TitleAbstractYear(sorted ascending)
regulation of fructose metabolism and polymer synthesis by fusobacterium nucleatum atcc for the anaerobic growth of fusobacterium nucleatum atcc 10953 can be derived from the fermentation of sugar (fructose) or amino acid (glutamate). during growth on fructose, the cells formed large intracellular granules which after extraction yielded glucose by either acid or enzymatic hydrolysis. the endogenous polymer was subsequently metabolized, and after overnight incubation of the cells in buffer, the glucan granules were no longer detectable by electron microscopy. anaerobically, w ...19902211506
cloning of wild-type pseudomonas solanacearum phca, a gene that when mutated alters expression of multiple traits that contribute to virulence.pseudomonas solanacearum undergoes a spontaneous mutation that pleiotropically reduces extracellular polysaccharide (eps) production, endoglucanase activity, and virulence and increases motility. we refer to the process that coordinately affects these traits as phenotype conversion (pc) and the resulting mutants as pc types. previous research with the wild-type strain aw1 suggested that inactivation of a single locus could mimic phenotype conversion (t. p. denny, f. w. makini, and s. m. brumbley ...19902211505
interaction of fusobacterium nucleatum 191 with human peripheral blood lymphocytes.fusobacterium nucleatum is frequently isolated in high numbers from the subgingival plaque of patients with periodontal diseases. adherence of these bacteria to several host tissues is lectin-like in that it is inhibited by galactose, lactose, and n-acetyl-galactosamine. since f. nucleatum has the capacity to activate human peripheral blood lymphocytes, the purpose of this study was to determine whether f. nucleatum adheres to lymphocytes and, if so, to determine whether lectin-like interactions ...19902148949
a rapid diagnosis of anaerobic infection in the oro-maxillary region by gas-liquid chromatography.the relationship between volatile fatty acids (vfas) in pus and infecting bacterial species was examined in order to establish a rapid identification system for anaerobic microorganisms in purulent inflammation in the oro-maxillary region. vfas were detected by the direct injection of pus into gas-liquid chromatography (glc). bacterial examination was carried out by anaerobic culture using blood agar plates. the bacterial identification was carried out mainly according to the vpi manual. analysi ...19902131168
serological investigation of refractory human adult periodontitis.this study examined the difference between the serum antibody profiles in refractory adult periodontitis patients (group a), and compared to those (group b) who responded well to conventional periodontal treatment. the levels of specific igg antibody to actinobacillus actinomycetemcomitans, bacteroides gingivalis, fusibacteriumnucleatum, and eikenella corrodens were assessed in a group of 19 patients (group a) and 11 patients (group b). specific igg serum antibody levels were estimated using bio ...19902096960
ingestion of bacteroides buccae, bacteroides oris, porphyromonas gingivalis, and fusobacterium nucleatum by human polymorphonuclear leukocytes in vitro.the phagocytic ingestion of clinical isolates and reference strains of bacteroides buccae, bacteroides oris, porphyromonas gingivalis and fusobacterium nucleatum by polymorphonuclear leukocytes (pmns) was studied. special attention was focused on the hydrophobicity of the strains. b. buccae strains, less or equally hydrophobic than pmns, were poorly ingested without opsonization. hydrophobic, but not hydrophilic, strains of b. oris and both hydrophilic and hydrophobic p. gingivalis and f. nuclea ...19902082243
distribution and frequency of fusobacterium nucleatum subspecies in the human oral cavity.three reference strains of fusobacterium nucleatum and 32 human oral isolates were compared by a variety of physiological tests, enzyme electrophoretic profiles, sds-page patterns, dna base composition and hybridization to test their possible site specificity and frequency of the recently described subspecies of f. nucleatum. nine of the 11 isolates assigned to f. nucleatum subspecies nucleatum were from diseased sites, whereas isolates from healthy sites were all identified as f. nucleatum subs ...19902098710
renin is sorted to the regulated secretory pathway in transfected pc12 cells by a mechanism which does not require expression of the pro-peptide.the rat pheochromocytoma cell line pc12 targets secretory proteins into two distinct pathways. when dna encoding human prorenin was transfected into pc12 cells, the protein was sorted into the regulated secretory pathway and released with similar kinetics to noradrenaline upon carbachol stimulation. to determine whether information for targeting prorenin lies within the pro-peptide we have transfected pc12 cells with a construct lacking the pro-peptide coding sequence. the transformed line secre ...19902194793
recolonization of the subgingival microflora after scaling and root planing in human periodontitis.the purpose of this study was to evaluate the recolonization patterns of the subgingival microflora of adult periodontitis patients after a single session of scaling and root planing. in each of eight patients, three clinically diseased sites were investigated microbiologically by darkfield microscopy and cultural analysis. after initial clinical and microbiological parameters were determined, each subject received a single session of scaling and root planing but no oral hygiene instructions. cl ...19902213468
[bcl-2 gene involved in t(14;18) translocation]. 19902255071
lysocardiolipin formation and reacylation in isolated rat liver mitochondria.liver mitochondrial cardiolipin (cl) is distinguished from other phospholipids by the presence of linoleoyl in almost all molecular species, and the biosynthesis of these species is not yet understood. the present study was carried out in order to test the hypothesis that the linoleoyl proportion of cl may be specifically enriched by a deacylation-reacylation cycle. incorporation of [14c]glycerol 3-phosphate into the metabolites of the cl pathway was accompanied by formation of 14c-labelled mono ...19902268287
coaggregation of oral candida isolates with bacteria from bone marrow transplant vitro coaggregation between candida species isolated from immunosuppressed bone marrow transplant recipients and oral bacteria was investigated. each candida strain showed a different pattern of coaggregation with the 22 bacterial strains studied. two strains of lactobacillus amylovorus isolated from separate bone marrow transplant patients and fusobacterium nucleatum (vpi 10197) coaggregated with all candida strains. ten bacterial strains showed no coaggregation with the candida strains. a v ...19902279992
associations between six dna probe-detected periodontal bacteria and alveolar bone loss and other clinical signs of periodontitis.the purpose of the present study was to assess the associations between the presence and amounts of actinobacillus actinomycetemcomitans, bacteroides gingivalis, b. intermedius, eikenella corrodens, wolinella recta, and fusobacterium nucleatum in the periodontal pocket and the degree of alveolar bone loss and other clinical signs of periodonitis, such as probing pocket depth, attachment level, and presence of bleeding on probing at the same site. the study material comprised 16 subjects with or ...19902288212
growth conditions and outer membrane proteins of fusobacterium nucleatum.fusobacterium nucleatum strains atcc 10953, atcc 25586, f1 f3, f6, and fev1 were grown in different media. the influence of growth conditions on the outer membrane proteins (omps) was analyzed by sodium dodecyl sulfate--polyacrylamide gel electrophoresis (sds-page). there were no apparent differences in the outer membrane protein profiles when cells in the same phase of growth in various rich media were compared. differences, however, were observed between early logarithmic phase and stationary ...19902349450
blastogenesis and polyclonal immunoglobulin synthesis in murine spleen cells stimulated with lipopolysaccharide, lipid a and acid-degraded polysaccharide from fusobacterium nucleatum.lipopolysaccharide of fusobacterium nucleatum strain fevl was split by acid hydrolysis. the split products, i.e. lipid a and degraded polysaccharide were mitogenic for murine spleen cells as measured by uptake of [3h]thymidine. the uptake of [3h]thymidine was dose-dependent. incubation of spleen cells with stimulants for 3 days resulted in a polyclonal activation of immunoglobulin synthesis. higher mitogenic response and immunoglobulin production were found in spleen cells of athymic mice compar ...19902369526
risk factors for kaposi's sarcoma in the vancouver lymphadenopathy-aids our ongoing cohort study of homosexual men, the ratio of new kaposi's sarcoma (ks) cases to new opportunistic infections (oi) during the periods 1982-1985, 1986-1987, and 1988-1989 fell from 0.75 (9 ks: 12 oi) to 0.57 (12 ks:21 oi) to 0.27 (4 ks:15 oi), respectively. to examine factors associated with the development of ks as compared to oi, we compared antecedent risk factors in 25 ks cases and 48 oi "controls." in univariate analyses, several classical hiv risk factors including numbers of ...19902395081
resistance to beta-lactam antibiotics in anaerobic bacteria.the known mechanisms of beta-lactam resistance in anaerobic bacteria involve production of beta-lactamases, alteration of penicillin-binding proteins, and blocked penetration of beta-lactam antibiotics through bacterial outer membranes. the most important factor in beta-lactam resistance is production of beta-lactamases, which have been found in various bacteroides, fusobacterium, and clostridium species. beta-lactam resistance in bacteroides fragilis is commonly mediated by beta-lactamases that ...19902406875
sialidase (neuraminidase) activity among gram-negative anaerobic and capnophilic bacteria.a filter paper spot test with 2'-(4-methylumbelliferyl)-alpha-d-n-acetylneuraminic acid as a substrate was used to study the prevalence of sialidase activity among gram-negative anaerobic and capnophilic bacteria. a total of 567 isolates representing four genera of obligate anaerobes and four genera of capnophilic organisms was tested. sialidase activity was detected in 94% of 66 isolates from the bacteroides fragilis group, 98% of 66 b. bivius isolates, and all isolates of the following species ...19902108991
serum antibodies to periodontal bacteria.the purpose of this study was to determine how serum antibodies reactive with periodontitis-associated bacteria with relates to the diagnosis of periodontitis subjects. study groups included localized juvenile periodontitis (ljp) subjects, severe periodontitis (sp) subjects, chronic adult periodontitis (ap) subjects, and age matched controls. twenty-two bacterial strains, representing 18 different species most commonly found in early onset periodontitis were evaluated using serum from ljp, sp, a ...19902117654
studies of the subgingival microflora in patients with acquired immunodeficiency syndrome.two unique forms of periodontal disease, hiv-gingivitis and hiv-periodontitis, have been described in patients with acquired immunodeficiency syndrome (aids). in order to determine the bacterial species associated with periodontitis in aids patients, the predominant cultivable microflora was examined in 21 subgingival plaque samples from 11 aids patients with periodontitis. the presence of putative periodontal pathogens including actinobacillus actinomycetemcomitans, bacteroides intermedius, por ...19902123926
monoclonal antibody-coated latex agglutination assay for identification of actinobacillus actinomycetemcomitans.three monoclonal antibodies (mabs) to lipopolysaccharide of actinobacillus actinomycetemcomitans strain y4 (serotype b) and eight mabs to a serotype b-specific polysaccharide antigen of strain y4 were obtained. latex particles sensitized with an mab to the y4 lipopolysaccharide produced a positive agglutination with whole cells of all three serotypes of a. actinomycetemcomitans, but not with haemophilus aphrophilus, haemophilus paraphrophilus, haemophilus influenzae, porphyromonas (bacteroides) ...19902124492
identification and characterization of human endothelial cell membrane binding sites for tissue plasminogen activator and urokinase.cultured human endothelial cells synthesize and secrete two types of plasminogen activator, tissue plasminogen activator (t-pa) and urokinase (u-pa). previous work from this laboratory (hajjar, k.a., hamel, n. m., harpel, p. c., and nachman, r. l. (1987) j. clin. invest. 80, 1712-1719) has demonstrated dose-dependent, saturable, and high affinity binding of t-pa to two sites associated with cultural endothelial cell monolayers. we now report that an isolated plasma membrane-enriched endothelial ...19902154465
characterization of the tet m determinants in urogenital and respiratory bacteria.tetracycline-resistant fusobacterium nucleatum, haemophilus ducreyi, mycoplasma hominis, peptostreptococcus spp., ureaplasma urealyticum, and veillonella parvula had dna sequences which showed homology throughout the length of the tet m transposon, tn916. in contrast, gardnerella vaginalis, commensal neisseria spp., and the 25.2-megadalton plasmid family lacked the complete transposon.19902159259
regulation of human monocyte/macrophage function by extracellular matrix. adherence of monocytes to collagen matrices enhances phagocytosis of opsonized bacteria by activation of complement receptors and enhancement of fc receptor inflammation monocytes emigrate from the peripheral circulation into an extravascular area rich in extracellular matrix proteins. in this milieu, phagocytes ingest and kill invading pathogens. in the present studies, we found that monocytes adhered to type i collagen gels phagocytized 2.5-12-fold more opsonized escherichia coli, staphylococcus aureus, streptococcus pyogenes, and streptococcus pneumoniae than plastic-adherent monocytes. the rate of phagocytosis and the number of bacteria inges ...19902168442
detection of bovine viral diarrhea virus in serum from cattle by dot blot hybridization assay.a dot blot hybridization assay was developed for use as a rapid screening test to detect bovine viral diarrhea virus (bvdv) in serum from infected cattle. a 1.1. kilobase cdna, prepared from the bvdv genome, was molecularly cloned and used in this study. insert cdna was removed from the puc9 plasmid vector by pst-i restriction endonuclease digestion and purified from plasmid dna by agarose electrophoresis and electroelution. the hybridization probe was prepared by nick translation in the presenc ...19902175527
transferable tet m in fusobacterium nucleatum.two tetracycline-resistant fusobacterium nucleatum strains were able to transfer, by conjugation, tetracycline resistance and the tet m determinant to recipient f. nucleatum, peptostreptococcus anaerobius, and enterococcus faecalis strains at a frequency of 10(-1) to 10(-8).19902178339
susceptibility of oral bacteria to phenoxyethanol and phenoxyethanol/chlorhexidine combinations.a total of 57 bacterial strains (26 different species) which may be isolated from subgingival plaque were tested for their in vitro susceptibility to phenoxycthanol, a commonly-used antiseptic and preservative. ninety-five percent of the strains, including those associated with chronic inflammatory periodontal disease, were susceptible to concentrations of phenoxyethanol used topically (2% w/v). phenoxyethanol at a concentration of 1% (w/v) was also found that to have a rapid bactericidal effect ...19902202809
the rna polymerases of porphyromonas gingivalis and fusobacterium nucleatum are unrelated to the rna polymerase of escherichia coli.western blot analysis that used antisera to the e. coli core enzyme and sigma factors was used for examination of the rna polymerase of actinobacillus actinomycetemcomitans, streptococcus mutans, fusobacterium nucleatum, and porphyromonas gingivalis. both antisera reacted with proteins in a. actinomycetemcomitans and s. mutans whole-cell extracts. reactions were seen with some f. nucleatum proteins when the anti-core rna polymerase antisera were used, but the cross-reacting proteins were not of ...19902204641
binding specificity of fusobacterium nucleatum to human erythrocytes, polymorphonuclear leukocytes, fibroblasts, and hela cells.eighteen strains of fusobacterium nucleatum were studied with respect to their adherence to human erythrocytes, polymorphonuclear leukocytes, fibroblasts, and hela cells. four types of adherence were found: 1) sugar-sensitive adherence 2) amino acid-sensitive adherence 3) unknown adherence 4) weak adherence. there was no obvious correlation between the patterns of hemagglutination and adherence to other cells. the results indicate not only a complex network of bacterial receptors on human cells, ...19902141873
plaque and chronic inflammatory periodontal disease. a question of ecology.the nature of the relationship between dental plaque and chronic inflammatory periodontal disease (cipd) remains unclear, although there is no doubt that plaque is the direct cause. non-specific, specific and exogenous hypotheses have been proposed to explain plaque-host relationships. current evidence indicates that plaque is part of the natural human microflora, one of many such in nature, and that disruption of oral microbial ecology, due primarily to diet texture changes, leads to gingivitis ...19902212083
glucose homeostasis in the rat after liver transplantation. 19902219327
radioimmunotargeting of human tumour cells in immunocompetent animals.a tumour model system is reported that for many purposes may be an alternative to xenografted nude mice. the model allows immunotargeting of human tumour cells in immunocompetent animals. the target cells are contained in i.p. diffusion chambers (dc) with micropore membrane walls that are permeable to molecules, including the cell specific monoclonal antibodies (moab), but impermeable to cells. thus, the tumour cells are protected from the host immunocompetent cells. in the work here presented t ...19902223574
radioimmunotargeting of human tumour cells in immunocompetent animals.a tumour model system is reported that for many purposes may be an alternative to xenografted nude mice. the model allows immunotargeting of human tumour cells in immunocompetent animals. the target cells are contained in i.p. diffusion chambers (dc) with micropore membrane walls that are permeable to molecules, including the cell specific monoclonal antibodies (moab), but impermeable to cells. thus, the tumour cells are protected from the host immunocompetent cells. in the work here presented t ...19902223574
[effect of enalapril on heart rate, arterial blood pressure and exocrine pancreatic secretion in the alert dog].a changed exocrine pancreatic secretion could be a pathogenetic factor of an acute pancreatitis after administration of angiotensin-converting enzyme (ace) inhibitors. in six conscious dogs with gastric and duodenal thomas fistulas we studied the effect of an intravenous (iv.) bolus injection of 10 mg enalaprilat, an intraduodenal (id.) bolus injection of 20 and 40 mg enalapril (e.), and 0.15 m nacl (20 ml iv., resp., id.) on pancreatic bicarbonate- and protein output in response to secretin (20 ...19902238764
the biotin-dependent sodium ion pump glutaconyl-coa decarboxylase from fusobacterium nucleatum (subsp. nucleatum). comparison with the glutaconyl-coa decarboxylases from gram-positive bacteria.membrane preparations of fusobacterium nucleatum grown on glutamate contain glutaconyl-coa decarboxylase at a high specific activity (13.8 nkat/mg protein). the enzyme was solubilized with 2% triton x-100 in 0.5 m nacl and purified 63-fold to a specific activity of 870 nkat/mg by affinity chromatography on monomeric avidin-sepharose. the activity of the decarboxylase was strictly dependent on na+ (km = 3 mm) and was stimulated up to 3-fold by phospholipids. the glutaconyl-coa decarboxylases from ...19902244788
capnophilic and anaerobic bacteremia in neutropenic patients: an oral source.the currently accepted empiric antibiotic therapy for bacterial infections in neutropenic patients may not cover the possibility of capnophilic and anaerobic bacteremia. many of these infections develop in patients with severe mucositis or periodontitis, and the type of organisms recovered also suggests an oral source of infection. we present two cases of bacteremia in neutropenic patients who had been empirically treated with ceftazidime and piperacillin plus amikacin. in the first case a beta- ...19902305182
species-specific oligodeoxynucleotide probes for the identification of periodontal bacteria.oligodeoxynucleotide probes were developed for identification of the periodontal bacteria actinobacillus actinomycetemcomitans, bacteroides gingivalis, b. intermedius types i and ii, b. forsythus, eikenella corrodens, fusobacterium nucleatum, haemophilus aphrophilus, streptococcus intermedius, and wolinella recta. probes were designed by sequencing the 16s rrna for each bacterium, identifying hypervariable regions, and chemically synthesizing species-specific probes. these probes were specific w ...19902312676
growth stimulation of treponema denticola by periodontal microorganisms.previous experiments have indicated that enrichment of subgingival plaque in human serum can lead to the accumulation of treponema denticola. t. denticola depends on bacterial interactions for its growth in serum. aim of the present study was to identify specific microorganisms involved in the growth stimulation of t. denticola. to this end, strains isolated from previous plaque enrichment cultures were tested for growth stimulation in co-cultures with t. denticola. in addition, growth of t. den ...19902321929
diisopropylfluorophosphate-binding proteins in the outer membrane of fusobacterium nucleatum: strain variations.six strains of fusobacterium nucleatum were tested for their ability to react with [3h]diisopropylfluorophosphate (dfp), a serine protease inhibitor. several cytoplasmic proteins were labelled but the strongest labelling was regularly observed in a few outer membrane proteins. the number and the molecular mass of the proteins detected varied according to the strain tested. a 61 kda protein was labelled in all strains tested, including the two type strains atcc 10953 and atcc 25586. a 65 kda prot ...19902323539
long-term clearance of inhaled uo2 particles from the pulmonary region of the rat.this paper reports on an enriched uranium dioxide (uo2) mass clearance study undertaken with fischer-344 rats. the uo2 had a uranium (u) isotopic composition of 0.79% 234u, 92.8% 235u, 0.34% 236u and 6.06% 238u, by mass, with an alpha-particle activity of 1.91 bq micrograms-1. forty-six rats were exposed to an enriched uo2 aerosol that had an activity median particle aerodynamic diameter ranging from 2.7 to 3.2 microns. the rats were killed from 1 to 720 d post-inhalation (pi). the mass of enric ...19902323929
susceptibility and resistance of plaque bacteria to minocycline.the in vitro susceptibility of 55 strains of subgingival plaque bacteria to minocycline was determined. a concentration of 1 microgram/ml minocycline was found to inhibit 85% of the strains tested and the mic ranged from 0.03 to 32 micrograms/ml. for 71% of the strains tested the mbc was at least 4 times greater than the corresponding mic, suggesting a bacteriostatic activity for minocycline. a concentration of 20 mg/ml of magnesium ions was capable of neutralizing 8 micrograms/ml of minocycline ...19902324922
genetic basis of tetracycline resistance in urogenital bacteria.the distributions of the nucleotide sequences related to the tetracycline resistance determinants tet k, tet l, tet m, and tet o were studied by dot blot hybridization with randomly chosen clinical urogenital tract isolates of viridans group streptococci, streptococcus agalactiae, enterococcus faecalis, gardnerella vaginalis, lactobacillus spp., fusobacterium nucleatum, peptostreptococcus spp., and veillonella parvula. among the peptostreptococcus spp., 79% of the isolates hybridized with one (6 ...19902327774
inhibitory effects of neuropeptide y on adenylate cyclase of rabbit ciliary processes.the inhibitory effect of neuropeptide y (npy) was studied on the adenylate cyclase (ac) activity in homogenates of rabbit ciliary processes and compared with that of the alpha 2-adrenergic agonist clonidine (cln). npy inhibited basal ac activity as well as ac activity stimulated by isoproterenol (iso), vasoactive intestinal polypeptide (vip) or forskolin (fsk). the extent of this inhibition corresponded well to the inhibition elicited by cln. the inhibitory effects of npy and cln appeared to be ...19902335109
amino acid utilization by fusobacterium nucleatum grown in a chemically defined medium.the present investigation evaluated amino acid utilization by 120 strains of fusobacterium nucleatum in a chemically defined medium and attempted to relate the patterns to 3 proposed subspecies of f. nucleatum. strains were inoculated into a chemically defined medium, with and without 2 g/l glucose, consisting of 14 inorganic salts, 21 amino acids, 23 vitamins and cofactors, and 7 purines and pyrimidines. after 7 days of anaerobic incubation, the spent culture medium, as well as the uninoculated ...19902080074
the incidence of periodontopathic microorganisms in young children.thirty-six children in 4 cohorts of 0-1 week, 1-6 months, 1-2 years and 2-2 1/2 years of age were examined for the presence of black-pigmented bacteroides species and some other bacteria suspected of being involved in the subsequent development of periodontal destruction. none of the sought-after bacteria were detected in the first week of life. bacteroides intermedius and bacteroides melaninogenicus were detected as early as 1 month after birth. both of these bacteria were detected in 16-37% of ...19902087345
role of cryptic receptors (cryptitopes) in bacterial adhesion to oral surfaces.progress in characterizing the receptors that promote bacterial attachment to teeth and oral epithelial cells has suggested that hidden molecular segments may frequently be involved. such cryptic receptors, referred to as 'cryptitopes', may become exposed by several mechanisms. hidden segments of salivary acidic proline-rich proteins evidently become exposed when the molecules undergo a conformational change as they adsorb to apatitic mineral. adhesins of actinomyces viscosus and certain other p ...19902088213
clinical and microbial evaluation of treatment regimens to reduce denture stomatitis.this study evaluated the effect of an antimicrobial mouthrinse, denture soft relines, and a placebo rinse on the clinical findings and microbial flora of 78 patients with denture stomatitis. for 28 days the study and control groups performed oral rinses and denture soaks. reline group patients had maxillary denture soft relines that were changed every 7 days. at the end of the study, the mean severity of denture stomatitis was significantly less in the groups that used the study rinse (p less th ...19902088373
[ecosystem of microbial flora in periodontal pockets, in vitro].this study was designed to analyze the succession of an ecosystem of microbial flora in periodontal pockets, in vitro. bacterial strains used in this study, were fusobacterium nucleatum atcc 10953, porphyromonas gingivalis 381, streptococcus sanguis atcc 10557 and streptococcus mutans omz 176. the bacterial cells, associated with beneficial, pathogenic or plaque forming activity, were cultured in brain heart infusion medium including hemin, vitamin-k1, l-cysteine hydrochloride and sodium thiogly ...19901966733
measurement of the human immune response to meningococcal lipooligosaccharide antigens by using serum to inhibit monoclonal antibody binding to purified lipooligosaccharide.we developed a human inhibition monoclonal enzyme-linked immunosorbent assay (himelisa) to investigate the human immune response to the lipooligosaccharides (los) of neisseria meningitidis. monoclonal antibodies (mab) were used to define seven epitopes on four los molecules of a meningococcal strain (126e) previously shown to express immunogenic los epitopes. the assay could distinguish epitope-specific antibody within whole sera. neither the specificity nor the amount of the antibody measured b ...19901694820
the relationship of serum igg antibody titers to periodontal pathogens to indicators of the host response in crevicular this study; the relationship of indicators of the local host response in gingival crevicular fluid (gcf) to the serum antibody titer to periodontal pathogens was examined. 15 patients with chronic adult periodontitis were studied. gcf was collected and analyzed for the total amount of igg, igm, the lysosomal enzyme b-glucuronidase (bg) and alpha-2-macroglobulin (alpha 2m). at the same examination, serum from these patients was collected, and enzyme-linked immunosorbent assays used to determin ...19901696949
permeability alteration of the sarcolemmal membrane, particularly at the site of macrophage contact, in experimental chronic trypanosoma cruzi myocarditis in mice.sarcolemmal membrane permeability characteristics have been investigated, particularly at the site of macrophage contact, in experimental chronic trypanosoma cruzi myocarditis in balb/c mice, employing ruthenium red (rr) as an electron tracer. the ultrastructural features of the myocardium from infected animals were similar to those previously described. briefly, focal myocarditis was detected, with areas of myocytolytic necrosis, atrophic myofibres, an inflammatory response composed of mononucl ...19901698094
[studies on defense effects of recombinant human granulocyte colony-stimulating factor (g-csf) to infections. ii. priming effect for superoxide production by human neutrophil].in present study, we have investigated superoxide (o2-) production from human neutrophils by recombinant human granulocyte colony-stimulating factor (g-csf) using the microtiter plate for the purpose of being close to the inflammatory site. g-csf by itself did not induce the release of o2- in human neutrophil on either fetal bovine serum (fbs)-coated plate or plate uncoated with fbs, even if neutrophils were exposed for maximum 3 hr. however, the optimal concentration of g-csf (50 ng/ml) was abl ...19901698209
conduction velocity distribution of afferent fibers innervating the rat urinary bladder.the conduction velocities of individual afferent fibers innervating the rat urinary bladder were determined by the antidromic stimulation of dorsal roots while recording from bladder postganglionic nerves. conduction velocities ranged from 0.5 to 21.0 m/s; 70% of the velocities were less than 2.5 m/s. the distribution within the dorsal roots was ipsilateral with 84% in l6 and 16% in s1. neuroanatomical tracing with horseradish peroxidase applied to individual bladder postganglionic nerves result ...19901698508
the influence of the bony pelvis in persistent occiput posterior position.roentgenologic pelvimetry has been performed in 68 consecutive cases of occiput posterior position. the results point to an influence from a transverse narrowing of the pelvis or a tendency towards large dimensions. in many cases, however, no clear correlation to pelvic dimensions is found. a significant difference exists in the rate of obstetrical complications between nulliparous and parous women. the reasons for this and for the persistent op position are discussed.1990269635
the false negative smear: an instrumental error? 1990269598
the fate of aflatoxin in naturally contaminated corn during the ethanol fermentation.corn naturally contaminated with aflatoxin was used as a substrate in the ethanol fermentation. distribution of toxin in several process and recovery fractions was identified. although little degradation of the mycotoxin occurred during fermentation, no toxin appeared in the distilled alcohol. as accumulation of toxin in spent grains represents a potential problem in use of the material as animal feed, several decontamination procedures were tested. sodium hydroxide, ammonium hydroxide, sodium h ...1990526887
abdominal ultrasonography in pediatrics. 1990171943
longitudinal sleep patterns during pubertal growth: four-year follow up.there were little or no indications of differences in sleep outcomes between the sexes. results indicate a disturbance of sleep on initial laboratory nights relative to later nights. the results reported here clearly document the persistence of these effects from year to year. for the most part, sleep characteristics during the 4 years immediately after onset of puberty appear to represent a typical phase in the gradual patterns of changes across all ages. total sleep time decreased markedly fro ...1990171619
increased risk of endometrial carcinoma among users of conjugated estrogens.the possibility that the use of conjugated estrogens increases the risk of endometrial carcinoma was investigated in patients and a twofold age-matched control series from the same population. conjugated estrogens (principally sodium estrone sulfate) use was recorded for 57 per cent of 94 patients with endometrial carcinoma, and for 15 per cent of controls. the corresponding point estimate of the (instantaneous) risk ratio was 7.6 with a one-sided 95 per cent lower confidence limit of 4.7. the r ...1990171569
acute brain trauma. 1991245244
isotopic and angiographic determination of cerebral blood flow. a correlation in patients with cerebral cerebral angiography has been performed on five patients who were demonstrated to have a cerebral circulatory deficit by the bedside isotope flow determination. in all five instances cerebral angiography confirmed the results of the isotope examination.1991207087
[the role of salivary immunoglobulins in dental caries]. 1991270178
subgingival microflora of advanced periodontitis in the dominican republic.a study of the predominant subgingival microflora was carried out in 24 periodontitis patients, 18 to 60 years of age, in santo domingo, dominican republic. paper point sampling, transport in vmga iii, and conventional microbiological techniques were utilized. direct microscopic examination revealed that cocci and nonmotile organisms made up 85% of the total organisms and spirochetes as little as 3%. nonselective culturing showed gram-negative organisms to constitute 53% of total isolates. fusob ...19911658290
relation of baseline microbial parameters to future periodontal attachment loss.the relationship between the level of subgingival species at baseline and subsequent attachment loss in a subject was examined. 38 subjects (14-71 years) with prior evidence of periodontal destruction were monitored 2x for pocket depth and attachment level at 6 sites per tooth at baseline and 2 months. a subject was considered to exhibit new attachment loss if 1 or more sites increased 3 mm or more in attachment level in 2 months. subgingival plaque samples were taken at the baseline visit from ...19911661304
systemic antibody responses to oral microorganisms in the cynomolgus monkey: development of methodology and longitudinal responses during ligature-induced disease.systemic antibody responses to oral microorganisms were studied during ligature-induced periodontal disease in a non-human primate (nhp) model. methodology was developed using elisa techniques to assess total igg and igm levels in the serum from the nhp. in addition, an elisa was developed utilizing affinity-purified anti-human isotype reagents to detect nhp serum antibody responses to porphyromonas gingivalis, actinobacillus actinomycetemcomitans, prevotella intermedia and fusobacterium nucleat ...19911665573
phenotypic analysis of b-cells extracted from human periodontal disease tissue.b-cells extracted from periodontal disease tissue were analyzed for the presence of activation markers using a range of monoclonal antibodies. in adult periodontitis (ap), 6% of b-cells expressed the il-2 receptor (cd25) compared with 1-2% in peripheral blood and healthy or marginal gingivitis (h/mg) gingival b-cells. there was also an increase in the mean percentage of igd-positive b-cells and a decrease in cd21 and cd22 expression. in both ap and h/mg lesions, 20-22% of the b-cells expressed c ...19911668249
osteomyelitis of the pelvis due to fusobacterium nucleatum.anaerobic pelvic osteomyelitis occurs rarely in children. a case of fusobacterium nucleatum osteomyelitis of the right ilium [correction of ileum] in a child without apparent risk factors is described. this case emphasizes the value of diagnostic aspirates. therapy with a combination of intravenous and oral clindamycin led to successful resolution of the infection.19911747843
the microflora of periodontal sites showing active destructive progression.20 adult periodontitis (ap) subjects were examined every 2 to 4 months and microbiological samples were collected and cultured when 2 mm or more loss of attachment (active sites) was detected by 2 examiners. similar sites in which no progressive destruction was observed (control sites) also were sampled in the same subjects. by lambda-analysis, there was no statistically significant difference in floras of active (42 sites from 12 subjects) and control (36 sites from 12 subjects) sites or betwee ...19911752997
analysis of high psa n-cam expression during mammalian spinal cord and peripheral nervous system development.using a monoclonal antibody that recognizes specifically a high polysialylated form of n-cam (high psa n-cam), the temporal and spatial expression of this molecule was studied in developing spinal cord and neural crest derivatives of mouse truncal region. temporal expression was analyzed on immunoblots of spinal cord and dorsal root ganglia (drgs) extracts microdissected at different developmental stages. analysis of the ratio of high psa n-cam to total n-cam indicated that sialylation and desia ...19911769342
differential protective activities of site specific lipoxygenase inhibitors in endotoxic shock and production of tumor necrosis factor.lipoxygenase inhibitors have been shown to exert beneficial effects in experimental models of endotoxin shock. in the present study it was found that lipoxygenase inhibitors prevented lps, but not tumor necrosis factor alpha (tnf alpha)-evoked leukopenia in mice. these inhibitors protected against endotoxin lethality but not against tnf alpha induced lethality. when the protective potency of the specific 5-lipoxygenase inhibitors (mk 886, cgs 81585) was tested in endotoxin-induced leukopenia and ...19911783470
[subgingival microflora in children of early childhood, school age and circumpuberty. the proportion and frequency of gram-negative bacteria in periodontally healthy and gingivitis groups].the present study characterized the microbial profiles of the gingival sulci in children. subgingival samples from 36 gingivitis lesions of 18 patients and 36 sites in 18 healthy persons were examined. the tested individuals were divided into three stages according to physiological maturation i.e. early childhood, school age, circumpuberty. puberty was confirmed through examination of wrist radiographs. using continuous anaerobic techniques, samples were dispersed, diluted and then inoculated on ...19911784868
comparative in vitro activity of cefpodoxime against anaerobes other than bacteroides assess the in vitro activity of cefpodoxime against anaerobic respiratory tract and oropharyngeal pathogens 77 strains belonging to 18 gram-negative and 7 gram-positive species were studied by means of agar dilution tests. for comparison cefuroxime, amoxicillin, amoxicillin + clavulanic acid and clindamycin were also tested. cefpodoxime was found to be active at concentrations of less than or equal to 0.125 mg/l against prevotella oralis, prevotella buccalis, prevotella bivia, porphyromonas a ...19911800380
bacteria in the apical 5 mm of infected root canals.ten freshly extracted teeth which had carious pulpal exposures and periapical lesions contiguous with the root apex were placed inside an anaerobic chamber and the apical 5 mm of the root canals cultured. in addition to anaerobic incubation, duplicate cultures were incubated aerobically. fifty strains of bacteria from the 10 root canals were isolated and identified. the most prominent bacteria cultured from the 10 root canals were actinomyces, lactobacillus, black-pigmented bacteroides, peptostr ...19911809801
[the dynamic modification of aseptic inflammation with sodium oxybutyrate].the oxybuturate sodium influence on the vessel and cells dynamics in the source of aseptic inflammation was studied. it was shown that oxybuturate sodium injection (100 mg/kg) before 30 min and after 2 and 4 hours of inflammation onset leads to suppression of microvessel reaction, reduction of macrophagic infiltrate density, decrease of macrophagic phase duration and the earliest perfection of inflammation in the whole.19911810478
a heat stable hemagglutinating activity of eikenella corrodens.strains of eikenella corrodens and fusobacterium nucleatum were shown to possess lectinlike attachment factors. the present investigators found that e. corrodens has a heat stable factor which agglutinates sheep, mouse, and human erythrocytes. the activity is not abolished by additions of galactose or lactose. lipopolysaccharide preparations from e. corrodens strains possessed strong hemagglutinating activity. lpss of f. nucleatum did not agglutinate any erythrocytes tested. sds-page patterns st ...19911819444
adhesive properties of strains of fusobacterium nucleatum of the subspecies nucleatum, vincentii and polymorphum.this study surveyed some adhesive properties of strains of fusobacterium nucleatum representative of the 3 recently defined groups or subspecies that could relate to their colonization and virulence. with one exception, f. nucleatum strains agglutinated sheep erythrocytes, but the quantity of bacteria required and the sensitivity of the hemagglutination reactions to inhibition by 0.05 m galactose or arginine varied between strains, and did not exhibit clear-cut correlations with subspecies. neur ...19911820561
comparison of the amino acid uptake profile of reference and clinical isolates of fusobacterium nucleatum subspecies.human isolates of fusobacterium nucleatum subspecies appear to colonize different niches in the oral cavity, which may be reflected in their nutritional properties. consequently the utilization of nitrogenous substrates, their sources of energy (supplied here as amino acids) were compared between the 3 subspecies using the reference strain and fresh clinical isolates of each subspecies. all strains incorporated mainly acidic and basic amino acids but significant differences occurred between subs ...19911820562
predominant cultivable flora in pericoronitis.the predominant cultivable flora in pericoronitis was investigated by culturing pus from affected sites in 20 patients. twenty colonies were picked at random from non-selective plates and identified using conventional biochemical and physiological tests, analysis of metabolic end-products by gas chromatography and protein profile analysis. the most frequently isolated organisms were prevotella (bacteroides) intermedia, peptostreptococcus micros, veillonella species, fusobacterium nucleatum and s ...19911820571
antimicrobial activity of flurbiprofen and ibuprofen in vitro against six common periodontal pathogens.nonsteroidal antiinflammatory drugs (nsaids) such as flurbiprofen and ibuprofen, have been shown to inhibit the inflammation and alveolar bone loss associated with chronic destructive periodontal disease. however, the direct effect of nsaids on the gingival crevice microflora has not been studied. the purpose of this investigation was to evaluate the antimicrobial activity of ibuprofen and flurbiprofen in vitro on six commonly isolated periodontal pathogens. the bacterial strains evaluated were ...19911812907
[effect of the type of swab on the isolation of chlamydia trachomatis].the success in the isolation of c. trachomatis is dependent on several factors. one of them is the type of swab used for collection the specimens. as some types of swabs, such as calcium alginate and aluminum, cotton and wood have recently been shown to decrease the isolation rate, different types of swabs were prepared and tested in our laboratory. commercial aluminum, type 5056 aluminum and stainless steel were used with the same type of cotton. commercial swabs of cotton and wood were tested ...19911815280
multicenter evaluation of tetracycline fiber therapy. iii. microbiological a multicenter study of the effects of tetracycline (tc) fiber therapy, subgingival plaque samples were tested for 6 probable periodontal pathogens by dna probe analysis. levels of actinobacillus actinomycetemcomitans, eikenella corrodens, fusobacterium nucleatum, porphyromonas (bacteroides) gingivalis, prevotella intermedia (bacteroides intermedius), and wolinella recta were quantitatively determined in samples taken at baseline, and immediately after tc fiber removal, control fiber removal, ...19911832457
tetracycline fiber therapy monitored by dna probe and cultural methods.oligonucleotide dna probe and selective cultural methods were compared in their ability to monitor 6 putative periodontal pathogens in a study evaluating local tetracycline fiber therapy. subgingival plaque was sampled from 4 sites in each of 20 subjects. samples were taken before and after therapy from sites assigned to the following test groups: tetracycline (tc) fiber, scaling and root planing, control fiber, and untreated. each sample was analyzed by both dna probe and cultural methods. tota ...19911832458
neurons of the pretectal area convey spinal input to the motor thalamus of the cat.the aim of this study was to corroborate lesioning work (mackel and noda 1989), suggesting the pretectal area of the rostral midbrain acts as a relay between the spinal cord and the ventrolateral (vl) nucleus of the thalamus. for this purpose, extracellular recordings were made from neurons in the pretectal area which were antidromically activated by stimulation in the rostral thalamus, particularly in vl. the neurons were tested for input from the dorsal columns of the spinal cord, the dorsal c ...19911855554
bacterial flora of the uterus of cows after calving on two hygienically contrasting farms.intrauterine swabs were obtained from cows after calving on two commercial dairy herds with contrasting hygienic environments and incidence of leucorrhea, and cultured aerobically and anaerobically. of 26 cows with a normal calving and puerperium, eight of 14 (57 per cent) were sterile on farm b where hygiene was poor, compared with five of 12 (42 per cent) on farm a where hygiene was better. two cows on farm b retained their placentas and subsequently developed metritis/endometritis. actinomyce ...19911858270
peptidoglycan precursor from fusobacterium nucleatum contains lanthionine.fusobacterium nucleatum was grown in the presence of [14c]udp. by means of sequential precipitation and chromatographic separation of the cytoplasmic content, a peptidoglycan [14c]udp pentapeptide containing lanthionine was isolated. this finding indicates that lanthionine is synthesized and incorporated as such during the assembly of the peptidoglycan.19911987169
microbiological and immunological aspects of experimental periodontal disease in rats: a review article.animal models in which microbiological and immunological aspects of periodontal disease can be studied prospectively seem well warranted. the rat bears much resemblance to man with respect to periodontal anatomy, development and composition of dental plaque, histopathology of periodontal lesions, and basic immunobiology. furthermore, reproducible methods are available for assessment of periodontal disease in rats, and detectable periodontal destruction can be induced in a few weeks in these anim ...19912002433
the helicobacter (campylobacter) pylori-colonized duodenal mucosa and gastric metaplasia.biopsies were obtained from non-ulcerated sites of the duodenum from 100 dyspeptic patients. helicobacter (campylobacter) pylori was cultivated from 19 of these biopsies. active chronic duodenitis (acd) was found in 17 biopsies and more than 5% gastric metaplasia in 20 biopsies. h. pylori as well as acd occurred with a significantly increased frequency when more than 5% gastric metaplasia was found in the duodenal biopsies. h. pylori on metaplastic tissue without acd was, however, seen in two ca ...19912018637
control of enterococcus faecalis sex pheromone cad1 elaboration: effects of culture aeration and pad1 plasmid-encoded determinants.aeration of plasmid-free enterococcus faecalis strains resulted in an 8- to 16-fold decrease in sex pheromone cad1 activity in culture filtrates. levels of two unrelated pheromones, cpd1 and cam373, were unaffected by culture aeration. aeration also resulted in a decrease in the expression of conjugative transfer functions observed in cells containing pad1 trab mutations, verifying a link between trab function and pheromone "shutdown." tests with a series of pad1 mini-plasmids indicated that the ...19911924555
tetracycline resistance in peptostreptococcus species.of 15 peptostreptococcus sp. strains isolated between 1975 and 1984, 13 hybridized with the tet k, tet m, or tet o determinant. a donor peptostreptococcus anaerobius strain carrying the three determinants could transfer tet m to p. anaerobius and fusobacterium nucleatum recipients but not to an enterococcus faecalis recipient, while neither tet k nor tet o was transferred.19911929341
purification and characterization of a 65-kilodalton diisopropylfluorophosphate-binding protein in the outer membrane of fusobacterium nucleatum fev1.a 65-kilodalton protein was identified in the outer membrane of fusobacterium nucleatum fev1 by sds-page. the relative amount of the protein varied during growth, being greatest in stationary phase. the protein was radio-labeled by [125i]-lactoperoxidase treatment of live cells and was only partially extractable with 2% sodium dodecylsulfate (sds) at room temperature, and therefore assumed to be both exposed to the cell surface and peptidoglycan associated. in intact cells the protein bound diis ...19912047749
[severe infections due to streptococcus milleri in children].streptococcus milleri (s. milleri) is found in healthy individuals in the mouth, nasopharynx, throat, vagina and in feces, and has been reported to be isolated from several infectious diseases in man, particularly from abscess in various parts of the body. we report two cases of severe infections due to s. milleri isolated from subdural abscess and pleural empyema. [case 1] 13 year old boy who had been healthy until he was noticed to have meningeal signs and was diagnosed as left subdural absces ...19911680938
intrageneric relationships of members of the genus fusobacterium as determined by reverse transcriptase sequencing of small-subunit rrna.the phylogenetic interrelationships of 14 members of the genus fusobacterium were investigated by performing a comparative analysis of the 16s rrna sequences of these organisms. the sequence data revealed considerable intrageneric heterogeneity. the four species fusobacterium nucleatum (including f. nucleatum subsp. nucleatum, f. nucleatum subsp. polymorphum, "f. nucleatum subsp. fusiforme," and "f. nucleatum subsp. animalis"), fusobacterium alocis, fusobacterium periodonticum, and fusobacterium ...19911715737
quantitative analysis of the epstein-barr virus-inducing properties of short-chain fatty acids present in the culture fluids of oral bacteria.the culture fluids of various anaerobic bacteria induced the synthesis of early antigens (ea) in epstein-barr virus (ebv) carrying lymphoblastoid cells. the culture fluids of corynebacterium butyricum and fusobacterium nucleatum were the effective inducer on ea. the inducing activity was, to some extent, dependent on their n-butyric acid content, but appeared to be regulated by yet unidentified materials.19911652240
synergistic effect on blastogenesis in murine spleen cells of lipopolysaccharide, lipid a, and acid-degraded polysaccharide from fusobacterium nucleatum.interchangeable combinations of fusobacterium nucleatum fev1 lipopolysaccharide (lps) with its split products by acetic acid hydrolysis, i.e. lipid a (la) and degraded polysaccharide (ps), amplified the blastogenic response in murine spleen cell cultures as measured by [3h]thymidine uptake. athymic murine spleen cells precultured with lps-fev1 for 48 h (stage 1), washed twice and cultured together with fresh cells and either la or ps for 72 h (stage 2) gave a synergistic response over that found ...19912059480
a study of the antigenic composition of the fragilis group of bacteroides.representative strains of 22 serotypes of the fragilis group of bacteroides and four non-fragilis control strains of b. melaninogenicus, b. disiens, b. bivius and fusobacterium nucleatum were tested by sds-page and immunoblotting with hyperimmune rabbit sera. sds-page showed 25 polypeptide bands but, after immunoblotting, 24 antigenic bands were observed in various combinations in all the strains. three of these were detected only in the control strains, whereas six others were present in differ ...19911875392
isolation, purification and characterisation of 2-oxoglutarate reductase from fusobacterium nucleatum.2-oxoglutarate reductase from fusobacterium nucleatum was isolated by thiol-disulphide interchange covalent chromatography. the enzyme was purified approximately 4000-fold and had a molecular mass of 68 kda. the michaelis constants for 2-oxoglutarate and nadh were 6.4 x 10(-5) and 0.4 x 10(-5), respectively. the involvement of sulphahydryl groups in catalysis was shown from the inhibition of 2-oxoglutarate reduction in the presence of 2,2'-dipyridyl disulphide and reactivation with 2-mercaptoeth ...19911884985
the use of genomic dna fingerprinting in studies of the epidemiology of bacteria in periodontitis.recent studies of microbial epidemiology emphasizing the genetic organization and distribution of organisms associated with orofacial infections have led to new insights into the possible origins of pathogenicity. studies into genetic heterogeneity, acquisition and transmission of these organisms have been markedly advanced by the utilization of the powerful technique of genomic dna fingerprinting. characteristic fingerprints for each bacterial isolate can be produced by cleavage of high molecul ...19911890219
dental bacterial plaques. nature and role in periodontal disease.antony van leeuwenhoek first described oral bacteria. however, not until almost 200 years later was the famous koch postulate introduced. since then, research has extensively been performed regarding the development and microbiology of dental plaques. in spite of the complexity of the developing flora of supragingival plaque, culture studies have shown a remarkably orderly succession of organisms. lately, the concept of microbial specificity in the etiology of periodontal diseases has been widel ...19911890226
patterns of plasma sex hormone-binding globulin, thyroxine and thyroxine-binding globulin in relation to reproductive state and hibernation in female little brown bats.a sex hormone-binding globulin (shbg), which bound both oestradiol and dihydrotestosterone, was studied in the plasma of adult female little brown bats throughout the annual reproductive cycle. this protein was present at low baseline levels from september to may inclusive, months which correspond to the periods of hibernation, ovulation and early pregnancy. during the second half of pregnancy in june, shbg levels increased 15- to 30-fold and remained increased throughout lactation and anoestrus ...19911900322
biological functions of the water-insoluble fraction of mouse seminal vesicle fluid. i. suppression of the blastogenic response of lymphocytes.the effects of the water-insoluble fraction of mouse seminal vesicle fluid (wif-svf) on lymphocytes was investigated to clarify its role in reproductive immunity. wif-svf inhibited the blastogenic response of t-cells to concanavalin-a (con-a), but it did not inhibit the blastogenic response of b-cells to lipopolysaccharide (lps). pretreatment of splenocytes with wif-svf did not suppress the blastogenic response of splenocytes to con-a when treated cells were washed prior to culture. wif-svf did ...19911906049
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