inhibition of lymphocyte blastogenesis by factor(s) in alloxan-diabetic rat plasma.supplementation of rat lymphocyte cultures with plasma from alloxan-diabetic rats produced a dose-dependent suppression of mitogen-induced blastogenic responses. viability measurements indicated that this inhibition was not due to a direct cytotoxic effect of alloxan-diabetic plasma on rat mononuclear leukocytes in vitro. this inhibition was not explained by hyperglycemia alone and was observed when blastogenesis was induced in vitro by phytohemagglutinin (pha), concanavalin a (con a), or alloge ...1976179906
interaction of chlamydia trachomatis organisms and hela 229 cells.the infection of hela 229 cells in monolayer culture with trachoma (b/tw-5/ot) and lymphogranuloma venereum (lgv) (l2/434/bu) organism was studied in terms of two parameters: radioactivity counts of cell-associated tritium labeled organisms at the initial stage of inoculation for measurement of attachment, and inclusion counts of infection cells after incubation for measurement of growth. factors affecting attachment and inclusion formation and correlation of the two are presented. it was shown ...1976179950
daily variation in concentration of plasma corticosteroid in hypophysectomized rats.a daily variation in the concentration of plasma corticosteroid hormone is present in hypophysectomized rats implanted with pellets of adrenocorticotropic hormone (acth) and thyroxine. although acth is necessary to maintain a functional adrenal, and thyroxine is required to permit the expression of the plasma corticosteroid rhythm, a normal daily variation in plasma corticosteroid concentration does not appear to depend on the periodic release of pituitary hormones.1976179796
reduction of 5'-nucleotidase activity in rat thyroid and adenohypophysis following methylthiouracil treatment.5' -nucleotidase activity was determined in rat thyroid and some other organs employing a specific assay method. during the course of methylthiouracil (mtu) treatment, thyroid 5'-nucleotidase activity decreased significantly. this decrease was specific for this enzyme since the activity of neutral phosphatase did not change and the activity of alkaline phosphatase and mg2+-activated adenosine triphosphatase increased markedly. the 5'-nucleotidase activity of the adenohypophysis also decreased fo ...1976179798
acceptor proteins in rat androgenic tissue chromatin.fractionation of chromatin into urea-soluble chromosomal nonhistone proteins (up), histones (hp), and dna-associated nonhistone proteins (np) revealed that the np fraction from testicular and prostatic chromatin contains organ-specific acceptors for complexes of 5alpha-dihydrotestosterone (17beta-hydroxy-5alpha-androstan-3-one) and its receptor. this acceptor capacity of androgenic tissue chromatin could be transferred to chromatins from non-target tissues with the np fraction of dna-associated ...1976180532
new evidence for the activation of factor xii by epinephrine. 1976180627
a noradren aline sensitive adenylate cyclase in the rat limbic forebrain: preparation, properties and the effects of agonists, adrenolytics and neuroleptic drugs.a method for the preparation of a noradrenaline sensitive adenylate cyclase from homogenates of the rat 'limbic' forebrain is described using krebs--ringer as the homogenising medium. some of its properties resemble those reported previously by other workers, using slices. its response to agonists show that it has the characteristics of a beta1-receptor i.e. the potency of 1-isoprenaline exceeds that of 1-noradrenaline which exceeds that of 1-adrenaline. structure--activity analysis of the respo ...1976179826
sensitivity of rhinoviruses to human leukocyte and fibroblast interferons.rhinoviruses were tested for sensitivity to human interferon from two sources: human leukosytes induced with sendai virus and human diploid fibroblasts induced with polyriboinosinic-polyribocytidylic acid. twenty-five serotypes of rhinovirus were tested against fibroblast interferon in hela cells, and five serotypes were evaluated in fibroblast cultures against fibroblast and leukocyte interferons. sensitivity varied widely in the hela cell assay; rhinovirus type 10 was inhibited by approximatel ...1976180194
properties and partial purification of an oestrogen receptor from neonatal rat brain. 1976182324
[burkitt's lymphoma (author's transl)].burkitt's lymphoma occurs endemically in central africa, but is also found sporadically in areas with temperate climate. a case of burkitt's lymphoma found in our area is described. the literature in regard to the clinical symptoms, etiology and treatment of burkitt's lymphoma is reviewed. at the present time cyclophosphamide is considered to be the drug of choice; it leads to a remission rate of about 90%.1976180474
effect of zinc ions on synthesis of herpes simplex virus type 2-induced polypeptides. 1976181763
standardization fo a digestion-filtration method for isolation of pancreatic islets.standardization of a technic for isolating large numbers of pancreatic islets is described. this procedure employed collagenase digestion of rat pancreatic tissue in a cylindrical wire screen in order to separate isolated islets from undigested pancreas. from this basic protocol the following conditions were established: (1) the duration of the initial digestion period was found to be optimal at six minutes; (2) three subsequent digestions of one minute each effected maximum islet yield; (3) the ...1976182606
cross-linking studies on the state of association of apo a-i and apo a-ii from human hdl. 1976183777
effect of anti-lymphotoxin on cell-mediated cytotoxicity. evidence for two pathways, one involving lymphotoxin and the other requiring intimate contact between the plasma membranes of killer and target cells. 1976186197
[differences in the enzymatic activity of mitochondria from enriched neuronal and glial fractions].studies were performed in the activities of certain enzymes from oxidoreductase group: cytochrome c-oxidoreductase (ec, succinate dehydrogenase succinates: cytochrome c-oxidoreductase (ec, cytochrome oxidase (ec and malate dehydrogenase (ec in mitochondria from neuronal and glial-enriched fractions. the mitochondrial fraction purity was observed by the electron microscope. the enzyme activity of the glial mitochondrial fraction was much higher than that in ...1976181884
quantitative high-performance liquid chromatography of diazepam and n-desmethyldiazepam in blood.high-performance liquid chromatography on porous silica has been employed to determine diazepam and n-desmethyldiazepam in human blood. for forensic purposes, 1.0 ml of blood is sufficient for a quantitative determination of the benzodiazepines in concentrations above 100 ng/ml. in cases where lower levels, 25-100 ng/ml, are of interest, 2.0 ml of blood together with a somewhat more elaborate extraction procedure are necessary.1976186471
the release of endogenous amino acids from the rat visual cortex.the release of endogenous taurine, gaba, glycine, aspartate, glutamate, glutamine and alanine from the rat visual cortex was measured using a cortical cup technique. the electrocorticogram (ecog) was monitored throughout most experiments. 2. spreading depression, evoked by the dropwise placement of 10% kcl solution on to the brain outside the cup was associated with a significant increase in the release of gaba and glutamine but a marked fall in that of glutamate. the evoked release of gaba and ...1976186588
eeg and behavioral effects of morphine in dogs. 1976186809
regulation of sterol synthesis in adrenal gland of the rat by both high and low density human plasma lipoproteins. 1976186062
effects of 5-bromodeoxyuridine on the acth-dependent mitochondrial biogenesis in cortical cells of fetal rat adrenals in tissue culture.cortical cells of fetal rat adrenals in tissue culture were treated with 5-bromodeoxyuridine (brdu) during their proliferative phase and during acth stimulation when nuclear dna synthesis has almost ceased. pretreatment with 0.5 mug/ml/day of brdu inhibited the acth-induced differentiation of cortical cells as well as the secretion of corticosterone and 18-oh-deoxycorticosterone (18-ohdoc). when nuclear dna synthesis was suppressed and mitochondrial dna synthesis was stimulated by acth brdu addi ...1976186465
purification and properties of an endonuclease from nuclei of uninfected and polyoma-infected 3t3 endonuclease activity has been purified approximately 800-fold from nuclei of 3t3 cells infected with polyoma virus. the purfied enzyme catalyzes an endonucleoytic cleavage of single- and double-stranded dna and single-stranded rna. evidence that the activity towards these substrates resides in the same protein molecule is provided by the finding that they co-sediment in sucrose gradients and have identical rates of heat inactivation. studies on the dnase activity shows that the rate of hydro ...1976187596
relative responsiveness (rho): a critical analysis of a new method in receptor differentiation.the rho ratio, which measures the capability of a receptor system to induce a maximal contraction after saturation by a full agonist was analysed from the standpoint of its variability considering the values obtained either for a given receptor system in the same type of preparation or for different types of preparation. this variability was discussed on the grounds of receptor theory, as the basis for a new method in receptor differentiation.1976185120
interrelationships between the central nervous system and patterns of adrenocorticotropic secretion following acute exposure to severe environmental conditions. 1976187557
naturally occurring lymphocyte reactivity to purified mammary tumor virus antigens. 1977191607
low-density beta-lipoproteins (ldl) in swine: investigations of linkage relationships. 1976188358
isolation and localization of rna fractions able to transfer tumor-specific delayed hypersensitivity in vitro.rna fractions were prepared by sucrose density gradient centrifugation from hot-cold phenol, rna-rich extracts of lymphoid tissues from strain 2 guinea pigs hyperimmunized to line 1 or line 10 tumors. each rna fraction was assessed for its ability to convert nonsensitized strain 2 peritoneal exudate cells to a state of specific sensitivity for line 1- or line 10-solubilized tumor antigens. an rna fraction residing between the 4 s and 18 s peaks, designated as fraction "b", transferred line 10 or ...1976187327
glucocorticoid and thyroid hormone-binding to nuclear ribonucleoprotein particles. 1977193730
effects of estrogen, glucocorticoid, glucagon, and adenosine 3':5'-monophosphate on catalytic activity, amount, and rate of de novo synthesis of hepatic histidase.the mechanisms by which estrogen, glucocorticoid, glucagon, and adenosine 3':5'-monophosphate (camp), regulators which participate in the postnatal development of rat liver histidase, elevate the catalytic activity of this enzyme have been explored. a monospecific antibody against homogeneously purified preparations of rat liver histidase has been elaborated in the goat. employing this antibody in immunotitration experiments, it has been demonstrated that the elevations of hepatic histidase acti ...1977193832
regulation of sterol synthesis in 15 tissues of rat. ii. role of rat and human high and low density plasma lipoproteins and of rat chylomicron remnants. 1977193842
divalent cation binding properties of bovine brain ca2+-dependent regulator protein. 1977193856
modulator binding protein. bovine brain protein exhibiting the ca2+-dependent association with the protein modulator of cyclic nucleotide phosphodiesterase. 1977193860
the storage lipids in tangier disease. a physical chemical study.the physical states and phase behavior of the lipids of the spleen, liver, and splenic artery from a 38-yr-old man with tangier disease were studied. many intracellular lipid droplets in the smectic liquid crystalline state were identified by polarizing microscopy in macrophages in both the spleen and liver, but not in the splenic artery. the droplets within individual cells melted sharply over a narrow temperature range, indicating a uniform lipid composition of the droplets of each cell. howev ...1977193870
isolation of chlamydia trachomatis from infant lung tissue. 1977193002
the developmental cycle of chlamydia trachomatis in mccoy cells treated with cytochalasin b.the growth of a genital trachoma-inclusion conjunctivitis agent strain of chlamydia trachomatis in mccoy cells treated with cytochalasin b was studied by quantitative infectivity estimations and by light and electron microscopy. provided that infection of the monolayer was initiated by centrifuging the infectious particles on to the cells before incubation, this chlamydial strain grew as fast and to as high a titre [approximately 10(7) inclusion-forming units (i.f.u.) per culture] as those chlam ...1977195005
regulation of norepinephrine release from cardiac sympathetic fibers in the dog by presynaptic alpha- and beta-receptors.the effect of phenoxybenzamine (pba), desmethylimipramine (dmi), clonidine (clnd), sotalol (stl), and isoproterenol (ispr) on the release of endogenous norepinephrine (ne) from the heart on right cardioaccelerator nerve stimulation was studied in anesthetized dogs. under control conditions, the catecholamine levels in coronary sinus blood increased linearly with increasing frequencies of stimulation up to 10 hz and did not increase further at 30 hz. the release of ne was markedly enhanced after ...1977193655
chlamydia and cmv in pneumonitis. 1977196193
further observations on the effect of repeated electroconvulsive shock on the behavioural responses of rats produced by increases in the functional activity of brain 5-hydroxytryptamine and dopamine. 1977196304
the effect of penicillin on genital strains of chlamydia trachomatis in tissue culture. 1977191429
[effect of the nuclease of serratia marcescens on multiplication of candida tropicalis]. 1977196164
effects of cobalt-60 gamma-irradiation in association with prostaglandin e1 treatment on the cyclic amp levels of some radiosensitive tissues.the amounts of cyclic amp in brain, liver and intestinal mucosa have been measured in rats, at constant intervals, up to 18 days after whole-body exposure to either a unique moderate dose (500 rd) or a unique lethal dose (750 rd) of cobalt-60 gamma-radiation in association with a preliminary intraperitoneally treatment with prostaglandin e1 (5 microgram/100 g body weight/day) during five days. the amounts of tissular cyclic amp in these two experimental groups were compared with those obtained f ...1977201052
effect of estrogens on the acitivities of cholesteryl ester synthetase and cholesteryl ester hydrolases in pigeon aorta.this study is the first to report the effect of conjugated equine estrogens on the acitivity of cholesteryl ester synthetase and cholesteryl ester hydrolases in the aorta. in spontaneously atherosclerosis-susceptible white carneau pigeons, estrogens significantly decreased (p less 0.01) the activity of cholesteryl ester synthetase and increased (p less than 0.01) the cholesteryl ester hydrolase activity in the microsomal fraction of the aorta. there was no effect on the cholesteryl ester hydrola ...1977201055
[importance of the results of a virological examination of sewage. a quantitative evaluation of the finding of enteroviruses in sewage].in order to evaluate quantitatively the effectiveness of virological examinations of sewage, a labeled virus in a dose of 7.5x10(9) pfu was introduced into the canalization system of a community. such an amount of virus could be found in the canalization system if 5--10% of the residents would excrete it simultaneously. the labeled virus was selectively detected in the community sewage. this procedure has demonstrated that the routine methods for sewage collection, specimen treatment, and virus ...1977201104
ultrastructural study of thyroid medullary carcinoma.this study deals with 11 cases of thyroid medullary carcinoma of which 7 were familial cases including 3 cases of sipple's syndrome and 4 cases of sporadic cases. there were also 2 cases of cushing's syndrome. in addition to the previously described english literatures about human medullary carcinoma of the thyroid, the rod-shaped body with cristae and an abundant glycogen particles in the cytoplasm, nuclear inclusion bodies of cytoplasmic invagination and microvilli at the surface membrane of g ...1977201148
epstein-barr virus and cytomegalovirus antibodies in infectious mononucleosis.a method has been evolved for the demonstration of epstein-barr virus (ebv) and cytomegalovirus (cmv) infection in 83 cases of infectious mononucleosis. serum samples were tested for ebv igm, anti-vca igg, anti-ebna, cmv igm and cmv igg antibodies. an acute-phase sample (or samples) and a convalescence sample were examined in each case, and in 44 cases an additional samples was examined 5-12 months after the illness. since the different antibodies showed characteristic differences in both titre ...1977201153
effects of theophylline and n6,o2-dibutyryl adenosine 3':5'-monophosphate on sympathetic ganglionic transmission in rats.the effects of theophylline and n6,o2-dibutyryl adenosine 3':5'-monophosphate (dbcamp) on the amplitude of the postganglionic action potential during and after a 10 hz repetitive volley, and 50 to 1000 msec after a conditioning stimulus were investigated. the effects of both drugs on some electrophysiological properties of single cells of the isolated superior cervical ganglia of rats were also studied. at low concentrations of theophylline a reversible potentiation of the compound action potent ...1978204771
a linkage study of rabbit serum high density lipoprotein (hdl) allotypes.close linkage between the two allotypic systems of rabbit serum high density lipoprotein (hdl), hl 1 and r 67, has been confidently excluded, the recombination fraction being at least 0-20. no suggestion of linkage between either hdl allotype and the immunoglobulin allotypes a1a2a3/b4b9, or the red blood cell system (rbc) adf was obtained. close linkage was confidently excluded for the hl 1-adf, hl 1-a1a2a3, r 67-adf, r 67-a1a2a3, r 67-b4b9, a1a2a3-b4b9, and b4b9-adf relationships.1977194985
the effect of birth and related hormonal events on the neonatal development of uridine diphosphate glucuronosyltransferase activity towards bilirubin and other substrates in rat liver [proceedings]. 1978205461
enhanced virus replication in mammalian cells exposed to commercial emulsifiers.mammalian cell cultures were used to show that a variety of commercial emulsifiers are capable of enhancing the sensitivity of these cells to infection with several viruses. some emulsifiers were not active as enhancers, and those viruses that responded to the enhancing emulsifiers were single-stranded ribonucldic acid viruses. the double-stranded viruses that were tested were nonresponders.1978205172
control of hormone receptor levels and growth of 7,12-dimethylbenz(a)anthracene-induced mammary tumors by estrogens, progesterone and prolactin. 1977196828
the hepatic microsomal mixed-function oxidase system in man: cofactor effects and the influence of cholestasis.microsomal preparations from 22 surgical specimens of normal, cholestatic or severely diseased human liver were analyzed with respect to the amounts of cytochromes p-450 and b5 and nadph-cytochrome c reductase present. normal human liver contained slightly less nadph-cytochrome c reductase and cytochrome p-450 (mean 102.6 +/- 14.6 nmol/mg of protein per min. and 0.60 +/- 0.10 nmol/mg of protein, respectively) than is found in the adult rat, but wide variations were observed. cytochrome b5 was pr ...1977198527
demonstration of saturation kinetics in the intestinal absorption of vitamin c in man and the guinea pig.intestinal absorption of ascorbic acid is believed to be mediated through a sodium-dependent active transport process in man and in the guinea pig, both species having a nutritional requirement for the vitamin. vitamin c transport was studied in man and in the guinea pig by in vivo intestinal perfusion of concentrations of vitamin c ranging from physiologic to clearly pharmacologic levels. triple lumen intestinal perfusion of seven human volunteers with vitamin c concentrations ranging from 0.85 ...1978209065
possible role of the proximal convoluted tubules of human kidney in chronic glomerulonephritis. a quantitative electron-microscopic study.the amount of forming pinocytic (coated) microvesicles on the apical plasma membrane of kidney proximal tubule cells was assessed in kidney biopsies of 10 patients suffering from chronic glomerulonephritis. a significant correlation was found between the amount of these vesicles and diurnal proteinuria levels (r = 0.889; p less than 0.01). the possible mechanisms of protein reabsorption via pinocytosis in both normal and pathological conditions are considered.1978208014
the h1 class of histone and diversity in chromosomal structure. 1978209584
inhibition of t4 polynucleotide kinase by the atp analog, beta, gamma-imidoadenylyl 5'-triphosphate.the inhibition of t4 polynucleotide kinase by beta,gamma-imidoadenylyl 5'-triphosphate has been investigated. it was found that the atp analog was a competitive inhibitor with regard to atp and a noncompetitive inhibitor with regard to dna possessing a 5'-hydroxyl group. at ph 8.0, the ki values were 3 and 11 mm, respectively. beta,gamma-imidoadenylyl 5'-triphosphate was not a substrate in the forward reaction, but would replace adp and atp in the reverse reaction. the reverse reaction was also ...1978210816
the serine protease from rat liver and hepatoma 8999. location and role in mitochondrial protein degradation.1. hepatoma 8999 showed extremely high activity of serine protease, but similar activities of other lysosomal proteases to those of normal rat liver. 2. serine protease from rat liver formed a single immunoprecipitation band against antiserum to purified protease from hepatoma 8999. 3. the serine proteases in rat liver and hepatoma 8999 were restricted to the inner membranes of the mitochondrial fraction. 4. polyacrylamide gel electrophoresis with sodium dodecylsulfate showed that hepatoma 8999 ...1978209014
isotope-tapping experiments with rabbit liver fructose bisphosphatase.isotope-trapping experiments with mental-free rabbit liver fructose 1,6-bisphosphatase have shown that enzyme-bound d-fructose 1,6-bisphosphate completely dissociates prior to enzyme turnover initiated by mn2+ as the catalytic metal. the exchange rate of the binary enzyme-d-fructose 1,6-bisphosphate complex with the substrate pool is, therefore, more rapid than its conversion to products, suggesting that structural mn2+ is necessary for productive substarate binding. rapid-quench isotope-trappin ...1978207315
sendai virus infection in a small mouse breeding colony. 1978213634
purification of bacterial cytochrome c by isoelectric focusing. 1978213681
absence of cytotoxic effect of selected pathogens on hla b27 positive fibroblasts. 1978213785
broiler-breeder vaccination against infectious bursal disease and persistence of maternal antibody in progeny. 1978210922
diagnosis and etiology of nongonococcal urethritis.the observation of more than four polymorphonuclear cells (pmn) per high-power field (hpf) in gram-stained smears of urethral secretions was found to differentiate patients with urethritis from patients without urethritis. a urethral discharge was present in 78% of patients with nongonococcal urethritis (ngu). dysuria without demonstrable urethral discharge and with fewer than four pmn/hpf did not appear to fit into the ngu spectrum. ngu is now defined to include men who have negative urethral c ...1978213495
oxytocin release following osmotic activation of oxytocin neurones in the paraventricular and supraoptic nuclei.1. recordings were made from a total of 35 antidromically identified neurones in the paraventricular (pv) and supraoptic (so) nuclei of urethane-anaesthetized lactating rats. during recording plasma osmotic pressure was raised by 12 m-osmole/kg by injection of hypertonic solutions of nacl, licl, or mannitol.2. nine pv neurones (mean firing rate 4.2 +/- 1.0 (s.e.) spikes/sec) were classified as oxytocin cells because they gave a burst of activity before reflex milk-ejections. none of these showed ...1978209173
diglyceride kinase from escherichia coli. purification in organic solvent and some properties of the enzyme.the diglyceride kinase activity of membranes from escherichia coli was extracted into acidic butan-1-ol. the enzyme was purified in organic solvent by precipitation at -20 degrees c, chromatography on deae-cellulose and repeated chromatography on sephadex lh-60. the final 1460-fold purified enzyme preparation gave a single protein band upon isoelectric focusing in the presence of triton x-100 (pi, 4.0) and upon polyacrylamide-gel electrophoresis in the presence of sodium dodecylsulphate. the lat ...1979218816
interaction between haemagglutinating virus of japan and human en (a--) erythrocytes lacking major sialoglycoprotein. 1979218837
s49 lymphoma wild type and variant clones contain normal calcium dependent regulator.calcium dependent regulator is present in wild-type s49 lymphoma cells, in the variant that is deficient in adenylate cyclase activity (ac-), and in the uncoupled variant (unc). the electrophoretic mobility and the ability to stimulate cyclic nucleotide phosphodiesterase of the calcium dependent regulator from each of these three clones are indistinguishable from those of the modulator protein isolated from bovine brain. calcium dependent regulator does not appear to be involved in the defect re ...1978214461
the interaction between mitochondrial nadh-ubiquinone oxidoreductase and ubiquinol-cytochrome c oxidoreductase. evidence for stoicheiometric association.1. the nadh-ubiquinone oxidoreductase complex (complex i) and the ubiquinol-cytochrome c oxidoreductase complex (complex iii) combine in a 1:1 molar ratio to give nadh-cytochrome c oxidoreductase (complex i-complex iii). 2. experiments on the inhibition of the nadh-cytochrome c oxidoreductase activity of mixtures of complexes i and iii by rotenone and antimycin indicate that electron transfer between a unit of complex i-complex iii and extra molecules of complexes i or iii does not contribute to ...1978215122
calcium-dependent 3':5'-cyclic nucleotide phosphodiesterase. inhibition of basal activity at physiological levels of potassium ions.a calcium-dependent cyclic nucleotide phosphodiesterase from rat cerebrum was, in the absence of activator protein, inhibited by various monovalent cations. the inhibition was rapid, readily reversible, and concentration-dependent, with 100 mm cesium, rubidium, or potassium ion inhibiting essentially all basal enzyme activity, while 100 mm sodium or lithium ions produced only moderate inhibition. the potency of the cations in inhibiting the enzyme was cs greater than or equal to rb greater than ...1978214428
secretory granules of the duodenal enterochromaffin cells of the cat. 1979222959
growth and effect of chlamydiae in human and bovine oviduct organ cultures. 1979223717
isoproterenol-induced desensitization of adenylate cyclase responsiveness in a cell-free system. 1979220254
participation of monocyte-macrophages and lymphocytes in the production of a factor that stimulates collagenase and prostaglandin release by rheumatoid synovial cells.cultured mononuclear cells from human peripheral blood produce a soluble factor (mcf) that stimulates collagenase and prostaglandin e2 (pge2) release by cultured rheumatoid synovial cells up to several hundred fold. these target rheumatoid synovial cells lack conventional macrophage markers. to determine which mononuclear cells are the source of mcf, purified populations of monocyte-macrophages, thymus-derived (t) lymphocytes, and bone marrow-derived (b) lymphocytes were prepared. the monocyte-m ...1979227933
abnormal properties of an immediate early polypeptide in cells infected with the herpes simplex virus type 1 mutant tsk.previous studies (r. j. watson and j. b. clements, virology 91:364--379, 1978; c. m. preston, j. virol. 29:275--284, 1979) have shown that the herpes simplex virus type 1 (hsv-1) mutant tsk has a temperature-sensitive lesion in an immediate early polypeptide whose function is to induce synthesis of new viral transcripts, including mrna, for pyrimidine deoxyribonucleoside kinase. the studies presented here examine the properties of immediate early polypeptides in wild-type hsv-1 and tsk-infected ...1979228063
photoaffinity inactivation of the enkephalin arylazide enkephalin derivative, [d-ala2,met5]enkephalin-tyr-n-(2-nitro-4-azidophenyl) ethylenediamine (etn), has been synthesized. in the dark, it inhibited the binding of [3h]enkephalinamide to enkephalin receptor-rich ng-108 cell membranes with an i50 = 2.2 x 10(-8) m or ki = 7 x 10(-9) m, assuming competitive inhibition. photolysis of membranes in the presence of etn caused irreversible inactivation of the enkephalin receptor, but inactivation was prevented by the addition of enkephalin, ...1979227880
role of adrenergic nerves in blood-induced cerebral vasospasm.cerebral arteries have an abundant supply of adrenergic nerve fibers which are believed to release vasoactive substances responsible for the induction of cerebral vasospasm. to assess the importance of adrenergic nerves in this phenomenon, high doses (600 microgram/ml) of 6-hydroxydopamine (6-ohda) were used to produce in vitro chemical sympathectomy in bovine middle cerebral artery. 6-ohda reduced catecholamine fluorescence to undectable limits. h3-norepinephrine re-uptake was reduced to 1.5% o ...1979228448
electromyographic study on experimental hyperkalaemia. ii. effects on neuromuscular transmission. 1978228534
enhancement of post-ultraviolet killing in escherichia coli k-12 through the action of gyrase inhibitors: evidence for associated gyrase-recbc deoxyribonuclease function.this work in conjunction with the results presented in an earlier report (m. a. purdy and k. l. yielding, antimicrob. agents chemother. 10:182--184, 1976) showed the following. (i) nalidixic acid and novobiocin could inhibit post-ultraviolet and post-x-ray survival, implicating gyrase function in deoxyribonucleic acid repair. (ii) the inhibition of post-ultraviolet survival requires the action of functional recbc deoxyribonuclease. (iii) structural changes in the gyrase could (a) cause recbc mut ...1979228590
aflatoxin inhibition of viral interferon induction.the inhibitory effect of four basic aflatoxins on interferon induction by influenza virus in llc-mk2 cell monolayers follows a structure-activity series with decreasing potency in the order aflatoxin b1 greater than g1 greater than b2 approximately g2. of the four aflatoxins, b1 was the most deleterious to both cell growth and the viability of cells in confluent cultures. the fact that higher levels of influenza virus growth were attained in aflatoxin-treated cells than in normal cell monolayers ...1979228592
studies on avian infectious bronchitis virus (ibv). iii. interferon induction by and sensitivity to interferon of ibv.the induction of interferon by avian infectious bronchitis virus (ibv) and the sensitivity of ibv to interferon were studied. the results of experiments with ten ibv strains are summarized as follows. 1. all the ibv strains tested induced interferon in chick embryo (ce) cells, chicken kidney (ck) cells and embryonated eggs. the iowa-609 strain induced about 1000 units of interferon in ce cells while the beaudette-42 strain induced about 200 units of interferon in embryonated eggs; the interferon ...1979228636
isolation of cardiac myofibrils and myosin light chains with in vivo levels of light chain phosphorylation.conditions are described for the preparation of functional myofibrils and myosin light chains from freeze-clamped beating hearts with the state of light chain phosphorylation chemically 'frozen' during the extraction procedure. myofibrils were shown to be functionally intact by measurement of ca2+ binding and atpase activity. highly purified cardiac myosin light chains could be routinely isolated from myofibrillar preparations using ethanol fractionation together with ion-exchange chromatography ...1979228754
interactions between periodate-oxidized adenosine triphosphate and the mitochondrial adenosine triphosphatase [proceedings]. 1979229031
cell-density control of amino acid transport in simian virus 40-transformed 3t3 cells [proceedings]. 1979229032
acceleration of mammary cancer development by grafting of fetal mammary mesenchymes in c3h mice.transplantation of fetal mammary gland mesenchyme into mammary glands of 2-month-old syngeneic virgin mice resulted in focal re-enactment of events that normally occur probably during fetal and early postnatal development of the mammary gland. portions of the recipient's mammary duct system in contact with the fetal mammary mesenchyme underwent branching and proliferation in a pattern resembling that of rudimentary mammary gland development. this process occurred in c3h mice regardless of whethe ...1979229052
synthesis of dna in the liver and testes of cadmium-tested partially hepatectomized rats.cadmium affects the induction of thymidine and thymidylate kinases in regenerating rat liver. edta administered simultaneously with cadmium reverses its inhibitory action on enzyme synthesis, and prevents the depression of thymidine incorporation into dna observed in cadmium-treated animals. zinc does not abolish the inhibitory action of cadmium on the synthesis of dna in regenerating liver, and the incorporation of thymidine into dna in the testes was inhibited more by intraperitoneal injection ...1979229099
effects of fatty acids on growth of bordetella pertussis in defined medium.the effects of saturated and unsaturated fatty acids on the growth of bordetella pertussis strain 114 in defined medium were tested. individual fatty acids were found to be either inhibitory or stimulatory to growth, depending on concentration. myristic (c14), pentadecanoic (c15), and palmitic (c16) acids were the most inhibitory saturated fatty acids tested. b. pertussis 114 was extremely sensitive to the unsaturated fatty acids oleic (c18; cis-9), elaidic (c18; trans-9) and petroselinic (c18; ...1979227923
primate medicine and husbandry. 1979229615
[diagnostic and prognostic value of scintigraphic methods in cardiology (static methods)].the diagnostic possibilities of myocardial scintigraphy in cardiology are discussed. as an invasive technique the perfusion imaging is described. the valuation of the doubleisotope-scintigraphy for better diagnostic examination during coronary arteriography is accentuated. the 201-thallium-scintigraphy as a non invasive method is debated. the diagnostic value of the 201-thallium stress scintigraphy is shown. the similarity of ergometric and pharmacologic provocation with dipyridamol is represent ...1979229649
serum levels of somatomedin a and growth during long-term treatment of patients with pituitary dwarfism with human growth hormone.eighteen patients with pituitary dwarfism were treated for 1 7/12 to 6 years with human growth hormone (hgh) at a dose of 0.19--0.62 unit (u) per kg of body weight per week. the mean increment in height was 2.0 +/- 0.4 and 8.8 +/- 0.5 cm/year before and the first year after treatment of hgh, respectively. a significant positive correlation was observed between serum levels of somatomedin a and growth rate, espcially in children with bone age below 10 and a duration of treatment of less than one ...1979229685
effect of hypophysectomy upon renal kallikrein-kinin system in is confirmed that urinary kallikrein is significantly and persistently decreased in totally hypophysectomized rats. in addition, kallikrein activity in the kidneys in these animals is significantly reduced (p less than .001) as compared to partially hypophysectomized and sham-operated rats. the administration of 125 mu of acth twice a day for 2 months, did not modify urinary kallikrein excretion. negative effects were also obtained in subsequent periods, by injection of 10 microgram corticost ...1979229709
gaba-receptors in the substantia nigra controlling cyclic-gmp in the cerebellar cortex. 1979229716
peripheral vascular disease following radiation therapy.radiation therapy is effective for the treatment of many malignant diseases. this fact is well documented. the existence of complications in any treatment form does not obviate its usefulness. an awareness of possible complications, however, makes our treatment program more realistic and causes us to be more aware of the patient's complaints following therapy. one of our patients complained of claudication for seven years before an arteriogram was done. how many swollen, painful arms after maste ...1979229750
genital chlamydia trachomatis infections in patients with cervical atypia.of 177 gynecologic outpatients with cervical dysplasia studied, 29 (16%) were found to excrete chlamydia trachomatis from their urogenital tract. sera collected from 93 of these patients were significantly more often positive for and showed higher levels of antichlamydial micro-complement fixation (cf) and immunofluorescence (if) antibodies than sera obtained from the controls. however, when the sera were tested for anti-herpesvirus type ii (hsv-ii) and cytomegalovirus (cmv) antibodies, no diffe ...1979224353
calcium-independent and dependent steps in action of clostridium perfringens enterotoxin on hela and vero cells. 1979229852
denaturation of thermophilic ferricytochrome c-552 by acid, guanidine hydrochloride, and heat.the denaturation of thermus thermophilus cytochrome c-552 by acid, guanidine hydrochloride, and heat was studied by measuring the changes in absorption and circular dichroism. cytochrome c-552 was remarkably resistant to acid; the pk of the transition from the low- to the high-spin form was roughly 0.3. the effect of guanidine hydrochloride on the heme iron-methionine bond of thermus and horse cytochromes c was also investigated; a comparison of the free-energy changes for the displacement of th ...1979229899
[reaction between specific antisera and synaptic membrane preparations]. 1979229918
effects of the histamine h2-receptor blocking drugs burimamide and cimetidine on noradrenergic transmission in the isolated aorta of the rabbit and atria of the guinea-pig.1 in rabbit aortic strips, concentration-response curves to noradrenaline (na) were shifted to the right in a parallel and concentration-dependent manner by the alpha-adrenoceptor blocking drug, phentolamine and also by the histamine h(2)-receptor blocking drugs, burimamide and cimetidine. responses to 5-hydroxytryptamine were not affected by these drugs.2 burimamide had the properties of a competitive antagonist of noradrenaline, possessing about one-hundredth the potency of phentolamine. cimet ...1979229935
studies on peptides. lxxxii. synthesis of [4-gln]-neurotensin by the methanesulfonic acid deprotecting procedure. 1979229978
immunization against respiratory diseases in cats. 1979230010
[interactions of low density lipoproteins with glycosaminoglycans and foam cells within the atherosclerotic aorta (author's transl)].very low and low density lipoproteins (vldl and ldl) isolated from aorta's intima in salt solution were predominantly, associated in complex with glycosaminoglycans (gag): hyaluronic acid, chondroitinsulfate a(c), heparin. heparin was detected mainly in complex, isolated from fibrosis plaque. studying of interaction between ldl and foam cells showed that cells in situ as far as under their incubation in medium with llow ldl concentration primarily accumulated lipid of lipoprotein particles. incu ...1979230023
[substrate specificity and kinetic properties of phosphoprotein phosphatase from mesenterial lymph nodes of rats]. 1978230125
comparative studies of two types of "spontaneous" malignant alteration of st/a mouse lung fibroblasts propagated in vitro.two types of apparently spontaneous malignant alterations of fibroblastlike st/a mouse lung cells (st-l cells) grown in vitro are described. one type is characterized by a high tumorigenic potential of the altered cells in nonconditioned syngeneic recipients, a fibroblastlike morphology with cell surface showing very few microvilli by scanning electron-microscopy (sem), and a growth pattern typical of nontransformed cells. these cells were described as r- cells. the other type is characterized b ...1979230149
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