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[interactions of low density lipoproteins with glycosaminoglycans and foam cells within the atherosclerotic aorta (author's transl)].very low and low density lipoproteins (vldl and ldl) isolated from aorta's intima in salt solution were predominantly, associated in complex with glycosaminoglycans (gag): hyaluronic acid, chondroitinsulfate a(c), heparin. heparin was detected mainly in complex, isolated from fibrosis plaque. studying of interaction between ldl and foam cells showed that cells in situ as far as under their incubation in medium with llow ldl concentration primarily accumulated lipid of lipoprotein particles. incu ...1979230023
[substrate specificity and kinetic properties of phosphoprotein phosphatase from mesenterial lymph nodes of rats]. 1978230125
comparative studies of two types of "spontaneous" malignant alteration of st/a mouse lung fibroblasts propagated in vitro.two types of apparently spontaneous malignant alterations of fibroblastlike st/a mouse lung cells (st-l cells) grown in vitro are described. one type is characterized by a high tumorigenic potential of the altered cells in nonconditioned syngeneic recipients, a fibroblastlike morphology with cell surface showing very few microvilli by scanning electron-microscopy (sem), and a growth pattern typical of nontransformed cells. these cells were described as r- cells. the other type is characterized b ...1979230149
thymidine kinase enzyme variants in physarum polycephalum. in vitro interconversion of the enzyme variants.isoelectric focusing of plasmodial extracts of physarum polycephalum demonstrated the presence of several multiple enzyme variants of thymidine kinase, which appear sequentially during the nuclear division cycle. variants (a) + (a1) are the only enzyme variants found in the late g2-phase, whereas the variants (c) + (c1) are only present at the time of mitosis and s-phase (1, 2). evidence is presented that multiple forms of thymidine kinase (a) + (a1) with high pi arise by dephosphorylation of a ...1979230187
prevalence of nine different micro-organisms in the female genital tract. a comparison between women from a venereal disease clinic and from a health control department.in a study of the prevalence of nine different micro-organisms in the female genital tract in a swedish population, significantly higher isolation rates occurred among women attending a venereal disease clinic than among those attending a gynaecological health control department. the prevalence of candida albicans, however, was similar in different groups, individual susceptibility being the most important factor. chlamydia trachomatis, trichomonas vaginalis, and mycoplasma hominis occurred conc ...1979230884
[comparison of the effects of analgesia-producing peripheral and central stimulations in cats. effect of nalorphine (author's transl)].the effects of weak intensity percutaneous peripheral stimulations (sppc) on the transmission of nociceptive messages induced by stimulation of the dental pulp have been studied on anaesthetized cats. 1. the jaw opening reflex (rog) and the evoked potentials in the thalamic center median (cm), by stimulation of the dental pulp, disappear after a mean of 30 min after the start of sppc. 2. these effects are analogous to those obtained by stimulation of the periaqueductal gray matter (st.gc) or by ...1979232725
a recently recognized infectious disease. 1978249422
a simplified technique of vasectomy for heat-check boars. 1979257975
oxacillin-induced lysis of staphylococcus aureus.six clinical isolates of staphylococcus aureus were compared for their relative susceptibilities to the killing effects of oxacillin. three of the strains had minimum bactericidal concentrations which were >10 times the minimum bacteriostatic concentration for this antibiotic and were designated tolerant (tol(+)). the other strains had minimum bactericidal concentrations which were comparable to the minimum bacteriostatic concentration (tol(-)). lysis curves of these strains revealed that the to ...1979258576
antibiotic activity in vitro against methicillin-resistant staphylococcus epidermidis and therapy of an experimental infection.staphylococcus epidermidis is a major pathogen in early prosthetic valve endocarditis and cerebrospinal fluid shunt infections. approximately 10 to 15% of hospital isolates are methicillin resistant. ten clinically significant isolates of the latter were collected for antibiotic studies in vitro and in an experimental infection in animals. time-kill studies of five strains showed gentamicin to be the single most effective antibiotic; however, dwarf colony variants emerged as survivors with two o ...1979259421
[establishment of human relationship in nursing: self reappraisal in the interaction with a cancer patient who was not aware of his condition]. 1979259735
[human need for basic exercise - the bedridden patient]. 1978261380
changes in somatomedin-like activity and concentrations of growth hormone and prolactin in the plasma of castrated growing lambs.somatomedin-like activity was measured in the plasma of growing lambs using the porcine costal cartilage disk assay. plasma concentrations were found to be high initially at 2 days of age (mean potency 1.02 plus or minus 0.13 (sem) units/ml, n = 4) declined significantly by 8 days of age (mean potency 0.65 plus or minus 0.04 units/ml, n = 5, p less than 0.01, analysis of variance). thereafter somatomedin-like activity declined slowly to reach its lowest concentration at 146 days of age (mean pot ...1979261980
open-field performance of rats after endotoxin treatment. 1979262092
evidence that triiodothyronine and reverse triiodothyronine are sequentially deiodinated in man.we have demonstrated that in patients given a single iv injection of t3, rt3, or, to a lesser extent, t4, all labeled with 125i in the outer or phenolic ring, chromatography of serum on columns of sephadex g-25 superfine revealed the presence of a labeled material, separate from the administered hormone and from both iodide and iodoprotein. this peak has been termed pre-t3 because it elutes just before the t3 peak. identification of the various compounds in pre-t3 was carried out by cation excha ...1978263472
further studies on bl-3912a: effects on avoidance behavior of rats with low baselines and on reaction thresholds to electric footshock.rats selected for their low performance baselines in an active avoidance shuttle box task were given various doses of r-(-)-2-amino-1-(2,5-dimethoxy-4-methylphenyl) butane (bl-3912a), s-amphetamine or piracetam. bl-3912a at 1 mg/kg ip had no significant behavioral effects, while 5 and 10 mg/kg significantly increased the number of avoidance responses without affecting responses during the intertrial interval (iti). statistically reliable effects on behavior were not observed following 20 mg/kg o ...1977263545
evaluation of new membranes for hemodialysis: preliminary studies with a polycarbonate membrane.a synthetic polycarbonate (pc) membrane supplied by c.r. bard inc. was assessed as to its clinical usefulness and suitability to regular use with artificial kidney. the permeability of the pc membrane to 11 solutes of increasing molecular volumes (na+, ca++, k+, cl-, hpo4-, urea, creatinine, uric acid, glucose, bsp, cyanocobalamine) was measured in vitro by rotating dialysis cells, as compared to that of cuprophan pt 150. the evaluation of the pc membrane in vivo was carried out during a regular ...1979263960
the fetal autonomic nervous system, the fetal heart rate and the beat-to-beat irregularity.in the exteriorized fetal lamb an experimental study on the influence of the fetal autonomic nervous system upon the heart rate pattern is described by means of blockage of the cholinergic, alpha-adrenergic and beta-adrenergic system. the alpha-adrenergic system proved to have no effect upon heart rate. the beta-adrenergic system had a positive chronotropic effect and the parasympathetic system had a negative chronotropic effect. beat-to-beat irregularity was independent of a functional alpha-ad ...1977264064
dopamine effects on circulation and myocardial oxygen supply.we measured the action of dopamine given intravenously at dosage ranging from 2.5 to 320 micrograms/kg per min in closed chest anaesthetized dogs. dopamine produced a dose-dependent increase in heart rate, cardiac index, mean arterial pressure, total peripheral resistance, pulmonary artery pressure, left ventricular end diastolic pressure, coronary flow and myocardial oxygen consumption. at dopamine dosage of 80-320 micrograms/kg per min, the coronary vascular resistance, the stroke volume index ...1977264201
microcell-mediated transfer of murine chromosomes into mouse, chinese hamster, and human somatic cells.in this report, we describe the production and characterization of proliferating hybrid cell populations generated by fusion of murine microcells with intact mouse, chinese hamster, and human recipient cells. the microcell hybrids so produced contained one to five intact murine chromosomes derived from the microcell donor. these transferred chromosomes were maintained as functioning genetic elements in the hybrid cells. our results firmly establish subnuclear particle-mediated chromosome transfe ...1977264685
[the root-canal filling material "endomethasone". part ii: experimental and histological studies including concluding evaluation]. 1977264725
excision repair of benzo[a]pyrene-deoxyguanosine adducts in baby hamster kidney 21/c13 cells and in secondary mouse embryo fibroblasts c57bl/6j.the formation and excision of benzo[a]pyrene-deoxyguanosine adducts in metabolizing baby hamster kidney cells (21/c13) and secondary mouse embryo fibroblasts (c57bl/6j) was investigated. both diastereomeric adducts, n2-(10-(7beta,8alpha,9alpha-trihydroxy-7, 8, 9, 10 tetrahydrobenzo[a]-7,8,9,10-tetrahydrobenzo[a]pyrene)yl)deoxyguanosine, were detected in both cell lines and both cell lines were capable of excising these lesions, albeit with low efficiency.1977265591
in vitro study of cellular influence on 45ca uptake in developing rat enamel.the in vitro method for culture of molar teeth from eight-day-old rats, as reported in this study, appeared to sustain reasonably normal activity in the cells of the enamel organ and pulp through culture periods of four to eight hours. inhibition of metabolic activity in the explants by addition of 5 mm iodoacetate or 2,4-dinitrophenol to the culture medium, or by heating at 70 c for 10 minutes, did not appear to affect the intensity or pattern of 45ca uptake in the more advanced, rapidly minera ...1977265961
characterization of the integration protein of bacteriophage lambda as a site-specific dna-binding protein.the int protein specified by bacteriophage lambda is required for the recombination event that integrates the viral dna into the host genome at its specific attachment site. using a dna-binding assay, we have partially purified the int protein and studied some of the features of its binding specificity and regulation. the dna-binding activity is attributed to int protein because the activity is eliminated by a nonsense mutation or a deletion in the int gene, and is rendered thermolabile by tempe ...1977266191
different rate-limiting steps in excision repair of ultraviolet- and n-acetoxy-2-acetylaminofluorene-damaged dna in normal human fibroblasts.in normal human cells the amount of excision of ultraviolet damage to dna saturates at high doses. in these cells some chemicals mimic ultraviolet damage as far as their biological and repair characteristics are concerned. one of these chemicals is n-acetoxy-2-acetylaminofluorene. we determined whether the limited repair capacity for ultraviolet damage was affected by treatment with n-acetoxy-2-acetylaminofluorene. to measure repair we determined unscheduled dna synthesis and the number of sites ...1977266195
neurotensin: specific binding to synaptic membranes from rat brain.the binding of neurotensin to synaptic membranes from rat brain was studied at 24 degrees with the use of [3h]neurotensin. the binding was found to be highly specific, saturable, and reversible. values for kd of 2 nm and 0.9 nm were derived from equilibrium and kinetic experiments, respectively. virtually no degradation of neurotensin was observed in the incubation medium after exposure to synaptic membranes under the conditions of the binding studies. competitive inhibition of [3h]neurotensin b ...1977266706
phosphohexosyl components of a lysosomal enzyme are recognized by pinocytosis receptors on human fibroblasts.human beta-glucuronidase (beta-d-glucuronide glucuronosohydrolase, ec 3.2.1.31), like many other glycoprotein lysosomal hydrolases, is specifically taken up from the culture medium by human fibroblasts. prior work has indicated that the enzyme exhibits charge heterogeneity and that "high-uptake" forms, i.e., those rapidly internalized by human fibroblasts, are more acidic than slowly internalized forms. here we present two lines of evidence that the acidic group required for the high-uptake prop ...1977266721
coordinated growth of successively initiated tooth germs in the mouse. 1977266860
[efficiency of amalgam preparation by mechanical mixing using predosed capsules].with regard to a modified capsule program permitting to predose dental materials in stomatological practice, the clinical use of an amalgamator designed for predosed capsules is evaluated from economic and ergonomic aspects. during two and a half years of intensive utilization, this amalgamator has proved very successful. therefore, it may be recommended as an essential element of the capsule system.1977267372
properties of the alpha-glucosidase from various human tissues in relation to glycogenosis type ii (pompe's disease).the physico-chemical and electrophoretical properties of alpha-glucosidases from various human tissues and urine have been studied. there were some differences among peak i enzymes (neutral alpha-glucosidases) obtained from liver, heart, muscle, kidney and urine. these differences are based on different effects of tris(hydroxymethyl)aminomethane and various thermostabilities of the peak i enzymes. electrophoretically, the peak i enzyme activity at ph 6.5 from control tissues displayed a two-band ...1977267530
[bioavailability of fluorides from various salts and from various dietary components].in six male subjects fluoride bio-availability after oral application of naf and mgf2 was examined and the influence of food on fluoride absorption was studied by recording fluoride level in plasma and parotid saliva for 24 hours. in addition, fluoride profiles in the plasma of four beagle dogs after the intake of fluoride as naf, mgf2 and caf2 with and without addition of milk and dairy products were established. there were great differences in extent and speed of absorption among the different ...1977267572
[prevalence, location and extent of alveolar bony fenestration and dehiscence in dry human skulls (author's transl)]. 1977267685
iodoamino acids in abnormal and grossly normal thyroids: comparison between 125i and 127i distribution.patients with thyroid cyst (tc), non-toxic nodular goiter (nng), follicular adenoma (fa) and papillary carcinoma (pc) were given a tracer dose of 125i 40h prior to surgery. tissue specimens were hydrolysed with pronase and their labeled iodocompound distribution determined by paper or thin layer chromatography; they were then derivatized and their stable iodoamino acids (iaa) determined by gas liquid chromatography (glc). specific activity (sa) of mit, dit and t4 was within the same range in tc, ...1977267712
why can't you achieve adequate regional anesthesia in the presence of infection?observed in this study were morphologic changes in inflamed nerves, along with biochemical changes, which appear to act concurrently to deactivate or prevent activation of the local anesthetic solution. morphologic changes were observed along the nerve fiber distant from the inflammatory site. these neuro-degenerative changes were seen at the axon and myelin sheaths level. the biochemical data support the presence in the inflamed nerve of amino acids which may be the product of lysosomal rupture ...1977267886
ligands and oxidants in ferrihemochrome formation and oxidative hemolysis.we have investigated the effect of size of a single neutral ring substituent on the induction of hemolytic anemia and the formation of a ferrihemochrome by substituted phenylhydrazines. the severity of induced anemia decreased with increase in size of a halogen atom or an alkyl group ortho to the hydrazino group, little anemia resulting from 2-iodophenylhydrazine and no anemia from 2-tert-butylphenylhydrazine. the size of a halogen atom or an alkyl group at the meta or para position had relative ...1977267949
the philadelphia chromosome (ph1) in adults presenting with acute leukaemia: a comparison of ph1+ and ph1-patients.to evaluate the frequency and clinical significance of the philadelphia chromosome (ph1) in adult acute leukaemia, bone marrow chromosomes were studied in 15 adults with acute lymphocytic leukaemia (all) and 55 with acute nonlymphocytic leukaemia (anll). morphology, clinical findings, therapeutic response and survival were compared in patients with and without the ph1. the ph1 was found in six newly diagnosed adults presenting with all. adults with ph1+ all differed from those with ph1-all in be ...1977268208
effects of antitubulins on the redistribution of crosslinked receptors on the surface of fibroblasts and epithelial cells.substrate-attached normal mouse fibroblasts, transformed mouse fibroblasts (l strain), and epithelial cells (mptr strain) were incubated with two ligands crosslinking different groups of the surface receptors: concanavalin a and cationic ferritin. surface-attached ligands were revealed by an indirect immunofluorescence method. incubation of control cells with these ligands induced the patching of corresponding surface receptors and the clearing of these receptors from the surface zones located o ...1977268637
ion activities in reversible ischemia of the cat brain. 1977268751
enucleation and reconstruction of interferon-producing cells.enucleation of l cells leads to loss of the capacity to produce interferon, showing that the cell nucleus is essential for interferon formation. however, when the cells were enucleated while interferon formation. however, when the cells were enucleated while interferon formation was proceeding, the cytoplasts were capable of continuing to synthesize interferon by a process shown to be protein synthesis, showing that the interferon messenger rna leaves the nucleus after synthesis. reconstructed c ...1977269399
microsurgical vasovasostomy: a reliable vasectomy reversal.optimal results of repair of a severed tube can be expected when a meticulous atraumatic microsurgical technique is based on a detailed knowledge of the anatomy and function of the tube. a reliable vas repair after vasectomy involves the avoidance of leakage or scarring in a tube of which the ends differ in internal diameter from 1 mm to 0-2 mm and in which the inner mucosal layer is difficult of access because of its solid muscular core surround. in addition, the healing will only occur without ...1977269700
postdoctoral research training in cariology: an opportunity initiated by the national caries program at the university of minnesota school of dentistry.in response to the need for development of measures to effectively control human dental caries, a postdocroral research training program in cariology has been established at the university of minnesota school of dentistry. this article discusses the concept of cariology, the background leading to the development of the program, and the goals and structure of the program. the recruiting and training of individuals to perform and direct multidisciplinary basic and applied research in cariology are ...1977269856
high terminal deoxynucleotidyl transferase activity in the spleen of a patient with chronic myelogenous leukemia in blastic crisis. 1977270270
genetic engineering of nitrogen fixation (nif). 1977270345
the clinical trial in multidisciplinary research.dental research has developed along two separate paths. epidemiological studies can only indicate a cause-disease relationship while laboratory experiments can only be applied to the human situation as a working hypothesis. the final solution can only be arrived at by setting up controlled studies in man, though there are limitations on the possibility of control of some variables in human studies. experimental studies on the formation of pellicles and plaque are described as examples of the use ...1977270465
immunity to attenuated influenza virus wrl 105 infection induced by heterologous, inactivated influenza a virus vaccines.groups of student volunteers were immunized with one of five different inactivated influenza virus vaccines. the concentration of virus in the various vaccines differed by both the international unitage test and by the concentration of haemagglutinin, as measured by the single radial diffusion test; the results of the two methods of standardization showed no correlation. the serum hi response to immunization was variable; volunteers given a/england/72 showed a 16.6-fold increase in homologous se ...1977270523
studies on the metabolism of mimosine. 1977270551
submandibular gland enlargement. 1977270565
musculoskeletal tumors in dogs. 1978272367
bacterial meningitis in children.cases of bacterial, non-tuberculous meningitis among auckland children aged one month to 13 years were reviewed for the five year period september 1971 to september 1976. the aetiological agent was established in 203 of 227 cases. haemophilus influenzae was the most frequent cause and together with streptococcus pneumoniae accounted for all sequelae s. pneumoniae was responsible for most fatal cases. it is an unusually common cause of meningitis in auckland, particularly in children under a year ...1977272567
[effect of surgical interventions in the region of the hard palate on the development of the maxilla]. 1978272753
immunization of rats with polypeptide chains from torpedo acetylcholine receptor causes an autoimmune response to receptors in rat muscle.four polypeptide chains were purified from acetylcholine receptor of torpedo californica electric organ. their apparent molecular weights were 64,000, 57,000, 49,500, and 38,000. rats immunized with any of the four chains produced antibodies that crossreacted with rat muscle receptors in vivo. specificities of anti-chain sera were evaluated in vitro by reaction with native receptor solubilized from electric organs and muscles of several species and by inhibition of this reaction with the purifie ...1978273239
hormonal control of progesterone receptors.uterine progesterone receptors are under dual hormonal control. estrogen increases the concentration of receptor through a mechanism that depends on synthesis of both rna and protein. progesterone decreases the concentration of its own receptor, probably by enhancing its inactivation rate. this regulation explains receptor variations during the estrous cycle. in both guinea pig and rat uteri, cytosol receptor concentration is maximal at the preovulatory period and decreases after ovulation. nucl ...1977281173
dupuytren's disease presenting as palmar pits.a 37 year old man with a non-hodgkin's lymphoma and a history of heavy alcohol use presented with bilateral palmar pits. similar lesions were noted in several family members, some of whom had limited use of the hands. although the patient was referred for evaluation of the palmar pits of the basal cell nevus syndrome, close evaluation revealed the diagnosis of dupuytren's disease.1978284078
nongonococcal urethritis. 1979286194
genital tract-to-eye infection: tissue culture of chlamydia trachomatis.a method of cell culture using hela 229 cells is described for the isolation of chlamydia trachomatis. from 40 patients with non-specific urethritis (nsu) attending a venereal disease clinic, the isolation rate was 60 percent and 78 percent from neonates with sticky eyes from which no other organism could be isolated.1979292852
etiology of nongonococcal urethritis. evidence for chlamydia trachomatis and ureaplasma urealyticum.chlamydia trachomatis, ureaplasma urealyticum (t-mycoplasma), and hemophilus vaginalis have previously been considered possible etiological agents in nongonococcal urethritis (ngu). in this study, current c. trachomatis infection was confirmed by culture and (or) micro-immunofluorescence serology in 26 of 69 men experiencing afirst episode of ngu, and 1 of 39 with no urethritis. serum igm immunofluorescent antibody to chlamydia was demonstrated in 16 of 20 men with chlamydia culture positive ngu ...1977300742
myasthenia gravis, dermatomyositis, and polymyositis: immunopathological diseases. 1977302085
spontaneous cytotoxicity of human lymphoblast cell lines mediated by normal peripheral blood lymphocytes. i. differential susceptibility of t-versus b-cell lines. 1977302756
the completion of spermatogenic cells in the course of spermatogenesis in immature rats. 1977303205
the requirement of viable thymocytes for species-specific attachment to and release from macrophages.the role of the thymocyte in its species-specific binding to macrophages has been explored. although formalin treatment of macrophages resulted in loss of binding to thymocytes, formalin treatment of thymocytes did not have this effect. however, two differences between living and formalin-treated thymocytes were noted. formalin-treated thymocytes bound to macrophages of any species whereas the binding of living thymocytes was species specific. living thymocytes attained maximum binding in approx ...1977303248
influence of specificity of reagents and of behaviour of cell surface ig on the detection and enumeration of immunoglobulin-bearing lymphocytes in humans.some parameters likely to influence detection and classification of human b-lymphocytes using anti-immunoglobulin (ig) sera have been investigated. of 20 separate mono- and polyspecific native or conjugated anti-ig sera analysed by a passive haemagglutination technique, 13 exhibited non-specific reactivity. this technique showed no consistent correlation between the titre of individual sera against fab2 and whole igg respectively. the indirect immunofluorescence (if) method applied to detect sur ...1978307583
variation in shape of the human kidney in ontogenetic development. 1978308913
apparatus for uniaxial testing of soft human tissues in vitro. 1978309051
the use of cycloheximide-treated cells for isolating trachoma agents under field conditions.standard procedures for the isolation of chlamydia trachomatis require pretreatment of the tissue culture cells. we evaluated the use of cycloheximide, an antimetabolite that can be added to the cells with the inoculum. cycloheximide-treated cells provided a sensitive system for isolating trachoma agents. this system was applicable to field studies as requirements for equipment were minimal and the cells were 2 weeks old when inoculated.1978310363
[the influence of cryoprotective solutions on the ultrastructure of rabbit corneal endothelium (author's transl)].a series of rabbit corneae was preserved at -196 degrees c in liquid nitrogen using five different cryoprotective solutions: (1) 5% dextran t 40, (2) 10% polyvinylpyrrolidone, (3) 5% dextran t 40 + 10% polyvinylpyrrolidone + dimethyl sulfoxide, (4) 25% rabbit albumin + 10% polyvinylpyrrolidone, (5) 25% rabbit albumin + dimethyl sulfoxide. after thawing the corneae, the influence of the different cryoprotective solutions on the ultrastructure of corneal endothelium was evaluated and compared with ...1978311165
antagonism of hyperthermogenic agents by l 8027, an inhibitor of prostaglandin and thromboxane synthetase. 1979313058
[non-gonorrheal urethritis in men. clinical aspects, diagnosis and treatment]. 1979316550
sexually acquired reactive arthritis. 1979318102
recovery of chlamydia and genital mycoplasma transported in sucrose phosphate buffer and urease color test medium.urethral swabs from 75 males with urethritis were extracted into tryptose phosphate broth and then equal aliquots were dispensed into vials containing sucrose phosphate buffer (2sp) and urease color test medium (u-9). no antibiotics were present in the media. after transport to the laboratory, the recovery of chlamydia trachomatis and ureaplasma urealyticum was evaluated after inoculation into mccoy's cell cultures and agar medium, respectively. c. trachomatis was recovered from significantly mo ...1977319081
screening of blood donors for the detection of antitetanus antibodies suitable for the production of human antitetanus immunoglobulin.during a 12-month period, 40 146 blood donors were tested for the presence of high titre antitetanus antibodies. an attempt has been made to define the most suitable donors, those with antitetanus antibody titres of 4 iu/ml or above, by virtue of titre, sex, location, and length of time since most recent immunisation. particular note is made of those donors from university sessions. towards the end of the series, donors who had an initially acceptable level of antitetanus antibody were being t ...1977321480
treatment of tric infection of the eye with rifampicin or chloramphenicol.an open trial was carried out on 63 patients in london to assess the efficacy of 1% rifampicin eye ointment in comparison with 1% chloramphenicol eye ointment in the treatment of sexually transmitted tric infection of the eye. patients included were selected on the basis of positive cultures for chlamydia trachomatis. three weeks' treatment with rifampicin eye ointment used 3 times daily was not sufficient to cure the disease, but a 6 or 7 week course gave 90% clinical and microbiological cure r ...1977322700
modification of the microimmunofluorescence test to provide a routine serodiagnostic test for chlamydial infection.a modification of the microimmunofluorescence test to provide a practicable routine serodiagnostic test for detecting and characterising chlamydial infection is described which uses four antigen pools, one of which corresponds with each of the four main clinical and epidemiological types of chlamydial infection. the three subgroup a chlamydia (chlamydia trachomatis) pools are: pool 1, hyperendemic trachoma tric agent serotypes a, b, and c; pool 2, paratrachoma tric agent serotypes d, e, f, g, h, ...1977326816
chlamydiales: properties, cycle of development and effect on eukaryotic host cells. 1977334482
b memory cells in the thymus: part of the pool of potentially circulating memory cells.immunization of mice with sheep red blood cells (srbc) or escherichia coli lipopolysaccharide (lps) induces the appearance of b memory cells in the thymus. in this paper the origin of these b memory cells was investigated. therefore, mice primed with either srbc or lps 6 months previously and nonprimed mice were joined for parabiosis. four weeks later the parabiotic mice were separated from each other. another 3 weeks later thymus cells from the primed and nonprimed mice were transferred separat ...1977334972
infections due to chlamydia. 1977336858
chlamydial infections (first of three parts). 1978340951
chlamydial infections (third of three parts). 1978342952
parasite-specified phagocytosis of chlamydia psittaci and chlamydia trachomatis by l and hela cells.phagocytosis of the 6bc strain of chlamydia psittaci and the lymphogranuloma venereum 440l strain of chlamydia trachomatis by l cells and hela 229 cells occurred at rates and to extents that were 10 to 100 times greater than those observed for the phagocytosis of escherichia coli and polystyrene latex spheres. both species of chlamydia were efficiently taken up by host cells of a type they had not previously encountered. phagocytosis of chlamydiae was brought about by the interaction of parasite ...1978344217
gentamicin use and pseudomonas and serratia resistance: effect of a surgical prophylaxis regimen.an outbreak of prosthetic valve endocarditis due to methicillin-resistant staphylococcus epidermidis prompted a change in antimicrobial prophylaxis for open heart surgery in a general hospital from a regimen of aqueous penicillin g, methicillin, and kanamycin to a 5-day regimen of cefazolin and gentamicin. as a result, total gentamicin use in the hospital more than doubled. increased resistance of pseudomonas and serratia isolates paralleled the increased total use of gentamicin. for pseudomonas ...1978348094
lack of correspondence between virus-induced human leukocyte interferon and concanavalin a-induced human migration inhibitory factor (mif). 1978348158
regulation and significance of amino acid metabolism in skeletal muscle. 1978350636
rapid detection of experimental e. coli endophthalmitis by the limulus lysate test.experimental e. coli endophthalmitis was produced in rabbits. the limulus lysate test was applied to aqueous and vitreous samples at various intervals after the intravitreal injection of e. coli organisms. results indicated that this test is feasible using vitreous and aqueous samples. the limulus test was positive for e. coli endotoxin within hours after infection, requiring only 1 hr to determine the presence of endotoxin after sampling. this test may have some value in the rapid diagnosis of ...1978350804
temperature-dependent variation in the synthesis of streptococcal group-specific carbohydrate. ii. biosynthetic studies in group a and variant strains.the biosynthesis of the cell wall polysaccharide and peptidoglycan of group a and a-486-var streptococci was studied with n-acetyl-[14c]glucosamine, udp-n-acetyl-[14c]glucosamine, and [14c]glucose. the incorporation of n-acetyl-[14c]-glucosamine into the cell wall four times greater in the a-486-var cells than in the group a cells. however, the percentage of the total label incorporated into the cell wall polysaccharide at 37 degrees c by the a-486-var strain was 12%, compared with 66% for the g ...1978350826
outer membrane-dependent transport systems in escherichia coli: turnover of tonb function.recent reports demonstrated that the energy-dependent step of vitamin b12 uptake into cells of escherichia coli rapidly declines after cessation either of the expression of the tonb gene or of general protein synthesis. it is shown here that inhibition of protein synthesis results in the decline, with similar kinetics, of all tonb-dependent processes, including sensitivity to colicins b and ia, irreversible adsorption of phage phi80, and siderophore-mediated iron uptake. the role of ongoing tonb ...1978350836
intestinal apoproteins during fat absorption.to compare the roles of apolipoprotein (apo) a-i, b, and e (or arginine-rich apoprotein, arp) in the intracellular production of intestinal chylomicrons (and/or vldl), these apoproteins were localized in rat intestinal mucosa by the light microscope method of indirect immunofluorescence. in addition, tissue levels of apoa-i and apob were measured during fat absorption by radioimmunoassay. antisera were produced using apoa-i isolated from rat plasma high density lipoprotein, and apob and arp from ...1978350901
[physicochemical properties of l-asparaginase with antitumor activity (author's transl)]. 1978351095
immune surveillance and tumors of the nervous system.the theory of immune surveillance postulates that one function of the immune system is to eliminate small numbers of malignant cells that arise spontaneously within the organism. although there has been a great deal of both clinical and experimental evidence in favor of thistheory as it applies to general oncology, the question of whether or not such a surveillance system would be effective for tumors arising within the nervous system has never been studied. the young of pregnant rats which had ...1978351150
use of a heavy inoculum in the in vitro evaluation of the anti-staphylococcal activity of 19 cephalosporins.the in vitro activity of 19 cephalosporins against 105 clinical isolates of staphylococcus aureus and s. epidermidis was determined by using a heavy inoculum, i.e., 10(8) to 10(9) organisms per ml, to maximally challenge the antibiotics. the anti-staphylococcal activities of cephaloridine and 87/312 were consistently decreased by the use of a heavy inoculum when compared with the activity obtained with two less-concentrated inocula. the activity of most of the other compounds was also decreased ...1978352261
the relationship between the spot gene, the synthesis of stable rna, ribosomal proteins, and the beta beta' subunits of rna polymerase following a nutritional shiftup of escherichia coli.the level of ppgpp and rates of synthesis of stable rna, ribosomal protein, and the beta and beta' subunits of rna polymerase were measured following a nutritional shiftup in escherichia coli strains, nf 929 (spot+) and nf 930 (spot-). in the spot+ strain, ppgpp levels decreased 50% within 2 min following shiftup, and the rates of synthesis of stable rna, ribosomal proteins, and the beta and beta' subunits of rna polymerase increased with little or no lag. in contrast, in the spot- strain, ppgpp ...1978352488
immediate cytotoxicity of chlamydia trachomatis for mouse peritoneal macrophages.the toxicity of chlamydia trachomatis was studied with mouse peritoneal macrophage culture. inoculation of 30 inclusion-forming units of trachoma b/tw-5/ot organisms and 250 inclusion-forming units of lymphogranuloma venereum l2/434/bu organisms per cell caused immediated toxicity, with the killing of 40 to 90% of the macrophages within 6 h after inoculation. inhibition of phagocytosis by adsorption at 0 degrees c or by naf pretreatment of macrophages prevented the toxicity, indicating that chla ...1978352950
serotyping of chlamydia: antibodies to lymphogranuloma venereum strains compared by microimmunofluorescence and neutralization tests.the microimmunofluorescence test with hyperimmune rooster sera against lymphogranuloma venereum strains gave the same serotype patterns obtained in the standard mouse serum system. results in a plaque reduction test appeared similar when only 3 prototype strains were tested, but differed when a battery of 11 strains were tested.1978352964
coupling of escherichia coli lipopolysaccharide to epoxy-activated sepharose 6b.escherichia coli o111:b4 lipopolysaccharide was coupled to epoxy-activated sepharose 6b. the bound lipopolysaccharide was immunogenic and immunoadsorptive although at less efficiency than free lipopolysaccharide.1978352965
the time factor in carcinogenesis. the 1977 sievert lecture. 1978353001
chlamydial infections in paediatrics. 1978356747
rapid serological test for diagnosis of chlamydial ocular infections.a rapid serodiagnostic test for the diagnosis of paratrachoma (tric ophthalmia neonatorum, inclusion conjunctivitis, tric punctate keratoconjunctivitis, and trachoma of sexually transmitted origin) has been developed. the technique is based on using a modified micro-immunofluorescence test for detecting antichlamydial igg and igm in the blood and igg and iga in tears. the blood samples are collected on cellulose sponges after a finger prick, and tears are collected by introducing small sponges i ...1978356871
chlamydia trachomatis from men with non-gonococcal urethritis. simplified procedure for cultivation and isolation in replicating mccoy cell culture.chlamydia trachromatis was cultivated on replicating mccoy cells without the addition of antimetabolites. a further technical modification was centrifugation of the specimens at room temperature at 4000 g, thus making it possible to use the method in any microbiological laboratory. c. trachomatis was isolated from 36 of 81 patients (44.4%) with non-gonococcal urethritis. this rate compares well with reported isolation rates using antireplicative agents and higher centrifugation temperatures.1978358749
single-antigen immunofluorescence test for chlamydial antibodies.a simple method is presented for producing large numbers of inclusions of chlamydia trachomatis serotype l2 in cell cultures on slides for immunofluorescence antibody staining. preliminary results with a total of 1,241 human sera from different groups were consistent with findings by earlier methods: 82% of chlamydia-positive men with nongonococcal urethritis had antibodies at titers of greater than or equal to 8, with a geometric mean titer (gmt) of 44.9; 68.5% of chlamydia-negative men with no ...1978359585
rapid diagnosis of chlamydial infection of the cervix.a rapid serodiagnostic test for the presumptive diagnosis of chlamydial infection of the cervix has been developed. the method used in based on the modified micro-immunofluorescence test using pooled chlamydial antigens and the detection of different immunoglobulin classes of chlamydial antibody in sera and cervical secretions. the presence of igg chlamydial antibody at a level of 1/64, or igm antibody at a level of 1/8 or greater, or both in sera and igg or iga antibody at a level of 1/8 or mor ...1978367526
a simple method for the multiple tissue culture assay of chlamydia trachomatis. 1978368489
identification of tubulin from the yeast saccharomyces cerevisiae.a tubulin-like protein was identified in the lower eukaryote saccharomyces cerevisiae. the following criteria were used: (i) copolymerization of the 35s-labeled yeast protein with porcine brain tubulin; (ii) immunoprecipitation of the 35s-labeled yeast protein with antiflagellar tubulin antibody; (iii) the presence of the yeast protein as a constituent of isolated yeast nuclei; and (iv) splitting of the yeast protein in a gel electrophoretic system containing sodium dodecyl sulfate that resolved ...1978368805
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