| [effect of morphine in vitro on the oxidative phosphorylation in rat liver mitochondria]. | the rates of respiration in the presence of adp and of phosphorylation as an atp-ase activity of rat liver mitochondria was inhibited was in vitro by morphine with ki=6.5 mm. the uncoupler-stimulated respiration of the mitochondria and the activity of atp-ase and synthesis of atp in the submitochondrial particles were not altered in the presence of morphine. it is suggested that morphine inhibited the adenine nucleotide transport through the mitochondrial membrane | 1975 | 179644 |
| steroid production by quartered and capsular rat adrenal gland in response to haemorrhage. | 60 min after rapid bleeding (1.5--2.0 per cent of b. w.) both aldosterone and corticosterone production rate by quartered rat adrenals were found to be elevated. however, no difference was observed in the rate of aldosterone and corticosterone production by capsular adrenals of sham operated and hypovolaemic rats. corticosterone production rate by decapsulated adrenals was much more higher after haemorrhage than in the control group. the same alterations could be observed incubating adrenal tiss ... | 1975 | 179800 |
| peptidoglycan biosynthesis in micrococcus luteus (sodonensis): transglycosidase and phosphodiesterase activities in membrane preparations. | two enzyme activities involved in the biosynthesis of peptidoglycan in micrococcus luteus (sodonensis), a transglycosidase and a phosphodiesterase, have been demonstrated in isolated membrane preparations. the transglycosidase activity promotes the in vitro synthesis of an uncross-bridged peptidoglycan that is completely susceptible to lysozyme. this in vitro-synthesized peptidoglycan consists of 76% "soluble" and 24% "insoluble" material. the soluble peptidoglycan is primarily a single low-mole ... | 1976 | 179977 |
| phosphatidylserine synthetase mutants of escherichia coli. genetic mapping and membrane phospholipid composition. | mutants of escherichia coli k-12 defective in cdp-diglyceride:l-serine phosphatidyltransferase (phosphatidylserine synthetase) can be isolated by a rapid autoradiographic screening assay described previously (raetz, c. r. h. (1975) proc. natl. acad. sci. u. s. a. 72, 2274-2278). four organisms of this kind have now been characterized. the gene (designated pss) which is altered in these mutants is closely linked to the nadb locus near minute 49 on the e. coli chromosome. strains carrying the pss- ... | 1976 | 179992 |
| nad+-malic enzyme. regulatory properties of the enzyme from ascaris suum. | the regulatory properties of the nad-dependent malic enzyme from the mitochondria of ascaris suum have been studied. the malate saturation curve exhibits sigmoidicity and the degree of this sigmoidicity increases as the ph is increased. fumarate was the only compound tested that stimulated the enzyme activity, whereas oxalacetate was the most powerful inhibitor. activation by low levels of fumarate was found to be competitive with malate. it is proposed that this stimulation has physiological si ... | 1976 | 180002 |
| reversible inactivation of phenylalanine hydroxylase by catecholamines in cultured hepatoma cells. | phenylalanine hydroxylase in reuber h4 hepatoma cell cultures can be rapidly inactivated by the addition of epinephrine, norepinephrine, dopamine, or 3,4-dihydroxyphenylalanine, in order of decreasing effectiveness, to the culture medium. the enzyme was 50% inactivated in 1 hour by 25 mum (r)-epinephrine or 45 mum (r)-norepinephrine in the medium. high concentrations of epinephrine caused a 70% inactivation in 15 min. phenylalanine hydroxylase appears to be reversibly inactivated by epinephrine ... | 1976 | 180004 |
| high molecular weight forms of adrenocorticotropic hormone are glycoproteins. | mouse pituitary tumor cells (att-20/d-16v) were incubated in medium containing [3h] glucosamine or [3h] mannose. by analyzing immunoprecipitates of cell extracts and culture medium it was shown that [3h] glucosamine and [3h] mannose were incorporated into all three high molecular weight forms of acth; label was not incorporated into mr=4,500 acth (which is thought to be similar to the 39 amino acid polypeptide form of acth, alpha(1-39)). based on sodium dodecyl sulfate-polyacrylamide gel electro ... | 1976 | 180016 |
| testosterone-estradiol-binding globulin in patients with turner's syndrome: effects of estrogens and acute growth hormone administration. | the tebg activity of plasma from patients with turner's syndrome was measured quantitatively using polyacrylamide gel electrophoresis. human growth hormone administration did not significantly change the plasma tebg levels. however, oral replacement therapy with estrogens elevated plasma tebg within 3 days; after 9 days these levels reached a maximum of three- to four-fold greater than that observed at a time prior to therapy. | 1976 | 180042 |
| natural infections of guinea-pigs. | | 1976 | 180326 |
| [bacterial decomposition of ammonium perchlorate]. | | 1976 | 180384 |
| relationship between receptor and mammary tumour virus production after stimulation by glucocorticoid. | a short exposure of primary cultures of mouse mammary tumour cells to glucocorticoids results in at least a three-fold stimulation of mammary tumour virus (mtv) production. specific interaction of glucocorticoids with the cytoplasmic and nuclear receptors can also be demonstrated. the biological potency of various steroids to stimulate mtv is related directly to the retention of the steroid-receptor complex in the nuclei. progesterone has a high affinity for the cytoplasmic receptor, is not reta ... | 1976 | 180430 |
| effects of rems deprivation on striatal dopamine and acetylcholine in rats. | changes in the concentration of striatal dopamine (da) and acetylcholine (ach) in rats deprived of rem sleep for 10 days were compared with those obtained after a 4 day deprivation procedure. animals placed on small (7 cm dia.) islands surrounded by water were completely deprived of rem sleep but able to obtain some slow-wave sleep. concentration of striatal da was significantly increased after 4 days and 10 days of rem sleep deprivation by 73 and 133%, respectively when compared to controls. le ... | 1976 | 180546 |
| the effects of spontaneous obesity on insulin binding, glucose transport, and glucose oxidation of isolated rat adipocytes. | we have studied insulin, binding, glucose transport, and glucose oxidation, using large adipocytes isolated from older, fatter rats (greater than 12-mo-old, greater than 550 g), and smaller cells obtained from younger, leaner animals (4-5-wk-old, 120-160 g). at media glucose levels less than 5 mm, basal (absence of insulin) rates of glucose oxidation are comparable in both groups of cells. however, in the presence of insulin, the increase in glucose oxidation is much greater in the smaller cells ... | 1976 | 181402 |
| [experimental aortic stenosis, its subsequent correction and the activity of oxidative enzymes in the myocardium]. | the activity of the respiratory enzymes of myocardial mitochondria--succinatedehydrogenase and cytochromixidase--was studied in experimentally induced aortic stenosis and after its elimination in 98 mongrel dogs. the observation period of stenosis was 1, 15, 30 and 60 days, and after its elimination--1, 7, 15 and 30 days. the activity of the enzymes in question was found to grow after the production of aortic stenosis. the highest level of their enzymatic activity was observed on the 15th day af ... | 1976 | 181629 |
| viruslike particles in granulomatous angiitis of the central nervous system. | neuropathologic examination of the brain of a 67-year-old woman with a 5-month history of progressive multiple neurologic deficits showed granulomatous angiitis of the small parenchymal and leptomeningeal blood vessels of the brain and spinal cord. electron microscopy of formalin-fixed brain disclosed intranuclear viruslike particles resembling herpesvirus. although definitive proof cannot be established without further virologic tests, this previously unreported finding suggests that some cases ... | 1976 | 181693 |
| experimental myasthenia due to alpha-bungarotoxin. | neuromuscular transmission was studied electrophysiologically in rabbits intoxicated by alpha-bungarotoxin, a specific inhibitor of acetylcholine receptor. the findings consisted of a slight reduction in amplitude of single evoked muscle action potentials, a decrement in amplitude of successive evoked muscle action potentials post-tetanic potentiation and exhaustion, edrophonium reversal, and no change of muscle action potentials evoked by direct stimulation of the muscle. these were similar to ... | 1976 | 181695 |
| hyperthyroidism and myasthenia gravis with features of eaton-lambert syndrome. | in a 50-year-old man with hyperthyroidism and myasthenic weakness electrophysiologic phenomena similar to eaton-lambert syndrome were seen in classical myasthenia gravis. the orbicularis oculi showed an abnormally small muscle action potential in response to nerve stimulus and unusual facilitation of the response with activity. these signs are compatible with eaton-lambert syndrome, but the findings obtained from the adductor pollicis were typical of classical myasthenia. a possible common basis ... | 1976 | 181696 |
| [relation between urinary cyclic amp, pth and histomorphometric resorption parameter. a study of 44 human iliac crest bone biopsies]. | daily urinary cyclic amp (ampcu) has been compared to pth plasma level and to bone resorption parameters in 44 cases of metabolic bone disease. in 14 cases of primary hyperparathyroidism, ampcu was always increased likewise in 4 cases of osteomalacia with secondary hyperparathyroidism. on the whole series of cases a significant correlation has been found (1) between pth plasma level and ampcu and (2) between bone resporption surfaces and ampcu. conversely, no correlation between pth and perioste ... | 1976 | 181720 |
| [significance of changes in the oxidation-reduction processes in the pathogenesis of visceral shock]. | | 1975 | 181721 |
| age-related alteration in human heart collagen. | | 1976 | 181759 |
| in vivo fate of a behaviorally active acth 4-9 analog in rats after systemic administration. | in vivo fate of a threefold substituted acth 4-9 analog with a markedly potentiated behavioral activity, 4-met(o2), 8-d-lys, 9-phe-acth 4-9, was investigated. the radioactive labeled [7-3h-phe] acth 4-9 analog was administered iv, sc and orally in a dose of approximately 40 mug. plasma concentrations of total radioactivity and intact peptide were determined at various periods after administration in urethane anesthetized rats. oral administration was also performed with conscious animals. maxima ... | 1976 | 181766 |
| resistance of pseudomonas aeruginosa to antimicrobial drugs. | | 1975 | 181789 |
| radioimmunoassay of triiodothyronine in human serum with special reference to methodological problems. | a radioimmunoassay for measuring the level of triiodothyronine in human serum has been developed and studied with respect to interference from hormone-binding substances present in human serum. dextran-coated charcoal was used for the separation of free and antibody-bound hormone. ans (8-anilino-1-naphthalenesulfonic acid) was used to reduce the interference from hormone-binding substances in serum. extraction of the hormone by ethanol before the radioimmunoassay did not solve the problem of int ... | 1976 | 181837 |
| herpesvirus infections in renal allograft recipients. | 17 of 37 immunosuppressed renal allograft recipients developed clinical and/or serological evidence of herpes simplex virus infections, 7 of 83 developed a zoster rash, 85 of loo had evidence of cytomegalovirus infection and in 3 of 30 an active epstein-barr virus was seen. | 1975 | 181840 |
| enzootic with ectromelia symptomatology in sprague-dowley rats. | | 1976 | 181904 |
| effectiveness of orally administered bordetella pertussis vaccine in mice. | oral administration of killed bordetella pertussis organisms to mice results in increased resistance to an intracerebral infection with virulent b. pertussis cells. the rate of survival is dependent on the dose of antigen. but besides specific systemic immunity, which is persistent over a long period, also transient non-specific resistance is increased. these effects are evidently induced without penetration of bacterial substances into the circulation. | 1976 | 182602 |
| epizootiology of natural infection with bovine leukemia virus. | | 1975 | 183681 |
| experimental infection of marmoset and squirrel monkeys with attenuated hvs. | | 1975 | 183695 |
| a purification procedure for the soluble cytochrome oxidase and some other respiratory proteins from pseudomonas aeruginosa. | the production of the soluble cytochrome oxidase/nitrite reductase in the bacterium pseudomonas aeruginosa is favoured by anaerobic conditions and the presence of kno3(20g/l) in the culture medium. of three methods commonly used for the disruption of bacterial suspensions (ultrasonication, liquid-shear homogenization and glass-bead grinding), sonication proved the most efficient in releasing the pseudomonas cytochrome oxidase. a polarographic assay of pseudomonas cytochrome oxidase activity with ... | 1976 | 183750 |
| the fine structure of blood vessels in ethylnitrosourea-induced tumours of the rat nervous system: with special reference to the breakdown of the blood-brain barrier. | the fine structure of capillaries in and around ethylnitrosourea-induced tumours, gliomas and schwannomas, was examined in rats. a great variation was observed in the severity of changes: the degree of abnormality depended on the histological type and size of the tumour and on the site of the capillaries within the neoplasm. endothelial cells, basement membranes and pericytes all demonstrated changes in their fine structure. the most striking alterations occurred in the endothelial cells: lumina ... | 1976 | 183804 |
| chromosome banding studies of several transplantable hepatomas. | | 1976 | 184695 |
| properties and regulation of liver phosphorylase phosphatase. | | 1976 | 184701 |
| haemorrhagic and inflammatory properties of collagenase from c. histolyticum. | collagenase from clostridium histolyticum induced haemorrhages when applied to the surface of dog lung; it exerted a similar effect on mouse lung when injected intrathoracically. injected into rat paws, bacterial collagenase induced haemorrhage and oedema. effects of collagenase were prevented by several procedures that inhibit collagenolytic activity (heating at various temperatures and incubation with metal-complexing agents such as edta, penicillamine and dithiothreitol). protein protease inh ... | 1976 | 184702 |
| increased collagen synthesis and the kinetic characteristics of prolyl hydroxylase in tissues of rabbits with experimental arteriosclerosis. | increased aortic and liver prolyl hydroxylase activity has been suggested as an early biochemical indicator of the fibrotic changes which occur in rabbits with injury induced arteriosclerosis. daily administration of epinephrine (0.025-0.050 mg/kg, i.v.) and thyroxine (0.050 mg/kg, i.p.) to rabbits for 3 weeks produced aortic fibrous plaques with a 4-fold increase in aortic prolyl hydroxylase and also a 5-fold increase in liver prolyl hydroxylase. histopathologically, the livers of these rabbits ... | 1976 | 184800 |
| clearing factor lipase (lipoprotein lipase) activator. a method for the measurement of the net activating ability of human sera. | the hydrolysis of an emulsified triglyceride substrate by clearing factor lipase (lipoprotein lipase) normally requires the presence of particular activating polypeptide species. these are present in serum, together with other inhibitory species, as part of the serum lipoproteins. the paper describes a method whereby the net activating ability of individual human sera may be measured routinely. in a normal population, this activating ability is shown to be correlated positively with the fasting ... | 1976 | 184802 |
| a relative deficiency of cytochrome p-450 and aryl hydrocarbon [benzo(a)pyrene] hydroxylase in hyperplastic nodules induced by 2-acetylaminofluorene in rat liver. | the concentrations of cytochrome p-450 and the activities of aryl hydrocarbon [benzo(a)pyrene] hydroxylase (ahh) and reduced nicotinamide adenine dinucleotide phosphate-cytochrome c reductase were measured in early (gray-white) and remodeled (brown) hyperplastic nodules induced in the livers of rats with 2-acetylaminofluorene and were compared to the values in control livers and in the liver surrounding the nodules. cytochrome p-450 content of early (14 weeks) hyperplastic nodules is 30% of the ... | 1976 | 184917 |
| localization of the human uridine monophosphate kinase gene to the p21 leads to pter region of chromosome 1. | | 1976 | 185017 |
| normal rabbit alveolar macrophages. ii. their primary and secondary lysosomes as revealed by electron microscopy and cytochemistry. | in this investigation, vacuoles containing tubular myelin proved to be digestive compartments with cytochemical reactivity for acid phosphatase and arylsulfatase. these cytochemical markers identify the secondary lysosomes, known to contain enzymes capable of hydrolyzing phospholipids like surfactant. therefore, it appears that alveolar macrophages possess the enzymatic machinery for the degradation of the tubular myelin found in their digestive vacuoles. although it thus appears evident that al ... | 1976 | 185318 |
| analysis of the rna species isolated from defective particles of vesicular stomatitis virus. | serial high multiplicity passage of a cloned stock of vesicular stomatitis virus was found to generate defective interfering particles containing three size classes of rna, with sedimentaiton coefficients of 31 s, 23 s and 19 s. the 31 s and 23 s rna species were found to be complementary to both the 12 to 18 s and 31 s size classes of vsv mrnas. the 19 s class of rna was found to be partially base-paired. all three rna species were found to contain ppap at their 5' termini. | 1976 | 185328 |
| polyoma genome in hamster bhk-21-c13 cells: integration into cellular dna and induction of the viral replication. | when grown at 39.5 degrees c, bhk-21 c-13 cells transformed by a gene mutants of polyoma virus contain viral sequences that are predominantly associated with cellular dna pelleted in the hirt lysis procedure. at this temperature, in cells that are inducible for viral dna replication (folk, 1973), the majority of the viral genomes are covalently joined with cellular dna's containing repetitious sequences. upon a shift to 31 degrees c, free viral genomes appear and are replicated. coupled with the ... | 1976 | 185409 |
| polyadenylate sequences of human rhinovirus and poliovirus rna and cordycepin sensitivity of virus replication. | the polyadenylate [poly(a)] content of the genome rna of human rhinovirus type 14 (hrv-14) is nearly twice as large as that of the genome rna of poliovirus type 2. the poly(a) content of viral rna was determined to be the rnase-resistant fraction of 32p-labeled viral rna extracted from purified virions. polyacrylamide gel electrophoresis indicated that the poly(a) sequences of hrv-14 are more heterogenous and on an average larger than those of poliovirus rna. on the basis of susceptibility to mi ... | 1976 | 185411 |
| [comparative characteristics of the lipoproteins of the human vascular wall and blood plasma]. | electrophoresis in the polyacrylamide gel and ultracentrifugation with a multistage density gradient demonstrated virtually the whole of the blood plasma lipoproteids spectrum to be contained in the human aortal wall. an immunological identity of the whole beta- and alpha-lipoproteids and also of their individual apoproteins in the aortal wall and blood plasma was ascertained. moreover, the ratio of etherified cholesterol/total cholesterol in the vascular and plasmatic lipoproteids proved to be ... | 1976 | 185447 |
| the effect of changes in potassium concentration on the maximal steroidogenic response of purified zona glomerulosa cells to angiotensin ii. | | 1976 | 185465 |
| inhibition of actions of glucagon in adipocytes by gastric inhibitory polypeptide. | possible interactions between gastric inhibitory polypeptide (gip) and glucagon were investigated in rat adipocytes. gip was nonlipolytic and inhibited lipolysis stimulated by glucagon but not that stimulated by secretin or vasoactive intestinal polypeptide (vip). gip competed with 125i-glucagon for binding to adipocyte receptors, and at physiologic concentrations inhibited the stimulation of amp produced by glucagon. thus gip acts as an inhibitor of actions of glucagon on adipocytes and may be ... | 1976 | 185484 |
| sv40 dna sequences as an example of the structure of genes functioning in animal cell nuclei. | recent studies of the structure of messenger rna have demonstrated the existence of untranslated sequences of the 3' and 5' end of the messages. in addition analysis of transcription in vitro has indicated that the nucleotide sequence u6 purine may be part of a transcription termination signal in prokaryotes. recently it has been possible to determine the sequence of extensive portions of the dna of sv40 virus. this article reviews the analogies between certain of these sequences and sequences a ... | 1976 | 185508 |
| [screening of newborns for inborn errors of galactose metabolism. methods and results]. | three inborn errors of galactose metabolism as known today. only two of them cause illness: the deficiencies of galactokinase and of galactose-l-phosphate uridyltransferase. both can be detected through mass screening of newborns and are amenable to a simple and successful dietary treatment. mass screening of newborns for disorders of galactose metabolism can be performed efficiently and inexpensively if it is incorporated in the general newborn screening program. | 1976 | 185513 |
| atp-ppi exchange activity of progesterone receptor. | progesterone receptor preparations from avian oviduct catalyze a pyrophosphate (ppi)-exchange reaction between atp and 32p-labeled ppi. the reaction requires atp exclusively and is mn++-dependent. this enzyme activity is detectable in receptor preparations that have been purified extensively by chromatography on atp-sepharose and deae-sephadex columns. polyacrylamide gel electrophoresis of purified preparations reveals a comigration of [3h]progesterone-receptor complex and the enzyme activity. t ... | 1976 | 185612 |
| inhibition of the glucose induced insulin release by somatostatin in the isolated perfused rat pancreas. action of cyclic amp, glucagon and glibenclamide. | insulin release in the perfused isolated rat pancreas was measured after stimulation with 16.5 mm glucose with and without somatostatin (cycle form, 100 ng/ml) in the medium. a complete blockage of the typical biphasic pattern of insulin release ocurred with somatostatin in the medium. such blockage was abolished when camp (2.5 mm) and a 0.5 ml solution of glucagon (1 mg/ml) were continuously perfused for 20-minute periods and for 30-second periods correspondently. it did not take place when gli ... | 1976 | 185668 |
| systemic stress in the production of cardiac thrombosis in hypercholesterolaemic rats. | an experimental study to elucidate the effects of feeding a high fat diet, hypercholesterolaemia and superadded acute and chronic stress on the production of cardiac thrombosis was carried out in rats. the high fat diet contained 25 percent butter rat providing 45 percent calories from fat which produced significant increase of serum cholesterol and other lipids. acute stress was induced by two injections of adrenaline on a single day which caused a marked transient alteration of haemodynamics. ... | 1976 | 185673 |
| histochemical changes effected by aldosterone on compensatory renal growth in the rat. | the activity of succinic dehydrogenase, adenosine triphosphatase, alkaline phosphatase and cytochrome oxidase was studied histochemically in the remaining kidney of male albino rats which had been subjected to unilateral nephrectomy and were subsequently treated with aldosterone. a marked increase in the activity of succinic dehydrogenase, adenosine triphosphatase and alkaline phosphatase was noted on the initial postoperative days. the increase noted in succinic dehydrogenase activity is consid ... | 1976 | 185676 |
| [bursting pressure of colon in the rats and proteinase inhibition (author's transl)]. | in the early postoperative period (third postoperative day) the colonic enterotomies show 45 per cent higher bursting pressure following administration of aprotinin than the control group (p less than 0,02). on the fifth postoperative day no difference was noted between both groups. the interpretation of the results is difficult because specific parameters such as collagen content and collagenase activity were not determinated. the relationship between colonic anastomotic breakdown and collagena ... | 1976 | 185677 |
| [micro-epidemiology and micro-plaque titration of infectious bovine rhinotracheitis virus on primary calf kidney cells by means of immunofluorescence]. | immunofluorescence was used to study the virus of infectious bovine rhinotracheitis and the course of infection in cell cultures of calf kidney. an indirect relationship was found to exist between the magnitude of inoculation and the onset of specific fluorescence. fluorescent plaques are formed as a result of inoculate dilution. the plaques will grow along with incubation time. release of virus into the culturing medium will first lead to the formation of secondary plaques, then followed by gen ... | 1976 | 185980 |
| extracellular enzymes of the genus bacteroides. | the extracellular production of hyaluronidase and chondroitin sulfatase was demonstrated in all of the subspecies of bacteroides fragilis tested with the exception of b. fragilis subsp. vulgatus. elastase was found only in one strain of b. coagulans tested. this appears to be the first report of these enzyme activities in this genus. additional enzymes found to be produced by certain members othis genus were fibrinolysin, penicillinase, lysozyme, lecithinase, deoxyribonuclease, phosphatase, prot ... | 1976 | 186484 |
| treatment with gentamicin monitored by serum antibiotic assay. | gentamicin is very widely accepted as the most effective antibiotic for treatment of a number of serious gram-negative infections, but one disadvantage associated with its use is the risk of toxicity particularly affecting the eighth nerve. an account is given of eight years' experience with this antibiotic in which obvious ototoxicity was avoided in hospitalized patients in whom gentamicin treatment was monitored by assay of the residual amounts of antibiotic present in the serum. the relations ... | 1976 | 186696 |
| the distribution and clearances of hormones and metabolites in the circulation of the foetal sheep. | plasma hormone and metabolite concentrations have been measured in the plasma of blood collected simultaneously from the femoral artery, umbilical vein and carotid artery of the exteriorized foetal sheep. the concentration of vasopressin and catecholamines was consistently lower and of glucose, lactate and corticosteroids consistently higher in the umbilical vein compared with the femoral artery. acth concentrations showed no consistent pattern and fluctuated widely at each site, but during syna ... | 1976 | 186838 |
| functional and ultrastructural studies of the effects of human interferon on cell membranes of in vitro cultured cells. | the effect of human leukocyte interferon on cultured u-amnion cells was examined, and several biological parameters were registered. multiplication of vesicular stomatitis virus and the virus-produced cytopathogenic effect was prevented. the growth rate of uninfected cells was reduced, as well as the spontaneous release of 3h-uridine. these effects were observed following treatment with 10 units of interferon per ml. no morphological alterations could be detected by scanning electron microscopy ... | 1976 | 187008 |
| [cutaneous herpesvirus infections and malignant blood disorders. epidemiology]. | one hundred and ten skin infections with herpes virus were seen in a uniform group of 1,002 lymphoma-leukaemias dominated by non-hodgkin's lymphoma (385) and hodgkin's disease (327). they appeared with a significantly increased frequency in the course of hodgkin's disease, and between the ages of 60 and 69 for the other groups. in the hodgkin's cases they appeared characteristically during complete remission and in the others during the active phase of their disease. only exceptionally was there ... | 1976 | 187272 |
| cyclic adenosine 3',5'-monophosphate levels and activities of adenylate cyclase and cyclic adenosine 3',5'-monophosphate phosphodiesterase in pseudomonas and bacteroides. | a modified gilman assay was used to determine the concentrations of cyclic adenosine 3',5'-monophosphate (camp) in rapidly filtered cells and in the culture filtrates of pseudomonas aeruginosa, escherichia coli k-12, and bacteroides fragilis. in p. aeruginosa cultures, levels of camp in the filtrate increased with the culture absorbance (3.5 to 19.8 x 10(-9) m) but did not vary significantly with the carbon source used to support growth. intracellular concentrations (0.8 to 3.2 x 10(-5) m) were ... | 1977 | 187575 |
| the protective action of disodium cromoglycate on alveolar macrophages in phagocytosis of fibrogenic silica. | | 1976 | 187887 |
| hypophosphatemia: mouse model for human familial hypophosphatemic (vitamin d-resistant) rickets. | a new dominant mutation in the laboratory mouse, hypophosphatemia (gene symbol hyp), has been identified. the hyp gene is located on the x-chromosome and maps at the distal end. mutant mice are characterized by hypophosphatemia, bone changes resembling rickets, diminished bone ash, dwarfism, and high fractional excretion of phosphate anion (low net tubular reabsorption). phosphate supplementation of the diet from wearning prevents the appearance of severe skeletal abnormalities. the hypophosphat ... | 1976 | 188049 |
| [the concept of risk in poliomyelitis (author's transl)]. | in poliomyelitis the risk is of neurological accidents resulting either from the circulation of wild viruses (spontaneous risk) or from the introduction of virus-vaccine (vaccine risk). the spontaneous risk varies both according to the virus type and according to various factors which determine host resistance. well known among these are: the intrinsic characteristics of the population at a given period (genetic factors and previous experiences of this population with poliomyelitis viruses); env ... | 1976 | 188083 |
| [influence of tobacco smoking on the development of experimental pulmonary tuberculosis]. | the present investigation was designed to the study, in rabbits, of morphologic and enzymatic pulmonary alterations produced by tobacco smoke and the course of tuberculosis under these conditions. the histologic examination revealed muciparous hyperplasia and pavementing metaplasia of the bronchic epithelium, accompanied by interstitial cellular reactions, reticulin, fibrilogenesis, atelectasis, emphysema and marked debility of the animals. in the animals treated with tobacco smoke the tuberculo ... | 1976 | 188112 |
| rotavirus-associated gastroenteritis in two adults probably caused by virus reinfection. | rotavirus infection was diagnosed by virus detection and by serological methods in 2 women with acute gastroenteritis, aged 22 and 29 years, respectively. both patients had been in close contact with children with rotavirus gastroenteritis. rotavirus-specific antibodies were detected in serum specimens obtained prior to the illness in one of the patients, and the serological response in both patients suggested a reinfection with rotavirus as cause of the disease. | 1976 | 188119 |
| localization and activity of tissue bound cyclic nucleotide phosphodiesterase in normal and lack of changes in psoriatic human skin. | this study has been undertaken to elucidate the localization and the activity of cyclic nucleotide phosphodiesterase (pde) in psoriatic epidermis compared to normal. the results showed that the evaluation of cytochemical methods may be difficult because of the various factors which interfere with the reaction and the considerable amount of background staining. additionally, only the tissue bound particulate enzyme fraction may be demonstrated by cytochemical means. nevertheless, the method did r ... | 1976 | 188439 |
| high-affinity cytochalasin b binding to normal and transformed balb/3t3 cells. | to study the molecular basis of changes in sugar uptake rate in cultured mouse fibroblasts with different physiological states, we have measured the high affinity binding of [3h] cytochalsin b, a potent sugar transport inhibitor, to actively growing and contact inhibited balb/3t3 cells as well as to 3t12 and sv3t3 cells. binding was the same whether the cells were detached from dishes with edta or trypsin. the amount of drug bound to intact cells measured with a centrifugation assay was essentia ... | 1976 | 188845 |
| schistosomiasis of the spinal cord. | four cases of spinal cord schistosomiasis were characterized by paraparesis, sensory loss, and sphincter disturbances progressing over hours to days. one patient showed deterioration over months and remission after laminectomy, followed by the typical, rapidly progressive deficits. biopsies on two patients showed granulomatous and necrotizing myelitis. spontaneous improvement and unremitting deterioration, despite chemotherapy, make evaluation of treatment difficult. a survey of all previously r ... | 1977 | 189253 |
| cholecalciferol metabolites binding in porcine parathyroid glands. | we studied the cytoplasmic and nuclear binding of 25-hydroxychole-calciferol and 1alpha,25-dihydroxycholecalciferol inside porcine parathyroid glands. both sterols bind to cytoplasmic components, but a specific nuclear uptake was demonstrated only for 1alpha,25-dihydroxycholecalciferol. these findings support the hypothesis that mammalian parathyroid glands are a target organ for some cholecalciferol metabolites. | 1976 | 189464 |
| detection of adenovirus antigens in sendai virus-induced multinucleate cells by fluorescent antibody technic. | | 1976 | 190337 |
| evidence of nycterohemeral periodicity in stress-induced pituitary-adrenal activation. | plasma acth and corticosterone were measured under basal conditions and after ether or tourniquet stress during the nadir (a.m.) and zenith (p.m.) of the nycterohemeral pituitary-adrenal cycle. exogenous acth was also given at these 2 times to assess adrenal sensitivity to acth and the maximal adrenal capacity for corticosterone secretion. ether stress caused a greater rise in plasma acth in the a.m. than in the p.m., even though basal plasma acth and corticosterone concentrations were lower in ... | 1976 | 190553 |
| [respiratory tract virus infections. possibilities of clinical diagnosis]. | | 1977 | 190561 |
| role of anaerobic bacteria in spontaneous peritonitis of cirrhosis: report of two cases and review of the literature. | the role of anaerobic or microaerophillic bacteria in spontaneous peritonitis of cirrhosis has not been clearly defined. among 126 cases recorded in the literature, in only eight (6 per cent), including the two reported here, was bacterascites associated with anaerobic or microaerophilic bacteria. clinical features in these cases were indistinguishable from those associated with aerobic bacteria. however, polymicrobial bacterascites occurred in four of eight cases associated with anaerobes, as c ... | 1977 | 190883 |
| rat adrenocortical dynamics. | 1. the dynamics of the adrenocortical response to adrenocorticotrophic hormone (acth) was studied in anaesthetized, acutely hypophysectomized male rats. 2. acth test-signals were applied either in a jugular vein ('intact infused preparation') or in the aorta through the coeliac artery with the aorta ligated below this artery ('isolated in situ perfused preparation'). the adrenocortical responses were measured directly in samples serially taken from the left adrenal vein. 3. tested acth signals w ... | 1977 | 191596 |
| bis-basic-substituted polycyclic aromatic compounds. a new class of antiviral agents. 8. bis-basic derivatives of carbazole, dibenzofuran, and dibenzothiophene. | a series of bisalkamine esters, bis-basic ethers, and bis-basic ketones of carbazole, n-ethylcarbazole, dibenzofuran, and dibenzothiophene was synthesized and evaluated for antiviral activity. the series also included two bis-basic alkanes of n-ethylcarbazole and one bis-basic carboxamide of dibenzofuran. structure-activity relationships indicated that within the carbazole and n-ethylcarbazole series the bisalkamine esters gave the most active derivatives while the bis-basic ketone derivatives o ... | 1977 | 191610 |
| factors controlling the activities of phosphatidate phosphohydrolase and phosphatidate cytidylyltransferase. the effects of chlorpromazine, demethylimipramine, cinchocaine, norfenfluramine, mepyramine and magnesium ions. | 1. microsomal membranes from rat liver were incubated with atp, coa, mg2+, [14c]palmitate, f- and sn-glycerol 3-phosphate in order to label them with [14c]phosphatidate. these membranes were isolated and used in a second incubation in which [3h]ctp was present, and the simultaneous synthesis of [14c]diacylglycerol and [3h]cdp-diacylglycerol was measured. 2. the addition of phosphatidate phosphohydrolase, which had been partially purified from the particle-free supernatant, supplemented the activ ... | 1977 | 192211 |
| the epidemiology of the feline leukemia virus (felv). | clustering of cases of feline lymphosarcoma (lsa) has been observed by veterinarians for many years. in 1964 it was discovered that feline lsa was caused by an oncornavirus, the feline leukemia virus (felv). in 1970, a simple, indirect immunoflourescent antibody (ifa) test for felv was developed which enabled large numbers of cats, living in their natural (household) environments, to be tested for the virus. in one study, over 2,000 cats were tested and the results showed conclusively that felv ... | 1977 | 192437 |
| effect of dilution on recovery of bacteria from blood. | the multiplication rate of bacteria in undiluted blood containing sodium polyanethol sulfonate was compared with growth rates obtained in dilutions of blood ranging from 1:2 to 1:8. although all organisms tested grew in the undiluted blood, increased growth rates were seen in the 1:2 dilution. further dilution resulted in growth rates equivalent to that obtained with the 1:2 dilution. in view of these results, we question the present recommendations that blood be diluted 1:10 or 1:20. | 1977 | 192761 |
| anaerobic bacteria in biliary disease in elderly patients. | gallbladder bile from 52 elderly subjects who had undergone biliary tract surgery was examined for the presence of bacteria. twelve patients had sterile bile, 18 specimens of bile yielded anaerobes as well as aerobes, and 22 yielded aerobic bacteria only. escherichia coli was the most commonly isolated organism (30 strains). bacteroides fragilis was the most frequently encountered anaerobic bacterium and was found in 15 patients. the klebsiella-enterobacter group was the second most commonly iso ... | 1977 | 192815 |
| [isolation of preparations of type b monoamine oxidase from liver mitochondria]. | | 1977 | 193582 |
| the effect of shearing of xc dna on its rsv transfecting activity. | xc dna fragments having the average molecular weight of 10(7) daltons were obtained by hydrodynamic shearing followed by sucrose gradient centrifugation or budr treatment of xc cells used for dna preparation. such dna molecules, even after long-term storage, were found to be active in transfection. | 1977 | 193741 |
| latent infection of monkeys with b virus and prophylactic studies in a rabbit model of this disease. | | 1977 | 193814 |
| activation of a procarcinogen to a mutagen by cell-free extracts of anaerobic bacteria. | the salmonella mutagenicity assay can be coupled to cell-free preparations derived from anaerobic bacteria (clostridium perfringens and bacteroides fragilis) to activate a procarcinogen to a mutagen. this activity is destroyed by heating and by digestion with pronase and it is sensitive to oxygen. these findings indicate that the salmonella mutagenicity assay can be adapted to the study of the role of anaerobes in the activation of carcinogens. | 1977 | 197405 |
| substrate positions and induced-fit in crystalline adenylate kinase. | | 1977 | 198550 |
| anaerobes in human biliary tracts. | during a 2-year period, 1,892 patients underwent biliary tract surgery at the mayo clinic. both aerobic and anaerobic cultures of bile were performed in 371 of these patients. sixty-nine percent of the cultures were positive, and 41% (117) of these grew anaerobes, although they were present in pure culture only twice. mixed cultures most commonly contained four different organisms (three aerobes and one anaerobe). bacteroides fragilis was the single most commonly isolated anaerobe and ranked fou ... | 1977 | 200634 |
| the in vitro and in vivo activity of taurolin against anaerobic pathogenic organisms. | an antibacterial agent, taurolin, formed by the combination of 2 molecules of taurine with 3 molecules of formaldehyde has been tested against anaerobes in vitro and in vivo. in vitro, taurolin is highly active against bacteroides species and anaerobic streptococci as measured by the minimum inhibitory concentration. in vivo, small dosages of taurolin given intraperitoneally increase the mortality in mice with bacteroides peritonitis. however, by increasing the dosage of taurolin, survival is pr ... | 1977 | 201040 |
| epstein-barr virus and cytomegalovirus antibodies in infectious mononucleosis. | a method has been evolved for the demonstration of epstein-barr virus (ebv) and cytomegalovirus (cmv) infection in 83 cases of infectious mononucleosis. serum samples were tested for ebv igm, anti-vca igg, anti-ebna, cmv igm and cmv igg antibodies. an acute-phase sample (or samples) and a convalescence sample were examined in each case, and in 44 cases an additional samples was examined 5-12 months after the illness. since the different antibodies showed characteristic differences in both titre ... | 1977 | 201153 |
| evidence for endogenous lh suppression in a man with bilateral testicular tumors and congenital adrenal hyperplasia. | | 1977 | 201659 |
| serologic study on the prevalence of murine viruses in five canadian mouse colonies. | a serologic study was made of five canadian mouse colonies to determine the prevalence of antibodies to 11 murine viruses. a total of 139 sera from the five colonies were evaluated by complement fixation or hemagglutination inhibition methods. viral antibodies were present in all five colonies. antibodies to pneumonia virus of mice, minute virus of mice, theiler's encephalomyelitis virus, and reovirus type 3 were found in all five colonies. k-virus antibodies were present in four colonies. polyo ... | 1977 | 201796 |
| [comparative study of the morphofunctional organization of tissues of different regions of the rat brain in culture]. | the morphological organization and spontaneous electrical activity of neurones of the hippocampus, visual cortex and cerebellum of one- two-day old rats were studied during prolonged cultivation (up to 35 days) of explants under indentical conditions in vitro. differentiation of cells, myelinization of axons and formation of synapses between the neurones were observed. a continuous and periodic types of unit activity were recorded which, for the hippocampus did not depend on the time of cultivat ... | 1977 | 202105 |
| [global program for the elimination of smallpox. iii. the problem of monkey pox as a threat to the global program for the elimination of smallpox]. | | 1977 | 203146 |
| tonsillectomy and infectious mononucleosis. | tonsillar tissue may be the primary site of infection with epstein-barr virus (ebv), the etiologic agent of classical infectious mononucleosis (im). therefore, a retrospective study of the association between tonsillectomy and im was performed in a college student population. in the first part of the study, tonsillectomy rates were compared between 164 im-positive students and 164 student controls who attended ucla in 1972-1975. although it was determined that the development of im during colleg ... | 1978 | 203187 |
| pyrimidine nucleoside monophosphate kinase from human leukemic blast cells. | | 1978 | 203385 |
| detection of alcohols and volatile fatty acids by head-space gas chromatography in identification of anaerobic bacteria. | a head-space gas chromatographic technique for the analysis of volatile bacterial metabolites is described. bacteroides fragilis, clostridium perfringens, and propionibacterium acnes, cultured in a glucose-containing peptone yeast extract medium, were studied. the head-space technique was compared with the injection of the complete liquid culture medium, and solvent extracts thereof, into the gas chromatograph. volatile fatty acids could be detected by all three methods, whereas alcohols produce ... | 1978 | 203602 |
| immunization of chickens against adenovirus infection. | chickens were vaccinated with monovalent and polyvalent adenovirus vaccines using three different serotypes, types 1, 2, and 3. no cross-protection was elicited by the heterologous serotypes but satisfactory protection from lesions and the shedding of challenge virus was induced by the homologous serotypes in the monovalent and polyvalent vaccines. the virus neutralizing (vn) titers were occasionally lower where the polyvalent vaccines were used when compared to those from chickens given the mon ... | 1977 | 203922 |
| anaerobic septicemia after transrectal prostatic biopsy. | transrectal biopsy of the prostate resulted in anaerobic septicemia in two patients, despite parenteral gentamicin sulfate prophylaxis. bacteroides fragilis sepsis developed subacutely in one patient having a postbiopsy pelvic abscess. clostridium perfringens sepsis occurred fulminantly in another patient 24 hours after biopsy of a gland extensively involved with adenocarcinoma. these cases indicate a potential hazard of sepsis due to anaerobic contamination with rectal microflora at the time of ... | 1978 | 204264 |
| ovarian involvement by cytomegalovirus. | | 1978 | 204571 |
| necrotizing pancreatitis due to simian adenovirus type 31 in a rhesus monkey. | simian adenovirus type 31 was isolated from pancreatic tissue of an 8-year-old male rhesus monkey that died as the result of acute necrotizing pancreatitis. histologically, the pancreas showed wide-spread necrosis, extensive infiltration of polymorphonuclear leukocytes, and nuclear inclusions in pancreatic acinar cells. large numbers of adenovirus particles were demonstrable in the acinar cells by electron microscopy. this is the second case of adenovirus-associated pancreatitis we have found in ... | 1978 | 205187 |
| characterization and sequence analysis of a recombination site in the hybrid virus ad2+nd. | | 1978 | 205651 |
| the frequencies of transcription from the e- and l-strands of polyoma dna. | exhaustion type hybridization was used to measure the amount of nuclear virus rna complementary to the early (e) and late (l) polyoma virus dna strands. at 36 h after infection between 2.5 and 7.3% of the newly synthesized virus rna was complementary to the e-strand (-strand) and between 92.7 and 97.5% was complementary to the l-strand (+strand). this proportion was independent of the labelling time, indicating similar accumulation of the e- and l-rna transcripts in the nucleus. the nuclear e- a ... | 1978 | 206660 |
| bone infections involving anaerobic bacteria. | over 700 cases of anaerobic osteomyelitis have been reported in the literature. nonetheless, most reviews of osteomyelitis have paid little attention to the potential role of anaerobes in bone infections. there have, as yet, been no prospective studies of osteomyelitis utlizing optimal anaerobic transport and culture techniques. in a retrospective study of osteomyelitis at wadsworth va hospital from 1973--1975, 39 percent of 58 patients with osteomyelitis had an infection involving anaerobes. an ... | 1978 | 207946 |
| [effect of actinomycin d and daunomycin on the biosynthesis of nucleic acids in cells transformed by oncornavirus and on the biosynthesis of viral nucleic acids (author's transl)]. | | 1978 | 208136 |