PMID(sorted descending)
sonic extracts from a bacterium related to periapical disease activate gelatinase a and inactivate tissue inhibitor of metalloproteinases timp-1 and examine the effects of sonicated bacterial extracts (sbes) from three related to periapical disease bacteria (porphyromonas gingivalis, p. endodontalis and f. nucleatum) on the activation of matrix metalloproteinase (mmp-2) and the inactivation of tissue inhibitors of metalloproteinase (timp-1 and timp-2).200919912382
hydrogen sulfide production from cysteine and homocysteine by periodontal and oral bacteria.hydrogen sulfide is one of the predominant volatile sulfur compounds (vscs) produced by oral bacteria. this study developed and evaluated a system for detecting hydrogen sulfide production by oral bacteria.200919905954
antibiotic resistance of periodontal pathogens obtained from frequent antibiotic users.the occurrence of bacterial strains resistant to different antimicrobials is a growing problem in latin american countries. the aim was to measure the antimicrobial susceptibility of fusobacterium nucleatum, prevotella intermedia and prophyromona gingivalis, isolated from chronic periodontitis patients. twenty-five patients diagnosed with generalized- moderate or advanced- chronic periodontitis were consecutively recruited from patients attending the periodontal specialist program, javeriana uni ...200919839485
autoaggregation response of fusobacterium nucleatum.fusobacterium nucleatum is a gram-negative oral bacterial species associated with periodontal disease progression. this species is perhaps best known for its ability to adhere to a vast array of other bacteria and eukaryotic cells. numerous studies of f. nucleatum have examined various coaggregation partners and inhibitors, but it is largely unknown whether these interactions induce a particular genetic response. we tested coaggregation between f. nucleatum atcc strain 25586 and various species ...200919837836
characterization of bacterial flora in persistent apical periodontitis lesions.microorganisms are able to survive and induce persistent infection in periapical tissues. the aim of this study was to investigate the composition of the microflora of persistent apical periodontitis lesions.200919832803
progression of chronic periodontitis can be predicted by the levels of porphyromonas gingivalis and treponema denticola in subgingival plaque.chronic periodontitis is an inflammatory disease of the supporting tissues of the teeth associated with bacteria. diagnosis is achieved retrospectively by clinical observation of attachment loss. predicting disease progression would allow for targeted preventive therapy. the aim of this study was to monitor disease progression in patients on a maintenance program and determine the levels of specific bacteria in subgingival plaque samples and then examine the ability of the clinical parameters of ...200919832799
involvement of cytosolic phospholipase a2alpha in mmp-9 up-regulation.matrix metalloproteinase-9 (mmp-9) is important in the pathogenesis of periodontitis. cytosolic phospholipase a(2)alpha (cpla(2)alpha) is involved in mmp-9 up-regulation in human monocytes. we tested the hypothesis that cpla(2)alpha also regulates mmp-9 induction by fusobacterium nucleatum and by phorbol 12-myristate-13-acetate (pma) in gingival epithelial cells. while pma induced mmp-9 expression considerably, f. nucleatum did so moderately. this time-course study demonstrated that mmp-9 mrna u ...200919828892
microbial interactions influence inflammatory host cell responses.the inflammatory response plays an important role in the tissue destruction associated with periodontitis. bacterial species can regulate the inflammatory responses of host cells, triggered by pathogens. it was hypothesized that, in the field of oral microbiology/immunology, such effects of bacterial interactions on inflammatory host cell responses might also be present. in this study, the effects of beneficial, commensal, and pathogenic species on aggregatibacter actinomycetemcomitans-induced i ...200919828891
photodynamic therapy of persistent pockets in maintenance patients-a clinical study.the aim of this study was to compare the short-term performance of a session of single photodynamic therapy (pdt) and of a conventional ultrasonic debridement (ust) in persistent pockets of maintenance patients. in a prospective, randomized, controlled, single-blind clinical study, patients with chronic periodontitis with at least two persistent pockets (>4 mm) were enrolled. they were treated either with ust (n = 29) or pdt (n = 25). clinical and microbiological examinations were performed at b ...201019823880
differential ability of periodontopathic bacteria to modulate invasion of human gingival epithelial cells by porphyromonas gingivalis.periodontitis is a polymicrobial infection caused by selected gram-negative bacteria including porphyromonas gingivalis. host cell invasion by p. gingivalis has been proposed as a possible mechanism of pathogenesis in periodontitis. the aim of the present study was to assess the influence of periodontopathogens on p. gingivalis invasion of gingival epithelial cells in polymicrobial infection. p. gingivalis was tested for its ability to invade a human gingival epithelial cell line ca9-22 in co-in ...200919818393
kinetic characterization and quaternary structure of glutamate racemase from the periodontal anaerobe fusobacterium nucleatum.cofactor-independent glutamate racemases (grs) that supply the d-glutamate required for biosynthesis of the peptidoglycan that encapsulates bacterial cells are attractive targets for the development of antibacterial drugs. recombinant gr from fusobacterium nucleatum (fngr), a gram-negative anaerobe involved in periodontal disease, was overproduced, purified, and characterized. unlike most other grs, fngr is a pseudosymmetric enzyme, catalyzing the racemization of glutamate enantiomers with simil ...200919772853
an unusual autopsy case of pyogenic liver abscess caused by periodontal bacteria.pyogenic liver abscess (pla) formation is thought to originate from the transmission of infection via three major routes including the biliary tract, portal vein and hepatic artery. however, about 50% of pla cases are considered to be cryptogenic. here we report an unusual autopsy case of pla associated with periodontopathic bacterial infection. a 59-year-old female suddenly developed cardiopulmonary arrest and died. despite macroscopic and microscopic examinations, the infectious routes and sou ...200919762989
mobilization and prevalence of a fusobacterial plasmid.fusobacterium nucleatum is a gram-negative anaerobic rod found in dental plaque biofilms, and is an opportunistic pathogen implicated in periodontitis as well as a wide range of systemic abscesses and infections. genomic analyses of f. nucleatum indicate considerable genetic diversity and a prominent role for horizontal gene transfer in the evolution of the species. several plasmids isolated from f. nucleatum, including pfn1, harbor relaxase gene homologs that may function in plasmid mobilizatio ...201019761790
mutualistic biofilm communities develop with porphyromonas gingivalis and initial, early, and late colonizers of enamel.porphyromonas gingivalis is present in dental plaque as early as 4 h after tooth cleaning, but it is also associated with periodontal disease, a late-developing event in the microbial successions that characterize daily plaque development. we report here that p. gingivalis atcc 33277 is remarkable in its ability to interact with a variety of initial, early, middle, and late colonizers growing solely on saliva. integration of p. gingivalis into multispecies communities was investigated by using t ...200919749049
fusobacterium nucleatum endocarditis mimicking polymyalgia rheumatica.a case of fusobacterium nucleatum endocarditis in an 80-year-old man is reported. the patient presented with a headache and nonspecific musculoskeletal symptoms and was misdiagnosed as having polymyalgia rheumatica. the diagnosis of bacterial endocarditis was delayed because of an insidious presentation, typical in infections with low virulence micro-organisms. the musculoskeletal symptoms, unresponsive to protracted corticosteroids, completely resolved with intravenous ampicillin treatment. rhe ...200919738533
maternal periodontal disease and soluble fms-like tyrosine kinase-1 expression.this study was conducted to examine the relationship between maternal periodontal disease and plasma angiogenic factor expression of soluble fms-like tyrosine kinase (sflt)-1.200919722802
contemporary root canal irrigants are able to disrupt and eradicate single- and dual-species biofilms.clinical/microbiological studies have consistently revealed the persistence of some bacteria after conventional root canal debridement. although this was originally attributed to the complexity of the root canal anatomy and the difficulty of delivering antibacterial agents effectively, it has emerged that the biofilm encasement of bacterial cells may confer a further mechanism of resistance. the purpose of this study was to investigate the relative disruption and bactericidal effects of root can ...200919720223
the effect of lactoferrin on oral bacterial attachment.lactoferrin (lf), an iron-binding salivary glycoprotein, plays an important role in human innate defense against local mucosal infection. we hypothesized that lf interferes with initial oral bacterial attachment to surfaces by iron sequestration, so inhibiting subsequent biofilm formation. the objective was to investigate the effect of lf on the early stages of single-species and multi-species oral biofilm development.200919702956
oral bacteria induce a differential activation of human immunodeficiency virus-1 promoter in t cells, macrophages and dendritic cells.the human immunodeficiency virus (hiv) can integrate into t cells, macrophages and dendritic cells resulting in a latent infection. reports have also demonstrated that various microbial and host cell factors can trigger hiv reactivation leading to hiv recrudescence, potentially undermining highly active antiretroviral therapies.200919702954
assessment of intraradicular bacterial composition by terminal restriction fragment length polymorphism analysis.the aim of the study was to assess the bacterial community structures associated with endodontic infections using terminal restriction fragment length polymorphism (t-rflp), and to investigate the correlation of whole community profiles with the manifestation of particular clinical features.200919702949
quantification of periodontal pathogens by paper point sampling from the coronal and apical aspect of periodontal lesions by real-time pcr.the present study compared the recovery of six periodontal pathogens by paper point samples from two different aspects of periodontal lesions by quantitative real-time polymerase chain reaction (pcr). twenty patients with untreated chronic periodontitis were randomized into two groups. before subgingival instrumentation and after 10 weeks samples in group a were taken first with a paper point half length (hp) of the probing depth, then with a paper point full length (fp) at the same site. in gro ...201019693550
use of 2-d electrophoresis and esi mass spectrometry techniques to characterize fusobacterium nucleatum proteins up-regulated after oxidative stress.the genus fusobacterium belongs to the fusobacteriaceae family and is a gram-negative obligate anaerobic bacterium found in the human oral microbiota. even that fusobacterium nucleatum cannot grow under aerobic conditions, they may exhibit aerotolerance as an adaptive response which could figure as an important virulence factor, during the stages of infection, when these anaerobes are shifted to aerobic conditions. in this regard, little is known about bacterial oxidative stress adaptive respons ...201019683589
quantitative detection of periodontopathic bacteria in atherosclerotic plaques from coronary arteries.oral pathogens, including periodontopathic bacteria, are thought to be aetiological factors in the development of cardiovascular disease. in this study, the presence of aggregatibacter actinomycetemcomitans, fusobacterium nucleatum-periodonticum-simiae group, porphyromonas gingivalis, prevotella intermedia, prevotella nigrescens and tannerella forsythia in atheromatous plaques from coronary arteries was determined by real-time pcr. forty-four patients displaying cardiovascular disease were submi ...200919679682
bone microbial contamination influences autogenous grafting in sinus augmentation.the oral occurrence of putative microbial pathogens in humans has been documented in health and disease. the presence of periodontopathogens in patients with a history of periodontal disease may have a negative impact on bone regeneration. this investigation was conducted to confirm the presence of periodontal pathogens in bone particles harvested intraorally for maxillary sinus augmentation and to assess the clinical and radiographic outcomes 6 to 12 months after bone augmentation.200919656037
[preliminary study on the discrimination of putative periodontal pathogens with a metabonomics method].to evaluate the feasibility of identifying oral pathogenic bacteria by comparing the metabolic profiling of putative periodontal pathogens and try to find a convenient and rapid way to discriminate oral microorganisms.200919637485
mixed infection involving actinomyces, aggregatibacter, and fusobacterium species presenting as perispinal tumor.a representative case in which a polymicrobial infection involving fusobacterium nucleatum, actinomyces israelii and aggregatibacter (formerly actinobacillus) actinomycetemcomitans was initially diagnosed as malignancy in an edentulous patient. additional history obtained after the nature of the syndrome was elucidated revealed that he had had his two remaining teeth extracted four months prior to this episode.201019628046
change in diet and oral hygiene over an 8-week period: effects on oral health and oral biofilm.the aim of the study was to monitor changes in oral health and oral biofilm composition in vivo during an experiment simulating prehistoric lifestyle and diet and poor oral hygiene. thirteen subjects lived for a period of 8 weeks under neolithic conditions. the following clinical parameters were recorded before and after the project: gingival and plaque index (löe and silness, acta odontol scand 21:533, 1963; silness and löe, acta odontol scand 22:121-135, 1964), probing pocket depth, and bleedi ...201019626350
proteomic analysis of endodontic infections by liquid chromatography-tandem mass spectrometry.endodontic infections are very prevalent and have a polymicrobial etiology characterized by complex interrelationships between endodontic microorganisms and the host defenses. proteomic analysis of endodontic infections can provide global insights into the invasion, pathogenicity mechanisms, and multifactorial interactions existing between root canal bacteria and the host in the initiation and progression of apical periodontitis. the purpose of this study was to apply proteomic techniques such a ...200919572900
aggregatibacter actinomycetemcomitans builds mutualistic biofilm communities with fusobacterium nucleatum and veillonella species in saliva.human oral bacterial pathogens grow in attached multispecies biofilm communities. unattached cells are quickly removed by swallowing. therefore, surface attachment is essential for growth, and we investigated multispecies community interactions resulting in mutualistic growth on saliva as the sole nutritional source. we used two model systems, saliva-coated transferable solid-phase polystyrene pegs (peg biofilms) and flow cells with saliva-coated glass surfaces. fluorescent antibody staining and ...200919564387
comparison between polymerase chain reaction-based and checkerboard dna hybridization techniques for microbial assessment of subgingival plaque compare polymerase chain reaction (pcr) with subsequent reverse hybridization (micro-ident test) and checkerboard dna-dna hybridization for the identification of 13 bacterial species in subgingival plaque samples.200919563330
fusobacterium nucleatum enters normal human oral fibroblasts in vitro.fusobacterium nucleatum, a commensal opportunistic oral bacterium, is capable of invading gingival epithelial cells, but the entrance into human primary oral fibroblast cells has not been documented. this study evaluated the ability of three strains of f. nucleatum (f. nucleatum ssp. nucleatum, f. nucleatum ssp. polymorphum, and f. nucleatum ssp. vincentii) to enter gingival fibroblasts (gfs) and periodontal ligament fibroblasts (plfs).200919563299
real-time polymerase chain reaction to determine the prevalence and copy number of epstein-barr virus and cytomegalovirus dna in subgingival plaque at individual healthy and periodontal disease sites.detection of cytomegalovirus (cmv) and epstein-barr virus (ebv) in plaque from patients with periodontal disease provides support for the theory that these viruses play a role in the pathogenesis of periodontitis. this study sought to further define this relationship by determining the prevalence of these viruses at individual disease and healthy sites of patients with periodontal disease and to determine whether the presence and amount of viral dna correlate with disease severity.200919563294
a 21-year-old man with fever and abdominal pain after recent peritonsillar abscess drainage.lemierre syndrome an extremely rare and unreported disease typically results from oropharyngeal infection and/or local tissue trauma with invasion of the parapharyngeal soft tissue with bacteria. once local tissue invasion occurs, internal jugular vein septic thrombophlebitis with or without metastatic complications may occur. the etiology of lemierre syndrome is generally fusobacterium necrophorum, reported to be present in approximately 80% of the cases. the outcome of lemierre syndrome in the ...200919555636
starvation response and growth in serum of fusobacterium nucleatum, peptostreptococcus anaerobius, prevotella intermedia, and pseudoramibacter alactolyticus.the microbiota inhabiting the untreated root canal differ markedly from those found in post-treatment disease, yet there is limited information on the microbial characteristics distinguishing the different infections. we hypothesized that starvation survival is a key microbial property in species selection. this study analyzed starvation-survival behavior over 60 days of species representative of the untreated root canal infection: fusobacterium nucleatum, peptostreptococcus anaerobius, prevotel ...200919540450
bacterial colonization of enamel in situ investigated using fluorescence in situ hybridization.oral biofilms are one of the greatest challenges in dental research. the present study aimed to investigate initial bacterial colonization of enamel surfaces in situ using fluorescence in situ hybridization (fish) over a 12 h period. for this purpose, bovine enamel slabs were fixed on buccal sites of individual splints worn by six subjects for 2, 6 and 12 h to allow biofilm formation. specimens were processed for fish and evaluated with confocal laser-scanning microscopy, using probes for eubact ...200919528150
[detection of luxs gene of quorum sensing signal system in periodontal pathogen].to detect the presence and distribution of luxs gene in quorum sensing signal system in the periodontal pathogens.200919489256
systemic immune responses in pregnancy and periodontitis: relationship to pregnancy outcomes in the obstetrics and periodontal therapy (opt) study.our previous studies reported on the obstetric, periodontal, and microbiologic outcomes of women participating in the obstetrics and periodontal therapy (opt) study. this article describes the systemic antibody responses to selected periodontal bacteria in the same patients.200919485826
fetal exposure to oral pathogens and subsequent risk for neonatal intensive care admission.maternal periodontal infection has been associated with adverse maternal and neonatal outcomes. in utero fetal exposure to oral pathogens was also recognized as deleterious to the fetus. the objective of this study was to determine the relationship between fetal exposure to oral pathogens and neonatal intensive care unit (nicu) admission.200919485816
fusobacterium nucleatum, the first gram-negative bacterium demonstrated to produce polyglutamate.poly-gamma-glutamate has been described in many gram-positive organisms. when anchored to the surface, it is a capsule and as such a virulence factor. based on sequence similarities, few gram-negative organisms have been suggested to synthesize poly-gamma-glutamate. for the first time, a gram-negative bacterium, fusobacterium nucleatum, is shown to produce and secrete poly-gamma-glutamate. putative poly-gamma-glutamate-synthesizing genes from gram-negative organisms have been compared with their ...200919483793
methods for optimizing dna extraction before quantifying oral bacterial numbers by real-time pcr.methods for the optimal extraction of genomic dna for real-time pcr enumeration of oral bacteria using the muramidase, mutanolysin, were developed using a simple in vitro oral flora model comprised of the facultative anaerobic gram-positive bacteria, lactobacillus acidophilus and streptococcus mutans, the gram-positive anaerobe, parvimonas micra, and the gram-negative anaerobes, porphyromonas gingivalis, prevotella melaninogenica and fusobacterium nucleatum. traditional, as well as more elaborat ...200919459962
proteomics of porphyromonas gingivalis within a model oral microbial community.porphyromonas gingivalis is a periodontal pathogen that resides in a complex multispecies microbial biofilm community known as dental plaque. confocal laser scanning microscopy showed that p. gingivalis can assemble into communities in vitro with streptococcus gordonii and fusobacterium nucleatum, common constituents of dental plaque. whole cell quantitative proteomics, along with mutant construction and analysis, were conducted to investigate how p. gingivalis adapts to this three species commu ...200919454014
bacteria in the apical root canal of teeth with primary apical periodontitis.bacteria settled in the apical root canal are in a privileged position to inflict damage to the periradicular tissues. therefore, the species identified in this region can be of special relevance for the pathogenesis of apical periodontitis. this study investigated the occurrence and levels of several bacterial taxa in the apical root canal of teeth with apical periodontitis.200919426923
purification and partial characterization of a bacteriocin produced by an oral fusobacterium nucleatum purify and partially characterize a bacteriocin produced by a fusobacterium nucleatum strain.200919426276
mouse model of experimental periodontitis induced by porphyromonas gingivalis/fusobacterium nucleatum infection: bone loss and host compare the effect of oral infection with porphyromonas gingivalis or fusobacterium nucleatum versus infection with both bacteria on mouse periodontal tissues, and to characterize the inflammatory response.200919419440
polymicrobial infections, biofilms, and beyond. 200919419439
human cytomegalovirus and epstein-barr virus inhibit oral bacteria-induced macrophage activation and phagocytosis.periodontal disease is an inflammatory condition caused by periodontal microorganisms. viruses such as human cytomegalovirus (hcmv) and epstein-barr virus (ebv) are associated with certain types of periodontal disease, but their roles in promoting the disease are unclear. because both viruses infect human macrophages, cells which play key roles in the clearance of pathogenic bacteria, it is likely that the viruses alter the functional capacity of macrophages by inhibiting their defense mechanism ...200919416455
multi-species biofilm formation on dental materials and an adhesive patch.this laboratory study assessed the influence of surface roughness and contact time on the formation of a multi-species biofilm on dental materials (adhesive patch, composite, amalgam and enamel).200919408815
tgf-beta1 inhibits tlr-mediated odontoblast responses to oral bacteria.tgf-beta1 exerts diverse functions in tooth development and tissue repair, but its role in microbial defenses of the tooth is not well-understood. odontoblasts extending their cellular processes into the dentin are the first cells to recognize signals from tgf-beta1 and bacteria in carious dentin. this study aimed to determine the role of tgf-beta1 in modulating odontoblast responses to oral bacteria. we show that these responses depend upon the expression levels of microbial recognition recepto ...200919407153
decreased interleukin-2 responses to fusobacterium nucleatum and porphyromonas gingivalis in generalized aggressive periodontitis.compromised t-cell responses to periodontal pathogens may contribute to the pathogenesis of generalized aggressive periodontitis (gagp). in this study, we attempted to characterize t-helper cell (th1, th2, and th17) responses in patients with gagp and healthy controls upon stimulation with disease-relevant pathogens.200919405834
the impact of the stone age diet on gingival conditions in the absence of oral hygiene.the objective of this study was to assess the oral microbiota and clinical data in subjects without access to traditional oral hygiene methods and who ate a diet available in the stone age.200919405829
complementation of the fada mutation in fusobacterium nucleatum demonstrates that the surface-exposed adhesin promotes cellular invasion and placental colonization.fusobacterium nucleatum is a gram-negative oral anaerobe implicated in periodontal disease and adverse pregnancy outcome. the organism colonizes the mouse placenta, causing localized infection and inflammation. the mechanism of placental colonization has not been elucidated. previous studies identified a novel adhesin from f. nucleatum, fada, as being involved in the attachment and invasion of host cells. the fada deletion mutant f. nucleatum 12230 us1 was defective in host cell attachment and i ...200919398541
development of strain-specific pcr primers for the identification of fusobacterium nucleatum subsp. fusiforme atcc 51190(t) and subsp. vincentii atcc 49256(t).the objective of this study was to develop the strain-specific pcr primers for fusobacterium nucleatum subsp. fusiforme atcc 51190(t) and f. nucleatum subsp. vincentii atcc 49256(t) based on the nucleotide sequence of the fs17 and fv35 dna probes, respectively. the strain specificity was tested against 10 type strains of fusobacterium spp. or subsp., 21 clinical isolates of f. nucleatum from koreans, and five type strains of distinct fusobacterium species. primer sensitivity was determined by te ...201019398030
the oxyr homologue in tannerella forsythia regulates expression of oxidative stress responses and biofilm formation.tannerella forsythia is an anaerobic periodontal pathogen that encounters constant oxidative stress in the human oral cavity due to exposure to air and reactive oxidative species from coexisting dental plaque bacteria as well as leukocytes. in this study, we sought to characterize a t. forsythia orf with close similarity to bacterial oxidative stress response sensor protein oxyr. to analyse the role of this oxyr homologue, a gene deletion mutant was constructed and characterized. aerotolerance, ...200919389765
are putative periodontal pathogens reliable diagnostic markers?periodontitis is one of the most common chronic inflammatory diseases. a number of putative bacterial pathogens have been associated with the disease and are used as diagnostic markers. in the present study, we compared the prevalence of oral bacterial species in the subgingival biofilm of generalized aggressive periodontitis (gap) (n = 44) and chronic periodontitis (cp) (n = 46) patients with that of a periodontitis-resistant control group (pr) (n = 21). the control group consisted of subjects ...200919386852
evidence for pseudoknot formation of class i preq1 riboswitch aptamers.all in a knot. the smallest riboswitch forms a pseudoknot in solution. this is demonstrated for preq(1) class i aptamers by mutational analysis in combination with (1)h nmr-based structure probing. how pseudoknot formation mediates the mrna response through its expression platform is now open for investigation.200919382115
bacterial culture and dna checkerboard for the detection of internal contamination in dental implants.the aim of this in vitro study was to evaluate the bacterial leakage along the implant-abutment interface by the conventional bacterial culture and dna checkerboard hybridization method.200919374709
characterization of the complex bacterial communities colonizing biliary stents reveals a host-dependent diversity.this study provides a comprehensive survey of the spatial and temporal bacterial composition of biliary stent biofilms. the bacterial diversity, distribution and dynamics of 59 biliary and 4 pancreatic stent communities from 40 patients being treated at two different hospitals, which implant stents either simultaneously or consecutively, were characterized by single-strand conformation polymorphism (sscp) analysis. fifty-one phylotypes belonging to 5 bacterial phyla and 24 bacterial families wer ...200919360025
tlr2 sensing of f. nucleatum and s. sanguinis distinctly triggered gingival innate response.gingival tissue faces constant exposure to micro-organisms. it functions as part of the host response, an anti-microbial barrier that recognizes and discriminates between commensal and pathogenic bacteria. this study aimed to evaluate and compare the effects of cell wall extracts from different periodontal bacteria, commensals streptococcus sanguinis and fusobacterium nucleatum and the pathogen porphyromonas gingivalis, on the innate immune response of gingival keratinocytes and the role of tlr2 ...200919299164
fusobacterium nucleatum atcc 10953 requires actinomyces naeslundii atcc 43146 for growth on saliva in a three-species community that includes streptococcus oralis 34.formation of dental plaque is a developmental process involving initial and late colonizing species that form polymicrobial communities. fusobacteria are the most numerous gram-negative bacteria in dental plaque, but they become prevalent after the initial commensal colonizers, such as streptococci and actinomyces, have established communities. the unusual ability of these bacteria to coaggregate with commensals, as well as pathogenic late colonizers, has been proposed to facilitate colonization ...200919286780
an in vitro evaluation of hydrolytic enzymes as dental plaque control agents.the plaque-control potential of commercially available amylase, lipase and protease was evaluated by observing their effects on coaggregation and on bacterial viability within various plaque microcosms. a quantitative coaggregation assay indicated that protease significantly inhibited the extent of coaggregation of actinomyces naeslundii and streptococcus oralis (p <0.05) and of porphyromonas gingivalis and s. oralis. amylase significantly (p <0.05) increased the coaggregation of a. naeslundii v ...200919273645
purulent pericarditis in patient with esophageal cancer presenting with cardiac tamponade. 200919272728
does pregnancy have an impact on the subgingival microbiota?we investigated clinical and subgingival microbiologic changes during pregnancy in 20 consecutive pregnant women > or =18 years not receiving dental care.200919228092
effect of dietary omega-3 polyunsaturated fatty acids on experimental periodontitis in the mouse.periodontitis is an infective disease caused predominantly by gram-negative anerobes. the host inflammatory response to these bacteria causes alveolar bone loss, which characterizes periodontitis. omega-3 polyunsaturated fatty acids have recognized anti-inflammatory effects; their oxygenated derivatives are key mediators in reducing inflammation. in this study we tested the hypothesis that dietary supplementation with tuna fish oil rich in the n-3 polyunsaturated fatty acid, docosahexaenoic acid ...200919210341
effect of xylitol on an in vitro model of oral biofilm.the aim of the present study was to examine whether xylitol, at different concentrations, inhibits the formation of an experimental model of oral biofilm.200819178100
endodontic microorganism susceptibility by direct contact test.the aim of this study was to evaluate in vitro the duration of the antimicrobial effect of endodontic sealers by means of the direct contact test. the sealers tested were: endomethasone - septodont, endomethasone c-septodont, endion-voco, diaket-espe, pulp canal sealer-sybronendo, and ah26-dentsply detrey. the endodontopathic microorganisms (mo) confronted were: staphylococcus aureus (sa), candida albicans (ca), enterococcus faecalis (ef), prevotella intermedia (pi), porphyromonas gingivalis (pg ...200819177855
coenzyme recognition and gene regulation by a flavin mononucleotide riboswitch.the biosynthesis of several protein cofactors is subject to feedback regulation by riboswitches. flavin mononucleotide (fmn)-specific riboswitches, also known as rfn elements, direct expression of bacterial genes involved in the biosynthesis and transport of riboflavin (vitamin b(2)) and related compounds. here we present the crystal structures of the fusobacterium nucleatum riboswitch bound to fmn, riboflavin and antibiotic roseoflavin. the fmn riboswitch structure, centred on an fmn-bound six- ...200919169240
new bacterial compositions in root-filled teeth with periradicular lesions.the aim of this study was to isolate and detect microorganisms of root-filled teeth associated with periradicular lesions. specimens were sampled from patients undergoing root canal retreatment. the bacteria were characterized by morphologic and biochemical analysis and by 16s rrna gene sequencing. microorganisms were detected in 10 of 18 teeth. the majority of positive samples revealed a mixed culture of 2-8 species. in 2 teeth enterococcus faecalis was the only detected species. for the first ...200919166766
a novel vaccine targeting fusobacterium nucleatum against abscesses and abscess in a gum pocket, resulting from bacterial infection, is a common source of chronic halitosis. although antibiotics are generally prescribed for abscesses, they require multiple treatments with risks of creating resistant bacterial strains. here we develop a novel vaccine using ultraviolet-inactivated fusobacterium nucleatum (f. nucleatum), a representative oral bacterium for halitosis. a gum pocket model, established by continuous inoculation of f. nucleatum, was employed to validate ...200919162109
[bacteriological analysis of subgingival plaque in adolescents].to investigate the changes in characteristics of periodontal pathogens in subgingival plaque of patients with puberty gingivitis and its relevance with clinical symptoms.200819134350
the microbiota of acute apical the breadth of bacterial diversity in the oral cavity has been deciphered by molecular studies, several newly identified species/phylotypes have emerged as potential pathogens. we hypothesized that many of these species/phylotypes could also be involved with the etiology of endodontic abscesses. abscess aspirates from 42 persons were analyzed for the presence of 81 species/phylotypes by means of a reverse-capture checkerboard hybridization assay. associations between the most frequently detec ...200919131319
short-term clinical and microbiological evaluations of peri-implant diseases before and after mechanical anti-infective therapies.the aim of this study was to evaluate the clinical and microbiological effects of mechanical anti-infective therapies for mucositis and peri-implantitis.200919126114
periodontal pathogen levels in adolescents before, during, and after fixed orthodontic appliance therapy.this purpose of this study was to document and investigate changes in periodontal pathogen levels before, during, and after orthodontic treatment in adolescents.200919121507
phenotypic and genotypic analyses of clinical fusobacterium nucleatum and fusobacterium periodonticum isolates from the human gut.fusobacterium nucleatum is a gram-negative anaerobic rod that is part of the normal human microflora, and has also been associated with various infections. bacterial strains belonging to the species are typically heterogeneous in both phenotype and genotype, which can hinder their identification in a clinical setting. the majority of f. nucleatum isolates originate from oral sites, however the species is also a resident of the human gastrointestinal tract. the aim of this study was to compare f. ...200819114111
toll-like receptor 2 and nalp2 mediate induction of human beta-defensins by fusobacterium nucleatum in gingival epithelial cells.we previously reported that infection by fusobacterium nucleatum strongly induced the expression of both human beta-defensin 2 (hbd-2) and hbd-3 by gingival epithelial cells. the aim of this study was to characterize the pattern recognition receptors (prrs) and regulatory mechanisms involved in the induction of hbd-2 and -3 expression by f. nucleatum in gingival epithelial cells. immortalized human gingival epithelial hok-16b cells were infected with live or heat-killed f. nucleatum, and the exp ...200919103770
[detection of periodontal pathogenic bacteria dna in coronary atheromatous plaques from patients underwent coronary artery bypass graft].the aim of this study was to observe the coexistence of periodontal bacteria dna (actinobacillus actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia, tannerella. forsythensis) in coronary atheromatous plaques and subgingival plaques in patients scheduled for coronary artery bypass graft.200819099976
tinidazole inhibitory and cidal activity against anaerobic periodontal pathogens.the in vitro activity of tinidazole against anaerobic periodontal pathogens (25 prevotella buccae, 18 prevotella denticola, 10 prevotella intermedia, 6 prevotella melaninogenica, 5 prevotella oralis, 10 fusobacterium nucleatum and 8 veillonella spp.) was determined by agar dilution. mic(90) values (minimum inhibitory concentration for 90% of the organisms) were 8 microg/ml for veillonella spp., 4 microg/ml for p. intermedia, 2 microg/ml for p. buccae, 1 microg/ml for fusobacterium spp. and 0.5 m ...200919097755
proteolytic activities of oral bacteria on prommp-9 and the effect of synthetic proteinase inhibitors.tissue reactions to bacteria lead to proinflammatory reactions involving matrix metalloproteinases (mmps). synthetic protease inhibitors may offer new possibilities to regulate bacterial proteases. we investigated proteolytic activities of certain periodontal bacteria, their effects on the latent prommp-9, and the effects of synthetic mmp inhibitors and a serine protease inhibitor pefabloc. the strains studied were porphyromonas gingivalis, prevotella intermedia, peptostreptoccus micros, prevote ...200819088890
synergistic pathogenicity of porphyromonas gingivalis and fusobacterium nucleatum in the mouse subcutaneous chamber model.porphyromonas gingivalis and fusobacterium nucleatum are often coisolated from sites of infection, such as suppurative apical periodontitis. the synergistic pathogenicity of mixed infection of p. gingivalis hg 405 with f. nucleatum pk 1594 was studied in the mouse subcutaneous chamber model in groups of seven animals. the minimal dose for p. gingivalis hg 405 that was required to infect 100% of the chambers was reduced by 1,000-fold when animals were inoculated in the same chamber with 1 x 10(9) ...200919084132
enhanced attachment of porphyromonas gingivalis to human fibroblasts mediated by fusobacterium nucleatum.fusobacterium nucleatum and porphyromonas gingivalis are often co-isolated from endodontic infection sites. the present study tested the hypothesis that adhesin-mediated reactions might contribute to this phenomenon. f. nucleaum pk1594 and p. gingivalis pk1924 were selectively labeled with fluorochromes and allowed to adhere to human fibroblasts, either each strain alone or sequentially. the number of bacteria of each type adhering to individual fibroblasts was determined. sugar inhibition profi ...200919084131
characterization of coaggregation of fusobacterium nucleatum pk1594 with six porphyromonas gingivalis strains.coaggregation is a key mechanism in biofilm formation. the aim of this study was to characterize the coaggregation of fusobacterium nucleatum pk1594 with six porphyromonas gingivalis strains in terms of kinetics and sugars inhibition. this coaggregation was quantitatively characterized by using a kinetic coaggregation assay. sugar inhibition profiles were also quantitatively defined. four types of interactions among these coaggregation partners were found: (1) fast coaggregation that was substan ...200919084124
coaggregation of treponema denticola with porphyromonas gingivalis and fusobacterium nucleatum is mediated by the major outer sheath protein of treponema denticola.coagreggation of treponema denticola with either porphyromonas gingivalis or fusobacterium nucleatum was characterized and the role of the major outer sheath protein (msp) in the coaggregation process of these bacteria was evaluated. the msp of t. denticola was found to be able to bind to p. gingivalis and f. nucleatum cells and this binding could be inhibited by msp in a concentration-dependent manner. while sodium dodecyl sulfate polyacrylamide gel electrophoresis and periodic acid-schiff (pas ...200819054094
fusobacterium nucleatum enhances invasion of human gingival epithelial and aortic endothelial cells by porphyromonas gingivalis.invasion by porphyromonas gingivalis has been proposed as a possible mechanism of pathogenesis in periodontal and cardiovascular diseases. porphyromonas gingivalis have direct access to the systemic circulation and endothelium in periodontitis patients by transient bacteremia. periodontitis can be described as one of the predominant polymicrobial infections of humans. in the present study, p. gingivalis strains were tested for their ability to invade a human gingival epithelial cell line (ca9-22 ...200819049647
oral bacteria modulate invasion and induction of apoptosis in hep-2 cells by pseudomonas aeruginosa.pseudomonas aeruginosa is an important opportunistic bacterial pathogen, causing infections of the respiratory and other organ systems in susceptible hosts. p. aeruginosa infection is initiated by adhesion to and invasion of mucosal epithelial cells. the failure of host defenses to eliminate p. aeruginosa from mucosal surfaces results in p. aeruginosa proliferation, sometimes followed by overt infection and tissue destruction. there is growing evidence that associates poor oral health and respir ...200919041936
frequency, microbial interactions, and antimicrobial susceptibility of fusobacterium nucleatum and fusobacterium necrophorum isolated from primary endodontic infections.this study assessed the prevalence and microbial interactions of fusobacterium nucleatum and fusobacterium necrophorum in primary endodontic infections from a brazilian population and their antimicrobial susceptibility to some antibiotics by the e-test. one hundred ten samples from infected teeth with periapical pathologies were analyzed by culture methods. five hundred eighty individual strains were isolated; 81.4% were strict anaerobes. f. nucleatum was found in 38 root canals and was associat ...200819026872
the fusobacterium nucleatum outer membrane protein radd is an arginine-inhibitable adhesin required for inter-species adherence and the structured architecture of multispecies biofilm.a defining characteristic of the suspected periodontal pathogen fusobacterium nucleatum is its ability to adhere to a plethora of oral bacteria. this distinguishing feature is suggested to play an important role in oral biofilm formation and pathogenesis, with fusobacteria proposed to serve as central 'bridging organisms' in the architecture of the oral biofilm bringing together species which would not interact otherwise. previous studies indicate that these bacterial interactions are mediated b ...200919007407
naturally occurring phenolic antibacterial compounds show effectiveness against oral bacteria by a quantitative structure-activity relationship study.natural and synthetic phenolic compounds were evaluated against oral bacteria. a quantitative structure-active relationship approach was applied to the germ-kill activity for a range of phenolic compounds. the lipophilicity and steric effects were found to be two key factors in determining germ-kill activity. the optimum lipophilicity, measured by the logarithm of the octanol/water partition coefficient, or log p, was found to be 5.5 for fusobacterium nucleatum , a gram-negative type of oral bac ...200819007234
crystal structure of fada adhesin from fusobacterium nucleatum reveals a novel oligomerization motif, the leucine chain.many bacterial appendages have filamentous structures, often composed of repeating monomers assembled in a head-to-tail manner. the mechanisms of such linkages vary. we report here a novel protein oligomerization motif identified in the fada adhesin from the gram-negative bacterium fusobacterium nucleatum. the 2.0 angstroms crystal structure of the secreted form of fada (mfada) reveals two antiparallel alpha-helices connected by an intervening 8-residue hairpin loop. leucine-leucine contacts pla ...200918996848
factors affecting human supragingival biofilm composition. i. plaque mass.little is known about the factors that affect the microbial composition of supragingival biofilms. this study was designed to examine the relationship between total dna probe counts of supragingival biofilm samples, clinical parameters and supragingival biofilm composition.200918973540
uncultivated bacteria as etiologic agents of intra-amniotic inflammation leading to preterm birth.intra-amniotic infection and inflammation are major causes of preterm birth (ptb). however, intra-amniotic inflammation is often detected in the absence of infection. this may partly be due to the culturing methods employed in hospital laboratories, which are unable to detect the uncultivated species. in this study, intra-amniotic microbial infections associated with ptb were examined by both culture and 16s rrna-based culture-independent methods and were corroborated by the presence of intra-am ...200918971361
participation of glutathione in the elimination of porphyromonas gingivalis in vivo.glutathione is involved in immune responses such as cell proliferation and bactericidal activity. the aim of this study was to see whether glutathione influences the intraperitoneal elimination of porphyromonas gingivalis in fusobacterium nucleatum-immunized mice.200818954348
streptococcus cristatus attenuates fusobacterium nucleatum-induced interleukin-8 expression in oral epithelial cells.oral epithelial cells may be invaded by a polymicrobial intracellular flora, including pathogens together with commensals. various oral pathogens can induce the production of interleukin-8, a potent neutrophil chemotractant, in oral epithelial cells. evidence from the gut suggests that commensal species may modulate inflammatory responses to pathogens. the aim of this study was to examine the interleukin-8 responses of oral epithelial cells to an oral pro-inflammatory species, fusobacterium nucl ...200818942189
dark field studies on oral spirochetes and fusobacterium plauti-vincenti before and after oral calcium penicillin medication. 194818875169
comparison of culture methods and multiplex pcr for the detection of periodontopathogenic bacteria in biofilm associated with severe forms of periodontitis.conventional culture methods and multiplex pcr, both of which we have been used for a long time in our clinical microbiology laboratory, were compared for their ability to detect a selected panel of periodontopathic bacteria: aggregatibacter actinomycetemcomitans, campylobacter rectus, eikenella corrodens, fusobacterium nucleatum, porphyromonas gingivalis, and prevotella intermedia. tests were performed in a single subgingival sample taken from a periodontal diseased site with a probing depth eq ...200818843894
granulocyte chemotactic protein 2 (gcp-2/cxcl6) complements interleukin-8 in periodontal disease.mucosal inflammatory responses are orchestrated largely by pro-inflammatory chemokines. the chemokine granulocyte chemotactic protein 2 (cxcl6) is involved in neutrophil recruitment and migration. previous studies have shown that granulocyte chemotactic protein 2 is up-regulated during mucosal inflammation (e.g. in inflammatory bowel disease), similarly to the functionally and structurally related chemokine interleukin-8. nevertheless, unlike interleukin-8, a role of granulocyte chemotactic prot ...200918842116
periodontal bacterial profiles in pregnant women: response to treatment and associations with birth outcomes in the obstetrics and periodontal therapy (opt) study.a recent clinical trial (obstetrics and periodontal therapy [opt] study) demonstrated that periodontal therapy during pregnancy improved periodontal outcomes but failed to impact preterm birth. the present study evaluated seven target bacteria, aggregatibacter actinomycetemcomitans (previously actinobacillus actinomycetemcomitans), porphyromonas gingivalis, treponema denticola, tannerella forsythia (previously t. forsythensis), prevotella intermedia, campylobacter rectus, and fusobacterium nucle ...200818834241
[multiple brain abscesses of odontogenic origin caused by fusobacterium nucleatum].infection caused by the anaerobic gram-negative rod fusobacterium nucleatum belongs to endogenous infections. a 41-year-old man developed multiple brain abscesses of odontogenic origin. the main causes were poor oral hygiene and no dental check-ups. pus aspired from a site in the brain contained dna of the bacterium fusobacterium nucleatum. several teeth with periapical abscesses were extracted. initially, the patient was treated empirically with a combination of cefotaxime and metronidazole (18 ...200818821484
olfactory perception of cysteine-s-conjugates from fruits and vegetables.volatile sulfur compounds have a low odor threshold, and their presence at microgram per kilogram levels in fruits and vegetables influences odor quality. sensory analysis demonstrates that naturally occurring, odorless cysteine- s-conjugates such as s-( r/ s)-3-(1-hexanol)- l-cysteine in wine, s-(1-propyl)- l-cysteine in onion, and s-(( r/ s)-2-heptyl)- l-cysteine in bell pepper are transformed into volatile thiols in the mouth by microflora. the time delay in smelling these volatile thiols was ...200818811169
effects of low-energy shock waves on oral bacteria.we have recently demonstrated that extracorporeal shock-wave therapy (eswt) is effective in promoting the healing of dermal wounds and in regenerating alveolar bone lost through periodontal disease. the objective of the present study was to determine any antibacterial effect of eswt on oral bacteria. monoculture suspensions of 6 bacterial species were treated with 100 to 500 pulses of eswt at energy flux densities (efd) of 0.12 mj/mm(2), 0.22 mj/mm(2), and 0.3 mj/mm(2). following treatment, aliq ...200818809745
fusobacterial infections: clinical spectrum and incidence of invasive disease.clinically significant infections caused by members of the genus fusobacterium are rare. we sought to describe the spectrum of clinical disease and epidemiology of these conditions presenting to an acute hospital over a five year period.200818805588
stimulation of epithelial cell matrix metalloproteinase (mmp-2, -9, -13) and interleukin-8 secretion by fusobacteria.bacterial pathogens involved in periodontal diseases exert their destructive effects primarily by stimulating the host cells to increase their secretion of proinflammatory cytokines and matrix metalloproteinases (mmps). this study aimed to determine the epithelial cell matrix metalloproteinase and interleukin-8 (il-8) secretion upon exposure to fusobacteria.200818793368
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