Publications

TitleAbstractYear
Filter
PMID
Filter
[actinobacillus actinomycetemcomitans causing subacute endocarditis]. 19892490473
immunodominant antigen of actinobacillus actinomycetemcomitans y4 in high-responder patients.this study was undertaken to look for characteristics of the immunodominant antigen(s) of actinobacillus actinomycetemcomitans y4 that might help explain the high antibody titers in periodontitis patients. radioimmunoassays (ria) were performed on sera from 481 patients; sera from the 32 patients with the highest anti-y4 titers (above 128,000 ria u/ml) were further analyzed. y4 antigen was boiled for 45 min or treated with papain, and antibody responses were analyzed by ria and western blotting ...19892496034
biochemical and serological properties of actinobacillus pleuropneumoniae biotype 2 strains isolated from swine.the biochemical and serological properties of 21 strains of actinobacillus pleuropneumoniae biotype 2 isolated from haemorrhagic necrotic pleuropneumonia of swine were examined. for serologic typing, the indirect haemagglutination (iha) and the double gel-diffusion tests were used. on the basis of their soluble surface antigens, our a. pleuropneumoniae biotype 2 isolates could be assigned to two proposed serotypes. serotype 1 comprised 11 strains and serotype 2 comprised 10 strains. all strains ...19892505435
effect of bicarbonate on the growth of actinobacillus actinomycetemcomitans in anaerobic fructose-limited chemostat culture.the effect of bicarbonate on the growth and product formation by a periodontopathic bacterium, actinobacillus actinomycetemcomitans, was examined in an anaerobic chemostat culture with fructose as the limiting nutrient. the chemostat cultures were run at dilution rates between 0.04 and 0.25 h-1 and the maximum growth yield (ymax fructose) was estimated to be 40.3 and 61.7 g dry wt (mol fructose)-1 in the absence and presence of bicarbonate, respectively. the major fermentation products in the ab ...19892517636
failure of bacteroides gingivalis w83 to accumulate bound c3 following opsonization with serum.our previous studies have demonstrated that strains of bacteroides gingivalis are capable of proteolytic degradation and inactivation of complement proteins including the third component of complement c3. since a crucial step in the ability of complement to control bacterial infections is the binding of c3 fragments to the bacterial surface with subsequent enhancement of phagocytosis, further examination of the importance of the proteolytic capacity of bacteroides in interactions with complement ...19892524566
bactericidal concentrations of chlorhexidine-digluconate, amine fluoride gel and stannous fluoride gel for subgingival bacteria tested in serum at short contact times.in vitro inhibitory and bactericidal concentrations in serum of chlorhexidine-digluconate, amine fluoride gel, stannous fluoride gel, stannous fluoride, metronidazole and amoxicillin were determined against bacteroides gingivalis, bacteroides intermedius, fusobacterium nucleatum, actinobacillus actinomycetemcomitans and capnocytophaga sputigena. the minimal inhibitory concentration was assessed by the agar dilution technique. the killing curves and minimal bactericidal concentration of the antim ...19892524581
lps-elicited secretory responses in monocytes: altered release of pge2 but not il-1 beta in patients with adult periodontitis.lipopolysaccharide responsiveness in human subjects was assessed through the examination of lps-stimulated pge2 and il-1 beta release from counterflow isolated monocytes from patients with varying levels of periodontal destruction. this study was performed in order to investigate a possible relationship between lps-mediated secretory responses in monocytes and susceptibility to periodontal destruction in humans. subjects were chosen based on apparent resistance or susceptibility to disease as me ...19892524582
serotype-related differences in production and type of heat-labile hemolysin and heat-labile cytotoxin of actinobacillus (haemophilus) pleuropneumoniae.reference strains of serotypes 1 to 12 of actinobacillus (haemophilus) pleuropneumoniae were cultured in eagle minimal essential medium with 10% serum plus. culture supernatants were examined for cytotoxicity to alveolar macrophages and for the ability to hemolyze sheep erythrocytes. all strains except the reference strain of serotype 6 produced cytotoxin, whereas only serotypes 1, 5, 9, 10, and 11 produced hemolysin. both cytotoxin and hemolysin appeared to be heat labile. antisera were raised ...19892526820
inhibitory effects of rabbit antisera on mitogenic activity of actinobacillus actinomycetemcomitans lipopolysaccharide.lipopolysaccharides (lpss) were isolated from actinobacillus actinomycetemcomitans strains atcc 29523 (serotype a), y4 (b), and nctc 9710 (c) by the hot phenol-water procedure. y4 lipid a was obtained by the hydrolysis of y4 lps in 1% acetic acid. all the lps preparations and y4 lipid a were mitogenic for c3h/hen mouse spleen cells, but not for c3h/hej mouse spleen cells. immunoglobulin preparations partially purified by ammonium sulfate precipitation at 33% saturation from rabbit antisera again ...19892528615
[the influence of previous infections on the development of experimental swine pleuropneumonia in specific pathogen free pigs].this study was designed to determine in six-week old specific pathogen free pigs, the effect of previous experimental exposure to mycoplasma hyopneumoniae and transmissible gastroenteritis virus on a challenge infection with actinobacillus pleuropneumoniae. pigs exposed simultaneously to m. hyopneumoniae and transmissible gastroenteritis virus appeared more resistant to challenge (one week later) with a. pleuropneumoniae. four pigs out of a group of ten died following the challenge infection, co ...19892531628
comparison of conventional and long-acting oxytetracyclines in prevention of induced actinobacillus (haemophilus) pleuropneumoniae infection of growing swine.these experiments tested the hypothesis that long-acting oxytetracycline (oxytetracycline-la) was more effective than regular oxytetracycline in preventing porcine pleuropneumonia when administered either 24 or 48 h prior to experimental challenge with virulent strains of actinobacillus pleuropneumoniae. two experiments (1 and 2) were conducted using growing pigs (average weight 12-15 kg). antibiotic treatments were administered once intramuscularly at 20 mg/kg body weight; controls received an ...19892531629
[a latex agglutination test for the rapid identification of periodontally pathogenic microorganisms in subgingival plaque. the preliminary results].latex agglutination tests (la) have been evaluated for identifying periodontal pathogens. this preliminary study compared la tests with indirect immunofluorescence (if) for the rapid identification of a. actinomycetemcomitans, b. gingivalis, and b. intermedius in clinical subgingival plaque samples. a total of 58 sites from 12 patients suffering from advanced periodontitis, exhibiting recent attachment loss were examined before and after initial periodontal treatment. the results of the two exam ...19892532826
endobronchial inoculation of various doses of haemophilus (actinobacillus) pleuropneumoniae in pigs.twelve-week-old specific-pathogen-free pigs were inoculated deep in the bronchi with haemophilus (actinobacillus) pleuropneumoniae strain 13261 in doses ranging from 8 x 10(1) to 9 x 10(7) colony-forming units (cfu). pigs that survived infection were euthanatized and examined 48 hours after inoculation. the relationship between dose and severity of disease was evaluated clinically and the weight of pneumonic lesions was compared. the relationship between infection dose and weight of pneumonic le ...19892532869
[gene cloning of an actinobacillus actinomycetemcomitans y4 antigen that reacts to peripheral blood sera in advanced destructive periodontitis patients].actinobacillus actinomycetemcomitans (a.a.) has been implicated in the etiology of juvenile periodontitis and also of advanced destructive periodontitis (adp). it has been reported that the levels of the igg antibody against the a.a. in the peripheral blood sera of the adp patients were often high as well as those against the bacteroides gingivalis. to clone the genes of antigen reactive to the sera of the adp patients, we constructed a phage library of the a.a. strain y4 dna in lambda l47, whic ...19892533606
[new prophylactic guidelines for infectious endocarditis in patients with early periodontal disease].it is necessary to add to the antibiotic prophylaxis suggestions of the infectious endocarditis facing odontologic treatment that, in the patient to treat, clinic forms of jpl and rpp are rejected because of their proved relation with the actinobacillus actinomycetemcomitans and because this last one is resistant to the recommended prophylaxis methods.19892534684
characterization of a streptomycin-sulfonamide resistance plasmid from actinobacillus pleuropneumoniae.an actinobacillus pleuropneumoniae strain contained a plasmid (phd8.1) conferring resistance to streptomycin and sulfonamide. restriction endonuclease mapping and dna-dna hybridization showed that phd8.1 is related to rsf1010 from salmonella panama, which also confers resistance to streptomycin and sulfonamide, and to phd148 from haemophilus ducreyi, which confers resistance only to sulfonamide.19892541656
development of a shuttle vector and a conjugative transfer system for actinobacillus pleuropneumoniae.an original genetic system for actinobacillus pleuropneumoniae has been developed. a shuttle cloning vector, pyg53, was constructed from the wild-type plasmid pyg10. it permits, in conjunction with electroporation, the introduction of cloned genes into this species. a conjugal transfer system between escherichia coli and a. pleuropneumoniae involving pyg54, a mobilizable derivative of pyg53, is also described. conjugation efficiencies of 8.3 x 10(-3) exconjugants per donor can be obtained.19892559875
enzyme-linked immunosorbent assay and immunoblot analysis of the immunoglobulin g response to whole-cell and lipooligosaccharide antigens of pasteurella pneumotropica in laboratory mice with latent pasteurellosis.the immunoglobulin g (igg) response to whole-cell and lipooligosaccharide (los) antigens of pasteurella pneumotropica was evaluated in mice with latent pasteurellosis by enzyme-linked immunosorbent assay (elisa) and immunoblots. antibodies to cell wall proteins of p. pneumotropica also reacted with several protein antigens from isolates of actinobacillus spp. and other pasteurellae. conversely, antibodies to los antigens of p. pneumotropica demonstrated no cross-reactivity with loss of other pas ...19892584371
serum levels of antibodies against actinobacillus actinomycetemcomitans in various forms of human periodontitis.serum levels of igg, iga, and igm antibodies against extracts from bacteroides gingivalis per8, actinobacillus actinomycetemcomitans y4, and bacteroides fragilis nctc 9343 were determined in three categories of periodontitis patients by means of enzyme-linked immunosorbent assay. the test groups comprised 10 patients with juvenile periodontitis (jp), 18 young patients with severe periodontitis (yp), and 31 patients with adult periodontitis (ap). nine subjects with healthy periodontium (hp) serve ...19892589029
dna probe detection of key periodontal pathogens in juveniles.recognition of juvenile forms of periodontitis have been shown to be directly linked with specific gram-negative rods, primarily actinobacillus actinomycetemcomitans, bacteroides gingivalis and bacteroides intermedius. however, clinical application of these laboratory findings have generally been restricted to the research environment. the purpose of this study was to explore the relationship of these three pathogenic species in children using the reliable and accurate new technology of dna prob ...19892593067
activation of human natural killer cells by lipopolysaccharide from actinobacillus actinomycetemcomitans.lipopolysaccharide (lps) from the y4 strain of this bacterium, which is implicated in the pathogenesis of juvenile periodontitis, was incubated with human peripheral blood lymphocytes (pbl) and its action compared to that of lps from escherichia coli. both lps augmented cytotoxicity measured against natural killer (nk) cell-resistant tumour targets within 24 h of incubation. cytotoxicity was exclusively found in nk-enriched low-density large granular lymphocyte fractions, as separated by percoll ...19892597038
antigens of actinobacillus actinomycetemcomitans identified by immunoblotting with sera from patients with localized human juvenile periodontitis and generalized severe periodontitis.sonicated whole cell extracts and outer membrane proteins from this bacterium were analysed using sera from 31 young patients with localized juvenile periodontitis, 55 young adults with generalized severe periodontitis and from 31 healthy control subjects. the sonicate contained 13 major bands (14-78 kda); a greater proportion of sera from patients with generalized periodontitis reacted with 40 and 70 kda antigens when compared with sera from localized juvenile periodontitis and controls. in con ...19892597055
prevalence of periodontal breakdown in adolescents and presence of actinobacillus actinomycetemcomitans in subjects with attachment loss.the present investigation was carried out to study the prevalence and clinical appearance of destructive periodontal disease in a school population of 15- to 16-year old adolescents in amsterdam. in addition the prevalence of actinobacillus actinomycetemcomitans was studied in those subjects showing attachment loss. attachment loss was diagnosed in 230 of the 4565 subjects participating which is about 5% of the population studied. within this population males were more frequently affected than f ...19892600747
clinical and microbiological characteristics of treated periodontitis patients on maintenance care.a population of 98 adults previously treated for moderate to advanced adult periodontitis and currently maintained in a recall program for at least one year were recruited for this study. the ultimate purpose of the study is to determine whether the presence of actinobacillus actinomycetemcomitans, bacteroides gingivalis, and bacteroides intermedius in selected periodontal sites can predict the future clinical course of these patients, particularly with respect to disease recurrence. this report ...19892600753
isolation of actinobacillus (haemophilus) pleuropneumoniae from pneumonic lungs of slaughtered pigs. 19892601240
actinobacillus actinomycetemcomitans infection. 19892602770
infection due to actinobacillus actinomycetemcomitans. 19892602771
availability of laboratory testing services for identification of periodontal pathogens in dental plaque.microbiologic monitoring of key subgingival plaque organisms has been proposed to enhance management of some patients with destructive forms of periodontal disease. however, isolation and identification of many of these species is available only through selected microbiology laboratories possessing special expertise with periodontal microorganisms. the purpose of this study was to determine the availability of periodontal microbiology laboratory services in the u.s. and canada that identify susp ...19892605862
an in vitro method to study the adherence of oral bacteria to hela cells.exfoliated buccal epithelial cells have been widely used in microbial adherence studies, but present a number of problems due to their variable nature and to contamination with indigenous bacteria. an adherence assay was developed using hela cell monolayers which were washed with buffer, or treated with saliva or serum to mimic buccal or crevicular epithelial cells, respectively. a total of eighteen strains of oral bacteria tested showed a low affinity for untreated hela cells, but most strains ...19892607993
[the synergism of actinobacillus pleuropneumoniae and influenza a virus in experimentally-infected mice].models for infecting mice with influenza a-virus (a/pr 8/34, h0n1) and actinobacillus pleuropneumoniae (serotype 9) were developed in han: nmri-mice. after infecting mice with sublethal doses of one of the infectious agents, or both together as a mixed infection, animals were subsequently exsanguinated and the lungs washed by bronchoalveolar lavage. clinical symptoms were recorded daily, examination of lung lavage fluid and sera as well as histology of the lungs were done. an increase in mortali ...19892609806
cytologic and bacteriologic evaluation of tracheobronchial aspirates from clinically normal foals.thirty-eight tracheobronchial aspirates (tba) were collected from twenty 1 to 6-month-old foals, which were free of clinical signs of respiratory tract or other infectious disease. we collected tba from 9 of the foals 3 times when they were approximately 8, 16, and 24 weeks old. aspirates were examined cytologically after staining with modified wright-giemsa, gram, toluidine blue, and prussian blue stains. aerobic bacterial culturing was performed on all aspirates. of the 20 initial tba, 4 (20%) ...19892610430
in vitro susceptibilities of oral bacterial isolates to spiramycin.four hundred strains of oral bacteria were tested for their susceptibility to spiramycin. actinobacillus actinomycetemcomitans and most species of lactobacillus were resistant to the antibiotic. all strains of cariogenic streptococcus mutans and most strains of bacterial species implicated in adult chronic periodontitis (bacteroides gingivalis, b. intermedius, and treponema denticola) were susceptible to spiramycin.19892610512
transformation of actinobacillus pleuropneumoniae and analysis of r factors by electroporation.an efficient method for dna transfer is essential for the genetic manipulation of any organism. such a capacity will be required for the genetic analysis of actinobacillus pleuropneumoniae as a swine pathogen, as well as for its manipulation for vaccination purposes. for this reason, the use of electroporation as a means of plasmid dna introduction into this species was examined. the multiple antibiotic-resistant strain 80-8141 of actinobacillus pleuropneumoniae harbors 3 plasmids: pyg10, pyg15, ...19892619125
comparison of bacteriologic culture of blood and necropsy specimens for determining the cause of foal septicemia: 47 cases (1978-1987)diagnosis of bacterial septicemia was confirmed by results of bacteriologic culture of antemortem blood samples and/or necropsy specimens obtained from 47 foals up to 8 days old. gram-negative bacteria were isolated from all 47 foals, and mixed infections with more than one organism were involved in 26 (55%). escherichia coli, actinobacillus spp, and klebsiella pneumoniae were the most frequent isolates, infecting 55, 34, and 23% of foals, respectively. gram-positive bacteria and anaerobic bacte ...19892624660
in vivo chemotaxis evoked by actinobacillus actinomycetemcomitans and haemophilus aphrophilus.the chemotaxis-evoking capacity of 5 actinobacillus actinomycetemcomitans and 5 haemophilus aphrophilus strains were studied in a tissue cage model in rabbits. a significant increase of the total number of polymorphonuclear leukocytes was induced in the tissue cage fluid by both viable and killed bacteria, reaching a maximum after 12-24 h. in parallel, the proportion of viable/non viable leukocytes increased. the leukocyte counts declined during the following 24-48 h in all chambers except in th ...19892628865
intra-oral colonization of macaque monkeys by actinobacillus actinomycetemcomitans.actinobacillus actinomycetemcomitans was acquired by captive macaca fascicularis 3 to 6 months after birth, and all monkeys aged over 6 months harbored detectable levels. this microorganism was most frequently isolated from the gingival plaque of the incisor (and other) teeth compared with other oral sites. strains were leukotoxic by bioassay and western blot analysis. antibodies in macaque serum contained neutralized the leukotoxin of a human a. actinomycetemcomitans strain. high titres of mate ...19892628866
experimental infections by bacteroides gingivalis in non-immunized and immunized rabbits.the interactions between bacteroides gingivalis and systemic antibodies were studied in tissue cages implanted in the backs of new zealand white rabbits. infectivity was evaluated according to clinical signs and to leukocyte and bacterial counts in material aspirated from the tissue cages. pre- and post-inoculation antibody levels to sonicated whole bacterial cells were determined by enzyme-linked immunosorbent and agar immunodiffusion assays. rabbits immunized against b. gingivalis and then cha ...19892628870
[microbiological diagnosis of microorganisms tentatively designated as "sp organisms"].the authors describe the biochemical characteristics of two strains described as "sp organism". this microorganism incertae sedis resembles from the biochemical aspect (oxidase+, mannite-, dextrose+ with gas) the species pasteurella aerogenes; contrary to the latter it does not break down urea and differs also as regards the morphology of colonies, which on blood agar are coarser; it also has a higher content of g + c in dna than pasteurella. it is a pathogen of rodents. the authors describe two ...19892632012
[characteristics of certain early periodontitis: apropos of two cases].the study of early onset periodontitis led in the last years to important improvement in the etiopathogenic knowledge of periodontitis. the clinical and microbiological analysis of 2 early onset periodontitis cases gives a better understanding of the evolution of these 2 models of periodontal lesions. hypothesis are presented concerning the transition between localized juvenile periodontitis and rapidly advanced periodontitis.19892632773
antimicrobial action of natural substances on oral bacteria.the antimicrobial action of natural substances was investigated in vitro against oral bacteria including streptococcus sp., actinomyces sp., actinobacillus sp., bacteroides sp., capnocytophaga sp., eikenella sp., fusobacterium sp. and propionibacterium sp. among the natural substances tested, hinokitiol was the most inhibitory to oral bacteria. cinnamon bark oil, papua-mace extracts, and clove bud oil in spice extracts were also inhibitory against many oral bacteria. egg white lysozyme exhibited ...19892637783
[effects of anti-bacteroides gingivalis (bg), and anti-actinobacillus actinomycetemcomitans (aa) antibodies on bg and aa].the purpose of this study was to determine the effects of anti-bacteroides gingivalis (bg) and anti-actinobacillus actinomycetemcomitans (aa) antibodies on bg and aa respectively. bg and aa were resistant to complement-mediated bactericidal activity, and killing by pmn. however a significant reduction in cfu of bg and aa was found when these bacteria were incubated with the antibodies and not agitated. this would suggest that the antibody was capable of aggregating or phagocytizing, but not kill ...19892637905
[antibiotic therapy of bacterial infections of the oral cavity].more than 300 commencial bacterial species may be found in the oral cavity. other microorganisms, such as mycoplasms, mycetes, protozoa and viruses are present as well. the virulency of the saprofites and additional contamination by outside microorganisms are factors determining the development of infectious process in the oral tissues. moreover, streptococci and anaerobes are the most frequent aetiology agents. the antibiotic therapy should comply with the general treatment criteria, on the one ...19892638255
[microbiological changes in subgingival flora after treatment with amoxicillin-clavulanic acid].the association amoxicillin-clavulanic acid can be employed as an alternative to the usual antibiotic therapy of periodontitis. the purpose of this study was to determine subgingival microbial changes in 33 patients with periodontitis after using amoxicillin (500 mgrs.-t.i.d.) and clavulanic acid (125 mgrs.-t.i.d.) for 5 days. it resulted clinically in a decrease of both gingival index and plaque index (not significant) and microbiologically in absence of the main bacterial pathogens found pretr ...19892638817
effects of periodontopathic bacteria on il-1 and il-1 inhibitor production by human polymorphonuclear neutrophils.the effect of heat-killed periodontopathic bacteria on the production of interleukin-1 (il-1) and an il-1 inhibitor by human polymorphonuclear neutrophils (pmn's) was examined. peripheral blood was obtained from 18 healthy volunteers and the pmn's were separated using dextran sedimentation and ficoll-paque density gradient centrifugation. the pmn's (5 x 10(5) cells/well) were cultured in serum-free media with or without heat-killed periodontopathic bacteria. four gram-negative periodontopathic b ...19892640313
plasmids in actinobacillus actinomycetemcomitans strains isolated from periodontal lesions of patients with rapidly destructive periodontitis.several strains of actinobacillus actinomycetemcomitans newly isolated from periodontal lesions of patients with rapidly destructive periodontitis were all shown to possess identical plasmid profiles consisting of 4 plasmids. the largest plasmid, 20 megadalton (mda), was also found in reference strains. two different methods were used for isolation of the plasmids; the large 20 mda plasmid (phrp1) was found using the kado and liu method only. the 3 small plasmids of 7.0, 5.2 and 4.0 mda (phrp2, ...19892640316
the occurrence of actinobacillus actinomycetemcomitans, bacteroides gingivalis, bacteroides intermedius and spirochaetes in the subgingival microflora in relation to the early onset of periodontitis in a group of adolescents.subgingival samples were obtained from mesial and distal surfaces of first molars in 100 subjects aged 14 years at baseline. sites were monitored at 6-monthly intervals for clinical loss of attachment. six subjects showed greater than 1 mm loss of attachment at one site 18-24 months later. further samples were obtained from these sites together with a comparison sample from the contralateral site and equivalent sites in a matched control subject. actinobacillus actinomycetemcomitans was more fre ...19892640319
clinical, microbiological and immunological studies of post-juvenile periodontitis.the present study includes the clinical, microbiological and immunological examinations of 2 patients with post-juvenile periodontitis. bacteroides intermedius was the predominant isolate from periodontal pockets with post-localized juvenile periodontitis. bacteroides gingivalis, bacteroides forsythus and actinobacillus actinomycetemcomitans were detected in samples from periodontal pockets with post-generalized juvenile periodontitis. igg antibody levels to b. gingivalis were significantly high ...19892640920
infection due to actinobacillus actinomycetemcomitans: 15 cases and review.actinobacillus actinomycetemcomitans is a small fastidious gram-negative coccobacillus that fails to grow on macconkey's agar. slow growth in broth may lead to delays in diagnosis. first described in 1912, a. actinomycetemcomitans has been recognized since 1962 as capable of causing serious infections in humans. such infections include periodontal infection, soft tissue abscess (often in association with actinomyces species), and systemic infection, most commonly endocarditis. fifteen cases and ...19892644690
identification and expression of the actinobacillus actinomycetemcomitans leukotoxin gene.the leukotoxin produced by the oral bacterium actinobacillus actinomycetemcomitans has been implicated in the pathogenesis of juvenile periodontitis. in order to elucidate the structure of the leukotoxin, molecular cloning of the leukotoxin gene was carried out. a dna library of a. actinomycetemcomitans, strain jp2, was constructed by partial digestion of genomic dna with sau3ai and ligation of 0.5 to 5.0 kilobase pair fragments into the bam hi site of the plasmid vector penn-vrf. after transfor ...19892647082
black-pigmented bacteroides species and actinobacillus actinomycetemcomitans in subgingival plaque of adult kenyans.a microbiological study was performed of the subgingival plaque on 2 sites in each of 20 adults originating from a rural area 40 km outside nairobi, kenya. the recovery rate of b. gingivalis was 70%, of b. intermedius 100% and of a. actinomycetemcomitans 40% of the subjects, and 50%, 90% and 28%, respectively, of the sites. the isolated strains exhibited similar biochemical characteristics and antibiotic susceptibility pattern as type strains of these species. the high recovery rate of these 3 b ...19892656770
haemophilus pleuropneumoniae in pigs: a review.haemophilus (actinobacillus) pleuropneumoniae is a primary and specific pathogen of the respiratory tract and is an economically important pathogen of pigs. the disease is starting to cause peracute deaths in south africa and the chronic form leads to deteriorating heard performance. this review highlights various aspects of the taxonomic, antigenic, and drug sensitivity characteristics of the bacterium and the epidemiology, clinical signs, pathology, serology and immunology, detection and diagn ...19892657061
the role of antibody, complement and neutrophils in host defense against actinobacillus actinomycetemcomitans.a. actinomycetemcomitans is a facultative gram-negative coccobacillus which has been implicated in the etiology and pathogenesis of localized juvenile periodontitis and has also been recognized for its potential to cause serious extraoral infections, particularly endocarditis. the polymorphonuclear neutrophil has been suggested to play a key role in host resistance to periodontopathic organisms, as indicated by the association between defective production or function of these phagocytic cells an ...19892659510
cloning and expression of a cohemolysin, the camp factor of actinobacillus pleuropneumoniae.the genetic determinant of the cohemolysin which is responsible for the camp phenomenon, a cohemolysis, of actinobacillus pleuropneumoniae was cloned in escherichia coli. total dna from the a. pleuropneumoniae serotype 1 type strain 4074 was used to construct a gene library in plasmid puc18 in e. coli jm83. a total of 10,500 clones containing recombinant plasmids have been screened for hemolysis on blood plates. fifty-five clones which showed a weak hemolytic response after 24 to 48 h of incubat ...19892659534
analysis of the actinobacillus actinomycetemcomitans leukotoxin gene. delineation of unique features and comparison to homologous toxins.actinobacillus actinomycetemcomitans leukotoxin has been implicated as a virulence factor in human infections. to initiate delineation of leukotoxin structure/function relationships, molecular cloning of the leukotoxin gene was carried out. when an a. actinomycetemcomitans genomic dna library in lambda embl3 was screened using a 1.3-kilobase pair restriction fragment containing a portion of the leukotoxin gene, 13 positive recombinants were identified. one recombinant, designated lambda op8, con ...19892670940
immunoserological comparison of 104-kilodalton proteins associated with hemolysis and cytolysis in actinobacillus pleuropneumoniae, actinobacillus suis, pasteurella haemolytica, and escherichia coli.a homologous polyclonal antibody was produced in a rabbit to the 104-kilodalton (kda) protein hemolysin of actinobacillus pleuropneumoniae serotype 1 strain cm-5. in immunoblots, this antibody recognized a similar 104-kda protein produced in culture supernatants by a. pleuropneumoniae serotypes 1 to 12 and taxon "minor group" in addition to pasteurella haemolytica, actinobacillus suis, and alpha-hemolysin-producing escherichia coli (but only weakly in the latter two organisms). these results wer ...19892674017
actinobacillus actinomycetemcomitans serotypes and leukotoxicity in korean localized juvenile periodontitis.actinobacillus actinomycetemcomitans is though to play an important role in the pathogenesis of localized juvenile periodontitis (ljp). preliminary data suggested that the serotype distribution of a. actinomycetemcomitans in korea and the united states differ. this study evaluated a. actinomycetemcomitans prevalence, serotype distribution, and leukotoxicity in korean ljp patients by culture, enzyme-linked immunosorbent assay, indirect immunofluorescence, and lactate dehydrogenase release from po ...19892677302
effect of 0.12% chlorhexidine on bacterial recolonization following periodontal surgery.bacterial plaque accumulation following periodontal surgery has been directly associated with delayed and altered surgical wound healing. successful antimicrobial treatment following periodontal surgery depends upon the elimination and suppression of wound associated microorganisms. highly effective antimicrobials should also prevent recolonization of periodontopathogens. in this investigation, the antimicrobial effect of a 0.12% chlorhexidine gluconate mouthrinse (peridex) on bacterial recoloni ...19892681676
characterization of a multiple antibiotic resistance plasmid from haemophilus ducreyi.plasmid pls88 from a clinical isolate of haemophilus ducreyi encoded resistance determinants for sulfonamides and streptomycin related to those of rsf1010 and for kanamycin related to tn903 but lacked the inverted repeats of the transposon. its host range included haemophilus influenzae, actinobacillus pleuropneumoniae, and escherichia coli; and it was compatible with pdm2 and rsf1010.19892684012
[bacterial invasion in periodontitis, is it important in periodontal treatment?].during the progression of periodontal disease bacteria invade the epithelial lining of the gingival pocket and the underlying connective tissue. this invasion was demonstrated in acute necrotizing ulcerative gingivitis (anug), localized juvenile periodontitis (ljp), rapidly progressive periodontitis in adults (rpp) and in children with papillon-lefèvre syndrome associated periodontitis. using morphologic criteria or fluorescent antibody staining techniques spirochetes, actinobacillus actinomycet ...19892685948
gas chromatographic assessment of alcoholyzed fatty acids from yeasts: a new chemotaxonomic method.an alternative chemotaxonomic method to methanolysis was developed for gas chromatographic assessment of fatty acids in whole yeast cells. clinical and reference strains of the medically important yeasts candida albicans, torulopsis glabrata, and saccharomyces cerevisiae were cultured for 48 h at 26 degrees c. cellular lysis and transesterification were then performed with ethanol, propanol, butanol, or methanol. the relative recovery rates for cellular fatty acids, including the volatile acids ...19892687322
biology of haemophilus ducreyi.the etiological agent of the sexually transmitted genital ulcer disease chancroid was first described in 1889 by auguste ducrey following repeated autoinoculation of purulent ulcer material from a series of patients. the organism was isolated on artificial media a decade later but has remained difficult to isolate consistently, resulting in controversy over its characteristics and role as the causative agent of chancroid. because of its fastidious growth requirements, including unknown component ...19892687678
[actinobacillus ureae meningitis. apropos of a case].a case of actinobacillus ureae meningitis in a 52 year old man with an history of chronic sinusitis and previous skull fractures is reported. actinobacillus ureae, called for a long time pasteurella ureae, is a rare cause of meningitis as shown by a short review of the literature (eight cases reported). we have searched the bacteria in the rhinopharynx of the patient's four dogs: this search was unsuccessful in agreement with the literature data which has not yet established an animal host for a ...19892691966
[isolation and identification of pasteurellaceae (haemophilus, pasteurella, actinobacillus and related taxa)]. 19892692587
cloning and characterization of a hemolysin gene from actinobacillus (haemophilus) pleuropneumoniae.neutralizing antisera to the leukotoxin secreted by pasteurella haemolytica neutralized the hemolysin of actinobacillus pleuropneumoniae and recognized a 110-kd antigen in cell-free culture supernatants from this organism. a series of nine overlapping recombinant phage clones carrying the gene for this 110-kd antigen were identified using affinity-purified anti-hemolysin antibody and a dna probe containing sequences from the p. haemolytica lktca genes. eight of the nine clones expressed a 110-kd ...19892693022
the microbiology of hiv-associated periodontal lesions.2 intraoral lesions associated with human immunodeficiency virus (hiv) infection have recently been described: an atypical gingivitis and a rapidly progressive periodontitis. the microbiota associated with these gingival and periodontal lesions was investigated. subgingival plaque samples were taken from 45 hiv-seropositive homosexual men and from 44 hiv-seronegative control subjects. each sampled site was clinically and radiographically classified as hiv-associated gingivitis, hiv-associated pe ...19892693496
[microbiological and serological investigation of periodontal disease activity].if disease activity in periodontal disease can be determined, the therapeutic measures of periodontitis may become diverse and different from patient to patient. the purpose of the present study was to evaluate the usefulness of microbiological and serological examination of periodontopathic bacteria in detecting destructive periodontal disease activity. one hundred and forty-eight sites in 52 subjects with moderate to severe periodontitis were studied clinically. clinical parameters included pl ...19892700196
[microbiological evaluation of initial preparation for adult periodontitis patients].the microbial flora from 46 adult periodontitis lesions of 23 patients and 18 sites in 9 healthy persons were examined and levels of serum igg antibody to gram negative periodontal disease-associated bacteria were measured by enzyme-linked immunosorbent assay. plaque samples and serum samples were taken 40-50 days after initial preparation consisting of scaling and root planing. to evaluate the effects of the therapy on 10 patients with adult periodontitis, changes in clinical parameters were co ...19892700354
[microbial flora of periodontitis and monitoring of the effect of initial preparation by immunofluorescence microscopy].the effect of initial preparation in adult periodontitis was evaluated by changes in clinical parameters and immunofluorescence microscopic counts of periodontal disease associated bacteria. subgingival plaque samples were taken with sterilized paper points from 10 sites of 5 periodontally healthy persons and 44 sites of 23 adult periodontitis patients. twenty-one diseased sites were periodically examined after plaque control and scaling, root planing. the direct immunofluorescence technique was ...19892700361
[reduction of a. actinomycetemcomitans in the subgingival microflora of adult advanced periodontitis].in 15 adult patients with severely advanced periodontal disease unrelated to localized juvenile periodontitis high numbers of a. actinomycetemcomitans (a. a.) were identified in samples of subgingival plaque (median log cfu 5.13). in order to correlate clinical conditions with bacteriological findings, in every patient 2 deep periodontal pockets were treated with a 3-phase regimen of minocycline administration, mechanical, and surgical periodontal therapy. a. a. was eliminated by minocycline alo ...19892702168
subgingival microflora, dental and periodontal conditions in patients with hereditary fructose intolerance.hereditary fructose intolerance (hfi) is a rare autosomally recessive disease which leads to severe hypoglycemia. the fructose-free diet of these patients apparently influences dental health. half of the patients are free of caries, but there are no reports on their periodontal condition nor on the composition of their subgingival microflora. therefore 18 patient with hfi were examined for the following parameters: radiographic bone loss, caries rate, gingival bleeding, occurrence of calculus, p ...19892704977
cloning and expression of the leukotoxin gene from actinobacillus actinomycetemcomitans.the leukotoxin produced by actinobacillus actinomycetemcomitans has been implicated in the etiology of juvenile periodontitis. to initiate a genetic analysis of the role of this protein in disease, we have cloned the leukotoxin gene in escherichia coli. recombinant colonies carrying toxin gene sequences were isolated by screening a genomic a. actinomycetemcomitans library with a dna probe for the leukotoxin gene from a related bacterium, pasteurella haemolytica. to demonstrate that the cloned a. ...19892707855
outer membranous vesicles and leukotoxic activity of actinobacillus actinomycetemcomitans from subjects with different periodontal status.strains of a. actinomycetemcomitans (a.a) from juvenile periodontitis patients (jp), adult periodontitis patients (ap), and 14-yr-old healthy children were tested for the correlation between leukotoxin activity and the number of outer membranous vesicles measured in electron micrographs. to determine the potential for connective tissue destruction following the interaction of polymorphonuclear leukocytes (pmn) with the bacteria, the lysosomal release of neutrophil elastase was assessed. the high ...19892711122
identification of the heat-labile hemolysin of actinobacillus pleuropneumoniae serotype 1.the heat-labile hemolysin of actinobacillus pleuropneumoniae serotype 1 strain cm-5 was partially purified using ammonium sulfate precipitation and gel permeation chromatography. this partially purified material was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and blotted onto nylon filters. the filters were treated with convalescent pig serum and subsequently with cm-5 culture supernatant containing active hemolysin. a 104 kd peptide was identified as the hemolysin bec ...19892713790
immunohistochemical localization of immunoglobulins in bovine granulomatous lesions.the immunohistochemical distribution of igg, iga and igm in granulomatous lesions caused by actinomyces bovis, actinobacillus lignieresi, actinomyces (corynebacterium) pyogenes, pseudomonas aeruginosa, staphylococcus aureus and mycobacterium bovis was studied. numerous igg-containing cells (plasma cells) were distributed in the peripheral connective tissue layers, but not in the epithelioid cell layer. a few scattered iga- and igm-containing cells were found in all the lesions examined. mycobact ...19892715395
selected low-cohesion variants of actinobacillus actinomycetemcomitans and haemophilus aphrophilus lack distinct antigens recognized by human antibodies.actinobacillus actinomycetemcomitans omz 346 a and haemophilus aphrophilus omz 384 a, isolated on a synthetic selective and differentiating agar, show the highly cohesive and wall adherent growth in liquid medium which is typical for all primary oral isolates of these species. from each of them a low cohesion variant, omz 346 f and omz 384 f, respectively, was obtained by selection for cells growing in suspension. screening of western blots of these four strains with several human sera revealed ...19892719526
periodontal condition and microbiology of healthy and diseased periodontal pockets in type 1 diabetes mellitus patients.on the basis of glycosylated hemoglobin (hba1c) values, 22 type 1 (insulin-dependent) diabetic adults were grouped into patients with near normal (hba1c less than or equal to 7.7%) and poor (hba1c greater than or equal to 9.9%) metabolic control. a total of 44 subgingival sites were examined for actinobacillus actinomycetemcomitans, black-pigmented bacteroides species and capnocytophaga species. no significant difference could be demonstrated between patients in the 2 test groups with regard to ...19892723104
differentiation of some species of neisseriaceae and other bacterial groups by dna-dna hybridization.dna-dna hybridization using total genomic dna probes may represent a way of differentiating between miscellaneous bacterial species. this was studied with type and reference strains of 20 species in moraxella, kingella, and other selected gram-negative groups. both radioactive and biotin labelling were employed. most of the species examined were easily distinguished, such as moraxella (branhamella) catarrhalis, m.(b.) ovis, m. atlantae, m. phenylpyruvica, m. osloensis, neisseria elongata, n. men ...19892730785
antigens released from four oral bacteria in periodontitis.antigens fractionated from cultures of four oral bacteria were tested for binding of serum igg, iga and igm from patients in early and established phase of periodontitis. bacteroides gingivalis and a.actinomycetemcomitans released antigens that discriminated between serum from individuals with or without periodontitis. the discriminating antigens ranged from 10 to 43 kda and included neutral sugar and protein but no lipids. significantly increased levels of igg and iga antibodies to the antigens ...19892731969
[serologic studies on the presence of antibodies against haemophilus (actinobacillus) pleuropneumoniae at swine breeding farms in north brabant].serological screening for the presence of antibodies to both h. pleuropneumoniae serotype 2 and serotype 9 using the complement fixation test was carried out on twenty-nine piglets production farms of an integrated group in the province of north brabant. antibodies to h. pleuropneumoniae serotype 2 and serotype 9 were not detected on six of the twenty-nine farms when a single screening was performed. the proportions of farms with animals serologically positive in regard to serotypes 2 and 9 were ...19892734766
endocarditis from a dental focus. importance of oral hygiene in valvar heart disease.fastidiously growing bacteria more and more are recognised as a source of infectious endocarditis. over recent years, three new cases of endocarditis caused by actinobacillus actinomycetemcomitans were diagnosed in our institution. the rise in frequency is possibly secondary to better laboratory skills. two patients with actinobacillus (haemophilus) actinomycetemcomitans endocarditis presented the classical history of preexisting valvar disease together with poor dental hygiene. the third patien ...19892737778
antibiotic susceptibility of serotype 2 and 5 strains of actinobacillus (haemophilus) pleuropneumoniae isolated from swine from 1974 to 1986.antibiotic susceptibility of 129 isolates of actinobacillus (haemophilus) pleuropneumoniae was examined. all the strains were isolated in 1974 to 1986 from the nasal cavities of swine housed on 35 farms in 11 prefectures of japan. all 28 strains of serotype 2 isolated before 1984 were susceptible to 10 antibiotics used. in contrast, more than one-half of 79 strains of serotype 2 isolated in 1985 and 1986 were resistant to aminoglycosides, tetracycline, tylosin and carbadox, and most of them show ...19892739211
antimicrobial susceptibility of actinobacillus (haemophilus) pleuropneumoniae isolated from pigs with pleuropneumonia. 19892739226
genetic homogeneity of actinobacillus seminis isolates.isolates of actinobacillus seminis from clinical cases and reference sources had markedly similar bam h1 restriction endonuclease profiles but were readily distinguishable from the bam h1 profiles of the histophilus-haemophilus group as well as from a lignieresii. for epidemiological purposes this lack of interstrain variation in bam h1 profiles makes restriction endonuclease analysis of isolates of a seminis unsuitable.19892740636
actinobacillus (haemophilus) actinomycetemcomitans: resistance to mercuric chloride of 41 strains isolated in brazil. 19892748992
[the "diseased" or "dead" guillemots (uria aalge), three-toed gulls (rissa tridactyla), silver gulls (larus argentatus) and laughing gulls (larus ridibundus) found in the area of the german bay, 1982-1985].between 1982 and 1985 the cadavers of 50 guillemots (uria aalge), 41 kittiwakes (rissa tridactyla), 26 herring gulls (larus argentatus) and 34 black-headed gulls (larus ridibundus) were examined pathological, bacteriological and virological. the probable cause of death was established. parasitosis were particularly prevalent in herring gulls (49%), where the main infection--as in black-headed gulls--was with cestoides. in kittiwakes and guillemots mainly spiruroideae were recorded. the commonest ...19892752934
pasteurella haemolytica-like bacterium from a progressive granuloma of cattle in brazil.three strains of a pasteurella haemolytica-like bacterium were isolated from lesions of a progressive granuloma of cattle in southern brazil. their characteristics and their differentiation from p. haemolytica varieties and actinobacillus lignieresii are described. the name "pasteurella granulomatis" is proposed for this apparently new taxon.19892754010
actinobacillus actinomycetemcomitans contamination of toothbrushes from patients harbouring the organism.the main ecological niche of actinobacillus actinomycetemcomitans (a.a.) seems to be the periodontal pocket, but it can also be isolated from supragingival plaque, buccal and tongue mucosa, or saliva. we examined toothbrushes from 21 patients, all identified as harbouring moderate to large numbers of a.a. in subgingival plaque, for contamination with this organism. 29% of the toothbrushes presented by our patients yielded detectable numbers of a.a. immediately after toothbrushing this figure ros ...19892760252
activity of amine-stannous fluoride combination and chlorhexidine against some aerobic and anaerobic oral bacteria.the in vitro susceptibility of 128 bacterial strains was tested to amine fluoride-stannous fluoride (amf + snf) and chlorhexidine (chx) solutions. actinobacillus actinomycetemcomitans, streptococcus mutans, and bacteroides intermedius were among the species investigated. the 50% and 90% minimal inhibitory concentrations (mics) were assessed by an agar dilution method. the mic ranges for a. actinomycetemcomitans were 2-32 micrograms/ml for chx and 0.25-64 micrograms/ml for amf + snf. the respecti ...19892762015
opsonic igg antibody against actinobacillus actinomycetemcomitans in localized juvenile periodontitis.actinobacillus actinomycetemcomitans is a gram-negative bacterium frequently recovered from periodontal lesions of patients with localized juvenile periodontitis (ljp). elevated levels of serum igg and igm antibodies to a. actinomycetemcomitans antigens are frequently observed in ljp patients, although the functional properties of such antibodies have not been characterized systematically. in this study, we analyzed serum from ljp subjects infected with a. actinomycetemcomitans with respect to t ...19892762021
the role of actinobacillus actinomycetemcomitans in periodontal disease.the different forms of periodontitis are all associated with a distinct bacterial flora. however, a clear relationship is described between localized juvenile periodontitis (ljp) and actinobacillus actinomycetemcomitans. eleven ljp patients all harboured a. actinomycetemcomitans. the arrest of ljp is often impossible, due to the inability to eliminate a. actinomycetemcomitans. therefore, antibiotic treatment was introduced in combination with the initial treatment. we studied the effect of perio ...19892767772
prosthetic valve endocarditis and paravalvular abscess caused by actinobacillus actinomycetemcomitans. 19892772472
[direct detection of actinobacillus (haemophilus) pleuropneumoniae in the lungs of swine].by means of cultural examination, coagglutination test (ct) and indirect fluorescent-antibody-technique (ifat) a total of 199 lung specimens from necropsy pigs from northwestern germany with symptoms of pleuropneumonia was examined for actinobacillus (haemophilus) pleuropneumoniae (ap). the ct was used to detect type specific antigens in lung extracts and the ifat was performed on tissue sections. both tests were found to be specific. detection and identification of ap by either test were succes ...19892776673
chemical differences in lipopolysaccharides from actinobacillus (haemophilus) actinomycetemcomitans and haemophilus aphrophilus: clues to differences in periodontopathogenic potential and taxonomic distinction.while actinobacillus actinomycetemcomitans has been associated with rapidly progressive periodontal destruction in man, the closely related haemophilus aphrophilus has not been related to periodontal disease. this may be due to differences in composition and structure of the lipopolysaccharides (lps) of these dental-plaque bacteria, since lps probably exerts a series of detrimental effects on the periodontium. lps was prepared by the phenol-water procedure from the type strains of a. actinomycet ...19892777374
coaggregation of fusobacterium nucleatum, selenomonas flueggei, selenomonas infelix, selenomonas noxia, and selenomonas sputigena with strains from 11 genera of oral bacteria.twenty-eight strains of fusobacterium nucleatum and 41 selenomonas strains, including s. sputigena (24 strains), s. flueggei (10 strains), s. infelix (5 strains), and s. noxia (2 strains), were tested for their ability to coaggregate with each other and with 49 other strains of oral bacteria representing actinobacillus, actinomyces, bacteroides, capnocytophaga, gemella, peptostreptococcus, porphyromonas, propionibacterium, rothia, streptococcus, and veillonella species. selenomonads coaggregated ...19892777378
actinobacillus prosthetic valve endocarditis. 19892801491
molecular cloning and characterization of a hemolysin gene from actinobacillus (haemophilus) pleuropneumoniae.this article describes the molecular cloning and expression of a hemolysin gene from a serotype 1 strain of actinobacillus pleuropneumoniae. the hemolysin was a thermolabile protein with an apparent molecular weight of 29,500 (29.5k hemolysin). unlike expression of the recently described 105k hemolysin of a. pleuropneumoniae (j. frey and j. nicolet, fems microbiol. lett. 55:41-46, 1988), expression of this hemolysin was not regulated by ca2+. antiserum prepared against the 105k hemolysin did not ...19892807529
structures and sugar compositions of lipopolysaccharides isolated from seven actinobacillus pleuropneumoniae serotypes.highly purified lipopolysaccharide (lps) preparations obtained from seven actinobacillus pleuropneumoniae strains representative of seven different serotypes were used to determine the structure and monosaccharide composition of the polysaccharide components of each lipopolysaccharide. an indication of the structure of each lps was obtained by procedures that included sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by silver staining and gel chromatographic fractionation of ac ...19892807553
characterization of some previously unclassified pasteurellaceae isolated from hamsters.bacteria isolated from purulent processes on the jaws of european hamsters (cricetus cricetus) and from intestinal inflammatory processes in syrian hamsters (mesocricetus auratus), bred as laboratory animals have been shown to be phenotypically similar but not identical with pasteurella pneumotropica. deoxyribonucleic acid (dna)-dna hybridization studies indicate that with one exception, the strains represent two new species of the family pasteurellaceae. in the absence of a close genomic relate ...19892808184
clinical and laboratory characterization of early onset periodontitis.clinical and laboratory data were compared in 72 patients with localized periodontitis (lp) and 103 patients with generalized periodontitis (gp). significantly more lp than gp cases had decreased neutrophil chemotaxis (ctx), and were seropositive for actinobacillus actinomycetemcomitans (aa). significantly, more gp cases were seropositive for bacteroides gingivalis (bg). all clinical indices were similar on affected teeth in lp and gp, but the attachment loss was greater on clinically unaffected ...19892810010
[study of the possibility of breeding serologically negative animals from a breeding farm infected with serotype 2 actinobacillus pleuropneumoniae].two sanitation trials showed that it was possible to rear serologically negative animals from pigs on a breeding farm infected with actinobacillus pleuropneumoniae serotype 2. this was achieved by the medication of sows and piglets during the farrowing-house period and by isolating the piglets from the infected stock of pigs immediately after weaning. medication of the sows consisted in administering sulphamethoxazole-trimethoprim in the drinking water for a period from five days before to eight ...19892815050
Displaying items 601 - 700 of 4767