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quantification by qpcr of pathobionts in chronic periodontitis: development of predictive models of disease severity at site-specific level.currently, there is little evidence available on the development of predictive models for the diagnosis or prognosis of chronic periodontitis based on the qpcr quantification of subgingival pathobionts. our objectives were to: (1) analyze and internally validate pathobiont-based models that could be used to distinguish different periodontal conditions at site-specific level within the same patient with chronic periodontitis; (2) develop nomograms derived from predictive models. subgingival plaqu ...201728848499
the oral microbiome and adverse pregnancy outcomes.significant evidence supports an association between periodontal pathogenic bacteria and preterm birth and preeclampsia. the virulence properties assigned to specific oral pathogenic bacteria, for example, fusobacterium nucleatum, porphyromonas gingivalis, filifactor alocis, campylobacter rectus, and others, render them as potential collaborators in adverse outcomes of pregnancy. several pathways have been suggested for this association: 1) hematogenous spread (bacteremia) of periodontal pathoge ...201728848365
relatively low invasive capacity of porphyromonas gingivalis strains into human gingival fibroblasts in vitro.bacterial invasion into host cells is a common strategy to escape the host immune system. gingival fibroblasts (gfs) are the most predominant non-phagocytic cell type in gingival connective tissue. therefore, invasion into gfs was thought to be the first strategy for the survival of porphyromonas gingivalis. the present study compared the invasive ability of p. gingivalis into gfs with those of other red-complex and relatively less pathogenic bacterial strains, especially fusobacterium nucleatum ...201728841474
impaired polymorphonuclear neutrophils in the oral cavity of edentulous individuals.oral health is characterized by functional oral polymorphonuclear neutrophils (opmns). edentulism might be associated with a loss of opmns because these cells enter the oral cavity primarily through the gingival crevices. the main aim of this study was to investigate the numbers of opmns in rinse samples obtained from edentulous (n = 21) and dentate (n = 20) subjects. a second study aim was to investigate possible differences between opmns and peripheral blood polymorphonuclear neutrophils (cpmn ...201728833699
cancer associated faecal microbial markers in colorectal cancer detection.colorectal cancer (crc) is the second most common cause of cancer death in the western world. an effective screening program leading to early detection of disease would severely reduce the mortality of crc. alterations in the gut microbiota has been linked to crc, but the potential of microbial markers for use in crc screening has been largely unstudied. we used a nested case-control study of 238 study subjects to explore the use of microbial markers for clba+ bacteria harbouring the pks pathoge ...201728833079
periopathogens differ in terms of the susceptibility to toluidine blue o-mediated photodynamic inactivation.the main goal of periodontal therapy is to eliminate the infection spreading in periodontium. antimicrobial photodynamic therapy may be applied in order to eradicate pathogens remaining in periodontal tissues after conventional mechanical debridement, thus, improve the treatment results. the aim of this in vitro study was to evaluate the susceptibility of selected key periopathogens to toluidine blue o-mediated photodynamic inactivation and the influence of photosensitizer's concentration and li ...201728826733
fusobacterium nucleatum detected simultaneously in a pyogenic liver abscess and advanced sigmoid colon cancer.fusobacterium nucleatum is an invasive, adherent, and pro-inflammatory anaerobic bacterium involved in various infections and colorectal cancer. we report a case with pyogenic liver abscess, diagnosed with advanced sigmoid colon cancer, in whom f. nucleatum was simultaneously detected. in this patient, f. nucleatum was systematically analyzed using the molecular biological techniques of metagenome analysis, conventional pcr, and microbial fluorescence in situ hybridization.201728823592
fusobacterium nucleatum as a prognostic marker of colorectal cancer in a japanese population.accumulating evidence shows an overabundance of fusobacterium nucleatum in colorectal tumor tissues. however, the correlation between the absolute copy number of f. nucleatum in colorectal cancer tissues and colorectal cancer progression is unclear from previous reports. therefore, we performed a study to compare the abundance of f. nucleatum in colorectal tissues with clinicopathologic and molecular features of colorectal cancer.201728823057
planktonic growth of pseudomonas aeruginosa around a dual-species biofilm supports the growth of fusobacterium nucleatum within that biofilm.the goal of this study was to understand the potential interaction between pseudomonas aeruginosa and fusobacterium nucleatum within the middle ear.201728798773
combined antioxidant effects of neem extract, bacteria, red blood cells and lysozyme: possible relation to periodontal disease.the common usage of chewing sticks prepared from neem tree (azadirachta indica) in india suggests its potential efficacy in periodontal diseases. the objective of this study is to explore the antibacterial effects of neem leaf extract on the periodontophatic bacteria porphyromonas gingivalis and fusobacterium nucleatum, and its antioxidant capacities alone and in combination with bacteria and polycationic peptides that may be at the site of inflammation.201728797303
perturbed human sub-networks by fusobacterium nucleatum candidate virulence proteins.fusobacterium nucleatum is a gram-negative anaerobic species residing in the oral cavity and implicated in several inflammatory processes in the human body. although f. nucleatum abundance is increased in inflammatory bowel disease subjects and is prevalent in colorectal cancer patients, the causal role of the bacterium in gastrointestinal disorders and the mechanistic details of host cell functions subversion are not fully understood.201728793925
fusobacterium nucleatum promotes autophagy-mediated chemoresistance.abundant f. nucleatum is associated with disease recurrence in patients with colorectal cancer.201728778903
veillonellae catalase protects the growth of fusobacterium nucleatum in microaerophilic and streptococcus gordonii-present environments.the oral biofilm is a multispecies community in which antagonism and mutualism coexist amongst friends and foes to keep an ecological balance of community members. the pioneer colonizers such as streptococcus gordonii produce h2o2 to inhibit the growth of competitors like the mutans streptococci as well as strict anaerobic middle and later colonizers of the dental biofilm. interestingly, the veillonella species, as early colonizers, physically interact (coaggregate) with s. gordonii a putative c ...201728778894
assessment of the involvement of the macrophage migration inhibitory factor-glucocorticoid regulatory dyad in the expression of matrix metalloproteinase-2 during periodontitis.macrophage migration inhibitory factor (mif) is a proinflammatory cytokine and counter-regulator of endogenous glucocorticoids (gcs). it is implicated in acute and chronic inflammatory diseases. this study investigated the role of the mif-gc regulatory dyad in the expression and release of matrix metalloproteinase-2 (mmp-2) during periodontitis, in vivo and in vitro. in a mif-knockout (ko) mouse model of ligature-induced periodontitis, gingival tissues and blood were collected and analysed for l ...201728776753
mechanisms by which porphyromonas gingivalis evades innate immunity.the oral cavity is home to unique resident microbial communities whose interactions with host immunity are less frequently studied than those of the intestinal microbiome. we examined the stimulatory capacity and the interactions of two oral bacteria, porphyromonas gingivalis (p. gingivalis) and fusobacterium nucleatum (f. nucleatum), on dendritic cell (dc) activation, comparing them to the effects of the well-studied intestinal microbe escherichia coli (e. coli). unlike f. nucleatum and e. coli ...201728771533
involvement of nlrp10 in il-1α induction of oral epithelial cells by periodontal pathogens.this study investigated the pathogenesis of periodontitis and the role of nucleotide-binding oligomerization domain-like receptor protein 10 (nlrp10). the human oral epithelial cell line hok-16b was infected with two periodontal pathogens, tannerella forsythia and fusobacterium nucleatum, at various mois. rt-pcr and immunoblotting demonstrated that infection increased mrna and protein expression of nlrp10, respectively. the sirna-mediated nlrp10 knockdown significantly reduced il-1α expression a ...201728766990
prevalence of periodontal pathogens among italian patients with chronic periodontitis: a retrospective study on 2992 patients.the aim of the present study was to evaluate the prevalence of some periodontal pathogens in italian adults with chronic periodontitis.201728757933
do quaternary ammonium monomers induce drug resistance in cariogenic, endodontic and periodontal bacterial species?antibacterial monomers were developed to combat oral biofilm acids and caries; however, little is known on whether quaternary ammonium monomers (qams) would induce drug resistance in oral bacteria. the objective of this study was to investigate the effects of new antimicrobial monomers dimethylaminohexadecyl methacrylate (dmahdm) and dimethylaminododecyl methacrylate (dmaddm) on the induction of drug resistance in eight species of cariogenic, endodontic and periodontal bacteria for the first tim ...201728755761
fusobacterium nucleatum promotes chemoresistance to colorectal cancer by modulating autophagy.gut microbiota are linked to chronic inflammation and carcinogenesis. chemotherapy failure is the major cause of recurrence and poor prognosis in colorectal cancer patients. here, we investigated the contribution of gut microbiota to chemoresistance in patients with colorectal cancer. we found that fusobacterium (f.) nucleatum was abundant in colorectal cancer tissues in patients with recurrence post chemotherapy, and was associated with patient clinicopathological characterisitcs. furthermore, ...201728753429
drugs, bugs, and cancer: fusobacterium nucleatum promotes chemoresistance in colorectal cancer.the tumor microenvironment has recently been shown to play decisive roles in chemotherapeutic response. in this issue of cell, yu et al. add to these findings by identifying the bacterium fusobacterium nucleatum as a previously unrecognized chemoresistance mediator in colorectal cancer, thereby establishing the microbiota as a potential therapeutic target.201728753421
porphyromonas gingivalis suppresses invasion of fusobacterium nucleatum into gingival epithelial cells.invasion of periodontal pathogens into periodontal tissues is an important step that can cause tissue destruction in periodontal diseases. porphyromonas gingivalis is a keystone pathogen and its gingipains are key virulence factors. fusobacterium nucleatum is a bridge organism that mediates coadhesion of disease-causing late colonizers such as p. gingivalis and early colonizers during the development of dental biofilms. the aim of this study was to investigate how p. gingivalis, in particular it ...201728748028
a case of liver abscess and fusobacterium septicemia.fusobacterium species are well described as the causative pathogen in lemierre's syndrome, a suppurative thrombophlebitis of the jugular vein. however, they are less recognized for a unique variant of lemierre's syndrome presenting with invasive intraabdominal infection and associated portal vein thrombosis. we describe a case of fusobacterium nucleatum with hepatic abscess and septic pylephlebitis.201728736715
periodontal disease and intra-amniotic complications in women with preterm prelabor rupture of membranes.periodontal disease is frequently suggested as a possible causal factor for preterm delivery. the link between periodontal disease and preterm delivery is a possible translocation of periopathogenic bacteria to the placenta and amniotic fluid as well as a systemic response to this chronic inflammatory disease. however, there is a lack of information on whether there is an association between clinical periodontal status in women with preterm prelabor rupture of membranes (pprom) and the presence ...201728724342
functional biomarkers for chronic periodontitis and insights into the roles of prevotella nigrescens and fusobacterium nucleatum; a metatranscriptome analysis.periodontitis is the most prevalent inflammatory disease worldwide and is caused by a dysbiotic subgingival biofilm. here we used metatranscriptomics to determine the functional shift from health to periodontitis, the response of individual species to dysbiosis and to discover biomarkers.201528721234
tumor targeting by fusobacterium nucleatum: a pilot study and future perspectives.colorectal adenocarcinoma (crc) is a common tumor with high mortality rates. interestingly, crc was found to be colonized by the oral anaerobic bacteria fusobacterium nucleatum, which accelerates tumor progression and enables immune evasion. the crc-specific colonization by fusobacteria is mediated through the recognition of tumor displayed gal-galnac moieties by the fusobacterial fap2 gal-galnac lectin. here, we show high gal-galnac levels in additional adenocarcinomas including those found in ...201728713780
bactericidal effect of different anti-microbial agents on fusobacterium nucleatum biofilm.the root canal anatomy of the teeth is very complex. complete debridement of the root canals is a challenge and is very important for the success of the root canal treatment. hence, this study was done to find an effective irrigant which can be used during root canal treatment.201728706760
comparisons of gut microbiota among healthy control, patients with conventional adenoma, sessile serrated adenoma, and colorectal cancer.studies on gut microbiota regarding colorectal carcinogenesis, including sessile serrated adenoma (ssa), have been scarce. the aim of this study is to investigate the role of mucosa-associated gut microbiota in the colorectal carcinogenesis.201728698865
periodontal microbioma and rheumatoid arthritis: the role of porhyromonas gingivalis.rheumatoid arthritis is a disease, which can be described as an autoimmune response after molecular mimicry caused by infective agents. the current study aims at evaluating the correlation between rhematoid arthritis (ra) and periodontal disease (pd), with special attention to the microbioma detected in the gums. thirty-four patients with rd were recruited into the current study. among rheumatic parameters, rheumatoid factor (rf), anti-citrullinated protein antibody (ccp), hla-bdr1 and das28 wer ...201728691459
genome-based reclassification of fusobacterium nucleatum subspecies at the species level.fusobacterium nucleatum is classified as four subspecies, subsp. nucleatum, polymorphum, vincentii, and animalis, based on dna-dna hybridization (ddh) patterns, phenotypic characteristics, and/or multilocus sequence analysis (mlsa). the gold standards for classification of bacterial species are ddh and 16s ribosomal rna gene (16s rdna) sequence homology. the thresholds of ddh and 16s rdna similarity for delineation of bacterial species have been suggested to be >70 and 98.65%, respectively. aver ...201728687946
investigation of a novel predictive biomarker profile for the outcome of periodontal treatment.an ability to predict the response to conventional non-surgical treatment of a periodontal site would be advantageous. however, so far the biomarkers or tests devised to achieve this have lacked sensitivity. the aim of this study was to assess the ability of a novel combination of biomarkers to predict the outcome of treatment of patients with chronic periodontitis.201728671508
microbial analysis of root canal and periradicular lesion associated to teeth with endodontic failure.the quantification of ten microorganisms at the root ends and in the surrounding periradicular lesions was performed. thirty 3 mm samples root ends and 30 samples of the surrounding chronic periapical infection were collected during apical microsurgery. samples were triturated, and the bacterial dna was obtained. the bacterial quantification was performed by using the sybr green system. at least one microorganism was detected in all patients. in both the root end and periapical samples, fusobact ...201728666877
structure of the lps o-chain from fusobacterium nucleatum strain 12230.fusobacterium nucleatum is an anaerobic bacterium found in the human mouth where it causes periodontitis. it was also found in colorectal cancer tissues and is linked with pregnancy complications, including pre-term and still births. cell surface structures of the bacterium could be implicated in pathogenesis. here we report the following structure of the lipopolysaccharide o-chain of f. nucleatum strain 12230: -6-α-d-glc-4-β-d-glcnhbu3nhbua-3-β-d-quinac4nabu- where abu and hbu indicate (r)-3-am ...201728651243
fusobacterial liver abscess: a case report and review of the literature.fusobacteriae are facultative anaerobic gram-negative bacilli which cause a range of invasive infections, amongst which pyogenic liver abscesses are rare. we describe a case of fusobacterium nucleatum liver abscess and review the relevant literature.201728633639
effectiveness in the removal of endotoxins and microbiological profile in primary endodontic infections using 3 different instrumentation systems: a randomized clinical study.this clinical study was conducted to correlate the microbiological profile and levels of endotoxins found in primary endodontic infection with the presence of clinical features and to evaluate the removal of microorganisms and endotoxins using rotary, reciprocating, and hybrid systems for biomechanical preparation.201728606669
intratumoral fusobacterium nucleatum abundance correlates with macrophage infiltration and cdkn2a methylation in microsatellite-unstable colorectal carcinoma.fusobacterium nucleatum (fn), a specific species of gut microbiota, has been suggested to be enriched in the microsatellite instability-high (msi-h) molecular subtype of colorectal carcinomas (crcs). however, the clinicopathologic and molecular factors that interact with fn in msi-h crcs are poorly understood. in this study, 16s ribosomal rna gene dna sequence of fn was quantitatively measured by real-time polymerase chain reaction in tumor dna samples from a total of 160 surgically resected msi ...201728597080
bactericidal effects of 310 nm ultraviolet light-emitting diode irradiation on oral bacteria.ultraviolet (uv) light is used for phototherapy in dermatology, and uvb light (around 310 nm) is effective for treatment of psoriasis and atopic dermatitis. in addition, it is known that uvc light (around 265 nm) has a bactericidal effect, but little is known about the bactericidal effect of uvb light. in this study, we examined the bactericidal effects of uvb-light emitting diode (led) irradiation on oral bacteria to explore the possibility of using a 310 nm uvb-led irradiation device for treat ...201728587675
fusobacterium and colorectal cancer: causal factor or passenger? results from a large colorectal cancer screening study.colorectal cancer is a leading cause of morbidity and mortality worldwide in both men and women. the gut microbiome is increasingly recognized as having an important role in human health and disease. fusobacterium has been identified in former studies as a leading gut bacterium associated with colorectal cancer, but it is still not clear if it plays an oncogenic role. in the current study, fecal samples were collected prior to bowel preparation from participants of screening colonoscopy in the g ...201728582482
crystal structure of n-acetylmannosamine kinase from fusobacterium nucleatum.sialic acids comprise a varied group of nine-carbon amino sugars that are widely distributed among mammals and higher metazoans. some human commensals and bacterial pathogens can scavenge sialic acids from their environment and degrade them for use as a carbon and nitrogen source. the enzyme n-acetylmannosamine kinase (nank; ec 2.7.1.60) belongs to the transcriptional repressors, uncharacterized open reading frames and sugar kinases (rok) superfamily. nank catalyzes the second step of the sialic ...201728580924
biofilm-induced changes to the composite surface.composites may undergo biodegradation in the oral cavity. the objective was to investigate the effect of single- and multi-species biofilms on the surface roughness and topography of two composites.201728554609
new bicyclic brominated furanones as potent autoinducer-2 quorum-sensing inhibitors against bacterial biofilm formation.bacterial behaviors such as virulence factor secretion and biofilm formation are critical for survival, and are effectively regulated through quorum sensing, a mechanism of intra- and interspecies communication in response to changes in cell density and species complexity. many bacterial species colonize host tissues and form a defensive structure called a biofilm, which can be the basis of inflammatory diseases. periodontitis, a chronic inflammatory disease affecting the periodontium, is caused ...201728554093
detection of fusobacterium nucleatum in culture-negative brain abscess by broad-spectrum bacterial 16s rrna gene pcr.fusobacterium nucleatum is a strict anaerobic microorganism commensal to the human oropharynx and gastrointestinal tract, which causes a wide spectrum of human diseases and it is an important pathogen in abscesses.201728516038
herpes simplex 1 and periopathogen role in peri-implantitis.the objectives of this study were to compare the qualitative and quantitative profiles of herpes simplex virus type i (hsv-1) in implant surfaces between participants with peri-implantitis (pi) and healthy peri-implant tissues and to quantitatively assess the relation between hsv-1 and periopathogens inside the microbiological profile associated with pi.201728512280
antimicrobial activities of stearidonic and gamma-linolenic acids from the green seaweed enteromorpha linza against several oral pathogenic bacteria.we found that the edible green seaweed enteromorpha linza displayed potent antimicrobial activity against prevotella intermedia and porphyromonas gingivalis. to elucidate the active component of e. linza, isolation procedures were performed.201328510876
inflammatory bacteriome featuring fusobacterium nucleatum and pseudomonas aeruginosa identified in association with oral squamous cell carcinoma.studies on the possible association between bacteria and oral squamous cell carcinoma (oscc) remain inconclusive, largely due to methodological variations/limitations. the objective of this study was to characterize the species composition as well as functional potential of the bacteriome associated with oscc. dna obtained from 20 fresh oscc biopsies (cases) and 20 deep-epithelium swabs (matched control subjects) was sequenced for the v1-v3 region using illumina's 2 × 300 bp chemistry. high qual ...201728500338
microbiota analysis of biofilms on experimental abutments mimicking dental implants: an in vivo model.the microbiota colonizing dental implants has been said to be similar to the microbiome surrounding teeth. in the absence of inflammation, a biofilm with pathological bacteria can cover implant surfaces exposed to the oral cavity, due for example, to a remodeling process. the aim of the present study was to identify the microbiota surrounding exposed dental implants in patients with and without a history of periodontitis through a deep-sequencing approach.201728492362
influence of chronic azithromycin treatment on the composition of the oropharyngeal microbial community in patients with severe asthma.this study of the oropharyngeal microbiome complements the previously published azithromycin in severe asthma (azisast) clinical trial, where the use of azithromycin was assessed in subjects with exacerbation-prone severe asthma. here, we determined the composition of the oropharyngeal microbial community by means of deep sequencing of the amplified 16s rrna gene in oropharyngeal swabs from patients with exacerbation-prone severe asthma, at baseline and during and after 6 months treatment with a ...201728486933
fusobacterium nucleatum subspecies animalis influences proinflammatory cytokine expression and monocyte activation in human colorectal tumors.chronic infection and associated inflammation have long been suspected to promote human carcinogenesis. recently, certain gut bacteria, including some in the fusobacterium genus, have been implicated in playing a role in human colorectal cancer development. however, the fusobacterium species and subspecies involved and their oncogenic mechanisms remain to be determined. we sought to identify the specific fusobacterium spp. and ssp. in clinical colorectal cancer specimens by targeted sequencing o ...201728483840
characterization of non-dialyzable constituents from cranberry juice that inhibit adhesion, co-aggregation and biofilm formation by oral bacteria.an extract prepared from cranberry juice by dialysis known as nondialyzable material (ndm) has been shown previously to possess anti-adhesion activity toward microbial species including oral bacteria, uropathogenic escherichia coli and helicobacter pylori. bioassay-guided fractionation of cranberry ndm was therefore undertaken to identify the anti-adhesive constituents. an aqueous acetone-soluble fraction (ndmac) obtained from sephadex lh-20 inhibited adhesion-linked activities by oral bacteria, ...201728470309
effect of preparation method and storage period on the stability of saliva dna.saliva is an attractive source for oral microbial detection and quantification since sampling is non-invasive and rapid.201728460249
antibacterial activity and cytocompatibility of an implant coating consisting of tio2 nanotubes combined with a gl13k antimicrobial peptide.prevention of implant-associated infections at an early stage of surgery is highly desirable for the long-term efficacy of implants in dentistry and orthopedics. infection prophylaxis using conventional antibiotics is becoming less effective due to the development of bacteria resistant to multiple antibiotics. an ideal strategy to conquer bacterial infections is the local delivery of antibacterial agents. therefore, antimicrobial peptide (amp) eluting coatings on implant surfaces is a promising ...201728442908
invasive fusobacterium nucleatum activates beta-catenin signaling in colorectal cancer via a tlr4/p-pak1 cascade.the underlying mechanism of fusobacterium nucleatum (fn) in the carcinogenesis of colorectal cancer (crc) is poorly understood. here, we examined fn abundance in crc tissues, as well as β-catenin, tlr4 and pak1 protein abundance in fn positive and fn negative crcs. furthermore, we isolated a strain of fn (f01) from a crc tissue and examined whether fn (f01) infection of colon cancer cells activated β-catenin signaling via the tlr4/p-pak1/p-β-catenin s675 cascade. invasive fn was abundant in 62.2 ...201728423670
locoregional effects of microbiota in a preclinical model of colon carcinogenesis.inflammation and microbiota are critical components of intestinal tumorigenesis. to dissect how the microbiota contributes to tumor distribution, we generated germ-free (gf) apc(min/+) and apc(min/+) ;il10(-/-) mice and exposed them to specific-pathogen-free (spf) or colorectal cancer-associated bacteria. we found that colon tumorigenesis significantly correlated with inflammation in spf-housed apc(min/+) ;il10(-/-) , but not in apc(min/+) mice. in contrast, small intestinal neoplasia developmen ...201728416491
improved antimicrobial effect of ginseng extract by heat transformation.the incidence of halitosis has a prevalence of 22-50% throughout the world and is generally caused by anaerobic oral microorganisms, such as fusobacterium nucleatum, clostridium perfringens, and porphyromonas gingivalis. previous investigations on the structure-activity relationships of ginsenosides have led to contrasting results. particularly, the antibacterial activity of less polar ginsenosides against halitosis-related bacteria has not been reported.201728413322
microbiological, lipid and immunological profiles in children with gingivitis and type 1 diabetes mellitus.the aim of this study was to compare the prevalence of periodontal pathogens, systemic inflammatory mediators and lipid profiles in type 1 diabetes children (dm) with those observed in children without diabetes (ndm), both with gingivitis.201728403363
porphyromonas gingivalis antibody levels and diagnosis of coronary artery disease in hiv-positive individuals.periodontal disease has been associated with cardiovascular disease in the general population. it is unknown whether igg antibody levels for periodontal pathogens are associated with the diagnosis of coronary artery disease (cad) in hiv-positive individuals.201728397248
common polymorphisms in the ifi16 and aim2 genes are associated with periodontal disease.we investigated the single nucleotide polymorphism (snp) context of a previously identified periodontitis-associated locus and examined its association with microbial, biological and periodontal disease clinical parameters.201728387608
microbial colonization at the implant-abutment interface and its possible influence on periimplantitis: a systematic review and meta-analysis.the aim of this systematic review and meta-analysis was to evaluate the microbial colonization at the implant-abutment interfaces (iai) on bone-level implants and to identify possible association with peri-implant conditions.201728359872
inhibition of the mura enzyme in fusobacterium nucleatum by potential inhibitors identified through computational and in vitro approaches.fusobacterium nucleatum plays a key role in several diseases such as periodontitis, gingivitis, appendicitis, and inflammatory bowel disease (ibd). the development of antibiotic resistance by this bacterium demands novel therapeutic intervention. our recent study has reported udp-n-acetylglucosamine 1-carboxyvinyltransferase (mura) as one of the potential target proteins in f. nucleatum. in this study, we proposed two novel mura inhibitors through in silico screening and evaluated their mode of ...201728358152
dental plaque microbial profiles of children from khartoum, sudan, with congenital heart defects.few studies have focused on the bacterial species associated with the deterioration of the dental and gingival health of children with congenital heart defects (chd). the aims of this study were (1) to examine the dental plaque of children with chd in order to quantify bacterial load and altered bacterial composition compared with children without chd; and (2) to investigate the correlation between the level of caries and gingivitis and dental biofilm bacteria among those children. in this cross ...201728326155
tea polyphenols inhibit the growth and virulence properties of fusobacterium nucleatum.fusobacterium nucleatum plays a key role in creating the pathogenic subgingival biofilm that initiates destructive periodontitis. it is also a common resident of the human gastrointestinal tract and has been associated with inflammatory bowel disease. the aim of the present study was to investigate the effects of green and black tea extracts as well as two of their bioactive components, egcg and theaflavins, on the growth and virulence properties of f. nucleatum. the tea extracts and components ...201728322293
changes in salivary microbiota increase volatile sulfur compounds production in healthy male subjects with academic-related chronic stress.to investigate the associations among salivary bacteria, oral emanations of volatile sulfur compounds, and academic-related chronic stress in healthy male subjects.201728319129
pyogenic liver abscess due to fusobacterium nucleatum in a patient with liver hemangiomas. 201728317637
subgingival microbiota in caucasian patients with desquamative gingivitis: a cross-sectional study.the presence of epithelial desquamation, erythema, and erosions on the gingival tissue is usually described in literature as desquamative gingivitis (dg). a wide range of autoimmune/dermatological disorders can manifest as dg, although the two more common are oral lichen planus and mucous membrane pemphigoid. the aim of this study was to investigate the prevalence of 11 periodonto-pathogenic microorganisms in dg cases and to compare it with microbiologic status of individuals affected by plaque- ...201728304209
effect of gallium-arsenic laser on photosensitized periodontopathic anaerobic organisms: an in vitro study.the mainstay of periodontal therapy is mechanical removal of subgingival plaque. there is considerable interest in supplementing it with the use of antibiotics and antiseptics. many drawbacks are associated with these adjunctive pharmacological regimens such as development of resistance to antibiotics and disruption of microflora of the gastrointestinal tract. hence, alternate means of killing subgingival bacteria are clearly desirable. one such method is the use of laser.201728298816
carcinogenesis mechanisms of fusobacterium nucleatum.transformed cells of cancers may be related to stromal cells, immune cells, and some bacteria such as fusobacterium nucleatum. this review aimed to evaluate carcinogenesis mechanisms of fusobacterium spp. in the oral cavity, pancreatic and colorectal cancers. these cancers are the three of the ten most prevalence cancer in the worldwide. recent findings demonstrated that f. nucleatum could be considered as the risk factor for these cancers. the most important carcinogenesis mechanisms of f. nucl ...201728292019
brain abscess potentially resulting from odontogenic focus: report of three cases and a literature review.odontogenic foci can rarely cause intracranial infection. hematogenous spread is considered to be the most important pathophysiological mechanism of intracranial infection of odontogenic origin. to investigate the oral origin of intracranial infections, oral surgeons should understand the underlying mechanisms by which oral bacteria spread to the central nervous system. however, there have been very few reports of intracranial infection resulting from odontogenic infection.201728286386
quantitative proteomic analysis of extracellular matrix extracted from mono- and dual-species biofilms of fusobacterium nucleatum and porphyromonas gingivalis.the gram-negative bacteria fusobacterium nucleatum and porphyromonas gingivalis are members of a complex dental biofilm associated with periodontal disease. in this study, we cultured f. nucleatum and p. gingivalis as mono- and dual-species biofilms, and analyzed the protein composition of the biofilms extracellular polymeric matrix (epm) by high-resolution liquid chromatography-tandem mass spectrometry. label-free quantitative proteomic analysis was used for identification of proteins and seque ...201728285095
community analysis of dental plaque and endotracheal tube biofilms from mechanically ventilated patients.mechanically ventilated patients are at risk for developing ventilator-associated pneumonia, and it has been reported that dental plaque provides a reservoir of respiratory pathogens that may aspirate to the lungs and endotracheal tube (ett) biofilms. for the first time, metataxonomics was used to simultaneously characterize the microbiome of dental plaque, etts, and non-directed bronchial lavages (nbls) in mechanically ventilated patients to determine similarities in respective microbial commun ...201728259058
response to antiseptic agents of periodontal pathogens in in vitro biofilms on titanium and zirconium surfaces.the aim of this study was to develop in vitro biofilms on sla titanium (ti-sla) and zirconium oxide (zro2) surfaces and to evaluate the effect of antiseptic agents on the number of putative periodontal pathogenic species.201728237092
[microbial biomarkers for early cancer detection].the human microbiome - the vast amount of microbes that colonize our body - play an important role in maintaining our health. changes in microbiome composition have been linked to multiple diseases including cancer. although mechanisms and causalities of these associations still have to be uncovered, microbiome alterations across various stages of disease can be utilized for novel diagnostic and prognostic tests. research on biomarkers extracted from the gut microbiome has in particular focused ...201728235227
novel bioactive root canal sealer to inhibit endodontic multispecies biofilms with remineralizing calcium phosphate ions.the objectives of this study were to: (1) develop a bioactive endodontic sealer via dimethylaminohexadecyl methacrylate (dmahdm), 2-methacryloyloxyethyl phosphorylcholine (mpc) and nanoparticles of amorphous calcium phosphate (nacp) for the first time; and (2) evaluate inhibition of early-stage and mature multispecies endodontic biofilm, bond strength to root canal dentine, and calcium (ca) and phosphate (p) ion release.201728223198
the role of il-1 gene polymorphisms (il1a, il1b, and il1rn) as a risk factor in unsuccessful implants retaining overdentures.implant-supported overdentures are an alternative predictable rehabilitation method that has a high impact on improving the patient's quality of life. however, some biological complications may interfere with the maintenance and survival of these overdenture implants. the goal of this article was to assess the factors that affect peri-implant success, through a hypothetical prediction model for biological complications of implant overdentures.201728223139
structure of the lps o-chain from fusobacterium nucleatum strain 10953, containing sialic acid.fusobacterium nucleatum is an anaerobic bacterium found in the human mouth where it causes periodontitis. recently, it has been gaining attention as a potential causative agent for colorectal cancer and is strongly linked with pregnancy complications including pre-term and still births. little is known about virulence factors of this organism and thus we have initiated studies to examine the bacterial surface glycochemistry. consistent with a recent paper suggesting that f. nucleatum strain 1059 ...201728199859
influence of glycemic control on the levels of subgingival periodontal pathogens in patients with generalized chronic periodontitis and type 2 diabetes.this study evaluated the influence of glycemic control on the levels and frequency of subgingival periodontal pathogens in patients with type 2 diabetes mellitus (dm) and generalized chronic periodontitis (chp).201728198980
analysis of the expression of nlrp3 and aim2 in periapical lesions with apical periodontitis and microbial analysis outside the apical segment of teeth.to detect the distribution and expression levels of the nucleotide-binding oligomerization domain-like receptor protein 3 (nlrp3) and the absent in melanoma 2 (aim2) inflammasomes in periapical lesions and to analyse the possible microbial stimuli outside of teeth.201728193569
in vitro evaluation of the biological compatibility and antibacterial activity of a bone substitute material consisting of silver-doped hydroxyapatite and bio-oss(®).this study evaluated biological compatibility and antibacterial activity of a bone substitute material consisting of silver-doped hydroxyapatite (agha) and bio-oss(®) with different mixture ratios in vitro and investigated its antibacterial mechanism. agha was synthesized by a chemical precipitation method. after characterization, agha was mixed with bio-oss(®) at three ratios: 1:1, 1:2, and 1:4 by weight. then, porphyromonas gingivalis (pg) and fusobacterium nucleatum (fn) were used to test the ...201728186709
identification and characterization of a novel fusobacterium nucleatum adhesin involved in physical interaction and biofilm formation with streptococcus gordonii.to successfully colonize the oral cavity, bacteria must directly or indirectly adhere to available oral surfaces. fusobacterium nucleatum plays an important role in oral biofilm community development due to its broad adherence abilities, serving as a bridge between members of the oral biofilm that cannot directly bind to each other. in our efforts to characterize the molecular mechanisms utilized by f. nucleatum to physically bind to key members of the oral community, we investigated the involve ...201728173636
fusobacterium nucleatum bacteremia presenting with portal vein thrombosis: an abdominal lemierre syndrome? 201728163049
mining the proteome of fusobacterium nucleatum subsp. nucleatum atcc 25586 for potential therapeutics discovery: an in silico approach.the plethora of genome sequence information of bacteria in recent times has ushered in many novel strategies for antibacterial drug discovery and facilitated medical science to take up the challenge of the increasing resistance of pathogenic bacteria to current antibiotics. in this study, we adopted subtractive genomics approach to analyze the whole genome sequence of the fusobacterium nucleatum, a human oral pathogen having association with colorectal cancer. our study divulged 1,499 proteins o ...201628154519
tumor potentiating mechanisms of fusobacterium nucleatum, a multifaceted microbe. 201728143770
the structure of the lps o-chain of fusobacterium nucleatum strain 25586 containing two novel monosaccharides, 2-acetamido-2,6-dideoxy-l-altrose and a 5-acetimidoylamino-3,5,9-trideoxy-gluco-non-2-ulosonic acid.fusobacterium nucleatum is an anaerobic bacterium found in the human mouth where it causes periodontitis. recently, it has been gaining attention as a potential causative agent for colorectal cancer and is strongly linked with pregnancy complications including pre-term and still births. little is known about the virulence factors of this organism, and thus we have initiated studies to examine the bacterium's surface glycochemistry. we isolated lipopolysaccharide (lps) from f. nucleatum strain 25 ...201728135570
three-species biofilm model onto plasma-treated titanium implant surface.in this study, titanium (ti) was modified with biofunctional and novel surface by micro-arc oxidation (mao) and glow discharge plasma (gdp) and we tested the development of a three-species periodontopatogenic biofilm onto the treated commercially-pure titanium (cpti) surfaces. machined and sandblasted surfaces were used as control group. several techniques for surface characterizations and monoculture on bone tissue cells were performed. a multispecies biofilm composed of streptococcus sanguinis ...201728131960
effect of opposing implant prostheses on periodontal pathogens in dentures: a pilot study.an understanding of the presence of periodontal pathogens in denture plaque is important for the treatment of patients with edentulism. however, current data are limited and inconclusive.201728126355
association of dietary patterns with risk of colorectal cancer subtypes classified by fusobacterium nucleatum in tumor tissue.fusobacterium nucleatum appears to play a role in colorectal carcinogenesis through suppression of the hosts' immune response to tumor. evidence also suggests that diet influences intestinal f nucleatum. however, the role of f nucleatum in mediating the relationship between diet and the risk of colorectal cancer is unknown.201728125762
oral microbiome and history of smoking and colorectal cancer.the equilibrium of oral microbiome may be altered by environmental factors, including cigarette smoking. several recent studies also suggest that oral pathogens causing periodontal disease, such as fusobacterium nucleatum, are involved in pathogenesis of colorectal cancer.201628111632
antibacterial efficacy of triple-layered poly(lactic-co-glycolic acid)/nanoapatite/lauric acid guided bone regeneration membrane on periodontal bacteria.a guided bone regeneration (gbr) membrane has been extensively used in the repair and regeneration of damaged periodontal tissues. one of the main challenges of gbr restoration is bacterial colonization on the membrane, constitutes to premature membrane degradation. therefore, the purpose of this study was to investigate the antibacterial efficacy of triple-layered gbr membrane composed of poly(lactic-co-glycolic acid) (plga), nanoapatite (nap) and lauric acid (la) with two types of gram-negativ ...201728111388
the use of bacteriophages to biocontrol oral biofilms.infections induced by oral biofilms include caries, as well as periodontal, and peri-implant disease, and may influence quality of life, systemic health, and expenditure. as bacterial biofilms are highly resistant and resilient to conventional antibacterial therapy, it has been difficult to combat these infections. an innovative alternative to the biocontrol of oral biofilms could be to use bacteriophages or phages, the viruses of bacteria, which are specific, non-toxic, self-proliferating, and ...201728108235
sequential colonization of periodontal pathogens in induction of periodontal disease and atherosclerosis in ldlrnull mice.periodontal disease (pd) and atherosclerotic vascular disease (asvd) are both chronic inflammatory diseases with a polymicrobial etiology and have been epidemiologically associated. the purpose is to examine whether periodontal bacteria that infect the periodontium can also infect vascular tissues and enhance pre-existing early aortic atherosclerotic lesions in ldlrnull mice. mice were orally infected with intermediate bacterial colonizer fusobacterium nucleatum for the first 12 weeks followed b ...201728104616
novel reuterin-related compounds suppress odour by periodontopathic bacteria.halitosis is caused by volatile sulphur compounds including methyl mercaptan (ch3 sh) in the oral cavity and is a serious problem that limits interpersonal social communication. the aim of study was to evaluate the effects of reuterin-related compounds (rrcs) on halitosis-related periodontopathic bacteria in vitro.201728083982
stress hormone epinephrine (adrenaline) and norepinephrine (noradrenaline) effects on the anaerobic bacteria.microbial endocrinology is a relatively new research area that already encompasses the anaerobes. stress hormones, epinephrine and norepinephrine, can affect the growth of anaerobic bacteria such as fusobacterium nucleatum, prevotella spp., porhyromonas spp., tanerella forsythia and propionibacterium acnes and can increase virulence gene expression, iron acquisition and many virulence factors of some anaerobic species such as clostridium perfringens, porphyromonas gingivalis and brachyspira pilo ...201728077337
antimicrobial effects of mastic extract against oral and periodontal pathogens.various antimicrobial agents are widely used in the therapy of oral inflammatory diseases. however, their side effects and the appearance of drug resistance justify research on natural antimicrobial agents to target oral pathogens that are safe for the host. in the present study, antimicrobial properties of mastic extract on commensal and pathogenic oral bacteria, as well as its possible cytotoxic effect toward cells of epithelial and mesenchymal origin, were evaluated and compared with the comm ...201728067105
evaluation of bacteriological profile in the apical root segment of the patients with primary apical periodontitis.apical periodontitis usually results from bacterial accumulation and contamination occurring in the root-canal system, and extending beyond the apical foramen to involve the periapical tissues. literature has a paucity of the studies that stress on the division and analysis of the pulp canal segments. the reason for this disparity might be the technique used for collecting the samples from the pulp canals. hence, we carried out the present study to evaluate the microbial flora in the apical part ...201728050984
collateral damage: insights into bacterial mechanisms that predispose host cells to cancer.infections are estimated to contribute to 20% of all human tumours. viruses are known to induce cell transformation, but evidence has also linked bacteria, such as helicobacter pylori and salmonella enterica subsp. enterica serovar typhi, to different cancer types. in addition, chlamydia trachomatis, fusobacterium nucleatum and bacteroides fragilis are associated with the development of cancer, although a causal relationship has not yet been established. bacterial effectors such as colibactin an ...201728045107
detection of fusobacterium nucleatum in two cases of empyema and lung abscess using paromomycin-vancomycin supplemented brucella hk agar.fusobacterium nucleatum was found in patients with empyema or pulmonary abscess, using paromomycin-vancomycin brucella hk agar. in vitro examination revealed that growth of the strains differed significantly in different media. clinicians should be aware that suboptimal f. nucleatum cultivation methods may result in an underestimation of its frequency.201728034636
in vitro effect of porphyromonas gingivalis methionine gamma lyase on biofilm composition and oral inflammatory response.methanethiol (methyl mercaptan) is an important contributor to oral malodour and periodontal tissue destruction. porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum are key oral microbial species that produce methanethiol via methionine gamma lyase (mgl) activity. the aim of this study was to compare an mgl knockout strain of p. gingivalis with its wild type using a 10-species biofilm co-culture model with oral keratinocytes and its effect on biofilm composition and infla ...201628033374
selective persistence of propionibacterium species fma5 following sealing of infected dentinal matrix.the objective of the present study was to test the hypothesis that nutrient deprivation by effective isolation should inactivate causative saccharolytic bacteria occupying carious lesions. vital maxillary third molar teeth were prepared by removing only the superficial necrotic material, leaving behind infected dentinal matrix, before the cavity was sealed with glass ionomer cement (gic). before sealing, lesions were biopsied to provide reference bacterial dna for microbial analysis. after an in ...201628013413
comparative study on the characteristics of weissella cibaria cmu and probiotic strains for oral care.probiotics have been demonstrated as a new paradigm to substitute antibiotic treatment for dental caries, gingivitis, and chronic periodontitis. the present work was conducted to compare the characteristics of oral care probiotics: weissella cibaria cmu (chonnam medical university) and four commercial probiotic strains. survival rates under poor oral conditions, acid production, hydrogen peroxide production, as well as inhibition of biofilm formation, coaggregation, antibacterial activity, and i ...201627999400
rt-pcr quantification of periodontal pathogens in crack users and non-users.to compare counts of aggregatibacter actinomycetemcomitans, prevotella intermedia, porphyromonas gingivalis and fusobacterium nucleatum between crack users and non-users.201727982490
murine experimental root canal infection: cytokine expression in response to f. nucleatum and e. faecalis.the aim of this study was to evaluate the gene expression of proinflammatory (rankl, tnf-a and ifn-g) and regulatory (tgf-b and il-10) cytokines as reaction to experimental infection by mono or bi-association of fusobacterium nucleatum (atcc 10953) and enterococcus faecalis (atcc 19433). f. nucleatum and e. faecalis, either in mono- or bi-association were inoculated into the root canal system (rcs) of balb/c mice. animals were sacrificed at 10 and 20 days after infection and periapical tissues s ...201627982237
streptococcus mutans spap binds to radd of fusobacterium nucleatum ssp. polymorphum.adhesin-mediated bacterial interspecies interactions are important elements in oral biofilm formation. they often occur on a species-specific level, which could determine health or disease association of a biofilm community. among the key players involved in these processes are the ubiquitous fusobacteria that have been recognized for their ability to interact with numerous different binding partners. fusobacterial interactions with streptococcus mutans, an important oral cariogenic pathogen, ha ...201627976528
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