| delivery dependent oxygen consumption in patients with septic shock: daily variations, relationship with outcome and the sick-euthyroid syndrome. | delivery dependent oxygen consumption (ddoc) is observed in patients with sepsis and vital organ dysfunction, and has been related to outcome. similarly the sick-euthyroid syndrome is associated with a high mortality. we examined the daily variations of ddoc and its relation to hormonal changes, particularly those of the thyroid. in 22 patients, 14 with septic shock and 8 post-operative controls, oxygen delivery was increased by increasing cardiac output with vasodilation by phentolamine, during ... | 1991 | 1744322 |
| the 31 kd major allergen, alt a i1563, of alternaria alternata. | a component of alternaria extract, previously identified as the major allergen, alt a i1563, was purified to homogeneity from alternaria mycelium by means of acetone precipitation and ion-exchange chromatography. the homogeneity of alt a i1563 was assessed by one single band after sodium dodecyl sulfate-polyacrylamide gel electrophoresis, by one single radiolabeled band after transfer to nitrocellulose, and by one single peak after size-exclusion chromatography by high-performance liquid chromat ... | 1991 | 1744361 |
| hormones and pancreatic cancer. | several polypeptide hormones have been demonstrated to stimulate or inhibit cell division in the cells of the pancreas. therefore, receptors for these hormones have been sought in pancreatic carcinomas, and several examples have been reported. in some instances, stimulation of tumor growth by the corresponding peptide has been demonstrated or growth was blocked by a receptor antagonist. receptors and binding proteins for steroid hormones also have been reported in carcinomas of the pancreas. in ... | 1991 | 1744451 |
| endothelin-1 induces inositol phosphate production in rat pregnant myometrium. | | 1991 | 1744468 |
| bacterial lipopolysaccharides alter human endothelial cell morphology in vitro independent of cytokine secretion. | lipopolysaccharides derived from six bacterial species were found to alter human endothelial cell morphology in vitro in a species-dependent and dose-dependent manner. lipopolysaccharides derived from salmonella enteritidis (selps) induced the strongest response, whereas lipopolysaccharides from vibrio cholerae produced no effect. cell-shape changes induced by selps (10 micrograms/ml) were noticeable by 24 hours and reached a maximum by 72 hours, thus paralleling the effects produced by the reco ... | 1991 | 1744505 |
| upper extremity wound management: intermediate infections. | a careful history and physical examination, including information regarding position of the hand at the time of injury, presence of other disease, handedness, age, and occupation, are all essential to evaluate hand infection. if surgery is anticipated, antibiotics should generally be withheld until operative cultures are obtained. infections secondary to human bite injuries are common and are usually caused by a blow of the fist to the mouth. these injuries can produce crippling sequelae, must b ... | 1991 | 1744513 |
| limiting dilution analysis of cytotoxic t lymphocytes to human immunodeficiency virus gag antigens in infected persons: in vitro quantitation of effector cell populations with p17 and p24 specificities. | the presence of cytotoxic t lymphocytes (ctl) to the gag antigens of human immunodeficiency virus (hiv) has been described in infected populations. we found that the majority of this immune response as measured in bulk ctl assays of unstimulated peripheral blood mononuclear cells (pbmc) is directed against the p24 component of the p55 gag precursor protein. using limiting dilution analysis of this effector cell population we confirm that the majority of activated gag-specific ctl circulating in ... | 1991 | 1744586 |
| superficial thrombophlebitis of the anterior chest wall (mondor's disease). | | 1991 | 1744609 |
| vacuous jaw movements and feeding deficits in rats with ventrolateral striatal dopamine depletion: possible relation to parkinsonian symptoms. | a series of experiments examined the effects of regional dopamine depletions produced by intrastriatal injections of 6-hydroxydopamine on voluntary and involuntary movements in rats. depletion of dopamine in the ventrolateral striatum produced a substantial decrease in food intake, from which the animals recovered. rats with dopamine depletions in anteroventromedial or dorsolateral striatum did not have significant feeding deficits. rats with ventrolateral dopamine depletions showed no deficits ... | 1991 | 1744692 |
| clinical evaluation of a scintigraphic method for diagnosing inflammations/infections using indium-111-labeled nonspecific human igg. | this study was undertaken as part of a phase ii study to assess the sensitivity and safety of 111in-dtpa-human igg, an imaging agent for the detection of inflammations and/or infections. forty patients with infection/inflammation on the basis of clinical findings, microbiologic results, and/or the basis of results from other imaging modalities were studied. for evaluation of sensitivity, whole-body images were obtained at 6-12 hr (early) and 20-28 hr (delayed) postinjection and occasionally at 4 ... | 1991 | 1744707 |
| peripheral spatial vision: limits imposed by optics, photoreceptors, and receptor pooling. | we examined the contribution of optical and photoreceptor properties as well as receptor pooling to eccentricity-dependent variations in spatial vision by comparing the performance of ideal observers with that of human observers. we measured contrast sensitivity functions in human observers and calculated such functions in ideal observers for retinal eccentricities of 0-40 deg. comparisons of human and ideal performance in a variety of tasks reveal that many aspects of the variation in spatial v ... | 1991 | 1744774 |
| birefringence of the human crystalline lens in vivo. | we studied aspects of the birefringence in the human crystalline lens. with the use of mueller-matrix ellipsometry in vivo on both accommodated and unaccommodated eyes, we found no difference between the associated retardations. we calculated form birefringence of the lens by interpreting the membranes of the lens fiber cells as wiener bodies. the resulting retardation for a light beam that passes the lens as in the experiments exceeds by far the measured total retardation. we conclude that form ... | 1991 | 1744775 |
| [effects of heavy metal ions in endocochlear dc potential and cochlear microphonics in the guinea pig--the influence of calcium on the cochlea]. | endocochlear dc potential (ep) and cochlear microphonics (cm) in the guinea pig under the influence of the divalent heavy metal cations of manganese, nickel, cobalt and cadmium, and the trivalent cation of lanthanum were investigated. the area from the scala tympani to the scala vestibuli was perfused with control and test solutions. cm decreased gradually to 50-80%, but ep showed no change after perfusion with a solution containing 1 mm of metal ions. at a concentration of 10 mm, ep decreased f ... | 1991 | 1744784 |
| mhc antigen expression in human oral squamous carcinoma cell lines. | this study quantified the constitutive and interferon-gamma (ifn-gamma) stimulated expression of mhc class i (hla-abc and beta 2 microglobulin) and class ii antigens (hla-dr, -dp, -dq) on normal and malignant oral keratinocytes using radioimmunoassay and immunocytochemical techniques. normal keratinocytes and three of four malignant cell lines (h103, h157, h314) expressed mhc class i antigens constitutively; ifn-gamma increased mhc class i expression with significant changes in normals, h157 and ... | 1991 | 1744799 |
| rape and sexually transmitted diseases: patterns of referral and incidence in a department of genitourinary medicine. | a retrospective study was carried out of all women attending a department of genitourinary medicine over a 3-year period. note was taken of referring source, presenting symptoms, infection detected at std screening and follow-up attendance. comparison was also made between the number of women referred by the police surgeon and the number who actually attended. we observed an overall incidence of std of 35% and noted that many infections had a similar prevalence to that of our normal clinic popul ... | 1991 | 1744870 |
| pharmacokinetics of tinidazole in dogs and cats. | pharmacokinetics of tinidazole in dogs and cats after single intravenous (15 mg/kg) and oral doses (15 mg/kg or 30 mg/kg) were studied in a randomized crossover study. tinidazole was completely absorbed at both oral dose levels in cats and dogs. peak tinidazole concentration in plasma was 17.8 micrograms/ml in dogs and 22.5 micrograms/ml in cats after 15 mg/kg p.o. the oral dose of 30 mg/kg resulted in peak levels of 37.9 micrograms/ml in dogs and 33.6 micrograms/ml in cats. the apparent total p ... | 1991 | 1744934 |
| red cell trapping and postischemic renal blood flow. differences between the cortex, outer and inner medulla. | the distribution of blood flow in the rat kidney after 60 minutes of renal ischemia was studied by single-fiber laser-doppler flowmetry. blood flow in superficial cortex and inner medulla was measured with a probe directed towards the kidney surface and exposed papilla, respectively. outer medullary blood flow was measured with a probe introduced through the renal core. after ischemia the blood flow decreased to 60% of the preischemic value (p less than 0.01) in superficial cortex and to 16% (p ... | 1991 | 1745011 |
| study of the management of chlamydial cervicitis in general practice. | the role of the general practitioner in the detection and management of chlamydia trachomatis infections of the cervix is uncertain. the management by the primary care team of women presenting with lower genital tract symptoms has therefore been studied in one suburban practice. of 386 women presenting with lower genital tract symptoms over the two year study period 25 (6%) had a positive cervical microtrak (syva) test for chlamydia trachomatis. twenty four of these chlamydia positive patients w ... | 1991 | 1747265 |
| comparison of different culture media for isolation of chlamydia trachomatis by cell culture on hela cells. | the isolation yield and number of chlamydia trachomatis inclusions was compared using deae-dextran pretreated hela cells cultured in four different media: eagle's minimal essential medium (emem) with and without cycloheximide and dulbecco's modification of emem (dmem) with and without cycloheximide. using dmem without cycloheximide the number of inclusions was significantly higher than using emem without cycloheximide. in addition, the size of the inclusions was greatly enhanced. use of dmem or ... | 1991 | 1748120 |
| detection of chlamydia trachomatis by direct immunofluorescence improved by centrifugation of specimens. | during a study of women with laparoscopically investigated pelvic pain, genital tract specimens were examined for chlamydia trachomatis using a direct fluorescent antibody (dfa) technique (microtrak, syva) and culture. some smears, particularly those from the upper genital tract, contained an inadequate number of cells when examined by the dfa technique and many cell monolayers were destroyed by the specimens. to obtain results for such samples, or to confirm the original dfa result, additional ... | 1991 | 1748121 |
| evaluation of a chemiluminometric immunoassay and a direct immunofluorescence test for detecting chlamydia trachomatis in urogenital specimens. | a new chemiluminometric immunoassay (magic lite) and a direct immunofluorescence technique (microtrak) were compared with cell culture in the diagnosis of chlamydia trachomatis infections. both assays were evaluated using 260 genital specimens (89 men, 171 women), 26 of which were culture positive. the respective sensitivity of the chemiluminometric immunoassay and immunofluorescence technique was 88.5% and 84.6%, the specificity 97% and 99.6%, the positive predictive value 76.7% and 95.6%, and ... | 1991 | 1748122 |
| drugs for sexually transmitted diseases. | | 1991 | 1749363 |
| a chlamydial plasmid is differentially transcribed during the life cycle of chlamydia trachomatis. | a 7.5-kb cryptic plasmid is found in all serotypes of the obligate intracellular parasite, chlamydia trachomatis. although at least nine open reading frames are apparent from sequence analysis of plasmid dna, only a small region of approximately 500 bp has been consistently shown to be transcriptionally active by northern blot analysis. in this study, transcription was analyzed using a host-free system in which rna synthesized by chlamydiae isolated from host cells was hybridized to different re ... | 1991 | 1749817 |
| rna-directed molecular hybridization screening: evidence for inapparent chlamydial infection. | both clinical and epidemiological data suggest that inapparent infection by chlamydia trachomatis occurs in humans. to confirm and study such infections, we developed a hybridization screening system directed toward chlamydial ribosomal rna (rrna). six restriction endonuclease fragments derived from the cloned rrna operon of chlamydial serovar l2(434) were tested as hybridization screening probes, but only one fragment encoding the 5' portion of the 16s rrna gene plus some upstream flanking sequ ... | 1991 | 1750444 |
| [prevention of neonatal conjunctivitis. a comparative clinical and bacteriologic study of 2 eyedrops: silver nitrate and oxytetracycline chlorhydrate]. | this study carried out at the saint-germain-en-laye hospital maternity ward included all the neonates delivered between february and september 1989 who exhibited no abnormal manifestations during their stay in the ward, except for ocular symptoms in some subjects. nine hundred neonates were enrolled. each day, one of two eyedrop preparations for the prevention of neonatal ocular infections was selected at random. investigators were blinded to the preparation used. study subjects were evaluated t ... | 1991 | 1750748 |
| exogenous opioids increase plasma prolactin in holstein calves primarily via a dopaminergic mechanism. | a study was conducted to determine whether exogenous opioids increase prolactin (prl) secretion in holstein heifer calves via a dopaminergic mechanism. twenty-four holstein heifer calves ranging in age from 5 to 7 mo were assigned to one of four treatment groups (six/treatment): 1) injection of saline (sal); 2) injection of a synthetic enkephalin (d-ala2, n-me-phe4, met(o)5-ol enkephalin; damme); 3) injection of damme after pretreatment with the long-acting dopamine agonist 2-bromo-alpha-ergocry ... | 1991 | 1752829 |
| risk factors for spontaneous abortion: a case-control study in france. | a case-control study was conducted in seven maternity hospitals in the paris area in 1988 to evaluate the role of several risk factors in spontaneous abortion. a total of 279 cases and 279 controls were compared for socio-demographic characteristics, reproductive history and for conditions of conception. prior fetal losses [odds ratio (or) = 2.30 for n greater than or equal to 2; 95% confidence interval (ci) = 1.17-4.61] and maternal age at pregnancy (greater than 30 years) appeared to be major ... | 1991 | 1752937 |
| pulmonary nocardiosis concurrent with pneumocystis carinii pneumonia in two patients undergoing immunosuppressive therapy. | two cases with concomitant pulmonary nocardiosis and pneumocystis carinii pneumonia are described. the first patient developed pneumonia 3 months after heart transplantation while undergoing standard immunosuppressive therapy with cyclosporin, azathioprine and prednisone. the second patient was treated with chemotherapy and subsequent radiotherapy of the mediastinum for a malignant epithelial tumour. he also received prednisone for paraneoplastic dermatomyositis. chest x-rays of both patients sh ... | 1991 | 1753118 |
| molecular characterization of new group a streptococcal bacteriophages containing the gene for streptococcal erythrogenic toxin a (spea). | bacteriophage t12 is the prototype phage carrying the streptococcal erythrogenic toxin a (spea) gene. to examine more closely the phages involved in lysogenic conversion, we examined 300 group a streptococcal strains, and identified and isolated two new phages that carry the spea gene. the molecular sizes of these phage genomes were between 32 and 40 kb, similar to that of phage t12 (35 kb). however, as ascertained by restriction analysis, the physical maps of the new phage genomes were differen ... | 1991 | 1753942 |
| performance of a nonisotopic dna probe for detection of chlamydia trachomatis in urogenital specimens. | the gen-probe pace 2 assay (gp), a nonisotopic dna probe, was evaluated by using cell culture as the method of reference. specimens were collected from 260 men and 482 women visiting the outpatient department for sexually transmitted diseases at the university hospital in rotterdam, the netherlands. the prevalences of chlamydia culture-positive men and women were 13.2 and 8.6%, respectively. sensitivity values for the male and female patients were 70.6 and 92.7%, respectively. specificity values ... | 1991 | 1757533 |
| diagnosis of chlamydia trachomatis cervical infection by detection of amplified dna with an enzyme immunoassay. | | 1991 | 1757574 |
| [inhibition of the cysteamine-induced gastric ulcer by the sympathoadrenal medullary system in spontaneously hypertensive rats (shr) and normotensive wistar-kyoto rats (wky)]. | we previously reported that cysteamine induces severe gastric ulcers in wky, but very mild in shr. the aim of this study is to elucidate the role on the sympathoadrenal medullary system in the pathogenesis of the cysteamine-induced gastric ulcer. catecholamine (ca) contents in the stomach and adrenal gland were significantly higher in the non-treated shr than in the non-treated wky, suggesting that the sympathetic nervous system is more facilitated in shr. cysteamine decreased the noradrenaline ... | 1991 | 1758079 |
| [etiology of left ventricular rupture following mitral valve replacement]. | two uncommon cases of left ventricular rupture that occurred during cardiac surgery were treated successfully. these cases may be useful in understanding the etiology of common left ventricular rupture following mitral valve replacement. one case occurred during coronary bypass surgery. the myocardium which is already abnormal seems to be weak to trauma such as bending, traction and torsion. in the other case, who underwent mitral valve replacement with preservation of the posterior leaflet with ... | 1991 | 1758117 |
| stress-induced gastric lesions and the synthesis of prostaglandins and leukotrienes. | prostaglandin e2 renders the mucosa of the gi tract more resistant to acute injury by aspirin, acid, bile salts, and stress, whereas leukotrienes promote inflammation and retard healing of ulcers. the relationship of stress ulcer formation to changes in the activities of prostaglandin synthetase and lipoxygenase was evaluated in this study. after a 24 hr fast, 10 rats were stressed by the cold-restraint method for 4 hr and 10 rats were not stressed. rats were terminated, stomachs were excised, t ... | 1991 | 1758169 |
| the vo2max of recreational athletes before and after pregnancy. | this study was designed to test the hypothesis that pregnancy has an added training effect (increases "absolute" vo2max) in well-conditioned, recreational athletes. vo2max was measured serially in 20 nonpregnant recreational athletes who maintained their exercise within +/- 10% of initial levels over a 15-month period and 20 similar women who conceived and continued exercise at a reduced level during pregnancy with a return to within 20% of initial levels by 12 wk postpartum. initially the two g ... | 1991 | 1758289 |
| effect of cd2+ on phosphate uptake by cadmium-resistant and cadmium-sensitive staphylococcus aureus. | the effect of cd2+ on phosphate (pi) uptake was investigated in the growing cells of cd(2+)-resistant staphylococcus aureus 1781or and cd(2+)-sensitive s. aureus 17810s. inhibitor and ionophore studies showed that 32pi uptake in the two strains occurred via the pi porter down ph gradient (delta ph) generated by the respiratory chain. cd2+ inhibited 32pi uptake in the cadmium-sensitive strain 1781os at all concentrations used (10 microm-1 mm). in strain 1781or, possessing the plasmid-coded cd2+ e ... | 1991 | 1758309 |
| iraq-occupied kuwait: the health care situation. conclusions. physicians for human rights (denmark and usa). | | 1991 | 1758350 |
| [the interferon status in helminthiases. 3. opisthorchiasis]. | in 23 patients with opisthorchis felineus infection, 4 in subacute and 19 in chronic stages of the disease (7 subclinical, 8 with cholepathy and 4 with gastroduodenitis) the blood levels of alpha-interferon (if-alpha) and if-gamma were measured using vesical stomatitis virus culture. in patients with subacute opisthorchiasis the blood contents of if-alpha and if-gamma were the same as in control-30 practically healthy people (640-1280, log2 9.73 +/- 0.19, and 128-256, log2 7.75 +/- 0.25 against ... | 1991 | 1758354 |
| longitudinal study of the microbiology of endemic trachoma. | a longitudinal study of trachoma in 100 members of nine tanzanian families was conducted to assess the sources of variation in the laboratory identification of trachoma and the changes that might occur over time. multiple conjunctival swabs were collected every 3 months for 1 year and examined by direct fluorescent-antibody cytology (dfa), enzyme immunoassay, or microimmunofluorescence serology for tear antichlamydial antibodies. dfa specimens collected 5 min apart had a discordance rate of 10% ... | 1991 | 1761679 |
| [detection of chlamydia trachomatis by polymerase chain reaction]. | a polymerase chain reaction (pcr) procedure was developed for detection of chlamydia trachomatis. two oligonucleotide primers based on sequences within the major outer membrane protein gene from c. trachomatis serovar l2 were used. a single dna fragment was amplified, when c. trachomatis dna was template for the pcr. no amplified product was detected in chlamydia psittaci dna, chlamydia pneumoniae dna or other bacterial dnas. the amplified dna fragment was detected, when dna of greater than or e ... | 1991 | 1761897 |
| [significance of determination of the blood antibody level in chlamydia trachomatis infection of the uterine cervix]. | kits for measurement of c. trachomatis specific antibody are not commercially available, but of the significance of determination of the serum antibody level has not been clarified. in this study, we first determined the c. trachomatis antibody level in patients considered to be free of c. trachomatis infection. then, we evaluated the relationship between the presence or absence of antigen with the antibody level in patients at our department who were available for antigen examination. anti-c. t ... | 1991 | 1762201 |
| [urogenital chlamydia trachomatis infections]. | urogenital chlamydia trachomatis infections are the most common sexually transmitted diseases which have shown a significant increase in the last few years. this organism presents a major health problem in many countries. it is recognized as the cause of both complicated and uncomplicated infections in men, women and infants passing through an infected birth canal. tissue culture is accepted as the gold standard in the diagnosis of chlamydial infections. but, since this technique is expensive an ... | 1991 | 1762596 |
| a comparison of d1 receptor binding and mrna in rat brain using receptor autoradiographic and in situ hybridization techniques. | d1, a subtype of the dopamine receptors, is widely distributed in the nervous system and has been shown to be positively coupled to adenylate cyclase. using a combination of in vitro receptor autoradiographic and in situ hybridization techniques, the present study examines the co-distribution of d1 receptor binding sites and d1 receptor messenger rna in adjacent rat brain sections. d1 receptor binding sites were labeled using the selective antagonist [3h]sch23390 (4.6 nm) in the presence of 1 mi ... | 1991 | 1762683 |
| contribution of the known subcellular effects of anesthetics to their negative inotropic effect in intact myocardium. | the results of these studies suggest that halothane, in addition to its effect of reducing the trans-sarcolemmal ca2+ influx, has a direct effect on the function of the sarcoplasmic reticulum by making the organelle leaky to ca2+ and reducing the amount of ca2+ stored. isoflurane, on the other hand, does not appear to make the sarcoplasmic reticulum leaky to ca2+. in fact, our observations suggest that isoflurane makes the sarcoplasmic reticulum less leaky to ca2+. halothane may enhance the redu ... | 1991 | 1763690 |
| effects of volatile anesthetics on the intracellular ca2+ concentration in cardiac muscle cells. | | 1991 | 1763691 |
| recent developments in the treatment of sexually transmitted diseases. | recent changes in the treatment of sexually transmitted diseases include recognition of penicillin-resistant neisseria gonorrhoeae, identification of chlamydia trachomatis as the leading cause of bacterial genital infection in the united states, and the realization that the urethritis syndrome is often associated with multiple pathogens. there is currently no monotherapy that eradicates all std pathogens. the role of fluoroquinolones in the treatment of stds is still evolving. the investigationa ... | 1991 | 1767802 |
| analysis of high psa n-cam expression during mammalian spinal cord and peripheral nervous system development. | using a monoclonal antibody that recognizes specifically a high polysialylated form of n-cam (high psa n-cam), the temporal and spatial expression of this molecule was studied in developing spinal cord and neural crest derivatives of mouse truncal region. temporal expression was analyzed on immunoblots of spinal cord and dorsal root ganglia (drgs) extracts microdissected at different developmental stages. analysis of the ratio of high psa n-cam to total n-cam indicated that sialylation and desia ... | 1991 | 1769342 |
| attenuation of insulin-induced drinking by beta-adrenoceptor antagonists. | insulin-induced drinking (iid) in male wistar rats, evoked by administering 5 u/kg of crystalline porcine insulin i.p., was significantly decreased by propranolol (0.1 and 0.5 mg/kg s.c.) after 1 and 2 h. the blood glucose of rats treated with a much higher dose of propranolol (10 mg/kg body weight) and insulin did not differ from that of rats treated solely with insulin after 30 and 120 min. atenolol (0.5 mg/kg s.c.) caused a reduction in iid after 1 and 2h. butoxamine (1 mg/kg s.c.) also reduc ... | 1991 | 1769367 |
| [the value of symptoms and clinical findings in cervical chlamydia trachomatis infection]. | the frequency of symptoms in the case histories of 68 female patients with and of 313 without chlamydia trachomatis infections was investigated. dysuria, vaginal discharge and/or burning/itching in the genital region were reported by 50% (n = 34) of chlamydia-positive women and 53.7% (n = 168) of chlamydia-negative women. clinical investigation of the chlamydia-positive patients revealed discharge in 83.8% (n = 57), contact bleeding in 38.3% (n = 26) and ectopia in 27.9% (n = 19). the correspond ... | 1991 | 1769832 |
| perinatal androgen administration and the maintenance of sexually dimorphic and nondimorphic lumbosacral motor neuron groups in female albino swiss rats. | motor neuron groups in the l5-s1 region of the spinal cord were examined in male and female albino swiss rats and in females treated with 5 alpha-dihydrotestosterone benzoate (dhtb) either prenatally or postnatally. the spinal nucleus of bulbocavernosus (snb) contained some five times more neurons in males than females. perinatal dhtb treatment to females increased snb numbers; prenatal treatment was less effective than postnatal. within the same part of the cord, males possessed more motor neur ... | 1991 | 1769898 |
| inhibitors of sterol synthesis. characterization of trimethylsilyl dienol ethers of 3 beta-hydroxy-5 alpha-cholest-8(14)-en-15-one. applications in the analysis of mitochondrial metabolites of the 15-ketosterol by gas chromatography-mass spectrometry. | derivatization of delta 8(14)-15-ketosterols as bis-tms dienol ethers facilitates their analysis by gas chromatography-mass spectrometry (gc-ms). conditions are presented for the preparation of each of the three possible bis-tms dienol ethers of 3 beta-hydroxy-5 alpha-cholest-8(14)-en-15-one (1), a potent regulator of cholesterol metabolism. treatment of 1 with n,o-bis(tri-methylsilyl)-trifluoroacetamide (bstfa) and pyridine for 20 h at 100 degrees c produced primarily 3 beta,15-bis(trimethylsil ... | 1991 | 1770319 |
| efficacy of three pyrethroids against leptotrombidium fletcheri (acari: trombiculidae) infected and noninfected with scrub typhus. | toxicities of three pyrethroids, d-phenothrin, decamethrin, and permethrin, were evaluated in the laboratory against leptotrombidium fletcheri (womersley & heaslip). the susceptibilities between populations of the species infected and noninfected with scrub typhus were investigated. the three pesticides exhibited different toxicities to the chiggers. d- phenothrin was the most toxic, followed by decamethrin, then permethrin. there were no significant differences between susceptibilities of the i ... | 1991 | 1770512 |
| cuticular hydrocarbon geographic variation among seven north american populations of aedes albopictus (diptera: culicidae). | cuticular hydrocarbons were used to differentiate among the following north american populations of aedes albopictus: chicago, ill.; milford, del.; jacksonville, fla.; san antonio, tex.; new orleans, la.; houston, tex.; and lexington, ky. forty cuticular hydrocarbon peaks were identified from each population using gas chromatography electron impact-mass spectrometry: 16 n-alkanes, 21 monomethylalkanes, and 3 dimethylalkanes. fourteen peaks occurred at equivalent chain lengths greater than 38.65 ... | 1991 | 1770522 |
| antibiotic treatment of pharyngitis. | pharyngitis is one of the most common medical problems and ranges in severity from minor to fatal. the treatment regimens presented have been standardized for the various pathogens on the basis of clinical response and pathogen eradication. the current major challenge is the rapid recognition and diagnosis of streptococcal infection and clarification of the etiologic role of several nonstreptococcal pathogens. | 1991 | 1771305 |
| epidemiologic characteristics of two different populations of women with chlamydia trachomatis infection and their male partners. | two groups of women under the age of 26 with chlamydial infection (a-asymptomatic women from a screening program, b-traditional std clinic patients) and their partners were compared with respect to history of std, sexual behavior, contraception, and indices for income and social welfare expense. the mean number of partners was 1.3 in group a and 1.8 in group b. women in group b, in spite of more frequent condom use, demonstrated greater risk behavior both with respect to std and to unwanted preg ... | 1991 | 1771473 |
| neisseria meningitidis in specimens from urogenital sites. is increased awareness necessary? | neisseria meningitidis serogroups b type 2 and y were isolated from urogenital specimens from three heterosexual patients. the first patient was a young man with the clinical signs and microscopic findings of a typical gonococcal urethritis. the second was a middle-aged woman with cervicitis, in whom neither neisseria gonorrhoeae nor chlamydia trachomatis were demonstrated by culture. in the third patient, a young woman, n. meningitidis was associated with cervicitis, acute salpingitis, and peri ... | 1991 | 1771476 |
| a retrospective study of efforts to diagnose infections by chlamydia trachomatis in a swedish county. | diagnostic work on the detection of chlamydia trachomatis infections in uppsala county, sweden, during the period from april 1985 to march 1991 was studied. the number of patients examined for c. trachomatis in this area increased annually by 6% to 15% during the study period, reaching 17,224 test occasions by the last 12-month period. a 40% decrease (from 1,412 to 852; or from 565 to 326 cases per 100,000 inhabitants) in diagnosed c. trachomatis infections was noted between the first and the la ... | 1991 | 1771477 |
| enterovirulence of enterotoxigenic bacteroides fragilis in gnotobiotic pigs. | a porcine isolate of enterotoxigenic bacteroides fragilis colonized the intestinal tract and caused watery, nonhemorrhagic diarrhea when given orally to 12, 1- to 2-day-old gnotobiotic pigs. diarrhea occurred 2 to 3 days post-inoculation and continued throughout the 4 to 6 day post-inoculation period. diarrheic pigs became mildly anorexic and dehydrated. they developed intestinal lesions characterized by swelling, vacuolation, and exfoliation of enterocytes, and crypt hyperplasia throughout the ... | 1991 | 1771741 |
| effect of perfluorodecalin on activities of hepatic phase ii xenobiotic biotransformation enzymes. | the activity of the hepatic phase ii enzymes of xenobiotic biotransformation after intravenous administration of perfluorodecalin emulsion to rats was measured. perfluorodecalin was found to increase the microsomal glutathione s-transferase and udp-glucuronosyltransferase activities 1.4- and 2.3-fold, respectively. the activity of sulphotransferase was decreased 2-fold. these results show that perfluorodecalin is an inducer of both the enzymes of cytochrome p-450-dependent monooxygenase system [ ... | 1991 | 1772431 |
| changes in the c.d. spectra of calf thymus dna induced by sequential polypeptides in trifluoroethanol solutions. part ii. | interactions between calf thymus dna and (l-arg-x-gly)n sequential polypeptides (where x = l-ala, val, leu, ile, nva, nle) in trifluoroethanol: water (40:60) solutions in the salt range of 0.12-0.5 m nacl, were studied using c.d. spectroscopy. it was found that dna tertiary structure (psi form) is modulated by the nature of the polypeptides (variation of x residue). the effect of the secondary structure of polypeptides on the formation of psi-dna was also analysed. unordered polypeptides destabi ... | 1991 | 1772827 |
| h-2 influence on the production of real but not of apparent h-2-specific antibodies induced by the association of syngeneic class i heavy chains with bovine beta 2-microglobulin. | immunogenic properties of class i molecules resulting from the association of mouse class i heavy chains with a xenogeneic beta 2-microglobulin (beta 2-m) were investigated by studying the antibody response of mice of injections to syngeneic con a lymphoblasts, induced in conditions allowing the replacement of endogenous beta 2-m by exogenously added bovine beta 2-m provided by fetal calf serum (fcs-con a blasts). lymphocytotoxic antibodies were regularly produced and according to their specific ... | 1991 | 1772881 |
| bacterial vaginosis and the vaginal leucocyte/epithelial cell ratio in women attending an outpatient gynaecology clinic. | in order to investigate the usefulness of vaginal leucocytosis as a sign of genital infection, vaginal wet smears were obtained from 230 consecutive non-pregnant women attending an outpatient gynaecology clinic. although 52 (22.6%) of the women were diagnosed as having bacterial vaginosis, none had symptoms of cervicitis or genital infection. of the patients with bacterial vaginosis 19 (36.5%) had vaginal leucocytosis compared to 37.1% of the women without bacterial vaginosis. in a further study ... | 1991 | 1773877 |
| chlamydia trachomatis infection among hungarian teenage girls. | the presence of chlamydia trachomatis antigen was examined in 20 sexually active teenage girls by enzyme immunoassay to prove chlamydial infection. seven of the study group showed evidence of c. trachomatis infection. the infection was more frequent in girls who used a non-barrier method for contraception and had more than four sexual partners previously. screening for and treatment of c. trachomatis infection in high-risk teenage girls is recommended. | 1991 | 1773879 |
| adverse foetal outcome in specific igm positive chlamydia trachomatis infection in pregnancy. | a study was undertaken to determine the adverse foetal outcome in antichlamydial igm positive asymptomatic pregnant females. an indirect immunoperoxidase assay was done to detect igm in 78 apparently normal asymptomatic pregnant women during the third trimester and follow up was done till delivery to study the effect of chlamydial infection on foetal outcome. a total of 28 (35.9%) women were positive for antichlamydial igm while only 3.33 per cent asymptomatic non-pregnant normal women (controls ... | 1991 | 1774093 |
| acute chlamydial salpingitis with ascites and adnexal mass simulating a malignant neoplasm. | chlamydia trachomatis is a well-known cause of acute and chronic salpingitis, accounting for approximately half of all cases of pelvic inflammatory disease. typically, patients with acute chlamydial salpingitis present with acute lower abdominal pain, tenderness on bimanual pelvic examination, or vaginal discharge. we describe a case of acute chlamydial salpingitis with marked ascites and an adnexal mass that simulated a malignant neoplasm. microscopically, a severe lymphofollicular salpingitis ... | 1991 | 1774110 |
| evaluation of the microbiology of chronic ethmoid sinusitis. | in a prospective study, patients with the diagnosis of chronic ethmoid sinusitis were evaluated microbiologically by using biopsy specimens of the ethmoid sinus mucosa. microbiology cultures were performed on 94 specimens from 59 patients. staphylococcus aureus and members of the family enterobacteriaceae were the most frequent classical pathogenic bacteria isolated. coagulase-negative staphylococci were the most common overall isolates. streptococcus pneumoniae and haemophilus influenzae were i ... | 1991 | 1774242 |
| use of a urine enzyme immunoassay as a diagnostic tool for chlamydia trachomatis urethritis in men. | we collected first-voided urine specimens from 659 males attending a sexually transmitted disease clinic and performed both enzyme immunoassay (eia) for detection of chlamydial antigen and leukocyte esterase testing on these urine samples. the overall prevalence of chlamydial urethritis in the study population as determined by culture of urethral swabs was 11%. however, 46% of all men in the study had no symptoms of urethritis. compared with urethral cultures for chlamydiae, the urine eia had a ... | 1991 | 1774248 |
| comparison of the clearview chlamydia test, chlamydiazyme, and cell culture for detection of chlamydia trachomatis in women with a low prevalence of infection. | two antigen detection systems, clearview chlamydia (unipath ltd., bedford, united kingdom) and chlamydiazyme (abbott laboratories, north chicago, ill.), were compared with culture for the diagnosis of chlamydia infection in women attending gynecological clinics. chlamydia trachomatis was isolated from 43 (4.5%) of the 965 women tested. in comparison with tissue culture, the clearview chlamydia and chlamydiazyme tests had sensitivities of 79.0 and 74.4%, respectively, and both had a specificity o ... | 1991 | 1774342 |
| nasal polyposis: in search of a viral etiology using dna hybridization. | the etiology of nasal polyps is unknown. numerous theories have been proposed, with allergic and infectious causes predominant. a viral theory was postulated almost 40 years ago. in a pilot project, we studied 15 subjects comprising six controls and nine patients with nasal polyps. no known viruses were cultured from the nasal polyp tissue or nasal mucosal biopsies from the control subjects. dna hybridization for epstein-barr virus, herpes simplex virus, adenovirus type v, and chlamydia trachoma ... | 1991 | 1774797 |
| evaluation of vaginal microflora in patients infected with hiv. | hiv infection is thought to exacerbate the virulence of normal saprophytic vaginal microflora. we studied the vaginal ecosystem of hiv patients to detect the quantitative and qualitative variation of vaginal microorganisms. 15 patients (5 with aids and 10 with arc) were investigated. vaginal candidiasis was more frequent in this group than in the control groups. gardnerella was present in 60% of patients generally in association with anaerobic bacteria and mycoplasma. among anaerobia, bacteroide ... | 1991 | 1775091 |
| evaluation of diagnostic procedures in chlamydial eye infection. | sixty-six patients suffering from suspected chlamydial conjunctivitis or keratoconjunctivitis underwent direct examination and culture procedures on specimens from conjunctival swabs and corneal scraping for detection of chlamydia trachomatis. patient blood samples were also screened for the presence of antichlamydia igg and iga antibodies. eye positivity was found in 12 and 15% of patients by using culture isolation and direct examination, respectively. in 3 out of the 8 patients with culture-p ... | 1991 | 1775298 |
| development of an automatic machine for in situ hybridization and immunohistochemistry. | an instrument for the automation of in situ hybridization and immunohistochemistry has been developed. this machine is capable of analyzing 20 microscope glass slides via all of the steps required for colorimetric in situ hybridization or immunohistochemistry. the slides are placed specimen-side down on a specialized teflon slide-holder set in the reaction chamber of the machine. the system uses a unique type of capillary action between the slide and the holder. the holder has two small holes an ... | 1991 | 1776690 |
| the role of chlamydia trachomatis and neisseria gonorrhoeae in the aetiology of ectopic pregnancy in gabon. | to study the association between ectopic pregnancy and chlamydia trachomatis and neisseria gonorrhoeae. | 1991 | 1777459 |
| studies on hypotensive agents. synthesis of 1-substituted 3-(2-chlorophenyl)-6-ethoxycarbonyl-5,7-dimethyl-2,4(1h,3h)-quinazoline diones. | 3-(2-chlorophenyl)-6-ethoxycarbonyl-5,7-dimethyl-2,4(1h,3h)-quinazoline dione was newly prepared. 1-hydrogen atoms of the compound were variously substituted in order to test for their hypotensive activities on relaxing effects of the blood vessels. the compounds with 2-(1-pyrrolidinyl) ethyl, 2-(1-piperidinyl)ethyl, 3-(dimethylamino)propyl, and 3-(n-benzyl-n-methylamino)propyl moieties showed significant activity. the 2-(1-piperidinyl)ethyl compound possessed activity approximately 23 times mor ... | 1991 | 1777928 |
| urethral isolation of chlamydia trachomatis in women with urinary incontinence. | the overall prevalence of chlamydia trachomatis (ct) infection in 168 patients with urinary incontinence was 7.7%. however, in patients with urethral instability ct was found significantly more often than in those with stable urethra (p less than 0.001). clinical and urodynamic improvement followed the treatment with doxycycline in 8 out of 9 patients with urethral instability. according to our results urethral infection with ct may play an important role in the etiology of urge incontinence and ... | 1991 | 1778292 |
| rapid genotyping of the chlamydia trachomatis major outer membrane protein by the polymerase chain reaction. | twenty one chlamydia trachomatis reference strains and 40 clinical isolates belonging to the lymphogranuloma venerum (lgv) and trachoma biovars were genotyped by differential restriction mapping of the major-outer-membrane-protein gene (momp) obtained by the polymerase-chain reaction (pcr). alui digestion of the pcr product distinguishes eight momp-genotypes corresponding to 8 serovars. six additional enzymes (nlaiii, cfoi, ecori, hinfi, ddei and foki) further permit the discrimination of 10 mom ... | 1991 | 1778424 |
| treatment of chlamydial conjunctivitis in newborns and adults with erythromycin and roxithromycin. | a randomized single-blind study of the effects of erythromycin and roxithromycin on chlamydial conjunctivitis was performed on a group of patients, comprising 28 newborns and 27 adults. treatment used was either 200 mg of erythromycin ethylsuccinate or 50 mg of roxithromycin daily, divided into two doses for the neonatal group or for the adult group, 1000 mg of erythromycin stearate or 300 mg of roxithromycin daily divided into two doses. all patients were treated for ten days. clinically nine o ... | 1991 | 1778860 |
| n-acetylglutamate synthetase deficiency: clinical and laboratory observations. | two male siblings presented in the first 6 weeks of life with emesis, diarrhoea, metabolic acidosis and lethargy. a male sibling had previously died at 14 months of age from liver failure of unknown aetiology. both of the current cases had mild hyperammonaemia with normal orotic acid, organic acid and argininosuccinic acid levels. citrulline and arginine levels were normal or mildly decreased. one of the brothers was biopsied and had no detectable n-acetylglutamate synthetase activity and normal ... | 1991 | 1779615 |
| evaluation of a dna probe of plasmid origin for the detection of chlamydia trachomatis in cultures and clinical specimens. | this study evaluates five cryptic plasmid-derived dna probes in a 4-h slot-blot hybridization assay for the detection of chlamydia trachomatis in cultures and clinical specimens. the probes, consisting of either the entire cloned 7.5 kbp cryptic plasmid pse8 or one of four hin diii/eco ri fragments measuring 710, 1055, 710, and 500 bp, respectively, were labelled with photoprobe biotin. the probe was detected using a streptavidin-alkaline phosphatase conjugate followed by addition of bcip and nb ... | 1991 | 1779980 |
| screening for chlamydia trachomatis by the direct fluorescent antibody test in female navy recruits. | female navy recruits at risk for sexually transmitted diseases were screened for chlamydia trachomatis. the screening method was the direct fluorescent antibody (fa) test. the patients represented all areas of the u.s. average age was 20.4 years. patients listed past gynecological infections and current symptoms. the clinical findings of the provider at examination and the results of the direct fa test were correlated. there does not appear to be a reliable office method to predict which patient ... | 1991 | 1780069 |
| the laboratory diagnosis of chlamydia trachomatis. | | 1991 | 1781089 |
| characterisation of a cell line (lcc-18) from a cultured human neuroendocrine-differentiated colonic carcinoma. | a cell line (lcc-18) from a neuroendocrine colonic tumour was established. the tumour cells retained their endocrine characteristics through more than 100 passages and showed positive immunocytochemistry for synaptophysin, vasoactive intestinal polypeptide (vip) and glucagon. the culture medium also contained vip and glucagon, which indicates that mechanisms for release of some of the active peptides were preserved. transplantation of lcc-18 tumour cells into nude rats resulted in tumour formati ... | 1991 | 1782079 |
| choice of sexual partner according to rate of partner change and social class of the partners. | models for the spread of sexually transmitted diseases, including aids, rely on sexual partner mixing patterns in the population. from data acquired through partner notification for infection with chlamydia trachomatis among young people in gothenburg, sweden, it was possible to construct contact matrices for the variables 'rate of partner change' and 'social class' of the partners in pair formations. these matrices show a restricted mixing between these two variables for young heterosexual wome ... | 1991 | 1782233 |
| sexually transmitted diseases in prostitutes in moshi and arusha, northern tanzania. | sexually transmitted diseases are thought to be important in facilitating transmission of hiv in sub-saharan africa. this study reports the prevalence of several sexually transmitted diseases in 106 prostitutes in arusha and moshi northern tanzania. the seroprevalence of hiv was 73% compared with 3% for local blood donors. over half (51%) of the subjects had evidence of n. gonorrhoeae infection. seventy-four per cent had a positive tpha and 27% a positive rpr. of 47 subjects tested 12 (25%) had ... | 1991 | 1782234 |
| role of polyamines in gastroprotection induced by epidermal growth factor. | polyamines have been shown to stimulate cellular growth and differentiation, though their role in the prevention of acute gastric lesion induced by various noxious agents has been little studied. epidermal growth factor (egf) exhibits gastroprotective and ulcer healing properties due to its potent mitogenic and growth promoting action. this study was designed to compare the gastroprotective effects of spermine and egf against gastric damage induced by absolute ethanol, acidified aspirin and stre ... | 1991 | 1782416 |
| listeria in effluents from the food-processing industry. | there is general agreement that listeriosis has a significant impact on man as well as on animals. listeria monocytogenes has been isolated from the faeces of healthy human and animal carriers and from various environmental sources. l. monocytogenes is the pathogenic species most responsible for abortion, septicaemia and meningitis in animals and man. listeria ivanovii is a primary cause of abortion in animals. owing to a number of epidemics and single cases caused by food contaminated with l. m ... | 1991 | 1782429 |
| [preliminary serological survey on mycoplasma and chlamydia infection among gonorrhoea cases]. | the antibodies to mycoplasma hominis (mh) and chlamydia trachomatis (ct) in 123 sera from the patients with gonorrhoea and 108 sera from the healthy persons control were detected with micro-indirect hemagglutination test. the results showed that the antibody to mh positive rate was 23.58%, gmt 1: 7.60, and to ct 72.35%, gmt 1: 8.71 in the patients. the differences of the antibody levels between the patients and the controls were significant. it was suggested that the urogenital infections caused ... | 1991 | 1782655 |
| relation between renal and hepatic excretion of drugs. xiv. elimination of ioglycamic acid after nephrectomy, bile duct ligation, and after treatment with hormones or xenobiotics in young and adult rats. | ioglycamic acid (iga) is effectively eliminated in young and adult rats via urine and bile. after administration of low doses hepatic excretion dominates whereas following high supply renal elimination surpasses biliary excretion. hepatic transport of iga is active, indicated by the occurrence of a transport maximum in vivo and by a distinct accumulation of this drug within liver slices in vitro. renal removal of iga is preferentially caused by glomerular filtration. a tubular reabsorption obvio ... | 1991 | 1783049 |
| [detection of chlamydia trachomatis from urethral swab in male urethritis by polymerase chain reaction]. | a polymerase chain reaction (pcr) with chlamydia trachomatis-specific primers was applied for detection of c. trachomatis from urethral swab in male urethritis. the results were compared with those of culture method for detection of c. trachomatis. of 18 clinical specimens tested in this study, inclusion bodies of c. trachomatis were detected in 11 specimens by the culture method. for pcr, sample dna was prepared from transport medium in which urethral smear was suspended and two oligonucleotide ... | 1991 | 1783806 |
| [isolation and identification of chlamydia spp. from effusion of otitis media]. | three chlamydial strains isolated from patients of otitis media with effusion were studied by comparing reactivity to monoclonal antibody (mab) and polyclonal antibody (pab) produced against one clinical isolate (named mk), which was first isolated by dr mukai (mukai microbiological research laboratory, yamato-shi, kanagawa prefecture). commercially supplied antibody (microtrak (syva), culture-set (ortho diagnostic system)) was also used. to isolate the chlamydia spp, the yolk sacs of eggs were ... | 1991 | 1783812 |
| effects of feeding flax and two types of sunflower seeds on fatty acid compositions of yolk lipid classes. | single comb white leghorn laying hens were fed diets high in oleic, linolenic, or linoleic acid prepared by incorporation of high oleic acid sunflower seed (hoas), full-fat flax seed (flax), or regular high linoleic acid sunflower seed (hlas), respectively, to investigate the effects of dietary fats on the fatty acid compositions of major lipid classes of chicken eggs. egg production, haugh units, specific gravity, and yolk total lipid content were measured. after 3 wk of feeding, the fatty acid ... | 1991 | 1784568 |
| possible noradrenergic involvement in training stimulus intensity. | day-old chicks trained on a single trial passive discrimination avoidance task using a concentrated chemical aversant, methyl anthranilate (mea), have been shown to exhibit three stages of memory processing: short, intermediate and long term. a similar learning task with the aversant diluted to 20% in ethanol leads to short- and intermediate-term memory only, but not to long-term memory. the emergence of long-term memory has been shown to be associated with the production of a nonenergy-dependen ... | 1991 | 1784600 |
| glutathione deficiency produced by inhibition of its synthesis, and its reversal; applications in research and therapy. | glutathione, which is synthesized within cells, is a component of a pathway that uses nadph to provide cells with their reducing milieu. this is essential for (a) maintenance of the thiols of proteins (and other compounds) and of antioxidants (e.g. ascorbate, alpha-tocopherol), (b) reduction of ribonucleotides to form the deoxyribonucleotide precursors of dna, and (c) protection against oxidative damage, free radical damage, and other types of toxicity. glutathione interacts with a wide variety ... | 1991 | 1784629 |
| [listeria meningitis and hiv infection]. | although listeria monocytogenes is an opportunistic pathogen, infection by this bacteria is infrequent in aids patients. we were describe a case of l. monocytogenes meningitis in a patient infected by the human immunodeficiency virus (hiv). literature is reviewed, and the pathogenesis of infection by this bacteria and its relation to aids is discussed. | 1991 | 1784745 |
| [current status of the antipneumococcal vaccine: a pending issue]. | in spite of antibiotic treatment, mortality due to pneumonia caused by streptococcus pneumoniae is still considerable and it is related to the presence of bacteremia. prophylaxis with 23-polyvalent anti-pneumococcus vaccine would prevent severe processes in high risk populations. we present in this work the current status of our knowledge about this vaccine, regarding its efficacy in preventing bacteremia, economic cost, and secondary effects, we point out the indications of this vaccine accordi ... | 1991 | 1784783 |
| [hypereosinophilic syndrome with gastrointestinal involvement and pulmonary nocardiosis]. | | 1991 | 1784789 |
| evidence of chlamydia infection in a belfast antenatal population. | chlamydia trachomatis is an important cause of postpartum endometritis and neonatal conjunctivitis. however, the prevalence of chlamydial genital infection varies considerably from one population group to another. a study was thus conducted to determine the incidence of c trachomatis infection of the cervix in an unselected group of women attending a belfast antenatal clinic. one hundred and six patients were screened for evidence of current cervical infection with c trachomatis or serological e ... | 1991 | 1785151 |
| screening for chlamydia trachomatis infection in women and aspects of the laboratory diagnostics. | nine hundred and thirty-three women who attended consecutively a gynecological clinic for various symptoms, for abortion, or for contraceptives, were screened for genital chlamydia trachomatis infection. a total of 95 (10.2%) women were found infected. of women below 25 years of age, 13.4% were infected, as compared with 4.9% over 25. women with symptoms of genital infection were not over-represented in the infected population. enzyme immunoassays (eia) verified with a direct fluorescence antige ... | 1991 | 1785275 |
| preferential binding of chlamydia trachomatis to subsets of human lymphocytes and induction of interleukin-6 and interferon-gamma. | the interactions between chlamydia trachomatis and human blood mononuclear leukocytes were studied using flow cytometry, immunofluorescence, electron microscopy and cytokine assays. under serum-free conditions, elementary bodies (eb) of c. trachomatis were found to bind to human t lymphocytes as well as to b cells and monocytes/macrophages (m phi). for all cell types the binding was saturable, rapid, temperature-independent and independent of the chlamydia-specific serological status of the dono ... | 1991 | 1787004 |
| the limulus amoebocyte lysate assay for prediction of chlamydial infection in males with non-gonococcal urethritis: an evaluation. | chlamydia trachomatis produces small amounts of an endotoxin-like material. the limulus amoebocyte lysate assay was used to evaluate chlamydial cell cultures and also the exudates from adult male patients with non-gonococcal urethritis, as a possible method to subdivide this condition into chlamydial and nonchlamydial urethritis. in vitro endotoxin assays were conducted in mccoy cell media using the limulus assay, and endotoxin levels were consistently 10-fold less at 24 h than at 0, 48, 72, and ... | 1991 | 1787774 |