocytopenia and concomitant pseudoleukocytosis.unrecognized anticoagulant-induced platelet (plt) aggregation, leading to pseudothrombocytopenia and concomitant pseudoleukocytosis, can have serious clinical consequences. it can be readily recognized by inspecting conventional blood smears or the white blood cell histograms generated by modern blood cell counters. blood specimens from twenty patients with known edta-induced platelet aggregation were consecutively drawn into three other anticoagulants (using vacutainer tubes) and processed in a ...20113128946
the national health service versus private and complementary medicine. 20113132291
the national health service versus private and complementary medicine. 20113132291
resting and exertional haemodynamic effects of buccal nitroglycerin: acute and chronic discontinuous treatment in post-myocardial infarction patients with heart failure.the resting and exertional haemodynamic effects of acute and chronic discontinuous (one tablet every 6 h) treatment with 5 mg of buccal nitroglycerin (bn) have been assessed in nine postinfarction heart failure patients. at rest, pulmonary artery (pap), pulmonary wedge (pwp), and right atrial pressures (rap) were reduced by 42%, 55% and 77%, respectively, after the first dose and by 26%, 32% and 45%, respectively, after the chronic (three weeks) treatment with bn. during exercise, at the same wo ...20113133210
effect of aldose reductase inhibition on nerve conduction velocity and resistance to ischemic conduction block in experimental diabetes.the relationships between nerve polyol levels and both nerve conduction velocity (ncv) and resistance to ischemic conduction block (ricb) in streptozocin-induced diabetic rats were examined in two studies. in the first study, sciatic ncv and ricb of the tail nerve, assessed by measuring the time to disappearance of the nerve action potential after the tail was rendered ischemic, were measured in nondiabetic rats, untreated diabetic rats, and diabetic rats given statil, an aldose reductase inhibi ...19883133261
synthetic aci-reductones: 3,4-dihydroxy-2h-1-benzopyran-2-ones and their cis- and trans-4a,5,6,7,8,8a-hexahydro diastereomers. antiaggregatory, antilipidemic, and redox properties compared to those of the 4-substituted 2-hydroxytetronic acids.synthetic procedures for the elaboration of aci-reductones belonging to the 6- or 7-mono- or bis-substituted-3,4-dihydroxy-2h-1-benzopyran-2-ones (6-10) and their cis- and trans-4a,5,6,7,8,8a-hexahydro diastereomers (11, 12) are described. hexahydrobenzopyranone aci-reductones were conveniently prepared by using meldrum's synthon (2,2-dimethyl-1,3-dioxane-4,6-dione, 49). certain of these substances were evaluated for antilipidemic activity in the cholesterol-fed rat model, and all analogues were ...19883133477
the effect of nicotine on human endothelial cell release of prostaglandins and ultrastructure.the acute and sub-acute effects of nicotine at concentrations between 10(-9)m and 10(-2)m on human umbilical vein endothelial cell release of prostacyclin and prostaglandin e2 and the sub-acute effect on the endothelial cell ultrastructure have been examined. the acute effect of nicotine on prostaglandin release has been assessed by measuring release of prostacyclin and prostaglandin e2 following stimulation of confluent monolayers of endothelial cells with a23187 in the presence of nicotine. th ...19883134041
influence of radiant heat stress on respiratory water loss in new-born lambs.respiratory water loss, oxygen consumption and carbon dioxide production were measured during rest and during moderate radiant heat stress in 9 healthy unanaesthetized lambs, age 4-15 days. the method used is based on an open flow-through system with a mass spectrometer for gas analyses. respiratory water loss was 12.7 mg/kg min when the lamb was at rest and increased to 24.7 mg/kg min during heat stress. oxygen consumption was 13.9 ml/kg min at rest and remained unchanged during heat stress. th ...19883134055
analysis of phorbol ester stimulated human megakaryocyte development.megakaryocytes are relatively rare components of human bone marrow, making the study of their maturation difficult. phorbol esters can act as differentiating agents in a number of cell systems including murine megakaryocytes. we report the effects of phorbol esters on the previously described long-term human megakaryocytic leukemia cell culture, est-iu. while two nontransforming phorbols fail to affect these cells, the transforming phorbol 12-o-tetradecanoylphorbol-13-acetate (tpa) induces a phe ...19883134065
analysis of phorbol ester stimulated human megakaryocyte development.megakaryocytes are relatively rare components of human bone marrow, making the study of their maturation difficult. phorbol esters can act as differentiating agents in a number of cell systems including murine megakaryocytes. we report the effects of phorbol esters on the previously described long-term human megakaryocytic leukemia cell culture, est-iu. while two nontransforming phorbols fail to affect these cells, the transforming phorbol 12-o-tetradecanoylphorbol-13-acetate (tpa) induces a phe ...19883134065
comparative studies on the low density lipoprotein subfractions from pig and man.(1) by means of a single spin density gradient ultracentrifugation method, sera from six human volunteers and six pigs were studied; three or four distinct low density lipoprotein (ldl) subfractions could be seen in the pig serum (ldl-1a, ldl-1, ldl-2 and ldl-3) and three in the human serum (ldl-1a, ldl-1b and ldl-2). the subdivision of ldl into subfractions was less clear for the human subjects than for the pigs. (2) physicochemical characteristics (chemical composition, apoprotein content, siz ...19883136968
value of cerebrospinal fluid analysis in the differential diagnosis of meningitis: a study in 710 patients with suspected central nervous system infection.a prospective study to determine the value of cerebrospinal fluid analysis in the differential diagnosis of meningitis was performed in 710 consecutively observed patients, both children and adults, who underwent lumbar puncture due to suspected central nervous system infection. diagnoses included acute or presumed bacterial meningitis (n = 79), acute or presumed viral meningoencephalitis (n = 218), acute unclassified meningitis (n = 6), other infections of the central nervous system (n = 37), n ...20113137038
[results of growth hormone treatment in slow-growing children with normal plasma growth hormone levels in provocation tests]. 19883137696
enzymatic capacity and protein synthesis in human muscle postoperatively.the glycolytic and aerobic oxidative capacity in skeletal muscle was investigated to reveal if the decrease seen in muscle protein synthesis is accompanied by a fall in the enzymatic capacity to oxidize substrates. six patients undergoing elective abdominal surgery were investigated by percutaneous muscle biopsies taken before surgery and on the first and third postoperative days. protein synthesis as assessed by the polyribosome concentration was 40% lower on the third day postoperatively than ...19883144105
specific responses to staphylococcus aureus in patients with hyper-ige syndrome. 19873144122
prognostic factors for nasopharyngeal carcinoma analysed by cox model. 20113148399
a new expression system for mammalian cells based on putative replicator sequences of the mouse and a truncated thymidine kinase gene.we have constructed a new expression vector for mammalian cells. the vector contains a truncated tk gene for amplification under selective conditions, a sequence putatively supporting the replication of plasmid dna in eukaryotic cells (murine autonomously replicating sequence) and an expression cassette for the cdna to be studied. as a model cdna we have used that of human tissue-type plasminogen activator (t-pa). analysis of hirt supernatants and chromosomal dna from l cells, prepared six weeks ...19883149608
an instructional module on testicular cancer for adolescent males. 20113152330
transplantation statistics in the uk--an agenda for the next quinquennium.our next quinquennial plan for transplantation studies extends mpi to corneal and unrelated marrow transplantation. it applies bayesian hierarchical modelling to regional variation in donor procurement and continues a program of special studies to augment national databases with respect to kidney, corneal, heart and liver transplantation. it promotes research collaboration among european and other organ exchange organizations as pioneered in the council of europe 1986 study on high sensitization ...20113154475
microbe-induced lymphocyte blastogenesis enhancement after preculture.the in vitro blastogenic response of human peripheral blood mononuclear cells to fusobacterium nucleatum and other oral microorganisms was enhanced if the peripheral blood mononuclear cells were cultured for 24 h at 37 degrees c prior to the addition of stimulant. the enhancement which occurred at optimal and supraoptimal concentrations of f. nucleatum (10 to 100 micrograms/ml) was detected after a preculture period of as little as 2 h. the blastogenic response was a result of t-cell proliferati ...19853156816
human and bovine endothelial cells synthesize membrane proteins similar to human platelet glycoproteins iib and iiia.human umbilical vein endothelial (huve) and bovine aortic endothelial (bae) cells in culture were examined to determine whether membrane proteins similar to human platelet glycoproteins (gp) iib and iiia were present. the huve and bae cells were either 125i-surface labeled or metabolically labeled. triton x-100 lysates of labeled cells were immunoprecipitated with polyclonal antibodies prepared against purified human platelet gp iib-iiia complex. two membrane proteins were detected on both huve ...19853161885
retreatodontics and ultrasonics. 20113162934
refractory periodontitis: mixed infection with bacteroides gingivalis and other unusual bacteroides species. a case report.host immune response and the predominant subgingival microflora were evaluated in a 47-year-old male exhibiting severe, recurrent periodontitis. the patient's neutrophils were chemotactically elevated but other functions were within normal limits. significantly, bacteroides gingivalis and bacteroides zoogleoformans constituted 80% of the cultivable microflora and total cell count in subgingival plaque. the remainder of the cultivable microbiota was comprised of fusobacterium nucleatum and haemop ...19883162983
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