| function of two novel single-crd containing c-type lectins in innate immunity from eriocheir sinensis. | c-type lectin is one of the pattern-recognition proteins of the non-self-innate immune system in invertebrates. in this study, two novel c-type lectin cdnas (esctl1 and esctl2) of eriocheir sinensis were cloned and characterized. esctl1 has 169 amino acids, whereas esctl2 has 164 amino acids. these two lectins contain one carbohydrate-recognition domain. phylogenetic analysis showed that esctl1 and esctl2 were not clustered with other reported lectins from crabs. esctl1 and esctl2 were expressed ... | 2014 | 24561128 |
| an ldla domain-containing c-type lectin is involved in the innate immunity of eriocheir sinensis. | c-type lectins (ctls) have crucial functions in recognizing and eliminating pathogens in innate immunity. this study identified a novel low-density lipoprotein receptor class a (ldla) domain-containing ctl, designated as esctldcp, from the chinese mitten crab eriocheir sinensis. the esctldcp cdna is 1258 bp long, with a 975 bp open reading frame that encodes a 324-amino acid protein. esctldcp contains a signal peptide, an ldla, and a single c-type lectin-like domain. esctldcp was only expressed ... | 2014 | 24140432 |
| some observations on the physiology of pseudomonas natriegens nov. spec. | | 1961 | 13733692 |
| dissimilation of glucose and gluconic acid by pseudomonas natriegens. | eagon, r. g. (university of georgia, athens) and c. h. wang. dissimilation of glucose and gluconic acid by pseudomonas natriegens. j. bacteriol. 83:879-886. 1962-when glucose dissimilation of a marine pseudomonad, pseudomonas natriegens, was studied, enzymes of both the glycolytic pathway and of the hexose monophosphate pathway were detected in extracts of glucose-grown cells. enzymes of the entner-doudoroff pathway and phosphoketolase were not detected. data from radiorespirometric experiments ... | 1962 | 13888944 |
| pseudomonas natriegens, a marine bacterium with a generation time of less than 10 minutes. | eagon, r. g. (university of georgia, athens). pseudomonas natriegens, a marine bacterium with a generation time of less than 10 minutes. j. bacteriol. 83:736-737. 1962.-pseudomonas natriegens, a marine microorganism, was demonstrated to have a generation time of 9.8 min. this is the shortest generation time reported to date. optimal growth occurred at 37 c in brain heart infusion broth supplemented with 1.5% sea salt. | 1962 | 13888946 |
| multiple forms of phosphofructokinase in animals from the phylum chordata. | | 2002 | 134863 |
| inspiring nursing students to mobilize communities in nepal. | in 1987 nurse educators in nepal implemented a revised certificate nursing curriculum oriented toward primary health care (phc) and a bachelor of community health nursing (chn) curriculum with emphasis on leadership in phc. as both curricula included practical experience for nursing students in the community, the teachers had to strategize how to maximize both the students' learning and their contribution to community health. below, how the educators encouraged reluctant students to not only par ... | 2008 | 2249925 |
| dice: nonclinical causes of overtreatment. | | 2010 | 2916481 |
| marine bacterioplankton can increase evaporation and gas transfer bymetabolizing insoluble surfactants from the air-seawater interface. | hydrophobic surfactants at the air-sea interface can retard evaporative and gaseous exchange between the atmosphere and the ocean.while numerous studies have examined the metabolic role of bacterioneuston at the air-sea interface, the interactions between hydrophobic surfactants and bacterioplankton are not well constrained. a novel experimental design was developed, using vibrio natriegens and (3)h-labelled hexadecanoic acid tracer, to determine how the bacterial metabolism of fatty acids affec ... | 2009 | 19431239 |
| 2-methoxy-2',4'-dichloro chalcone as an antimicrofoulant against marine bacterial biofilm. | marine paint mixed with 2-methoxy-2',4'-dichloro chalcone is able to considerably reduce the formation of biofilm by vibrio natriegens, a marine bacterium, on polycarbonate (pc), polymethylmethacrylate (pmma) and glass fiber reinforced plastic (gfrp). these polymers have been selected for the study, since they have wide marine applications. surfaces coated with dichloro chalcone containing marine paint had the lowest number of colony forming units (cfu) (1-5×10(6)), proteins (20-30 μg/cm2) and c ... | 2010 | 20708908 |
| identification of vibrio natriegens uvra and uvrb genes and analysis of gene regulation using transcriptional reporter plasmids. | nucleotide excision repair (ner) rectifies a variety of chemically and structurally distinct dna lesions. the current model of ner is based upon the enteric bacterium escherichia coli and there is scarce information about how other bacterial species respond to, and correct, dna damage. here we report the isolation and functional analysis of the uvra and uvrb genes from vibrio natriegens, a naturally occurring marine bacterium. genetic studies were completed to assess the repair capabilities of v ... | 2010 | 21046343 |
| [genotypic analysis and plant growth-promoting ability of four plant growth-promoting bacteria from mangrove]. | we identified four strains of plant growth-promoting bacteria (pgpb) and their plant growth-promoting ability. | 2010 | 21141471 |
| relationship between ion requirements for respiration and membrane transport in a marine bacterium. | intact cells of the marine bacterium alteromonas haloplanktis 214 oxidized nadh, added to the suspending medium, by a process which was stimulated by na+ or li+ but not k+. toluene-treated cells oxidized nadh at three times the rate of untreated cells by a mechanism activated by na+ but not by li+ or k+. in the latter reaction, k+ spared the requirement for na+. intact cells of a. haloplanktis oxidized ethanol by a mechanism stimulated by either na+ or li+. the uptake of alpha-aminoisobutyric ac ... | 1984 | 6690427 |
| Production and characterization of a novel monoclonal antibody against Vibrio parahaemolyticus F(0)F(1) ATP synthase's delta subunit and its application for rapid identification of the pathogen. | We raised monoclonal antibodies (MAbs) against Vibrio parahaemolyticus cell extracts. One of the MAbs, designated MAb-VP34, reacted in enzyme-linked immunosorbent assays (ELISAs) with 140 V. parahaemolyticus strains, regardless of serotype or origin. MAb-VP34 did not detectably react with 96 strains belonging to 27 other Vibrio species (except for Vibrio natriegens) or with 29 non-Vibrio species. These results show that MAb-VP34 is highly specific for V. parahaemolyticus. Western blotting and ma ... | 2012 | 22056232 |
| Vibrio jasicida sp. nov., a member of the Harveyi clade, from marine animals (packhorse lobster, abalone, and Atlantic salmon). | Six isolates of a facultatively anaerobic bacterium were recovered in culture from marine invertebrates and vertebrates, including packhorse lobster (Jasus verreauxi), abalone (Haliotis sp.) and Atlantic salmon (Salmo salar) between 1994 and 2002. The bacteria are Gram-negative, rod shaped and motile by means of more than one polar flagellum, oxidase-positive, catalase-positive and able to grow between 0.5-8.0% NaCl (optimum 3.0-6.0%) and at temperatures of 10-37 °C (optimum 25-30 °C). On the ba ... | 2011 | 21984666 |
| molar growth yields, respiration and cytochrome patterns of beneckea natriegens when grown at different medium dissolved-oxygen tensions. | the effect of medium dissolved-oxygen tension on the molar growth yield, respiration and cytochrome content of beneckea natriegens in chemostat culture (d 0-37 h-1) was examined. the molar growth yield (y), the specific rate of oxygen (qo2) and glucose consumption, and the specific rate of carbon dioxide evolution were independent of the dissolved-oxygen tension above a critical value (greatest than 2 mmhg). however, the potential respiration rate increased with reduction in the dissolved-oxygen ... | 1975 | 1194892 |
| external separation, internal ligation and injection in circumferential mixed hemorrhoids. analysis of 202 cases. | | 2001 | 98305 |
| ec directives on medical devices. | | 2006 | 1392944 |
| exclusion testing for huntington's disease in pregnancy with a closely linked dna marker. | 55 couples where one partner was at 50% risk of huntington's disease (hd) were investigated with a dna probe closely linked to hd, with a view to exclusion testing in a future pregnancy. in 3 of 9 pregnancies so far, hd was excluded in the absence of recombination. in 3 the risk was raised to around 50%, and in 2 exclusion tests were uninformative. the remaining couple changed their minds about termination of the pregnancy and the test was therefore judged inappropriate. | 2010 | 2884411 |
| [duodenal gastrinoma. description of a clinical case]. | | 2011 | 3231322 |
| the piriformis syndrome. | | 2012 | 3390766 |
| the kidney in sickle cell disease ii: the kidney and sickling phenomenon. | | 2016 | 4805648 |
| [conservative treatment of injuries of the liver]. | | 2014 | 3911104 |
| [preparation and purification of anti-ferritin, anti-cytochrome b 5 hybrid antibodies]. | | 2014 | 4122927 |
| construction of a bioreporter by heterogeneously expressing a vibrio natriegens reca::luxcdabe fusion in escherichia coli, and genotoxicity assessments of petrochemical-contaminated groundwater in northern china. | here, we constructed an escherichia coli reca::luxcdabe bioreporter for genotoxicity assessments. the reca promoter was cloned from the marine bacterium vibrio natriegens. this bioreporter showed a dose-response relationship following induction by mitomycin c, and other pollutants or environmental samples could be calculated as mitomycin c equivalents, which provided a way to quantitatively compare the genotoxicities of different environmental samples. this bioreporter was used to evaluate the g ... | 2016 | 27258332 |
| hepatitis b antigen in a dialysis unit: five years experience. | | 2005 | 545706 |
| animal rights/liberation philosophy. | | 2006 | 1563603 |
| the case for the proposed increase in iron enrichment of flour and wheat products. | | 2015 | 5023246 |
| genes similar to the vibrio parahaemolyticus virulence-related genes tdh, tlh, and vscc2 occur in other vibrionaceae species isolated from a pristine estuary. | detection of the human pathogen vibrio parahaemolyticus often relies on molecular biological analysis of species-specific virulence factor genes. these genes have been employed in determinations of v. parahaemolyticus population numbers and the prevalence of pathogenic v. parahaemolyticus strains. strains of the vibrionaceae species photobacterium damselae, vibrio diabolicus, vibrio harveyi, and vibrio natriegens, as well as strains similar to vibrio tubiashii, were isolated from a pristine salt ... | 2014 | 24212573 |
| vibrio natriegens as a fast-growing host for molecular biology. | a rapidly growing bacterial host would be desirable for a range of routine applications in molecular biology and biotechnology. the bacterium vibrio natriegens has the fastest growth rate of any known organism, with a reported doubling time of <10 min. we report the development of genetic tools and methods to engineer v. natriegens and demonstrate the advantages of using these engineered strains in common biotech processes. | 2016 | 27571549 |
| [screening and identification of a bacterium capable of converting agar to neoagaro oligosaccharides]. | to screen and identify a bacterium capable of converting agar to neoagaro oligosaccharides. | 2015 | 26762025 |
| universally improving effect of mixed electron donors on the co₂ fixing efficiency of non-photosynthetic microbial communities from marine environments. | the universality of improved co₂ fixing upon the addition of mixed electron donors (meds) composed of na₂s, no₂(-), and s₂o₃(2-) to non-photosynthetic microbial communities (npmcs) obtained from 12 locations in four oceans of the world was validated. the co₂ fixing efficiencies of npmcs were universally enhanced by med compared with those obtained using h₂ alone as electron donor, with average increase of about 276%. an increase in microbial inoculation concentration could increase the net amoun ... | 2014 | 25108727 |
| draft genome sequence of the fast-growing bacterium vibrio natriegens strain dsmz 759. | vibrio natriegens is a gram-negative bacterium known for its extremely short doubling time. here we present the annotated draft genome sequence of vibrio natriegens strain dsmz 759, with the aim of providing insights about its high growth rate. | 2013 | 23969053 |
| draft genome sequence of the fast-growing marine bacterium vibrio natriegens strain atcc 14048. | vibrio natriegens bacteria are gram-negative aquatic microorganisms that are found primarily in coastal seawater and sediments and are perhaps best known for their high growth rates (generation time of <10 min). in this study, we report the first sequenced genome of this species, that of the type strain vibrio natriegens atcc 14048, a salt marsh mud isolate from sapelo island, ga. | 2013 | 23929482 |
| specific atp and specific oxygen uptake rate in immobilized cell aggregates: experimental results and theoretical analysis using a structured model of immobilized cell growth. | in this work the quality and activity of immobilized beneckea natriegens have been measured using specific atp (satp) [mg (atp) g(-1) (dry biomass)] and specific oxygen uptake rate (sour) [mg o(2) g(-1) (dry biomass) h(-1)]. the cells were grown in a 3 l three phase air lift bioreactor (tpalb) and were immobilized on diatomaceous earth (silica) support particles; sterile conditions were employed during the experiment, with n-propanol as the sole carbon source. two sets of experiments were perfor ... | 1997 | 18636576 |
| adenylate energy charge during batch culture of beneckea natriegens. | the value of the adenylate energy charge, i.e. ([atp] + 1/2[adp])/([atp] + [adp] + [amp]), during batch culture of beneckea natriegens remained relatively constant during the exponential and early stationary phases of the growth cycle. during exponential growth the intracellular atp content remained constant, the amount of atp in the culture increasing proportionally with growth; these conditions were unaltered during growth in the presence of added cyclic amp. on cessation of growth, significan ... | 1977 | 13148 |
| the effect of rate of respiration on sensitivity to cyanide and carbon monoxide in beneckea natriegens grown in batch and continuous culture. | | 1976 | 1278391 |
| multiplex genome editing by natural transformation (mugent) for synthetic biology in vibrio natriegens. | vibrio natriegens has recently emerged as an alternative to escherichia coli for molecular biology and biotechnology, but low-efficiency genetic tools hamper its development. here, we uncover how to induce natural competence in v. natriegens and describe methods for multiplex genome editing by natural transformation (mugent). mugent promotes integration of multiple genome edits at high-efficiency on unprecedented time scales. also, this method allows for generating highly complex mutant populati ... | 2017 | 28571309 |
| environmental impact of tributyltin-resistant marine bacteria in the indigenous microbial population of tributyltin-polluted surface sediments. | we compared the tbt-resistant ability of resting cells prepared from isolates that formed colonies on nutrient agar plates containing 100 µm tributyltin (tbt) chloride, such as photobacterium sp. tky1, halomonas sp. tky2, and photobacterium sp. ngy1, with those from taxonomically similar type strains. photobacterium sp. tky1 showed the highest ability among those three isolates. the number of surviving photobacterium sp. tky1 cells was hardly decreased after 1 h of exposure to 100 µm tbtcl, rega ... | 2017 | 28659560 |
| sulfated phenolic acids from dasycladales siphonous green algae. | sulfated aromatic acids play a central role as mediators of chemical interactions and physiological processes in marine algae and seagrass. among others, dasycladus vermicularis (scopoli) krasser 1898 uses a sulfated hydroxylated coumarin derivative as storage metabolite for a protein cross linker that can be activated upon mechanical disruption of the alga. we introduce a comprehensive monitoring technique for sulfated metabolites based on fragmentation patterns in liquid chromatography/mass sp ... | 2015 | 26188914 |
| sulfation mediates activity of zosteric acid against biofilm formation. | zosteric acid (za), a metabolite from the marine sea grass zostera marina, has attracted much attention due to its attributed antifouling (af) activity. however, recent results on dynamic transformations of aromatic sulfates in marine phototrophic organisms suggest potential enzymatic desulfation of metabolites like za. the activity of za was thus re-investigated using biofilm assays and simultaneous analytical monitoring by liquid chromatography/mass spectrometry (lc/ms). comparison of za and i ... | 2015 | 25915112 |
| the mosaic accessory gene structures of the sxt/r391-like integrative and conjugative elements derived from vibrio spp. isolated from aquatic products and environment in the yangtze river estuary, china. | the emergence, resurgence and spread of human food-borne pathogenic vibrios are one of the major contributors to disease burden and mortality particularly in developing countries with disputable sanitary conditions. previous research on pathogenic vibrio cholerae and vibrio parahaemolitycus derived from clinical samples has proposed links between acquisition of virulence and multiple drug resistance traits and intercellular transmissibility of mobile genetic elements in the environment. to date, ... | 2013 | 24074349 |