| evaluation of a chemiluminometric immunoassay for detection of chlamydia trachomatis in the urine of male and female patients. | a chemiluminometric immunoassay (magic lite chlamydia) for detection of chlamydia trachomatis antigens in first-void urine samples was compared with cell culture using urogenital swabs from 221 men and 242 women. the rate of isolation of chlamydia trachomatis was 23.5% in men, nearly 80% of whom had symptoms of urethritis, and 8.3% in women, in whom both cervix and urethra samples were tested. in urine sediments from men and women respectively the chemiluminometric assay showed a sensitivity of ... | 1992 | 1425728 |
| chlamydial genital infections and laparoscopic findings in infertile women. | several studies have shown that previous chlamydial genital infection, reflected by serological markers, is strongly associated with tubal damage leading to tubal infertility. in 105 women undergoing laparoscopy, multiple samples were collected from the lower (urethra and cervix) and upper (endometrium, peritoneal fluid, tubal lumen) genital tract, in order to isolate chlamydia trachomatis in cell culture. chlamydia trachomatis was isolated from at least one site in 13 (30.9%) of 42 infertile wo ... | 1992 | 1426163 |
| the association between sexually transmitted diseases and inflammatory cervical cytology. | to assess the significance of inflammatory changes as a marker of sexually transmitted diseases and their use as a diagnostic aid. | 1992 | 1427801 |
| screening for asymptomatic chlamydia trachomatis infection in adolescent men by examination of voided urine. | | 1992 | 1427813 |
| structure and function of the cd16:zeta:gamma complex expressed on human natural-killer cells. | natural-killer (nk) cells are large granular lymphocytes involved in host defense against tumor cells and virally-infected cells. in addition to natural cytotoxicity, nk cells can effect antibody-dependent cytotoxicity mediated by cd16 (fc gamma riiia alpha). it has recently been shown that cd16 is associated with disulfide-linked dimers composed of 2 homologous sub-units, zeta and gamma. these transducing molecules are also associated with other multimeric cell-surface receptors such as the t-c ... | 1992 | 1428397 |
| teenage girls attending a dublin sexually transmitted disease clinic: a socio-sexual and diagnostic profile. | over a 12 month period, 32 teenage girls attended the sexually transmitted disease clinic in the mater misericordiae hospital, dublin for the first time, accounting for 17.8% of all first visits. their mean age was 18.2 years (range 15-19 years). twenty-four (75%) were from social class v. five (15.6%) were abusing drugs. the mean age of first coitus was 16.1 years (range 13-19 years). the mean number of sexual partners was 1.8 (range 1-5). four (12.5%) had been sexually abused in the past. four ... | 1992 | 1428769 |
| acquisition and synthesis of folates by obligate intracellular bacteria of the genus chlamydia. | we undertook studies focused on folate acquisition by chlamydia trachomatis l2, chlamydia psittaci 6bc, and c. psittaci francis. results from in situ studies, using wild-type host cells, confirmed that c. trachomatis l2 and c. psittaci 6bc are sensitive to sulfonamides whereas c. psittaci francis is resistant. in addition c. trachomatis l2 and c. psittaci francis were inhibited by methotrexate in situ whereas c. psittaci 6bc was not. in contrast to c. trachomatis, neither c. psittaci strain was ... | 1992 | 1430206 |
| association of genital infection with specific chlamydia trachomatis serovars and race. | black race is an important risk marker for chlamydia trachomatis genital infection. to define whether c. trachomatis serovars differ by ethnic distribution, a panel of monoclonal antibodies was used to serotype 934 urethral and 581 cervical isolates from patients attending a sexually transmitted diseases clinic over 2 years. the overall serovar distribution in cervical and urethral infections was comparable, with b class serovars predominating. significantly higher inclusion counts were observed ... | 1992 | 1431263 |
| [detection of chlamydia trachomatis in first-voided urine sediments from male urethritis by polymerase chain reaction]. | chlamydia trachomatis was detected from first-voided urine sediments of 97 male patients with urethritis by polymerase chain reaction (pcr). since urine and urinary sediments only treated with proteinase k inhibited dna amplification by pcr, dna was further purified by phenol extraction and concentrated. two oligonucleotides based on sequences within the major outer membrane gene from c. trachomatis serovar l2 were used as primers. a dna fragment of 242 bp specific for c. trachomatis was amplifi ... | 1992 | 1431362 |
| chlamydial epididymitis diagnosed by genetic detection of chlamydia trachomatis from epididymal aspirate by polymerase chain reaction. | | 1992 | 1431375 |
| [detection of neisseria gonorrhoeae in first-voided urine sediments from male urethritis patients by polymerase chain reaction]. | neisseria gonorrhoeae was detected from first-voided urine sediments of male patients with urethritis by polymerase chain reaction (pcr). urine and urinary sediment were treated with proteinase k, and dna was further purified by phenol extraction. two oligonucleotides based on sequences within a ribosomal rna gene from n. gonorrhoeae were used as primers for the pcr. a dna fragment of 206 bp specific for n. gonorrhoeae was amplified by pcr and detected by agarose gel electrophoresis. in 19 speci ... | 1992 | 1431378 |
| an interesting case presentation: a possible new route for perinatal acquisition of chlamydia. | chlamydia trachomatis is currently the most common sexually transmitted disease in the united states. the prevalence in pregnant women ranges between 2% and 47%. it is well known that c trachomatis can be transmitted from the genital tract of an infected mother to her newborn infant, causing conjunctivitis or pneumonia or both, along with their longstanding complications. a review of the literature failed to show, however, conclusive evidence of transmission of infection to the newborn infant wh ... | 1992 | 1432291 |
| group b streptococcal sepsis. | | 1992 | 1433683 |
| the tetracyclines. | the tetracyclines are inexpensive drugs that are of primary value in treating nonpregnant women who have acute urethral syndrome or endocervicitis due to chlamydia trachomatis. they are contraindicated in pregnancy because they are injurious to fetal teeth and bone. | 1992 | 1436932 |
| [chlamydia trachomatis infection of the urogenital system in our clinical experience]. | the increased number of the genitourinary system infection caused by chlamydia trachomatis (ch. tr.), increased number of patients with dysuria or sterile leukocyturia gave stimulus to studies of 615 patients from department of nephrology and district outpatient nephrological care unit with regard to infections with that microbes. material for investigations derived from urethra. diagnostic examinations were performed using the mc coy cell culture and the immunofluorescence method. the infection ... | 1992 | 1438915 |
| late airway changes caused by chronic rejection in rat lung allografts. | airway disease after lung or heart-lung transplantation is one of late major complications, affecting the prognosis of the transplants. little is known about the causes of airway changes. we performed rat lung transplantation and investigated the late airway changes of the long-term surviving lung grafts: allografts, bn to lewis; isografts, bn to bn rat. all recipients were treated with csa. we found airway changes, i.e., mucosal ulceration, granulation, submucosal fibrosis, which was located in ... | 1992 | 1440846 |
| antibodies to chlamydia trachomatis in sera of women with recurrent spontaneous abortions. | the relationship between high-titer immunoglobulin g antibodies to chlamydia trachomatis and recurrent spontaneous abortions was evaluated. | 1992 | 1442916 |
| changes in enzymatic and membrane-adsorbing activities of sphingomyelinase from bacillus cereus by modification with a polyethylene glycol derivative. | sphingomyelinase (smplc) from bacillus cereus was modified with a polyethylene glycol (peg) derivative, methoxypolyethylene glycol-succinimidyl succinate (ss-peg). the molecular weight of the ss-peg-modified smplc was calculated to be approx. 150 kda by gel-filtration whereas that of the native enzyme, was 25 kda. by this modification, the enzyme increased its thermostability and retained its hydrolytic activity toward 2-(n-hexadecanoylamino)-4-nitrophenylphosphocholine (hnp) and sphingomyelin ( ... | 1992 | 1446369 |
| phorbol ester activation of the protein kinase c pathway inhibits gonadotropin-releasing hormone gene expression. | the effects of the phorbol ester 12-o-tetradecanoyl phorbol 13-acetate (tpa), an activator of protein kinase c (pkc), and the pkc inhibitor staurosporine on gnrh secretion and mrna levels were studied in gt1-7 hypothalamic neuronal cells. dose-response and time-course studies revealed that tpa (10(-8) m) acutely increased gnrh secretion 3-fold at 3-6 h, which then declined to baseline at 24 h, while it progressively decreased gnrh mrna levels by 50% and 70% at 6 and 24 h, respectively. to ensure ... | 1992 | 1446598 |
| the diagnosis and treatment of lyme disease. | lyme disease has probably been reported for at least 80 years, with the cause unknown until the discovery of the ixodes ricinus complex tick-borne spirochete b. burgdorferi. the incidence of diagnosed cases appears to be increasing in kansas and elsewhere. a negative laboratory test does not exclude the possibility of the disease. at present, no accurate laboratory screen for the illness is available. the diagnosis must be considered when acute or chronic clinical manifestations reviewed in this ... | 1992 | 1447864 |
| interaction between ciprofloxacin and thiopental in the central nervous system of the male rat. | the effect of intravenous ciprofloxacin (cpx) pretreatment on the kinetics and brain sensitivity for thiopental was studied in male rats using a previously developed electroencephalographic (eeg) threshold method. thiopental was administered intravenously with constant infusion rate. immediately after the appearance of the first burst suppression of 1 sec. or more (the "silent-second") in the eeg the infusion was stopped and the rats were killed by decapitation. the dose of thiopental needed to ... | 1992 | 1448448 |
| enzyme electrophoretic polymorphism differentiates invasive from non-invasive chlamydia psittaci ruminant isolates. | a group of 24 chlamydia psittaci strains isolated from ruminants, belonging to serotype 1 and previously classified as invasive in a mouse model of virulence, was compared to a group of 10 non-invasive strains belonging to serotype 2 by using determination of glucose-6-phosphate and l-malate dehydrogenase zymotypes resulting of the infection of cells by these strains. the serotype 1 or invasive isolates represent a homogeneous group by sharing a unique zymotype which was not observed in the non- ... | 1992 | 1448629 |
| association between chlamydial infections and pelvic lesions. | chlamydia trachomatis usually causes asymptomatic cervicitis, but it sometimes ascends into the uterine cavity, fallopian tubes, or peritoneal cavity, causing pelvic inflammatory disease and infertility. in this study, we examined endocervical chlamydial antigens and serum chlamydial antibodies in infertile women and laparoscopically evaluated pelvic lesions according to our pelvic scoring system. in patients testing positive for a chlamydial infection, the total pelvic score was significantly h ... | 1992 | 1449424 |
| frequency of antigen-specific b cells during experimental ocular chlamydia trachomatis infection. | chlamydia-specific antibody-secreting cells have been identified in conjunctiva and draining cervical lymph nodes by an elispot assay in a cynomolgus monkey model of trachoma. these local sites contained numbers of chlamydia-specific b cells that were higher than those in distant inguinal lymph nodes and peripheral blood. the numbers of chlamydia-specific immunoglobulin g-secreting b cells observed were 5 to 57 per 10(6) cells in conjunctiva and 24 to 996 per 10(6) cells in cervical lymph nodes ... | 1992 | 1452363 |
| inhibition of chlamydia trachomatis growth in mouse fibroblasts by liposome-encapsulated tetracycline. | the in-vitro susceptibility of chlamydia trachomatis to liposome-encapsulated tetracycline was determined and compared with free tetracycline. anionic, cationic and neutral small unilamellar liposomes were used in this study. chlamydia-infected mouse fibroblast monolayers were continuously exposed to varying concentrations of antibiotic, incubated for 48 h and giemsa stained. the minimum inhibitory concentration (mic) for anionic, cationic and neutral liposomes containing tetracycline were 0.38, ... | 1992 | 1452495 |
| multicenter evaluation of the antigenz chlamydia enzyme immunoassay for diagnosis of chlamydia trachomatis genital infection. | to evaluate the antigenz chlamydia enzyme immunoassay (eia) (baxter/bartels, issaquah, wash.), we studied 320 men and 1,209 women attending clinics for sexually transmitted diseases in baltimore, san francisco, and seattle. at examination, two separate swabs were obtained from each patient, one for chlamydial culture and one for eia. cervical samples were collected from women, and urethral samples were collected from men. the prevalence of chlamydial infection by culture was 9% in baltimore (n = ... | 1992 | 1452643 |
| evaluation of three immunoassays for detection of chlamydia trachomatis in urine specimens from asymptomatic males. | the performances of three commercially available immunoassays (chlamydiazyme/antibody blocking assay [abbott diagnostics, abbott park, ill.], ideia [analytab products, plainview, n.y.], and microtrak eia [syva co. palo alto, calif.]) were evaluated for the detection of chlamydia trachomatis in urine specimens from asymptomatic males. assay results were compared with direct specimen immunofluorescence (dfa) analysis of urine sediment (syva microtrak; syva co.), which was chosen as the study confi ... | 1992 | 1452648 |
| detection of chlamydia trachomatis in endocervical specimens by polymerase chain reaction. | a rapid and sensitive polymerase chain reaction (pcr)-based assay for detection of chlamydia trachomatis in cervical specimens is described. this assay consists of (i) sample preparation which avoids the use of heat, centrifugation, or organic extractions; (ii) rapid, two-temperature pcr amplification of c. trachomatis cryptic plasmid sequences; and (iii) capture and colorimetric detection of amplified dna in microwell plates. pcr was compared with culture by using 503 cervical specimens. after ... | 1992 | 1452654 |
| establishment of a particle-counting method for purified elementary bodies of chlamydiae and evaluation of sensitivities of the ideia chlamydia kit and dna probe by using the purified elementary bodies. | to evaluate the sensitivity of commercially available test kits for detection of chlamydiae, we established a method of purifying chlamydia trachomatis and chlamydia pneumoniae elementary bodies (ebs). we then subjected the purified ebs, together with the purified ebs of chlamydia psittaci, to the ideia chlamydia (ideia) and dna probe test kits to determine the eb numbers at the detection limits. the sensitivities of the test kits were thus compared. the results can be summarized as follows. (i) ... | 1992 | 1452662 |
| comparison of two culture approaches, blind passage and dual observation, for detecting chlamydia trachomatis in various prevalence populations. | chlamydia trachomatis diagnosis in our laboratory consisted of dual inoculation of shell vials and detection of inclusions by using fluorescein-conjugated monoclonal antiserum; the second culture vial was conventionally used for blind passage when the first vial was negative. we compared the increase in positivity using blind passage with that of a strategy utilizing observation of two stained monolayers (dual observation) without blind passage, in an effort to reduce the reporting time and labo ... | 1992 | 1452664 |
| comparison of cytobrushes with swabs for recovery of endocervical cells and for chlamydiazyme detection of chlamydia trachomatis. | endocervical swab and cytobrush specimens from 1,301 symptomatic women were microscopically analyzed for adequacy and tested using chlamydiazyme (cz) (abbott laboratories). when the swab specimen was collected first, blocking antibody-confirmed cz-positive results were obtained from 48 (8.0%) of 599 patients, 42 (87.5%) from swabs and 46 (95.8%) from cytobrushes (not significant). when the swab specimen was collected second, confirmed cz-positive results were obtained from 46 (6.6%) of 702 patie ... | 1992 | 1452671 |
| three-dimensional structure of the high-potential iron-sulfur protein isolated from the purple phototrophic bacterium rhodocyclus tenuis determined and refined at 1.5 a resolution. | the molecular structure of the high-potential iron-sulfur protein (hipip) isolated from the phototrophic bacterium, rhodocyclus tenuis, has been solved and refined to a nominal resolution of 1.5 a with a crystallographic r-factor of 17.3% for all measured x-ray data from 30 a to 1.5 a. it is the smallest of the hipip structures studied thus far with 62 amino acid residues. crystals used in the investigation belonged to the space group p2(1) with unit cell dimensions of a = 36.7 a, b = 52.6 a, c ... | 1992 | 1453470 |
| epidemiology of adult sexually transmitted disease agents in children being evaluated for sexual abuse. | this prospective study describes the epidemiology of adult sexually transmitted disease agents in 1538 children ages 1 to 12 years being evaluated for possible sexual abuse. infections with these agents were related to the presence or absence of a history of sexual contact. neisseria gonorrhoeae (gc) was found in 2.8% (41 of 1469); human papillomavirus presenting as condyloma acuminata, 1.8%; chlamydia trachomatis, 1.2% (17 of 1473); treponema pallidum (syphilis), 0.1% (1 of 1263); and herpes si ... | 1992 | 1454437 |
| chlamydial infection in canada. | | 1992 | 1458406 |
| anti-enterobacteria antibodies in psoriatic arthritis. | the occurrence of certain antibacterial antibodies was studied in the sera of 22 healthy donors (hd) and 66 patients with different diseases. the cases investigated included 22 rheumatoid arthritis (ra), 22 non-arthritic-psoriasis (nap), and 22 psoriatic arthritis (pa) patients. a complement fixation test was used with yersinia enterocolitica 0:3 type (yec), yersinia pseudotuberculosis (ypt), campylobacter jejuni (cj), and campylobacter fetus (cf) antigens; the detection of anti-chlamydia tracho ... | 1992 | 1458698 |
| analysis of soluble human and mouse interferon-gamma receptors expressed in eukaryotic cells. | the extracellular domains of the human and mouse interferon-gamma receptors were produced in insect spodoptera frugiperda cells infected with recombinant baculoviruses and in mammalian chinese-hamster-ovary cells. the receptors expressed in both systems are secreted into the culture medium. their signal peptides are cleaved off and the proteins show heterogeneity in glycosylation which, however, does not affect the capacity to bind interferon gamma or specific antibodies. the soluble mouse recep ... | 1992 | 1459135 |
| chlamydia antibody by enzyme-linked immunosorbent assay and associated severity of tubal factor infertility. | we have evaluated the clinical utility of the serum chlamydial antibody value by elisa in predicting the severity of tubal factor infertility quantified by the use of a previously reported pelvic scoring system. significant differences in salpingitis scores, tubal occlusion scores, adhesion scores, and pelvic stages were found with increasing chlamydia antibody grades. the level of elisa value strongly correlated with salpingitis, tubal occlusion, and total pelvic scores and pelvic stages. the s ... | 1992 | 1459280 |
| analysis of genomic dna from chlamydia trachomatis for dam and dcm methylation. | chlamydia trachomatis is a gram-negative eubacterium with a dimorphic developmental cycle and obligate intracellular growth in the eucaryotic host. the dam transmethylase of escherichia coli methylates at the n6 position of adenine in the sequence 5'-gatc-3' and the dcm transmethylase adds methyl groups to the c5 position of the internal cytosines in the sequences 5'-ccwgg-3'. in contrast to e. coli, c. trachomatis dna appears to have unmethylated dam sites and only low level dcm methylation. | 1992 | 1459404 |
| effects of interleukin-1 beta and tumor necrosis factor-alpha on osteoblastic expression of osteocalcin and mineralized extracellular matrix in vitro. | osteoblasts play a pivotal role during the bioresponse of bone to agents that stimulate bone resorption and/or inhibit bone formation including hormones, polypeptide growth factors, and cytokines. we examined the cytokines interleukin-1-beta (il-1 beta) and tumor necrosis factor-alpha (tnf-alpha) for their effects on osteoblastic proliferation and development and expression of alkaline phosphate and the osteoblast-specific protein osteocalcin in a mineralizing environment. primary rat osteoblast ... | 1992 | 1459694 |
| [declining incidence of chlamydia--increasing diagnostic demands]. | | 1992 | 1460973 |
| [chlamydia trachomatis in young conscripts--survey, urine analysis and urethral culture]. | | 1992 | 1460983 |
| [decisional analysis in the diagnosis of chlamydia trachomatis]. | the aim of this work is the use of decisional analysis for the clinical diagnosis of chlamydia trachomatis, to verify the utility of making a routine direct immunofluorescence test with a strategy of no routine direct immunofluorescence test with a strategy of no routine test in women during gynecologic escannations. patients eligible for this analysis were sexually active woman, who are not pregnant, woman in reproductive age who do not have evidence of a clinical syndrome associated with or kn ... | 1992 | 1461550 |
| [genital chlamydia trachomatis infection in abortion seekers. strategy of examination and treatment in order to reduce the sequelae of the infection]. | among women applying for termination of pregnancy, 5-17% are infected with chlamydia trachomatis. the prevalence is higher among women under 20 years of age (12-30%), in nulliparae and when neisseria gonorrhoeae are found simultaneously. if women applying for termination of pregnancy with chlamydia infection are not treated, 10-60% will develop pelvic infection after abortion. salpingitis caused by chlamydia runs a milder clinical course than salpingitis caused by gonococci or other etiology but ... | 1992 | 1462399 |
| [presence of genital chlamydia trachomatis in abortion seekers--correlates with young age and nulliparity but not with previous genital infection]. | out of 432 women applying for termination of pregnancy, 7.9% (34/428) had cervical chlamydia trachomatis and 0.7% (3/431) genital neisseria gonorrhoeae. the prevalence of chlamydia was 19.2% among the women applying for termination who were under 20 years and 12.8% among those aged 21-25 years. the finding of chlamydia among nulliparae was 14.5%. only 2.8% of the women with chlamydia had previously had pelvic infections. women with chlamydia did not have significantly greater frequency of previo ... | 1992 | 1462400 |
| [preventive antibiotics in induced first-trimester abortion]. | the frequency of infection following induced first-trimester abortion is 3-5%. duration of hospitalization is often five days, and the total costs per abortion were 5,400 dkr (approximately pounds 500) in denmark in 1979. sequelae of postabortal infection are similar to and occur with the same frequency as sequelae to "spontaneous" pelvic inflammatory disease. thus, secondary infertility was found in 10% of women with postabortal infection, spontaneous abortion in 22%, dyspareunia in 20%, and ch ... | 1992 | 1462401 |
| characteristics of polymorphism at a vntr locus 3' to the apolipoprotein b gene in five human populations. | we have analyzed the allele frequency distribution at the hypervariable locus 3' to the apolipoprotein b gene (apob 3' vntr) in five well-defined human populations (kacharis of northeast india, new guinea highlanders of papua new guinea, dogrib indians of canada, pehuenche indians of chile, and a relatively homogeneous caucasian population of northern german extraction) by using the pcr technique. a total of 12 segregating alleles were detected in the pooled sample of 319 individuals. a fairly c ... | 1992 | 1463014 |
| [the pathogenic role of chlamydia trachomatis in otitis media with effusion]. | there are conflicting views concerning middle ear infections due to chlamydia trachomatis. to ascertain the etiological role of this agent in otitis media with effusion, middle ear effusions were cultured for c. trachomatis and other bacterial flora. a total of 102 patients with otitis media with effusion (ome) were recruited for this study. the study population included 66 patients with acute ome (aome) and 36 patients with chronic ome (come). as chlamydia pneumoniae, the third species of chlam ... | 1992 | 1464792 |
| aetiological role of bacteria associated with reactive arthritis in pauciarticular juvenile chronic arthritis. | the cause of juvenile chronic arthritis (jca) is unknown. pauciarticular jca is the most common subtype and can be subdivided into early (type i) and late onset (type ii) forms, the latter clinically resembling reactive arthritis. | 1992 | 1466598 |
| association of cervicovaginal infections with increased vaginal fluid phospholipase a2 activity. | the purpose of this study was to determine if phospholipase a2 was detectable within vaginal fluid and to correlate its presence with the presence of common lower genital tract infection or microbial conditions. | 1992 | 1471671 |
| modulation of the low-density-lipoprotein-receptor-related protein and its relevance to chylomicron-remnant metabolism. | hepatic levels of the low-density-lipoprotein (ldl)-receptor-related protein (lrp) and the ldl receptor were measured in rats subjected to treatments known to affect the expression of the ldl receptor. propylthiouracil decreased both hepatic lrp and ldl receptor expression by 30-40%. thyroxine treatment increased ldl receptor levels by 3-fold without altering lrp levels. in contrast, 17 alpha-ethinyloestradiol decreased lrp by 50%, whereas the ldl receptor was increased 5-fold. plasma chylomicro ... | 1992 | 1471994 |
| lack of circumcision increases the risk of urinary tract infection in young men. | to determine whether lack of circumcision increases the risk of urinary tract infection (uti) in adult men. | 1992 | 1472171 |
| partner notification and patient education for cases of chlamydia trachomatis infection in a rural nova scotia health unit. | from september 1990 to march 1991, 47 of 52 patients with genital c. trachomatis infections in a rural health unit in nova scotia were interviewed about preventive education by physicians partner notification, past history, and treatment. seventy-six percent of those with partners of the previous month were instructed by physicians to notify these partners. twenty-five of 37 partners were notified. of those not notified, 83% could have been reached. reasons for cases not notifying partners inclu ... | 1992 | 1473063 |
| [diagnosis of gonococcal urethritis and chlamydial urethritis by polymerase chain reaction]. | a polymerase chain reaction (pcr) method was compared to standard methods (cultures for neisseria gonorrhoeae and chlamydia trachomatis and an enzyme-immunoassay for c. trachomatis) in diagnosis of gonococcal and chlamydial urethritis in 40 male patients with urethritis. gonococcal urethritis was diagnosed by detection of a 206 bp dna fragment amplified by pcr with n. gonorrhoeae-specific primers. chlamydial urethritis was diagnosed by detection of a 242 bp dna fragment amplified by pcr with c. ... | 1992 | 1474717 |
| chlamydia trachomatis confirmatory testing of pcr-positive genitourinary specimens using a second set of plasmid primers. | a plasmid-based pcr for the detection of chlamydia trachomatis was evaluated with a confirmatory pcr employing a second set of plasmid primers. a total of 258 genitourinary specimens including 134 female endocervical and urethral specimens and 124 male urethral specimens were tested by culture, blocked eia and pcr. fifty-four specimens were positive by culture, 50 were positive by eia and 71 were positive by pcr. fourteen specimens that were pcr-positive but culture- and eia-negative were confir ... | 1992 | 1474977 |
| a longitudinal study of pelvic inflammatory disease. | to study the microbiology and long term prognosis of pelvic inflammatory disease (pid). | 1992 | 1477024 |
| evaluation of new anti-infective drugs for the treatment of chancroid. infectious diseases society of america and the food and drug administration. | chancroid is a mucocutaneous infection caused by haemophilus ducreyi that produces ulcerative lesions and enhances the efficiency of transmission of human immunodeficiency virus (hiv). confirmation of infection by culture of h. ducreyi is essential in therapeutic trials. minimal inhibitory concentrations of antibiotics for the isolate should be determined by agar dilution. patients should be evaluated by appropriate laboratory tests for syphilis, infection with herpes simplex virus, gonorrhea, a ... | 1992 | 1477217 |
| evaluation of new anti-infective drugs for the treatment of uncomplicated gonorrhea in adults and adolescents. infectious diseases society of america and the food and drug administration. | gonorrhea is among the most common sexually transmitted diseases. treatment for uncomplicated gonorrhea should be efficacious in > or = 95% of cases. because patients with gonococcal infections often have other sexually transmitted diseases concurrently, individuals enrolled in clinical trials of therapy for gonorrhea should also be evaluated for infection with chlamydia trachomatis and for syphilis. testing for other pathogens should be considered in light of the clinical presentation. the pres ... | 1992 | 1477219 |
| evaluation of new anti-infective drugs for the treatment of sexually transmitted chlamydial infections and related clinical syndromes. infectious diseases society of america and the food and drug administration. | this guideline addresses clinical trials of new antimicrobial agents in the treatment of uncomplicated genital infections caused by chlamydia trachomatis and of syndromes resembling chlamydial infections. the most common clinical manifestations of chlamydial infection are urethritis in men and mucopurulent cervicitis in women. however, many chlamydial infections are not associated with inflammatory symptoms or signs. culture is the diagnostic standard for defining the presence of c. trachomatis, ... | 1992 | 1477220 |
| evaluation of new anti-infective drugs for the treatment of acute pelvic inflammatory disease. infectious diseases society of america and the food and drug administration. | pelvic inflammatory disease (pid) is a syndrome unrelated to pregnancy or surgery and characterized by lower abdominal pain and tenderness, cervical motion tenderness, and adnexal tenderness. fever, leukocytosis, and the results of laboratory tests are used to support the diagnosis. participants in clinical trials should be stratified into two groups: those with and those without tubo-ovarian abscess--i.e., those with complicated and those with uncomplicated pid. diagnostic studies and treatment ... | 1992 | 1477252 |
| immunomagnetic recovery of chlamydia trachomatis from urine with subsequent colorimetric dna detection. | there is need for integrated systems that provide rapid and sensitive diagnosis of chlamydia trachomatis. here we describe for the first time that specific antibodies to a c. trachomatis surface protein can be used for magnetic recovery of bacteria directly from crude urine samples. the combination of immunomagnetic separation (ims), where the bacterial cells are both purified and concentrated, with a colorimetric pcr assay makes the method sensitive and suitable for routine clinical screening i ... | 1992 | 1477673 |
| specificity of the microimmunofluorescence assay for the serodiagnosis of chlamydia pneumoniae infections. | chlamydia pneumoniae infections are mostly confirmed using an indirect microimmunofluorescence test for which potential cross-reactions between antigens from different chlamydial species are not well documented. using this assay, 928 sera (507 subjects) submitted for chlamydia pneumoniae serology were tested for specific igm and igg to this bacteria using the tw-183 antigen. igm and igg reactivities to chlamydia trachomatis serotypes c, d, e, and l2 and chlamydia psittaci strain 6bc antigens wer ... | 1992 | 1477792 |
| evaluation of syva's enzyme immunoassay for the detection of chlamydia trachomatis in urogenital specimens. | a newly developed microwell enzyme immunosorbent assay (eia) system by syva company (palo alto, ca) can detect chlamydia trachomatis in < 3 hr. it uses a polyclonal antibody to chlamydial lipopolysaccharide and end points are determined with a spectrophotometer. three clinical trial sites (university of california medical center, san francisco, ca; university of washington, seattle, wa; and louisiana state university medical center, new orleans, la), compared this eia with tissue culture (tc) fo ... | 1992 | 1478048 |
| effect of minocycline on the sperm count and activity in infertile men with high pus cell count in their seminal fluid. | twenty-five infertile males with 5 or more pus cells per 1000 x field in their seminal fluid were randomly assigned to treatment with minocycline 200 mg daily for one (group a) or two (group b) weeks. at the end of the treatment period a statistically significant reduction of pus cells, and a statistically significant increase in the sperm count and the motile sperm were observed (wilcoxon matched-pairs signed-ranks test, p < 0.01). no statistically significant differences were observed between ... | 1992 | 1479417 |
| [updating on chlamydia trachomatis infections]. | | 1992 | 1480304 |
| [chlamydia infection and salpingitis]. | chlamydia trachomatis is the most prevalent etiologic agent in women with uncomplicated salpingitis. this presentation reviews the current aspects of etiology, diagnosis, therapy, prevention and complications of this disease. the other clinical manifestations of chlamydial infection are also discussed. | 1992 | 1481583 |
| gardnerella vaginalis infection and sexual contact in female children. | gardnerella vaginalis (gv) infection has been reported as being acquired via sexual contact in adults and as an indicator of sexual contact in female children (dejong, 1985). the purpose of this study was to determine if gv infection was more commonly found in 191 female children who gave a history of sexual contact and/or were infected with neisseria gonorrhoeae (gc) or chlamydia trachomatis (ct) (group 1), compared with 144 female children evaluated for possible sexual abuse and found to have ... | 1992 | 1486513 |
| chlamydial infections of the heart. | chlamydiae are common human pathogens, causing a broad spectrum of infectious diseases. chlamydial infections involving the heart have been described in numerous previous reports. these organisms are documented to cause endocarditis, myocarditis and pericarditis. furthermore, chlamydia pneumoniae, the recently discovered respiratory pathogen, has also been implicated in coronary artery disease. for the first time the literature on involvement of the heart in chlamydial infections is reviewed. in ... | 1992 | 1486883 |
| [remission of acute promyelocytic leukemia after all-trans-retinoic acid]. | this is the first report in israel of the successful treatment of acute promyelocytic leukemia (apl; m3) with an active metabolite of vitamin a. in a 42-year-old woman with apl all-trans-retinoic acid (atra; tretinoin), 45 mg/m2/day was given per os for 90 days. apl is associated with a distinct cytogenetic abnormality: translocation of a portion of the long arm chromosome 17 onto the long arm chromosome 15t (15; 17) with a breakpoint on chromosome 17 in the region of the retinoic acid receptor- ... | 1992 | 1487198 |
| the value of urine specimens in screening for male urethritis and its microbial aetiologies in tanzania. | to evaluate the first void urine (fvu) specimen in screening for urethritis and its microbial aetiologies in a male african population in which urinary schistosomiasis is also prevalent. | 1992 | 1487256 |
| ca2+ dependence of small ca(2+)-activated k+ channels in cultured n1e-115 mouse neuroblastoma cells. | single-channel properties of ca(2+)-activated k+ channels have been investigated in excised membrane patches of n1e-115 mouse neuroblastoma cells under asymmetric k+ concentrations at 0 mv. the sk channels are blocked by 3 nm external apamin, are unaffected by 20 mm external tetraethylammonium (tea) and have a single-channel conductance of 5.4 ps. the half-maximum open probability and opening frequency of sk channels are observed at 1 microm internal ca2+. concentration/effect curves of these pa ... | 1992 | 1488280 |
| [the subcellular localization of carboxypeptidase n in the substantia grisea of the cat brain]. | the activity of carboxypeptidase h in the cat grey matter is established to be connected with microsomes. a possibility to involve carboxypeptidase h to the processing of neuropeptide predecessors in the encephalon is discussed. | 1992 | 1488822 |
| re: "chlamydia trachomatis infection in women: a need for universal screening in high prevalence populations?". | | 1992 | 1488970 |
| oncogenes and tumor suppressor genes: new biochemical tests. | oncogenes and tumor suppressor genes are genes involved in cell proliferation and differentiation. they play a crucial role in the initiation and progression of cancer. some of these genes are found to be altered in human cancers, i.e., mutated, amplified, deleted, translocated, or abnormally regulated. recently, the protein products of the genes have been purified, and antibodies against them have been developed. studies of oncogenes and tumor suppressor genes at the dna, mrna, or protein level ... | 1992 | 1489520 |
| the role of neopterin as a monitor of cellular immune activation in transplantation, inflammatory, infectious, and malignant diseases. | the accumulated knowledge about the organization and function of the human immune system contributes to a better understanding of the pathogenesis of most diverse disorders and is opening new avenues for therapeutic regimens. to gain further insight into the complex interactions within the components of the immune system, it has become increasingly necessary to develop rapid and simple methods to monitor the status of the immune system in patients. the determination of neopterin concentrations i ... | 1992 | 1489521 |
| experimental podophyllotoxin (bajiaolian) poisoning: i. effects on the nervous system. | bajiaolian, one of the species in the mayapple family (podophyllum pelatum), has been widely used as a traditional chinese herbal medicine for the remedies of snake bites, general weakness, poisons, condyloma accuminata, lymphadenopathy, and certain tumors in china. in western medicine, podophyllum was first used medically as a laxative in the early 19th century. since 1940, the resin of podophyllum has also been used topically for various skin lesions, such as warts and condyloma. human poisoni ... | 1992 | 1489522 |
| [evaluation of the use of a rapid diagnostic technique (test pack) for chlamydia trachomatis in women]. | | 1992 | 1489782 |
| testing for cervical chlamydia trachomatis infection in an inner city practice. | the aims of the study were to find the prevalence of cervical chlamydia trachomatis infection in women attending for a speculum examination, to examine possible risk factors, and to see if we could develop a rational policy for testing for chlamydia in our deprived inner london practice. during 18 months 409 women aged 17-45 (mean 28) who attended for a cervical smear or vaginal examination were tested for chlamydia using the direct immunofluorescent test. they were assessed for possible risk fa ... | 1992 | 1490533 |
| prostatitis--diagnostic criteria, classification of patients and recommendations for therapeutic trials. | infections of the prostate may occur despite the numerous host defences of the male urogenital tract. it is important to distinguish patients with genuine inflammation of the gland from the larger number of men with symptoms but no signs of an inflammatory response (prostatodynia). to define prostatitis, the degree of the inflammatory reaction must first be determined. increased numbers of leucocytes in expressed prostatic secrections (eps) are essential for this diagnosis. careful lower urinary ... | 1992 | 1490749 |
| chlamydial associated syndrome of arthritis and eye involvement in young children. | the current classification of juvenile rheumatoid arthritis (jra) consists of several distinct subsets. we describe 6 children (2 boys, 4 girls, mean age 3.7 years, range 2.0-4.9 years) with arthritis and eye involvement associated with infection with chlamydia trachomatis. in some of the children, the clinical picture was similar to early onset pauciarticular jra: onset within the first 4 years of life, predominance of girls, pauciarticular arthritis, subacute uveitis, and presence of antinucle ... | 1992 | 1491404 |
| [urinary tract infection caused by chlamydia trachomatis in children]. | in 1988-1989, 131 children aged between 1 and 18 years were examined for urinary infections with c. trachomatis at the department of pediatric urology in wrocław. chlamydia trachomatis was diagnosed with the aid of mccoy's cellular cultures and immunoenzymatic test chlamydiazyme in 25% of the examined children. a relationship between chlamydia trachomatis and particular components of the clinical picture has been analysed. effectiveness of the treatment with erythromycin (davercin) and doxycycli ... | 1992 | 1492036 |
| sexually transmitted diseases in children. | sexually transmitted diseases (stds) in children may be transmitted by sexual abuse, by accidental contact, or perinatally. although only 2% to 10% of abused children become infected, childhood syphilis, gonorrhea, condylomata acuminata, and chlamydia trachomatis must always be considered. we reviewed data from our hospital regarding the frequency, prevalence, routes of transmission, and clinical features of these infections. ninety-five percent of acquired syphilis in children is transmitted by ... | 1992 | 1492047 |
| effects of broadening the gold standard on the performance of a chemiluminometric immunoassay to detect chlamydia trachomatis antigens in centrifuged first void urine and urethral swab samples from men. | traditionally, evaluations of nonculture assays for chlamydia trachomatis are based on a comparison with urethral culture in men and cervical culture in women as the standard for positivity of infection, but it is known that culture may be less than 100% sensitive. a chemiluminometric immunoassay, magic lite (ciba corning, medfield, ma) that detects c. trachomatis antigens was performed on centrifuged first void urine samples and urethral swabs collected from men attending a sexually transmitted ... | 1992 | 1492256 |
| compliance with antibiotic therapy for chlamydia trachomatis and neisseria gonorrhoeae. | control of bacterial sexually transmitted diseases (std) depends on adequate antibiotic therapy. during a 4-week period, a survey concerning compliance was administered to all patients attending an std clinic who were being treated with one of the standard antibiotic regimens for presumed gonococcal or chlamydial infections. of the 497 eligible patients, 406 (81.7%) were surveyed between 24 hours and 72 hours after the end of treatment. of those being treated with the standard 7-day regimen of t ... | 1992 | 1492264 |
| [genital infections with chlamydia trachomatis]. | | 1992 | 1492296 |
| chlamydia trachomatis antigen prevalence among pregnant women in west virginia. | during 1990, more than 2,100 women who received prenatal care at one of four clinics which serve 11 west virginia counties, were screened for chlamydial antigen. overall, 5.6 percent of the women screened had positive antigen tests and 90 percent of these individuals were under the age of 25. the prevalence of chlamydia was different at three geographic sites with the highest rate of positive antigen test being 9.4 percent at one site. these findings led to a careful analysis of the prevalence o ... | 1992 | 1492405 |
| [bacteriostatic and bactericidal activities of cyclines, macrolides and fluoroquinolones against chlamydia trachomatis]. | the in vitro activity of minocycline, doxycycline, erythromycin, roxithromycin, spiramycin, pefloxacin, and ofloxacin against ten c. trachomatis strains recovered from human genital tract specimens was evaluated. mac coy cell monolayers in 24-microwell plates were used. the c. trachomatis inoculum was 10(4) ifu/well. appropriate dilutions of antibiotic were added and inclusions were detected by immunofluorescence using monoclonal antibodies. mics were determined after 48 hours of exposure to eac ... | 1992 | 1495827 |
| [epidemiology of chlamydia trachomatis using analysis of gene encoding of the major outer membrane protein]. | one hundred and eight clinical strains and 24 reference strains of c. trachomatis were typed using differential restriction mapping of omp1, the gene which encodes the major outer membrane protein. the gene was obtained by polymerase chain reaction (pcr). this molecular typing method correlated well with serological typing. eighty-four per cent of clinical strains were typed using the enzyme alui alone. heterogeneity was looked for among the most common serovars (e, f, and d; 62%, 17%, and 9%, r ... | 1992 | 1495847 |
| prokaryotic dna replication. | | 1992 | 1497322 |
| immune specificity of murine t-cell lines to the major outer membrane protein of chlamydia trachomatis. | the antigenically variant chlamydia trachomatis major outer membrane protein (momp) is a target of antibody-mediated neutralization in vitro, and it is an important protein for designing a subunit vaccine. knowledge of momp t-cell determinants will be essential to elicit rapid and strong immune responses following an encounter with infectious organisms. c. trachomatis-specific t-cell lines were derived from momp-immunized balb/c mice and selected with intact organisms. we used these short-term t ... | 1992 | 1500180 |
| development and clinical evaluation of a polymerase chain reaction test for detection of chlamydia trachomatis. | a polymerase chain reaction (pcr) for the detection of chlamydia trachomatis was developed and evaluated. two primer-probe sets were designed; one detected a specific sequence of the plasmid, and the other detected the gene encoding the major outer membrane protein. both sets reacted species specifically and amplified sequences from all human serovars. a simple protocol was used for sample pretreatment. the pcr was optimized by addition of tetramethylammonium chloride and bovine serum albumin. t ... | 1992 | 1500521 |
| clearview chlamydia test for detection of chlamydiae in cervical specimens. | | 1992 | 1500539 |
| possible relationship between chlamydia trachomatis and acquired immunodeficiency syndrome. | this study suggests the possibility that the intracellular parasite chlamydia trachomatis (ct) may facilitate the transmission of the human immunodeficiency virus (hiv) during vaginal or rectal intercourse. this is based on the following findings: a) elevated titers of anti-ct igg are present in symptomatic acquired immunodeficiency syndrome (aids) patients; b) elevated titers of anti-ct iga are found in asymptomatic aids patients; c) low anti-ct iga titers were observed during the progression o ... | 1992 | 1501589 |
| medicine and life. | | 1992 | 1502542 |
| the long term follow-up of asymptomatic women with chlamydia trachomatis. | a community oriented intervention program was initiated in 1985 in 12 rural communities in southern israel to identify and treat women of reproductive age with markers of chlamydia trachomatis (ct) infection. among 860 women tested, 21 (2.4%) had ct igg antibody titers greater than or equal to 128, or ct iga antibody titers greater than or equal to 16, and 9 of these women had positive cultures for ct. the 21 women, as well as their male partners, received specific anti-ct treatment, and were fo ... | 1992 | 1503506 |
| the new macrolides: expanding the ways in antibiotic treatment. | after almost forty years of its introduction, erythromycin will not be the exclusive member of the macrolide group of antibiotic agents, but a new generation of its derivatives which surpass it in pharmacological properties and clinical efficacy will also be available. clarithromycin, a 14-membered derivative, has shown acid stability, longer half-life, lower protein binding and higher lung tissue penetration. its exceedingly high activity against erythromycin-susceptible gram-positive cocci, my ... | 1992 | 1503585 |
| role of chlamydia trachomatis infections in infertility due to tubal factor. | the prevalence of igg antibodies to c. trachomatis was determined in 185 infertile women who underwent laparoscopy, and 110 pregnant women with no known infertility problems. in addition, chlamydial antigens were evaluated by eia in cervical samples taken from all subjects. subjects with tubal infertility had the highest prevalence of chlamydial antigen and antibody (p less than 0.01). while the percentage of subjects with antigen and antibody positive was 11.6 per cent, those with antigen negat ... | 1992 | 1506064 |
| screening for chlamydia trachomatis in college women on routine gynecological exams. | chlamydia trachomatis infection poses a serious threat to sexually active college women. past research has demonstrated the difficulty of predicting infection on the basis of symptoms or physical exam. recent studies highlight the prevalence of asymptomatic chlamydia infections. this study presents the results of screening 2,303 women for chlamydia infection on routine annual gynecological examinations at a college health center during a 22-month period. a prevalence of 6% was found. the study a ... | 1992 | 1506565 |
| [detection of chlamydia trachomatis in abdominal cavity using nested pcr]. | | 1992 | 1506737 |
| sexually transmitted diseases and child abuse. | the diagnosis in a child of any infection that is usually sexually transmitted in adults should raise the suspicion of possible sexual abuse. we know sexual abuse is a common childhood experience. the american academy of pediatrics committee on child abuse and neglect has rated the implications of sexually transmitted diseases for diagnosing sexual abuse (table 6). | 1992 | 1508576 |
| [contact tracing in genital chlamydia infections]. | only one in five general practitioners in norway initiates notification of partner(s) after diagnosing a case of genital chlamydial infection. in order to increase the use of this efficient case-finding method we review the aims of notifying partners and the judicial, ethical and economical aspects of the method. we describe both provider-referral and patient-referral methods. the first approach is chosen when the index patient wants to remain anonymous. general practitioners are advised not to ... | 1992 | 1509433 |