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effect of aldose reductase inhibition on nerve conduction velocity and resistance to ischemic conduction block in experimental diabetes.the relationships between nerve polyol levels and both nerve conduction velocity (ncv) and resistance to ischemic conduction block (ricb) in streptozocin-induced diabetic rats were examined in two studies. in the first study, sciatic ncv and ricb of the tail nerve, assessed by measuring the time to disappearance of the nerve action potential after the tail was rendered ischemic, were measured in nondiabetic rats, untreated diabetic rats, and diabetic rats given statil, an aldose reductase inhibi ...19883133261
synthetic aci-reductones: 3,4-dihydroxy-2h-1-benzopyran-2-ones and their cis- and trans-4a,5,6,7,8,8a-hexahydro diastereomers. antiaggregatory, antilipidemic, and redox properties compared to those of the 4-substituted 2-hydroxytetronic acids.synthetic procedures for the elaboration of aci-reductones belonging to the 6- or 7-mono- or bis-substituted-3,4-dihydroxy-2h-1-benzopyran-2-ones (6-10) and their cis- and trans-4a,5,6,7,8,8a-hexahydro diastereomers (11, 12) are described. hexahydrobenzopyranone aci-reductones were conveniently prepared by using meldrum's synthon (2,2-dimethyl-1,3-dioxane-4,6-dione, 49). certain of these substances were evaluated for antilipidemic activity in the cholesterol-fed rat model, and all analogues were ...19883133477
effects of progressively increased doses of theophylline and of s 9795 on hemodynamics, blood gases and lung mechanics in dogs.we investigated the effects of progressively increased i.v. doses of theophylline and of the newly introduced methylxanthine derivative s 9795 on hemodynamics, blood gases and lung mechanics in 2 groups each of 8 pentobarbital sodium-anesthetized dogs. the maximum plasma levels that were attained were 56.6 +/- 3.4 micrograms/ml (mean +/- s.e.) for theophylline and 12 +/- 1.2 micrograms/ml for s 9795. theophylline increased heart rate and cardiac output, decreased arterial po2, increased o2 consu ...19883233049
the effect of nicotine on human endothelial cell release of prostaglandins and ultrastructure.the acute and sub-acute effects of nicotine at concentrations between 10(-9)m and 10(-2)m on human umbilical vein endothelial cell release of prostacyclin and prostaglandin e2 and the sub-acute effect on the endothelial cell ultrastructure have been examined. the acute effect of nicotine on prostaglandin release has been assessed by measuring release of prostacyclin and prostaglandin e2 following stimulation of confluent monolayers of endothelial cells with a23187 in the presence of nicotine. th ...19883134041
influence of radiant heat stress on respiratory water loss in new-born lambs.respiratory water loss, oxygen consumption and carbon dioxide production were measured during rest and during moderate radiant heat stress in 9 healthy unanaesthetized lambs, age 4-15 days. the method used is based on an open flow-through system with a mass spectrometer for gas analyses. respiratory water loss was 12.7 mg/kg min when the lamb was at rest and increased to 24.7 mg/kg min during heat stress. oxygen consumption was 13.9 ml/kg min at rest and remained unchanged during heat stress. th ...19883134055
analysis of phorbol ester stimulated human megakaryocyte development.megakaryocytes are relatively rare components of human bone marrow, making the study of their maturation difficult. phorbol esters can act as differentiating agents in a number of cell systems including murine megakaryocytes. we report the effects of phorbol esters on the previously described long-term human megakaryocytic leukemia cell culture, est-iu. while two nontransforming phorbols fail to affect these cells, the transforming phorbol 12-o-tetradecanoylphorbol-13-acetate (tpa) induces a phe ...19883134065
analysis of phorbol ester stimulated human megakaryocyte development.megakaryocytes are relatively rare components of human bone marrow, making the study of their maturation difficult. phorbol esters can act as differentiating agents in a number of cell systems including murine megakaryocytes. we report the effects of phorbol esters on the previously described long-term human megakaryocytic leukemia cell culture, est-iu. while two nontransforming phorbols fail to affect these cells, the transforming phorbol 12-o-tetradecanoylphorbol-13-acetate (tpa) induces a phe ...19883134065
[primary myelofibrosis showing megakaryoblastic crisis: a case report]. 19883164424
the function of human intercellular adhesion molecule-1 (icam-1) in the generation of an immune response.monoclonal antibody rr 1/1 directed against the putative lfa-1 ligand molecule intracellular adhesion molecule-1 (icam-1) was found to inhibit the t cell proliferative response to the antigen ppd. interestingly, the percentage of unstimulated monocytes which expressed icam-1 on their surface appeared to vary greatly from person to person although the majority of monocytes did express high levels of icam-1 within their cytoplasm and surface expression could be rapidly induced on most cells by adh ...19883257922
antibody synthesis specific for nonoral antigens in inflamed gingiva.in vitro experimentation indicates that periodontitis-associated bacteria contain potent polyclonal b-cell activators (pba). we reasoned that if pba were operative in vivo, plasma cells specific for nonoral antigens should be present in the inflamed gingival tissues, which are characterized by a plasma cell infiltrate. to test this, rabbits with experimental periodontitis were immunized in the hind legs with the histochemically detectable antigen horseradish peroxidase (hrp) or glucose oxidase ( ...19883257940
[analysis of the hypothermic action of etimizol].in chronic experiments on rabbits it was shown that the hypothermic effect appears in the series of antipheins after the addition of ethyl radical in the first position of imidazole ring. the hypothermic effect of etimizol is more pronounced in the brain than in the body, whereas caffeine equally increases the temperature of the brain and body.19883191969
[comparative study of the progestagenic action of 16 alpha, 17 alpha-cyclohexanoprogesterones (pregna-d'6-pentarans) when administered by different routes].a comparative study of the progestational activity of modified pentarans depending on the dose and the route of administration was carried out. it was shown that modification of the molecule of 16 alpha, 17 alpha-cyclohexanoprogesterones by introducing and additional double bond in positions 6, 7 and methyl group in position 6 provided a high gestational effect, being particularly pronounced at intramuscular administration.19883191979
specificity and retinotectal projections of quarter-eye fragments in xenopus laevis.three quarters of the eye anlage in xenopus embryos of stage 33/34 were eliminated in three different sets of experiments. the remaining quadrant originated from the nasoventral part of the retina, from its ventral portion, or from the temporo-ventral area of the retina. all the fragments developed into small eyes of normal shape. the retinotectal connections did not deviate from those found in the control groups, even though mirror-image duplication was fairly frequent. for all fragments the te ...19883077005
evolution of influenza polymerase: nucleotide sequence of the pb2 gene of a/chile/1/83 (h1 n1).the complete nucleotide sequence of the pb2 gene of influenza virus a/chile/1/83 (h1 n1) is presented. sequence comparison between a/chile pb2 protein and the known pb2 sequences of the influenza strains a/wsn/33 (h1 n1), a/pr/8/34 (h1 n1), a/nt/60/68 (h3 n2), a/kiev/59/79 (h1 n1), a/fpv/rostock/34 (h7 n1), and b/ann arbor/1/66 indicates extensive amino acid homology for the influenza a virus pb2 proteins. small clusters of basic amino acids are conserved in all pb2 proteins including the influe ...19883214273
outer membrane protein pattern of eubacterium plautii.the outer membrane sds-page pattern of eubacterium plautii was characterized by a large number of surface exposed low- and high-molecular-mass proteins. silver stainable carbohydrate was not present. the pattern was clearly distinct from those of outer membrane preparations of eubacterium saburreum and fusobacterium nucleatum. the results are compatible with a gram-positive cell wall structure in e. plautii.19883221197
characteristics of glutamate dehydrogenase, a new diagnostic marker for the genus fusobacterium.enzymes representative of carbohydrate and nitrogen metabolism were screened for their presence and activity amongst species of the genus fusobacterium. glutamate dehydrogenase (gdh) was reliably detected in all 25 strains studied. the ph profile of this enzyme and the dna base composition of selected strains were also determined. dna base composition of selected strains ranged between 28-32.9 mol% g + c. gdh was active between ph 7.5-11.5 but two ph profiles of activity, with optima at 9.5 and ...19883171543
a new expression system for mammalian cells based on putative replicator sequences of the mouse and a truncated thymidine kinase gene.we have constructed a new expression vector for mammalian cells. the vector contains a truncated tk gene for amplification under selective conditions, a sequence putatively supporting the replication of plasmid dna in eukaryotic cells (murine autonomously replicating sequence) and an expression cassette for the cdna to be studied. as a model cdna we have used that of human tissue-type plasminogen activator (t-pa). analysis of hirt supernatants and chromosomal dna from l cells, prepared six weeks ...19883149608
[effect of eicosanoids on the accumulation of cholesterol and the proliferation of subendothelial cells in the human aorta].the effect of prostacyclin and stable thromboxane analog a2 on endothelial culture of human aorta was studied. it was shown that prostacyclin inhibited accumulation of cholesterol in the cells and their proliferation, while thromboxane exhibited an opposite effect. calcium antagonists potentiated effects of prostacyclin and inhibited them in respect to thromboxane. screening of a number of synthetic agents affecting arachidonic acid metabolism was carried out. it was found that lipoxygenase inhi ...19883122858
regional localization of cd18, the beta-subunit of the cell surface adhesion molecule lfa-1, on human chromosome 21 by in situ hybridization.the gene coding for the beta-subunit of the cell surface adhesion glycoprotein lfa-1 has been localized to the tip of the long arm of chromosome 21 at 21q22.1-qter.19883073708
anaerobic infections in the head and neck region.anaerobic bacteria form the predominant flora of the oral cavity, outnumbering facultative organisms by 10-1,000: 1. the type of anaerobic bacteria and their concentration depend on the anatomical site and the degree of anaerobiosis in the different sites in the mouth. three groups of anaerobic bacteria inhabit the oral cavity; the strict anaerobes, the moderate anaerobes, and the microaerophilic group of organisms. the majority of anaerobic bacterial infections occurring in the region of the mo ...19883074469
localization of the kras2 oncogene in the domestic rabbit (oryctolagus cuniculus).the kras2 gene was localized in the rabbit by chromosomal in situ hybridization, using a 3h-labeled human cdna probe. there were 201 silver grains on 144 metaphase spreads; 12.9% of the grains resided on chromosome 16, and 54% of these grains were located close to the centromere at 16p11----q11. statistical analysis indicated that labeling at this region represents a significant deviation from a random distribution and thus provided evidence for the assignment of kras2 to 16p11----q11. in additi ...19883073912
production of immunoreactive growth hormone by mononuclear leukocytes.in the present study, we evaluated whether mononuclear leukocytes could synthesize and secrete growth hormone (gh) in vitro. by using rna slot blot analysis, we detected maximum spontaneous levels of specific gh mrna in the cytoplasm of rat leukocytes after a 4-h incubation. northern gel analysis demonstrated that the specific leukocyte gh rna was polyadenylated and had a molecular mass of 1.0 kb. further studies using immunofluorescence, antibody affinity chromatography, and sephacryl gel filtr ...19883044906
[mass-spectrometric determination of specificity of proteinases].analysis of methods of the proteinase substrate specificity determination revealed that michaelis constants found from the rates of hydrolysis of small synthetic substrates cannot be a good measure of selectivity, especially for proteinases of broad specificity used for hydrolysis of substrates of high molecular mass with multiple cleavage sites. mass-spectrometric studies (eriad) of the hydrolysis of the insulin b chain with trypsin, chymotrypsin, and proteinase i showed that monitoring of the ...19883056418
mechanism of proline-specific proteinases: (i) substrate specificity of dipeptidyl peptidase iv from pig kidney and proline-specific endopeptidase from flavobacterium meningosepticum.the substrate specificity of dipeptidyl peptidase iv (dipeptidyl peptide hydrolase, ec 3.4.14.5) from pig kidney and proline-specific endopeptidase from flavobacterium meningosepticum, was investigated with a series of n-terminal unprotected (dipeptidyl peptidases iv) and succinylated dipeptidyl-p-nitroanilides (proline-specific endopeptidase). both enzymes are specific for the s configuration of the amino-acid residue in p1 and p2 position if the penultimate residue is proline. in the case of a ...19882896517
[cryopreservation of early human embryo stages].a short review of freezing procedures applied to early human embryos is given. it is noted that human embryos survived freezing and thawing at a developmental stage of 1. cell to blastocyst. but it seems to be necessary to use for any developmental stage of early embryo a special freezing and thawing method. embryo survival is correlated with their morphologic features where as neither age of embryos nor developmental stage were involved in freezing and thawing ability.19883071049
involvement of prostaglandins in histamine-induced fluid and electrolyte secretion by rat colon.histamine increased the transmural potential difference across rat colon in-vivo and induced a net secretion of fluid. both effects were inhibited by indomethacin. histamine increased the potential difference and short-circuit current, and reduced tissue resistance in colonic sheets in-vitro. this response was reduced in the absence of chloride in the bathing medium or in the presence of serosal frusemide, suggesting that histamine stimulated electrogenic chloride secretion by the colon. the ris ...19882897440
the effect of metoclopramide treatment on diabetic cystoparesis. 19882897479
neurotransmitters, cytokines, and the control of alveolar bone remodeling in orthodontics.this article described research aimed at testing the hypothesis that tissue remodeling during orthodontic tooth movement is modulated, at least in part, by factors derived from the nervous and vascular (immune) systems. specifically, the neurotransmitters sp and vip and the cytokines il-1 alpha and il-1 beta were localized immunohistochemically in paradental tissues of cat canines that had been treated by the application of an 80 g tipping force for 1 hour to 14 days. increased staining (concent ...19882900159
oestrus phases of the syrian hamster. 19882976222
localization of the mdx mutation within the mouse dystrophin gene.we have mapped human and mouse x chromosome-specific genomic and cdna probes through an interspecies mus musculus/spretus pedigree which contains the mdx mutation. the positions of these markers relative to one another and to the mdx mutation were delineated. using probes corresponding to segments of the human duchenne muscular dystrophy (dmd) gene transcript, the position of a cross-hybridizing mouse equivalent gene (mdmd) was located. in more than 200 animals mapped, three were identified whic ...19882903046
antiviral chemoprophylaxis for cytomegalovirus infection. 19882849602
importance of pulmonary blood volume and aortic blood pressure in regulation of left ventricular function. 19882900297
comparative efficacy of ceftriaxone and rifampicin in eradicating pharyngeal carriage of group a neisseria meningitidis.during an outbreak of meningococcal meningitis in saudi arabia, oral rifampicin (four doses in two days) was compared with a single intramuscular dose of ceftriaxone for prophylaxis in family contacts of patients with meningococcal disease. pharyngeal samples were taken for culture before and 1 and 2 weeks after administration. both follow-up cultures indicated that ceftriaxone was significantly more effective. at 1 week the eradication rates for ceftriaxone and rifampicin were 97% and 75%; at 2 ...19882897515
a substituted thieno[3.4-d]imidazole versus substituted benzimidazoles as h+, k+-atpase inhibitors.s 3337, 2-(2-ethylaminobenzylsulfinyl)-5,6-dimethoxybenzimidazole, and s 1924, 2-(5-methyl-2-picolylsulfinyl)-1h-thieno[3.4-d]imidazole, are members of new classes of h+, k+-atpase inhibitors. their effects on h+, k+-atpase and 14c-aminopyrine uptake in gastric glands were studied as well as in vivo in pylorus-ligated rats, stomach-lumen-perfused rats and heidenhain pouch dogs. their inhibitory effects were compared with the effect of omeprazole. in pylorus-ligated rats the two compounds showed ...19882841691
immunobiological activities of a porin fraction isolated from fusobacterium nucleatum atcc 10953.from fusobacterium nucleatum atcc 10953 cell envelope fraction whose inner membranes had been removed by treatment with sodium n-lauroyl sarcosinate, an outer membrane protein (37,000 mr in a native state) was prepared by extraction with lithium dodecyl sulfate. the protein thus obtained showed distinct porin activity, namely, the ability to form hydrophilic diffusion pores by incorporation into the artificial liposome membrane. the porin fraction exhibited strong immunobiological activities in ...19882831155
evidence for a na+/h+ antiport in stomach smooth muscle cells.single smooth muscle cells were isolated from circular muscle of the canine gastric corpus by collagenase incubation. cytoplasmic ph (phi) of these cells was measured fluorometrically using the trapped dye 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein. cells were examined for their na+/h+ exchange activity after intracellular acidification. cells acid-loaded by propionate exposure, the nh4+ prepulse technique or suspension in a na+-depleted medium regained almost normal phi upon exposure to a n ...19882831889
insulin-like growth factor i binding in hepatocytes from human liver, human hepatoma, and normal, regenerating, and fetal rat liver.insulin-like growth factor-i (igf-i) in human hepatoma cells (hep-g2) has, in addition to its effect on cell growth, short-term metabolic effects acting through its own receptor. we have demonstrated that normal human hepatocytes, compared with hep-g2 cells, have virtually no igf-i binding sites. because the rate of growth is the major difference between the hepatoma and the normal liver, we asked if normal liver might express igf-i binding sites under physiologic growth conditions. indeed, wher ...19882832449
preparation by direct metal exchange and kinetic study of active site metal substituted class i and class ii clostridium histolyticum collagenases.active site metal substitutions for both gamma- and zeta-collagenases from clostridium histolyticum have been made by direct metal exchange. the incubation of co(ii), cu(ii), ni(ii), cd(ii), and hg(ii) with these native collagenases results in changes in activity that parallel those observed for the reconstitution of the respective apoenzymes with these metal ions. for both collagenases, the exchange reactions with co(ii) and cu(ii) are complete within 1 min. however, the changes in activity obs ...19882849992
high-performance liquid chromatographic separation and immunological characterization of soluble bovine viral diarrhea virus antigen.sodium dodecyl sulphate-polyacrylamide gel electrophoresis and western blot analysis of the protein extracts from bovine viral diarrhea virus (bvdv, singer strain) infected primary calf testicle cells (soluble antigen) showed the presence of four virus specific polypeptides of 105, 90, 84 and 67 kilodaltons (kd) the 84-kd being the most abundant. anion-exchange high-performance liquid chromatography (hplc) of soluble antigen separated the virus specific polypeptides in individual peaks while the ...19882832429
ecology of colorado tick fever. 19882849373
[role of alpha-1 and alpha-2-adrenoreceptors in achieving the compensatory response of the hemostasis system to plasmin]. 19882849566
[immunochemical study of the lipopolysaccharides of yersinia enterocolitica serovars o5 and o5.27].y. enterocolitica lipopolysaccharides (lps), serovars o5 and o5.27, have similar antigenic specificity. the lps of serovar o5.27 has been shown to contain no factor o27. residual alpha-l-rhamnose, glycosylated by d-xylulose, has been found to play an important role in the formation of factor o5, common for both serovars.19882469272
identification of a galactose-binding lectin on fusobacterium nucleatum fn-2.a previous study has suggested that fusobacterium nucleatum fn-2 contains a galactose-binding protein (lectin) on the cell surface (p. a. murray, v. matarese, c. i. hoover, and j. r. winkler, fems microbiol. lett. 40:123-127, 1987). in the present study, the molecular specificity and size of this lectin were investigated by several techniques. whole-cell affinity chromatography with asialofetuin covalently coupled to sepharose 6mb demonstrated that 81% of 3h-labeled f. nucleatum were specificall ...19882451642
thermochemosensitivity: augmentation by hyperthermia of cytotoxicity of anticancer drugs against human colorectal cancers, measured by the human tumor clonogenic assay.thermochemosensitivity was examined in vitro by a human tumor clonogenic assay (htca) using specimens obtained surgically from 43 patients with colorectal cancer. we found that the percentages of patients whose cells showed higher sensitivity (greater than 70% inhibition of colony formation) to hyperthermia alone were 29.6 and 55.6% at 42 and 43 degrees c each for 1 h, respectively. similarly, percentages of patients whose cells were sensitive to drugs alone were 13.8, 24.1 and 48.3%, for cis-di ...19883368200
comparative studies on the central effects of the angiotensin ii analogue (sar1 azaval3 ile8) at ii.the effects of the newly-synthesized at ii analogue (sar1 azaval3 ile8) at ii were investigated in comparison with the octapeptide at ii and the analogue saralazin (sar1 ala8) at ii, using intracerebroventricular administration, with respect to the following parameters: the level of biogenic monoamines (da, na and 5-ht) and the metabolites hva and 5-hiaa in mouse forebrain; the behaviour of the animals--cataleptogenic actions of mice, ptz convulsive--seizure threshold in mice, apomorphine stereo ...19883407412
influence of coenzyme q10 on doxorubicin uptake and metabolism by mouse myocardial cells in culture. 19883416371
cauda equina syndrome secondary to disseminated zygomycosis.we describe a case of cauda equina syndrome secondary to disseminated zygomycosis. a 52-year-old man had myelodysplastic syndrome, progressive weakness of the lower extremities, and incontinence. neuroradiological findings were consistent with lumbar stenosis and probable disk herniation. a lung lesion was also discovered on the chest roentgenogram. the patient's condition rapidly deteriorated after surgical decompression. attempts at determining other etiologies were unsuccessful. at autopsy, d ...19883193592
functional and structural alterations of the nervous system induced by avian retrovirus rav-7.chickens infected as embryos with rav-7 developed neurological signs including ataxia, lethargy, and imbalance. evoked spinal cord potentials for rav-7 infected sc chickens were considerably slower (64.8 m/s) than for uninfected sc (103.4 m/s), genetically hypothyroid (os) (93.9 m/s) or special c (95.1 m/s) chickens. conduction velocity measurements of sciatic nerves showed normal values for all the chickens examined in this study. histopathological studies revealed non-suppurative meningoenceph ...19883193874
functional and structural alterations of the nervous system induced by avian retrovirus rav-7.chickens infected as embryos with rav-7 developed neurological signs including ataxia, lethargy, and imbalance. evoked spinal cord potentials for rav-7 infected sc chickens were considerably slower (64.8 m/s) than for uninfected sc (103.4 m/s), genetically hypothyroid (os) (93.9 m/s) or special c (95.1 m/s) chickens. conduction velocity measurements of sciatic nerves showed normal values for all the chickens examined in this study. histopathological studies revealed non-suppurative meningoenceph ...19883193874
the comparative cytotoxicity of periodontal bacteria.the direct cytotoxicity of sonic extracts (se) from nine periodontal bacteria for human gingival fibroblasts (hgf) was compared. equivalent dosages (in terms of protein concentration) of se were used to challenge hgf cultures. the cytotoxic potential of each se was assessed by its ability to (1) inhibit hgf proliferation, as measured by direct cell counts; (2) inhibit 3h-thymidine incorporation in hgf cultures; or (3) cause morphological alterations of the cells in challenged cultures. the highe ...19883204481
[nursing research based on human engineering: the home environment]. 19883221700
a combined tension band and lag screw technique for fixation of olecranon osteotomies.a combined tension band and lag screw technique for fixation of olecranon osteotomies was used in six canine clinical patients weighing 4.5 to 19 kg. after the proximal part of the ulnar shaft was exposed, a screw hole was drilled and tapped just cranial to the caudal cortex of the olecranon. an osteotomy was performed and the hole in the olecranon fragment was overdrilled to form a gliding hole. for reconstruction, the olecranon was reduced anatomically and compressed with a screw placed in lag ...19883232327
human pharmacological investigation of a human recombinant tumor necrosis factor preparation (pac-4d) a phase-i trial.15 patients aged between 24 and 66 years with 10 different malignant tumor diseases were treated with a recombinant human tumor necrosis factor preparation pac-4d in a phase-i trial. the starting dose was 10(5) u pac-4d as an intravenous short infusion. the maximally tolerable dose is around 18 x 10(5) u/m2. as the main clinical side effects were observed: fever, chills, hypertension with subsequent hypotension, lethargy, transient somnolence, headache, neurological deficiency symptoms, nausea a ...19883377652
isofluorane inhibits early mouse embryo development in vitro.in vitro development of 2-cell mouse embryos to the blastocyst stage was used to assess the toxicity of isofluorane, nitrous oxide, fentanyl, and meperidine. isofluorane at concentrations similar to those employed during human oocyte recovery for ivf significantly inhibited mouse embryo development. since the other agents were without effect, balanced anesthesia with nitrous oxide and narcotics may be preferable to isofluorane for ivf and gift.19883335266
isofluorane inhibits early mouse embryo development in vitro.in vitro development of 2-cell mouse embryos to the blastocyst stage was used to assess the toxicity of isofluorane, nitrous oxide, fentanyl, and meperidine. isofluorane at concentrations similar to those employed during human oocyte recovery for ivf significantly inhibited mouse embryo development. since the other agents were without effect, balanced anesthesia with nitrous oxide and narcotics may be preferable to isofluorane for ivf and gift.19883335266
recovery of anaerobic bacteria from clinical specimens in 12 years at two military hospitals.examination of 15,844 clinical specimens submitted over 12 years (1973 to 1985) to the anaerobic microbiology laboratories in two military hospitals demonstrated the recovery of anaerobic bacteria in 4,458 (28.1%) specimens. the specimens yielded 6,557 anaerobic isolates (1.47 isolates per specimen). bacteroides spp. accounted for 43% of all isolates; anaerobic gram-positive cocci, 26%; clostridium spp., 7%; and fusobacterium spp., 4%. bacteroides spp. predominated in abscesses, obstetrical and ...19883384929
lack of correlation between anatomic infarct size and ventriculographic variables following coronary occlusion in closed-chest dogs.1. although the effects of therapeutic interventions upon infarct size are frequently assessed on the basis of left ventricular ejection fraction and segmental contraction, the correlation of these variables with infarct size has not been thoroughly evaluated. to explore such relationships, we occluded the left anterior descending coronary artery of 22 closed-chest dogs and performed contrast ventriculography one week later. regional myocardial function was evaluated by a computer system in 60 r ...19883248241
a combined tension band and lag screw technique for fixation of olecranon osteotomies.a combined tension band and lag screw technique for fixation of olecranon osteotomies was used in six canine clinical patients weighing 4.5 to 19 kg. after the proximal part of the ulnar shaft was exposed, a screw hole was drilled and tapped just cranial to the caudal cortex of the olecranon. an osteotomy was performed and the hole in the olecranon fragment was overdrilled to form a gliding hole. for reconstruction, the olecranon was reduced anatomically and compressed with a screw placed in lag ...19883232327
atypical invasive external otitis from aspergillus.we report a rare case of invasive external otitis caused by aspergillosis in an elderly nondiabetic patient. amphotericin b therapy was curative. atypical features of the presentation delayed diagnosis. early use of tissue biopsy and culture to guide prompt initiation of therapy is recommended. the clinical spectrum and microbiology of invasive aspergillosis are also reviewed.19883408569
atypical invasive external otitis from aspergillus.we report a rare case of invasive external otitis caused by aspergillosis in an elderly nondiabetic patient. amphotericin b therapy was curative. atypical features of the presentation delayed diagnosis. early use of tissue biopsy and culture to guide prompt initiation of therapy is recommended. the clinical spectrum and microbiology of invasive aspergillosis are also reviewed.19883408569
[in vitro organ cultures of human embryonic and fetal thymus]. 19883168902
evidence of estrogen receptors in normal human osteoblast-like cells.in seven strains of cultured normal human osteoblast-like cells, a mean of 1615 molecules of tritium-labeled 17 beta-estradiol per cell nucleus could be bound to specific nuclear sites. the nuclear binding of the labeled steroid was temperature-dependent, steroid-specific, saturable, and cell type-specific. these are characteristics of biologically active estrogen receptors. pretreatment with 10 nanomolar estradiol in vitro increased the specific nuclear binding of progesterone in four of six ce ...19883388021
the synergistic effect of cefotaxime and desacetylcefotaxime against clinical isolates of anaerobic bacteria.the synergistic interaction of cefotaxime and desacetylcefotaxime against 187 clinically significant anaerobic organisms was investigated. fusobacterium nucleatum, actinomyces odontolyticus, propionibacteria, lactobacilli, peptostreptococci, streptococcus intermedius and veillonella were sensitive to cefotaxime. both eubacterium lentum and streptococcus morbillorum were resistant. the susceptibility of the clostridia varied from 0.125 to greater than 256 mg/l; only 20% of species demonstrated sy ...19883396488
microbial flora of surface versus core tonsillar cultures in recurrent tonsillitis in children.surface and 'core' tonsillar specimens were collected from 60 children, between 2 and 14 years of age, suffering from recurrent tonsillitis, in order to establish the reliability of surface tonsillar swabbing technique and to define the most frequently involved aerobic and anaerobic microorganisms. from the qualitative point of view, the same mixed aerobic and anaerobic flora were obtained in both samples thus demonstrating the reliability of the surface swabbing technique. from the quantitative ...19883397235
immunobiological properties of lipopolysaccharides isolated from fusobacterium nucleatum and f. necrophorum.lipopolysaccharides (lpss) were isolated from fusobacterium nucleatum atcc 10953 and f. necrophorum atcc 25286 by the hot phenol/water procedure. f. nucleatum lps was composed of 16% (w/w) carbohydrate, 10% (w/w) hexosamine and 40% (w/w) fatty acid, while f. necrophorum lps was composed of 26% (w/w) carbohydrate, 12% (w/w) hexosamine and 28% (w/w) fatty acid. these lps preparations induced mitogenic responses in spleen cells of balb/c, balb/c (nu/nu) and c3h/hen mice, and these responses were su ...19883265430
production of mouse monoclonal antibodies for the analysis of idiotypes in serum of patients with chronic lymphatic leukaemia.in this report a simple procedure for the production of murine monoclonal antibodies (moab) against the idiotype of malignant b cells is described. mice were immunized with lymphoid cells from patients with b-cell chronic lymphocytic leukaemia (b-cll). after fusion of the spleen cells, hybridoma supernatants were screened for anti-idiotypic moab in elisa with immunoglobulins obtained from tumour-cell lysates, xenohybridomas and patients' sera. the anti-idiotypic moab were used to study tumour ce ...19883257881
oscillations and chaos in epidemics: a nonlinear dynamic study of six childhood diseases in copenhagen, denmark.using traditional spectral analysis and recently developed non-linear methods, we analyze the incidence of six childhood diseases in copenhagen, denmark. in three cases, measles, mumps, rubella, the dynamics suggest low dimensional chaos. outbreaks of chicken pox, on the other hand, conform to an annual cycle with noise superimposed. the remaining diseases, pertussis and scarlet fever, remain problematic. the real epidemics are compared with the output of a monte carlo analog of the seir model f ...19883266037
on the prevention and treatment of calcification disorders of old age.from several investigations it is known that magnesium oxide or hydroxide therapy causes a considerable delay of kidney stone recurrence in man. further, it is known that the mortality of populations due to cardiovascular disease in areas with hard drinking water containing more magnesium and having a higher mg/ca ratio than soft water, is considerably lower than in soft water areas. in the present study a physiological model for the homeostasis of calcium and phosphate is given. it is shown tha ...19883285142
piezoelectric polymer curvature sensor for measurement of regional curvature radius of lv wall.to evaluate regional myocardial function, we developed a curvature sensor for direct and instantaneous measurement of the regional curvature radius of the lv wall. the sensor is a bimorph of two sheets of thin piezoelectric polymer film. the relation between output voltage of the sensor and the reciprocal of the known curvature radius has been shown to be linear. in anesthetized dogs, we inserted the curvature sensor into the subepimyocardium with a specially designed introducer. special care wa ...19883364584
enzymatic capacity and protein synthesis in human muscle postoperatively.the glycolytic and aerobic oxidative capacity in skeletal muscle was investigated to reveal if the decrease seen in muscle protein synthesis is accompanied by a fall in the enzymatic capacity to oxidize substrates. six patients undergoing elective abdominal surgery were investigated by percutaneous muscle biopsies taken before surgery and on the first and third postoperative days. protein synthesis as assessed by the polyribosome concentration was 40% lower on the third day postoperatively than ...19883144105
inhibitory effect of gnrh on isolated rat uterine muscle contractility.the effect of gnrh upon uterine contractions of both non-pregnant and pregnant rats was examined in vitro. in the non-pregnant rat uterus, gnrh inhibited in a concentration-and-time dependent manner the contractions induced by acetylcholine and oxytocin, but not those caused by bradykinin and angiotensin ii. gnrh also inhibited the rhythmic contractions induced by oxytocin in uterine strips from late pregnant rats. these findings show that gnrh has a direct inhibitory effect on the rat uterine c ...19883290605
induction of gene amplification by arsenic.arsenic is a well-established carcinogen in humans, but there is little evidence for its carcinogenicity in animals and it is inactive as an initiator or tumor promoter in two-stage models of carcinogenicity in mice. two arsenic salts (sodium arsenite and sodium arsenate) induced a high frequency of methotrexate-resistant 3t6 cells, which were shown to have amplified copies of the dihydrofolate reductase gene. the ability of arsenic to induce gene amplification may relate to its carcinogenic eff ...19883388020
[immunomodulating properties of khlotazol].a nonsteroidal anti-inflammatory agent khlotazol administered to rats in doses of 40-80 mg/kg for 5 days stimulated synthesis of igm- and igg-antibodies to ram erythrocytes and increased the number of antibody-forming cells (afc) and rosette-forming cells (rfc) in the spleen. when the doses were increased to 160-320 mg/kg, the stimulating effect of the drug on the production of hemagglutinins retained but dose-dependent inhibition of the number of afc and rfc was found. when administered in a do ...19883410037
influence of increasing concentrations of ethanol on food and water intake, body weight, and wheel-running of male sprague-dawley rats.male sprague-dawley rats were used to study the influence of increasing concentrations of ethanol on wheel-running, food and water intake and body weight. animals were housed individually in screen-bottom cages (n = 12) or in activity wheel cages (n = 12). half the animals in each activity condition received an ethanol-free liquid diet, and the remainder received liquid diet in which the concentration of ethanol was increased by 5% of energy intake per week to a maximum of 35%, at the expense of ...19883413194
the preparation and characterization of cr(iii) and co(iii) complexes of gdp and gtp and their interactions with avian phosphoenolpyruvate carboxykinase.the exchange inert coordination complexes, cr(h2o)4gdp, cr(h2o)4gtp, cr(nh3)4gdp, cr(nh3)4gtp, co(nh3)4gdp, and co(nh3)4gtp have been synthesized and characterized. the lambda and delta coordination isomers of cr(h2o)4gdp, cr(nh3)4gdp, and the four cr(h2o)4gtp isomers have been separated by reverse phase hplc and characterized by their cd spectra. while the isomers of co(nh3)4gtp have not been successfully separated, 31p nmr spectroscopy reveals the presence of the lambda and delta forms. the co ...19883346664
common genetic pools between human populations.a further inquiry on modern human origins, based on common genetic pool surveys of rigorously selected population samples and highly informative immunological polymorphisms, provides new evidence of an occidental-oriental population split as the origin of human gene pool divergences. the most likely ancestral genetic profile is discussed in the context of the debate raised by preliminary dna restriction fragment length polymorphism studies, which contradict the conclusions drawn from classical b ...19883422217
new attachment formation following controlled tissue regeneration using biodegradable membranes.the present study evaluated the potential for reestablishment of connective tissue attachment after exclusion of the gingival connective tissue and epithelium during healing after periodontal surgery in a dog model. buccal mucoperiosteal flaps were elevated in all four premolar regions in two dogs. the buccal bone was reduced to approximately 25% of its original level on 24 roots. before the elevated flaps were replaced and sutured, membranes were adjusted to cover the exposed root surfaces. on ...19883422287
an in vitro model to study bacterial invasion of periodontal tissues.in periodontal disease, the abilities of bacteria to adhere to and degrade in vivo basement membranes should be considered as two of the rate-limiting steps for the potential active or passive invasion of gingival connective tissues. to study these mechanisms in greater detail, we used the pf hr-9 basement-membrane-like matrix to establish an in vitro model of bacterial invasion and degradation. three gram-negative anaerobic periodontopathic organisms, bacteroides gingivalis, fusobacterium nucle ...19883422291
[pharmacological correction of experimental alcoholic embryopathy].in the experiments on the progeny of ethanol-exposed rats it was shown that consumption of 15% alcohol instead of water during pregnancy resulted in the worsening of shuttlebox avoidance learning and decrease in succinate dehydrogenase activity in the visual and sensorimotor cortex. these data are indicative of cerebral hypoxia during embryogenesis. the injection of synthetic opioid peptide dalargin during critical periods of development (at the end of embryogenesis and early ontogeny) prevented ...19883416055
quantification and characterization of enkephalins in the upper part of the cat digestive tract and the coeliac ganglia.the [met]enkephalin, [leu]enkephalin and [met]enkephalin-arg-gly-leu contents of the upper part of the digestive tract (lower oesophageal sphincter, fundus, antrum, pylorus, duodenum, ileum) and coeliac ganglia of the cat were determined and identified. the enkephalin content of all the structures studied, expressed in femtomole/mg of wet tissue, was found to range from 83 to 446 with [met]enkephalin; 19 to 63 with [leu]enkephalin; 2.5 to 13 with [met]enkephalin-arg-gly-leu. in the muscular and ...19883419585
a hyperthermic syndrome in two subjects with acute hydrocephalus.although intracranial hypertension may cause autonomic disturbances, as well as alterations in the regulation of body temperature, an acute hyperthermic syndrome with autonomic disturbance as a consequence of hydrocephalus has not been described previously. two subjects presented with such a syndrome, with each of several episodes of acute shunt failure and hydrocephalus. with correction of the hydrocephalus, the autonomic disturbances and fever immediately cleared. observations from human and e ...19883415522
[sensitivity to an amoxicillin-clavulanic acid combination, of deep tonsillar flora isolated in chronic tonsillitis].deep tonsillar flora were identified in tonsils removed from 48 patients, children and adults, with chronic tonsillitis and recurrent sore throats or obstructive hypertrophy. most specimens (38/48) cultured multiple germs (2 to 5 different species) with aerobic and anaerobic gram+ and gram-forms, some being beta-lactamase producers. of the total of 135 strains isolated, 104 were aerobic and 31 anaerobic. the species of aerobic germs most frequently isolated were: apart from alpha-hemolytic strep ...19883369805
[information processing for nursing. 4. nursing information and nursing records]. 1988259818
[operating assistant or operating room assistant (tvz, 17 july 1979)]. 1988259381
[the district nurse in the diabetes home care service in the province of limburg]. 1988259376
australian nursing. 1989259844
divorce and its effects on children.in any given case, the developmental stage of the child, the parental attitude, the financial situation, the custody battle, the visitation policies, the interactions between parents prior to and after divorce and the openness with which the subject is approached will interact to produce some effect, positive or negative, on the child. the question is, as professionals, what can we do to reduce the negative effects for this population at risk? it is comforting to note that the suffering caused b ...1989260790
breastfeeding protocol. 1989260789
hemorrhage due to retrograde prolapse of stomach. an endoscopic diagnosis.a case of recurrent hemorrhage due to localized gastric mucosal congestion from retrograde prolapse during severe retching is described. historically, this condition may mimic the "mallory-weiss syndrome" and endoscopically, this lesion may be mistaken for "gastritis". this rarely recognized condition may be an explanation for some cases of cryptic hematemesis.1989313151
acute guillain-barré syndrome. 19892249465
characterization of coaggregation between bacteroides gingivalis t22 and fusobacterium nucleatum t18.bacterial adherence is a key factor in the colonization of the oral ecosystem, yet little is known about the mechanisms by which the pathogen bacteroides gingivalis adheres in the periodontal environment. we examined the ability of strains of b. gingivalis to coaggregate with selected microorganisms isolated from the subgingival microbiota of the cynomolgus monkey. a strong interaction was demonstrated between strains of b. gingivalis and fusobacterium nucleatum, whereas less pronounced or no in ...19892478473
assay for sialidase using erythrocytes and peroxidase-labeled peanut lectin.a rapid and sensitive sialidase assay method based on peroxidase-labeled peanut lectin (pna) binding to desialylated erythrocyte is described. formalinized sheep erythrocytes were used both as a stable substrate for sialidase and as a target for the lectin. in the case of sialidases from vibrio cholerae and arthrobacter ureafaciens, a linear relationship was observed between the amount of peroxidase-labeled pna bound to erythrocytes and the enzyme amount. binding of the lectin to sialidase-treat ...19892688936
high-performance liquid chromatographic determination of pirprofen and five of its metabolites in human plasma without hydrolysis and in human urine before and after chemical hydrolysis.selective high-performance liquid chromatographic methods for the simultaneous determination of pirprofen and five of its metabolites either in plasma or in urine before and after chemical hydrolysis were developed. after addition of an internal standard and a buffer, the compounds were extracted from plasma using reversed-phase c18 bond-elut columns and from urine using pre-packed silica extrelut 1 columns, back-extraction into sodium hydroxide and acidification of the alkaline phase before inj ...19892613803
falciparum malaria--present day problems. an experience with 425 cases.clinical details and present day problems encountered in 425 cases of falciparum malaria (pf) are reported. 10.11% had taken chloroquine prior to reporting to us. parasitic count done in 23.05% cases lacked correlation with severity of disease. pattern of fever varied markedly but 5.4% were afebrile throughout and presented only with bodyache and malaise. apyrexial spell was noted in 5.64%. 28.70% had typical facial looks of anaemia and sallow complexion. cerebral symptoms were noted in 3.05%. o ...19892693436
chemiluminescence of peripheral polymorphonuclear leukocytes from adult periodontitis patients.polymorphonuclear leukocytes (pmn's) constitute a primary host resistance factor against infection. this study investigated the chemiluminescent (cl) response of peripheral blood pmn's isolated from human subjects with adult periodontitis. 32 subjects were categorized on the basis of age and periodontal disease status into 4 equal groups--young healthy, young diseased, old healthy and old diseased. pmn cl was stimulated using heat-killed, serum-opsonized fusobacterium nucleatum--a specific perio ...19892646331
interleukin-1 and interleukin-1 inhibitor production by human adherent cells stimulated with periodontopathic bacteria.this study examined the effect of the putative periodontopathic bacteria bacteroides gingivalis and fusobacterium nucleatum on the production of interleukin-1 (il-1) and il-1 inhibitors by human plastic-adherent mononuclear cells from normal donors. fusobacterium mortiferum was used as a non-oral, non-pathogenic control organism. unstimulated adherent cells spontaneously secreted an il-1 inhibitor, whereas stimulation with b. gingivalis induced the synthesis and secretion of il-1. with both fuso ...19892624558
outer membrane proteins as major antigens of fusobacterium nucleatum.the immunochemical reactions of rabbit polyclonal antibodies directed to different preparations of fusobacterium nucleatum i.e, whole cells, peptidoglycan associated proteins, a peptidoglycan-protein complex and a purified 40 kilodalton (kda) protein, were investigated on outer membrane preparations of fusobacterium species and a restricted number of leptotrichia buccalis after their separation on sodium dodecyl sulphate polyacrylamide gels and electrotransfer to nitrocellulose. all f. nucleatum ...19892627499
[microbiological and serological investigation of periodontal disease activity].if disease activity in periodontal disease can be determined, the therapeutic measures of periodontitis may become diverse and different from patient to patient. the purpose of the present study was to evaluate the usefulness of microbiological and serological examination of periodontopathic bacteria in detecting destructive periodontal disease activity. one hundred and forty-eight sites in 52 subjects with moderate to severe periodontitis were studied clinically. clinical parameters included pl ...19892700196
[microbial flora of periodontitis and monitoring of the effect of initial preparation by immunofluorescence microscopy].the effect of initial preparation in adult periodontitis was evaluated by changes in clinical parameters and immunofluorescence microscopic counts of periodontal disease associated bacteria. subgingival plaque samples were taken with sterilized paper points from 10 sites of 5 periodontally healthy persons and 44 sites of 23 adult periodontitis patients. twenty-one diseased sites were periodically examined after plaque control and scaling, root planing. the direct immunofluorescence technique was ...19892700361
treatment of mouse thymus cells with phosphatidylinositol-specific phospholipase c preferentially abrogates their reactivity with autoantibodies to the thy-1 antigen.thy-1 molecules have been shown to become anchored to the membrane of thymocytes and of t-cells via a phosphatidylinositol link. in the present study the intensity of immunofluorescent staining of mouse thymus cells by monoclonal xenoantibodies and alloantibodies specific for the thy-1.2 determinant was significantly reduced following exposure of the cells to phosphatidylinositol-specific phospholipase c (pi-plc). the majority of the pi-plc-treated thymus cells retained, however, some reactivity ...19892563957
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