Publications

further evidence for angiotensin-sodium interaction in central control of fluid balance.		1970	4320106
the distribution of smooth muscle in the cat ovary with a note on its adrenergic innervation.		1973	4722569
an environmental chamber system for prolonged metabolic studies on small animals.		1973	4771134
[histoenzymology of the heart conduction system in myocardial infarct in man].		1974	4431148
actinobacillus suis infection in pigs. a report of four outbreaks and two sporadic cases.		1974	4850396
actinobacillus suis in the horse.		1976	1265987
proceedings: uptake and incorporation of (u-14c)glucose during culture of mouse blastocysts previously undergoing normal implantation and induced delay of implantation.		1976	1255628
actinobacillus suis infections in alberta swine, 1973--75: pathology and bacteriology.		1976	974978
acute interstitial pneumonia [proceedings].		1977	193807
lysosomal acid hydrolases of normal adipose tissues of man, monkey and rat.		1978	97094
effect of spa waters from the hisar health resort springs "momina banja" and "momina salza" on experimentally induced gastric ulcer.		1978	104904
isolation of actinobacillus suis from a colt.		1978	630374
infection with actinobacillus suis in pigs.		1978	696543
interrupting the early region of polyoma virus dna enhances tumorigenicity.	the tumorigenicity of dna from polyoma virus after cleavage with a variety of restriction enzymes was evaluated in suckling hamsters. cleavage with enzymes that interrupt the region of the genome coding for the large tumor (t) antigen of polyoma virus markedly enhanced the tumorigenicity above that observed with dna i of the virus. cell lines established in vitro from tumors induced by polyoma virions, polyoma virus dna i, or polyoma virus dna that had been cleaved with restriction endonucleases ...	1979	226975
morphologic and functional characteristics of human intestinal lymphoid cells isolated by a mechanical technique.		1979	153255
morphological and functional aspects of the glomerular basement membrane.	addition of alcian blue to the fixative reveals structural elements bearing negatively charged sites within the glomerular capillary wall of the rat kidney. according to previous research (review by brenner et al., 1977), these charges represent one of the most important functional components of the glomerular filtration barrier. alcian blue also demonstrates negatively charged microfibrils (probably glycoproteins) of the mesangial matrix. preliminary observations of glomeruli of lower vertebrat ...	1979	95429
actinobacillus suis infection of horses.	nineteen isolates of actinobacillus suis were recovered from horses during the period october 1978-december 1980. animals varied in age from a full term foetus to 12 years. one isolate was obtained from the nose of an apparently healthy horse, the remainder were obtained from still-born foetuses (2), foals dying within a week of birth (5), older animals with respiratory (6) or genital infections (3) or abscesses in the jaw (1). one isolate was obtained from the lung of a 2-week-old foal which ha ...	1982	16030875
prevalence of organisms described as actinobacillus suis or haemolytic actinobacillus equuli in the oral cavity of horses. comparative investigations of strains obtained and porcine strains of a. suis sensu stricto.	evidence was obtained to indicate that equine strains of organisms previously described as actinobacillus suis or hemolytic variants of actinobacillus equuli might constitute a separate group of organisms provisionally designated taxon 11. four biovars were noticed within taxon 11. selected dna:dna hybridizations support the classification of the mannitol positive biovar 2 of taxon 11 distinct from porcine a. suis. the final taxonomical position of taxon 11, however, has to await more detailed g ...	1984	6532108
cefadroxil in the horse: pharmacokinetics and in vitro antibacterial activity.	sodium cefadroxil was administered as a single intravenous dose (25 mg/kg) to six healthy adult mares. plasma samples were collected over a 24-h period and cefadroxil concentrations were measured by microbiological assay. the pharmacokinetic behavior of the drug was appropriately described in terms of a one-compartment open model. values for the major pharmacokinetic terms were: extrapolated initial plasma concentration = 59.2 +/- 15.0 micrograms/ml; half-life = 46 +/- 20 min; apparent volume of ...	1985	4057345
[respiratory diseases of swine: some epidemiologic aspects].	epidemiological surveys enable to know better the aetiology of respiratory diseases. the surveys carried out in slaughter-houses in brittany (france) in 1980 and 1981, show a worrying situation concerning porcine respiratory diseases. microbiological studies brought out the preponderance of the isolation of mycoplasma hyopneumoniae, pasteurella multocida, bordetella bronchiseptica, streptococcus suis and actinobacillus suis. some patterns of experimental infection with mycoplasma hyopneumoniae a ...	1985	4064626
similarity principles and intrinsic geometries: contrasting approaches to interspecies scaling.	we criticize standard allometric approaches on the grounds that they emphasize scaling to one variable at a time, whereas chemically reactive hydrodynamic systems involved in pharmacokinetic phenomena are of higher dimension. we show that attempts based on mechanical similitude to set a dosage that would be equivalent across species (for example, from mouse to humans) lead to ambiguous results. another failing of standard allometry may be its incapability to accommodate the neoteny of homo sapie ...	1986	3820095
isolation of actinobacillus suis from a canada goose (branta canadensis).	blindness from conjunctivitis caused by actinobacillus suis was found in a canada goose (branta canadensis) from a wildlife refuge in kentucky. lesions were not observed elsewhere and other body organs were grossly normal. actinobacillus spp. in birds is rare and this is apparently the first report of conjunctivitis resulting from this infection in waterfowl.	1987	3625911
actinobacillus suis-like organisms in horses.	actinobacillus suis-like organisms have been recognized in equine specimens at the university of california veterinary medical teaching hospital since 1975. the most common source (65%) of the organism was transtracheal washings. the organism was gram-negative, produced hemolysis on blood agar, and gave a positive reaction for oxidase, urease, o-nitrophenyl-beta-d-galactopyranoside, and esculin. carbohydrate reactions were variable, consisting of 4 main patterns. actinobacillus suis-like organis ...	1987	3631683
qualitative and quantitative determination of enterobacterial common antigen (eca) with monoclonal antibodies: expression of eca by two actinobacillus species.	the presence and quantity of the enterobacterial common antigen (eca) in several species belonging to the family enterobacteriaceae as well as to other gram-negative families were determined by a solid-phase enzyme-linked immunosorbent assay system and western blotting by using mouse monoclonal antibodies specific for eca. except for erwinia chrysanthemi, previously known to be an exception, all species known or presumed to belong to enterobacteriaceae produced eca (89 of 90 species). most speci ...	1987	3818929
analysis of major antigens of haemophilus (actinobacillus) pleuropneumoniae and related organisms.	outer membrane protein (omp)-enriched extracts and whole-cell protein preparations of haemophilus (actinobacillus) pleuropneumoniae and related organisms were examined by polyacrylamide gel electrophoresis and immunoblotting. both the omp-enriched and whole-cell protein profiles of actinobacillus suis, a. pleuropneumoniae (nad-independent biovar), a. lignieresii, and pasteurella haemolytica were very similar to those of h. pleuropneumoniae serotypes 1 to 8. antisera prepared against h. pleuropne ...	1987	3298061
alteration of lung structure and function by pseudomonas aeruginosa.		1987	3122195
report of a case: removal of an antral dentigerous cyst via a modified caldwell-luc.		1987	265903
multiple cis-acting sequence elements are required for efficient splicing of simian virus 40 small-t antigen pre-mrna.	we have determined the effects of a number of mutations in the small-t antigen mrna intron on the alternative splicing pattern of the simian virus 40 early transcript. expansion of the distance separating the small-t pre-mrna lariat branch point and the shared large t-small t 3' splice site from 18 to 29 nucleotides (nt) resulted in a relative enhancement of small-t splicing in vivo. this finding, coupled with the observation that large-t pre-rna splicing in vitro was not affected by this expans ...	1988	2851720
evaluation of heat-sensitive, neutrophil-toxic, and hemolytic activity of haemophilus (actinobacillus) pleuropneumoniae.	cytotoxic and hemolytic activity of haemophilus (actinobacillus) pleuropneumoniae serotype 1 strain cm5 was investigated because of the potential role as a virulence determinant. viable bacteria were toxic for porcine and bovine neutrophils, whereas bacteria killed by heat treatment at 60 c for 1 hour were not. similarly, bacteria-free culture supernatant was cytotoxic and hemolytic in assays that used porcine neutrophils and erythrocytes, whereas supernatant treated at 60 c for 1 hour had no ac ...	1988	3421527
suppression of sv40-promoted gene expression by differentiation of preadipose cells.	when a plasmid bearing the chloramphenicol acetyltransferase (cat) gene under the control of an sv40 early promoter is introduced into preadipose or adipose cells of line 3t3-f442a, the promoter directs high levels of transient expression of cat. however, when the plasmid is introduced into preadipose cells and the cells are then allowed to differentiate into adipose cells, the expression of the cat gene is suppressed. in this process, the plasmid is not changed detectably in amount, topology, o ...	1988	2849579
ontario. actinobacillus suis septicemia mimicking erysipelas in sows.		1988	17423089
actinobacillus suis septicemia in mature swine: two outbreaks resembling erysipelas.	in the winter of 1987/88 a previously unrecognized septicemic disease syndrome - actinobacillosis in mature sows and gilts - was diagnosed in two minimal-disease swine herds in southwestern ontario. in herd 1, 34 sows, 2 boars, 13 feeder pigs, and 30 suckling pigs were affected; 11 sows, 2 feeders, and 18 suckling pigs died. in herd 2, 13 sows and 1 feeder pig were affected; 1 sow and 1 feeder pig died. the disease was manifested by moderate fever (39-40.5 degrees c), round or rhomboid erythemat ...	1989	17423473
immunoserological comparison of 104-kilodalton proteins associated with hemolysis and cytolysis in actinobacillus pleuropneumoniae, actinobacillus suis, pasteurella haemolytica, and escherichia coli.	a homologous polyclonal antibody was produced in a rabbit to the 104-kilodalton (kda) protein hemolysin of actinobacillus pleuropneumoniae serotype 1 strain cm-5. in immunoblots, this antibody recognized a similar 104-kda protein produced in culture supernatants by a. pleuropneumoniae serotypes 1 to 12 and taxon "minor group" in addition to pasteurella haemolytica, actinobacillus suis, and alpha-hemolysin-producing escherichia coli (but only weakly in the latter two organisms). these results wer ...	1989	2674017
opsonization of yeast by the plasma of biomphalaria glabrata (gastropoda): a strain-specific, time-dependent process.	phagocytosis of yeast by haemocytes of biomphalaria glabrata, intermediate host of schistosoma mansoni, is influenced strongly by host plasma components, although it can occur without involvement of such factors. plasma from two strains of b. glabrata which are resistant to s. mansoni differs in its opsonic properties from the plasma of a susceptible strain. this may reflect the principles which determine compatibility or incompatibility in this host-parasite system. opsonization is a time-depen ...	1989	2671868
malignant hyperpyrexia.		1989	2653537
middle ear disease and learning problems: a school system's approach to early detection.		1989	260791
[multiple admissions to the psychiatric hospital--a study of the status of so-called "revolving door patients"].	the graph of the frequency distribution of rehospitalization shows a constant exponential decline without demarking a particular population of so-called "revolving-door" patients. patients with at least four admissions to a regional psychiatry hospital within a time span of two years are compared to a control group of the total clinic population. multiple admission patients suffer significantly more often from diseases with an unfavourable prognosis, the illness has started earlier in their live ...	1990	1480673
synopsis of 1975 amendment to laws governing nursing in south carolina: extended and expanded roles of the nurse.		1990	267796
prevalence of seroreactors to the 104-kilodalton hemolysin of actinobacillus pleuropneumoniae in swine herds.	a rabbit homologous polyclonal antiserum to the 104-kilodalton hemolysin of actinobacillus pleuropneumoniae serotype 1 strain cm-5 was specifically produced and used in an antigen capture enzyme-linked immunosorbent assay (elisa) to detect swine serum antibodies to this potentially important virulence factor. sera from pigs experimentally infected with the most common disease-producing serotypes (serotypes 1, 2, 5, and 7) of a. pleuropneumoniae produced positive results in this elisa. of 144 ser ...	1990	2332472
in vitro susceptibility of selected veterinary bacterial pathogens to ciprofloxacin, enrofloxacin and norfloxacin.	the minimum inhibitory concentrations (mic) of ciprofloxacin, enrofloxacin, and norfloxacin were tested for approximately ten clinical isolates of each of actinobacillus pleuropneumoniae, actinobacillus suis, actinomyces pyogenes, corynebacterium pseudotuberculosis, erysipelothrix rhusiopathiae, haemophilus parasuis, haemophilus somnus, pasteurella haemolytica, pasteurella multocida, rhodococcus equi, streptococcus equi, streptococcus suis and streptococcus zooepidemicus. ciprofloxacin and enrof ...	1990	2306672
serodiagnosis of pleuropneumonia using enzyme-linked immunosorbent assay with capsular polysaccharide antigens of actinobacillus pleuropneumoniae serotypes 1, 2, 5 and 7.	capsular polysaccharide antigens of serotypes 1, 2, 5 and 7 of actinobacillus pleuropneumoniae were used in enzyme-linked immunosorbent assays (elisas) to test sera from experimentally infected and field pigs. specific reactions were found in sera of experimental pigs with antigens of serotypes 1, 5 and 7 whereas the serotype 2 antigen was cross-reactive. a 1:200 serum dilution was used for testing of 300 sera from 21 swine herds in southern ontario. cases of pleuropneumonia had occurred in 11 o ...	1990	2249177
very early aortic responses during atherosclerosis induction in rabbits: measurement by duplex ultrasound. i. non-invasive study of aortic hyperresponsiveness to serotonin.	we report the use of a non-invasive ultrasound method to visualize and measure changes in serotonin reactivity of the abdominal aorta during the early stages of atherosclerosis development. studies were performed at 0, 4, 8, and 14 weeks in new zealand white rabbits fed a diet enriched with 0.5% cholesterol. change in systolic vessel diameter at each examination was compared with changes in rabbits fed a control diet or rabbits on a diet enriched with cholesterol plus a concentrated marine lipid ...	1990	2202316
monoclonal antibody in the identification of haemophilus somnus.	electrophoretic comparisons of outer membrane proteins of haemophilus somnus isolates revealed 2 major protein bands (46 and 14 kilodaltons [kd]) common to all isolates tested. a monoclonal antibody raised against h. somnus reacted to the 46-kd band. coagglutination tests were performed using a monoclonal antibody coagglutination assay. the monoclonal reagent was produced by incubating cowan strain staphylococcus aureus suspension, used as a source of crude protein a, with mouse ascitic fluid mo ...	1990	2094432
actinobacillus suis infection in pigs in southwestern ontario.	actinobacillus suis was isolated from tissues of 39 pigs, 2 porcine lungs, and 1 uterine swab submitted for diagnostic evaluation from 24 farms in southwestern ontario between 1985 and 1988. these isolates represented a gradually increasing incidence of herd outbreaks caused by a. suis in southwestern ontario. the outbreaks were typified by sudden death in suckling or recently weaned pigs; 87% of the affected pigs examined at the laboratory were between two and 28 days old. petechial to ecchymot ...	1990	17423607
actinobacillus suis-like organisms and evidence of hemolytic strains of actinobacillus lignieresii in horses.	thirty-seven local isolates of actinobacillus suis-like organisms from diseased and clinically normal horses and 1 llama were compared with reference strains of a suis, a lignieresii, a equuli, a capsulatus, a hominis, a (pasteurella) ureae, and equine a suis-like organisms (aslo) previously described in literature. comparison was by cultural characteristics, carbohydrate fermentation, enzyme profiles, and whole-cell protein polyacrylamide gel electrophoresis. carbohydrate fermentation, determin ...	1991	1928905
immunological properties of actinobacillus pleuropneumoniae hemolysin i.	the 105 kda hemolysin i protein from actinobacillus pleuropneumoniae serotype i type strain 4074 (hlyi) was shown by immunoblot analysis to be the predominant immunogenic protein if convalescent field sera or sera from pigs experimentally infected with a. pleuropneumoniae serotype 1 were used. sds gel- and immunoblot-analysis using total culture, washed cells or culture supernatant showed that hlyi is essentially secreted and is not found attached to the bacteria. proteins in the 105 kda range t ...	1991	1887566
the cytotoxin of actinobacillus pleuropneumoniae (pleurotoxin) is distinct from the haemolysin and is associated with a 120 kda polypeptide.	mutants of actinobacillus pleuropneumoniae strain hk 361 (serotype 2) were isolated which were deficient in type ii (ca2(+)-dependent) haemolysin activity (hly-). some of the hly- mutants secreted a potent, heat-labile extracellular cytotoxic activity against porcine alveolar macrophages. comparison of cell-free culture supernatant from the parent strain and some hly- mutants by sds-page and immunoblotting revealed the loss of a major extracellular polypeptide of 109 kda. two hly- mutants which ...	1991	2033378
molecular characterization of an rtx toxin determinant from actinobacillus suis.	rtx cytolysins are a family of calcium-dependent, pore-forming, secreted toxins found in a variety of gram-negative bacteria. the prototypical member of this family is the alpha-hemolysin of escherichia coli. the rtx genetic determinants from seven members of the family pasteurellaceae, pasteurella haemolytica, actinobacillus actinomycetemcomitans, and a. pleuropneumoniae serotypes 1,5,7, and 9 were previously cloned and sequenced. using the leukotoxin determinant from p. haemolytica serotype a1 ...	1992	1587585
thermogenesis and mitochondrial gdp binding with age in response to the novel agonist cgp-12177a.	the ability to regulate body temperature diminishes with age in both humans and rodents. to investigate whether attenuation of sympathetically activated thermogenesis in brown adipose tissue (bat) may account for the loss of thermoregulation with age, we assessed o2 consumption and body temperature in response to norepinephrine and the specific bat beta-adrenergic agonist cgp-12177a in 6-, 18-, and 24-mo-old rats. in addition, the effects of this agonist on interscapular bat mitochondrial gdp bi ...	1992	1539643
[expression and distribution of catechol 2,3-dioxygenase in escherichia coli].	a series of new plasmids containing xyle gene was constructed based on the shuttle plasmid ptg 402 between e. coli and b. subtilis. the expressed xyle gene product catechol 2,3-dioxygenase (cato2ase) was measured for its output and distribution, and analysed for its structural hydrophobicity and hydrophilicity. it was demonstrated that the output of cato2ase was relative to plasmids, host cells, culture time and with or without induction. the enzyme didn't have features of excretional proteins a ...	1992	1524847
comparison of ascorbic acid and ascorbic acid 2-o-alpha-glucoside on the cytotoxicity and bioavailability to low density cultures of fibroblasts.	ascorbic acid 2-o-alpha-glucoside (aa-2g) is a stable ascorbate derivative which has vitamin c activity in vivo and in vitro. we studied whether aa-2g exerts a prooxidant action in cultured fibroblasts from chick embryo and human skin, as does ascorbic acid. at concentrations of 0.1-1.0 mm, ascorbic acid markedly reduced the viable cell number of low density cultures within 24 hr, whereas aa-2g had no such effect. the ascorbate cytotoxicity was dependent on the cell density at the time of its ad ...	1992	1472084
actinobacillosis in an alpaca (lama pacos).	the death of a 6-week-old alpaca being treated for acute polyarthritis is described. post-mortem and histological findings included fibrinous mural endocarditis, lung abscessation, acute passive congestion of the liver, embolic suppurative nephritis and chronic nonsuppurative polyarthritis. actinobacillus suis was isolated from the heart lesion and in pharyngeal swabs from four of six clinically normal alpacas in the same flock.	1992	16031654
quebec. actinobacillus suis in swine in southwestern quebec.		1993	17424312
evaluation of a saline boiled extract, capsular polysaccharides and long-chain lipopolysaccharides of actinobacillus pleuropneumoniae serotype 1 as antigens for the serodiagnosis of swine pleuropneumonia.	a saline boiled extract (sbe), capsular polysaccharides (cps) and long-chain lipopolysaccharides (lc-lps) of actinobacillus pleuropneumoniae serotype 1 have been evaluated in elisa for the serodiagnosis of swine pleuropneumonia caused by this serotype. mean optical densities (ods) obtained with the three antigens using sera from negative herds as well as from animals experimentally and naturally exposed to a. pleuropneumoniae serotypes 1, 9 or 11 were not significantly different. the positive el ...	1994	7886937
production of apx toxins by field strains of actinobacillus pleuropneumoniae and actinobacillus suis.	the three apx toxins of actinobacillus pleuropneumoniae have potential value for use in vaccines and diagnostic tests which will be species specific instead of serotype specific, provided that the apx toxins are species specific and all field strains produce these toxins. we examined 114 a. pleuropneumoniae field strains and found that they secreted either apxi, apxii, apxi and apxii, or apxii and apxiii and secreted no other cytolytic activities. however, proteins similar to apxi and apxii were ...	1994	8063425
simplified procedure for preparation of sensitized latex particles to detect capsular polysaccharides: application to typing and diagnosis of actinobacillus pleuropneumoniae.	a novel, inexpensive method for obtaining immunoglobulin g (igg) specific for capsular antigen is described for use in latex agglutination tests. hyperimmune rabbit serum against encapsulated actinobacillus pleuropneumoniae was thoroughly adsorbed with a nonencapsulated mutant. the capsule titer of the absorbed serum was unaffected, whereas reactivity to nonencapsulated cells was reduced to background levels, as determined by enzyme immunoassay. the igg component of the adsorbed serum was recove ...	1995	7494018
pasteurella multocida produces a protein with homology to the p6 outer membrane protein of haemophilus influenzae.	an antibody specific for a 16-kda outer membrane protein of a rabbit strain of pasteurella multocida was used to probe representatives of all 16 somatic serotypes of p. multocida, as well as the vaccine strains cu and m9, and all were shown to express the protein. the gene encoding this protein was cloned and sequenced and found to have extensive sequence homology with the gene encoding the p6 protein of haemophilus influenzae. the protein in p. multocida has been designated p6-like. the gene en ...	1995	7868272
[immunology of the placenta].		1995	173100
molecular characterization of a common 48-kilodalton outer membrane protein of actinobacillus pleuropneumoniae.	previous studies have shown that a vaccine prepared from outer membranes of actinobacillus pleuropneumoniae serotype 5 can elicit protective immunity in swine against challenge with either serotype 5 or serotype 1. these results suggest the presence of common subcapsular surface antigens, such as outer membrane proteins, that contribute to cross-protective immunity. we have identified a 48-kda outer membrane protein that is common to all 12 capsular serotypes of a. pleuropneumoniae but is not pr ...	1996	8557378
enhancement of host resistance to bacterial infections in normal and immunosuppressed mice with actinobacillus suis.	a single intraperitoneal (ip) inoculation of heat-killed actinobacillus suis atcc 15,557 (as 15,557) into normal and immunosuppressed (dexamethasone-treated) mice led to remarkable nonspecific resistance to ip challenge with lethal doses of opportunistic pathogens such as pseudomonas aeruginosa, methicillin-resistant staphylococcus aureus and candida albicans. the duration of this enhanced protective action and the minimal effective dose, in normal mice, induced by as 15,557 were superior to tho ...	1996	8741706
biochemical and antigenic relationships between porcine and equine isolates of actinobacillus suis.	a total of 50 actinobacillus suis isolates were studied for their biochemical and antigenic characteristics. of them, 40 isolates originated from different tissues of diseased pigs, and the other ten isolates were from horses with respiratory problems. there was no major biochemical difference among equine and porcine a. suis isolates. results of tube agglutination tests showed that porcines isolates were antigenically homogeneous while equine isolates were heterogeneous.	1996	8870199
actinobacillus suis septicaemia in two foals.	a 24-hour-old hackney ony filly developed signs of weakness, depression and a poor suck reflex, with harsh lung sounds over both fields, and a 48-hour-old arabian colt from a normal birth which had sucked vigorously developed loose stools and became depressed, weak and anorectic. both foals had serum igg concentrations greater than 800 mg/dl, but each had a severe neutropenia with a left shift, and blood cultures from both of them yielded actinobacillus suis. the a suis isolates had different an ...	1996	8629322
actinobacillus suis-like organisms associated with septicemia in neonatal calves.		1996	8744750
actinobacillus suis infection after a pig bite.		1996	8826821
serological and biochemical properties of the major outer membrane protein within strains of the genus actinobacillus.	sarcosyl-extracted outer membrane preparations of organisms of the genus actinobacillus were investigated with regard to heat-modifiable and serological properties as well as n-terminal amino acid sequencing of the isolated major outer membrane protein (omp). the major omp of actinobacillus lignieresii was recognized by a monoclonal antibody with specificity towards proteus mirabilis ompa. moreover, n-terminal amino acid sequencing revealed strong homology to ompa of enterobacteriaceae, on the c ...	1996	8837386
an outbreak of actinobacillus suis septicemia in grow/finish pigs.		1996	8844585
actinobacillus suis strains isolated from healthy and diseased swine are clonal and carry apxicabdvar. suis and apxiicavar. suis toxin genes.	actinobacillus suis isolates recovered from both healthy and diseased pigs were characterized by biochemical testing, serotyping, restriction endonuclease fingerprinting, and apx toxin gene typing. the clinical isolates analyzed were collected over a 10-year period from approximately 40 different locations in southwestern ontario, canada. little variation in the biochemical profiles of these isolates was seen, and all isolates reacted strongly with rabbit antisera prepared against one of the str ...	1997	9114394
acute hemorrhagic pulmonary infarction and necrotizing pneumonia in horses: 21 cases (1967-1993).	to characterize history, clinical signs, and pathologic findings in horses with histologically confirmed acute hemorrhagic pulmonary infarction and necrotizing pneumonia.	1997	9187729
synergistic effects of actinobacillus suis cells in combination with 5-fluorouracil on mice bearing murine l1210 leukemia cells.	the mean survival rates of female bdf1 mice transplanted intravenously (i.v.) with murine l1210 leukemia cells were significantly prolonged by intraperitoneal (i.p.) pretreatment (before i.v. transplantation) or by i.p. pre- and post-treatment (before and after the i.v. transplantation) with heat-killed actinobacillus suis cells atcc 15557 (as 15557) alone, as compared with untreated l1210-leukemia-cell-bearing control mice. however, significant prolongation of the mean survival rates was not el ...	1997	9222280
actinobacillus and streptococcus: producers of isoschizomers of the restriction endonucleases r.hphi, r.saui, r.nhei, r.mboi and r.swai.	new restriction endonucleases have been found in microorganisms isolated from the microflora of human teeth. the strain-producers are actinobacillus suis and streptococcus milleri. the new enzymes are isoschizomers of the prototypes as follows: asuhpi - hphi; asusai - saui; asunhi - nhei; asumbi and smimbi - mboi; smii - rare-cutter swai.	1998	9628357
pathogenesis and clinical signs of equine herpesvirus-1 in experimentally infected ponies in vivo.	equine herpesvirus-1 (ehv-1) causes respiratory disease, neonatal death, abortion and neurologic disease. the main purpose of this study was to identify viral antigen in respiratory tract samples by immunoperoxidase staining. six pony foals were selected on the basis of demonstrating seronegativity to ehv-1 by virus neutralization and housed in isolation. they were infected experimentally by administering ehv-1 nebulized ultrasonically through a face mask. successful infection was clinically app ...	1998	9442940
differentiation of actinobacillus pleuropneumoniae strains by sequence analysis of 16s rdna and ribosomal intergenic regions, and development of a species specific oligonucleotide for in situ detection.	the aims of this study were to characterize and determine intraspecies and interspecies relatedness of actinobacillus pleuropneumoniae to actinobacillus lignieresii and actinobacillus suis by sequence analysis of the ribosomal operon and to find a species-specific area for in situ detection of a. pleuropneumoniae. amplification and sequence analysis of the 16s-23s rdna ribosomal intergenic sequence (ris) from the three species showed the existence of two ris's, differing by about 100 bp. both se ...	1998	9779607
[clinical significance of lipid metabolic indices in diabetes mellitus in children].		1998	98917
traumatic shock monitored by doppler ultrasonic flowmeter. case report.		1998	124813
ocular adnexal problems in craniofacial deformities.		1998	108349
discussion: oxidative disorders.		1998	104556
electromyographic studies in acute period of trichinellosis.		1998	1189442
[ezymatic method for the automated determination of total serum cholesterol (author's transl)].	a simple kinetic method for the automated determination of total serum cholesterol was developed using cholesterol esterase, cholesterol oxidase, catalase and the color reaction according to hantzsch. the procedure has been adapted to the braun systematik, greiner gsa ii, eppendorf 5032, lars ljungberg autolab, perkin-elmer c4 and perkin-elmer c4b analyzers. the variants of the method yielded satisfactory results with regard of precision, accuracy and sensitivity to interferences.	1999	1189524
agents of the "suis-ide diseases" of swine: actinobacillus suis, haemophilus parasuis, and streptococcus suis.	in recent years, actinobacillus suis, haemophilus parasuis, and streptococcus suis have emerged as important pathogens of swine, particularly in high health status herds. their association with a wide range of serious clinical conditions and has given rise to the moniker "suis-ide diseases." these organisms are early colonizers and, for that reason, are difficult to control by management procedures such as segregated early weaning. vaccination, serodiagnostic testing, and even serotyping are com ...	1999	10369563
isolation of actinobacillus suis from a cat's lung.	actinobacillus suis has been isolated from the lungs of 9-month-old cat. the bacterium was characterized biochemically as well as genetically, and its sensitivity profile to different antimicrobial agents was established. the role of this isolate in the cat's condition is discussed.	1999	9919368
comparative pathogenicity of different actinobacillus suis o/k serotypes.	the pathogenicity of actinobacillus suis serotypes o1/k1 (strain so4), o1/k2 (strain c84), and o2/k2 (strain h91-0380) was evaluated in specific-pathogen-free (spf) piglets challenged by intraperitoneal inoculation with approximately 1 x 10(7) colony-forming units per ml. all 3 strains produced peritonitis, but differences were observed in the composite histopathologic scores (p = 0.001) and in their ability to spread (p = 0.008) at 7 h post challenge. the o2/k2 strain caused the most severe per ...	2000	10805245
apx toxins in pasteurellaceae species from animals.	pasteurellaceae species particularly of porcine origin which are closely related to actinobacillus pleuropneumoniae were analyzed for the presence of analogues to the major a. pleuropneumoniae rtx toxin genes, apxicabd, apxiica and apxiiicabd and for their expression. actinobacillus suis contains both apxicabd(var.suis) and apxiica(var. suis) operons and was shown to produce apxi and apxii toxin. actinobacillus rossii contained the operons apxiica(var.rossii) and apxiiicabd(var.rossii). however, ...	2000	10831858
prevalence of o1/k1- and o2/k3-reactive actinobacillus suis in healthy and diseased swine.	a cell surface antigen-typing system was devised for the swine pathogen actinobacillus suis and used to examine the prevalence of different lipopolysaccharide (o) types in healthy and diseased pigs. the strains examined in this study were isolated from a variety of locations in canada and from kansas. lipopolysaccharide preparations of 151 isolates of a. suis were characterized by immunoblotting using polyclonal antisera generated to strains so4 (o1/k1), h89-1173 (o2/k3), and vsb 3714, a rough s ...	2000	11015398
a 24-kda cloned zinc metalloprotease from actinobacillus pleuropneumoniae is common to all serotypes and cleaves actin in vitro.	actinobacillus pleuropneumoniae causes pleuropneumonia in swine. this bacterium secretes proteases that degrade porcine hemoglobin and iga in vitro. to further characterize a. pleuropneumoniae proteases, we constructed a genomic library expressed in escherichia coli dh5alpha, and selected a clone that showed proteolytic activity. the recombinant plasmid carries an 800-base pair a. pleuropneumoniae gene sequence that.codes for a 24-kda polypeptide. a 350-base pair psti fragment from the sequence ...	2000	10805246
actinobacillus suis infection in pigs in australia.	this is the first report of the isolation of actinobacillus suis in association with significant disease among preweaned pigs in australia. sudden deaths occurred in preweaned piglets at one facility and enlarged joints, particularly the stifles and tarsi, occurred at another. isolates with the biochemical phenotype and apx genotype profile of a suis were cultured from affected piglets. both facilities were of high health status and one had undergone a recent depopulation, disinfection and sow r ...	2000	10904814
detection and subtyping of actinobacillus pleuropneumoniae strains by pcr-rflp analysis of the tbpa and tbpb genes.	a pcr-based procedure for detection and serotype identification of actinobacillus pleuropneumoniae strains was developed and evaluated. the a. pleuropneumoniae tbpa and tbpb genes were used as targets for amplification of dna fragments, with a pair of specific primers for each gene. amplification with tbpa primers rendered a 2.8-kb pcr product from all 12 a. pleuropneumoniae reference strains as well as from actinobacillus suis strain ccm 5586, while amplification of a 1.9-kb pcr product was obs ...	2000	11081581
the first description of a (1--> 6)-beta-d-glucan in prokaryotes: (1 --> 6)-beta-d-glucan is a common component of actinobacillus suis and is the basis for a serotyping system.	the chemical and antigenic properties of the cell-surface lipopolysaccharides (lpss) and capsular polysaccharides (cpss) of seven representative strains of actinobacillus suis from healthy and diseased pigs were investigated. four strains produced a linear (1 --> 6)-beta-d-glucan homopolymer, beta-d-glcp-(1-[ --> 6)-beta-d-glcp-(1-]n -->, as a lps-o-chain (o1) and as a cps (k1). polyclonal antisera prepared against a (1 --> 6)-beta-d-glucan-containing strain showed a positive reaction against bo ...	2000	11086692
in vitro susceptibility of porcine respiratory pathogens to tilmicosin.	bacterial isolates obtained from swine with various clinical diseases were tested for susceptibility to tilmicosin by minimum inhibitory concentration (mic) and kirby-bauer disk diffusion tests using national committee on clinical laboratory standards methodology. the tilmicosin mic90 was < or =0.125 microg/ml for erysiopelothrix rhusiopathiae, < or = 1 microg/ml for haemophilus parasuis isolates, 8 microg/ml for actinobacillus suis and pasteurella multocida type a, 16 microg/ml for toxigenic an ...	2000	11108454
developmental-behavioral dysfunction in preschool children. descriptive analysis of a pediatric consultative model.	a study of 79 preschool children referred to an interdisciplinary clinic because of behavioral problems and developmental concerns was done. historical data and information regarding present functioning were collected using standard questionnaires. in addition to medical, neurological, and psychiatric examinations, every child received a neurodevelopmental assessment. when compared with a matched cohort of children from the community, the clinic population had a higher incidence of developmental ...	2000	92192
the relationship between venous pressure and volume in the lower extremity during exercise.		2000	1189589
the effect of the sclerosing agent "hydroxypolyaetoxydodecan" on the coagulation potentials: in vitro investigations.		2000	1189590
editorial independence of prescribers' journal.		2001	87757
in-vitro evidence for adenoma-carcinoma sequence in large bowel.		2001	87924
the rubella immunity gap.		2001	87852
umbilical-cord insertion as source of pure fetal blood for prenatal diagnosis.		2001	87711
serologic profile of a cohort of pigs and antibody response to an autogenous vaccine for actinobacillus suis.	actinobacillus suis is a commensal opportunistic pathogen in swine. however, in recent years, an increasing prevalence of clinical signs associated with a. suis has been observed in high health status herds in north america. the objectives of the study were to assess the kinetics of antibodies to a. suis in pigs from a herd showing clinical signs of a. suis infection and, to evaluate the antibody response in gilts following vaccination with an autogenous vaccine. an enzyme-linked immunosorbent a ...	2001	11361153
the transferrin receptor of actinobacillus pleuropneumoniae: quantitation of expression and structural characterization using a peptide-specific monoclonal antibody.	when actinobacillus pleuropneumoniae (a. pp) is grown under iron-restricted conditions in vitro, transferrin binding proteins (tbps) are induced. the functional transferrin receptor of a. pp is composed of two outer membrane proteins (tbp1 and tbp2) and shows an exquisite specificity for porcine transferrin. this complex was studied using a monoclonal antibody (mab 1.48) raised against a synthetic peptide corresponding to a hydrophilic domain of tbp2 common to several a. pp serotypes. the antibo ...	2001	11356318
reclassification of equine isolates previously reported as actinobacillus equuli, variants of a. equuli, actinobacillus suis or bisgaard taxon 11 and proposal of a. equuli subsp. equuli subsp. nov. and a. equuli subsp. haemolyticus subsp. nov.	members of bisgaard taxon 11 have been isolated from horses. these bacteria are of importance in the veterinary clinic and also to the medical profession, since they may be isolated from infected wounds of humans bitten by horses. six strains from different continents were identified as taxon 11, with 16s rrna similarities between 98.0 and 99.7%. a single isolate that represented the so-called (+)l-arabinose-positive actinobacillus equuli isolated from a diseased foal showed 99.9% 16s rrna simil ...	2002	12361259
analysis of non-porcine isolates of actinobacillus suis.	twenty-four actinobacillus suis isolates obtained from several species of non-porcine mammals were compared to the representative porcine strains, atcc 15557 (serotype o1) and h89-1173 (serotype o2), by o serotyping, dna fingerprinting, pcr amplification of apxica, apxiica and apxiiica toxin genes and by rrs (16s rrna) gene sequencing. only two strains, both equine, reacted with o1 antiserum while two others, one canine and the other feline, reacted with o2 antiserum. one equine strain reacted w ...	2002	11792495
tiamulin activity against fastidious and nonfastidious veterinary and human bacterial isolates: initial development of in vitro susceptibility test methods.	tiamulin is a pleuromutilin derivative used in veterinary practice for the control and specific therapy of infections in swine. this report summarizes studies to establish standardized susceptibility testing methods, interpretive criteria, and reagent details for use in veterinary methods recently developed by the national committee for clinical laboratory standards (nccls) (standards m31-a and m37-a, nccls, wayne, pa., 1999). a total of 636 fastidious and nonfastidious animal and human pathogen ...	2002	11825957