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haemophilus infections in chickens. iv. results of laboratory and fieldtrials of formalinized bacterins for the prevention of disease caused by haemophilus gallinarum. 19635888520
application of the agar gel precipitation test to serological studies of chickens inoculated with haemophilus gallinarum. 19655855815
infectious coryza of chickens. v. influence of mycoplasma gallisepticum infection on chicken infected with haemophilus gallinarum. 19655871448
nucleic acid homologies of selected bacteria, l forms, mycoplasma species.rogul, m. (walter reed army institute of research, washington, d.c.), z. a. mcgee, r. g. wittler, and stanley falkow. nucleic acid homologies of selected bacteria, l forms, and mycoplasma species. j. bacteriol. 90:1200-1204. 1965.-the molar per cent of guanine plus cytosine (g + c) in the deoxyribonucleic acids (dna) of proteus mirabilis, strain 9, and its stable l form was determined by thermal denaturation and found to be approximately 39.5% g + c. the dna homologies of this bacterium and its ...19655848325
synergism between avian infectious bronchitis virus and haemophilus gallinarum. 19674962423
identification of haemophilus gallinarum in artificially infected chickens. 19684868834
the field trial: some thoughts on the indispensable ordeal. 19684875751
antibody deficiency syndrome and autoimmune haemolytic anaemia in a boy with isolated igm deficiency dysimmunoglobulinaemia type 5. 19694188208
antibacterial activities of new nitrofuran derivatives on mycoplasm gallisepticum and haemophilus gallinarum. 19704191833
effect of non-narcotic analgesics on anticoagulant-induced hypoprothrombinemia in rats. 19724120193
[response of the recipient's tissue bed and features of reparative osteogenesis in retransplantation of bone tissue]. 19724553209
[spinal and spino-bulbo-spinal neuronal mechanisms of transmitting somato- and viscero-motor influences in the thoracic region of the spinal cord]. 19734777797
[report on the differentiation of haemophilus isolates from chickens. i. note: cultural and biochemical studies].thirty-one isolates of haemophilus organismus, most of them isolated from respiratory organs of sick chickens, and 3 haemophilus gallinarum strains from california (usa) were culturally and biochemically investigated. two groups of haemophilus strains were differentiated. of the 23 isolates belonging to group i, 22 were obtained from chickens with coryza contagiosa gallinarum. one strain was isolated from the eye of a sick chicken. these microaerophilic strains required the v factor only and fai ...197318777398
a growth medium for the production of a bacterin for immunization against infectious coryza.a serum-free modified casman broth medium was developed to grow haemophilus gallinarum to titers of 10-8 organisms per ml. formalin-inactivated bacterins prepared form the broth culture were used to immunize chickens. a single dose of bacterin containing adjuvant protected 6 of 7 chickens against a homologous challenge 4 weeks postvaccination. a second dose given 3 weeks after the first offered no better protection than a single dose.19751156259
enhancement of hemagglutinating activity of haemophilus gallinarum by trypsin.it is shown that hemagglutination (ha) activity of haemophilus gallinarum is enhanced by trypsin. ha activation was more effective at 37 c than at room temperature.1976962767
further observations on the use of a bivalent bacterin against haemophilus gallinarum.chickens vaccinated with two doses of a bivalent haemophilus gallinarum bacterin were protected against seven strains of the organism. vaccinated and unvaccinated birds with antibody to mycoplasma gallisepticum had increased hi titers when challenged with h. gallinarum. birds positive for antibody to mycoplasma gallisepticum which were not challenged showed no increase in hi titer.1976962761
the effect of atmospheric conditions on the growth of haemophilus gallinarum in a defined medium. 19761255134
efficacy studies on haemophilus gallinarum bacterin preparations.broth cultures inactivated and potentiated by selected methods were tested in chickens for efficacy against homologous and heterologous challenge inoculation, using 2 serotype a strains of haemophilus gallinarum. although the 2 strains were within the same serotype, they failed to cross protect. one dose of thimerosal-inactivated bacterin was protective against homologous challenge, but 2 doses of formalin-inactivated bacterin were required. a bivalent bacterin protected chickens well against 1 ...19761259216
restriction endonuclease from haemophilus gallinarum (hgai) cleaves polyoma dna at four locations.a restriction endonuclease obtained from haemophilus gallinarum (hgai) cleaves polyoma dna at four specific sites. using the ecori, hindiii, and hpaii endonuclease restriction sites as reference, the four hgai cleavage sites were mapped at 0.02, 0.14, 0.27, and 0.48 fractional lengths, clockwise, from the single ecori cleavage site.1976178910
[differentiation of haemophilus strains isolated from chickens. iv. studies on the dissociation of haemophilus paragallinarum].bacteria of 2 phenotypic forms of one strain of haemophilus paragallinarum were morphologically, culturally, biochemically and serologically investigated. on transparent solid media bacteria of the m variant (mucoid) were capsulated and showed iridescence in oblique transmitted light throughout the first 8-14 hours of incubation. iridescence disappeared completely after 36 hours incubation. bacteria of this variant formed stable homogeneous suspensions in 0.15 m nacl and an homogeneous cloudines ...197618777329
infectious coryza: in vivo growth of haemophilus gallinarum as a determinant for cross protection.four strains of haemophilus gallinarum representing 3 immunotypes were used in exposure and challenge exposure studies to determine if in vivo growth of the organism would induce cross protection in chickens. birds which recovered from infection to 1 immunotype were refractory to reinfection with the heterologous immunotype. in contrast, in vitro-produced bacterins produced immunotype-specific protection.1977931141
cleavage specificity of the restriction endonuclease isolated from haemophilus gallinarum (hga i).the nucleotide sequences in the replicative form (duplex) of phix174 dna around six sites cut by hga i, a restriction endonuclease from haemophilus gallinarum, have been compared. the enzyme produces a staggered cleavage resulting in a pentanucleotide 5'-terminal extension. the sequences within and immediately surrounding the pentanucleotide cleavage site have no obvious relationship. however, the sequence 5'-g-a-c-g-c-3' 3'-c-t-g-c-g-5' occurs five nucleotide pairs to the left of the cut in th ...1977269384
serologic response to haemophilus gallinarum in artifically infected and vaccinated chickens.serologic responses to haemophilus gallinarum (hg) were compared in chickens artifically inoculated with living hg and vaccinated with hg bacterin, using tube agglutination (agg), hemagglutination-inhibition (hi), and agar-gel precipitation (agp) tests. infected and vaccinated chickens differed markedly, as did infection routes early after treatment. hi response was delayed and lower in chickens infected intranasally than in vaccinated chickens. both agg and agp responses peaked sooner than hi. ...1977402906
infectious coryza: cross-protection studies, using seven strains of haemophilus gallinarum.bacterins prepared from 7 strains of haemophilus gallinarum were used to immunized chickens for cross-protection studies. three distinct immunotypes were distinguished. slight protection between immunotypes was evident for some strains. airsacculitis could be prevented by use of these bacterins; however, prevention was related to immunotype specificity. hyaluronic acid found in 2 strains rendered them inagglutinable in homologous antiserums. treatment with hyaluronidase rendered them agglutinabl ...1977931140
experimental coryza in broiler chickens. i. effects of vaccination with haemophilus gallinarum bacterin and its components on weight gains and resistance to infection.effects of a haemophilus gallinarum bacterin and its components were studied in young broiler chickens. when the bacterin was administered subcutaneously in the dorsal neck region at 2 weeks of age, no significant differences in weight gains of vaccinated and control birds were detected at eight weeks of age. in four groups vaccinated at 1, 2, 3, or 4 weeks of age and challenged with virulent haemophilus organisms 3 weeks later, the incidence of clinical signs was 30% in the vaccinates and 60% i ...1977907579
production and properties of hemagglutinin of haemophilus gallinarum.production of hemagglutinin (ha) of haemophilus gallinarum was compared in some media, and its properties were studied. ha was produced in kato's media, brain heart infusion (bhi) broth containing beta-diphosphopyridine nucleotide, and chicken meat infusion (cmi) broth. the ha in cmi broth different according to the concentration of the chicken serum; no ha titer was found in 0.5% or more chicken serum, but ha was activated by storage in a refrigerator. cells of h. gallinarum cultured for a long ...1977843302
[the occurrence of neuraminidase and n-acylneuraminate pyruvate lyase in haemophilus paragallinarum and haemophilus paravium n.sp (author's transl)].14 strains of h. paragallinarum, isolated from fowls suffering from infectious coryza or received from other investigators for comparison, and a collective consisting of 9 strains of the new species h. paravium nova species, also isolated from fowls, were shown to possess both enzymes, neuraminidase (ec 3.2.1.18) and n-acylneuraminate-pyruvate lyase (ec 4.1.3.3). the detection of the enzymes was made initially by paper chromatography using n-acetyl-neuraminyllactose as substrate and secondly by ...1977842179
psychological, physiological, and pharmacological management of pain.it has been the intent of the authors to impress upon the clinician the multifaceted nature of the human pain experience. we have sought to create an understanding of the emotional nature of pain. the treatment of pain as a pure sensation without directing attention to its behavioral and psychological aspects will generally lead to failure in understanding and ultimately in treating this ubiquitous problem in our profession. the use of drugs alone will have only a minor effect on the treatment o ...1977267605
the effect of lymphoma and other neoplasms on hepatic and plasma enzymes of the host rat.considerable thymidine kinase and pyrroline-5-carboxylate reductase activities were found in the plasma of rats bearing a transplanted lymphoma; neither activity was detected in plasma of hosts carrying hepatic, renal, mammary, or submaxillary gland tumors. all host livers exhibited signs of biochemical immaturity as indicated by the appropriate increases or decreases in the concentrations of the nine enzymes measured. the extent and time schedule of the changes in host liver varied with the enz ...1977187334
suppression of immunoresponses to haemophilus gallinarum with nonviable mycoplasma gallisepticum in chickens.the suppressive effect of mycoplasma gallisepticum (mg) on haemophilus gallinarum (hg) immune response was shown. antibody response to hg was highly suppressed when chickens were inoculated intramuscularly with hg-mg combined bacterin. findings were similar in chickens injected intramuscularly with hg and mg bacterin separately at adjacent sites. no immunosuppressive effect was recognized when injections with hg and mg bacterins were in the left and right thigh muscles, respectively, or from int ...1978749885
slide-agglutination test of haemophilus gallinarum antigen treated by trypsin to inhibit spontaneous agglutination.spontaneous agglutination of haemophilus gallinarum (hg) cells cultured in kato's broth was inhibited by trypsin treatment. the treated cell had the same antigenicity as the common antigen prepared from chicken meat infusion broth. trypsinized antigen could be used in the slide-agglutination test to detect hg antibody in chicken serum.1978749897
production of a chicken meat infusion broth suitable for the mass production of a haemophilus gallinarum bacterin.a chicken meat infusion broth was prepared by various formulations and tested in 200 l volumes for their suitability to support the growth of haemophilus gallinarum. the best yield (0,1% packed cells, or 10(9) colony-forming units/ml) was obtained with a medium prepared from whole flayed carcasses with a ratio of 1 kg of meat to 1,2 l of water.1978572951
septo-epithalamo-habenular lrf-reactive neurons in monkeys. 197898207
haemophilus infections in chickens. i. characterization of haemophilus paragallinarum isolated from chickens affected with coryza. 1978565424
recognition sequence of a restriction endonuclease from haemophilus gallinarum.from comparison of the sequences in and around the cleavage sites of restriction endonuclease hgai isolated from haemophilus gallinarum, the recognition sequence and cleavage site of this enzyme was deduced as below: (formula: see text) this enzyme recognizes a specific but asymmetric penta-nucleotide sequence, gcgtc, and introduces staggered cleavages at appointed positions away from the recognition sequence, generating protruding 5'-ends of five nucleotides. the sequences surrounding the cleav ...1978631561
infectious coryza: preventing complicated coryza with haemophilus gallinarum and mycoplasma gallisepticum bacterins.three types of infectious coryza were produced in unvaccinated chickens by challenge inoculums containing different combinations of haemophilus gallinarum (hg) and mycoplasma gallisepticum (mg). monovalent and combination bacterins of hg and mg were tested to determine their efficacy against chronic complicated infectious coryza. challenge exposure of vaccinates with mg and hg showed protection against the hg component to be immunotype-specific. some protection against complicated coryza resulte ...1978646755
production of extracellular antigen in culture supernate by haemophilus gallinarum. 1978670439
haemophilus infections in chickens. 2. types of haemophilus paragallinarum isolates from chickens with infectious coryza, in relation to haemophilus gallinarum strain no. 221. 1978723100
experimental and postexperimental effects of posteriorly directed extraoral traction in adult macaca fascicularis.the experimental, postexperimental, and postretention effects of continuous high-pull headgear force application to the maxilla were evaluated in four adult, nongrowing macaca fascicularis monkeys. force was applied at 450 grams per side to face-bows attached to cast maxillary splints with an implanted occipital plug for anchorage. the active experimental phase lasted from 84 days to 205 days, and its effects were documented histologically, cephalometrically, and with dry skull preparations. pos ...1979107807
the use of haemophilus gallinarum dna-relaxing enzyme to investigate the relationship between the number of superhelical turns and the molecular weight in a negatively twisted dna.covalently closed-circular, superhelical dnas, including viral dnas, bacterial plasmid dnas, and bacteriophage replicative-form dna, were treated with a small amount of haemophilus gallinarum dna-relaxing enzyme to generate incompletely relaxed dna molecules. each sample consisted of a set of closed-circular dna molecules differing by one turn in their number of superhelical turns. the dna samples were analyzed by agarose gel electrophoresis under conditions such that the electrophoretic mobilit ...1979229100
the activity of levamisole against benzimidazole resistant haemonchus contortus and trichostrongylus colubriformis. 1979516343
difference of chicken red blood cells in susceptibility to haemophilus paragallinarum hemagglutinin. 1979480772
separation with trypsin of hemagglutinin of haemophilus paragallinarum. 1979430932
antigenic structure and relationship between serotypes 1 and 2 of haemophilus paragallinarum.antigenic structure and relationship between serotypes 1 and 2 of haemophilus paragallinarum were analyzed by the rapid plate agglutination and cross-absorption tests. encapsulated strain forming iridescent colony type of both serotypes 1 and 2 had at least three antigens: heat-labile and trypsin-sensitive (l), heat-labile and trypsin-resistant (hl), and heat-stable and trypsin-resistant (hs). the l was a major antigen located in a surface and divided serologically into three parts: l1, l2, and ...197993424
purification and characterization of dna-relaxing enzyme from haemophilus gallinarium.a dna-relaxing enzyme capable of concerted nicking and closing of dna backbone bonds has been purified from haemophilus gallinarum by two chromatographic steps and gel filtration. the enzyme efficiently catalyzes the removal of superhelical turns from a negatively twisted dna and requires mg2+ for this activity. slight removal of superhelical turns from a positively twisted dna generated by binding of ethidium bromide is found, but only at high enzyme concentrations. the dna-relaxing activity is ...1979227461
synthetic antigens iii. antigen-binding cells to interpolymer of styrene and maleic acid psm and sheep red blood cells in mice immunized with psm.studies on the immune response in mice to a new synthetic antigen interpolymer of styrene and maleic acid (psm) are described. the immune response was measured by determination of antigen binding cells by means of rosette test with psm-coated srbc, an unexpected effect was observed--besides rosette-forming cells for psm, rosette-forming cells and agglutinating antibodies for sheep red cells were found. the results presented in this paper suggest that psm is acting both as an immunogen and a poly ...197994989
heat-stable antigenic determinants of haemophilus paragallinarum. 19806162301
performance of 3 successive generations of specified-pathogenfree chickens maintained as a closed flock.no antibodies against salmonella pullorum, mycoplasma gallisepticum, mycoplasma synoviae, haemophilus gallinarum, fowl pox virus, marek's disease virus, herpes virus of turkey, infectious laryngotracheitis virus, avian adenovirus, avian reovirus, infectious bursal disease virus, reticuloendotheliosis virus, avian leukosis virus, avian encephalomyelitis virus and newcastle disease virus were detectable in the sera obtained from these chickens in 3 generations at various ages. antibodies against i ...19806253742
relationship between protective activity and antigen structure of haemophilus paragallinarum serotypes 1 and 2.the object in this investigation was to determine the relationship between protective activity and antigenic structure of haemophilus paragallinarum, serotypes 1 and 2. a close relationship exists in both serotypes between protective activity and colonial phenotypic form (iridescent and noniridescent). protective activities of both serotypes were related to a heat-labile, trypsin-sensitive (l) antigen of iridescent form that produced serotype-specific agglutinin to chickens. the chickens having ...19806153875
immunologic relationship between page's and sawata's serotype strains of haemophilus paragallinarum.immunologic relationship among page's a, b, and c and sawata's 1 and 2 serotype strains of haemophilus paragallinarum was investigated. in cross-protection tests, two distinct type-specific immunities existed among the strains used. all page's serotype a strains (083, w, georgia, and germany) were immunologically similar to sawata's serotype 1 strain 221, although serotype c strain modesto was similar to serotype 2 strain h-18. cross-protection was not observed between these two serotypes. howev ...19807406296
biologic and serologic relationships between page's and sawata's serotypes of haemophilus paragallinarum.biologic and serologic relationships between page's serotypes a, b, and c and sawata's serotypes 1 and 2 of haemophilus paragallinarum were investigated. of the 7 page's serotype strains testes (which were biologically confirmed as h paragallinarum), serotype b strains spross and 0222 (which produced a low-iridescent smooth-type colony) required v factor, but did not require chicken serum for growth. serotype a strains 083, w, georgia, and germany and serotype c strain modesto, as well as sawata ...19807212424
purification and properties of haemophilus paragallinarum hemagglutinin.hemagglutinin (ha) of haemophilus paragallinarum was purified, and its immunologic, chemical, and morphologic properties were studied. the ha was purified by gel filtration on sepharose 6b and by subsequent ultracentrifugation of trypsinized cells after pretreatment with neuraminidase. after gel filtration, the ha component (fractioned ha) gave 1 precipitin line by gel diffusion, and after inoculation in chickens, hemagglutinination-inhibition antibody to trypsin-sensitive ha antigen was found. ...19807212446
properties of heat-stable antigen in the culture supernate of haemophilus paragallinarum. 19807230571
haemophilus infections in chickens. 3. immunogenicity of serotypes 1 and 2 strains of haemophilus paragallinarum. 19807230573
occurrence of two hemagglutinins on haemophilus paragallinarum strain 221 and comparison of their properties. 19807230577
serological and immunological differences between two hemagglutinins of haemophilus paragallinarum strain 221. 19807230578
[effect of the neuraminidases of haemophilus avium and haemophilus paragallinarum on chicken serum and other substrates].the activity of neuraminidases of two strains of haemophilus avium and three strains of h. paragallinarum were examined using serum of 1-day-old, 8-week-old and 2-year-old chickens as well as human serum, n-acetyl neuraminosyl-d-lactose (nl), n-acyl neuraminic acid glycoproteid (bsm) and colominic acid (col) as substrates. further the n-acetyl neuraminate (nan) content in chicken and human serum was determined. no significant differences were found between the content of nan in human serum and 1 ...198018770294
latex agglutination test for measurement of type-specific antibody to haemophilus paragallinarum in chickens.a latex agglutination test employing serotype-specific haemophilus paragallinarum polysaccharides as the antigen has been developed. it can be used to measure the antigen-antibody reaction in chicken sera when spontaneous latex particle agglutinin is removed without loss of detectable antibody by kaolin pretreatment. the test was tried to detect the serological response in immunized chickens with two different serotype strains of h. paragallinarum; it could detect the type-specific antibody, and ...19817337616
hemagglutination inhibition of haemophilus paragallinarum type 1 hemagglutinin by lipopolysaccharide. 19817332131
the isolation of haemophilus paragallinarum from japanese quail. 19817340794
use of gonococcal agar medium for preparation of antigen of haemophilus paragallinarum hemagglutinin.some solid media for antigen preparation of haemophilus paragallinarum (hpg) hemagglutinin (ha) were studied. when ha activities of hpg strain 221 cultivated on chicken meat infusion (cmi) and gonococcal (gc) agar media were compared, the titer of the gc agar medium appeared faster and was higher than the titer on the cmi agar medium. for both agar media, the ha titer and growth increased with the concentration of the chicken serum added. in addition, ha activity on gc agar medium showed a highe ...19827092740
further characterization of haemophilus paragallinarum and haemophilus avium.a total of 60 isolates of haemophilus spp. from chickens, including four reference strains of h. paragallinarum and one of h. avium, were examined for their physiological and biochemical properties. the isolates could be placed into two groups. one group was identified as h. paragallinarum and consisted of 43 isolates including the four reference strains of h. paragallinarum. the other group was identified as h. avium and consisted of 17 isolates including the reference strain of h. avium. h. av ...19826758314
hemagglutinin of haemophilus paragallinarum serotype 2 organisms: occurrence and immunologic properties of hemagglutinin.hemagglutinating properties of haemophilus paragallinarum serotype 2 and serotype c against freshly collected and glutaraldehyde (ga)- fixed chicken rbc were investigated. different from serotype 1, the nontreated organisms of serotype 2 and serotype c lacked hemagglutinating activity. however, when the organisms were treated with potassium thiocyanate (kscn) and/or sonication, activity occurred not only against ga-fixed chicken rbc, but also against ga-fixed rbc of various animal species. the m ...19826808871
[presence and incidence of haemophilus paragallinarum in swiss poultry stocks]. 19827100880
adhesion of haemophilus paragallinarum to cultured chicken cells.adhesion of haemophilus paragallinarum to chicken embryo fibroblasts (cef) in vitro was investigated in correlation with its virulence. under the scanning electron microscope, the organisms were seen adhering to cef exposed to the bacteria. by transmission electron microscopy, the organisms appeared to attach to the plasma membrane of cef by their fuzzy material. they were enclosed in membrane-limited vesicles and appeared morphologically intact. specific fluorescence was seen in the cytoplasm o ...19827167062
serological classification of haemophilus paragallinarum with a hemagglutinin system.antigens, prepared from 17 strains of haemophilus paragallinarum by treatment with potassium thiocyanate followed by sonication, uniformly agglutinated glutaraldehyde-fixed chicken erythrocytes and formed specific hemagglutination inhibition antibodies in rabbits. attempts were made to classify the strains into serotypes by a combination of cross-hemagglutination inhibition and cross-absorption tests, using the hemagglutinating antigens, designated as ha-l hemagglutinin, and their antisera. the ...19836874914
[in vivo effectiveness of the sulfonamide sulfachlorpyridazine (scp) and of the combination of sulfachlorpyridazine and trimethoprim (scp + tmp) against haemophilus paragallinarum]. 19836347605
correlation of enzyme-induced cleavage sites on negatively superhelical dna between prokaryotic topoisomerase i and s1 nuclease.negatively superhelical pns1 dna with a molecular weight of 2.55 mda (4 kbp) was found to contain 13 specific, unbasepaired sites that are sensitive to a single-strand-specific s1 nuclease cleavage. the s1-cleavage occurred once at these sites. in the absence of added mg2+, the topoisomerase i purified from haemophilus gallinarum formed a complex with the superhelical pns1 dna which has a hidden strand cleavage. extensive proteinase k digestion of the complex led to cleavage of the dna chain. th ...19836303425
serologic and immunologic studies on three types of hemagglutinin of haemophilus paragallinarum serotype 1 organisms. 19836200636
relationships between virulence and morphological or serological properties of variants dissociated from serotype 1 haemophilus paragallinarum strains.relationships between morphological or serological properties and virulence were investigated in seven haemophilus paragallinarum variants. the variants, originated from five serotype 1 strains, were classified into seven types on the basis of colony morphology and iridescence and the presence of variant-specific antigens. smooth (s) and encapsulated organisms having variant-specific antigens and forming highly iridescent (ir+) colonies were highly virulent in vivo; slightly encapsulated organis ...19836885991
[embryonal liposarcoma of the mandible (a case from practice)]. 1983272783
susceptibility of haemophilus paragallinarum to bactericidal activity of normal and immune chicken sera. 19846521142
clearance of the challenge organisms from the upper respiratory tract of chickens injected with an inactivated haemophilus paragallinarum vaccine. 19846521144
serologic typing of haemophilus paragallinarum based on serum bactericidal reactions. 19846521153
serological classification of australian isolates of haemophilus paragallinarum.thirty-nine australian isolates of haemophilus paragallinarum were compared serologically with 3 reference serotype strains of h. paragallinarum using a plate agglutination test. twenty-eight of the isolates were serotype c, 5 were serotype a, while the remaining 6 isolates could not be assigned to a serotype.19846210076
pathogenicity of australian isolates of haemophilus paragallinarum and haemophilus avium in chickens.twenty-seven australian avian haemophilus isolates were tested for their ability to cause infectious coryza in specific pathogen-free chickens. all 15 isolates, identified as h. paragallinarum, produced infectious coryza, whereas all 12 h. avium isolates were nonpathogenic, but spread to in-contact chickens.19846232754
the effect of oil-emulsion vaccines on the occurrence of nonspecific plate agglutination reactions for mycoplasma gallisepticum and m. synoviae.six groups of ten 18-week-old mycoplasma-free white leghorn pullets were vaccinated with one of the following: mycoplasma gallisepticum (mg) bacterin. haemophilus gallinarum bacterin, pasteurella multocida bacterin, combined infectious bursal disease (ibd)-newcastle (ndv) chicken-embryo-origin (ceo) vaccine. ibd-ndv tissue-culture-origin (tc) vaccine, or saline emulsified in oil; one group received no vaccine. plate agglutination tests for m. synoviae (ms) and mg were done for 10 weeks after vac ...19846331364
relationship between anticapsular antibody and protective activity of a capsular antigen of haemophilus paragallinarum. 19846492537
[clinical cases of infectious coryza and the properties of isolated haemophilus strains].studied were a total of sixteen cases with the clinical picture of coryza. leading role in eleven of them had haemophilus, in one the signs of chronical cholera and pox were seen, and in three there was a pseudomonas aeruginosa infection. all cases were serologically positive for crd. by their needs of nutrient growth factors the isolated haemophilus strains belonged to h. paragallinarum, and they proved pathogenic for 8-week-old birds and poults that were about to lay their first eggs, killing ...19846506456
hemagglutinin of haemophilus paragallinarum serotype 1 organisms. 19846737824
immunologic properties of variants dissociated from serotype 1 haemophilus paragallinarum strains. 19846737827
lesions induced in the respiratory tract of chickens by encapsulated or nonencapsulated variants of haemophilus paragallinarum.lesions induced in chickens by an encapsulated or nonencapsulated strain of haemophilus paragallinarum were investigated. in terms of lesion severity, major differences in pathogenicity were observed between the encapsulated and nonencapsulated variants. the principal lesion manifested by the encapsulated variant was an acute catarrhal inflammation of the upper respiratory tract, mainly of the nasal cavity and paranasal sinus. infiltration of a large number of mast cells into the lamina propria ...19854003895
intranasal inoculation of chickens with encapsulated or nonencapsulated variants of haemophilus paragallinarum: electron microscopic evaluation of the nasal mucosa.chickens were inoculated intranasally with encapsulated or nonencapsulated strains of haemophilus paragallinarum. the nasal mucosa of the chickens were examined, using scanning and transmission electron microscopy. in chickens given the encapsulated variant, marked loss of cilia and microvilli, infiltration of leukocytes, and deposition of a mucopurulent substance were seen on the surface of the nasal mucosa; the number of microvillous cells were markedly increased, and infiltration of mast cell ...19854073645
the resistance to anti-microbial agents of bacteria isolated from pathological conditions of birds in victoria, 1978 to 1983.the resistance to anti-microbial agents of bacteria isolated from pathological conditions of birds in victoria, 1978 to 1983, was determined for isolates of escherichia coli, salmonella species, staphylococcus aureus, pasteurella multocida, p. anatipestifer, yersinia pseudotuberculosis and haemophilus paragallinarum. the isolates of e. coli had a high prevalence of resistance to tetracycline and sulphonamides, and a lower prevalence of resistance to furazolidone and sulphamethoxazole-trimethopri ...19853914884
an evaluation of commercial discs for the determination of the growth factor requirements of avian haemophili.the ability of 2 brands of growth factor discs (oxoid and mast) to identify correctly the growth factor requirements of 41 isolates of haemophilus paragallinarum and 17 isolates of h. avium was evaluated. the percentage of isolates correctly identified as requiring v factor varied with both the brand of the disc and the medium used. on basic nutrient media both brands gave low percentages of correct results: on isosensitest agar (oxoid) the oxoid discs gave 26% and the mast discs 24% while on he ...19853984205
partial purification and characterization of type i dna topoisomerase from bacillus stearothermophilus.type i dna topoisomerase was partially purified from bacillus stearothermophilus by ammonium sulfate precipitation and column chromatographies on phosphocellulose, deae-cellulose and heparin-agarose. on heparin-agarose chromatography, topoisomerase i activity was separated into three fractions (designated fractions a, b, and c). each fraction was further subjected to gel filtration on sephacryl s-200. from electrophoretic analysis on polyacrylamide gel, fraction a was found to contain two enzyme ...19852991208
genetic and phenotypic comparison of three new avian haemophilus-like taxa and of haemophilus paragallinarum biberstein and white 1969 with other members of the family pasteurellaceae pohl 1981.in the course of post-mortem bacteriological examinations, several previously unreported bacterial strains were isolated from budgerigars, pigeons, kestrels, and a goose. they have been separated into three distinct collectives according to their cultural, morphological, and biochemical characteristics. since they require v factors, they were tentatively assigned to the genus haemophilus winslow et al. 1917. this preliminary classification was checked by determination of guanine + cytosine conte ...19854074234
taxonomy of some recently described avian pasteurella/actinobacillus-like organisms as indicated by deoxyribonucleic acid relatedness.members of the family pasteurellaceae pohl 1981 are frequently encountered in birds as parasites or pathogens, e.g. the well-known species pasteurella multocida, pasteurella gallinarum, haemophilus paragallinarum, and three species containing strains that had been previously classified as "haemophilus avium"(pasteurella avium, pasteurella volantium, and an unnamed pasteurella species defined by mutters et al., 1985). a variety of additional taxa which had been tentatively assigned to the family ...198518766923
comparison of adjuvants for an inactivated infectious coryza vaccine.differently formulated inactivated infectious coryza vaccines were administered to 6-week-old chickens as a single dose of 10(8) colony-forming units of haemophilus paragallinarum hp31. after 3 weeks, all chickens were challenged by intrasinus inoculation of hp31. two vaccines, one containing an aluminum-hydroxide adjuvant and the other a combined aluminum-hydroxide + mineral-oil adjuvant, gave the best protection (means of 80% and 90%, respectively). two vaccines that contained mineral oil as t ...19873579795
further efficacy studies on inactivated, aluminum-hydroxide-adsorbed vaccines against infectious coryza.specific-pathogen-free chickens were given infectious coryza vaccines containing inactivated cells of haemophilus paragallinarum adsorbed onto an aluminum-hydroxide gel. the vaccines were protective when given subcutaneously or intramuscularly but not when given intranasally. vaccines prepared with two different commercial brands of aluminum-hydroxide gel gave similar protection. vaccines in which the inactivating agent was thimerosal were more protective than similar vaccines in which the inact ...19872960312
mixed infection with chlamydia psittaci, fowlpox virus and haemophilus gallinarum in broiler breeder chicks. 19873037759
alteration of lung structure and function by pseudomonas aeruginosa. 19873122195
further comparison of adjuvants for an inactivated infectious coryza vaccine.inactivated infectious coryza vaccines containing different adjuvants were administered to 6-week-old chickens as a single dose containing 10(8) colony-forming units of haemophilus paragallinarum hp31. after 3 weeks, all chickens were challenged by intrasinus inoculation of hp31. two vaccines, one containing an aluminum-hydroxide adjuvant and the other a combined aluminum-hydroxide + avridine (a lipoidal amine) adjuvant, were effective. the three remaining vaccines--containing mineral-oil double ...19883202776
serological classification of australian and south african isolates of haemophilus paragallinarum. 19883214375
evaluation of the specificity and sensitivity of two commercial enzyme-linked immunosorbent assay kits, the serum plate agglutination test, and the hemagglutination-inhibition test for antibodies formed in response to mycoplasma gallisepticum.two commercial enzyme-linked immunosorbent assay (elisa) kits, seven serum plate agglutination (spa) antigens, and the hemagglutination-inhibition (hi) test for antibodies to mycoplasma gallisepticum (mg) were compared for sensitivity and specificity using known mg-positive and mg-negative sera from leghorn chickens. all spa antigens proved to be highly sensitive when testing mg-positive sera. laboratory-prepared spa antigens yielded fewer positive reactions when testing mg-negative sera than co ...19883041957
sensitivity and specificity of mycoplasma gallisepticum agglutination antigens prepared from medium with artificial liposomes substituting for serum.three batches of strain a5969 mycoplasma gallisepticum (mg) serum-plate-agglutination (spa) antigen grown in regular frey's medium with 12% swine serum, three batches grown in frey's medium containing artificial liposomes instead of serum, and one commercial spa antigen were evaluated for sensitivity and specificity. sensitivity was measured using chickens exposed to mg by intraocular and intranasal inoculation. specificity was measured in uninoculated controls and in groups inoculated with the ...19882461697
antimicrobial drug resistance and the occurrence of plasmids in haemophilus paragallinarum.a broth microdilution method was used to examine the sensitivity of 75 haemophilus paragallinarum isolates to six antimicrobial drugs (ampicillin, erythromycin, neomycin, penicillin, streptomycin, and tetracycline). a total of 55 of the isolates (73%) were sensitive to all six drugs. the remaining 20 isolates were resistant to streptomycin, with one of these isolates also being resistant to tetracycline and another also resistant to neomycin. no isolate showing antimicrobial drug resistance belo ...19883202771
[the antibacterial in vitro and in vivo effectiveness of enrofloxacin against haemophilus paragallinarum]. 19883219117
serological response of chickens either vaccinated or artificially infected with haemophilus paragallinarum.the serological response of chickens either vaccinated or artificially infected with haemophilus paragallinarum (hpg) serovar a or c was investigated using both a specific hemagglutinin (ha) antigen and a common ha antigen. with hpg serovar a, both vaccinated and artificially infected chickens produced hemagglutination-inhibition (hi) antibodies to hpg serovar-specific and hpg common ha antigens. most chickens vaccinated with hpg serovar c had detectable hi antibodies to both types of ha antigen ...19883401174
hemagglutinating activity and immunological properties of haemophilus paragallinarum field isolates in japan.four field isolates (s4, s10, s15, and s17) of haemophilus paragallinarum were recovered from chickens affected with infectious coryza in widely separated regions of japan. their hemagglutinating (ha) activity and immunological properties were compared with those of strain 221 of serovar a/1 and strains modesto and s1 of serovar c/2. when treated with potassium thiocyanate or hyaluronidase, all the isolates showed ha activity against formaldehyde-fixed chicken erythrocytes but not against fresh ...19892775098
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