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molecular identification and quantification of bacteria from endodontic infections using real-time polymerase chain reaction.it was the aim of the present study to evaluate root canal samples for the presence and numbers of specific species as well as for total bacterial load in teeth with chronic apical periodontitis using quantitative real-time polymerase chain reaction (pcr).200818793361
rapid identification of oral anaerobic bacteria cultivated from subgingival biofilm by maldi-tof-ms.to facilitate the identification of anaerobes cultivated from periodontal disease, whole cell bacterial identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof-ms) was evaluated.200818793359
the role of toll-like receptors 2 and 4 on reactive oxygen species and nitric oxide production by macrophage cells stimulated with root canal pathogens.periapical lesions arise as a result of the activation and interaction of the host immune responses against root canal infection. recently identified toll-like receptors (tlr) seem to be involved in the recognition and development of immune responses against a myriad of microorganisms. however, very little information is available on the role of tlr in the induction of periapical lesions.200818793356
[bacteremia after periodontal procedures].bacteremia frequently occurs after oral surgery and odontology procedures. periodontitis may affect the incidence and bacterial spectrum of bacteremia. periodontal disease may be a significant risk factor for the development of certain systemic diseases. this study has aimed to evaluate the frequency of aerobic and anaerobic bacteria in the bloodstream following scaling and root planing.200818792815
[characterization and antibacterial effect of ag-nha-ntio2/polyamide 66 nanocomposite membrane on oral bacteria].undried silver-hydroxyapatite-titania (ag-nha-ntio2) nanoparticles slurry was used to make membrane with polyamide 66 (pa66) by co-polymerization method. the purpose of this study is to test the physical and chemical characteristics and antibacterial ability.200818780488
identification of a methioninase inhibitor, myrsinoic acid b, from myrsine seguinii lév., and its inhibitory activities.a methioninase inhibitor from myrsine seguinii was purified and identified as myrsinoic acid b. its inhibitory activities as to crude methioninase from periodontal bacteria such as fusobacterium nucleatum, porphyromonas gingivalis, and treponema denticola were determined. the ic50 values were 10.5, 82.4, and 30.3 microm respectively.200818776663
in vitro bactericidal activity of recombinant human beta-defensin-3 against pathogenic bacterial strains in human tooth root canal.human beta-defensin-3 (hbd3), an endogenous antimicrobial peptide, has strong broad-spectrum antimicrobial activity. this study aimed to obtain recombinant hbd3 (rhbd3) and to test the hypothesis that the antimicrobial characteristics of hbd3 may offer an advantage over conventional medicine in reducing intracanal bacteria. genetic engineering was used to obtain active rhbd3 and analysis revealed that it exhibited a broad spectrum of antibacterial activity at low micromolar concentrations agains ...200918775647
photodynamic therapy as an adjunct to non-surgical periodontal treatment: a randomized, controlled clinical trial.recent preclinical and clinical data have suggested a potential benefit of photodynamic therapy (pdt) in the treatment of periodontitis. however, there are very limited data from controlled clinical trials evaluating the effect of pdt in the treatment of periodontitis. the aim of this study was to evaluate the clinical and microbiologic effects of the adjunctive use of pdt to non-surgical periodontal treatment.200818771363
laboratory maintenance of fusobacteria.this unit describes routine laboratory handling of fusobacteria. different media that can be used to grow or enrich fusobacterium nucleatum and other species of this genus are described. the growth and stock conditions as well as the susceptibility of f. nucleatum to oxygen in a pure culture are also discussed.200618770577
rapid quantification of periodontitis-related bacteria using a novel modification of invader plus technologies.the invader plus technology is a sensitive, rapid method for the detection and quantification of nucleic acid. while the original technology is based on the amplification by polymerase chain reaction (pcr) of the target sequence followed by its detection using the invader technology, the current modification allows simultaneous pcr amplification and invader reaction. the pcr primers and the invader probes are designed to operate at the same temperature. this allows simpler design and faster resu ...201018718748
the influence of mineral trioxide aggregate on adaptive immune responses to endodontic pathogens in mice.this study assessed the influence of mineral trioxide aggregate (mta) on adaptive immune responses. balb/c mice were immunized with heat-killed fusobacterium nucleatum (fn) in mta or other control adjuvants, and serum igg responses to fn were measured. either fn- or peptostreptococcus anaerobius (pa)-reactive memory t cells (tm) were preincubated in vitro with/without mta and restimulated with fn or pa. tm proliferation and cytokine production were assessed. compared with control groups, immunog ...200818718367
antiviral, antibacterial and vitamin c-synergized radical-scavenging activity of sasa senanensis rehder extract.sasa senanensis rehder extract (se) showed slightly higher cytotoxicity against human squamous cell carcinoma cell lines and human glioblastoma cell lines, as compared with human oral normal cells (gingival fibroblast, pulp cell, periodontal ligament fibroblast), and was more cytotoxic to human myelogenous and t-cell leukemia cell lines. se showed a bacteriostatic effect on fusobacterium nucleatum and prevotella intermedia, but almost completely eliminated hydrogen sulfide (h2s) and methyl merca ...200818712174
[study of adherence of periodontal pathogens to collagen-treated hydroxyapatite].the ability of oral bacteria to adhere to tooth surface is associated with their pathogenicity. the objective of this study was to compare the ability of 4 strains of periodontal pathogens attaching to collagen-treated hydroxyapatite (c-ha) beads in order to evaluate the ability of the main periodontal pathogens to form the biofilm on root surface.200818705525
protein-lipid interactions with fusobacterium nucleatum major outer membrane protein foma: spin-label epr and polarized infrared spectroscopy.foma, the major outer membrane protein of fusobacterium nucleatum, was expressed and purified in escherichia coli and reconstituted from detergent in bilayer membranes of phosphatidylcholines with chain lengths from c(12:0) to c(17:0). the conformation and orientation of membrane-incorporated foma were determined from polarized, attenuated total reflection, infrared (ir) spectroscopy, and lipid-protein interactions with foma were characterized by using electron paramagnetic resonance (epr) spect ...200818642853
third molars and the efficacy of mechanical debridement in reducing pathogen levels in pregnant subjects: a pilot study.to assess the impact of mechanical debridement (scaling and root planing), without adjunctive therapy, on reducing the numbers of periodontal pathogens detected in pregnant subjects with and without visible third molars.200818634941
biochemical characterization of a glycosyltransferase homolog from an oral pathogen fusobacterium nucleatum as a human glycan-modifying enzyme.bacterial glycosyltransferases have drawn growing attention as economical enzymes for oligosaccharide synthesis, with their easy expression and relatively broad substrate specificity. here, we characterized a glycosyltransferase homolog (fnu_gt) from a human oral pathogen, fusobacterium nucleatum. bioinformatic analysis showed that fnu_gt belongs to the glycosyltransferases family ii. the recombinant fnu_gt (rfnu_gt) expressed in escherichia coli displayed the highest glycosylation activity when ...200818633282
periodontal status of patients with dentin dysplasia type i: report of three cases within a family.dentin dysplasia type i (ddi) is a rare hereditary disturbance of dentin formation. it is characterized by clinically normal-appearing crowns; obliteration of pulp chambers; and short, blunted and malformed roots that are commonly associated with periodontal attachment loss (pal). in this context, we report three cases within a family with similar clinical and radiographic features of ddi but with differing microbiologic and periodontal conditions.200818597615
comparative analysis of endodontic pathogens using checkerboard hybridization in relation to culture.the purpose of this study was to detect bacterial species and to quantify the total number of bacteria from samples of infected root canals before (s1) and after chemo-mechanical preparation using 2% chlorhexidine (chx) gel as auxiliary chemical substance (s2) and after 7 days of intracanal dressing (s3) to compare microbial changes.200818582327
effects of er:yag laser irradiation on biofilm-forming bacteria associated with endodontic pathogens in vitro.with the development of dental laser delivery systems that can enter into the root canals, it is possible to use er:yag lasers to remove the residual biofilm associated with infected root canals. we examined their effects against biofilms made of actinomyces naeslundii, enterococcus faecalis, lactobacillus casei, propionibacterium acnes, fusobacterium nucleatum, porphyromonas gingivalis, or prevotella nigrescens in vitro. after er:yag laser irradiation with energy densities ranging between 0.38- ...200818570988
differential gender effects of a reduced-calorie diet on systemic inflammatory and immune parameters in nonhuman primates.dietary manipulation, including caloric restriction, has been shown to impact host response capabilities significantly, particularly in association with aging. this investigation compared systemic inflammatory and immune-response molecules in rhesus monkeys (macaca mulatta).200818565132
rapid and simple detection of eight major periodontal pathogens by the loop-mediated isothermal amplification method.loop-mediated isothermal amplification (lamp) was applied to develop a rapid and simple detection system for eight periodontal pathogens: aggregatibacter (actinobacillus) actinomycetemcomitans, campylobacter rectus, eikenella corrodens, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia, treponema denticola and tannerella forsythia. primers were designed from the 16s ribosomal rna gene for each pathogen, and the lamp amplified the targets specifically and efficiently under ...200818565108
[relationship between herpesviruses and periodontal pathogenic bacteria in subgingival plaque].to evaluate the prevalence rates of human cytomegalovirus(hcmv) and epstein-barr virus-1(ebv-1) in subgingival plaque and analyze the relationship between herpesviruses, periodontal pathogenic bacteria and periodontal clinical parameters in chinese patients with aggressive periodontitis(agp).200818560463
experimental evidence supports the abscess theory of development of radicular cysts.the objective of this study was to experimentally induce inflammatory cysts in an animal model so as to test the hypothesis that radicular cysts develop via the "abscess pathway."200818554947
coaggregation between and among human intestinal and oral bacteria.coaggregation is believed to facilitate the integration of new bacterial species into polymicrobial communities. the aim of this study was to investigate coaggregation between and among human oral and enteric bacteria. stationary phase cultures of 10 oral and 10 enteric species, chosen on the basis of numerical and ecological significance in their respective environments together with their ease of cultivation, were tested using a quantitative spectrophotometric coaggregation assay in all possib ...200818554305
pcr-based identification of selected pathogens associated with endodontic infections in deciduous and permanent teeth.the aim of the present study was to evaluate the presence of the selected pathogens in samples from deciduous and permanent tooth root canals by using pcr method and to determine the association of these organisms with clinical symptoms.200818547832
in vitro evaluation of the effect of nicotine, cotinine, and caffeine on oral microorganisms.the aim of this in vitro study was to evaluate the effects of nicotine, cotinine, and caffeine on the viability of some oral bacterial species. it also evaluated the ability of these bacteria to metabolize those substances. single-species biofilms of streptococcus gordonii, porphyromonas gingivalis, or fusobacterium nucleatum and dual-species biofilms of s. gordonii -- f. nucleatum and f. nucleatum -- p. gingivalis were grown on hydroxyapatite discs. seven species were studied as planktonic cell ...200818535638
[periodontitis and cardiovascular disease].the prevalence of periodontitis and cardiovascular disease (cvd) is high. a mixed infectious biofilm etiology of periodontitis is known but not fully established in cvd. cofactors; smoking habits, stress, ethnicity, genetics, socioeconomics and age contribute to both diseases. the objectives of this report are to summarize factors in regards to cvd and periodontitis that are clinically relevant. the hypothesis behind a relationship between the two conditions can be founded in (i) shared infectio ...200818517068
the anti-endotoxic effects of the ksl-w decapeptide on escherichia coli o55:b5 and various oral lipopolysaccharides.host responses following the recognition of bacterial lipopolysaccharide can range from acute inflammation to septic shock. the aim of this study was to evaluate the ability of the ksl-w decapeptide to bind to and block the endotoxic effects of lipopolysaccharide.200818503516
photodynamic treatment of endodontic polymicrobial infection in vitro.we investigated the photodynamic effects of methylene blue on multispecies root canal biofilms comprising actinomyces israelii, fusobacterium nucleatum subspecies nucleatum, porphyromonas gingivalis, and prevotella intermedia in experimentally infected root canals of extracted human teeth in vitro. the 4 test microorganisms were detected in root canals by using dna probes. scanning electron microscopy showed the presence of biofilms in root canals before therapy. root canal systems were incubate ...200818498901
quercetin in a lotus leaves extract may be responsible for antibacterial activity.in the course of a search for chemotherapeutic agents inhibiting suspected peridontitis bacteria, extracted and purified substances from lotus leaf were identified by antimicrobial activity tests with use of the broth micro-dilution methods on 96-microwell plate. the antimicrobial activity of extracts was tested against five microorganisms, namely: actinobacillus actinomycetemcomitans y4, actinomyces viscosus 19246, porphyromonas gingivalis 33277, fusobacterium nucleatum 25586, and actinomyces n ...200818481022
[alterations of salivary flow rate and oral main pathogens in miniature pig with bilateral parotid gland atrophy].to observe the effect of bilateral parotid gland atrophy on the whole saliva flow rate and the growth of main oral pathogens in different sites of oral cavity.200718476559
candida dubliniensis and candida albicans display surface variations consistent with observed intergeneric coaggregation.adherence of yeasts to other microorganisms and epithelial cell surfaces is important in their colonization. comparative studies based on the coaggregation of candida dubliniensis versus candida albicans with fusobacterium nucleatum and other oral bacteria suggested differences in the surfaces of these yeasts. transmission electron microscopy was used to test the hypothesis that there are morphologic variations in the cell surface of these two species. c. dubliniensis type strain cd36 and c. alb ...199918473545
photodynamic therapy as adjunct to non-surgical periodontal treatment in patients on periodontal maintenance: a randomized controlled clinical trial.recent preclinical and clinical data have suggested the potential benefit of photodynamic therapy (pdt) in the treatment of periodontitis. however, currently, there are very limited data from controlled clinical trials evaluating the effect of pdt in the treatment of periodontitis. the aim of the present study was to evaluate the clinical and microbiological effects of the adjunctive use of pdt in non-surgical periodontal treatment in patients receiving supportive periodontal therapy. twenty-fou ...200918465191
ability of oral bacteria to induce tissue-destructive molecules from human neutrophils.the induction of tissue-destructive molecules from neutrophils by periodontopathic bacteria has been suggested as one of the mechanisms of periodontal destruction. the aim of this study was to determine whether the ability to stimulate neutrophils is an authentic characteristic of periodontopathic bacteria.200818449962
effects of age and oral disease on systemic inflammatory and immune parameters in nonhuman primates.this report evaluated systemic inflammatory and immune biomarkers in a cohort of macaca mulatta (rhesus monkeys) maintained as a large family social unit, including an age range from <1 year to >24 years. we hypothesized that the systemic host responses would be affected by the age, gender, and clinical oral presentation of the population, each contributing to inflammatory and immune responses that would reflect chronic oral infections. the results demonstrated that the prevalence and severity o ...200818448617
[prediction on the efficacy of fluoroquinolones for anaerobic infections using monte carlo simulation].various analysis based on pk-pd (pharmacokinetics-pharmacodynamics) theory were performed for prediction on the efficacy of antimicrobial agents for infectious diseases. however, there have been few reports when causative organisms would be anaerobic bacteria. although the antimicrobial activity of fluoroquinolones against anaerobes has been weak, gatifloxacin (gflx), moxifloxacin (mflx), and garenoxacin (grnx) are considered as anti-anaerobic quinolones in recent days. therefore, we investigate ...200718447205
a rare presentation of ventriculitis and brain abscess caused by fusobacterium nucleatum.anaerobic ventriculitis is rare, and usually seen in patients with predisposing factors such as otitis media, mastoiditis, sinusitis or recent neurosurgery. we report what we believe to be the first case of ventriculitis and brain abscess due to fusobacterium nucleatum infection in a man with no significant predisposing factors. he was successfully treated with antibiotic therapy.200818436604
impact of growth conditions on susceptibility of five microbial species to alkaline stress.the effects of different growth conditions on the susceptibility of five taxa to alkaline stress were investigated. enterococcus faecalis atcc 29212, streptococcus sobrinus omz 176, candida albicans atcc 90028, actinomyces naeslundii atcc 12104, and fusobacterium nucleatum atcc 10953 were grown as planktonic cells, allowed to adhere to dentin for 24 hours, grown as monospecies or multispecies biofilms on dentin under anaerobic conditions with a serum-enriched nutrient supply at 37 degrees c for ...200818436038
a microbiological profile of symptomatic teeth with primary endodontic infections.the aim of this study was to evaluate the composition of the microbiota of primary endodontic infections associated with symptomatic teeth. samples were collected by means of a #15 h-type file and 2 sterile paper points from 60 symptomatic (n = 30) or asymptomatic (n = 30) single-rooted teeth with necrotic pulp. the presence of 40 bacterial species was determined by the checkerboard dna-dna hybridization method. the species found in higher counts (x10(5)) in symptomatic cases were fusobacterium ...200818436031
serum antibodies to porphyromonas gingivalis chaperone htpg predict health in periodontitis susceptible patients.chaperones are ubiquitous conserved proteins critical in stabilization of new proteins, repair/removal of defective proteins and immunodominant antigens in innate and adaptive immunity. periodontal disease is a chronic inflammatory infection associated with infection by porphyromonas gingivalis that culminates in the destruction of the supporting structures of the teeth. we previously reported studies of serum antibodies reactive with the human chaperone hsp90 in gingivitis, a reversible form of ...200818431474
microbiological evaluation of primary endodontic infections in teeth with and without sinus tract.to examine the microbiological status of primary endodontic infections in teeth with and without a sinus tract.200818422583
[microbiological and biochemical characteristics of inflammatory tissues in the periodontium].to investigate bacterial populations in subgingival and supragingival plaque samples of patients with inflammatory periodontal diseases and activities of the lysosomal enzymes--lysozyme, alkaline phosphatase, and beta-glucuronidase--in peripheral venous blood, in gingival crevicular fluid, and mixed nonstimulated saliva.200818413987
bleeding on probing differentially relates to bacterial profiles: the oral infections and vascular disease epidemiology study.various bacterial species are differentially prevalent in periodontal health, gingivitis or periodontitis. we tested the independent associations between three bacterial groupings and gingival inflammation in an epidemiological study.200818400025
the lower genital tract microbiota in relation to cytokine-, slpi- and endotoxin levels: application of checkerboard dna-dna hybridization (cdh).in the present study the lower genital tract microbiota in asymptomatic fertile women (n=34) was identified and quantified by culturing vaginal secretions. also, vaginal and cervical samples were analyzed by a semiquantitative checkerboard dna-dna hybridization technique (cdh) based on genomic probes prepared from 13 bacterial species (bacteroides ureolyticus, escherichia coli, fusobacterium nucleatum, gardnerella vaginalis, mobiluncus curtisii ss curtisii, prevotella bivia, prevotella disiens, ...200818397461
role of porphyromonas gingivalis serb in gingival epithelial cell cytoskeletal remodeling and cytokine production.the serb protein of porphyromonas gingivalis is a had family serine phosphatase that plays a critical role in entry and survival of the organism in gingival epithelial cells. serb is secreted by p. gingivalis upon contact with epithelial cells. here it is shown by microarray analysis that serb impacts the transcriptional profile of gingival epithelial cells, with pathways involving the actin cytoskeleton and cytokine production among those significantly overpopulated with differentially regulate ...200818391005
bacterial profiles of root caries in elderly patients.culture-based studies have shown that streptococcus mutans and lactobacilli are associated with root caries (rc). the purpose of the present study was to assess the bacterial diversity of rc in elderly patients by use of culture-independent molecular techniques and to determine the associations of specific bacterial species or bacterial communities with healthy and carious roots. plaque was collected from root surfaces of 10 control subjects with no rc and from 11 subjects with rc. the bacterial ...200818385433
[detection of periodontal pathogens in coronary atherosclerotic plaques].to explore the possible correlation between coronary heart disease and periodontitis.200818380965
pyogenic liver abscess related to dental disease in an immunocompetent host.a 59-year-old man with poor oral hygiene presented to our hospital because of fever and chills. abdominal ultrasonography and enhanced computed tomography (ct) revealed a liver abscess. the patient had no history of immunodeficiency and we confirmed the patient had no immunologic abnormalities. blood culture revealed fusobacterium nucleatum, a bacterium commonly found in the oral cavity. even if a patient is immunocompetent, poor oral hygiene might be an independent risk factor for a pyogenic li ...200818379160
antimicrobial activity of sodium hypochlorite and chlorhexidine by two different tests.the aim of this study was to evaluate the antimicrobial capacity of sodium hypochlorite (1% and 5%) and chlorhexidine (0.12%, 0.5% and 1%) with or without the addition of organic material (bovine serum albumin, bsa) against some bacterial samples (staphylococcus aureus, enterococcus faecalis, escherichia coli, porphyromonas gingivalis and fusobacterium nucleatum) using two activity tests (contact and diffusion agar tests). in the contact test (first model), bacterial samples were kept in contact ...200818352899
thoracic vertebral actinomycosis: actinomyces israelii and fusobacterium nucleatum.actinomyces spp. are considered rare pathogens in today's medicine, especially with thoracic vertebral involvement. classic actinomycosis (50%) presents as an oral-cervicofacial ("lumpy jaw") infection. this report describes a case of spinal cord compression caused by actinomyces israelii with the coisolation of fusobacterium nucleatum. there are limited numbers of similar cases.200818337385
prevalence of periodontal pathogens in subgingival lesions, atherosclerotic plaques and healthy blood vessels: a preliminary study.previous studies have reported different periodontal bacteria in atherosclerotic lesions, but their involvement in plaque formation remains unclear. the aim of the present study was to investigate the presence of 20 periodontal bacteria in atherosclerotic samples and healthy blood vessels (used as controls) and to clarify their relationship in regard to clinical and bacteriological periodontal status.200818326058
genotypic diversity of anaerobic isolates from bloodstream infections.accurate species determination for anaerobes from blood culture bottles has become increasingly important with the reemergence of anaerobic bacteremia and prevalence of multiple-drug-resistant microorganisms. our knowledge of the taxonomical diversity of anaerobes that cause bloodstream infections is extremely limited, because identification historically has relied on conventional methods. over a 5-year period, we profiled anaerobic bacteremia at a large tertiary care hospital with 16s rrna gene ...200818322067
interactions between salivary bifidobacterium adolescentis and other oral bacteria: in vitro coaggregation and coadhesion assays.coaggregation assays were performed to investigate interactions between oral bifidobacterium adolescentis and other oral bacterial species. bifidobacterium adolescentis olb6410 isolated from the saliva of healthy humans did not coaggregate with actinomyces naeslundii jcm8350, streptococcus mitis ols3293, streptococcus sanguinis jcm5708, veillonella parvula atcc17745 or porphyromonas gingivalis ob7124, but it did coaggregate with fusobacterium nucleatum jcm8532. subsequent examination of biofilm ...200818312575
colonization pattern of periodontal bacteria in japanese children and their mothers.the purpose of this study was to determine the time of infection by anaerobic gram-negative rods associated with periodontal disease, and to clarify their transmission from mother to child.200818302616
aggregatibacter actinomycetemcomitans serotype f o-polysaccharide mediates coaggregation with fusobacterium nucleatum.intergeneric bacterial coaggregation may play an important role in plaque development.200818279180
smap29 congeners demonstrate activity against oral bacteria and reduced toxicity against oral keratinocytes.cathelicidins are antimicrobial peptides found in epithelial and mucosal tissues as well as the secondary granules of neutrophils. smap29, a sheep cathelicidin, has differential antimicrobial properties against various pathogens, including periodontal organisms. the purpose of this study was to evaluate the antimicrobial properties and cytotoxicity of smap29, smap28, and three congeners (smap18a, smap18d, and smap14a).200818279175
a case report of a pyogenic liver abscess caused by fusobacterium nucleatum in a patient with autosomal dominant polycystic kidney disease undergoing hemodialysis.pyogenic liver abscess (pla) is a process with significant morbidity and mortality and is a rare complication in an aisled way in patients with autosomal dominant polycystic kidney disease (adpkd). in addition to hepatic cyst infection, intracystic hemorrhage is another complication seen in adpkd patients; however, the liver parenchyma itself remains normal. a pla located in normal liver tissue in these kinds of patients has not been previously reported. fusobacterium nucleatum is an anaerobic b ...200818257820
in vitro antioxidant potential of medicinal plant extracts and their activities against oral bacteria based on brazilian folk medicine.this study aims to determine antibacterial activities of cocos nucifera (husk fiber), ziziphus joazeiro (inner bark), caesalpinia pyramidalis (leaves), aqueous extracts and aristolochia cymbifera (rhizomes) alcoholic extract against prevotella intermedia, porphyromonas gingivalis, fusobacterium nucleatum, streptococcus mutans and lactobacillus casei. the antioxidant activity and acute toxicity of these extracts were also evaluated.200818243157
genetic and biochemical characterization of oxa-63, a new class d beta-lactamase from brachyspira pilosicoli bm4442.brachyspira pilosicoli bm4442, isolated from the feces of a patient with diarrhea at the hospital saint-michel in paris, was resistant to oxacillin (mic > 256 microg/ml) but remained susceptible to cephalosporins and to the combination of amoxicillin and clavulanic acid. cloning and sequencing of the corresponding resistance determinant revealed a coding sequence of 807 bp encoding a new class d beta-lactamase named oxa-63. the bla oxa-63 gene was chromosomally located and not part of a transpos ...200818212108
microflora in oral ecosystems in subjects with radiation-induced hyposalivation.to analyse the microbial flora in specific oral sites in 13 dentate subjects, 6-8 months after completed radiation therapy (rt group) and in 13 matched controls.200818208474
relationship between campylobacter rectus and periodontal status during pregnancy.in a previous study, we showed that the growth of campylobacter rectus is stimulated by the presence of female sex hormones in the culture medium. in the present study, we examined the relationship between c. rectus levels in the saliva and the periodontal status of pregnant women.200818173799
proteomic analysis of ampicillin-resistant oral fusobacterium nucleatum.fusobacterium nucleatum represents one of the predominant anaerobic species in the oral microbiota. penicillin-resistant f. nucleatum have been isolated from intra- and extraoral infections. this study aimed to assess ampicillin resistance in f. nucleatum by investigating the synthesis of resistance-associated proteins.200818173796
stimulation of fusobacterium nucleatum biofilm formation by porphyromonas gingivalis.bacterial infection is a major cause of periapical periodontitis. eradication of these microorganisms from apical lesions is essential to the success of endodontic treatment. the aim of this study was to clarify the molecular interaction between fusobacterium nucleatum, porphyromonas gingivalis and other microorganisms associated with periapical periodontitis.200818173791
quantitative analysis of association between herpesviruses and bacterial pathogens in periodontitis.the development of human periodontitis may depend upon cooperative interactions among herpesviruses, specific pathogenic bacteria and tissue-destructive inflammatory mediators. this study sought to identify associations among human cytomegalovirus, epstein-barr virus and six putative periodontopathic bacteria in periodontitis lesions.200818086168
effect of photo-activated disinfection on endodontic pathogens ex vivo.to test the hypothesis that photo-activated disinfection (pad) has a bactericidal effect on pathogens inoculated in root canals, with emphasis on biofilm formation/destruction.200818081808
antimicrobial activity of mahonia aquifolium and two of its alkaloids against oral bacteria.extracts or alkaloids isolated from mahonia aquifolium exhibit antimicrobial activity against gram-positive and gram-negative bacteria, fungi, and protozoa. in this study the bacteriostatic and bacteriocidal activities of a m. aquifolium extract and two of its major alkaloids, berberine chloride and oxyacanthine sulphate, were tested in vitro against nine different oral bacteria. minimum inhibitory concentrations were in the range from < or = 0.0031% to 0.1993% for the m. aquifolium extract, fro ...200718072463
analysis of the antimicrobial activity of local anaesthetics used for dental analgesia.seven local anaesthetics and their active anaesthetic components [ultracaine d-s (articaine hydrochloride), carbostesin (bupivacaine hydrochloride), scandicaine (mepivacaine hydrochloride), xylonest (prilocaine hydrochloride), xylocaine (lidocaine hydrochloride), hostacaine (butanilicaine phosphate) and novocaine (procaine hydrochloride)] were tested for their antimicrobial activity against 311 bacterial strains from 52 different species and 14 candida albicans strains. the tested pathogens were ...200818065672
effect of rinsing with an essential oil-containing mouthrinse on subgingival periodontopathogens.studies have shown that the nature and amount of supragingival plaque can influence the composition of the contiguous subgingival flora. the purpose of this study was to investigate the effect of rinsing with an essential oil antimicrobial mouthrinse on levels of representative subgingival bacteria in subjects with mild to moderate periodontitis.200718062115
early soft tissue healing around one-stage dental implants: clinical and microbiologic parameters.despite the potential significance of early soft tissue healing to long-term outcomes, this aspect of one-stage dental implants has not been investigated. the purpose of this prospective study was to characterize clinical and microbiologic parameters of early soft tissue healing around dental implants placed following a one-stage protocol.200718062110
effect of tissue fluids on hydrophobicity and adherence of enterococcus faecalis to dentin.this in vitro study was carried out to determine (1) the hydrophobicity of selected oral bacteria, (2) the influence of growth media (saliva and serum) and mode of growth (planktonic or biofilm) on the hydrophobicity of enterococcus faecalis, and (3) the influence of growth media and conditioning fluids on the adherence of e. faecalis to dentin. the ability to bind to a hydrocarbon phase (xylene) was used as an index of relative hydrophobicity of cells. fluorescent microscopy-based technique was ...200718037050
[case report: positive signals from automated blood-culture system with negative direct gram examination].detection of positive haemoculture is usually managed by an automated system. when a bottle is detected positive but that the gram coloration does not reveal germs by direct examination, transfer onto chocolate blood agar generally allows to confirm or infirm bacteraemia. in light of a case of fusobacterium nucleatum bacteraemia, we discuss the opportunity of pairing it with an enrichment broth. m. n, hospitalized in the hepatogastroenterology department, runs a fever of undetermined origin. thr ...200718031953
improved diagnosis specificity in bone and joint infections using molecular techniques.the microbiological diagnosis of osteoarticular infections currently relies on microbiological cultures, to specifically target treatment. however, these conventional methods sometimes lack of sensitivity and of specificity to establish definitive diagnosis. this study was conducted to determine whether molecular method could improve bacterial bone and joint infection diagnosis.200718029022
effect of chlorhexidine/thymol and fluoride varnishes on dental biofilm formation in vitro.this study evaluated the effect of chlorhexidine/thymol (chx/t) and fluoride (f) varnishes on biofilm formation in vitro. hydroxyapatite discs coated with varnish were first immersed in saline for 0, 3, 7 or 14 d, then immersed in pasteurized saliva. the discs were incubated for 20 h with a bacterial suspension containing actinomyces naeslundii, fusobacterium nucleatum, streptococcus oralis, and veillonella dispar. uncoated discs were used as controls. growth of bacteria on the discs was evaluat ...200718028054
soluble cd14 discriminates slight structural differences between lipid as that lead to distinct host cell activation.soluble cd14 (scd14) in serum is known to sensitize host cells to lps. in the present study, the contributions of scd14 and lps-binding protein to a lipid a moiety from lps preparations of periodontopathogenic fusobacterium nucleatum sp. nucleatum were compared with that of escherichia coli-type synthetic lipid a (compound 506). f. nucleatum lipid a was identified to be a hexa-acylated fatty acid composed of tetradecanoate (c(14)) and hexadecanoate (c(16)), similar to dodecanoate (c(12)) and c(1 ...200718025213
[the development and in vitro experiment study of a bio-type root canal filling sealer using calcium phosphate cement].the purpose of this study is to develop a novel root canal filling sealers based on calcium phosphate cement (cpc), and to evaluate its physical-chemical properties and in vitro antibacterial activity on the predominant bacteria infecting root canal.200718004487
the inhibition of dentifrice containing the lotus leaf-derived inhibitor on periodontitis-related bacteria in vitro.to search for a substance in lotus leaf that could efficiently be used in toothpaste for preventing periodontal disease.200717992914
involvement of phospholipase d in regulating expression of anti-microbial peptide human beta-defensin-2.human beta-defensin expression correlates with differentiation in oral epithelium, and calcium ion, an important regulator of epithelial differentiation, plays a critical role in regulation of human beta-defensin-2 (hbd-2) mrna expression. phospholipase d (pld) also regulates epithelial differentiation. therefore, we examined the role of pld in hbd-2 up-regulation by cell wall extract of fusobacterium nucleatum and phorbol 12-myristate 13-acetate (pma), two known hbd-2 activators. we found that ...200817986621
antimicrobial effect of propolis and other substances against selected endodontic pathogens.the aim of this study was to determine the antimicrobial effect of ethanol extract of propolis (eep) and intracanal medicaments calcium hydroxide, camphorated paramonochlorophenol, and formocresol by means of the macrodilution method using the reinforced clostridial medium (rcm) and brucella and brain heart infusion media.200717964476
inhibitory effect of cranberry polyphenol on biofilm formation and cysteine proteases of porphyromonas gingivalis.the purpose of this study was to investigate the effects of cranberry polyphenol fraction on biofilm formation and activities of arg-gingipain and lys-gingipain in porphyromonas gingivalis.200717956474
innate immune responses of gingival epithelial cells to nonperiodontopathic and periodontopathic bacteria.we have previously reported different susceptibilities of periodontopathic and nonperiodontopathic bacteria to antimicrobial peptides and phagocytosis by neutrophils. differences between the two groups of bacteria may exist also in their ability to induce immune responses from the host. therefore, we evaluated the effects of various oral bacteria on innate immune responses by gingival epithelial cells.200717956462
antimicrobial profiles of prevotella spp. and fusobacterium nucleatum isolated from periodontal infections in a selected area of southern italy.in view of the increasing antibiotic resistance of anaerobic gram-negative bacteria, we determined the antimicrobial profile of 55 periodontal anaerobic gram-negative bacteria correlated with human infections, comprising 16 strains of fusobacterium nucleatum and 39 strains of prevotella spp. isolated from periodontal pockets of 26 adults suffering from chronic periodontitis. all the strains of f. nucleatum were susceptible to amoxicillin/clavulanic acid, doxycycline, metronidazole, moxifloxacin ...200717954025
improved accuracy in terminal restriction fragment length polymorphism phylogenetic analysis using a novel internal size standard definition.terminal restriction fragment length polymorphism (t-rflp) analysis is commonly used to analyze microbial communities, including oral microflora. however, accurate identification of terminal restriction fragment (t-rf) origins is prevented by unpredictable errors in sizing, thus necessitating the clone library analysis. to minimize sizing errors, we proposed optimizing the size definition of internal standards.200717949346
evaluation of the microbiota of primary endodontic infections using checkerboard dna-dna hybridization.the aim of this study was to evaluate the composition of the microbiota of primary endodontic infections in 111 selected cases of single-rooted teeth with necrotic pulp.200717949342
compressed mints and chewing gum containing magnolia bark extract are effective against bacteria responsible for oral malodor.flavors and natural botanic extracts are often used in chewing gum and compressed mints for breath freshening and relief of oral malodor. the oral malodor is a result of bacterial putrification of proteinaceous materials from food or saliva. in this study, magnolia bark extract (mbe) and its two main components, magnolol and honokiol, were evaluated by the minimum inhibition concentration (mic) test. the inhibitory effect of mbe mint was further evaluated by a kill-time assay study. in addition, ...200717949053
bacterial leakage along the implant-abutment interface of premachined or cast components.in recent clinical studies, contamination of the inner parts of dental implants through bacterial penetration along the implant components has been observed. the aim of the present in-vitro study was to investigate leakage of fusobacterium nucleatum through the interface between implants and premachined or cast abutments. both premachined (n=10) and cast (n=10) implant-abutment assemblies were inoculated with 3.0 microl of microbial inoculum. the assemblies were completely immersed in 5.0 ml of ...200817931833
the trimeric periplasmic chaperone skp of escherichia coli forms 1:1 complexes with outer membrane proteins via hydrophobic and electrostatic interactions.the interactions of outer membrane proteins (omps) with the periplasmic chaperone skp from escherichia coli are not well understood. we have examined the binding of skp to various omps of different origin, size, and function. these were ompa, ompg, and yaet (omp85) from escherichia coli, the translocator domain of the autotransporter nalp from neisseria meningitides, foma from fusobacterium nucleatum, and the voltage-dependent anion-selective channel, human isoform 1 (hvdac1) from mitochondria. ...200717928002
implant surface analysis and microbiologic evaluation of failed implants retrieved from smokers.the aim of this study was to evaluate the microbiota and surface of failed titanium dental implants from 4 manufacturers. twelve mobile dental implants were retrieved from 10 smokers after 3 to 10 years of functional loading. before implant removal, microbial samples were taken and evaluated using polymerase chain reaction. after implant removal, analyses of the failed implant surfaces were performed using scanning electron microscopy and energy-dispersive spectrometer x-ray. periodontal pathoge ...200717912965
antimicrobial effect of triclosan and triclosan with gantrez on five common endodontic pathogens.microbial control of the root canal system is one of the key objectives of root canal therapy. triclosan is a widely accepted broad spectrum antimicrobial agent proven to be effective against many gram-positive and gram-negative bacteria. triclosan acts by blocking bacterial fatty acid biosynthesis. the addition of gantrez copolymer has been shown to enhance the antimicrobial activity of triclosan. the purpose of this study was to determine the minimum inhibitory and bactericidal concentrations ...200717889698
[application of "microring an" to "level 1": presumptive identification of anaerobic gram-negative bacilli].commonly isolated anaerobic gram-negative rods (4 genus 64 strains), some other important gram-negative anaerobic species (9 genus 45 strains), and cigar-shaped clostridia (11 strains) were studied on their susceptibility patterns to 6 agents on "microring an". some modifications were made in the methods and interpretation of results. susceptibility patterns to erythromycin, rifampicin, colistin, benzylpenicillin, kanamycin, and vancomycin were following (sensitive [s], intermediate [i], resista ...200617822332
comparison of growth and susceptibility to sodium hypochlorite of mono- and dual-species biofilms of fusobacterium nucleatum and peptostreptococcus (micromonas) micros.this study compared growth and susceptibility to different concentrations of sodium hypochlorite (naocl) of mono- and dual-species biofilms of fusobacterium nucleatum or peptostreptococcus micros in vitro at 24 hours or 96 hours. a mann-whitney u test revealed that although at 24 hours dual-species biofilms had similar viable counts to those of monospecies biofilms (p > 0.001), they were more resistant to naocl (p < 0.001). at 96 hours, both microorganisms had higher viable counts and were more ...200717804319
functionalization of pvdf membranes with carbohydrate derivates for the controlled delivery of chlorhexidin.maltodextrin (mx) was fixed onto pvdf membranes in order to create a drug delivery guided tissue regeneration (gtr) device with controlled drug delivery properties. pvdf microporous membranes were treated by a mixture of mx and citric acid, resulting to an 18 wt% increase of the supports. mx grafted membrane could capture 103 mg/g chlorhexidin digluconate (digchx) instead of 1mg/g for a virgin membrane. a neutralization step was performed before the biological tests. viability tests confirmed th ...200717804290
the impact of a low-frequency chlorhexidine rinsing schedule on the subgingival microbiota (the teeth clinical trial).information on the efficacy of chlorhexidine (chx) rinsing on the subgingival microbiota is limited. this study tested if intermittent chx rinsing over 5 years had an impact on the subgingival microbiota.200717760545
hemoglobin and lps act in synergy to amplify the inflammatory response.vascular disruption and bleeding during periodontitis likely increase the levels of hemoglobin in gingival crevicular fluid. the aim of this study was to investigate the effect of hemoglobin on the inflammatory responses of human macrophages stimulated with lipopolysaccharides (lps) isolated from periodontopathogens. the production of interleukin-1 beta (il-1beta), il-6, il-8, and tumor necrosis factor alpha (tnf-alpha) by macrophages following challenges with porphyromonas gingivalis and fusoba ...200717720859
fusobacterium nucleatum induces fetal death in mice via stimulation of tlr4-mediated placental inflammatory response.intrauterine infection plays a pivotal role in preterm birth (ptb) and is characterized by inflammation. currently, there is no effective therapy available to treat or prevent bacterial-induced ptb. using fusobacterium nucleatum, a gram-negative anaerobe frequently associated with ptb, as a model organism, the mechanism of intrauterine infection was investigated. previously, it was shown that f. nucleatum induced preterm and term stillbirth in mice. fusobacterial-induced placental infection was ...200717675512
effects of glass ionomer and microfilled composite subgingival restorations on periodontal tissue and subgingival biofilm: a 6-month evaluation.this 6-month study evaluated the effects of resin-modified glass-ionomer cement (rmgi) and microfilled composite (mc) subgingival restorations on periodontal tissues and subgingival biofilm.200717668971
genome sequence of fusobacterium nucleatum subspecies polymorphum - a genetically tractable fusobacterium.fusobacterium nucleatum is a prominent member of the oral microbiota and is a common cause of human infection. f. nucleatum includes five subspecies: polymorphum, nucleatum, vincentii, fusiforme, and animalis. f. nucleatum subsp. polymorphum atcc 10953 has been well characterized phenotypically and, in contrast to previously sequenced strains, is amenable to gene transfer. we sequenced and annotated the 2,429,698 bp genome of f. nucleatum subsp. polymorphum atcc 10953. plasmid pfn3 from the stra ...200717668047
targeted profiling of oral bacteria in human saliva and in vitro biofilms with quantitative real-time pcr.an in vitro plaque model based on the use of human salivary bacteria and tooth-like surfaces was previously developed for studying the formation of oral biofilm and its use for pre-clinical testing of candidate antimicrobial or antiplaque agents. in this study, a quantitative taqman pcr assay (qpcr) was developed to compare the bacterial compositions of in vitro biofilms to parent saliva samples, and to determine the relative contributions of different species in the formation of the oral biofil ...200717653931
role of anaerobes in acute pelvic inflammatory disease.pouch of douglas aspirates were collected from 50 women with history and examination suggestive of acute pelvic inflammatory disease (pid) and 20 healthy women admitted for tubal ligation served as control. a total of 57 microorganisms were isolated from 37 patients out of 50 in study group. of 37 positive cultures 21(56.7%) were monomicrobial and 16(43.2%) were polymicrobial. most common symptom in study group was lower abdominal pain (90%), vaginal discharge (70%) and irregular bleeding (40%) ...200317643017
[lemierre syndrome]. 200717633598
development of a genus-specific pcr assay for the molecular detection, confirmation and identification of fusobacterium spp.a genus-specific polymerase chain reaction (pcr)-based assay is developed for the detection and identification of clinically relevant fusobacterium species, including f. nucleatum and f. necrophorum. two 16s ribosomal dna (rdna) primers, fuso1 (forward primer: 5'-gag aga gct ttg cgt cc-3' [17-mer]) and fuso 2 (reverse primer: 5'-tgg gcg ctg agg ttc gac -3' [18-mer]) are designed to target conserved regions of the 16s rdna gene for fusobacterium spp. subsequent proof-of-principle studies employin ...200717633142
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