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survival of anaerobic bacteria during transportation. 1. experimental investigations on the effect of evacuation of atmospheric air by flushing with carbon dioxide and nitrogen.the effect of evacuation of atmospheric air during transportation on recovery of anaerobic bacteria was investigated. evacuation of atmospheric air from glass tubes by flushing with pure carbon dioxide lowered the content of oxygen to about 0.4 per cent. three b. fragilis strains and one strain of fusobacterium mortiferum and of peptostreptococcus anaerobius were investigated. bacterial recovery was determined one hour and 24 hours after evacuation of atmospheric air by pure carbon dioxide and p ...19763089
should fiberoptic bronchoscopy aspirates be cultured?the reliability of fiberoptic bronchoscopy as a method to study the bacteriology of the lower respiratory tract was tested. the procedure used was suction aspiration through the inner channel after topical anesthesia with lidocaine. to detect contamination by oropharyngeal bacteria, the aspirates were cultured in patients with no evidence of active infection, comparison was made with results of transtracheal aspiration cultures, and the aspirate was tested for the presence of an oral dye marker. ...19767173
influence of pencillinase production by strains of bacteroides melaninogenicus and bacteriodes oralis on pencillin therapy of an experimental mixed anaerobic infection in mice. 19768031
susceptibility of phosphomycin as a differential character for gram negative anaerobic bacilli. 197611203
evaluation of enrichment, storage, and age of blood agar medium in relation to its ability to support growth of anaerobic bacteria.by measuring the colony size of a variety of anaerobic bacteria isolated from clinical specimens, an evaluation was made of the benefits derived from the addition of several enrichments to blood agar medium commonly used for the growth of anaerobes. similar methods were used to study the effects of various storage conditions and age of the medium. the results were compared with those obtained on freshly prepared and enriched blood agar plates as well as commercially available blood agar plates. ...197611226
leukocyte migration inhibition by bacterial antigens in patients with periodontal disease. 197712257
activity of spectinomycin against anaerobes.the in vitro inhibitory activity of spectinomycin was tested against various anaerobic bacteria. different results were obtained with different media and with different initial ph's of the media. the highest minimum inhibitory concentrations for bacteroides fragilis (formula: see text 128 mug/ml) were obtained with the use of wilkins-chalgren agar (ph 7.2) and brucella blood agar (ph 7.0). brucella blood agar at higher ph's (7.4 and 8.0) and mueller-hinton and diagnostic sensitivity test agars p ...197718985
the hydrolysis of dextran by gram negative non-sporing anaerobic bacilli. 197724035
quantitative evaluation of three commercial blood culture media for growth of anaerobic organisms.the ability of three different commercial blood culture media--brain heart infusion broth (pfizer), thiol broth (difco), and prs-peptone broth (becton, dickinson & co.)--to support the growth of five different anaerobes is described. inocula of 100 and 1,000 colony-forming units per ml were used to evaluate potential differences in survival, lag time, growth rate, and doubling times of each anaerobe in each medium. in addition, each medium was evaluated for its ability to neutralize the antibact ...197824059
acute suppurative parotitis caused by anaerobic bacteria: report of two cases.staphylococcus aureus is the predominant pathogen in acute suppurative parotitis. although anaerobic bacteria are prevalent in the normal oral and upper respiratory tract flora and frequently are involved in oral and facial infections, they have seldom been isolated from patients with suppurative parotitis. it may be that a role for anaerobes in parotitis has not been documented because of inadequate specimen transport or anaerobic culture techniques. we describe two cases of acute suppurative p ...197832513
biological and biochemical characteristics of fusobacterium necrophorum leukocidin.the production of a leukocidal exotoxin by 18-hour dialysis cultures of fusobacterium necrophorum was detected in vitro by a cytotoxicity assay, using bovine leukocytes isolated from peripheral blood. biological characteristics were determined while maximizing the in vitro assay. biochemical characteristics of heat stability and stability to degradative enzymes were investigated and indicated that the leukocidin is protein in nature. the characteristics indicate that this leukocidin is different ...197832818
urease-producing species of intestinal anaerobes and their activities.urease activities of anaerobic bacteria that constituted predominant gut flora were examined. it was demonstrated that some strains of eubacterium aerofaciens, e. lentum, and peptostreptococcus products produced urease. they were the most numerous species in human feces. all strains of bifidobacterium infantis and some strains of bacteroides multiacidus, b. bifidum, clostridium symbiosum, fusobacterium necrophorum, f. varium, lactobacillus fermentum, peptococcus asaccharolyticus, and p. prevotii ...197936839
antibiotic susceptibility of anaerobic bacteria with special reference to bacteroides fragilis.it was shown that recent swedish clinical isolates of anaerobic bacteria are susceptible to many antibiotics by the agar dilution method with the exception of the bacteroides group versus beta-lactam antibiotics or tetracyclines. strains of b. fragilis were inhibited by 4--greater than 128 micrograms benzylpenicillin or cephalothin/ml, 1.0--64 micrograms cefoxitin/ml, 0.064--2 micrograms clindamycin or metronidazole/ml, 2--8 micrograms chloramphenicol/ml, 2--16 micrograms fusidic acid/ml and 0.0 ...197937593
anaerobic pulmonary infections.the main cause of anaerobic pulmonary infections is aspiration of saliva, upper airway secretions or gastric content. predisposing conditions include prominent dental disease, chronic upper respiratory tract infections and reduced consciousness. fusobacterium nucleatum, bacteroides melaninogenicus and anaerobic gram-positive cocci are the most frequently encountered organisms. the clinical presentations are lung abscess, lung gangrene and empyema, which all tend to take a slow and indolent cours ...197937594
the predominant cultivable microbiota of the periodontal abscess. 197938308
characterization of anaerobic gram-negative bacilli by using rapid slide tests for beta-lactamase production.a total of 175 isolates of anaerobic gram-negative bacilli were tested for beta-lactamase production by using a slide test modification of the chromogenic cephalosporin (nitrocefin, glaxo, middlesex, england) assay and the iodometric slide test. included isolates were bacteroides melaninogenicus (46), b. fragilis (78), other bacteroides isolates (21), fusobacterium (25), and other gram-negative bacilli (5). both slide tests detected 25 b. melaninogenicus isolates that were beta-lactamase produce ...197941001
rapid diagnosis of anaerobic infections by gas-liquid chromatography.it was postulated that the short chain fatty acids (scfa) produced by anaerobic bacteria might serve as microbial markers in purulent material. eighteen pus specimens from various sources were analysed by gas-liquid chromatography (glc), and the scfa detected were compared with the microorganisms isolated by conventional methods. it was found that the detection of propionic, isobutyric, butyric, or isovaleric acids by direct glc of pus specimens is strong evidence for anaerobic infection but not ...197941850
sugar inhibition of oral fusobacterium nucleatum haemagglutination and cell binding. 197944185
the role of bacteroides melaninogenicus and other anaerobes in periodontal infections.recent taxonomic and anatomical studies of dental plaque associated with periodontal health and disease have demonstrated that differences in the microbial populations in plaque may be responsible for the initiation and progression of disease. the consistent isolation of large numbers of anaerobic and capnophilic bacteria from the depths of periodontal lesions has suggested an important role for these organisms. bacteria that have been isolated include capnocytophaga (bacteroides ochraceus), oth ...197944923
virulence factors of anaerobic bacteria: summary of the symposium. 197944924
letter: activity of nitrofurantoin and nifuratel against anaerobic gram-negative bacilli. 197548653
a gram stain for smears of blood cultures, body fluids and tissues.a new gram stain method for blood cultures was adapted from a method for tissue sections. this was accomplished by comparing various modifications of the latter method on positive smears prepared from a 24-hour negative blood culture seeded with staphylococcus aureus and escherichia coli. a blind study was then conducted using the new method and hucker's method on 24-hour and 72-hour blood cultures as well as a limited number of other body fluids and tissues. of the 35 positive blood cultures de ...197662513
radioimmunoassay procedure for quantitating bacterial antibody in human sera.a ria system was developed to detect antibodies in human sera against bacteria. sonicates of escherichia coli and fusobacterium polymorphum were used as antigens to sensitize plastic-coated beads; antibodies to these antigens were detected with 125i-labeled antihuman globulin. serum antibody titers against e. coli were determined by the serial dilution method; from the results the standard curve principle was applied in determining the relative amounts of antibodies against e. coli in serum samp ...197765436
cross-reactivity of bacteroides fragilis o antigens.the cross-reactivity of o antigens prepared from bacteroides fragilis, other bacteroides species and from fusobacterium has been examined by indirect haemagglutination and inhibition of haemagglutination. fifteen of 20 b. fragilis ss. fragilis strains showed o-antigenic cross-reactivity with one or more of the test strains of b. fragilis ss. fragilis: nctc 9343, lille e 323 and sbl b55. the same applies also to 3 strains classified as b. coagulans, b. hypermegas and b. putredinis. the multispeci ...197765902
abscesses of the frontal lobe of the brain secondary to covert dental sepsis.the bacterial species found in pus aspirated from brain abscesses in two patients were typical of those found in dental sepsis. subsequently apical-root abscesses were demonstrated in the upper jaws of both patients. this evidence strongly suggests that these cerebral abscesses were secondary to dental sepsis which could have spread from the teeth to the frontal lobes by several possible antaomical pathways.197879867
[the antibacterial action on dental plaque of antibiotics used in dentistry. 2. in vitro experiments on streptococcus mutans, staphylococcus aureus and fusobacterium nucleatum (author's transl)]. 197788239
endotoxins of anaerobic gram-negative rods.endotoxic lipopolysaccharides (lps) isolated from fusobacterium are similar to those of salmonella with respect to chemical composition, o-antigenic specificity and endotoxic activity. bacteroides lps are highly atypical with respect to the chemical composition, and their endotoxic activity is low. o-antigenic specificity is present. b. fragilis lps is chemotactic for polymorphonuclear leukocytes in vitro and in vivo. the chemotactic activity is brought about by activation of the alternative com ...197988759
o-antigenic cross-reactivity in fusobacterium nucleatum.lipopolysaccharides (lps) of the same chemotype isolated from seven strains of fusobacterium nucleatum were examined by indirect haemagglutination and inhibition of haemagglutination in unabsorbed and absorbed antisera. four common major antigenic specificities were detected, and two of the lps examined contained antigenic specificities not shared with any of the other lps. in addition, a few weak cross-reactions were observed, also with some lps of other chemotypes.197994236
serologic reactions of oral gram negative anaerobic bacilli.serological reactions were performed using hyperimmune rabbit antisera and antigenic preparations of leptotrichia buccalis, fusobacterium fusiforme, and fusobacterium polymorphum. all tests indicated that there was serologic cross reactivity between the two fusobacterium species. no cross reactivity could be detected between the fusobacterium species and l. buccalis. the findings suggest that f. fusiform and f. polymorphum are similar in their immunogenicity, and that the grouping of these two o ...1979105024
azo reduction of trypan blue to a known carcinogen by a cell-free extract of a human intestinal anaerobe.the azo reductase activity of a cell-free extract of fusobacterium sp. 2 is characterized using trypan blue as a substrate. either chemical reduction of this dye with sodium hydrosulfite or reduction by the cell-free extract produces a mutagenic product, o-tolidine. the o-tolidine is mutagenic in the ames salmonella/mammalian-microsome mutagenicity test when activated by a rat liver s9 preparation.1978106267
serological study of trichloroacetic acid extracts of bacteroides fragilis.immunodiffusion techniques were used on trichloroacetic acid extracts from 10 strains of bacteroides fragilis in detecting precipitating antibodies against this species in immune rabbit sera. species and even strain specificities were observed in these precipitin reactions. multiple antigens were detected in the extracts from some strains, whereas only one precipitin band per extract developed during agar-gel diffusion tests of others. the antigen extracts were found to be both heat stable and r ...1979107193
ultrastructural localisation of lipopolysaccharide-binding sites with peroxidase-conjugated lipopolysaccharides.the localisation of lipopolysaccharide-binding sites on erythrocytes with peroxidase-coupled lps is described. lps was isolated from fusobacterium nucleatum (fus mc-8) by phenol-water extraction. the lps was coupled to horseradish peroxidase by the two-step method of avrameas and ternynck (1971). the biological and serological activities of the conjugated lps were compared with those of the native material. peroxidase could be coupled to lps without significant loss of endotoxic or serological a ...1979107240
attempt to detect bovine antibody against fusobacterium necrophorum by the agar gel double diffusion test. 1979108443
an animal model of intra-abdominal sepsis. 1979111347
pediatric osteomyelitis: iii. anaerobic microorganisms.primary osteomyelitis consequent to obligate anaerobic microorganisms represents an infrequently encountered type of infection in pediatric patients. unlike osteomyelitis caused by more common microorganisms such as staphylococcus, children with osseous lesions due to anaerobic microorganisms are frequently minimally symptomatic and rarely present the classic signs of fulminant osteomyelitis. radiographically, the lesions may mimic malignant osseous tumors. fastidious microbiologic analysis of t ...1979119602
haemagglutination inhibition and aggregation of fusobacterium nucleatum by human salivary mucinous glycoproteins. 1979120169
[aquatic myxobacteria (sporocytophaga cauliformis) and the order "myxobacterales" (author's transl)].the indicator function of aquatic myxobacteria for the purpose of evaluating drinking water quality as well as their occurrence in sewage effluent make it desirable to describe these hitherto little known organisms in more detail. to this end, a comparative investigation of anaerobic myxobacteria of the genus sphaerocytophaga, two typical representatives of the order myxobacterales (myxococcus fulvus, sporocytophage cauliformis), and a strain ov vitrepscilla (vitroescilla proteolytica) was under ...1979120650
human complement activation by lipopolysaccharides from bacteroides oralis, fusobacterium nucleatum, and veillonella parvula.the properties of different lipopolysaccharide (lps) preparations to induce c3 conversion in human serum was studied by means of crossed immunoelectrophoresis. c3 conversion by the alternative pathway was evaluated after calcium depletion, and lipid a-dependent activation was measured by means of inhibition with polymyxin b sulfate. lps from bacteroides oralis converted co mainly via the alternative pathway, whereas lps from fusobacterium nucleatum and veillonella parvula const pronounced lipid ...1979121108
elective medium for the direct count of vibrio (campylobacter) fusobacteria, bacteroides, selenomonas and veillonella in the gingival crevice flora. 1975127034
total viable count and differential count of vibrio (campylobacter) sputorum, fusobacterium nucleatum, selenomonas sputigena, bacteroides ochraceus and veillonella in the inflamed and non inflamed human gingival crevice. 1975129552
some data on establishing a specific-pathogen-free guinea pig colony.detailed procedures for the germfree delivery of guinea pigs using a "dry hysterectomy" technic were given; 320 animals were alive after hysterectomy, but there was a subsequent loss of 37% of the animals, mainly during the first and second weeks. their specific-pathogen-free maintenance, feeding, and association with an anerobic flora were described. feeding 3 times a day vs twice a day decreased mortality from 48% to 20%.1976131877
[perioral dermatitis].intensive investigations between 1969-1974 on 245 patients with perioral dermatitis are reported. results are discussed with special regard to clinical behaviour, histology, differential diagnosis and etiology of the disease. eczematous sources, bacterial and mycotic infections can be ruled out as etiological factors. therapeutical considerations are discussed.1976132411
microbial succession in subgingival plaque of man. 1976133231
predominant cultivable microbiota in periodontosis. 1977138729
the capacity of lipopolysaccharides from bacteroides, fusobacterium and veillonella to produce skin inflammation and the local and generalized shwartzman reaction in rabbits. 1977143518
the anticomplementary activity of lipopolysaccharide preparations and sonicates from a strain of fusobacterium nucleatum. 1978147329
the effect of divalent cation chelators and magnesium on activation of the alternative complement pathway by fusobacterium polymorphum (nucleatum). 1979161780
susceptibility of anaerobic bacteria to metronidazole: relative resistance of non-spore-forming gram-positive baccilli.susceptibility of 358 clinical isolates of obligate anaerobes to metronidazole was determined by an agar-dilution technique. only 66% of all isolates were inhibited by 6.25 mug/ml, whereas 30% required larger than or equal to 50 mug/ml. considerable variation in susceptibility was observed among different genera and species of bacteria. fusobacterium was most senstitive, followed by clostridium, bacteroides and peptococcus, peptostreptococcus, veillonella and acidaminococcus, and non-spore-formi ...1975163867
growth curves of anaerobic bacteria in solid media.simple pour plate and spectrophotometric techniques for the evaluation of growth curves of several anaerobic bacteria on solid media are described. three basic patterns of anaerobic growth were observed. the curves obtained were very reproducible when studied on separate occasions. the curves obtained by spectrophotometric measurement were comparable to those obtained by the pour plate method, especially when a large bacterial inoculum was used. limitations in the interpretation of the results a ...1975167660
parasitic nematode larvae as agents of transmission of pathogenic bacteria. 1976189513
anaerobic bacteria in biliary disease in elderly patients.gallbladder bile from 52 elderly subjects who had undergone biliary tract surgery was examined for the presence of bacteria. twelve patients had sterile bile, 18 specimens of bile yielded anaerobes as well as aerobes, and 22 yielded aerobic bacteria only. escherichia coli was the most commonly isolated organism (30 strains). bacteroides fragilis was the most frequently encountered anaerobic bacterium and was found in 15 patients. the klebsiella-enterobacter group was the second most commonly iso ...1977192815
glucose utilization by resting cells of fusobacterium polymorphum. 1977194556
anaerobes in human biliary tracts.during a 2-year period, 1,892 patients underwent biliary tract surgery at the mayo clinic. both aerobic and anaerobic cultures of bile were performed in 371 of these patients. sixty-nine percent of the cultures were positive, and 41% (117) of these grew anaerobes, although they were present in pure culture only twice. mixed cultures most commonly contained four different organisms (three aerobes and one anaerobe). bacteroides fragilis was the single most commonly isolated anaerobe and ranked fou ...1977200634
pyruvate, phosphate dikinase of bacteroides symbiosus. catalysis of partial reactions and formation of phosphoryl and pyrophosphoryl forms of the enzyme. 1978202599
requirement of monovalent cations for enolization of pyruvate by pyruvate, phosphate dikinase. 1978202600
septicemia and meningitis caused by fusobacterium aquatile.a case of septicemia and meningitis caused by fusobacterium aquatile is described. the insidious onset of the megningitis and occurrence of multiple cranial nerve affections were outstanding features. in spite of adequate antibiotic treatment the course was protracted, and the outcome was permanent damage to 3 of the affected cranial nerves.1978209534
[culture and establishment of new mexican strains of entamoeba histolytica].mixed and monoxenic cultures of rectal exudates from three patients with amebiasis of colon, and of feces from three asymptomatic carriers of entamoeba histolytica obtained after administration of a saline cathartic, and from a case of exudate of the perianal cutaneous lesion border produced by this parasite, were done. the culture media used were for: mixed culture, boeck and drbohlav modified culture and, for monoxenic culture, diamond's monophasic tp-s-1 medium without vitamin mixture and usi ...1978211955
comparison of fusobacterium sp. versus bacteroides sp. in the monoxenic cultivation of e. histolytica.by means of the performed studies it was established that 6 fusobacteria strains enhanced the growth of e. histolytica while 4 bacteroides strains did not possess this property.1978218400
investigation of the immune response to aerobic and anaerobic intestinal bacteria in a patient with crohn's disease.the immune response to aerobic and anaerobic intestinal bacteria in a patient with crohn's disease with an intestinal fistula was investigated with various serological techniques. two aerobic bacterial species, e. dispar and p. mirabilis, and four strict anaerobic bacterial species, b. fragilis ss. fragilis, f. varium and two different strains of c. perfringens, were isolated from fistula secretion of the patient. these strains were used as antigens for tube agglutination, passive hemagglutinati ...1979223235
occurrence of anaerobic bacteria in diseases of the dog and cat.a survey for anaerobic bacteria was conducted in 314 clinical specimens from dogs and cats. a total of 187 anaerobic isolates in pure and mixed culture were isolated from 111 of the specimens that contained anaerobic bacteria. common isolated included actinomyces (9.1%), clostridium perfringens (19.3%), other clostridium spp (11.2%), peptostreptococcus anaerobius (7.5%), bacteroides melaninogenicus (13.4%), other bacteroides spp (17.6%), and fusobacterium necrophorum (5.3%). anaerobic bacteria w ...1979224739
[etiology and immunoprophylaxy problems in sheep foot-rot (author's transl)]. 1979232438
cultivation and maintenance of sphaerophorus necrophorus. part ii: influence of ph on maximum growth and acid production in medium 156.sphaerophorus necrophorus was grown in medium 156 at 40 ph values ranging from 4.93 to 9.46. growth occurred between ph 5.30 and 9.28. the optimum ph for maximum growth was 6.84 while the optimum ph for acid production was 7.70. at ph 7.70 acid production was more than double that at ph 6.84 during a 12 hour incubation period. thus, maximum growth occurs at one ph and maximum acid producation at another. since the two optima are different, acid production should be evaluated only when: 1. an opt ...1975234285
the effect of coenzyme leakage and replacement on the growth and metabolism of two fusobacteria.suspensions in water of two species of fusobacterium leaked several coenzymes when incubated at normal growth temperatures. chromatography of filtrates from these suspensions revealed the presence of nad, nadp, fmn, tetrahydrofolic acid and, in one of the two, pyridoxal phosphate. analyses of some enzymic activities in whole organisms demonstrated deficiencies in coenzymes:glutamate dehydrogenase was virtually inactive in the absence of added nad; tryptophanase activities were diminished by wash ...1975234503
continuous monitoring of ph and eh in bacterial plaque grown on a tooth in an artificial mouth.apparatus which enables the simultaneous continuous monitoring of ph and eh of bacterial dental plaque as it develops on a tooth surface in an artificial mouth is described. details of the electrodes used, monitoring equipment, and culture conditions are given. preliminary results are given showing the eh and ph values of plaque produced in vitro to be in close agreement with readings reported for plaque in vivo. the effect of the incorporation of 1 per cent sucrose in the medium on these par ...1975234712
tryptophanase from sphaerophorus funduliformis. purification, molecular weight and subunit properties.a crystalline tryptophanase can be obtained from extracts of spaerophorus funduliformis using a heat treatment, hydroxyapatite chromatography and solubility in solutions of (nh4)2so4 as a function of ph and temperature. the purified enzyme is homogeneous by several criteria. s. funduliformis tryptophanase has a specific activity of 11.5-13.5 and requires pyridoxal 5'-phosphate for enzymatic activity. like other tryptophanases that have been studied, the s. funduliformis enzyme is a tetramer prot ...1975236027
survival of anaerobic and aerobic bacteria on cotton swabs in three transport systems.the capacity of aerobic and anaerobic bacteria to survive on cotton swabs placed into a dry gassed-out co2-filled tube (dgt), dry sterile aerobic tube (dat), and a tube containing a modified stuarts' transport medium (mst), was assessed. pseudomonas aeruginosa increased in numbers by 2 and 3 logs when stored in mst and dat, respectively. the viability of p. aeruginosa, although retarded when compared to msa and dat, was not adversely affected by the co2 environment in the dgt. the mst maintaine ...1975240870
juvenile periodontitis (periodontosis).juvenile periodontitis, characterized by early age of onset, rapid rate of periodontal tissue destruction and selective tooth involvement in the absence of commensurate aetiological factors, is recognized as a distinct clinical entity. the present paper describes the clinical findings and some immunological characteristics in a group of thirty-four patients under the age of thirty. clinical aspects studied included the influence of sex, race, family history and general health. immunological find ...1977267621
serological studies of bacteroides fragilis.using direct agglutination methods, a simple serological scheme for the classification of bacteroides fragilis is described. twenty strains of b. fragilis were selected by a process of sucessive screening from 151 strains obtained from various sources. o-antigens were prepared from the 20 strains, and used to raise antisera in rabbits. each of the 20 antisera reacted with its homologous antigen and eight antisera cross-reacted with other subspecies. these cross-reactions were successfully remove ...1977269199
microbiota of gingivitis in man.a study on the predominant cultivable microorganisms inhabiting gingival crevices affected with a chronic gingivitis was carried out using the roll tube culture technique. samples were obtained from nine individuals 25--42 years of age. gram-positive rods make up 29.1% of the isolates and included mainly actinomyces naeslundii, actinomyces israelii, and actinomyces viscosus. streptococcus mitis and streptococcus sanguis together made up 26.8% of the cultivable organisms. peptostreptococcus avera ...1978276916
predominant microbiota associated with periodontal health in the aged. 1978281484
[association of campylobacter sputorum s.s. sputorum with s. fusiformis: transfer of h2 between species]. 1977282238
[anaerobic infections of month and pharynx]. 1978284632
qualitative changes in dental plaque formation related to removable partial dentures.a study was performed on the microbial composition of dental plaque developing on fifteen abutment teeth in nine partially edentulous subjects. bacteria were counted in samples of plaque which had formed on vestibular surfaces, and on proximal surfaces facing edentulous spaces, during a period when dentures were not worn, and then for a period when dentures were being worn. it was found that the presence of removable partial dentures favoured a proliferation of spiral organisms.1979286025
the bacteriology of perimandibular space infections.the bacteriology of 21 perimandibular clised-space infections was studied prospectively using optimal bacteriological techniques to collect, transport, and process specimens. there was an average of six microbial species per specimen, including approximately four anaerobes and two aerobes. the predominant aerobes were alpha-hemolytic and non-hemolytic streptococci; the predominant anaerobes were peptostreptococci, bacteroides melaninogenicus, and fusobacterium nucleatum. these findings indicate ...1979286029
[immune response in germ-free mice. establishment of human indigenous fusobacterium in germ-free mice and the role of fusobacterium as monocontaminant on the host (author's transl)]. 1977287545
long-term effects of tetracycline on the subgingival microflora. 1979287678
susceptibility of new zealand isolates to anaerobic bacteria to tinidazole.minimum inhibitory and minimum bactericidal concentrations (mic, mbc) of tinidazole for 80 new zealand isolates of anaerobic bacteria were determined. growth of 95 percent of the isolates was inhibited by 4 micrograms/ml or less of tinidazole. mbc values were the same as, or one dilution higher than, the mic.1979290923
a study of the bacteria associated with advancing periodontitis in man.samples of apical plaque were taken by means of an anaerobic gas-flushed syringe from 21 sites in eight patients. the samples were anaerobically dispersed, diluted and plated and incubated in an atmosphere of 80% n2, 10% h2 and 10% co2 for 7-21 days. all colonies on plates containing 20-50 isolates were picked, repeatedly restreaked, characterized and identified where possible by a probabilistic computer identification program. the sites were divided into four groups on the basis of clinical fea ...1979294457
surface ultrastructure of some oral bacteria.adhesion of streptococcus sanguis, fusobacterium nucleatum and an actinomyces sp. to enamel and epon and their interspecies cohesion was studied with scanning and transmission electron microscopy. for adhesion studies enamel or epon was coated with salivary macromolecules and then cells of s. sanguis and in some experiments also with f. nucleatum or actinomyces sp. cells of s. sanguis were seen scattered over the surface of a thin "pellicle" that was heavily stained, and f. nucleatum and actinom ...1979296564
clindamycin and gentamicin for aerobic and anaerobic sepsis.thirty-eight adult patients with serious pleuropulmonary, soft-tissue, bone, and intra-abdominal infections caused by combinations of aerobic, facultative, and anaerobic bacteria were treated with parenterally given clindamycin phosphate and gentamicin sulfate and surgery when appropriate. nine had associated bacteremia. in 29, infections failed to respond to other therapeutic regimens, which included penicillins, cephalosporins, aminoglycosides, and chloramphenicol. results with clindamycin and ...1977318824
[gram-negative, rod-like, asporogenic anaerobes and diseases caused by them]. 1977320581
serum antibodies to plaque bacteria in subjects with dental caries and gingivitis.correlations were sought between indices of gingival inflammation and dental caries experience and serum antibody titers to five species of oral bacteria. the material comprised 53 young adult males. a statistically significant, negative correlation was observed between the antibody titer to a pool of veillonella strains and dental caries experience. multiple regression analyses failed to reveal significant associations between periodontal disease and serum antibody titers. however, the data sug ...1977320647
the predominant cultivable microflora of advanced periodontitis.a study on the predominant cultivable microorganisms harbored in the base of deep periodontal pockets of eight patients aged 34-48 years was carried out using the roll tube culture technique. from a total of 475 isolates, 425 (89.5%) were obligate anaerobes, 356(74.9%) were gram-negative, and 441(92.8%) were categorized as rods. bacteroides melaninogenicus and fusobacterium nucleatum constituted the majority of the isolates in seven samples, but their mutual proportions differed considerably bet ...1977320648
inhibition of leukocyte migration by the agarose plate technique. application to antigen from candida albicans and fusobacterium nucleatum.leukocyte migration inhibition by antigen from candida albicans and fusobacterium nucleatum was studied in man by means of the direct leukocyte migration agarose technique (lmat). antigens were prepared by prolonged ultrasonication and were added to the leukocyte cultures in the original as well as in the concentrated solutions. significant inhibition of migration with a 10-fold concentrated solution of candida antigen was demonstrated in subjects showing apositive intracutaneous reaction to can ...1977320806
therapy for experimental intraabdominal sepsis: comparison of four cephalosporins with clindamycin plus gentamicin.an experimental model of intraabdominal sepsis in rats was used for evaluation of treatment with four cephalosporins: cephalothin, cefazolin, cefoxitin, and cefamandole. doses were 115 mg of each of the cephalosporins/kg and, in additional experiments, 350 mg of cephalothin or cefazolin/kg. response to treatment was evaluated by mortality rate and the incidence of intraabdominal abscess. all four cephalosporins and gentamicin produced a similar reduction in mortality rates. there was a marked va ...1977321707
continuous culture of mixed oral flora on hydroxyapatite-coated glass beads.methods for initiating and perpetuating a culture of mixed oral flora on hydroxyapatite (ht)-coated glass beads are described. preliminary characterization of the resultant flora showed that species common in human dental plaque were present. the composition of the flora could be manipulated by altering cultural conditions. scanning electron micrographs demonstrated that the microbes grew in densely packed microcolonies on the surface of the ht bead. a procedure for continuous culture of coloniz ...1977322612
the culture of clinical specimens for anaerobic bacteria: a comparison of three regimens.three procedures for the anaerobic culture of clinical specimens are compared: (i) a conventional bench routine, (ii) a system whereby primary anaerobic plates were incubated for 48 h before examination, and (iii) an anaerobic chamber. it was shown that the anaerobic chamber was of no advantage over bench regimens when primary anaerobic plates were incubated for 48 h before being examined.1977323488
parallel study of two techniques for identification of gram negative anaerobic bacilli.the authors have used two different methods for the identification of 45 strains of stric anaerobic gram negative rods: a study of biochemical characters by the api 20 anaerobic kit, and system proposed by sutter and finegold (susceptibility to discs loaded with antibiotics associated with some simple biochemical characters). a good correlation has been observed between those two techniques. however, a precise diagnostic can be given only by a whole study of biochemical characters, as proposed b ...1977325953
synthetic disaccharide-protein antigens for production of specific 04 and 09 antisera for immunofluorescence diagnosis of salmonella.the synthetic disaccharides abequose 1 leads to a 3 mannose and tyvelose 1 leads to a 3 mannose, representative of salmonella o-antigen 4 and 9 respectively, were covalently linked to bovine serum albumin (bsa) . antisera from rabbits immunized with these immunogens were used in indirect immunofluorescence assay for the identification of group b (o-antigen 4) and d (o-antigen 9) salmonella. a total of 1030 enteric bacterial strains were tested, including 207 group b and 55 group d salmonella. th ...1977327249
lymphoproliferative response during resolution and recurrence of naturally occurring gingivitis.the lymphocyte blastogenic response in humans to oral bacterial antigens was investigated during resolution and recurrence of naturally occurring gingivitis. the following conclusions were drawn: 1. actinomyces and fusobacterium and the host responses they evoke, are associated with gingival inflammation. 2. the magnitude of the blastogenic response to these antigens is directly related to the severity of gingival inflammation. 3. scaling may cause a transient rise in the blastogenic response. 4 ...1977328846
fusobacterium necrophorum: its characteristics and role as an animal pathogen. 1977329831
gliding motility and actinomycin d sensitivity of fusobacterium nucleatum and other gram-negative rods.six strains of gram-negative anaerobic fusiform rods (fusobacterium and bacteroides spp.), isolated from deep subgingival locations in humans, were examined for (i) gliding motility in slide cultures, (iii) cell densities on nutrient agar surfaces, and (iii) sensitivity to actinomycin d. known gliding (fbt) and nonmotile (nm) strains of myxococcus xanthus served as controls for the gliding and cell density experiments. in the present study, three strains of gram-negative fusiforms from the oral ...1977332633
anticomplementary activity of fusobacterium polymorphum in normal and c4-deficient sources of guinea pig complement.fusobacterium polymorphum has been isolated from the gingival crevice in humans and has been implicated in the immunopathology of periodontal diseases. the presence of alternative complement pathway factors in gingival crevice material suggests the contribution of this process in the manifestation of the local host response. a complement consumption assay was used to show that the anticomplementary activity of a cell-wall preparation from f. polymorphum in guinea pig complement progressed optima ...1977332638
toxicity of endotoxin from fusobacterium necrophorum strains to chick embryos. 1978348272
failure to induce in rabbits effective immunity to a mixed infection of fusobacterium necrophorum and corynebacterium pyogenes with a combined bacterin.failure to induce in rabbits effective immunity to a mixed infection of fusobacterium necrophorum and corynebacterium pyogenes with a combined bacterin. onderstepoort journal of veterinary research, 44 (4), 253--2;6 (1977). rabbits were immunized with alum-precipitated, oil adjuvant and an untreated bacterin composed of f. necrophorum and c. pyogenes. immunized rabbits were challenged intradermally with a mixture of f. necrophorum and c. pyrogenes. immunized rabbits were challenged intradermally ...1977349444
bacteriological examination of summermastitis secretions. the demonstration of bacteroidaceae.corynebacterium pyogenes was isolated from 22, peptococcus indolicus from 22, a microaerophilic coccus from 21, bacteroides melaninogenicus subsp. melaninogenicus from 18, fusobacterium necrophorum from 15, and various unidentified anaerobes from 9 of a total of 22 summermastitis secretions examined.1978351551
the bacteriology of cavitating pulmonary infections and empysema. studies of transtracheal aspirates and pleural fluid.a prospective study of bacteriology in 14 cases of cavitating pulmonary infections and empyema is reported. bacteriologic results were based on transtracheal aspirates or pleural fluid. appropriate anaerobic bacteriologic methods were employed. anaerobic bacteria were recovered in 11 patients (79%); they were the only pathogens isolated in 6 patients. the predominant species were fusobacterium nucleatum, bacteroides melaninogenicus, bacteroides fragilis and peptostreptococcus. aerobic bacteria w ...1978352421
antagonism among the normal anaerobic bacteria of the mouse gastrointestinal tract determined by immunofluorescence.strictly anaerobic bacteroides sp., eubacterium sp., and fusobacterium sp. were isolated from the cecum of a conventional mouse. an immunofluorescent method utilizing rabbit antisera specific for each of these three strains was developed to determine their population levels in the gastrointestinal tracts of gnotobiotic mice. population levels of these anaerobes in groups of gnotobiotic mice colonized with either bacteroides, eubacterium, or fusobacterium were compared with those of gnotobiotes c ...1978354525
a review. lessons from an animal model of intra-abdominal sepsis.intra-abdominal sepsis that involves multiple aerobic and anaerobic bacteria derived from the colonic flora was studied in wistar rats to determine the relative roles of various microbial species. the rats challenged with pooled colonic contents showed a biphasic disease. initially, there was acute peritonitis, escherichia coli bacteremia, and high mortality. in rats that survived this acute peritonitis stage, intra-abdominal abscesses developed, and anaerobic bacteria were the preponderant orga ...1978354591
anaerobic infections in kenyatta national hospital: bacteriological isolations. 1978359316
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