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[microsurgery in gynecology--indications, results and limitations]. 20133616963
observations on the use of anaplasma centrale for immunization of cattle against anaplasmosis in zimbabwe.a total of 93 bos taurus cattle was used in pen trials to compare vaccine stocks of anaplasma centrale from south africa and australia (which stock came from south africa in 1934) in protecting against three virulent field isolates from clinical anaplasma marginale infections. in addition, field observations were made on the use of a vaccine, prepared from the australian stock, in over 9553 cattle of mixed age and breeds on 16 co-operator farms and at one communal dip. the results of the pen tri ...19989741051
prevalence and genetic diversity of anaplasma marginale strains in cattle in south africa.bovine anaplasmosis, caused by the tick-borne rickettsia anaplasma marginale, is endemic in south africa and results in considerable economic loss to the cattle industry. this study was designed to characterize strains of a. marginale at the molecular level from cattle raised in communal and commercial farms in the north-eastern and south-western regions of the free state province, south africa, that varied in rainfall and vegetation. seroprevalence to a. marginale was determined in 755 cattle b ...200717359443
experimental transmission of field anaplasma marginale and the a. centrale vaccine strain by hyalomma excavatum, rhipicephalus sanguineus and rhipicephalus (boophilus) annulatus ticks.the cattle rickettsia anaplasma marginale is distributed worldwide and is transmitted by about 20 tick species, but only rhipicephalus simus, a strictly african tick species, has been shown to transmit the vaccine strain of a. centrale. the aim of the present study was to examine transmission of field strains of a. marginale and of the vaccine strain of a. centrale by three tick species -hyalomma excavatum, rhipicephalus sanguineus and rhipicephalus (boophilus) annulatus - to susceptible calves. ...200918823724
expression of major surface protein 2 variants with conserved t-cell epitopes in anaplasma centrale vaccinates.major surface protein 2 (msp-2), identified as a protection-inducing immunogen against anaplasma marginale challenge, is an immunodominant outer membrane protein with orthologues in all examined anaplasma species. although immunization with live anaplasma centrale has long been used to induce protection against acute disease upon challenge with virulent a. marginale, its msp-2 structure and whether msp-2 variants are generated during persistence of the vaccine strain was unknown. in this study, ...200211796593
humoral immune response and hematologic evaluation of pregnant jersey cows after vaccination with anaplasma centrale.the main objective of this work was to evaluate the safety of an anaplasma centrale vaccine in pregnant pure bred jersey cows selected from a herd located at miranda state, venezuela. ten cows of 3-5 months of gestation were chosen and previous vaccination all cows were tested for anaplasma antibodies by the indirect immunofluorescence assay (ifa), so only seronegative cows were included in the group, and for blood parameters, rectal temperature, and pregnancy. selected cows were vaccinated intr ...200312829387
synthesis and migration of maize rough dwarf virus in the host cell: an autoradiographic study. 20154427107
[review notes on toxoplasmosis]. 20164987998
supraclavicular catheterization of the superior vena cava. 20164836144
[total invagination of the appendix, simulating a cecal neoplasm]. 20164714895
overcoming constraints to meeting increased demand for babesia bigemina vaccine in australia.demand for live trivalent tick fever vaccine containing babesia bovis, babesia bigemina and anaplasma centrale produced by the department of primary industries, queensland, has increased from less than 10,000 doses in 1988 to 500,000 doses in 2001. this paper describes a series of trials aimed at overcoming certain constraints to obtain b. bigemina parasitised erythrocytes (pes) on a large enough scale from infected splenectomised calves to meet the demand. passage through a series of splenectom ...200312935736
independence of anaplasma marginale strains with high and low transmission efficiencies in the tick vector following simultaneous acquisition by feeding on a superinfected mammalian reservoir host.strain superinfection occurs when a second pathogen strain infects a host already carrying a primary strain. anaplasma marginale superinfection occurs when the second strain carries a variant repertoire different from that of the primary strain, and the epidemiologic consequences depend on the relative efficiencies of tick-borne transmission of the two strains. following strain superinfection in the reservoir host, we tested whether the presence of two a. marginale (sensu lato) strains that diff ...200919188360
identification of anaplasma centrale major surface protein-2 pseudogenes.the present study was aimed to identify msp2 pseudogenes and msp2 variants in the vaccine anaplama centrale strain. five msp2 pseudogenes were identified in the a. centrale genome, and multiple msp2 variants that emerged during both acute and persistent infection were detected. the pseudogene copies of msp2 were truncated; they contained a central hypervariable region flanked by short portions of the 5' and 3' conserved regions. alignment of the hypervariable region sequence of the expression si ...201020036077
[continuing education in pediatrics in the 1980's]. 20154825280
comparative study of a hypnotic and a tranquillizer in anxiety. 20164893720
[evaluation of adaptation to physical loads in patients with hypertension]. 20144094298
stapedectomy or a hearing aid for otosclerosis? 20164766684
multiple pathogens including potential new species in tick vectors in côte d'ivoire.our study aimed to assess the presence of different pathogens in ticks collected in two regions in côte d'ivoire.201626771308
theileria parva genetic diversity and haemoparasite prevalence in cattle and wildlife in and around lake mburo national park in uganda.wildlife, especially cape buffalo (syncerus caffer), are thought to act as a reservoir for many of the important tick-borne pathogens of cattle. in this study, we have determined the prevalence of the most significant tick-borne haemoparasites in wildlife (buffalo, impala, eland and bushbuck) as well as in cattle grazing inside and neighbouring lake mburo national park (lmnp) in uganda. a high percentage of buffalo were carriers of theileria parva, theileria mutans, theileria velifera, theileria ...201120827491
[angiotomography of supratentorial brain tumors]. 2003207124
detection of anaplasma phagocytophilum from wild boars and deer in japan.this study was conducted to determine the prevalence and geographic distribution of anaplasma phagocytophilum, the causative agent of human granulocytic anaplasmosis, in wild deer and boars in japan. we analyzed the blood samples of the study animals using pcr-targeting the 16s rdna followed by dna sequencing. wild deer infected with anaplasma spp., including anaplasma bovis and anaplasma centrale were detected in the region from hokkaido to kyushu. the infection rates of a. phagocytophilum, a. ...201121788712
[histochemical detection of glycogen and rna in inflammatory hyperplasia of the palatal mucosa]. 20154516964
[transketolase activity as a parameter for the vitamin b1 provision in some dermatoses (herpes zoster, psoriasis, drug eruptions, excema, and urticaria) (author's transl)]. 2003153861
[perspectives in the drug therapy of peptic ulcer]. 20102897487
[irrigation solutions in urology]. 20133847666
[the control of renin secretion: experimental data and clinical implications]. 2004356832
[spectroscopic study of some substituted n-(2-diethylamino-ethyl) benzamides]. 20154774835
site of injection for vaccination. 20061392898
value of 1-alpha-hydroxy vitamin d3 in treatment of primary hyperparathyroidism before parathyroidectomy.the postoperative course of six patients with primary hyperparathyroidism and obvious radiological evidence of bone disease pretreated with 1-alpha-hydroxy vitamin d3 (1 alpha hcc) was indistinguishable from that of six patients with a similar clinical and radiological picture who were not pretreated. 1alphahcc may increase the hypercalcaemia in some cases and cannot be recommended for the routine preparation of such patients for surgery.2004427402
[shock (peripheral circulatory insufficiency). i]. 2002119989
plasma progesterone concentrations in female little brown bats (myotis lucifugus) during hibernation.monthly collections of hibernating little brown bats contained (1) nulliparous females with small uteri and no antral follicles, (2) nulliparous females with swollen uteri and mature follicles, and (3) parous females, which, despite obvious differences in reproductive status, had equivalent plasma progesterone values. during the principal study season, mean monthly progesterone concentrations (measured by radioimmunoassay) showed recurrent increases with an apparent periodicity of about 60 days, ...20123397958
[knee symptoms in children]. 20143974742
conversion of calciferols to isotachysterols by trifluoroacetic acid. 20154540598
[use of composition sk-1 for the gingivitis therapy of children and adolescents undergoing orthodontic treatment]. 20143866395
anaplasma marginale and theileria annulata in questing ticks from portugal.ticks are ubiquitous arthropods and vectors of several pathogenic agents in animals and humans. monitoring questing ticks is of great importance to ascertain the occurrence of pathogens and the potential vector species, offering an insight into the risk of disease transmission in a given area. in this study 428 host-seeking ticks, belonging to nine species of ixodidae and collected from 17 of the 23 portuguese mainland subregions, were screened for several tick-borne agents with veterinary relev ...201627394441
anaplasmataceae in wild ungulates and carnivores in northern spain.wild vertebrates are essential hosts for tick-borne diseases but data on the prevalence and diversity of anaplasma spp. in wildlife are scarce. in this study, we used real-time pcr to investigate the distribution of anaplasma species in spleen samples collected from 625 wild animals (137 cervids, 227 wild boar, and 261 carnivores) in two regions in northern spain. a first generic real-time pcr assay was used to screen for the presence of anaplasma spp. followed by a second species-specific multi ...201626596894
relationship between self-control and alcohol consumption patterns and problems of college students.the relationship between self-control strategies, alcohol-related problems and the quantity and frequency of alcohol use was examined. analyses of data from 410 university students who completed the student alcohol questionnaire and the self-control questionnaire indicated that both internal and external self-control strategies were significantly correlated with alcohol-related problems and alcohol consumption variables. self-control strategies were also found to be applied differentially across ...20123347074
panic disorder, vertigo, and the protirelin (trh) test. 20113133685
cross-protection between geographically distinct anaplasma marginale isolates appears to be constrained by limited antibody responses.the rickettsia anaplasma marginale causes the haemolytic disease bovine anaplasmosis, an economic problem in tropical and subtropical areas worldwide. the closely related but less pathogenic anaplasma centrale is commonly used as a live vaccine to prevent anaplasmosis, but it can only be produced from infected blood. ufmg1 is a low pathogenic brazilian strain of a. marginale, which has been shown to protect cattle against a high pathogenic brazilian isolate. as ufmg1 can be grown in tick cells, ...201324589108
international conference on herpetic eye diseases. north lake tahoe, california, usa, september 6-8, 1986. proceedings. 20133829697
selective differentiation between amyloid and connective tissue structures based on the collagen specific topo-optical staining reaction with congo red. 20144108771
viability assays of intra-erythrocytic organisms using fluorescent dyes.three intra-erythrocytic tick fever organisms of cattle (babesia bovis, babesia bigemina and anaplasma centrale) were subjected to a range of stressors, including heat, storage over time, specific chemotherapy and cryopreservation. various stains, both alone and in combination, were used in an attempt to assess viability of these organisms before and after the stressors were applied. carboxyfluorescein diacetate succinimidyl ester (cfse) stained live babesia spp. very well while fluorescein diac ...200919380205
sir arnold theiler and the discovery of anaplasmosis: a centennial perspective.sir arnold theiler's research in 1908/09 led to the discovery of the first rickettsial pathogen, anaplasma marginale, and set the stage for his development and implementation of an effective live vaccine based on a less virulent strain, a. marginale ss. centrale. his 1910 report, describing a. marginale, is among the classic monographs in infectious disease research, presenting not only observations in exacting detail but also highlighting the deductive reasoning leading to association of a new ...200919967931
identification of anaplasma marginale outer membrane protein antigens conserved between a. marginale sensu stricto strains and the live a. marginale subsp. centrale vaccine.live vaccination with anaplasma marginale subsp. centrale (synonym for anaplasma centrale) induces protection against severe disease upon challenge with a. marginale sensu stricto strains. despite over a century of field use, the targets of protective immunity remained unknown. using a broad proteomic approach, we identified the proteins in a challenge sensu stricto strain that were bound by the relevant antibody isotype induced by live vaccination with anaplasma marginale subsp. centrale. a cor ...201021189322
tick-borne haemoparasites in african buffalo (syncerus caffer) from two wildlife areas in northern botswana.the african buffalo (syncerus caffer) is a host for many pathogens known to cause economically important diseases and is often considered an important reservoir for livestock diseases. theileriosis, heartwater, babesiosis and anaplasmosis are considered the most important tick-borne diseases of livestock in sub-saharan africa, resulting in extensive economic losses to livestock farmers in endemic areas. information on the distribution of tick-borne diseases and ticks is scarce in northern botswa ...201525589064
retrospective study of hemoparasites in cattle in southern italy by reverse line blot hybridization.tick-borne diseases are widespread in tropical and temperate regions and are responsible for important economic losses in those areas. in order to assess the presence and prevalence of various pathogens in southern italy, we retrospectively analyzed cattle blood samples collected for a previous study in 2000 using reverse line blot (rlb) hybridization. the study had been carried out in three regions of southern italy on 1,500 randomly selected and apparently healthy adult cattle. rlb showed that ...201424614604
identification of multilocus genetic heterogeneity in anaplasma marginale subsp. centrale and its restriction following tick-borne transmission.anaplasma marginale subsp. centrale was the first vaccine used to protect against a rickettsial disease and is still in widespread use a century later. as its use preceded development of either cryopreservation or cell culture, the vaccine strain was maintained for decades by sequential passage among donor animals, excluding the natural tick-borne transmission cycle that provides a selective pressure or population "bottleneck." we demonstrated that the vaccine strain is genetically heterogeneous ...201323509140
molecular diagnosis and genetic diversity of tick-borne anaplasmataceae agents infecting the african buffalo syncerus caffer from marromeu reserve in mozambique.tick-borne diseases (tbds) are very important in relation to domestic ruminants, but their occurrence among wild ruminants, mainly in the african buffalo syncerus caffer, remains little known.201627531003
guidelines for the direct detection of anaplasma spp. in diagnosis and epidemiological studies.the genus anaplasma (rickettsiales: anaplasmataceae) comprises obligate intracellular gram-negative bacteria that are mainly transmitted by ticks, and currently includes six species: anaplasma bovis, anaplasma centrale, anaplasma marginale, anaplasma phagocytophilum, anaplasma platys, and anaplasma ovis. these have long been known as etiological agents of veterinary diseases that affect domestic and wild animals worldwide. a zoonotic role has been recognized for a. phagocytophilum, but other spe ...201728055579
molecular detection of tick-borne rickettsiales in goats and sheep from southeastern china.members from rickettsiales such as anaplasma phagocytophilum, ehrlichia chaffeensis, and some spotted fever group (sfg) rickettsiae are important tick-borne pathogens. one hundred goats and sheep from southeastern china were examined for the presence of anaplasma, e. chaffeensis, and sfg rickettsiae by pcr. a. phagocytophilum, anaplasma bovis, and anaplasma centrale were detected in 15, 49, and 16 samples, respectively. the a. phagocytophilum and a. centrale were highly homologous to strains fro ...201626872274
first molecular survey and novel genetic variants' identification of anaplasma marginale, a. centrale and a. bovis in cattle from tunisia.few data are available about the presence and distribution of anaplasma species in cattle in north african countries. in this study prevalence, co-infections, risk factors and genetic diversity of anaplasma species were evaluated in bovines from northern tunisia. a total of 232 cattle from 36 randomly selected farms in three tunisian localities were investigated for the presence of anaplasma species in blood by real-time pcr and/or nested pcr. overall infection rates of anaplasma spp., anaplasma ...201526117444
detection of tick-borne anaplasma bovis, anaplasma phagocytophilum and anaplasma centrale in spain.the genus anaplasma (rickettsiales: anaplasmataceae) includes species of medical and veterinary importance. the presence of anaplasma spp. in ticks from birds, as well as in haemaphysalis punctata (ixodida: ixodidae) specimens collected from cattle and vegetation in northern spain was investigated. a total of 336 ticks from birds [174 ixodes frontalis (ixodida: ixodidae), 108 h. punctata, 34 hyalomma marginatum (ixodida: ixodidae), 17 ixodes ricinus (ixodida: ixodidae) and three ixodes spp.], an ...201526111122
bovine anaplasmosis in turkey: first laboratory confirmed clinical cases caused by anaplasma phagocytophilum.anaplasma species are obligate intracellular rickettsial pathogens that affect the health of humans and other animals. clinical cases of anaplasmosis caused by anaplasma phagocytophilum were evaluated, and the frequency of bovine anaplasma species was determined in cattle. blood samples and thin blood smears were collected from 10 cattle exhibiting clinical signs of tick-borne fever. in addition, blood samples were collected from 123 apparently healthy cattle from the same area. dna was screened ...201526051478
development of a new pcr-based assay to detect anaplasmataceae and the first report of anaplasma phagocytophilum and anaplasma platys in cattle from algeria.bovine anaplasmosis is a hemoparasitic disease considered as a major constraint to cattle production in many countries. this pathology is at least partially caused by anaplasma phagocytophilum, anaplasma marginale, anaplasma centrale, and anaplasma bovis. the global threat and emergence of these species in animals require the reliable identification of these bacteria in animal samples. in this study, we developed a new qpcr tool targeting the 23s rrna gene for the detection of anaplasmataceae ba ...201525748051
co-circulation of multiple species of rickettsiales bacteria in one single species of hard ticks in shenyang, china.bacteria in the order rickettsiales include some of the most important zoonotic (re)emerging pathogens for animals and humans. in 2012, a total of 1267 adult haemaphysalis longicornis ticks were collected from domestic animals (cattle and sheep) in shenyang of liaoning province, china. these ticks were grouped into 181 pools (each pool with 6-7 ticks). rickettsiales agents were identified in 93 (51.38%) tick pools using pcr targeting rrs (16s rrna). in addition to 16s rrna gene sequences, glta a ...201425131152
comparison of three nucleic acid-based tests for detecting <i>anaplasma marginale</i> and <i>anaplasma centrale</i> in cattle.several nucleic acid-based assays have been developed for detecting anaplasma marginale and anaplasma centrale in vectors and hosts, making the choice of method to use in endemic areas difficult. we evaluated the ability of the reverse line blot (rlb) hybridisation assay, two nested polymerase chain reaction (npcr) assays and a duplex real-time quantitative polymerase chain reaction (qpcr) assay to detect a. marginale and a. centrale infections in cattle (n = 66) in south africa. the lowest dete ...201728155283
identification of a t-cell epitope that is globally conserved among outer membrane proteins (omps) omp7, omp8, and omp9 of anaplasma marginale strains and with omp7 from the a. marginale subsp. centrale vaccine strain.within the protective outer membrane (om) fraction of anaplasma marginale, several vaccine candidates have emerged, including a family of om proteins (omps) 7 to 9, which share sequence identity with each other and with the single protein omp7 in the vaccine strain a. marginale subsp. centrale. a. marginale omps 7 to 9 are logical vaccine candidates because they are surface exposed, present in the om immunogen and protective cross-linked om proteins, recognized by immune serum igg2 and t cells i ...201727795302
characterization of anaplasma marginale subsp. centrale strains by use of msp1as genotyping reveals a wildlife reservoir.bovine anaplasmosis caused by the intraerythrocytic rickettsial pathogen anaplasma marginale is endemic in south africa. anaplasma marginale subspecies centrale also infects cattle; however, it causes a milder form of anaplasmosis and is used as a live vaccine against a. marginale there has been less interest in the epidemiology of a. marginale subsp. centrale, and, as a result, there are few reports detecting natural infections of this organism. when detected in cattle, it is often assumed that ...201627440819
molecular survey and sequence analysis of anaplasma spp. in cattle and ticks in a malaysian farm.this study was conducted to determine the occurrence of anaplasma spp. in the blood samples of cattle, goats, deer and ticks in a malaysian farm. using polymerase chain reaction (pcr) and sequencing approach, anaplasma spp. was detected from 81(84.4%) of 96 cattle blood samples. all blood samples from 23 goats and 22 deer tested were negative. based on the analysis of the anaplasma partial 16s ribosomal rna gene, four sequence types (genotypes 1 to 4) were identified in this study. genotypes 1-3 ...201425776603
evaluation of the immune response to anaplasma marginale msp5 protein using a hsv-1 amplicon vector system or recombinant protein.anaplasma marginale is an intraerythrocytic vector-borne infectious agent of cattle. immunization with the current vaccine, based on parasitized erythrocytes with live anaplasma centrale, shows some constraints and confers partial protection, suggesting the feasibility for the development of new generation of vaccines. the aim of the present study was to assess the effect of sequential immunization of balb/c mice, with herpesvirus amplicon vector-based vaccines combined with protein-based vaccin ...201425458492
[prevalence of anaplasma spp. infection in a desert landscape region of heshuo, xinjiang].to study the prevalence of anaplasmosis among human, domestic sheep and tick population in heshuo area, southern xinjiang and to investigate the diversity of anaplasma species.201323751470
haemoparasite prevalence and theileria parva strain diversity in cape buffalo (syncerus caffer) in uganda.cape buffalo (syncerus caffer) are considered to be an important reservoir for various tick-borne haemoparasites of veterinary importance. in this study we have compared the haemoparasite carrier prevalence in buffalo from four geographically isolated national parks in uganda [lake mburo national park (lmnp), queen elizabeth national park (qenp), murchison falls national park (mfnp) and kidepo valley national park (kvnp)]. differences were seen in haemoparasite prevalence in buffalo from the fou ...201021074945
tick-borne pathogens of zoonotic and veterinary importance in nigerian cattle.ticks and tick-borne diseases undermine cattle fitness and productivity in the whole of sub-saharan africa, including nigeria. in this west african country, cattle are challenged by numerous tick species, especially during the wet season. consequently, several tbds are known to be endemic in nigerian cattle, including anaplasmosis, babesiosis, cowdriosis and theilerioris (by theileria mutans and theileria velifera). to date, all investigations on cattle tbds in nigeria have been based on cytolog ...201627090756
propagation of the israeli vaccine strain of anaplasma centrale in tick cell lines.anaplasma centrale has been used in cattle as a live blood vaccine against the more pathogenic anaplasma marginale for over 100 years. while a. marginale can be propagated in vitro in tick cell lines, facilitating studies on antigen production, immunisation and vector-pathogen interaction, to date there has been no in vitro culture system for a. centrale. in the present study, 25 cell lines derived from 13 ixodid tick species were inoculated with the israeli vaccine strain of a. centrale and mon ...201526210950
molecular detection and characterization of anaplasma species in wild deer and boars in gifu prefecture, japan. 201728250258
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