| [chlamydial infection in women with ectopic pregnancy]. | a study was carried out on the presence of antibodies to c. trachomatis in women with ectopic pregnancy, established during laparotomy. pathohistological examination discovered that ectopic pregnancy occurred due to chronic salpingitis in a larger part of the women. the presence of antibodies to c. trachomatis was found in women with more sexual partners more frequently. serum data for infection to c. trachomatis did not correlate with inflammatory gynecological diseases, undergone in the past. ... | 1991 | 1789364 |
| [significance of cervical chlamydia infections in pregnancy for mother and child]. | | 1991 | 1790927 |
| demonstration of chlamydia trachomatis in colposcopic cervical biopsy specimens by an immunoperoxidase method. | a total of 31 cervical biopsy specimens were taken from 29 women attending a genitourinary medicine clinic, nine women (11 biopsy specimens) were known to have chlamydia trachomatis cervicitis and 20 women were known to be free of chlamydial infection. the specimens were routinely processed to paraffin wax and stained by an anti-chlamydia immunoperoxidase technique to localise the organisms. of the 11 positive biopsy specimens three showed positive staining of elementary/reticulate bodies. in on ... | 1991 | 1791204 |
| [epidemiological study on chlamydia trachomatis infection in obstetrics and gynecology field in tokyo]. | in conjunction with the tokyo branch of japan association for maternal welfare, we have been carrying out examinations of chlamydial infection on women, especially pregnant women, living in tokyo. specimens were collected from 212 gyneco-obstetric clinics in tokyo during 4 years from january 1987-december 1990. a total of 13,925 swab specimens from patients who were suspected of sexually transmitted diseases from clinical findings and pregnant women who requested the examination were tested for ... | 1991 | 1791328 |
| [the clinical significance of specific iga antibodies in local secretions in cases with chlamydial urogenital infections--comparison with serum antibodies and analysis of antigen specificity by immunoblotting assay]. | iga antibody titers to c. trachomatis in local secretions were measured by immunoperoxidase assay (savyon kit) in male and female cases with various urogenital infections, and the clinical significance of iga antibody in the local secretion was discussed. in addition, the antigen specificity of the iga for c. trachomatis in the local secretions was analyzed by immunoblotting assay. 1) in female cases with cervicitis and male cases with urethritis, the positive rate of iga antibody in their secre ... | 1991 | 1791344 |
| [studies on uptake of macrolide antibiotics by chlamydia host cell]. | minimum inhibitory concentration (mic) of macrolide antibiotic (ml) against c. trachomatis was found to greatly vary with the cell culture system used for the assay. we then investigated the ability of various cell cultures to uptake mls in the relation to mic determination. penetration of the 14c-labeled mls, erythromycin, jasamycin and rokitamycin into cells was quantitatively studied by measuring the radioactivity incorporated into mccoy, hela229w and hela229f cells. it was found that hela229 ... | 1991 | 1791345 |
| chlamydial infection in riyadh, saudi arabia. | sera of patients attending a genitourinary clinic, gynaecological clinic in al ali general hospital, riyadh, saudi arabia and from males and females without overt genital diseases were tested for the presence of antichlamydial antibodies using an immuno-fluorescence test. antibodies for chlamydia trachomatis were found in 66 (46%) of 145 male patients and 72 (35%) of 200 female patients attending a genitourinary clinic and gynaecological clinic respectively and in two (2%) of 100 men and in none ... | 1991 | 1791413 |
| screening for neisseria gonorrhoeae and chlamydia trachomatis in a donor insemination programme. | cultures of all 4450 specimens of semen from 193 semen donors were negative for n. gonorrhoeae. a microtrak direct fluorescence microscopy test for the detection of ch. trachomatis was used to screen 448 specimens from all 268 recipients of semen on an insemination programme for 3 1/2 years; all pre-insemination endocervical samples and all subsequent endocervical samples were negative. it is considered that the incidence of gonorrhoeae or chlamydia in our recipients or donors is very low. fluor ... | 1991 | 1792339 |
| [significance of vaginal and cervix flora with reference to ascending infections]. | the role of the vaginal and cervical flora and the extent of alterations in the flora in ascending infections of the female genital tract were evaluated in a microbiological study of 86 patients with a clinical diagnosis of pelvic inflammatory disease (pid). the patients were classified as having acute salpingitis (n = 15), an acute inflammatory adnexal tumour (n = 21), subacute salpingitis (n = 38), or chronic salpingitis (n = 12). 100 symptom-free women formed a control group. the spectrum of ... | 1991 | 1792794 |
| should we treat postvenereal reiter's syndrome by antibiotics? | | 1991 | 1795310 |
| function and morphometric evaluation of intrauterine undernutrition on kidney development of the progeny. | renal function and renal morphometry of the progeny of rats submitted to 50% dietary restriction (pair-fed with control group) throughout pregnancy (rt group), during the first half of pregnancy (r1 group) or during the second half of pregnancy (r2 group) were studied 3 months after birth. glomerular filtration rate (gfr) and renal plasma flow (rpf) decreased significantly in all groups when compared to control (c) (gfr, 4.44 +/- 0.12, 4.04 +/- 0.18, and 4.00 +/- 0.16 vs 6.87 +/- 0.17, and rpf, ... | 1991 | 1797294 |
| function and morphometric evaluation of intrauterine undernutrition on kidney development of the progeny. | renal function and renal morphometry of the progeny of rats submitted to 50% dietary restriction (pair-fed with control group) throughout pregnancy (rt group), during the first half of pregnancy (r1 group) or during the second half of pregnancy (r2 group) were studied 3 months after birth. glomerular filtration rate (gfr) and renal plasma flow (rpf) decreased significantly in all groups when compared to control (c) (gfr, 4.44 +/- 0.12, 4.04 +/- 0.18, and 4.00 +/- 0.16 vs 6.87 +/- 0.17, and rpf, ... | 1991 | 1797294 |
| chlamydia trachomatis in pelvic inflammatory disease. | c. trachomatis seems to be an important causative organism of pelvic inflammatory disease (pid) as c. trachomatis antigen and/or antibody was found in 234 (69.85%) of 335 patients clinically diagnosed to have pid. antigen was detected in the lower genital tract of 118 (35.22%) patients whereas igg antibody was present in 188 (56.11%), of whom 89 (47.34%) had a high titre. repeat testing of sera of 37 patients showed a rise in antibody titre in 18 (40%). as the success rate of therapy for pid wit ... | 1991 | 1797644 |
| effects of hypothyroidism upon the granular layer of the dentate gyrus in male and female adult rats: a morphometric study. | the effects of hypothyroidism upon the structure of the central nervous system of adult rats are poorly understood in spite of evidence that the mature brain is vulnerable to this condition. existing developmental studies show that the morphological changes induced by thyroid hormone deficiency are related to alterations in neurogenesis. we studied the granular layer of the dentate gyrus under different experimental conditions of hypothyroidism, because in rodents the neurogenesis of the granule ... | 1991 | 1797872 |
| a new motor test sensitive to aging and dopaminergic function. | neurodegenerative disorders which involve motor impairment is characteristic of old age. although there are a few tests which attempt to assess motor incapacities, many have utilized scales which have either a great deal of subjective evaluations or are subject to learning-performance complexities. this study describes a method able to measure motor impairment of aging rats which is subject to dopaminergic influences and has negligible practice effects. the test is designed so that rats have to ... | 1991 | 1798345 |
| hypomagnesemic hypocalcemia as a cause of persistent upper airway obstruction in acute bacterial tracheitis. | | 1991 | 1800361 |
| hypomagnesemic hypocalcemia as a cause of persistent upper airway obstruction in acute bacterial tracheitis. | | 1991 | 1800361 |
| induced abortion: microbiological screening and medical complications. | preoperative cervical screening of 1,193 women undergoing first-trimester induced abortions yielded chlamydia trachomatis in 11.7%, neisseria gonorrhoeae in 0.8%, mycoplasma hominis in 22.1%, ureaplasma urealyticum in 10.1%, herpes simplex virus in 0.9% and group b streptococci (gbs) in 2.9%. c. trachomatis and n. gonorrhoeae were especially frequent among teenagers. a total of 2.2% (26 women) developed postoperative pelvic inflammatory disease (pid) and 0.9% (13 women) endometritis. pid develop ... | 1991 | 1800368 |
| a primary demyelinating disorder of young cattle. | the clinical signs and neuropathological changes are described in six limousin x calves that, at about one month after birth, showed signs of blindness, nystagmus, rotation of the eyes, opisthotonos, hyperprotraction of the forelegs and, in one case, apparent seizures. pathologically there was necrosis of the optic chiasma and focal areas of myelin sheath vacuolation or demyelination in certain areas of the brain, especially in the cerebellar peduncles. in one animal, kept alive for 7 months, th ... | 1991 | 1800911 |
| vascular factors in the neurotoxic damage caused by 1,3-dinitrobenzene in the rat. | using a 3 x 10 mg/kg dose schedule of 1,3-dinitrobenzene (dnb) over two days in fischer rats, we have found the following changes in vascular function and structure during the early phase of the symmetrical brain stem lesions. 1. marked increase in cerebral blood flow generally but especially in the inferior colliculi, from 6 h after the final dose of dnb. 2. increasing incidence of petechial haemorrhages in inferior colliculi, cerebellar roof, vestibular and superior olivary nuclei from 12 h. 3 ... | 1991 | 1800913 |
| vascular factors in the neurotoxic damage caused by 1,3-dinitrobenzene in the rat. | using a 3 x 10 mg/kg dose schedule of 1,3-dinitrobenzene (dnb) over two days in fischer rats, we have found the following changes in vascular function and structure during the early phase of the symmetrical brain stem lesions. 1. marked increase in cerebral blood flow generally but especially in the inferior colliculi, from 6 h after the final dose of dnb. 2. increasing incidence of petechial haemorrhages in inferior colliculi, cerebellar roof, vestibular and superior olivary nuclei from 12 h. 3 ... | 1991 | 1800913 |
| sleep changes in fasting rats after chronic glycerol feeding. | species which do not enter torpor during fasting and which were efficiently able to spare their body proteins during the first two phases of fasting (which are commonly comprised of 3 successive phases) also increase their daily amount of slow-wave sleep (sws) during the first two phases. since in fasting animals the ability to spare proteins was reported to be improved when they were previously fed with a diet enriched with glycerol, it was supposed that, after such a diet, food-deprived rats w ... | 1991 | 1801006 |
| [age and sex distribution of sexually transmitted diseases in valladolid. a study of 5076 cases]. | the age and sex distribution of 5,076 cases of s.t.d. dealt with between 1982 and 1988 at the dermatological dispensary of the territorial social welfare service in valladolid are studied. the maximum frequency of s.t.d. (24.1% of cases) corresponded to the age group between 21 and 25 years, which was also the period for maximum prevalence of neisseria gonorrhoeae (26.3%), gardnerella vaginalis (30.7%), candida albicans (27.4%) and acuminata condyloma (33.4%). syphilis (19.9%), chlamydia trachom ... | 1991 | 1801185 |
| [effect of human neutrophil elastase, cathepsin--g. superoxide anion and endotoxin on the pgi2 production by cultured bovine pulmonary endothelial cells]. | the effects of human neutrophil elastase (hne), cathepsin-g, h2o2, xanthine oxidase-hypoxanthine derived superoxide anion and endotoxin on the pgi2 production by cultured bovine pulmonary endothelial cells were observed. the results showed that hne, superoxide anion and h2o2 could decrease the pgi2 production by endothelial cells, and cathepsin-g had no effect on the production of pgi2. in our experiment, endotoxin could enhance pgi2 production. it was suggested that hne, superoxide anion, and h ... | 1991 | 1802432 |
| [chlamydia pneumonia (strain twar) infection--a new respiratory tract pathogen]. | | 1991 | 1802444 |
| the immunobiology of mycoplasma arthritidis and its superantigen mam. | | 1991 | 1802617 |
| the immunobiology of mycoplasma arthritidis and its superantigen mam. | | 1991 | 1802617 |
| evaluation of urogenital chlamydia trachomatis infections by cell culture and the polymerase chain reaction using a closed system. | two hundred and fifty-four specimens from males and females consulting a clinic for sexually transmitted diseases were analyzed for genital chlamydia trachomatis infection. each clinical sample was tested by the cell culture technique and the polymerase chain reaction using a closed system. when the two test systems were compared, the overall sensitivity of the polymerase chain reaction was 96% and the specificity 94% when compared to the cell culture technique. by use of a closed system for dna ... | 1991 | 1802697 |
| [risk factors of ectopic pregnancy]. | | 1991 | 1805137 |
| isolation of an infectious oncogenic adenovirus strain from the stimulated lymphocytes of a patient with bladder tumour. | in attempts to grow viruses from tumour cells and circulating lymphocytes, we isolated an oncogenic infectious adenovirus type 18 strain from the phytohaemagglutinin-stimulated peripheral t-lymphocytes of a patient with bladder tumour. the virus genome is assumed to play certain role in the development of the tumour in interaction with other dna viruses and the damaged immune system. | 1991 | 1805504 |
| estimation of a preference matrix for women's choice of male sexual partner according to rate of partner change, using partner notification data. | data from a study of partner notification for venereal chlamydial infection are used to estimate the contact preference matrix, according to sexual contact activity levels of the male partners, of young heterosexual women. a method for correcting data for the bias introduced by studying infected women only is presented. results indicate that an overall proportional mixing model is not an adequate description of the mixing pattern. | 1991 | 1806122 |
| [cytological picture of nasal smears suggestive of chlamydia infection]. | | 1991 | 1808581 |
| women's voices after pregnancy loss: couples' patterns of communication and support. | this paper reports results of a study of prenatal diagnosis patients who lost pregnancies. the women felt they shared much in common with their male partners and generally felt understood and supported by the men. however, many of the women also saw their responses to the loss as different from those of their male partners. a variety of coping patterns were adopted by the women in the face of these differences. implications are considered for preventive efforts to minimize distress; treatment ap ... | 1991 | 1808722 |
| nonrandom chromosome changes in methylnitrosourea (mnu) induced mouse t-cell lymphomas. | since nonrandom chromosome changes in neoplastic cells have proven to be good indicators of the site of gene alterations related to transformation, the authors examined the chromosomes of t-cell lymphomas induced in rf/j strain mice with methylnitrosourea (mnu). all treated mice developed thymic lymphomas within 10 weeks of injection. chromosomes of the thymus cells were examined at intervals before and during lymphoma development, as well as after they were passaged in syngeneic and in nude mic ... | 1991 | 1809237 |
| standardizing laboratory adhesion tests. | laboratory adhesion tests are performed as screening tests prior to the clinical evaluation of dentin bonding restorative systems. these tests include the determination of the bond strength to dentin and the evaluation of microleakage and marginal gap dimensions at the tooth-restoration interface. no standardized adhesion tests are available as the methodologies vary tremendously from one laboratory to another. the variable factors are reviewed in this manuscript and laboratory studies to standa ... | 1991 | 1810333 |
| prevalence of chlamydia trachomatis infections in karachi, pakistan. | an epidemiological study was carried out to determine the prevalence of chlamydia infections in adult females by enzyme immunoassay and microscopic examination of giemsa-stained smears. endocervical swabs were collected from 126 females attending ob/gyn ward at abbasi shaheed hospital, karachi. 13.5% of 126 females tested were positive by enzyme immunoassay and only 5.6% were positive by the giemsa-staining method. the infection rate among pregnant and nonpregnant women with urinogenital problem ... | 1991 | 1812293 |
| [chlamydia diagnosis--important with the right method of specimen taking]. | | 1991 | 1812661 |
| [isolation of chlamydia trachomatis in populations with different risks of infection]. | the level of infection by chlamydia trachomatis in patients attending different units of urogenital diseases was evaluated. one hundred and fifty four patients divided in four groups were studied. fifty percent of c. trachomatis isolation was achieved in the high risk group a, composed by men attending to a sexual transmission disease service. among men attending an urology unit (group b) isolation reached 20.7%. in group c composed by women from a general gynecological unit the rate of isolatio ... | 1991 | 1815276 |
| [effect of the type of swab on the isolation of chlamydia trachomatis]. | the success in the isolation of c. trachomatis is dependent on several factors. one of them is the type of swab used for collection the specimens. as some types of swabs, such as calcium alginate and aluminum, cotton and wood have recently been shown to decrease the isolation rate, different types of swabs were prepared and tested in our laboratory. commercial aluminum, type 5056 aluminum and stainless steel were used with the same type of cotton. commercial swabs of cotton and wood were tested ... | 1991 | 1815280 |
| management of chlamydial cervicitis in general practice. | | 1991 | 1815566 |
| olive milling wastewater as a medium for growth of four pleurotus species. | four species of pleurotus were adapted to grow on olive milling wastewater, and in certain conditions produced high yield of fruit bodies. some biochemical transformations were observed in the olive milling wastewater owing to the growth of pleurotus. in particular, the fungi actively excreted large amounts of laccase in the medium, and at the same time the concentration of phenolics and other toxic compounds significantly decreased, as revealed by hplc analysis and toxicity tests on standard cu ... | 1991 | 1818536 |
| [sexually transmitted diseases and mother-infant transmission (epidemiology and prevention)]. | gonococcal ophthalmia neonatorum may be severe with a high risk of blindness; therefore prophylaxis is mandatory. chlamydial ophthalmia is less severe and prevention remains less effective. both microorganisms may be responsible of preterm labour and perinatal deaths. congenital syphilis is an important public health problem in developing countries. some recent papers underline the decrease of infestation when mothers may be screened during pregnancy (at least two times in initially seronegative ... | 1991 | 1819391 |
| [frequency of the ocular carriage of chlamydia trachomatis by newborns and infants. a multicenter french study]. | a multicenter study has been done in france in order to assess the presence of c. trachomatis in the newborn and the young infant. the results show that 4.3% of the infants less than 2 month old are positive. this put forward the question of the screening of pregnant women. | 1991 | 1819412 |
| [impact of chlamydia trachomatis on pregnant women in gabon]. | during two years 3,312 pregnant women has been screened for c. trachomatis. in spite of an improvement of the way of life and program of information on std the rate of c. trachomatis have increased and reach a percentage of 22%. c. trachomatis is mostly found in women younger than 21 years and give no clinical sign in more than 55%. the consequence of this high degree of infection on the new born is difficult to assess but one cause, stress that 30% of the conjunctivitis are linked to c. trachom ... | 1991 | 1819413 |
| evaluation the enzyme immunosorbent assay ideia test detecting chlamydia trachomatis in cervix. | a total of 87 cervical specimens of unselected female sex workers in massage parlors were tested by an enzyme amplified immunoassay ideia chlamydia test and cell culture for the presence of chlamydia trachomatis. the prevalence of c. trachomatis was 28 (32%) cases and 34 (39%) cases by the cell culture and the ideia chlamydia test respectively. the ideia chlamydia test demonstrated the sensitivity and specificity of 85.7% and 83% respectively, positive and negative predictive values of 70.5% and ... | 1991 | 1820656 |
| [comparison of immunofluorescence, enzyme immunoassay and cell culture for the diagnosis of chlamydia trachomatis in urogenital infections]. | we evaluated the usefulness of the direct immunofluorescence test with monoclonal antibodies and the enzyme immunoassay in comparison with isolation in cell cultures for the diagnosis of chlamydia trachomatis in 55 endocervical specimens from female prostitutes and 21 urethral specimens from men with diagnosis of nongonococcal urethritis. in comparison with culture, the enzyme immunoassay had a sensitivity of 100% and a specificity of 95%. the immunofluorescence test had a sensitivity of 92% and ... | 1991 | 1824159 |
| diagnosis and prevalence of persistent chlamydia infection in infertile women: tissue culture, direct antigen detection, and serology. | specimens for chlamydial culture, direct fluorescent antibody (dfa) test, two enzyme immunoassays (eia) for antigen detection, and serum for chlamydial antibodies were collected from 256 infertile women. specimens were taken from the tubes during tuboplasty and from the cervix and endometrium during laparoscopy or tuboplasty. antibodies to chlamydia trachomatis were found four times more often in patients with signs of prior pelvic inflammatory disease (pid) than in infertile women with normal p ... | 1991 | 1825070 |
| synovial t lymphocyte-specific immune response to chlamydia trachomatis in reiter's disease. | we studied the lymphocyte proliferative response to chlamydia trachomatis in reiter's syndrome (rs) compared with that in other rheumatic diseases. rs patients showed significantly increased c trachomatis-specific synovial fluid (sf) t cell proliferation. proliferating cells were found in both cd4+ and cd8+ t cell subsets. the sf lymphocyte proliferative response to c trachomatis in rs was inhibited by anti-class i and class ii major histocompatibility complex monoclonal antibodies, while the re ... | 1991 | 1827261 |
| protective immunity in pig-tailed macaques after cervical infection with chlamydia trachomatis. | seven pig-tailed macaques were inoculated in the cervix with chlamydia trachomatis. cervical infection resulted in all animals. after spontaneous cessation of cervical shedding of c. trachomatis, repeated inoculation either failed to produce infection, or resulted in infection of shorter duration with lower inclusion counts. thus, cervical infection may lead to protective local immunity in pig-tailed macaques. | 1991 | 1827541 |
| diagnosis and laparoscopic grading of acute salpingitis. | pelvic inflammatory disease continues to be a major cause of morbidity in women of reproductive age. findings of bilateral adnexal tenderness and signs of a lower genital tract infection (mucopus, or leukorrhea, or both) should prompt clinicians to consider the diagnosis of salpingitis in this group of women. additional signs of infection, such as elevated temperature, palpable adnexal complex, leukocytosis, elevated erythrocyte sedimentation rate, or c-reactive protein, and positive tests for e ... | 1991 | 1827704 |
| combined detection of phenotype and y chromosome by immunoenzyme labelling and in situ hybridisation on peripheral lymphocytes. | a novel staining method for simultaneously determining the immunophenotype and sex of peripheral lymphocytes is described. cell surface markers on lymphocytes are identified using specific monoclonal antibodies located by a peroxidase anti-peroxidase staining technique (pap). lymphocytes are subsequently hybridised with a biotinylated y chromosome-specific sequence probe and this is located by avidin-biotin-alkaline phosphatase staining. following the two staining steps the preparation is examin ... | 1991 | 1828475 |
| combined detection of phenotype and y chromosome by immunoenzyme labelling and in situ hybridisation on peripheral lymphocytes. | a novel staining method for simultaneously determining the immunophenotype and sex of peripheral lymphocytes is described. cell surface markers on lymphocytes are identified using specific monoclonal antibodies located by a peroxidase anti-peroxidase staining technique (pap). lymphocytes are subsequently hybridised with a biotinylated y chromosome-specific sequence probe and this is located by avidin-biotin-alkaline phosphatase staining. following the two staining steps the preparation is examin ... | 1991 | 1828475 |
| poly(adp-ribose) polymerase inhibitors suppress uv-induced human immunodeficiency virus type 1 gene expression at the posttranscriptional level. | gene expression of human immunodeficiency virus type 1 (hiv-1) is induced not only by trans activation mediated through a gene product (tat) encoded by the virus but also by treatment of virus-carrying cells with dna-damaging agents such as uv light. employing an artificially constructed dna in which the chloramphenicol acetyltransferase gene was placed under the control of the hiv-1 long terminal repeat, we analyzed the induction process in hela cells and found that inhibitors of poly(adp-ribos ... | 1991 | 1828533 |
| production of colony-stimulating factors during pneumonia caused by chlamydia trachomatis. | the colony-stimulating factors (csfs) are cytokines involved in the production, differentiation, and activation of host phagocytes. during murine infection with chlamydia trachomatis (mopn), plasma csf levels increased in euthymic (nu/+) and athymic (nu/nu) balb/c mice. levels declined later in infection, with the nu/+ mice resolving the infection but the nu/nu mice succumbing by day 16. either live or heat-killed chlamydia organisms could induce csf increases on day 7 postchallenge in nu/+ mice ... | 1991 | 1828791 |
| in vitro folding pathway of phage p22 tailspike protein. | the intracellular chain folding and association pathway of the thermostable, trimeric phage p22 tailspike endorhamnosidase has been the subject of a previous detailed study employing temperature-sensitive folding mutants. recently, reconstitution of native tailspikes from completely unfolded polypeptides has been accomplished, providing a model system to compare protein folding pathways in vivo and in vitro. the in vitro reconstitution pathway of the protein after dilution from guanidine hydroch ... | 1991 | 1828991 |
| in vitro folding pathway of phage p22 tailspike protein. | the intracellular chain folding and association pathway of the thermostable, trimeric phage p22 tailspike endorhamnosidase has been the subject of a previous detailed study employing temperature-sensitive folding mutants. recently, reconstitution of native tailspikes from completely unfolded polypeptides has been accomplished, providing a model system to compare protein folding pathways in vivo and in vitro. the in vitro reconstitution pathway of the protein after dilution from guanidine hydroch ... | 1991 | 1828991 |
| murine cdnas coding for the centrosomal antigen centrosomin a. | screening of an induced ehrlich ascites cell-derived lambda gt11 cdna library with an antibody (gp1), immunoreacting specifically with centrosomal antigen(s) of interphase and mitotic cells of different species, released a partial cdna clone (lambda p10a) encoding the carboxy-terminal section of a centrosome-specific antigen. this specificity of the clone lambda p10a could be verified by lacz-directed antigen expression from escherichia coli y1089 lysogenized with the recombinant phage lambda p1 ... | 1991 | 1829085 |
| [chlamydia infections in gynecology]. | | 1991 | 1829811 |
| single-dose trospectomycin for chlamydial urethritis in men. | trospectomycin is an aminocyclitol analog of spectinomycin with significant in vitro activity against chlamydia trachomatis. a single 1-g intramuscular dose was administered to 10 men with symptomatic, culture-positive chlamydial urethritis. trospectomycin was well tolerated but failed to eradicate chlamydial infection, as determined by cultures obtained approximately 1 week after treatment. | 1991 | 1830196 |
| altering testpack chlamydia sampling swab decreases testing time. | testpack chlamydia protocol time was reduced from 25 min to 15 min by altering the swab used to collect endocervical specimens for direct immunoassay detection of chlamydia trachomatis. the clinical performance of the two testpack chlamydia protocols was statistically equivalent when evaluated with 607 paired endocervical specimens. | 1991 | 1831415 |
| kinetic studies of mg(2+)-, co(2+)- and mn(2+)-activated d-xylose isomerases. | the kinetic parameters for the interconverting substrates d-xylose in equilibrium d-xylulose and d-glucose in equilibrium d-fructose were determined for several d-xylose isomerases, with mg2+, co2+ and mn2+ as metal ion activators. the km, kcat. and kcat./km values are tabulated for the anomeric mixtures (observed parameters) as well as for the respective reactive species, i.e. the alpha-pyranose anomers of d-xylose and d-glucose and the alpha-furanose forms of d-xylulose and d-fructose (real pa ... | 1991 | 1831974 |
| role of l3t4-bearing t-cell populations in experimental murine chlamydial salpingitis. | a role for both the cellular and humoral components of the immune response has been established for chlamydial infection. the significance of helper (l3t4) t cells was evaluated by using a chlamydia trachomatis murine salpingitis model for upper genital tract chlamydial infection. mouse oviducts were inoculated with c. trachomatis by using the mouse pneumonitis agent (mopn) or control medium. mice depleted of l3t4-bearing lymphocytes had significantly higher (p less than 0.05) numbers of organis ... | 1991 | 1832663 |
| expression of fc gamma riii on hela 229 cells: possible effect on in vitro neutralization of chlamydia trachomatis. | the neutralizing activities of a murine immunoglobulin g3 (igg3) monoclonal antibody specific for the major outer membrane protein of chlamydia trachomatis and its monovalent fab fragments were studied by using syrian hamster kidney (hak) cells and human epithelial (hela 229) cells. the intact igg3 antibody was neutralizing for hak cells but was nonneutralizing for hela cells. in contrast, monovalent fab antibody fragments neutralized chlamydial infectivity for both hak and hela cells. immunoflu ... | 1991 | 1832664 |
| [salpingitis]. | the etiology of salpingitis is polymicrobial, however, the particular pathogen is difficult to identify, even in laparoscopically obtained specimens. chlamydia trachomatis, neisseria gonorrhoeae, anaerobes and facultative anaerobic bacteria have to be covered by antimicrobial therapy. this article reviews the current aspects of etiology, diagnosis, therapy, prevention and complications of salpingitis. | 1991 | 1833934 |
| [acute salpingitis. celioscopy before and after treatment: 110 cases]. | the authors report their experience concerning 110 cases of acute salpingitis that were diagnosed and controlled laparoscopically one month after antibiotic treatment had been achieved. during the second look laparoscopy three parameters were appreciated: pelvic adhesions, tubal patency, and residual inflammation. laparoscopic findings at the time of early second look have shown relationship between the occurrence of tubal sequelae and the seriousness of initial pid: the relation was statistical ... | 1991 | 1835469 |
| [prevalence of chlamydia trachomatis infections as a cause of sexually transmissible diseases--studies from a berlin counseling center for venereal diseases]. | in a study of the public health advisory centre for veneral diseases in berlin-schöneberg 207 patients with signs of urethritis and/or cervicitis were examined in antigen- and antibody-tests for infections with chlamydia trachomatis type d-k. on the strength of the high prevalence of 33.3% in the antigen test and 40.1% in the iga antibody test an extension of the std diagnostic by the advisory boards for veneral diseases is demanded by means of an appropriate amendment of the law for the control ... | 1991 | 1837603 |
| [factors affecting the results of culture of chlamydia trachomatis in a mccoy cell culture]. | the authors confirmed that the sensitivity of cultivation of the laboratory strain chlamydia trachomatis, serotype e in a mccoy cell culture is increased by centrifuging of the inoculum and addition of cycloximidine to the cultivation medium. conversely, postcentrifugation sedimentation of the inoculum and addition of glucose to the medium has no effect. to meet the needs of the parasite and the host cells the glucose content in the basic p component of the medium--mem is sufficient. exchange of ... | 1991 | 1838714 |
| phospholipase a2 acyl-hydrolytic activity in rat rpar-induced pleurisy. | endogenous phospholipase a2 (pla2) specific activity (sa) (% hydrolysis/min/mg protein) in the rat pleural cavity, measured as the acyl-hydrolysis of [3h]-arachidonic acid e. coli substrate, was quantitated after induction of a reverse passive arthus reaction (rpar). pla2 sa, derived when the rate of hydrolysis was linear (1-5 min), was 1.9, 1.4, 3.8 and 4.1% h/min/mg at 10 min, 2 h, 4 h and 24 h, respectively, after induction of the rpar. this time course appeared to correlate with the influx o ... | 1991 | 1838900 |
| isoproterenol fails to produce myocardial necrosis in streptozotocin-induced diabetic rats. | biochemical alterations in the hearts of non-diabetic and 5 weeks of streptozotocin-induced diabetic rats following isoproterenol (iso) administration were compared. serum lactate dehydrogenase (ldh) and myocardial adenosine triphosphate (atp), creatine phosphate (cp), lactate and glycogen were used as indices of myocardial injury. hearts from diabetic rats (blood glucose greater than 350 mg/dl), before iso administration, had normal lactate levels but significantly low high-energy phosphate (he ... | 1991 | 1839021 |
| [report of 4 cases of chlamydia infection in the urogenital tract]. | four cases of chlamydia infection of the urogenital tract were received-recently in our out-patient clinic for foreign patients. the 4 patients were two couples. both male patients had histories of coitus with other partners: among these two, one had suffered gonorrhea and the other was suspected of having had gonorrhea. urogenital tract symptoms or sustained abnormal urine analysis results are characteristic of this disease, but in females often no obvious symptoms present. measurements with fl ... | 1991 | 1839529 |
| a microbiological study of genital ulcers in kuala lumpur. | the microbial aetiology of genital ulcers was studied in 249 patients (241 men and 8 women) attending a sexually transmitted disease clinic in kuala lumpur, malaysia. herpes simplex virus type 2 was isolated in 48 (19.2%) patients, haemophilus ducreyi from 22 (8.8%), neisseria gonorrhoeae from seven (2.8%) and chlamydia trachomatis from four (1.6%). syphilis was diagnosed in 18 (7.2%) patients on the basis of dark field microscopy. two (0.8%) patients were found to have both chancroid and syphil ... | 1991 | 1839925 |
| sequence analysis of the major outer membrane protein gene of chlamydia pneumoniae. | compared with the major outer membrane proteins (momps) of the other chlamydial species, the chlamydia pneumoniae momp appears to be less antigenically complex, and as determined by immunoblot analysis, it does not appear to be the immunodominant antigen recognized during infection. nucleotide sequence analysis of the c. pneumoniae momp gene (ompa) revealed that it consisted of a 1,167-base open reading frame with an inferred 39,344-dalton mature protein of 366 amino acids plus a 23-amino-acid l ... | 1991 | 1840574 |
| vectorette pcr: a novel approach to genomic walking. | vectorette pcr (or chemical genetics) is a method that enables the amplification of specific dna fragments in situations where the sequence of only one primer is known. thus, it extends the application of pcr to stretches of dna where the sequence information is only available at one end. in this report, we describe the chemical genetics method and demonstrate its use in one specific application. in addition, we demonstrate how fragments generated by this procedure can be sequenced rapidly and s ... | 1991 | 1842919 |
| chlamydial infection in canada. | | 1991 | 1843663 |
| isolation of chlamydia trachomatis during ivf-et. | the authors isolated chlamydia trachomatis from the granulosa and from the spermatozoa of an infertile couple taking part in the ivf-et program at the department of obstetrics and gynaecology of the university of pisa. | 1991 | 1844324 |
| [etiology of lower respiratory infections in hospitalized infants]. | as a contribution to knowledge about the etiology of lower respiratory tract infections (lri) in infants, 235 patients aged one year or less admitted to a children's hospital at northern metropolitan area of santiago, chile along years 1987 throughout 1989 with radiologically confirmed diagnosis were studied. infants were eligible only if their symptoms lasted for not more than five days and their hospital stay was less than two days. controls consisted on 74 healthy infants. a search for presum ... | 1991 | 1844925 |
| solid phase immunoassay for c trachomatis. | | 1991 | 1845107 |
| diacylglycerol-protein kinase c signalling in skeletal muscle: a possible link to insulin resistance. | | 1991 | 1845146 |
| cytomegalovirus infection in sexually active adolescents. | to determine whether cytomegalovirus (cmv) infection in teenage girls is related to sexual activity, 254 girls 12-18 years old (mean, 15.8) attending a contraceptive counseling clinic were studied. participants were screened for chlamydia trachomatis, neisseria gonorrhoeae, and trichomonas vaginalis, and serum antibody to cmv was determined. demographic and sexual history data were collected by interview. the mean number of lifetime sex partners was 2.2; 173 (68%) were seropositive. race, greate ... | 1991 | 1847399 |
| the bacillus subtilis cytochrome-c oxidase. variations on a conserved protein theme. | the structural genes of cytochrome-c oxidase in bacillus subtilis have been isolated and sequenced. five genes, ctab-f, are closely spaced. ctac, ctad, ctae and ctaf are the genes for subunits ii, i, iii and ivb, respectively, ctab, which may encode an assembly factor, is separated and upstream from the others. in comparison to its mitochondrial counterparts, subunit i has an extended c-terminus with two additional transmembrane segments, whereas subunit iii has lost two such segments from its n ... | 1991 | 1847686 |
| kinetic analysis of transport and opioid receptor binding of [3h](-)-cyclofoxy in rat brain in vivo: implications for human studies. | [3h]cyclofoxy (cf: 17-cyclopropylmethyl-3,14-dihydroxy-4,5-alpha-epoxy-6-beta-fluoromorp hinan) is an opioid antagonist with affinity to both mu and kappa subtypes that was synthesized for quantitative evaluation of opioid receptor binding in vivo. two sets of experiments in rats were analyzed. the first involved determining the metabolite-corrected blood concentration and tissue distribution of cf in brain 1 to 60 min after i.v. bolus injection. the second involved measuring brain washout for 1 ... | 1991 | 1847702 |
| ultracytochemical localization of nad(p)h oxidase activity in the human placenta. | ultracytochemical localization of nad(p)h oxidase activity was demonstrated in the human term placenta by the cerium method. the activity of this enzyme was also compared to those of other oxygen-intermediates-metabolizing enzymes, such as xanthine oxidase, catalase, peroxidase and superoxide dismutase. nad(p)h oxidase activity was exclusively confined to the apical microvillous membrane of the syncytiotrophoblast. other enzymes studied showed no activity. we discuss the possibility that nad(p)h ... | 1991 | 1847711 |
| identification of a novel nuclear protein synthesized in growth-arrested human hepatoblastoma hepg2 cells. | dna synthesis of human hepatoblastoma hepg2 cells is reversibly inhibited by butyrate. when butyrate is removed from the culture medium, cells re-enter the cell cycle, synthesizing dna with a time lag of about 12 h. hepg2 cells, growth-inhibited for 30 h with butyrate, synthesize and accumulate a nuclear protein, called d. protein d synthesis is inhibited in cells which, released from the butyrate block, have resumed dna synthesis as well as in growing cells never exposed to butyrate. protein d ... | 1991 | 1847869 |
| etiology of acute lower respiratory tract infections in gambian children: i. acute lower respiratory tract infections in infants presenting at the hospital. | ninety infants less than 1 year of age with pneumonia and 43 control infants were investigated for viral and chlamydial infection with the use of culture and serology and for bacterial infection with the use of blood cultures, lung aspirates, antibody assays and antigen detection procedures. one or more potential pathogens were identified in 62 (69%) cases with pneumonia and in 12 (28%) controls. infection by respiratory viruses was identified in 42 (49%) cases and in 8 (19%) controls. respirato ... | 1991 | 1848364 |
| nucleotide and derived amino acid sequences of the major porin of comamonas acidovorans and comparison of porin primary structures. | the dna sequence of the gene which codes for the major outer membrane porin (omp32) of comamonas acidovorans has been determined. the structural gene encodes a precursor consisting of 351 amino acid residues with a signal peptide of 19 amino acid residues. comparisons with amino acid sequences of outer membrane proteins and porins from several other members of the class proteobacteria and of the chlamydia trachomatis porin and the neurospora crassa mitochondrial porin revealed a motif of eight r ... | 1991 | 1848840 |
| clinical aspects of pelvic inflammatory disease. | pelvic inflammatory disease is caused by spread of organisms from the vagina and cervix to the upper genital tract. the patient may have a life-threatening illness or may remain asymptomatic. the disease may be caused by sexually or nonsexually transmitted organisms. polymicrobial infections are the rule. therapy requires antibiotic agents with broad coverage of aerobic and anaerobic organisms, including chlamydia trachomatis and neisseria gonorrhoeae. | 1991 | 1850572 |
| [chlamydia trachomatis infection in women and the use of oral contraceptives]. | | 1991 | 1852232 |
| the lack of utility of the latex agglutination test for detection of candida antigen in the diagnosis of systemic candidiasis. | the ramco latex agglutination test in the diagnosis of systemic candidiasis was utilized for 11 serum samples from 10 patients with systemic candidiasis, 21 serum samples from patients colonized with candida species, and 20 control serum samples from patients with stable medical problems and no evidence of candida albicans infection. this study was double-blind and the results of the latex agglutination test did not influence the decision for antifungal therapy. nine of 10 patients with systemic ... | 1991 | 1852278 |
| microbiological evaluation of female patients in std clinics. | a total of 215 women patients attending the std clinic were evaluated in an attempt to isolate the different microorganisms in sexually transmitted diseases (std). mycoplasmas (30.22%), candida species (20.00%), trichomonas vaginalis (wet mount study; 15.81%), beta haemolytic streptococci (13.48%), neisseria gonorrhoeae (9.30%), staphylococcus aureus (13.95%), inclusion bodies of chlamydia trachomatis (11.60%) and gram negative organisms (9.30%) were isolated from these patients. sera of all pat ... | 1991 | 1855826 |
| neutralization of chlamydia trachomatis: kinetics and stoichiometry. | monoclonal antibodies to the major outer membrane protein of chlamydia trachomatis were used to neutralize c. trachomatis infectivity in hela 229 cells and to determine the kinetics and stoichiometry of the reaction. in vitro neutralization of c. trachomatis infectivity proceeded as a first-order reaction and required an activation energy of approximately 20 kcal/mol (ca. 84 kj/mol). the rate of neutralization was linear with respect to antibody concentration and reaction temperature. the effici ... | 1991 | 1855982 |
| cloning and sequence analysis of the major outer membrane protein gene of chlamydia psittaci 6bc. | the gene encoding the major outer membrane protein (momp) of the psittacine chlamydia psittaci strain 6bc was cloned and sequenced. n-terminal protein sequencing of the mature momp indicated that it is posttranslationally processed at a site identical to the site previously identified in the momp of chlamydia trachomatis l2. the nucleotide sequence of the c. psittaci 6bc momp gene was found to be 67 to 68% identical to those of human c. trachomatis strains, 73% identical to that of chlamydia pne ... | 1991 | 1856001 |
| [pneumocystis carinii pneumonia: possible applications of pentamidine aerosol in hematology, oncology and transplantation medicine]. | | 1991 | 1856068 |
| detection of chlamydia trachomatis dna in patients with non-gonococcal urethritis using the polymerase chain reaction. | a practical protocol using the polymerase chain reaction (pcr) was designed for detecting chlamydia trachomatis in clinical samples. dna was extracted from material collected on urethral swabs and used as substrate for the pcr. the target was a 600 basepair dna segment of the multicopy plasmid that is common to all strains of the bacterium. negative samples were checked for loss of dna or presence of polymerase inhibitors by a second pcr, targeted to a conserved segment of the human genome. the ... | 1991 | 1856288 |
| clinical manifestations of perinatal sexually transmitted diseases. | | 1991 | 1857103 |
| rectal lymphogranuloma venereum in a bisexual patient. | rectal lymphogranuloma venereum (lgv) due to chlamydia trachomatis (ct) serotype l2 is reported. lgv was isolated from the lymph node biopsy of a bisexual male and was cultured on mccoy cycloheximide treated-cells. serological tests such as the micro-immunofluorescent test and the indirect immunoperoxidase assay for chlamydia trachomatis done on the patient's serum were positive with a titer of 1/200 and 1/256 respectively. smears taken from the rectal discharge were stained by the direct immuno ... | 1991 | 1857266 |
| impairment of amino-induced renal hyperemia in pancreatectomized dogs. | | 1991 | 1857476 |
| regulation of calcitonin gene-related peptide mrna expression in the hearts of spontaneously hypertensive rats by testosterone. | previous studies have demonstrated the existence of calcitonin gene-related peptide (cgrp)-immunoreactive nerve fibres and nerve cell bodies in the rat heart. using polymerase chain reaction we have investigated whether cgrp messenger rna (mrna) could be detected in heart tissue of spontaneously hypertensive rats, and whether cgrp-mrna levels are affected by gonadectomy and testosterone substitution. two weeks after castration cgrp-mrna levels decreased to 65.2 +/- 6.4% of control values, wherea ... | 1991 | 1857562 |
| regulation of calcitonin gene-related peptide mrna expression in the hearts of spontaneously hypertensive rats by testosterone. | previous studies have demonstrated the existence of calcitonin gene-related peptide (cgrp)-immunoreactive nerve fibres and nerve cell bodies in the rat heart. using polymerase chain reaction we have investigated whether cgrp messenger rna (mrna) could be detected in heart tissue of spontaneously hypertensive rats, and whether cgrp-mrna levels are affected by gonadectomy and testosterone substitution. two weeks after castration cgrp-mrna levels decreased to 65.2 +/- 6.4% of control values, wherea ... | 1991 | 1857562 |
| sexual experience, abortion and sexually transmitted diseases among young women at a contraceptive clinic. | all visitors who during one year visited a contraceptive clinic in the solna district of stockholm county and were below the age of 26 were asked to fill in a questionnaire and offered a test for c. trachomatis. three hundred and six women (97%) participated. ninety percent had at some time used oral contraceptives and 91% had experience of condom use. one-fifth of the whole group had undergone one or more legal abortions, and one fourth had experience of a sexually transmitted disease (std). th ... | 1991 | 1858499 |
| myocardial contractile actions of endothelin-1 in rat and rabbit papillary muscles. role of endocardial endothelium. | the actions of endothelin on the contractile and twitch configuration characteristics of rat and rabbit papillary muscles were evaluated before and after endocardial endothelium removal. in rabbit papillary muscles, endothelin produced a dose-dependent (10(-11) to 10(-7) m) increase in tension (t) (from 2.7 +/- 0.2 to 8.9 +/- 0.7 g/mm2, p less than 0.01), dt/dt, and vmax (from 1.14 +/- 0.03 to 3.0 +/- 0.13 lmax/sec, p less than 0.01), a decrease in time to peak tension (from 251 +/- 9 to 216 +/- ... | 1991 | 1860176 |