Publications

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[studies on biological properties of rickettsia mooseri following prolonged cultivation in the organism of the body louse. ii. oxidizing capacity of rickettsia mooseri in the presence of glutamic acid]. 195713531804
partial purification of rickettsia mooseri with a cation exchange resin. 195813562988
[biological properties of rickettsia mooseri following prolonged cultivation in the intestine of body louse. iii. electron microscopy of morphological aspects of rickettsia mooseri and prowazekii]. 195813582156
studies of rickettsial toxins. iv. cardiovascular functional abnormalities induced by rickettsia mooseri toxin in the white rat. 195813588002
[primary pneumopathy with hemagglutination positive to rickettsia mooseri]. 195913629326
[primary lung disease with positive sero-agglutination test to rickettsia mooseri]. 195913665722
the incorporation of labeled methionine by typhus rickettsiae. 195913670912
[a sole corpuscular antigen for agglutination and complement fixation reactions. properties and preparation of corpuscular antigens from rickettsia prowazekii and mooseri cultivated on white mouse lungs]. 196013686181
[biological properties of rickettsia mooseri after a prolonged cultivation in the intestine of body lice. iv]. 196013686182
the effects of paba derivatives on the multiplication of typhus rickettsiae. ii. mono- and bis-p-alkoxybenzoic acids. 196013708433
the effects of x-rays and beta rays (tritium) on the growth of rickettsia mooseri and rickettsia akari in embrvonate eggs.the growth of rickettsia mooseri was accelerated and quantitatively increased in embryonate eggs containing tritium oxide at levels of 180, 90, and 45 mc./egg during the growth period. the eggs of a group containing 22.5 mc./egg showed only a slight increase in the rate of growth of organisms; the infections in the eggs of a group given 11.2 mc./egg did not differ significantly from those of the control group. on the other hand, growth of r. akari was inhibited in embryonate eggs containing trit ...196013708436
metabolic studies of rickettsiae. v. metabolism of glutamine and asparagine in rickettsia mooseri. 196013710563
studies on rickettsial toxins. v. dissimilarity between the action of rickettsial mouse lethal toxin and bacterial endotoxin in mice. 196113786026
[effect of hyaluronidase on the course of rickettsia typhi infections in laboratory animals]. 196013786214
[complement fixing antibody against typhus group rickettsiae in sera of healthy korean]. 196213879394
cell-wall constituents of rickettsiae and psittacosis-lymphogranuloma organisms. 196313942448
properties of quinoxaline oxide-resistant rickettsia typhi.weiss, emilio (naval medical research institute, bethesda, md.) and harry r. dressler. properties of quinoxaline oxide-resistant rickettsia typhi. j. bacteriol. 83:415-417. 1962.-an effort was made to apply to the wilmington strain of rickettsia typhi the observation that in certain strains of rickettsiae and viruses of the psittacosis group quinoxaline oxide resistance is easily obtained and is often accompanied by other changes. resistance to quinoxaline oxide was obtained in r. typhi after 14 ...196214005861
[hemolytic effect of rickettsia prowazekii and rickettsia mooseri on erythrocytes from different species]. 196314062755
improvements in preparation of rickettsial type-specific and group-specific antigens. 196414106022
effect of dichlorotetrafluoroethane on the infectivity of viruses and rickettsiae sensitive to trichlorotrifluoroethane. 196314131240
[cultivation of some pathogenic rickettsia on noncellular polysynthetic nutrient media]. 196414187959
[thermal inactivation of toxic and hemolytic properties of rickettsia typhi]. 196414198037
[antigenic and immunogenic fractions of "whole" antigens from rickettsia cultured in eggs]. 196414198890
[intrapulmonary immunization against rickettsia mooseri and comparative evaluation of its effectiveness]. 196414198914
interaction of rickettsiae and phagocytic host cells. iv. early cellular response of man to typhus rickettsiae as revealed by the skin window technique, with observations on in vivo phagocytosis. 196414239942
[rickettsia typhis isolated in a wild rabbit]. 195514390805
studies on metabolism of rickettsiae. i. studies on dehydrogenases of rickettsia mooseri. 195914410573
[effect of cortisone on the course of rickettsia typhi infection in experimental animals. ii. effect of cortisone on the course of murine typhus in white rats and white mice]. 195914446097
studies on metabolism of rickettsiae. ii. incorporation of 14c amino acid mixture and 35s methionine by rickettsia mooseri. 196114457857
[note on the preservation of the murine typhus virus]. 196114480513
serologically detected "new" tick-borne zoonoses in eastern croatia.to establish serologically a contact with causative agents of human monocytic and granulocytic ehrlichiosis, human babesiosis, recently detected rickettsioses, and lyme disease in individuals with a history of tick bite from three counties in eastern croatia.200314515426
gene sequence-based criteria for identification of new rickettsia isolates and description of rickettsia heilongjiangensis sp. nov.we propose genetic guidelines for the classification of rickettsial isolates at the genus, group, and species levels by using sequences of the 16s rrna (rrs) gene and four protein-coding genes, the glta, ompa, and ompb genes and gene d. to be classified as a member of the genus rickettsia, an isolate should exhibit degrees of rrs and glta homology with any of the 20 rickettsia species studied of >/=98.1 and >/=86.5%, respectively. a member of the typhus group should fulfill at least two of the f ...200314662925
murine typhus--hawaii, 2002.murine typhus, a zoonotic disease caused by rickettsia typhi, is uncommon in the united states. hawaii typically reports five or six cases annually; however, 47 cases were reported in 2002. this report summarizes clinical data for three of these cases and describes murine typhus activity in hawaii during 2002 and control efforts of the hawaii department of health (hdh). the high number of reported cases in 2002 underscores the need for community education to prevent murine typhus and an assessme ...200314681594
facial paralysis and meningitis caused by rickettsia typhi infection.involvement of cranial nerves was rarely reported in various rickettsioses. we present here for the first time a case of peripheral facial paralysis associated with rickettsia typhi infection.200314723370
a sero-epidemiological study of rickettsia typhi infection in dogs from soria province, central spain.data relating to rickettsia typhi infection in spain are scarce. the seroprevalence of canine infection with this species has now been investigated, in dogs from the central province of soria. the results of indirect immunofluorescence assays indicated that nine (12.3%) of the 73 dogs checked were carrying antibodies against r. typhi. the gender, age and breed of the dog, and whether it was used for hunting, shepherding, guarding or simply as a pet, apparently had no significant affect on the pr ...200314754499
[strain of rickettsia mooseri of eastern sicily cultivated in vitelline sac of chick embryo]. 195014777276
[lysis of rickettsia after introduction into lice]. 195114905633
metabolic studies of rickettsiae. ii. studies on the pathway of glutamate oxidation by purified suspensions of rickettsia mooseri. 195214938541
studies on the toxicity of typhus rickettsiae. i. susceptibility of the white rat, with a note on pathologic changes. 195214946428
comparison of the effectiveness of five different antibiotic regimens on infection with rickettsia typhi: therapeutic data from 87 cases.this study estimated the clinical effectiveness of five different antibiotic regimens (doxycycline, chloramphenicol, ciprofloxacin, doxycycline plus chloramphenicol, and doxycycline plus ciprofloxacin) administered for infection with rickettsia typhi in terms of the duration of the fever. eighty-seven patients with endemic typhus were hospitalized between 1993 and 1998 at the general hospital of chania in chania, crete, greece. the mean time to defervescence was 2.9 days for doxycycline, 4.0 day ...200415155995
murine typhus with renal involvement in canary islands, spain.murine typhus and "murine-typhus-like" disease are reemerging infectious diseases. in canary islands (spain), a rather distinct clinical pattern characterized by higher incidence of complications, especially renal damage (including acute failure and urinalysis abnormalities), is apparent and highly suggestive. it could be related to different strains of rickettsia typhi or other cross-reactive species.200415200874
the etiology of febrile illness in adults presenting to patan hospital in kathmandu, nepal.in nepal, many infections remain poorly characterized, partly due to limited diagnostic facilities. we studied consecutive febrile adults presenting to a general hospital in kathmandu, nepal. of the 876 patients enrolled, enteric fever and pneumonia were the most common clinical diagnoses. putative pathogens were identified in 323 (37%) patients, the most common being salmonella enterica serotype typhi and s. enterica serotype paratyphi a (117), rickettsia typhi (97), streptococcus pneumoniae (5 ...200415211012
reemerging murine typhus, japan. 200415216852
[the history of the flea in art and literature].the flea has been, indirectly, one of the protagonists in the history of man. as one of the two vectors of yersinia pestis, the etiological agents of the black death, the flea (xenopsylla cheopis) has contributed, over the centuries, to the death of millions of people in many countries. galileo galilei was the first to observe the flea with a microscope (1624), but the credit of depicting it with a stunning drawing goes to the britisher robert hooke in 1665. a number of zoologists, including ant ...200415305680
complete genome sequence of rickettsia typhi and comparison with sequences of other rickettsiae.rickettsia typhi, the causative agent of murine typhus, is an obligate intracellular bacterium with a life cycle involving both vertebrate and invertebrate hosts. here we present the complete genome sequence of r. typhi (1,111,496 bp) and compare it to the two published rickettsial genome sequences: r. prowazekii and r. conorii. we identified 877 genes in r. typhi encoding 3 rrnas, 33 trnas, 3 noncoding rnas, and 838 proteins, 3 of which are frameshifts. in addition, we discovered more than 40 p ...200415317790
t cells mediate cross-protective immunity between spotted fever group rickettsiae and typhus group rickettsiae.rickettsioses are severe infections caused by obligately intracellular bacteria that preferentially infect the endothelium lining the vasculature. the causative agents, rickettsiae, have been divided according to biological, genetic, and antigenic parameters into 2 main groups: spotted fever and typhus. they have not been thought to stimulate cross-reactive protective immune responses; however, in this study, we show that, in relevant animal models that mimic human rickettsial infections, there ...200415346331
[not available]. 194915393373
studies on the antigenic substance common to proteus ox10, rickettsia prowazeki and rickettsia mooseri. 194915396952
[murine typhus in rio de janeiro. iii. culture and properties of strains of rickettsia mooseri isolated from naturally infected rats]. 195015412017
the effect of certain chemicals on rickettsia typhi infections in chick embryos. 195015415611
rickettsial vaccines: the old and the new.in the past century vaccine development for prevention of rickettsial diseases has been prolific. however, in the past 20 years no new rickettsial vaccine has been manufactured and there are currently no new or old rickettsial vaccines licensed. early rickettsial vaccines were difficult, expensive and very hazardous to produce. molecular biology techniques of today are currently being used to develop new rickettsial vaccines that are standardized, inexpensive, nonhazardous and efficacious.200415485334
rickettsioses and the international traveler.the rickettsioses--zoonotic bacterial infections transmitted to humans by arthropods--were for many years considered to be oddities in travel medicine. during the previous 2 decades, however, reports of >450 travel-associated cases have been published worldwide, the vast majority being murine typhus caused by rickettsia typhi, mediterranean spotted fever caused by rickettsia conorii, african tick bite fever caused by rickettsia africae, and scrub typhus caused by orientia tsutsugamushi. most pat ...200415546086
the bartonella henselae sucb gene encodes a dihydrolipoamide succinyltransferase protein reactive with sera from patients with cat-scratch disease.bartonella henselae is a recently recognized pathogenic bacterium associated with cat-scratch disease, bacillary angiomatosis and bacillary peliosis. a recombinant clone expressing an immunoreactive antigen of b. henselae was isolated by screening a genomic dna cosmid library by western blotting with sera pooled from patients positive for b. henselae igg antibodies by indirect immunofluorescence (ifa). the deduced amino acid sequence of the 43.7 kda encoded protein was found to be 76.3 % identic ...200415585501
a review of emerging flea-borne bacterial pathogens in new zealand.recent studies have shown that rickettsia typhi, r. felis, bartonella henselae, and b. clarridgeiae occur in new zealand. to raise awareness of these emerging and re-emerging flea-borne bacterial pathogens among new zealand health workers, we review the clinical features, diagnosis, and treatment of infections, and the biology of the major flea vectors.200515682209
functional analysis of seca homologues from rickettsiae.the molecular basis of protein secretion that underlines rickettsial pathogenesis remains unknown. this paper reports the molecular and functional analysis of the putative seca gene, an essential component of the sec-dependent protein secretion pathway, from rickettsia rickettsii and rickettsia typhi, the aetiological agents of rocky mountain spotted fever and murine typhus, respectively. the sequence analysis of the cloned seca genes from r. rickettsii and r. typhi show orfs of 2721 and 2718 nt ...200515699207
epidemiological study of rickettsia typhi infection in two provinces of the north of spain: analysis of sera from the general population and sheep.data relating to rickettsia typhi infection in spain are scarce. a serological survey of 383 serum samples (184 males, 199 females) from the general population and 120 sera from sheep were studied by immunofluorescence assay (ifa). the human serum samples were collected from the general population of palencia and burgos provinces, and sheep serum samples were collected from palencia province. of the 383 human serum samples studied, 29 were positive for antibodies against r. typhi (7.5%) no stati ...200516011432
clinical and laboratory features of murine typhus in central tunisia: a report of seven cases.murine or endemic typhus, caused by rickettsia typhi, has been reported in all continents. in the 1970s, no cases of murine typhus were diagnosed in tunisia.200516054415
rickettsial metk-encoded methionine adenosyltransferase expression in an escherichia coli metk deletion strain.the obligate intracellular bacterium rickettsia prowazekii has recently been shown to transport the essential metabolite s-adenosylmethionine (sam). the existence of such a transporter would suggest that the metk gene, coding for the enzyme that synthesizes sam, is unnecessary for rickettsial growth. genome sequencing has revealed that this is the case for the metk genes of the spotted fever group and the madrid e strain of r. prowazekii, which contain recognizable inactivating mutations. howeve ...200516077118
rickettsia felis infection rates in an east texas population.murine typhus (rickettsia typhi), endemic in southern california and south texas, is maintained within a host-vector system consisting of the opossum and cat flea. in the early 1990s, a second rickettsial species, rickettsia felis, was also found to be maintained within the opossum-cat flea system and is, in fact, found more commonly than r. typhi in the opossum and cat flea. recognized as a human pathogen in 1994, r. felis causes an infection that produces symptoms indistinguishable from classi ...200516130886
detection of medically important ehrlichia by quantitative multicolor taqman real-time polymerase chain reaction of the dsb gene.ehrlichia species are the etiological agents of emerging and life-threatening tick-borne human zoonoses, in addition to causing serious and fatal infections in companion animals and livestock. we developed the first tricolor taqman real-time polymerase chain reaction assay capable of simultaneously detecting and discriminating medically important ehrlichiae in a single reaction. analytical sensitivity of 50 copies per reaction was attained with templates from ehrlichia chaffeensis, ehrlichia ewi ...200516237220
serological evidence of infection with rickettsia typhi and rickettsia felis among the human population of catalonia, in the northeast of spain.murine typhus (mt) is a cause of fever of intermediate duration in the south of spain. rickettsia typhi has been described as the mt etiological agent. rickettsia felis produces an infection similar to mt. the aim of the study is to determine their seroprevalence in humans in catalonia. antibodies to rickettsia typhi and rickettsia felis from 217 serum samples were examined by indirect immunofluorescence assay (ifa). age, gender, residence area, contact with animals, and occupation were surveyed ...200616407356
antibodies to rickettsia rickettsii, rickettsia typhi, coxiella burnetii, bartonella henselae, bartonella quintana, and ehrlichia chaffeensis among healthy population in minas gerais, brazil.rickettsial diseases except those belonging to spotted fever group rickettsioses are poorly studied in south america particularly in brazil where few epidemiological reports have been published. we describe a serosurvey for rickettsia rickettsii, r. typhi, coxiella burnetii, bartonella henselae, b. quintana, and ehrlichia chaffeensis in 437 healthy people from a brazilian rural community. the serum samples were tested by indirected micro-immunoflourescence technique and a cutoff titer of 1:64 wa ...200616444416
characterisation of rickettsial diseases in a hospital-based population in malta.the aim of the study was to characterise the causative agents of rickettsial disease in malta. a secondary objective was to study the epidemiology of cases of rickettsial disease.200616473410
progress in rickettsial genome analysis from pioneering of rickettsia prowazekii to the recent rickettsia typhi.three rickettsial genomes have been sequenced and annotated. rickettsia prowazekii and r. typhi have similar gene order and content. the few differences between r. prowazekii and r. typhi include a 12-kb insertion in r. prowazekii, a large inversion close to the origin of replication in r. typhi, and loss of the complete cytochrome c oxidase system by r. typhi. r. prowazekii, r. typhi, and r. conorii have 13, 24, and 560 unique genes, respectively, and share 775 genes, most likely their essentia ...200516481486
growth of typhus group and spotted fever group rickettsiae in insect cells.to analyze the host dependency of rickettsial growth, nias-aeal-2 insect cells (aeal2) derived from mosquito were first used in this study. it was demonstrated that typhus group rickettsiae (tgr) grew well in aeal2 cells, but spotted fever group rickettsiae (sfgr) failed. to elucidate the inhibitory process of the growth of sfgr in aeal2 cells, the adherence and invasion were first analyzed. sfgr possessed abilities to adhere to and invade aeal2 cells as well as tgr in contrast to their inabilit ...200516481517
structural features of lipopolysaccharide from rickettsia typhi: the causative agent of endemic typhus.rickettsia typhi causes endemic typhus, a relatively mild, acute febrile illness characterized by headache and macular rash. it is maintained in rodents and transmitted to humans by flea xenopsylla cheopis. r. typhi contains a lipopolysaccharide thought to display a noticeable antigenic activity. we examined its structural features and it appears that the o-specific chain of the r. typhi lps is composed mainly of the alternating glc and quinac residues linked by 1-->4 bonds.200516481524
detection of a typhus group rickettsia in amblyomma ticks in the state of nuevo leon, mexico.the state of nuevo leon, mexico has had outbreaks of typhus group rickettsiosis, most recently recognized in 1997. evaluation of the sera of 345 patients with a dengue-like illness revealed that 25.5% had antibodies reactive with typhus group rickettsiae and 16% had antibodies to rickettsia parkeri. rickettsiae were detected by pcr and shell-vial isolations in the field-collected amblyomma ticks. molecular characterization by dna sequence analysis of the glta, ompb, and 17-kda gene identified th ...200516481535
rickettsial infections and fever, vientiane, laos.rickettsial diseases have not been described previously from laos, but in a prospective study, acute rickettsial infection was identified as the cause of fever in 115 (27%) of 427 adults with negative blood cultures admitted to mahosot hospital in vientiane, laos. the organisms identified by serologic analysis were orientia tsutsugamushi (14.8%), rickettsia typhi (9.6%), and spotted fever group rickettsia (2.6% [8 r. helvetica, 1 r. felis, 1 r. conorii subsp. indica, and 1 rickettsia "at1"]). pa ...200616494751
bartonella and rickettsia in fleas and lice from mammals in south carolina, u.s.a.species in the genera bartonella and rickettsia are vector-borne pathogens of humans and domestic animals. the natural reservoirs and enzootic transmission cycles of these bacteria are poorly known in south carolina. thirteen species of lice and fleas were collected from urban animals and screened for the presence of bartonella and rickettsia by pcr amplification using genus-specific primers. bartonella henselae was present in cat fleas (ctenocephalides felis) from virginia opossums (didelphis v ...200516599169
murine typhus mimicking acute cholecystitis in a traveller. 200616646753
louse-borne bacterial pathogens in lice (phthiraptera) of rodents and cattle from egypt.we collected 1,023 lice, representing 5 species, from rats and domestic cattle throughout 13 governorates in egypt and tested these lice for anaplasma marginale, bartonella spp., brucella spp., borrelia recurrentis, coxiella burnetii, francisella tularensis, and rickettsia spp. by pcr amplification and sequencing. five different louse-borne bacterial agents were detected in lice from rodents or cattle, including "bartonella rattimassiliensis", "b. phoceensis", and bartonella sp. near bartonella ...200616729688
seroepidemiological study of rickettsia felis, rickettsia typhi, and rickettsia conorii infection among the population of southern spain.rickettsia typhi and rickettsia conorii, the etiologic agents of, respectively, murine typhus and mediterranean spotted fever, are recognized as frequent causes of fever of intermediate duration in southern spain; in addition, in recent years rickettsia felis has been detected in potential vectors in this area. nevertheless, limited data exist regarding the actual prevalence of past infection due to these three pathogens. in the present study, the prevalence of past infection due to r. felis, r. ...200616767485
[murine typhus imported from senegal in a travelling immigrant]. 200616792948
widened coronary arteries in a feverish child.a 3-year-old girl with fever of unknown origin after a visit to surinam was seen at our hospital. signs and symptoms were indicative of either kawasaki syndrome or an acute viral or (atypical) bacterial illness. no cardiac abnormalities were noted at echocardiography. she was treated with intravenous immunoglobulin and clarithromycin and made a quick recovery. serologically, the diagnosis of murine typhus was made; a flea-borne rickettsiosis caused by rickettsia typhi. a follow-up echocardiograp ...200616830076
surveillance of egyptian fleas for agents of public health significance: anaplasma, bartonella, coxiella, ehrlichia, rickettsia, and yersinia pestis.serologic surveys in egypt have documented human and animal exposure to vector-borne bacterial pathogens, but the presence and distribution of these agents in arthropods has not been determined. between july 2002 and july 2003, fleas were collected from 221 mammals trapped in 17 cities throughout egypt. a total of 987 fleas were collected, representing four species (ctenocephalides felis, echidnophaga gallinacea, leptopsylla segnis, and xenopsylla cheopis); 899 of these fleas were x. cheopis fro ...200616837707
development of quantitative real-time pcr assays to detect rickettsia typhi and rickettsia felis, the causative agents of murine typhus and flea-borne spotted fever.rickettsia typhi and rickettsia felis are the etiologic agents of murine typhus and flea-borne spotted fever, respectively. we have constructed two quantitative real-time polymerase chain reaction (qpcr) assays to detect these pathogenic rickettsiae. the qpcr assays were developed utilizing unique sequences of the r. typhi and r. felis outer membrane protein b genes (ompb) to design the specific primers and molecular beacon probes. the assays were found to be species-specific and did not yield f ...200716893625
rickettsia felis in xenopsylla cheopis, java, indonesia.rickettsia typhi and r. felis, etiologic agents of murine typhus and fleaborne spotted fever, respectively, were detected in oriental rat fleas (xenopsylla cheopis) collected from rodents and shrews in java, indonesia. we describe the first evidence of r. felis in indonesia and naturally occurring r. felis in oriental rat fleas.200616965716
serologic evidence for rickettsia typhi and an ehrlichial agent in norway rats from baltimore, maryland, usa.we screened serum from 90 norway rats trapped in east baltimore, maryland, usa, from april to november 2005 for antibodies against rickettsia typhi and ehrlichia chaffeensis. six rats had positive titers of > or = 1:64 against r. typhi and did not react with r. akari. in addition, four rats had cross-reactive antibodies with titers of > or = 1:64 against ehrlichia chaffeensis. sera from these rats also cross-reacted with anaplasma phagocytophilum or ehrlichia muris. our data indicate that the ag ...200616989563
childhood encephalitis in crete, greece.this study included all 18 cases of children hospitalized for encephalitis in the referral university hospital of heraklion, crete, greece, during the 5-year period from 2000 to 2004. encephalitis was attributed to viral infection (echovirus, herpes simplex virus 1, varicella-zoster virus, cytomegalovirus, and influenza a) in eight children and to bacteria (mycoplasma pneumoniae, group a beta-hemolytic streptococcus, and rickettsia typhi) in a further five cases. multiple hyperintense brain lesi ...200617005114
molecular detection of rickettsia felis, rickettsia typhi and two genotypes closely related to bartonella elizabethae.a total of 56 fleas were collected from mice, rats, and one hedgehog in national parks of mainland portugal and the madeira island. all fleas were tested for the presence of bacteria of the genera rickettsia and bartonella using pcr assays. in fleas from mainland portugal, we detected rickettsia felis in one archaeopsylla erinacei maura flea and in one ctenophtalmus sp. in five leptopsylla segnis fleas taken from rats in the madeira island, we identified rickettsia typhi. in addition, in four fl ...200617038702
murine typhus from vietnam, imported into japan. 200617073110
the lspa gene, encoding the type ii signal peptidase of rickettsia typhi: transcriptional and functional analysis.lipoprotein processing by the type ii signal peptidase (spase ii) is known to be critical for intracellular growth and virulence for many bacteria, but its role in rickettsiae is unknown. here, we describe the analysis of lspa, encoding a putative spase ii, an essential component of lipoprotein processing in gram-negative bacteria, from rickettsia typhi. alignment of deduced amino acid sequences shows the presence of highly conserved residues and domains that are essential for spase ii activity ...200717098907
rickettsioses in australia.australia, an island continent in the southern hemisphere, has a range of rickettsial diseases that include typhus group rickettsiae (rickettsia typhi), spotted fever group rickettsiae (r. australis, r. honei), scrub typhus group rickettsiae (r. tsutsugamushi), and q fever (c. burnetii). our knowledge of australian rickettsiae is expanding with the recognition of an expanded range of r. honei (flinders island spotted fever) to tasmania and southeastern mainland australia (not just on flinders is ...200617114682
evidence of infection in humans with rickettsia typhi and rickettsia felis in catalonia in the northeast of spain.murine typhus is a cause of fever of intermediate duration in the south of spain, where antibodies against rickettsia typhi and rickettsia felis were observed in humans. this study presents the first report from the northeast of spain. human serum samples were tested by serological test. r. typhi and r. felis seroprevalences were 8.8% and 3.2%, respectively.200617114700
population survey of egyptian arthropods for rickettsial agents.between june 2002 and july 2003, 987 fleas, representing four species, and 1019 ticks, representing one argasid and eight ixodid species, were collected from egyptian animals. these arthropods were tested for rickettsial agents using polymerase chain reaction. dnas from anaplasma and ehrlichia spp. were detected in 13 ticks. previously undescribed bartonella spp. were detected in 21 fleas. coxiella burnetii was detected in two fleas and 20 ticks. rickettsia typhi was detected in 27 fleas from 10 ...200617114742
monitoring of humans and animals for the presence of various rickettsiae and coxiella burnetii by serological methods.serological examination of humans in slovakia suspected of having rickettsial infections revealed the presence of antibodies to spotted fever group rickettsiae (r. conorii, r. slovaca, and r. typhi). of interest is the finding of serological positivity to the newly recognized "irs" agent. antibodies to these rickettsiae and to c. burnetii were demonstrated also in domestic and hunting dogs and pet animals. these results confirm the occurrence and possible circulation of these rickettsiae and c. ...200617114784
early diagnosis of rickettsioses by electrochemiluminscence.the diagnosis of acute rickettsioses during the acute phase of the disease is challenging. we present preliminary evidence that antigen-capture using streptavidin-coated magnetic beads with biotinylated anti-rickettsia typhi rabbit polyclonal antibodies followed by electrochemiluminescent detection with ruthenylated antibodies of the same specificity could be used for the diagnosis of rickettsial diseases in the acute phase.200617114785
[meningitis revealing rickettsia typhi infection].neurological manifestations are rarely observed in murine typhus. we present a case of meningitis caused by rickettsia typhi.200717166631
a survey of zoonotic pathogens carried by norway rats in baltimore, maryland, usa.norway rats (rattus norvegicus) carry several zoonotic pathogens and because rats and humans live in close proximity in urban environments, there exists potential for transmission. to identify zoonotic agents carried by rats in baltimore, maryland, usa, we live-trapped 201 rats during 2005-2006 and screened them for a panel of viruses, bacteria, and parasites. antibodies against seoul virus (57.7%), hepatitis e virus (hev, 73.5%), leptospira interrogans (65.3%), bartonella elizabethae (34.1%), a ...200717224086
[murine typhus - rare cause of fever return from egypt].authors present a case report of 42-year male who spent 14 days in august of 2003 in egyptian seaside summer town hurghada and later he was admitted to the infectious disease department prague with the fever lasting 4 days. his symptoms were as follows: strong headache and dry cough. on the 7th and 8th day appeared transient maculopapular rash, laboratory test revealed a slightly elevated c reactive protein and elevation of amino transferases up to 5 times higher than range values, chest x-ray s ...200617230380
[fever of intermediate duration after return from the canary islands].a 35 year old patient presented to the emergency room with high fever, headache and a maculopapular rash after returning from the canary islands. elevated levels of ldh and transaminases and thrombopenia developed during the further hospital course. this presentation is common for an infection with rickettsia typhi. therapy with doxycycline is usually effective and should be instituted promptly. the patient's fever remitted 48 h after the first dose. fever of intermediate duration has been descr ...200717308911
serological and blood culture investigations of nepalese fever patients.serological testing of paired (i.e. admission and convalescent) sera from 103 fever patients in kathmandu, nepal, was performed to estimate the prevalence rates of scrub typhus, murine typhus, leptospira and dengue virus antibodies and to determine their role in the cause of active infections. blood cultures from 15 patients grew salmonella enterica serovar typhi, 8 grew s. paratyphi a and 6 grew other bacteria. diagnostic antibody levels were detected against murine typhus (27/103; 26%), scrub ...200717433390
murine typhus in children, south texas.children from south texas were evaluated for immunoglobulin g to rickettsia typhi, the causative agent of murine typhus. of 513 children, 8.6% of those 1-5 years of age, 13.3% of those 6-11 years of age, and 13.8% of those 12-17 years of age had positive results.200717553239
murine typhus in cyprus: 21 paediatric cases.the aim of this article is to present the manifestations of rickettsia typhi infection in childhood. twenty-one children under 15 years of age were hospitalised in the department of paediatrics of the archbishop makarios hospital in nicosia, cyprus, from 2000 to 2006 with rickettsia typhi infection. ten of them were boys and 11 were girls. the median age was eight years (range four to 13 years). the most common clinical features were fever (100%) and rash (57%). lymphadenopathy, usually cervical ...200717554571
comparative analysis of host-cell signalling mechanisms activated in response to infection with rickettsia conorii and rickettsia typhi.the gram-negative intracellular bacteria rickettsia conorii and rickettsia typhi are the aetiological agents of mediterranean spotted fever and endemic typhus, respectively, in humans. infection of endothelial cells (ecs) lining vessel walls, and the resultant vascular inflammation and haemostatic alterations are salient pathogenetic features of both of these rickettsial diseases. an important consideration, however, is that dramatic differences in the intracellular motility and accumulation pat ...200717577053
murine typhus in pregnancy: a case report from cyprus.r. typhi is one of the rickettsial species endemic in the mediterranean countries and is associated with the zoonotic infection of murine typhus, which may have a complicated course especially in adult patients. murine typhus in pregnancy has rarely been reported in the medical literature. we describe a case of murine typhus in a pregnant woman who was effectively treated with erythromycin.200717577832
concurrent infections in acute febrile illness patients in egypt.we report the occurrence of concurrent infections with multiple acute febrile illness (afi) pathogens during an ongoing prospective laboratory-based surveillance in four infectious disease hospitals in urban and rural areas of egypt from june 2005 to august 2006. patients were screened for leptospira, rickettsia typhi, brucella, or salmonella enterica serogroup typhi by various methods including serology, culture, and pcr. one hundred eighty-seven of 1,510 patients (12.4%) evaluated had supporti ...200717690420
seroepidemiological survey of rodents collected at a u.s. military installation, yongsan garrison, seoul, republic of korea.a seroepidemiological study of selected rodent-borne diseases (hantavirus [seoul [seo] virus], scrub typhus [orientia tsutsugamushi], murine typhus [rickettsia typhi], and leptospirosis [leptospira interrogans]), as part of the u.s. military rodent surveillance and control program, was conducted from 2001 through 2005 at yongsan garrison, seoul, republic of korea. rodents were collected to determine the prevalence of rodent-borne diseases at a u.s. military installation in an urban environment. ...200717691691
sensorineural hearing loss complicating severe rickettsial diseases: report of two cases.sub-acute focal neurological manifestations are reported rarely in systemic rickettsial diseases and are considered to be secondary to immune-mediated mechanisms. we present two cases of transient sensorineural hearing loss complicating the course of severe rickettsial diseases caused by rickettsia typhi and rickettsia conorii, respectively. the diagnosis was based on the presence of high igm antibodies and the prompt response to doxycycline treatment. in both cases, hearing loss presented durin ...200818023483
real-time multiplex pcr assay for detection and differentiation of rickettsiae and orientiae.the high incidence of rickettsial diseases in southeast asia necessitates rapid and accurate diagnostic tools for a broad range of rickettsial agents, including orientia tsutsugamushi (scrub typhus) and rickettsia typhi (murine typhus), but also spotted fever group infections, which are increasingly reported. we present an sybr-green-based, real-time multiplex pcr assay for rapid identification and differentiation of scrub typhus group, typhus group and spotted fever group rickettsiae using 47kd ...200818093627
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