Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| field observations on the duration of immunity in cattle after vaccination against anaplasma and babesia species. | in an outbreak of babesia bovis in a large herd of friesian x malawi zebu cattle, which occurred after an interruption of intensive dipping, clinical or fatal babesiosis occurred in 54/299 (18.1%) animals which had never been vaccinated, as compared to 9/153 (5.9%) vaccinated animals. eight of the nine affected vaccinates had been vaccinated more than 27 months previously. sera were collected every 3-4 months from 33 friesian x malawi zebu heifers maintained with intensive dipping and vaccinated ... | 1996 | 8848296 |
| improving the specificity of indirect immunofluorescence for the serological diagnosis of bovine anaplasmosis. | fluorescing inclusion appendages were detected consistently in preparations of anaplasma centrale and anaplasma marginale when they were used as antigen in indirect immunofluorescence serological tests for the diagnosis of anaplasmosis in cattle. the presence of the inclusion appendages made it possible to confirm the specificity of the immunofluorescent reaction and to determine end-points with accuracy. | 1996 | 8848303 |
| comparison of the infectivity of babesia bovis, babesia bigemina and anaplasma centrale for cattle after cryopreservation in either dimethylsulphoxide (dmso) or polyvinylpyrrolidone (pvp). | 1997 | 9034504 | |
| babesial antibody dynamics after cattle immunisation with live vaccines, measured with an indirect immunofluorescence test. | the efficacy of vaccination of argentinean cattle against babesiosis and anaplasmosis using live immunogens was tested to detect specific antibodies in samples obtained about 60 days after vaccination. under these conditions a higher than expected proportion of cattle failed to show antibodies against babesia bigemina. therefore, a study was designed to evaluate if this failure was due to insensitivity of the routine test to detect antibodies to b. bigemina or to lack of infectivity of the live ... | 1997 | 9195707 |
| vaccines against hemoparasitic diseases in israel with special reference to quality assurance. | four vaccines against hemoparasitic diseases (anaplasmosis, babesiosis and theileriosis) and a vaccine against besnoitiosis are currently used in israel. these vaccines contain live attenuated parasites derived from cell culture (theileria annulata and besnoitia besnoiti) or from blood of infected, splenectomized calves (babesia bigemina, b. bovis and anaplasma centrale). cryopreserved master seed is used to initiate production of the vaccines. quality control performed during the preproduction ... | 1997 | 9512751 |
| observations on the use of anaplasma centrale for immunization of cattle against anaplasmosis in zimbabwe. | a total of 93 bos taurus cattle was used in pen trials to compare vaccine stocks of anaplasma centrale from south africa and australia (which stock came from south africa in 1934) in protecting against three virulent field isolates from clinical anaplasma marginale infections. in addition, field observations were made on the use of a vaccine, prepared from the australian stock, in over 9553 cattle of mixed age and breeds on 16 co-operator farms and at one communal dip. the results of the pen tri ... | 1998 | 9741051 |
| comparison of a competitive inhibition elisa and the card agglutination test for detection of antibodies to anaplasma marginale and anaplasma centrale in cattle. | to compare a recently developed recombinant msp-5 competitive inhibition elisa with a card agglutination test for detection of antibodies to anaplasma marginale and anaplasma centrale in australian cattle. | 1999 | 10330556 |
| evaluation of a commercially available elisa kit for detection of antibodies to anaplasma marginale and anaplasma centrale in cattle in australia and zimbabwe. | a newly available competitive inhibition elisa kit for the serological diagnosis of anaplasmosis was evaluated in australia and zimbabwe. in australia the performance of the test was compared with the card agglutination test (cat). the assay was evaluated using negative sera collected from anaplasma-free herds, positive sera from experimentally infected cattle and sera from anaplasma marginale-endemic herds. the sensitivity and specificity of the elisa in australia were 100 % and 83,3 %, respect ... | 2000 | 11028743 |
| anaplasmosis control and diagnosis in south africa. | anaplasmosis is widespread in south africa with more than 99% of the total cattle population at risk. five tick species have been experimentally shown to be capable of transmitting anaplasma in south africa. mechanical transmission through blood contaminated instruments and biting flies also occurs. vaccination against anaplasma marginale by administration of an anaplasma centrale live-blood vaccine has been practiced in this country since 1912. although generally a mild pathogen, anaplasma cent ... | 2000 | 11193662 |
| analysis of the 16s rrna gene sequence of anaplasma centrale and its phylogenetic relatedness to other ehrlichiae. | the nucleotide sequence of the anaplasma centrale 16s rrna gene was determined and compared with the sequences of ehrlichial bacteria. the sequence of a. centrale was closely related to anaplasma marginale by both level-of-similarity (98.08% identical) and distance analysis. a species-specific pcr was developed based upon the alignment data. the pcr can detect a. centrale dna extracted from 10 infected bovine red blood cells in a reaction mixture. a. centrale dna was amplified in the reaction, b ... | 2001 | 11238202 |
| identification of antigenic differences that discriminate between cattle vaccinated with anaplasma centrale and cattle naturally infected with anaplasma marginale. | monoclonal antibodies were raised against the vaccine strain of anaplasma centrale used in australia. a monoclonal antibody that reacted with an 80 kda antigen was used to develop an a. centrale-specific fluorescent antibody test that will be useful for confirming species identity in patent infections. another monoclonal antibody that reacted with a 116 kda antigen was used to develop an a. centrale-specific competitive inhibition enzyme-linked immunosorbent assay (elisa) for the serological ide ... | 2001 | 11239938 |
| use of repetitive dna elements to define genetic relationships among anaplasma marginale isolates. | anaplasma marginale genomic dna was tested for the presence of repetitive extragenic palindromic (rep) and enterobacterial repetitive intergenic consensus (eric)-like sequences in order to evaluate the genetic diversity of multiple a. marginale isolates. a. marginale isolates were obtained from cattle of six different states of brazil, from the us and an anaplasma centrale strain was obtained from uruguay. patterns obtained from a. marginale isolates varied from 14 to 17 fragments by rep-polymer ... | 2001 | 11313126 |
| citrate synthase gene sequence: a new tool for phylogenetic analysis and identification of ehrlichia. | the sequence of the citrate synthase gene (glta) of 13 ehrlichial species (ehrlichia chaffeensis, ehrlichia canis, ehrlichia muris, an ehrlichia species recently detected from ixodes ovatus, cowdria ruminantium, ehrlichia phagocytophila, ehrlichia equi, the human granulocytic ehrlichiosis [hge] agent, anaplasma marginale, anaplasma centrale, ehrlichia sennetsu, ehrlichia risticii, and neorickettsia helminthoeca) have been determined by degenerate pcr and the genome walker method. the ehrlichial ... | 2001 | 11526124 |
| expression of major surface protein 2 variants with conserved t-cell epitopes in anaplasma centrale vaccinates. | major surface protein 2 (msp-2), identified as a protection-inducing immunogen against anaplasma marginale challenge, is an immunodominant outer membrane protein with orthologues in all examined anaplasma species. although immunization with live anaplasma centrale has long been used to induce protection against acute disease upon challenge with virulent a. marginale, its msp-2 structure and whether msp-2 variants are generated during persistence of the vaccine strain was unknown. in this study, ... | 2002 | 11796593 |
| detection of the anaplasma centralevaccine strain and specific differentiation from anaplasma marginale in vaccinated and infected cattle. | bovine anaplasmosis caused by the intraerythrocytic rickettsia anaplasma marginale is the most prevalent tick-borne disease of cattle worldwide. the most efficient method to control anaplasmosis is by vaccination using live anaplasma centrale, a closely related species or subspecies of low pathogenicity that is capable of inducing significant protection against the more virulent a. marginale. in the present study, we applied pcr assays to detect and discriminate field infection with a. marginale ... | 2002 | 12107477 |
| humoral immune response and hematologic evaluation of pregnant jersey cows after vaccination with anaplasma centrale. | the main objective of this work was to evaluate the safety of an anaplasma centrale vaccine in pregnant pure bred jersey cows selected from a herd located at miranda state, venezuela. ten cows of 3-5 months of gestation were chosen and previous vaccination all cows were tested for anaplasma antibodies by the indirect immunofluorescence assay (ifa), so only seronegative cows were included in the group, and for blood parameters, rectal temperature, and pregnancy. selected cows were vaccinated intr ... | 2003 | 12829387 |
| overcoming constraints to meeting increased demand for babesia bigemina vaccine in australia. | demand for live trivalent tick fever vaccine containing babesia bovis, babesia bigemina and anaplasma centrale produced by the department of primary industries, queensland, has increased from less than 10,000 doses in 1988 to 500,000 doses in 2001. this paper describes a series of trials aimed at overcoming certain constraints to obtain b. bigemina parasitised erythrocytes (pes) on a large enough scale from infected splenectomised calves to meet the demand. passage through a series of splenectom ... | 2003 | 12935736 |
| assessment of a low virulence australian isolate of anaplasma marginale for pathogenicity, immunogenicity and transmissibility by boophilus microplus. | a 14-year-old cow (dawn) born and kept in a boophilus microplus-free region gave birth to a calf, which showed the presence of an anaplasma marginale infection after splenectomy. the calf's grand dam was from a b. microplus infected area and we assume the infection originated via the transplacental route over two generations. an isolate, prepared from the calf, had similar or lower pathogenicity as anaplasma centrale, and previously exposed steers were resistant to challenge by four a. marginale ... | 2003 | 14651881 |
| a novel antigen from anaplasma marginale: characterization, expression and preliminary evaluation of the recombinant protein. | through a process of protein fractionation and vaccination we previously identified four native antigens that confer a degree of protection against challenge with anaplasma marginale. one of these, ana 29 has been successfully cloned and sequenced using degenerate primers designed to n-terminal and internal peptide sequences. the full-length gene codes for a protein with a theoretical molecular weight of 27 kda and pi 8.6. the sequence is highly conserved, showing 99% identity between two austra ... | 2004 | 14670322 |
| molecular conservation of msp4 and msp5 in anaplasma marginale and a. centrale vaccine strain. | anaplasma centrale msp4 and msp5 genes were cloned and sequenced, and the recombinant proteins were expressed. the identity between anaplasma marginale and a. centrale msp4 was 83% in the nucleotide sequences and 91.7% in the encoded protein sequences. a. centrale msp5 nucleotide sequences shared 86.8% identity with a. marginale msp5, and there was 92.9% homology between a. centrale and a. marginale encoded amino acids of the msp5 protein. southern blots hybridized with probes derived from the m ... | 2004 | 15135513 |
| amplification of 16s rrna genes of anaplasma species in china for phylogenetic analysis. | in this study, a phylogenetic tree was inferred through comparing five 16s rrna gene sequences of four isolates of anaplasma ovis and one of anaplasma marginale in china with all nineteen 16s rrna gene sequences deposited in genbank (12 a. marginale, 3 a. ovis and 4 anaplasma centrale derived from america, uruguay, south africa, zimbabwe, australia, isreal and japan). the analysis showed that all a. ovis isolated in china were separated into an a. ovis cluster, while the a. marginale in china wa ... | 2005 | 15795086 |
| comparative study of anaplasma parasites in tick carrying buffaloes and cattle. | a comparative study on the prevalence of anaplasma parasite was conducted on ticks carrying buffaloes and cattle. five hundred blood samples of both animals (250 of each) were collected during february, march and april. thin blood smears on glass slides were made, fixed in 100% methyl alcohol and examined. microscopic examination revealed that 205 (41%) animals had anaplasma parasites, out of which 89, 44 and 72 animals had anaplasma marginale, anaplasma centrale and mixed infection respectively ... | 2005 | 16252338 |
| molecular and serological detection of a. centrale- and a. marginale-infected cattle grazing within an endemic area. | a reverse line blot hybridization (rlb) one-stage nested pcr (npcr) for anaplasma centrale and a nested pcr for anaplasma marginale were used to detect infected cattle grazing within an endemic region in israel. a novel set of pcr primers and oligonucleotide probes based on a 16s ribosomal rna gene was designed for rlb detection of both anaplasma species, and the performance of the molecular assays compared. the immunofluorescent antibody test (ifa) was used to detect antibodies to both anaplasm ... | 2006 | 16300909 |
| novel genetic variants of anaplasma phagocytophilum, anaplasma bovis, anaplasma centrale, and a novel ehrlichia sp. in wild deer and ticks on two major islands in japan. | wild deer are one of the important natural reservoir hosts of several species of ehrlichia and anaplasma that cause human ehrlichiosis or anaplasmosis in the united states and europe. the primary aim of the present study was to determine whether and what species of ehrlichia and anaplasma naturally infect deer in japan. blood samples obtained from wild deer on two major japanese islands, hokkaido and honshu, were tested for the presence of ehrlichia and anaplasma by pcr assays and sequencing of ... | 2006 | 16461655 |
| prevalence and genetic diversity of anaplasma marginale strains in cattle in south africa. | bovine anaplasmosis, caused by the tick-borne rickettsia anaplasma marginale, is endemic in south africa and results in considerable economic loss to the cattle industry. this study was designed to characterize strains of a. marginale at the molecular level from cattle raised in communal and commercial farms in the north-eastern and south-western regions of the free state province, south africa, that varied in rainfall and vegetation. seroprevalence to a. marginale was determined in 755 cattle b ... | 2007 | 17359443 |
| detection and quantification of anaplasma marginale dna in blood samples of cattle by real-time pcr. | a taqman-based real-time pcr assay was developed for the diagnosis of anaplasma marginale infection of cattle. the established assay was proven to be highly specific, since no cross-reactions were observed with other anaplasma species of ruminants, including the closely related anaplasma centrale, or other haemoparasites of ruminants (anaplasma bovis, anaplasma ovis, anaplasma phagocytophilum, babesia bovis, babesia bigemina, theileria annulata and theileria buffeli). the detection limit was equ ... | 2007 | 17466470 |
| concomitant infection of cattle with the vaccine strain anaplasma marginale ss centrale and field strains of a. marginale. | bovine anaplasmosis, caused by anaplasma marginale, the intraerythrocytic rickettsia, is controlled by vaccination with live anaplasma marginale ss centrale (a. centrale), a subspecies of relatively low pathogenicity. we have experimentally demonstrated that an animal primarily infected with a. marginale, or with the related vaccine subspecies a. centrale can be infected with the heterologous subspecies, and carries both bacteria. the co-infection was detected in experimentally cross-infected ca ... | 2008 | 18387757 |
| first report of bovine anaplasmosis by anaplasma centrale in europe, molecular identification and phylogenetic analysis. | 2008 | 18696245 | |
| duplex real-time polymerase chain reaction for simultaneous detection and quantification of anaplasma marginale and anaplasma centrale. | anaplasma marginale and anaplasma centrale are rickettsial pathogens responsible for acute disease and mild infections, respectively, in cattle herds. a duplex real-time polymerase chain reaction (pcr) assay with probes labeled with different fluorophores was developed for simultaneous detection and quantification of a. marginale and a. centrale dna in bovine blood samples. the assay was able to detect as few as 10(1) and 10(2) dna copies for a. marginale and a. centrale, respectively, with opti ... | 2008 | 18776093 |
| experimental transmission of field anaplasma marginale and the a. centrale vaccine strain by hyalomma excavatum, rhipicephalus sanguineus and rhipicephalus (boophilus) annulatus ticks. | the cattle rickettsia anaplasma marginale is distributed worldwide and is transmitted by about 20 tick species, but only rhipicephalus simus, a strictly african tick species, has been shown to transmit the vaccine strain of a. centrale. the aim of the present study was to examine transmission of field strains of a. marginale and of the vaccine strain of a. centrale by three tick species -hyalomma excavatum, rhipicephalus sanguineus and rhipicephalus (boophilus) annulatus - to susceptible calves. ... | 2009 | 18823724 |
| first report of bovine anaplasmosis caused by anaplasma centrale in europe. | anaplasma centrale (rickettsiales: anaplasmataceae) is used as a live vaccine for cattle against the pathogenic anaplasma marginale in tropical and subtropical areas. herein we report a clinical case of bovine anaplasmosis associated with a. centrale infection in italy, together with the first molecular identification and phylogenetic analysis of this anaplasma species or subspecies in europe. | 2008 | 19120185 |
| independence of anaplasma marginale strains with high and low transmission efficiencies in the tick vector following simultaneous acquisition by feeding on a superinfected mammalian reservoir host. | strain superinfection occurs when a second pathogen strain infects a host already carrying a primary strain. anaplasma marginale superinfection occurs when the second strain carries a variant repertoire different from that of the primary strain, and the epidemiologic consequences depend on the relative efficiencies of tick-borne transmission of the two strains. following strain superinfection in the reservoir host, we tested whether the presence of two a. marginale (sensu lato) strains that diff ... | 2009 | 19188360 |
| viability assays of intra-erythrocytic organisms using fluorescent dyes. | three intra-erythrocytic tick fever organisms of cattle (babesia bovis, babesia bigemina and anaplasma centrale) were subjected to a range of stressors, including heat, storage over time, specific chemotherapy and cryopreservation. various stains, both alone and in combination, were used in an attempt to assess viability of these organisms before and after the stressors were applied. carboxyfluorescein diacetate succinimidyl ester (cfse) stained live babesia spp. very well while fluorescein diac ... | 2009 | 19380205 |
| complete genome sequence of anaplasma marginale subsp. centrale. | anaplasma marginale subsp. centrale is a naturally attenuated subtype that has been used as a vaccine for a century. we sequenced the genome of this organism and compared it to those of virulent senso stricto a. marginale strains. the comparison markedly narrows the number of outer membrane protein candidates for development of a safer inactivated vaccine and provides insight into the diversity among strains of senso lato a. marginale. | 2010 | 19854912 |
| sir arnold theiler and the discovery of anaplasmosis: a centennial perspective. | sir arnold theiler's research in 1908/09 led to the discovery of the first rickettsial pathogen, anaplasma marginale, and set the stage for his development and implementation of an effective live vaccine based on a less virulent strain, a. marginale ss. centrale. his 1910 report, describing a. marginale, is among the classic monographs in infectious disease research, presenting not only observations in exacting detail but also highlighting the deductive reasoning leading to association of a new ... | 2009 | 19967931 |
| identification of anaplasma centrale major surface protein-2 pseudogenes. | the present study was aimed to identify msp2 pseudogenes and msp2 variants in the vaccine anaplama centrale strain. five msp2 pseudogenes were identified in the a. centrale genome, and multiple msp2 variants that emerged during both acute and persistent infection were detected. the pseudogene copies of msp2 were truncated; they contained a central hypervariable region flanked by short portions of the 5' and 3' conserved regions. alignment of the hypervariable region sequence of the expression si ... | 2010 | 20036077 |
| anaplasma spp. in wild mammals and ixodes ricinus from the north of spain. | our objectives were to investigate the presence of anaplasma spp. infection in red deer, wild boars, and ixodes ricinus removed from deer surveyed in la rioja, as well as to analyze the presence of anaplasma spp. in i. ricinus from different spanish regions--ours included. a total of 21 deer and 13 wild boar blood samples as well as 295 i. ricinus removed from deer, vegetation, or asymptomatic people were tested by polymerase chain reaction targeting anaplasma spp. 16s rrna gene and groesl heat ... | 2011 | 20528172 |
| theileria parva genetic diversity and haemoparasite prevalence in cattle and wildlife in and around lake mburo national park in uganda. | wildlife, especially cape buffalo (syncerus caffer), are thought to act as a reservoir for many of the important tick-borne pathogens of cattle. in this study, we have determined the prevalence of the most significant tick-borne haemoparasites in wildlife (buffalo, impala, eland and bushbuck) as well as in cattle grazing inside and neighbouring lake mburo national park (lmnp) in uganda. a high percentage of buffalo were carriers of theileria parva, theileria mutans, theileria velifera, theileria ... | 2011 | 20827491 |
| haemoparasite prevalence and theileria parva strain diversity in cape buffalo (syncerus caffer) in uganda. | cape buffalo (syncerus caffer) are considered to be an important reservoir for various tick-borne haemoparasites of veterinary importance. in this study we have compared the haemoparasite carrier prevalence in buffalo from four geographically isolated national parks in uganda [lake mburo national park (lmnp), queen elizabeth national park (qenp), murchison falls national park (mfnp) and kidepo valley national park (kvnp)]. differences were seen in haemoparasite prevalence in buffalo from the fou ... | 2010 | 21074945 |
| identification of anaplasma marginale outer membrane protein antigens conserved between a. marginale sensu stricto strains and the live a. marginale subsp. centrale vaccine. | live vaccination with anaplasma marginale subsp. centrale (synonym for anaplasma centrale) induces protection against severe disease upon challenge with a. marginale sensu stricto strains. despite over a century of field use, the targets of protective immunity remained unknown. using a broad proteomic approach, we identified the proteins in a challenge sensu stricto strain that were bound by the relevant antibody isotype induced by live vaccination with anaplasma marginale subsp. centrale. a cor ... | 2010 | 21189322 |
| detection of anaplasma phagocytophilum from wild boars and deer in japan. | this study was conducted to determine the prevalence and geographic distribution of anaplasma phagocytophilum, the causative agent of human granulocytic anaplasmosis, in wild deer and boars in japan. we analyzed the blood samples of the study animals using pcr-targeting the 16s rdna followed by dna sequencing. wild deer infected with anaplasma spp., including anaplasma bovis and anaplasma centrale were detected in the region from hokkaido to kyushu. the infection rates of a. phagocytophilum, a. ... | 2011 | 21788712 |
| identification of multilocus genetic heterogeneity in anaplasma marginale subsp. centrale and its restriction following tick-borne transmission. | anaplasma marginale subsp. centrale was the first vaccine used to protect against a rickettsial disease and is still in widespread use a century later. as its use preceded development of either cryopreservation or cell culture, the vaccine strain was maintained for decades by sequential passage among donor animals, excluding the natural tick-borne transmission cycle that provides a selective pressure or population "bottleneck." we demonstrated that the vaccine strain is genetically heterogeneous ... | 2013 | 23509140 |
| [prevalence of anaplasma spp. infection in a desert landscape region of heshuo, xinjiang]. | to study the prevalence of anaplasmosis among human, domestic sheep and tick population in heshuo area, southern xinjiang and to investigate the diversity of anaplasma species. | 2013 | 23751470 |
| cross-protection between geographically distinct anaplasma marginale isolates appears to be constrained by limited antibody responses. | the rickettsia anaplasma marginale causes the haemolytic disease bovine anaplasmosis, an economic problem in tropical and subtropical areas worldwide. the closely related but less pathogenic anaplasma centrale is commonly used as a live vaccine to prevent anaplasmosis, but it can only be produced from infected blood. ufmg1 is a low pathogenic brazilian strain of a. marginale, which has been shown to protect cattle against a high pathogenic brazilian isolate. as ufmg1 can be grown in tick cells, ... | 2013 | 24589108 |
| retrospective study of hemoparasites in cattle in southern italy by reverse line blot hybridization. | tick-borne diseases are widespread in tropical and temperate regions and are responsible for important economic losses in those areas. in order to assess the presence and prevalence of various pathogens in southern italy, we retrospectively analyzed cattle blood samples collected for a previous study in 2000 using reverse line blot (rlb) hybridization. the study had been carried out in three regions of southern italy on 1,500 randomly selected and apparently healthy adult cattle. rlb showed that ... | 2014 | 24614604 |
| co-circulation of multiple species of rickettsiales bacteria in one single species of hard ticks in shenyang, china. | bacteria in the order rickettsiales include some of the most important zoonotic (re)emerging pathogens for animals and humans. in 2012, a total of 1267 adult haemaphysalis longicornis ticks were collected from domestic animals (cattle and sheep) in shenyang of liaoning province, china. these ticks were grouped into 181 pools (each pool with 6-7 ticks). rickettsiales agents were identified in 93 (51.38%) tick pools using pcr targeting rrs (16s rrna). in addition to 16s rrna gene sequences, glta a ... | 2014 | 25131152 |
| evaluation of the immune response to anaplasma marginale msp5 protein using a hsv-1 amplicon vector system or recombinant protein. | anaplasma marginale is an intraerythrocytic vector-borne infectious agent of cattle. immunization with the current vaccine, based on parasitized erythrocytes with live anaplasma centrale, shows some constraints and confers partial protection, suggesting the feasibility for the development of new generation of vaccines. the aim of the present study was to assess the effect of sequential immunization of balb/c mice, with herpesvirus amplicon vector-based vaccines combined with protein-based vaccin ... | 2014 | 25458492 |
| tick-borne haemoparasites in african buffalo (syncerus caffer) from two wildlife areas in northern botswana. | the african buffalo (syncerus caffer) is a host for many pathogens known to cause economically important diseases and is often considered an important reservoir for livestock diseases. theileriosis, heartwater, babesiosis and anaplasmosis are considered the most important tick-borne diseases of livestock in sub-saharan africa, resulting in extensive economic losses to livestock farmers in endemic areas. information on the distribution of tick-borne diseases and ticks is scarce in northern botswa ... | 2015 | 25589064 |
| development of a new pcr-based assay to detect anaplasmataceae and the first report of anaplasma phagocytophilum and anaplasma platys in cattle from algeria. | bovine anaplasmosis is a hemoparasitic disease considered as a major constraint to cattle production in many countries. this pathology is at least partially caused by anaplasma phagocytophilum, anaplasma marginale, anaplasma centrale, and anaplasma bovis. the global threat and emergence of these species in animals require the reliable identification of these bacteria in animal samples. in this study, we developed a new qpcr tool targeting the 23s rrna gene for the detection of anaplasmataceae ba ... | 2015 | 25748051 |
| molecular survey and sequence analysis of anaplasma spp. in cattle and ticks in a malaysian farm. | this study was conducted to determine the occurrence of anaplasma spp. in the blood samples of cattle, goats, deer and ticks in a malaysian farm. using polymerase chain reaction (pcr) and sequencing approach, anaplasma spp. was detected from 81(84.4%) of 96 cattle blood samples. all blood samples from 23 goats and 22 deer tested were negative. based on the analysis of the anaplasma partial 16s ribosomal rna gene, four sequence types (genotypes 1 to 4) were identified in this study. genotypes 1-3 ... | 2014 | 25776603 |
| bovine anaplasmosis in turkey: first laboratory confirmed clinical cases caused by anaplasma phagocytophilum. | anaplasma species are obligate intracellular rickettsial pathogens that affect the health of humans and other animals. clinical cases of anaplasmosis caused by anaplasma phagocytophilum were evaluated, and the frequency of bovine anaplasma species was determined in cattle. blood samples and thin blood smears were collected from 10 cattle exhibiting clinical signs of tick-borne fever. in addition, blood samples were collected from 123 apparently healthy cattle from the same area. dna was screened ... | 2015 | 26051478 |
| detection of tick-borne anaplasma bovis, anaplasma phagocytophilum and anaplasma centrale in spain. | the genus anaplasma (rickettsiales: anaplasmataceae) includes species of medical and veterinary importance. the presence of anaplasma spp. in ticks from birds, as well as in haemaphysalis punctata (ixodida: ixodidae) specimens collected from cattle and vegetation in northern spain was investigated. a total of 336 ticks from birds [174 ixodes frontalis (ixodida: ixodidae), 108 h. punctata, 34 hyalomma marginatum (ixodida: ixodidae), 17 ixodes ricinus (ixodida: ixodidae) and three ixodes spp.], an ... | 2015 | 26111122 |
| first molecular survey and novel genetic variants' identification of anaplasma marginale, a. centrale and a. bovis in cattle from tunisia. | few data are available about the presence and distribution of anaplasma species in cattle in north african countries. in this study prevalence, co-infections, risk factors and genetic diversity of anaplasma species were evaluated in bovines from northern tunisia. a total of 232 cattle from 36 randomly selected farms in three tunisian localities were investigated for the presence of anaplasma species in blood by real-time pcr and/or nested pcr. overall infection rates of anaplasma spp., anaplasma ... | 2015 | 26117444 |
| propagation of the israeli vaccine strain of anaplasma centrale in tick cell lines. | anaplasma centrale has been used in cattle as a live blood vaccine against the more pathogenic anaplasma marginale for over 100 years. while a. marginale can be propagated in vitro in tick cell lines, facilitating studies on antigen production, immunisation and vector-pathogen interaction, to date there has been no in vitro culture system for a. centrale. in the present study, 25 cell lines derived from 13 ixodid tick species were inoculated with the israeli vaccine strain of a. centrale and mon ... | 2015 | 26210950 |
| anaplasmataceae in wild ungulates and carnivores in northern spain. | wild vertebrates are essential hosts for tick-borne diseases but data on the prevalence and diversity of anaplasma spp. in wildlife are scarce. in this study, we used real-time pcr to investigate the distribution of anaplasma species in spleen samples collected from 625 wild animals (137 cervids, 227 wild boar, and 261 carnivores) in two regions in northern spain. a first generic real-time pcr assay was used to screen for the presence of anaplasma spp. followed by a second species-specific multi ... | 2016 | 26596894 |
| multiple pathogens including potential new species in tick vectors in côte d'ivoire. | our study aimed to assess the presence of different pathogens in ticks collected in two regions in côte d'ivoire. | 2016 | 26771308 |
| molecular detection of tick-borne rickettsiales in goats and sheep from southeastern china. | members from rickettsiales such as anaplasma phagocytophilum, ehrlichia chaffeensis, and some spotted fever group (sfg) rickettsiae are important tick-borne pathogens. one hundred goats and sheep from southeastern china were examined for the presence of anaplasma, e. chaffeensis, and sfg rickettsiae by pcr. a. phagocytophilum, anaplasma bovis, and anaplasma centrale were detected in 15, 49, and 16 samples, respectively. the a. phagocytophilum and a. centrale were highly homologous to strains fro ... | 2016 | 26872274 |
| tick-borne pathogens of zoonotic and veterinary importance in nigerian cattle. | ticks and tick-borne diseases undermine cattle fitness and productivity in the whole of sub-saharan africa, including nigeria. in this west african country, cattle are challenged by numerous tick species, especially during the wet season. consequently, several tbds are known to be endemic in nigerian cattle, including anaplasmosis, babesiosis, cowdriosis and theilerioris (by theileria mutans and theileria velifera). to date, all investigations on cattle tbds in nigeria have been based on cytolog ... | 2016 | 27090756 |
| anaplasma marginale and theileria annulata in questing ticks from portugal. | ticks are ubiquitous arthropods and vectors of several pathogenic agents in animals and humans. monitoring questing ticks is of great importance to ascertain the occurrence of pathogens and the potential vector species, offering an insight into the risk of disease transmission in a given area. in this study 428 host-seeking ticks, belonging to nine species of ixodidae and collected from 17 of the 23 portuguese mainland subregions, were screened for several tick-borne agents with veterinary relev ... | 2016 | 27394441 |
| characterization of anaplasma marginale subsp. centrale strains by use of msp1as genotyping reveals a wildlife reservoir. | bovine anaplasmosis caused by the intraerythrocytic rickettsial pathogen anaplasma marginale is endemic in south africa. anaplasma marginale subspecies centrale also infects cattle; however, it causes a milder form of anaplasmosis and is used as a live vaccine against a. marginale there has been less interest in the epidemiology of a. marginale subsp. centrale, and, as a result, there are few reports detecting natural infections of this organism. when detected in cattle, it is often assumed that ... | 2016 | 27440819 |
| molecular diagnosis and genetic diversity of tick-borne anaplasmataceae agents infecting the african buffalo syncerus caffer from marromeu reserve in mozambique. | tick-borne diseases (tbds) are very important in relation to domestic ruminants, but their occurrence among wild ruminants, mainly in the african buffalo syncerus caffer, remains little known. | 2016 | 27531003 |
| identification of a t-cell epitope that is globally conserved among outer membrane proteins (omps) omp7, omp8, and omp9 of anaplasma marginale strains and with omp7 from the a. marginale subsp. centrale vaccine strain. | within the protective outer membrane (om) fraction of anaplasma marginale, several vaccine candidates have emerged, including a family of om proteins (omps) 7 to 9, which share sequence identity with each other and with the single protein omp7 in the vaccine strain a. marginale subsp. centrale. a. marginale omps 7 to 9 are logical vaccine candidates because they are surface exposed, present in the om immunogen and protective cross-linked om proteins, recognized by immune serum igg2 and t cells i ... | 2017 | 27795302 |
| guidelines for the direct detection of anaplasma spp. in diagnosis and epidemiological studies. | the genus anaplasma (rickettsiales: anaplasmataceae) comprises obligate intracellular gram-negative bacteria that are mainly transmitted by ticks, and currently includes six species: anaplasma bovis, anaplasma centrale, anaplasma marginale, anaplasma phagocytophilum, anaplasma platys, and anaplasma ovis. these have long been known as etiological agents of veterinary diseases that affect domestic and wild animals worldwide. a zoonotic role has been recognized for a. phagocytophilum, but other spe ... | 2017 | 28055579 |
| comparison of three nucleic acid-based tests for detecting <i>anaplasma marginale</i> and <i>anaplasma centrale</i> in cattle. | several nucleic acid-based assays have been developed for detecting anaplasma marginale and anaplasma centrale in vectors and hosts, making the choice of method to use in endemic areas difficult. we evaluated the ability of the reverse line blot (rlb) hybridisation assay, two nested polymerase chain reaction (npcr) assays and a duplex real-time quantitative polymerase chain reaction (qpcr) assay to detect a. marginale and a. centrale infections in cattle (n = 66) in south africa. the lowest dete ... | 2017 | 28155283 |
| molecular detection and characterization of anaplasma species in wild deer and boars in gifu prefecture, japan. | 2017 | 28250258 |