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effect of induced thrombocytopenia on experimental circulatory shock. 19761011824
antibodies to two immunotypes of chlamydia trachomatis in individuals with trachoma.in tunisia (north africa), trachoma remains a common eye disease. most cases are caused by immunotype a of chlamydia trachomatis. in a small proportion of cases (less than 10%), type-specific antibodies to both immunotype a and immunotype b exist in the serum of patients. these types do not crossreact, and occasionally sequential acquisition of antibodies has been demonstrated. thus, individuals in one endemic area of trachoma may be infected with two immunotypes of c. trachomatis. the epidemiol ...1976971956
infectivity titration of guinea-pig inclusion conjunctivitis agent in irradiated mccoy cells.estimation of the infectivity of the agent of guinea-pig inclusion conjunctivitis for irradiated mccoy cells, assayed as inclusion-forming units, was influenced by the age of cells after irradiation, the maturation time of the inclusions, the centrifugal force and the centrifugation temperature. agent passaged through irradiated mccoy cells or guinea-pig conjunctivae showed a greater capacity to infect irradiated mccoy cells without centrifuging than agent grown in a chick embryo. the nature of ...1976986419
thyroid-stimulating hormone and thyrocalcitonin content in rat thyroid. 19761011825
amylase clearance in acute pancreatitis experimental study in the guinea pig. 19761011827
the involvement of the electron transport chain in uptake of testosterone by membrane vesicles of pseudomonas testosteroni. 19761011843
the lack of effect of ampicillin plus probenecid given for genital infections with neisseria gonorrhoeae on associated infections with chlamydia trachomatis.forty-six men were successfully treated with a single oral dose of ampicillin (2 g) plus probenecid (1 g) for urethral infections with neisseria gonorrhoeae. chlamydia trachomatis was isolated from cultures of cells obtained from 11 of these men both before and after treatment; c. trachomatis was isolated from one man before but not after treatment and from three men after but not before treatment. no isolates were obtained from the remaining 31 men either before or after treatment. of the 15 pa ...1976816978
the owl monkey (aotus trivirgatus) as an animal model in trachoma research.the owl monkey, aotus trivirgatus, in contrast to other species of nonhuman primates, was shown to be susceptable to infection with strains of chlamydia trachomatis. it responded to experimental infection with a severe, acute, inflammatory reaction of short duration. monkeys which recovered from infection were resistant to reinfection for more than 6 months and resistance was not strain specific. eye secretion antibody titers correlated well with resistance to subsequent challenge. these studies ...1976828229
simplified serological test for antibodies to chlamydia trachomatis.three-hundred sixty sera from unselected patients attending two london venereal disease clinics were examined by a microimmunofluorescence test. eleven egg-grown serotypes of chlamydia trachomatis and the so-called "fast" strain sa2(f) were used as antigens. of the 360 sera tested, 119 (33%) reacted to a titer of 1:16 or above with at least one antigen. of these positive sera, over 50% cross-reacted with all 12 serotypes, and 95.5% reacted with sa2(f) in addition to other antigenic types. it is ...1976783191
inclusion blennorrhea: a case report.the case described here represents the first laboratory-confirmed case of inclusion blennorrhea at the mayo clinic since laboratory isolation of chlamydia trachomatis was instituted in 1974. treatment with a sulfonamide preparation proved effective. further aspects of the diagnosis and treatment of inclusion blennorrhea are discussed.1976785121
chlamydial infection of the male urethra.urethral specimens from 477 men were collected with endourethral swabs and examined for chlamydia trachomatis by cell culture on mccoy cells pretreated with idoxuridine. of these men, 141 had gonococcal urethritis, 262 non-gonococcal urethritis (ngu), and 74 showed no evidence of urethritis. of 118 men with heterosexually acquired gonococcal urethritis, thirty (25 per cent.), and of 23 men with homosexually acquired gonococcal urethritis, five (22 per cent.), yielded c. trachomatis from the uret ...1976816416
non-specific urethritis. a placebo-controlled trial of minocycline in conjunction with laboratory investigations.the results of a double-blind therapeutic trial on 81 men suffering from non-specific urethritis (nsu) show that minocycline was more effective than a placebo. before treatment chlamydia trachomatis was isolated from 31 per cent. of the men, ureaplasmas from 58 per cent., and mycoplasma hominis from 7-5 per cent. there is evidence that chlamydiae are a cause of urethritis. however, after minocycline therapy improvement in the clinical response of patients from whom only ureaplasmas were isolated ...1976786440
genital chlamydial infections.the isolation of chlamydial trachomatis in sera of patients with gon ococcal urethritis (gu), non-gonococcal urethritis (ngu), post-gonococca l urethritis (pgu) and trachomainclusion conjunctivitis (tric) is discussed. chlamydiae were isolated in 40% of men with ngu, 20-30% with gu and 0-7% of men with symptomatic or microscopic evidence of urethritis. the rates of isolation of chlamydiae in ngu patients were significantly higher than in controls (p less than .001). the sexual tr ansmitabilit ...1976775980
a method for testing the antibiotic susceptibility of chlamydia trachomatis in a cell culture system. 1976931835
characterization of miniature pig kidney cells and their resistance to chlamydial infection.a miniature pig kidney cell line has been established from porcine fetuses taken aseptically by hysterectomy and maintained for more than 50 passages in eagle's minimal essential medium containing 10% heat-inactivated newborn calf serum. cell transfers were performed each week. primary and serially passaged cells were found to be highly refractory to infection by chlamydia trachomatis strains tw-3, bour, and lgv 440l and chlamydia psittaci strains meningopneumonitis and 6bc and insusceptible to ...1976944002
the inactivation of chlamydia trachomatis by povidone-iodine. 1976945260
differences in physicochemical and antigenic properties of chlamydial strains.antigenic analysis of chlamydia psittaci, c. trachomatis, and lymphogranuloma venereum (lgv) revealed basic physicochemical differences among the three chlamydial strains. these were manifested in structural, isoelectric points, absorption spectra, and in the characteristics of the chromophobe-containing proteins. the effective period of sonication for c. psittaci and c. trachomatis is around 60 min during which the linkages most susceptible to external sonication forces were broken, releasing a ...1976963615
experimental study of the antitrachoma action of berberine. 1976992845
analytical subfractionation of microsomal fractions from the livers of control and gunn-strain rats. 19761001660
mediation of cytotoxic immune responses against human tumor-associated antigens by xenogeneic immune rna.xenogeneic immune rna (i-rna), extracted from the lymphoid organs of sheep or guinea pigs immunized with human tumor cells, mediated in vitro cytotoxic immune responses that were directed specifically against tumor-associated antigens of human tumor target cells. normal human peripheral blood lymphocytes from healthy donors became markedly more cytotoxic for human tumor target cells after being incubated with i-rna extracted from the lymphoid organs of animals that had been immunized with that p ...19761003510
isolation of chlamydia trachomatis from women attending a clinic for sexually transmitted diseases.attempts were made to isolate chlamydia trachomatis from the cervix of 300 women attending a clinic for sexually transmitted diseases in leeds. the women were divided into four groups; (1) 130 were consorts of men suffering from non-specific urethritis; (2) 66 were suffering from gonorrhoea, or were consorts of men suffering from this disease; (3) 56 were suffering from other sexually transmitted diseases; (4) 48 had no evidence of std. the overall isolation rate of chlamydia trachomatis was 20% ...19761009420
lymphocyte locomotion iii. ultrastructural studies of the lymphocyte traffic over the postcapillary venules of rat lymph nodes.the direction of lymphocytes with amoeboid movement configuration (amc) was evaluated by means of electron microscopy of ultrathin sections of post-capillary high-endothelium venules (he-venules) from rat lymph nodes. out of 104 lymphocytes, 68 lymphocytes appeared to be on their way towards the lumen of the he-venule and 36 lymphocytes appeared to be moving away from the venular lumen at the moment of fixation. this difference, which was statistically significant (p=0.0024), is thought to refle ...19761011862
the microcirculation of the mammalian lymph node.flow alterations to give complete filling of the lymphatic sinusoidal system and saccular lymph spaces around the germinal centers were demonstrated during a primary immune reaction. by contrast, in delayed hypersensitivity, saccules were not seen although there was marked enlargement of individual follicular units.19761011863
metaphyseal osteopathy (hypertrophic osteodystrophy) in growing dogs. a clinical study. 19761011799
[relationship of the biological effect of radiation to the area of irradiated body surface].the dependece of radiation injury of piglets on the area of irradiated body surface (v 40 kv, eeff 17 key, 1 10 ma per tube, additional filter 0.1 mm a1, delta 1/2 0.08 mm a1, source--skin distance 30 cm, r 504 r/min, radiattion dose 200 r) was studied. the biological effect was evaluated with respect to the clinical, hematological and biochemical changes in the animal body. the data were obtained that indicated burn disease in the irradiated piglets, severity of which increased with an increase ...19761011787
[antiviral effects of acridine derivative c 283 in herpes simplex studies]. 1976176541
effect of zinc ions on synthesis of herpes simplex virus type 2-induced polypeptides. 1976181763
oxidative phosphorylation in micrococcus denitrificans: calculation of the p/o ratio in growing cells.p/o ratios were measured in membrane particles obtained from cells of micrococcus denitrificans, while growing on different carbon sources. the membrane particles obtained from cells growing actively on glucose, succinate, ethanol and propanol as the carbon and energy sources catalyzed oxidative phosphorylation and yielded respective p/o ratios of 1.4, 1.2, 0.8, and 0.5 with nadh, and 0.8, 0.6, 0.6, and 0.5 with succinate as the electron donors. not such a difference in p/o ratio is observed in ...1976179486
studies on the corticotrophin-releasing activity of vasopressin, using acth secretion by cultured rat adenohypophyseal cells.crf activity of synthetic vasopressins and pitressin was studied in an in vitro system of cultured rat adenohypophyseal cells using direct measurement of acth by radioimmunoassay. pitressin (posterior pituitary extract) induced a dose-related secretion of acth whereas synthetic arginine or lysine vasopressin were devoid of crf activity, even with the largest tested dose (4 mug/ml). no potentiation of the crf activity of hypothalamic extract was observed with any vasopressin preparation studied. ...1976179790
daily variation in concentration of plasma corticosteroid in hypophysectomized rats.a daily variation in the concentration of plasma corticosteroid hormone is present in hypophysectomized rats implanted with pellets of adrenocorticotropic hormone (acth) and thyroxine. although acth is necessary to maintain a functional adrenal, and thyroxine is required to permit the expression of the plasma corticosteroid rhythm, a normal daily variation in plasma corticosteroid concentration does not appear to depend on the periodic release of pituitary hormones.1976179796
reduction of 5'-nucleotidase activity in rat thyroid and adenohypophysis following methylthiouracil treatment.5' -nucleotidase activity was determined in rat thyroid and some other organs employing a specific assay method. during the course of methylthiouracil (mtu) treatment, thyroid 5'-nucleotidase activity decreased significantly. this decrease was specific for this enzyme since the activity of neutral phosphatase did not change and the activity of alkaline phosphatase and mg2+-activated adenosine triphosphatase increased markedly. the 5'-nucleotidase activity of the adenohypophysis also decreased fo ...1976179798
a noradren aline sensitive adenylate cyclase in the rat limbic forebrain: preparation, properties and the effects of agonists, adrenolytics and neuroleptic drugs.a method for the preparation of a noradrenaline sensitive adenylate cyclase from homogenates of the rat 'limbic' forebrain is described using krebs--ringer as the homogenising medium. some of its properties resemble those reported previously by other workers, using slices. its response to agonists show that it has the characteristics of a beta1-receptor i.e. the potency of 1-isoprenaline exceeds that of 1-noradrenaline which exceeds that of 1-adrenaline. structure--activity analysis of the respo ...1976179826
immunofluorescent detection of adenovirus antigen in epidemic keratoconjunctivitis.an immunofluorescent technique was used to demonstrate soluble adenoviral antigens in epithelial cells on conjunctival scrapings of patients with epidemic keratoconjunctivitis (ekc). conjunctival scrapings for immunofluorescence and viral isolation studies were performed on 79 patients suspected of having ekc or other acute follicular or papillary conjunctivitides. of 41 patients with clinical findings consistent with a diagnosis of ekc and three patients with pharyngoconjunctional fever, 43 wer ...1976176130
relationships of foreign protein injections (hyposensitization) in atopic children to serum lipids and lipoproteins.foreign protein injections (hyposensitization) given for one year to human asymptomatic asthmatics did not increase their serum total lipids or lipoprotein levels, as they apparently did in rabbits fed a lipid-rich, cholesterol-poor diet. serum total protein and immunoglobulin levels for iga, igm and igg for the asthmatics and controls were no different.1976174460
effect of ambient temperatures on multiplication of attenuated transmissible gastroenteritis virus in the bodies of newborn piglets.newborn piglets were found to be more resistant to infection with attenuated transmissible gastroenteritis virus when maintained at higher temperatures. this was attributed to a decreased rate of virus propagation and spreading in the bodies of the infected animals. the highest virus levels were detected in the tissues of piglets maintained at 8 to 12 c. in contrast, no virus was recovered from piglets maintained at 35 to 37.5 c. the virus was found only in the lymph nodes and respiratory organs ...1976178601
modified hemolytic plaque technique for the detection of bluetongue virus antibody-forming cells.a hemolytic plaque assay was developed for the detection of antibody-forming cells to bluetongue virus (btv). sheep erythrocytes (srbc), onto which btv had been absorbed, served as the indicator of lysis due to the presence of btv antibody-forming cells. the ratio of btv to srbc was found to be critical for optimum hemolytic plaque formation. for routine use, 50 mul of 12% btv srbc, 0.1 ml of a spleen cell suspension, and 0.5 ml of 0.5% agarose in a balanced salt solution were mixed and plated o ...1976178602
localization of selenium in bacterial cells using tem and energy dispersive x-ray analysis.bacteria isolated from lake sediment samples reduced sodium selenite to elemental selenium. finestructural observations were made on a number of different bacterial species cultured in the presence of sodium selenite. examination of escherichia coli and a pseudomonas species revealed electron-dense deposits of irregular shape, composed of smaller units, within the cytoplasm but not on the cell wall and cell membrane. cells of aeromonas and flavobacterium species exhibited conspicuous intranuclea ...1976175749
inhibition of lymphocyte blastogenesis by factor(s) in alloxan-diabetic rat plasma.supplementation of rat lymphocyte cultures with plasma from alloxan-diabetic rats produced a dose-dependent suppression of mitogen-induced blastogenic responses. viability measurements indicated that this inhibition was not due to a direct cytotoxic effect of alloxan-diabetic plasma on rat mononuclear leukocytes in vitro. this inhibition was not explained by hyperglycemia alone and was observed when blastogenesis was induced in vitro by phytohemagglutinin (pha), concanavalin a (con a), or alloge ...1976179906
interaction of chlamydia trachomatis organisms and hela 229 cells.the infection of hela 229 cells in monolayer culture with trachoma (b/tw-5/ot) and lymphogranuloma venereum (lgv) (l2/434/bu) organism was studied in terms of two parameters: radioactivity counts of cell-associated tritium labeled organisms at the initial stage of inoculation for measurement of attachment, and inclusion counts of infection cells after incubation for measurement of growth. factors affecting attachment and inclusion formation and correlation of the two are presented. it was shown ...1976179950
sensitivity of rhinoviruses to human leukocyte and fibroblast interferons.rhinoviruses were tested for sensitivity to human interferon from two sources: human leukosytes induced with sendai virus and human diploid fibroblasts induced with polyriboinosinic-polyribocytidylic acid. twenty-five serotypes of rhinovirus were tested against fibroblast interferon in hela cells, and five serotypes were evaluated in fibroblast cultures against fibroblast and leukocyte interferons. sensitivity varied widely in the hela cell assay; rhinovirus type 10 was inhibited by approximatel ...1976180194
[differences in the enzymatic activity of mitochondria from enriched neuronal and glial fractions].studies were performed in the activities of certain enzymes from oxidoreductase group: cytochrome c-oxidoreductase (ec 1.6.99.3), succinate dehydrogenase succinates: cytochrome c-oxidoreductase (ec 1.3.99.1), cytochrome oxidase (ec 1.9.3.1) and malate dehydrogenase (ec 1.1.1.37) in mitochondria from neuronal and glial-enriched fractions. the mitochondrial fraction purity was observed by the electron microscope. the enzyme activity of the glial mitochondrial fraction was much higher than that in ...1976181884
properties and partial purification of an oestrogen receptor from neonatal rat brain. 1976182324
standardization fo a digestion-filtration method for isolation of pancreatic islets.standardization of a technic for isolating large numbers of pancreatic islets is described. this procedure employed collagenase digestion of rat pancreatic tissue in a cylindrical wire screen in order to separate isolated islets from undigested pancreas. from this basic protocol the following conditions were established: (1) the duration of the initial digestion period was found to be optimal at six minutes; (2) three subsequent digestions of one minute each effected maximum islet yield; (3) the ...1976182606
relative responsiveness (rho): a critical analysis of a new method in receptor differentiation.the rho ratio, which measures the capability of a receptor system to induce a maximal contraction after saturation by a full agonist was analysed from the standpoint of its variability considering the values obtained either for a given receptor system in the same type of preparation or for different types of preparation. this variability was discussed on the grounds of receptor theory, as the basis for a new method in receptor differentiation.1976185120
regulation of sterol synthesis in adrenal gland of the rat by both high and low density human plasma lipoproteins. 1976186062
effect of anti-lymphotoxin on cell-mediated cytotoxicity. evidence for two pathways, one involving lymphotoxin and the other requiring intimate contact between the plasma membranes of killer and target cells. 1976186197
effects of 5-bromodeoxyuridine on the acth-dependent mitochondrial biogenesis in cortical cells of fetal rat adrenals in tissue culture.cortical cells of fetal rat adrenals in tissue culture were treated with 5-bromodeoxyuridine (brdu) during their proliferative phase and during acth stimulation when nuclear dna synthesis has almost ceased. pretreatment with 0.5 mug/ml/day of brdu inhibited the acth-induced differentiation of cortical cells as well as the secretion of corticosterone and 18-oh-deoxycorticosterone (18-ohdoc). when nuclear dna synthesis was suppressed and mitochondrial dna synthesis was stimulated by acth brdu addi ...1976186465
quantitative high-performance liquid chromatography of diazepam and n-desmethyldiazepam in blood.high-performance liquid chromatography on porous silica has been employed to determine diazepam and n-desmethyldiazepam in human blood. for forensic purposes, 1.0 ml of blood is sufficient for a quantitative determination of the benzodiazepines in concentrations above 100 ng/ml. in cases where lower levels, 25-100 ng/ml, are of interest, 2.0 ml of blood together with a somewhat more elaborate extraction procedure are necessary.1976186471
the release of endogenous amino acids from the rat visual cortex.the release of endogenous taurine, gaba, glycine, aspartate, glutamate, glutamine and alanine from the rat visual cortex was measured using a cortical cup technique. the electrocorticogram (ecog) was monitored throughout most experiments. 2. spreading depression, evoked by the dropwise placement of 10% kcl solution on to the brain outside the cup was associated with a significant increase in the release of gaba and glutamine but a marked fall in that of glutamate. the evoked release of gaba and ...1976186588
eeg and behavioral effects of morphine in dogs. 1976186809
isolation and localization of rna fractions able to transfer tumor-specific delayed hypersensitivity in vitro.rna fractions were prepared by sucrose density gradient centrifugation from hot-cold phenol, rna-rich extracts of lymphoid tissues from strain 2 guinea pigs hyperimmunized to line 1 or line 10 tumors. each rna fraction was assessed for its ability to convert nonsensitized strain 2 peritoneal exudate cells to a state of specific sensitivity for line 1- or line 10-solubilized tumor antigens. an rna fraction residing between the 4 s and 18 s peaks, designated as fraction "b", transferred line 10 or ...1976187327
interrelationships between the central nervous system and patterns of adrenocorticotropic secretion following acute exposure to severe environmental conditions. 1976187557
purification and properties of an endonuclease from nuclei of uninfected and polyoma-infected 3t3 cells.an endonuclease activity has been purified approximately 800-fold from nuclei of 3t3 cells infected with polyoma virus. the purfied enzyme catalyzes an endonucleoytic cleavage of single- and double-stranded dna and single-stranded rna. evidence that the activity towards these substrates resides in the same protein molecule is provided by the finding that they co-sediment in sucrose gradients and have identical rates of heat inactivation. studies on the dnase activity shows that the rate of hydro ...1976187596
new evidence for the activation of factor xii by epinephrine. 1976180627
low-density beta-lipoproteins (ldl) in swine: investigations of linkage relationships. 1976188358
[burkitt's lymphoma (author's transl)].burkitt's lymphoma occurs endemically in central africa, but is also found sporadically in areas with temperate climate. a case of burkitt's lymphoma found in our area is described. the literature in regard to the clinical symptoms, etiology and treatment of burkitt's lymphoma is reviewed. at the present time cyclophosphamide is considered to be the drug of choice; it leads to a remission rate of about 90%.1976180474
acceptor proteins in rat androgenic tissue chromatin.fractionation of chromatin into urea-soluble chromosomal nonhistone proteins (up), histones (hp), and dna-associated nonhistone proteins (np) revealed that the np fraction from testicular and prostatic chromatin contains organ-specific acceptors for complexes of 5alpha-dihydrotestosterone (17beta-hydroxy-5alpha-androstan-3-one) and its receptor. this acceptor capacity of androgenic tissue chromatin could be transferred to chromatins from non-target tissues with the np fraction of dna-associated ...1976180532
nicotinamide nucleotide transhydrogenase in entamoeba histolytica, a protozoan lacking mitochondria. 1976175989
susceptibility of beta-hemolytic streptococci to 65 antibacterial agents.tests for susceptibility of 29 group a, 4 group c, and 2 group g strains of beta-hemolytic streptococci to 63 antibiotics and to trimethoprim and sulfamethoxazole, singly and combined in a ratio of 1:16, were carried out in vitro. all strains tested were moderately or highly susceptible to all the antibiotics used except those belonging to the aminoglycoside and polymyxin groups. a few were also resistant to the tetracyclines and to sulfamethoxazole alone. comparisons with results obtained in pr ...1976176926
cross-linking studies on the state of association of apo a-i and apo a-ii from human hdl. 1976183777
the human tongue during sleep: electromyographic activity of the genioglossus muscle. 1976177304
detection and characterization of differences in plasma corticoid response to treatments.ten experiments involving 3013 observations and 103 animals were studied to characterize variation associated with bovine plasma corticoid concentrations (ng/ml). total corticoids, corticosterone, or cortisol were measured by competitve protein binding assay. animals were noncystic or cystic, and measurements were made to establish basal concentrations or to estimate response to stimulation by adrenocorticotrophic hormone injection. data were analyzed by least squares with the nested model: trea ...1976177471
dopamine synthesis in rat brain striatal synaptosomes. ii. dibutyryl cyclic adenosine 3':5'-monophosphoric acid and 6-methyltetrahydropterine-induced synthesis increases without an increase in endogenous dopamine release.the effects of n6, o2'-dibutyryl cyclic adenosine 3':5'-monophosphoric acid (dibutyryl cyclic amp), dl-6-methyl-5, 6, 7, 8-tetrahydropterine (6-mph4) and tyramine on dopamine synthesis and endogenous dopamine release in rat brain striatal synaptosomes have been examined. dibutyryl cyclic amp (1 mm) produced a 100% increase in dopamine synthesis but had no effect on endogenous dopamine release. veratridine (75 mum) did not further stimulate dibutyryl cyclic amp-treated tissue. 6-mph4 (0.1 mm) als ...1976177758
a comparative morphometric analysis of the effects of thyroxine and acth on the zona fasciculata of rat adrenal cortex.the effects of thyroxine and acth on the ultrastructure of cells of the adrenal zona fasciculata were studied by morphometric analysis and compared by two-way analysis of variance. the results indicate that the effects of thyroxine and acth are different and independent. the statistically significant differences in effect of thyroxine and acth are: thyroxine causes an increase in the volume fraction of mitochondria and a decrease in the volume fraction of endoplasmic reticulum independent of act ...1976178165
gastric mucosal blood flow and pepsin secretion in dogs--stimulation by 13-nle-motilin.in response to graded doses of intravenous 13-norleucine-motilin (13-nle-motilin)--a synthetic analogue of motilin and biologically equivalent to the natural polypeptide-, gastric mucosal blood flow (gmbf) in canine vagally denervated fundic pouches was studied using the aminopyrine clearance technique. as 13-nle-motilin did not exert any detectable effect on gastric secretion of hydrogen ions, intraluminal instillation of 160 mm hcl was used to provide a ph gradient allowing aminopyrine to move ...1976175432
the specificity of anti-actin serum.the immunogenicity of smooth muscle actin is increased by ageing at 4 degrees for at least a week. rabbits lacking natural smooth muscle antibodies were injected with 1 mg of aged purified actin in adjuvant. fourteen out of thirty-six rabbits produced serum antibodies which precipitated with actin solution, but not with smooth muscle tropomyosin, myosin, light or heavy meromyosin or with other unidentified non-actin proteins in crude extracts. analysis of crude actin extract before and after pre ...1976135724
induction of smon-type virus form avian infectious laryngotracheitis virus.smon-type virus which shows different host range was induced from ilt virus under unsuitable culture conditions for the growth of the parental ilt virus, such as cultivation in cam of 12-day-old eggs or cultivation in chk cells at 32 degrees. furthermore, it was induced from ilt virus by mutagenesis by either brdu or ng in chk cell cultures.1976175380
arterial metabolism in primates. 1976174070
similarities of the golgi apparatus membrane and the plasma membrane in rat liver cells.a golgi apparatus-rich fraction and a plasma membrane-rich fraction were isolated from a common homogenate of rat liver. their respective buovant densities, appearances in the electron microscope and 5'-nucleotidase and udp-galactose ovalbumin galactosyltransferase activities were in accord with published data on separately isolated golgi apparatus-rich and plasma membrane-rich fractions. contamination by endoplasmic reticulum and mitochondria was low. gel electrophoresis of the membrane protein ...1976175074
the macromolecular organization of the elastin fibril. 1976175164
anti-viral activity of single-stranded homopolynucleotides against encephalomyocarditis virus and semliki forest virus in adult mice without interferon induction.single administrations of poly c or poly i are anti-viral against infections of encephalomyocarditis (emc) and semliki forest virus (sfv) in mice but poly u and poly a are not. the degree of protection is dose-dependent and mice which die do so at a later time when untreated controls even in a strain of mouse in which the time of death is not dependent on the dose of virus given. no circulating interferon is found after treating mice with poly c or poly i even at polynucleotide doses which give ...1976173799
cytopathic effects in primary epithelial cultures derived from the human prostate.normal, benign, and malignant human prostatic tissue was cultivated in vitro. cytopathic effects in derived epithelial cells were examined. light microscopy revealed polykaryocyte formation, vacuolation, cytoplasmic bridges and processes, nuclear inclusions, increased acid phosphatase activity at the cell membrane of polykaryocytes as compared to mononuclear cells, and cell rounding and clumping. electron microscopy of the polykaryocytes showed nuclear membrane proliferation and protrusion, scar ...1976173669
fractionated precipitation of acid macropolyanions by dialysis, a simple method for the estimation of dna in complex biological samples.after efficient extraction by para-aminosalicylate, chopping, grinding and eventual sonication, the macropolyanions are transformed into their cetyltrimethylammonium salts. these have differing solubilities, strongly depending on ionic strength. the cationic detergent-macropolyanionic salts are solubilized by high salt concentration. salt is then dialysed out, rendering the polyanions highly insoluble in a sequential fashion. the insolubilized components are determined quantitatively by monitori ...1976134556
[research on the inhibitors of cell proliferation of the "chalone" type in the rat liver at the cancer institute of montreal]. 1976134483
vagal afferents essential for abdominal muscle activity during lung inflation in cats.maintained inflation of the lung evokes abdominal muscle activity in anesthetized cats only if the vagus nerves are intact, indicating the importance of vagal receptors. the location of these receptors was determined in 14 anesthetized cats by comparing prevagotomy inflation responses of the abdominal muscles and diaphragm to the responses obtained after section of the thoracic vagi at one of three different levels. the abdominal muscle and diaphragm responses to maintained lung inflation persis ...1976134985
studies of the histochemistry, ultrastructure, motor innervation, and regeneration of mammalian intrafusal muscle fibres. 1976137430
pharmacological experiments as a basis for the administration of digoxin in the horse.it is shown that the concentration of ouabain necessary for 50 per cent inhibition of the na+k activated membrane atpase of red cells is similar in man and horse. this is taken to indicate that the two species have similar sensitivity towards cardiac glycosides in general. in five adult healthy horses plasma digoxin concentration was measured with a radioimmunoassay technique after a single intravenous injection of 1 mg/100 kg body weight digoxin. the half time of elimination was 23 h and the ap ...1976130670
prevention of vascular complications of diabetes by pancreatic islet transplantation.isolated pancreatic islets from wistar-lewis rats were transplanted into the liver of diabetic allogeneic recipients to assess ability to prevent diabetic renal and ophthalmic complications. at nine months, the diabetic animals without transplants showed significant increase in pas-positive material in the renal glomerular mesangium and thickening of glomerular arterioles as compared with normal nondiabetic animals. new vessel formation was also significant in the retina and retinal capillary di ...1976130850
a search for mycoplasmas and chlamydiae in acne lesions.no mycoplasmas and no chlamydiae were found in cultures of 75 uninflamed comedones, 72 papulopustular lesions, and 7 cystic lesions from 14 subjects with acne. chlamydial serologic tests were negative for 11 subjects and showed antibodies in 3 subjects to 3 different antigenic types of trachoma agent. these results are viewed as substantial evidence against the possibility that either mycoplasmas or chlamydiae play an important part in the pathogenesis of acne.1976133192
[possibilities of correcting some metabolic disorders in experimental endotoxic shock by treatment with a combination of alpha-lipoic acid and epsilon aminocaproic acid].alpha-lipoic acid associated with xi-aminocaproic acid proves to have a protector effect in the posttreatment of endotoxinic shock in rabbits, produced by the administration of e. coli o111, in doses of 1.5 mg/kg body weight. this effect was observed when administered early in the stage of energy privation, and was manifested by a longer survival, diminution of lactacidemia and protection of labile phosphate reserves.1976133444
evidence for immune complexes involving anti-lymphocyte antibodies associated with hypocomplementaemia in chronic lymphocytic leukaemia (cll).unmeasurable total haemolytic complement (c) was observed in serum of a patient with untreated chronic lymphocytic leukaemia and recurrent non-hereditary angioedema. analysis of c components immunochemically demonstrated a marked reduction of c1q and c1s inhibitor, undetectable c1r, c1s and an elevated b. haemolytic c1, c4 and c2 were less than 5 percent of normal, functional c1s inhibitor was absent. cryoglobulin and c1q precipitins were present in the serum. of special interest was the presenc ...1976136325
fibroblast dna synthesis activation in sponge induced granulation tissue. the effect of antineutrophil serum and cyclophosphamide.dna synthesis in rat and rabbit polyvinyl sponge induced granulation tissue has been studied using thymidine (methyl-3h). synthesis was determined by measurement of thymidine incorporation into cold trichloroacetic acid insoluble material and by autoradiography. granulation tissue was removed and immediately incubated in vitro in the presence of thymidine (methyl-3h) for three hours. the label was incorporated into the nuclei of fibroblasts and, to a lesser extent, of endothelial cells. the labe ...1976136330
[significance of serum digoxin concentration and its influencing factors].in recent years it has become possible by means of a radioimmunoassay to measure digoxin concentration in the serum of digitalized patients. with this method it could be shown that the resorption of digoxin is decreased by partial resection of the samll intestines, by malabsorption syndromes, after ingestion of neomycin, colestyramine and antacids. in renal insufficiency, however, the elimination half-life period of digoxin is increased conspiciously (from about 35 hours up to about 120 hours). ...197661153
letter: growth of chlamydia trachomatis in cell culture. 197657430
a serial study of pregnancy proteins in primigravidae.the plasma concentrations from four 'pregnancy proteins' and three steroid hormones have been measured throughout pregnancy in 15 primigravidae. two of the proteins, human placental lactogen (hpl) and pregnancy-specific beta1-glycoprotein (psbetag), are specific for pregnancy and correlate well with the stage of gestation. it is suggested that measurement of psbetag may be useful in assessing feto-placental wellbeing. neither of the pregnancy-associated proteins, steriod-binding beta-globulin (s ...197657799
[identification of the causative agents of trachoma and paratrachoma by the method of direct immunofluorescence and staining by the romanovskiÄ­-giemsa method]. 197656498
evidence for more than one ia antigenic specificity on molecules determined by the i-a subregion of the mouse major histocompatibility complex.ia antigenic specificities determined by the i-a subregion of the mouse major histocompatibility complex have been examined in strain b10.d2 (h-2d), c57bl/10 (h-2b), and in a (c57bl/6xdba/2) hybrid (bdf1; h-2b/d). detergent solubilized, 3h-leucine-labeled antigen preparations were mixed with appropriate alloantisera and precipitation was induced either by addition of goat anti-mouse gamma-globulin or by addition of protein a-bearing staphylococci. sequential precipitation analysis showed that in ...197655444
anatomical organization of the phasic activity produced by reserpine at the level of the oculomotor system.the organization of the pathways responsible for the transmission of phasic electrical activity at the level of the oculomotor system was studied in the encéphale isolé cat which was injected with reserpine or exhibited spontaneous phases of paradoxical sleep. at the level of the vith nuclei there are both ipsilateral and contralateral connections deriving from each generator. the ipsilateral pathway transmits an "inhibiting" potential eliciting electromyographic inhibition of the ipsilateral re ...197655344
cellular and humoral immunity in viral hepatitis.cellular and humoral immunity combine to determine the outcome following exposure to hepatitis virus and are implicated in the proposed pathogenetic mechanism for acute and chronic hepatitis. although antibody to hbsag is found in virtually all following recovery from hepatitis b, a cell-mediated response to hbsag can be detected in most patients during the acute phase, and it has been suggested that this may cause the acute hepatic damage by an attack on virus-infected cells. patients who have ...197685410
fetal syphilis in the first trimester.evidenc of first-trimester fetal syphilis was sought in the products of conception in a therapeutic abortion clinic. during two collection periods of one week, five patients with serologic and clinical data consistent with recent syphilitic infection were identified. their conceptuses were carefully examined by silver and immunofluorescent stains for the presence of treponema pallidum. two of these five conceptuses (9 and 10 weeks' gestation) were found to contain t. pallidum by these methods. t ...197656895
letter: growth of chlamydia trachomatis in cell culture. 197656688
biochemical and behavioral changes in rats exposed to ethanol in utero. 197618974
isolation of chlamydia trachomatis in tissue-culture of human thyroid. 197661387
differential response of chlamydial and ureaplasma-associated urethritis to sulphafurazole (sulfisoxazole) and aminocyclitols.91 men with non-gonococcal urethritis (n.g.u.) were randomly treated with either sulphafurazole (sulfisoxazole), 500 mg orally q.i.d. for 10 days, or an aminocyclitol (streptomycin or spectinomycin), 2 g intramuscularity for 1 to 3 doses at 12 h intervals. initial urethral cultures were positive for chlamydia trachomatis (c) in 36 (40%). ureaplasma urealyticum (u) was isolated from the urethra or urine from20 (95%) of 21 white men in a first episode of n.g.u. who had negative chlamydia cultures. ...197663748
[the effect of increased intracranial pressure and subarachnoid hemorrhage on the sympathetic vasoconstrictor nerve activity (author's transl)]. 197664942
comparison of chlamydia subgroup a detection from clinical specimens after 40 and 64 hours of incubation in 5-iodo-2-deoxyuridine-treated mccoy's cells.the time course of formation of inclusion bodies produced by chlamydia in 5-iodo-2-deoxyuridine (iudr)-treated mccoy's cells was studied with the use of a known isolate of chlamydia trachomatis d/uw-184/ur and 47 frozen clinical urethral specimens previously shown to be either positive or negative for chlamydial inclusions after 3 days of incubation. subsequent examination of 369 clinical specimens from the genitourinary tract over a 6-month period revealed 47 (13%) chlamydia-positive cultures, ...197657968
effects of poly(1-vinyluracil) and poly(9-vinyladenine) on viral rna-directed dna polymerase.the effects of poly(1-vinyluracil) [poly(vu)] and poly(9-vinyladenine) [poly(va)] on the rna-dependent dna polymerase activity of murine leukemia virus (moloney strain) were studied. vinyl polymers themselves cannot act as templates for the polymerase. however, if a vinyl polymer is added to a polymerase reaction mixture in which a complementary polynucleotide serves as the template, the reaction is inhibited: thus with polyribocytidylic acid as template and oligodeoxyguanylic acid as primer, ne ...197656995
photic and pentylenetetrazol-induced seizure susceptibility in macaca mulatta.ninety-five out of 100 male macaca mulatta of varying ages from a restricted habitat in n. india showed no abnormal seizure susceptibility or eeg response to photic stimulation and no electro-clinical features of epilepsy after pentylenetetrazol, 40 mg/kg sc.c. autopsy studies on the remaining five monkeys with increased seizure proneness revealed depressed skull fractures with cortical trauma in four and a neoplasm in one. presumably, these factors were responsible for the lowered seizure susce ...197796259
purification of a chlamydia trachomatis-specific antigen by immunoadsorption with monospecific antibody.this study describes the isolation and partial characterization of a chlamydia trachomatis specific antigen. a species-specific antigen of c. trachomatis (antigen-0.65) was identified by two-dimensional immunoelectrophoresis. antiserum specific for antigen-0.65 was prepared in rabbits by immunizing with agarose-gel precipitates excised from two-dimensional immunoelectrophorograms. purified gamma-globulins from antigen-0.65 specific serum were coupled to the n-hydroxysuccinimide ester derivative ...197765424
colonic involvement in salmonellosis. 197765685
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