Publications

site-directed mutagenesis of a novel serine arylesterase from vibrio mimicus identifies residues essential for catalysis.	site-directed mutagenesis (sdm) of an arylesterase (the arylesterase) from vibrio mimicus revealed that residues s29, h153, and d96 constituted a catalytic triad. the use of a serine residue for ester hydrolysis by the arylesterase proves that the enzyme is a novel serine arylesterase. sdm also showed that d28 was necessary for the esterase activity; to our knowledge it is the first time that a residue immediately preceding the active-site serine in esterases was shown biochemically to possess s ...	1996	8619880
expression of virulence-related properties by, and intestinal adhesiveness of, vibrio mimicus strains isolated from aquatic environments.	a study of the major pathogenic characteristics of vibrio mimicus was carried out with 77 strains isolated from aquatic environments in okayama, japan. of the strains tested, 96% demonstrated in vitro adherence to the rabbit intestinal mucosa, of which 36, 20, and 43% belonged to the strongly, moderately, and weakly adhesive groups, respectively. of the 27 strains which appeared to be enterotoxigenic in the experiments using rabbit ileal loops, 74% belonged to the strongly adhesive group. all st ...	1996	8837444
vibrio mimicus diarrhea following ingestion of raw turtle eggs.	clinical and epidemiological characteristics of diarrhea associated with vibrio mimicus were identified in 33 hospitalized patients referred to the costa rican national diagnostic laboratory network between 1991 and 1994. the relevant symptoms presented by patients included abundant watery diarrhea, vomiting, and severe dehydration that required intravenous dhaka solution in 83% of patients but not fever. seroconversion against v. mimicus was demonstrated in four patients, from whom acute- and c ...	1996	8919774
purification and characterization of novel hemagglutinins from vibrio mimicus: a 39-kilodalton major outer membrane protein and lipopolysaccharide.	two hemagglutinins (has) mediating the agglutinability to rabbit erythrocytes were isolated from 32-h culture supernatant of enterotoxigenic strain e-33 of vibrio mimicus by ultrafiltration followed by gel filtration and anion-exchange column chromatography. the has were designated r-ha and c-ha on the basis of specific hemagglutinating activity towards rabbit erythrocytes only (r-ha) and towards chicken and rabbit erythrocytes (c-ha). sodium dodecyl sulfate-polyacrylamide gel electrophoresis an ...	1996	8926065
toxigenicity & drug sensitivity of vibrio mimicus isolated from patients with diarrhoea.	in a total of 720 faecal specimens from patients with secretory diarrhoea, vomiting, dehydration, gastroenteritis, cholera and cholera like illnesses, 18 strains of v. mimicus were isolated as pure culture. these were characterized for various toxin types and virulence factors using conventional in vitro and in vivo assays. labile and stable toxins were elaborated by 15 and 2 strains respectively by ligated rabbit ileal loop (ril) and suckling mouse assays. while 15 of the whole cell culture ela ...	1996	8996933
analysis of the structural gene encoding a hemolysin in vibrio mimicus.	an environmental isolate of v. mimicus, strain e-33, has been reported to produce and secrete a hemolysin of 63 kda. the hemolysin is enterotoxic in test animals. the nucleotide sequence of the structural gene of the hemolysin was determined. we found a 2,232 bp open reading frame, which codes a peptide of 744 amino acids, with a calculated molecular weight of 83,903 da. the sequence for the structural gene was closely related to the v. cholerae el tor hlya gene, coding an exocellular hemolysin. ...	1997	9087960
purification and characterization of a hemolysin produced by vibrio mimicus.	vibrio mimicus is a causative agent of human gastroenteritis. this pathogen secretes a pore-forming toxin, v. mimicus hemolysin (vmh), which causes hemolysis by three sequential steps: binding to an erythrocyte membrane, formation of a transmembrane pore, and disruption of the cell membrane. vmh with a molecular mass of 63 kda was purified by ammonium sulfate precipitation and column chromatography with phenyl sepharose hp and superose 6 hr. the hemolytic reaction induced by vmh continued up to ...	1997	9125568
nucleotide sequence of the vmha gene encoding hemolysin from vibrio mimicus.	the structural gene (vmha) of hemolysin from vibrio mimicus (atcc33653) was cloned and sequenced. the vmha gene contains an open reading frame consisting of 2232 nucleotides which can code for a protein of 744 amino acids with a predicted molecular mass of 83,059. the similarity of amino acid sequence shows 81.6% identity with vibrio cholerae el tor hemolysin.	1997	9128176
vibrio mimicus attaches to the intestinal mucosa by outer membrane hemagglutinins specific to polypeptide moieties of glycoproteins.	vibrio mimicus is the closest organism to vibrio cholerae. v. mimicus e-33, which is a highly adhesive and enteropathogenic strain, is known to produce three types of hemagglutinins (has), i.e., a 31-kda exocellular metalloprotease (vm-ha/protease), lipopolysaccharide (vm-lpsha), and a 39-kda major outer membrane protein (vm-ompha). hemagglutination induced by vm-lpsha and vm-ompha was inhibited by glycoproteins, including mucin, fetuin, and asialofetuin, but not by monosaccharides, disaccharide ...	1997	9284134
[distribution of serogroups of vibrio cholerae non-o1 non-o139 with specific reference to their ability to produce cholera toxin, and addition of novel serogroups].	a total of 1898 strains of vibrio cholerae non-o1 non-o139, which had been collected worldwide for the past 3 year period of 1994-1996, were serogrouped. the strains were also examined for presence of cholera toxin (ct) gene (ctx) and nag-st gene, and strains which carried to ctx were further analyzed for their ability to produce ct. in addition, attempts were made to establish novel serogroups for those serologically untypable strains. of those examined, 1,774 strains of v. cholerae non-o1 non- ...	1997	9394556
structural analysis of the o-antigenic polysaccharide from vibrio mimicus n-1990.	the o-antigenic polysaccharide part of the lipopolysaccharide from vibrio mimicus n-1990 has been investigated. sugar and methylation analysis of native and dephosphorylated polysaccharide together with nmr spectroscopy show that the polysaccharide is composed of tetrasaccharide repeating units. the structure of the repeating unit of the polysaccharide from v. mimicus n-1990 could be determined as: -->4)-alpha-d-galpnac-(1-->3)-beta-d-galpnac-(1-->2)-4,6-p-beta-d-galp-( 1-->3)-alpha-d-galpnac-(1 ...	1998	9490076
isolation and sequence analysis of metalloprotease gene from vibrio mimicus.	the vmc gene encoding a metalloprotease of vibrio mimicus (atcc 33653) was cloned in escherichia coli and sequenced. the vmc gene contained 1884 nt sequence which codes a polypeptide of 628 amino acids with a predicted molecular mass of 71,275 da. the deduced amino acid sequence had the similarity of 68.5% with v. parahaemolyticus metalloprotease. the consensus sequence of a zinc binding motif (hexxh) was identified to be heyth. the zymography analysis showed a gelatinolytic protein band around ...	1998	9602021
cloning and identification of a phospholipase gene from vibrio mimicus.	the phospholipase gene phl was identified from vibrio mimicus (atcc33653) and sequenced. the entire open reading frame (orf) was composed of 1410 nucleotides and encoding 470 amino acids. the phl was placed upstream of hemolysin gene (vmha) with opposite direction of transcription. from the blast search program, the deduced amino acids sequence showed 74.4% identity with phospholipase gene (lec) from v. cholerae el tor. the entire orf of phospholipase gene was amplified by pcr and inserted into ...	1998	9767128
optimization of excimer-forming two-probe nucleic acid hybridization method with pyrene as a fluorophore.	a previously presented homogeneous assay method, named the excimer-forming two-probe nucleic acid hybridization (etph) method, is based on specific excimer formation between two pyrenes attached at the neighboring terminals of two sequential probe oligonucleotides complementary to a single target. in this study, we investigated assay conditions and optimal molecular design of probes for intense excimer emission using a pyrenemethyliodoacetamide-introduced 16mer probe, a pyrene butanoic acid-intr ...	1998	9826766
sources of vibrio mimicus contamination of turtle eggs.	vibrio mimicus contamination of sand increased significantly during the arrival of the olive ridley sea turtles (lepidochelys olivacea) at ostional anidation beach, costa rica. statistical analysis supports that eggs are contaminated with v. mimicus by contact with the sand nest. v. mimicus was isolated from eggs of all nests tested, and ctxa+ strains were found in 31% of the nests, all of which were near the estuary.	1999	9872804
detection of genes encoding cholera toxin (ct), zonula occludens toxin (zot), accessory cholera enterotoxin (ace) and heat-stable enterotoxin (st) in vibrio mimicus clinical strains.	a total of 51 clinical strains of vibrio mimicus were searched for the presence of virulence-associated genes, like ctx, zot or ace genes which locate in "cholera virulence cassette," and the st gene by polymerase chain reaction. moreover, the pathological potential of each clinical strain was also examined by rabbit ileal loop (ril). three strains showed to have the ctx gene, of which only one strain was zot gene-positive. meanwhile, one other strain was zot+ but ctx-. all of these four strains ...	1998	10037216
analysis of 16s-23s rrna intergenic spacer regions of vibrio cholerae and vibrio mimicus.	vibrio cholerae identification based on molecular sequence data has been hampered by a lack of sequence variation from the closely related vibrio mimicus. the two species share many genes coding for proteins, such as ctxab, and show almost identical 16s dna coding for rrna (rdna) sequences. primers targeting conserved sequences flanking the 3' end of the 16s and the 5' end of the 23s rdnas were used to amplify the 16s-23s rrna intergenic spacer regions of v. cholerae and v. mimicus. two major (c ...	1999	10224020
response of pathogenic vibrio species to high hydrostatic pressure.	vibrio parahaemolyticus atcc 17802, vibrio vulnificus atcc 27562, vibrio cholerae o:1 atcc 14035, vibrio cholerae non-o:1 atcc 14547, vibrio hollisae atcc 33564, and vibrio mimicus atcc 33653 were treated with 200 to 300 mpa for 5 to 15 min at 25 degrees c. high hydrostatic pressure inactivated all strains of pathogenic vibrio without triggering a viable but nonculturable (vbnc) state; however, cells already existing in a vbnc state appeared to possess greater pressure resistance.	1999	10347079
cross-reaction between a strain of vibrio mimicus and v. cholerae o139 bengal.	of 200 isolates of vibrio mimicus screened, one from water (n-57) agglutinated with v. cholerae o139 polyclonal antiserum (absorbed with a rough strain of v. cholerae only) and not with o139 polyclonal diagnostic antiserum (absorbed with the rough strain and v. cholerae o22 and o155). the antigenic relationship between v. cholerae 0139 and n-57 is of a,b-a,c type, where a is the common antigenic epitope and b and c are unique epitopes. strain n-57 was assigned to a new serogroup of v. cholerae o ...	1999	10482300
lysogenic conversion of environmental vibrio mimicus strains by ctxphi.	the filamentous bacteriophage ctxphi, which encodes cholera toxin (ct) in toxigenic vibrio cholerae, is known to propagate by infecting susceptible strains of v. cholerae by using the toxin coregulated pilus (tcp) as its receptor and thereby causing the origination of new strains of toxigenic v. cholerae from nontoxigenic progenitors. besides v. cholerae, vibrio mimicus strains which are normally tcp negative have also been shown to occasionally produce ct and cause diarrhea in humans. we analyz ...	1999	10531221
infectious ctxphi and the vibrio pathogenicity island prophage in vibrio mimicus: evidence for recent horizontal transfer between v. mimicus and v. cholerae.	vibrio mimicus differs from vibrio cholerae in a number of genotypic and phenotypic traits but like v. cholerae can give rise to diarrheal disease. we examined clinical isolates of v. mimicus for the presence of ctxphi, the lysogenic filamentous bacteriophage that carries the cholera toxin genes in epidemic v. cholerae strains. four v. mimicus isolates were found to contain complete copies of ctxphi. southern blot analyses revealed that v. mimicus strain pt5 contains two ctx prophages integrated ...	2000	10678967
characterization of an arylesterase from lactobacillus helveticus cnrz32.	an esterase gene (esta) was isolated from a previously constructed genomic library of lactobacillus helveticus cnrz32. the esta gene consisted of a 558 bp open reading frame encoding a putative peptide of 21.3 kda. protein sequence homology searches using blast revealed that esta had low amino acid sequence identity with the serine-dependent arylesterases tesi (24%) and etpa (26%) from escherichia coli and vibrio mimicus, respectively. a recombinant esta fusion protein containing a c-terminal si ...	2000	10792515
analysis of vibrio mimicus clinical strains by arbitrarily primed polymerase chain reaction.	a total of 51 vibrio mimicus clinical strains from different geographic locations were examined by arbitrarily primed polymerase chain reaction (ap-pcr). the primer vmh-3 divided them into 28 groups, although 18 groups consisted of a single strain at present. all groups had a common 1.0-kb amplification fragment. most of the groups consisted of strains from same region, although two exceptional groups showed a few amplification fragments including strains from different regions. ap-pcr groups we ...	2000	10803502
the vibrio cholerae o1 chromosomal integron.	until the discovery of the vibrio cholerae repeat (vcr), the gene capture and expression systems termed integrons had been typically associated with antibiotic-resistance gene cassettes with usually less than five genes in an array. a method is described for the cloning of the ends of large cassette arrays. conserved restriction sites within vcrs facilitated the mapping by southern hybridization and cloning of the 5' end of the vcr array, and using appropriate fragments it was possible to develo ...	2000	11021935
identification and characterization of the soda genes encoding manganese superoxide dismutases in vibrio parahaemolyticus, vibrio mimicus, and vibrio vulnificus.	sequencing of fur titration assay-positive clones obtained from genomic dna libraries of vibrio parahaemolyticus, v. mimicus and v. vulnificus revealed open reading frames encoding proteins of 202, 205 and 202 amino acid residues, respectively. each open reading frame was preceded by a predicted fur box which overlaps a likely promoter with similarity to the -10 and -35 consensus sequence of escherichia coli. the deduced amino acid sequences shared considerable homology with bacterial mn-contain ...	2001	11293479
differentiation of environmental and clinical isolates of vibrio mimicus from vibrio cholerae by multilocus enzyme electrophoresis.	in this study, we demonstrated that analyzed strains of vibrio mimicus and vibrio cholerae could be separated in two groups by using multilocus enzyme electrophoresis (mee) data from 14 loci. we also showed that the combination of four enzymatic loci enables us to differentiate these two species. our results showed that the ribosomal intergenic spacer regions pcr-mediated identification system failed, in some cases, to differentiate between v. mimicus and v. cholerae. on the other hand, mee prov ...	2001	11319123
synthesis of esculentin-1 antibacterial peptide fragments on 1,4-butanediol dimethacrylate cross-linked polystyrene support.	peptide segments corresponding to antibacterial esculentin-1 (1-15), (3344), (9-27), and their modified forms were synthesized on 1,4-butanediol dimethacrylate cross-linked polystyrene (ps-bdodma) support. hydroxymethyl and aminomethyl 2% ps-bdodma supports were used for the synthesis. the hmpb linker was appended to the aminomethyl resin using hbtu in presence of hobt and the first amino acid was incorporated using msnt. the conventional fmoc synthetic protocol was used for the synthesis of pep ...	2001	11330345
characterization of aeromonas and vibrio species isolated from a drinking water reservoir.	to study the phenotypic and chemotaxonomic (i.e. phospholipid and cellular fatty acid composition) characteristics of environmental aeromonas spp. and vibrio spp. isolated from a drinking water reservoir near vladivostok city, and the application of some chemotaxonomic markers for discrimination of the two genera and species.	2001	11412322
[vibrio mimicus gastroenteritis: report of 2 cases].		2001	11488102
detection of virulence associated genes in clinical strains of vibrio mimicus.	a total of 42 clinical strains of vibrio mimicus were examined for the presence of virulence associated genes toxr, toxs, toxt, tcpp, ctx and tcpa by pcr assay. almost all strains were shown to have the toxr gene, while the toxs gene was found in 27 strains. on the other hand, five strains possessed both toxt and tcpp genes, but others had neither. only two strains were positive for amplification of the ctx gene, whereas no pcr product with tcpa primers was detected. the results indicate the inc ...	2001	11592635
analysis of 16s-23s rrna intergenic spacer of vibrio cholerae and vibrio mimicus for detection of these species.		2002	11692861
characterization of vibrio mimicus phospholipase a (phla) and cytotoxicity on fish cell.	vibrio mimicus is a typical strain of vibrio cholerae and produces a phospholipase (phla) which shares a highly conserved amino acid sequence with the lecithinase (lec) of v. cholerae. the recombinant protein (rphla) produced from the phla gene of v. mimicus was expressed in escherichia coli as his-tag fused protein. the rphla was purified by gel filtration and ni-metal affinity chromatographies. when the action mode was investigated by tlc and gc-ms, the purified rphla protein showed a phosphol ...	2002	12387827
seasonality of chesapeake bay bacterioplankton species.	bacteria, gamma-subclass of proteobacteria, vibrio-photobacterium, vibrio vulnificus, vibrio cholerae-vibrio mimicus, and vibrio cincinnatiensis in water samples collected from the choptank river in chesapeake bay from 15 april to 16 december 1996 were enumerated using a fluorescent oligonucleotide direct-counting (fodc) procedure. fodc results obtained using a bacteria taxon-specific probe ranged from one-third the number of to the same number as that obtained by the acridine orange direct coun ...	2002	12406742
bacteria of the gamma-subclass proteobacteria associated with zooplankton in chesapeake bay.	the seasonal abundance of gamma-subclass proteobacteria, vibrio-photobacterium, vibrio cholerae-vibrio mimicus, vibrio cincinnatiensis, and vibrio vulnificus in the choptank river of chesapeake bay associated with zooplankton was monitored from april to december 1996. large (>202- microm) and small (64- to 202- microm) size classes of zooplankton were collected, and the bacteria associated with each of the zooplankton size classes were enumerated by fluorescent oligonucleotide direct count. larg ...	2002	12406743
development of a hexaplex pcr assay for rapid detection of virulence and regulatory genes in vibrio cholerae and vibrio mimicus.	we have developed a hexaplex pcr assay for rapid detection of the virulence and regulatory genes for cholera toxin enzymatic subunit a (ctxa), zonula occludens toxin (zot), accessory cholera enterotoxin (ace), toxin-coregulated pilus (tcpa), outer membrane protein (ompu), and central regulatory protein toxr (toxr) in vibrio cholerae and vibrio mimicus. this hexaplex pcr proved successful in screening pathogenic-toxigenic and nonpathogenic-nontoxigenic v. cholerae and v. mimicus strains from both ...	2002	12409420
purification and characterization of enterotoxigenic el tor-like hemolysin produced by vibrio fluvialis.	the halophilic bacterium vibrio fluvialis is an enteric pathogen that produces an extracellular hemolysin. this hemolysin was purified to homogeneity by using sequential hydrophobic-interaction chromatography with phenyl-sepharose cl-4b and gel filtration with sephacryl s-200. it has a molecular weight of 63,000 and an isoelectric point of 4.6, and its hemolytic activity is sensitive to heat, proteases, and preincubation with zinc ions. the hemolysin lyses erythrocytes of the eight different ani ...	2003	12761101
characterization of the enzyme activity of an extracellular metalloprotease (vmc) from vibrio mimicus and its c-terminal deletions.	to investigate the enzymatic properties of vibrio mimicus metalloprotease, the mature metalloprotease gene (vmc) was overexpressed in escherichia coli and the recombinant protein (rvmc61) was purified by metal affinity chromatography. rvmc61 showed maximum activity at about 37 degrees c, ph 8. the purified rvmc61 was very specific toward collagen substrates, such as gelatin, type i, ii, and iii collagens and synthetic peptides (cbz-gplgp and cbz-gpggpa). but it did not show degrading activity to ...	2003	12829301
accuracy of six commercially available systems for identification of members of the family vibrionaceae.	six commercially available bacterial identification products were tested with vibrio alginolyticus (12 strains), v. cholerae (30 strains), photobacterium (vibrio) damselae (10 strains), v. fluvialis (10 strains), v. furnissii (4 strains), v. hollisae (10 strains), v. metschnikovii (9 strains), v. mimicus (10 strains), v. parahaemolyticus (30 strains), and v. vulnificus (10 strains) to determine the accuracy of each system for identification. the products included api 20e, crystal e/nf, microscan ...	2003	14662957
use of reca as an alternative phylogenetic marker in the family vibrionaceae.	this study analysed the usefulness of reca gene sequences as an alternative phylogenetic and/or identification marker for vibrios. the reca sequences suggest that the genus vibrio is polyphyletic. the high heterogeneity observed within vibrios was congruent with former polyphasic taxonomic studies on this group. photobacterium species clustered together and apparently nested within vibrios, while grimontia hollisae was apart from other vibrios. within the vibrios, vibrio cholerae and vibrio mimi ...	2004	15143042
identification and characterization of two contiguous operons required for aerobactin transport and biosynthesis in vibrio mimicus.	in response to iron deprivation, vibrio mimicus produces aerobactin as a major siderophore. application of the fur titration assay to a v. mimicus genomic dna library followed by further cloning of the surrounding regions led to the identification of two adjacent, iron-regulated operons. one contains three genes encoding homologs of the escherichia coli fhucdb and the other, five genes encoding homologs of the e. coli iucabcd iuta. construction of the v. mimicus polar disruptants in the respecti ...	2004	15215626
distribution of virulence-associated genes in vibrio mimicus isolates from clinical and environmental origins.	distribution of virulence-associated genes in vibrio mimicus was studied including the toxin genes ctxa, tdh, st and vmh and the genes necessary for regulation of toxin production, toxr, toxs, toxt, tcpa and tcpp. approximately half of clinical v. mimicus isolates possessed one or more genes encoding v. cholerae enterotoxic factors such as ctxa, tdh and st. all of the clinical and environmental isolates possessed vmh encoding v. mimicus hemolysin (vmh). the ctxa encoding cholera toxin was detect ...	2004	15272201
the ability of two different vibrio spp. bacteriophages to infect vibrio harveyi, vibrio cholerae and vibrio mimicus.	to determine the host range of the vibrio harveyi myovirus-like bacteriophage (vhml) and the cholera toxin conversion bacteriophage (ctx phi) within a range of vibrio cholerae and v. mimicus and v. harveyi, v. cholerae and v. mimicus isolates respectively.	2004	15357715
evolutionary genetic analysis of the emergence of epidemic vibrio cholerae isolates on the basis of comparative nucleotide sequence analysis and multilocus virulence gene profiles.	vibrio cholerae, the causative agent of cholera, is a natural inhabitant of the aquatic ecosystem. we examined a unique collection of v. cholerae clinical and environmental isolates of widespread geographic distribution recovered over a 60-year period to determine their evolutionary genetic relationships based on analysis of two housekeeping genes, malate dehydrogenase (mdh) and a chaperonin (groel). in addition, the phylogenetic distribution of 12 regions associated with virulence was determine ...	2004	15472325
molecular evolution of vibrio pathogenicity island-2 (vpi-2): mosaic structure among vibrio cholerae and vibrio mimicus natural isolates.	vibrio cholerae is a gram-negative rod that inhabits the aquatic environment and is the aetiological agent of cholera, a disease that is endemic in much of southern asia. the 57.3 kb vibrio pathogenicity island-2 (vpi-2) is confined predominantly to toxigenic v. cholerae o1 and o139 serogroup isolates and encodes 52 orfs (vc1758 to vc1809), which include homologues of an integrase (vc1758), a restriction modification system, a sialic acid metabolism gene cluster (vc1773-vc1783), a neuraminidase ...	2005	15632448
a hemolysin of vibrio mimicus (vmh) stimulates cells to produce atp and cyclic amp which appear to be secretory mediators.	the hemolysin of vibrio mimicus(vmh) is a pore-forming toxin with both enterotoxic and hemolytic activity. the hemolysis by vmh is induced by creation of pores in the membrane of erythrocyte; however, the mechanism for the enterotoxic action of vmh has remained unclear. in order to clarify the mechanism, we incubated t84 cells (a human colon carcinoma cell line) with vmh and found that the levels of atp and cyclic amp of culture medium increased after exposure of the cells to vmh. subsequently, ...	2005	15665456
molecular typing of epidemic and nonepidemic vibrio cholerae isolates and differentiation of v. cholerae and v. mimicus isolates by pcr-single-strand conformation polymorphism analysis.	to examine the utility of polymerase chain reaction (pcr)-single-strand conformation polymorphism (sscp) analysis to differentiate epidemic and nonepidemic vibrio cholerae isolates as well as to differentiate v. cholerae and vibrio mimicus isolates.	2005	15715856
characterization of fe/mn-superoxide dismutase from diatom thallassiosira weissflogii: cloning, expression, and property.	a cdna clone of 1114 bp encoding a putative mn-superoxide dismutase (mn-sod) from diatom thallassiosira weissflogii was cloned by the pcr technique. nucleotide sequence analysis of this cdna clone revealed that it was translated into 201 amino acid residues. when the sequence was compared with mn-sods from vibrio mimicus and escherichia coli, as well as two fe-sods from e. coli and photobacterium leiognathi, this sod showed higher homology to mn-sod. the amino acid residues required to coordinat ...	2005	15740026
the faxwxxt motif in the carboxyl terminus of vibrio mimicus metalloprotease is involved in binding to collagen.	we have shown previously that the c-terminal region of the extracellular metalloprotease of vibrio mimicus (vmc) is essential for collagenase activity. here, we demonstrate that deletion of 100 amino acids, but not 67 amino acids, from the c-terminus of the intact vmc protein (vmc61) abolished the collagenase activity. the intervening 33-amino acid region contains a repeated faxwxxt motif that is essential for insoluble type i collagen binding; the isolated 33-amino acid peptide bound to insolub ...	2005	15848196
[pathogenic factors of vibrios with special emphasis on vibrio vulnificus].	bacteria of the genus vibrio are normal habitants of the aquatic environment and play roles for biocontrole of aquatic ecosystem, but some species are believed to be human pathogens. these species can be classified into two groups according to the types of diseases they cause: the gastrointestinal infections and the extraintestinal infections. the pathogenic species produce various pathogenic factors including enterotoxin, hemolysin, cytotoxin, protease, siderophore, adhesive factor, and hemaggl ...	2005	15997210
selectivity and specificity of a chromogenic medium for detecting vibrio parahaemolyticust.	the thiosulfate-citrate-bile salts-sucrose agar (tcbs) used in the most-probable-number method for detecting vibrio parahaemolyticus cannot differentiate growth of v. parahaemolyticus from vibrio vulnificus or vibrio mimicus. this study examined the selectivity and specificity of bio-chrome vibrio medium (bcvm), a chromogenic medium that detects v. parahaemolyticus on the basis of the formation of distinct purple colonies on the medium. a panel consisting of 221 strains of bacteria, including 17 ...	2005	16013386
biochemical characterization of a cambialistic superoxide dismutase isozyme from diatom thallassiosira weissflogii: cloning, expression, and enzyme stability.	a cdna clone of 1081 bp encoding a second putative superoxide dismutase (sod) from diatom thallassiosira weissflogii was cloned by the polymerase chain reaction technique. the cdna encodes a protein of 286 amino acid residues. alignment of the truncated sod sequence containing 217 amino acid residues with mn-sods from vibrio mimicus and escherichia coli, as well as two fe-sods from e. coli and photobacterium leiognathi, this sod showed greater homology to mn-sod. the residues required to coordin ...	2005	16076113
organization of the ctx prophage in environmental isolates of vibrio mimicus.	the organization of the ctx prophage in environmental strains of vibrio mimicus was investigated. sixteen hundred non-sucrose fermenting vibrios were examined for ctx gene by hybridization. out of 1,600 isolates, 6 v. mimicus isolates contained ctxa gene. the organization of ctx prophage was determined by rflp using ctxa probe. the ctx prophage integrated at a single site in v. mimicus genome which was present as a single copy flanked by at least a single rs element. ribotype pattern revealed th ...	2005	16113506
use of oligonucleotide array for identification of six foodborne pathogens and pseudomonas aeruginosa grown on selective media.	identification of presumptive foodborne pathogens grown on selective media may take one to several days and requires a different battery of biochemical tests for each microorganism. a molecular identification method was developed in which universal primers were used to amplify the 16s to 23s rdna intergenic spacer of target microorganisms, and pcr products were hybridized to a panel of species-specific oligonucleotides that were immobilized on a nylon membrane. the seven target microorganisms we ...	2005	16300063
ecology of vibrio mimicus in aquatic environments.	[this corrects the article on p. 2077 in vol. 55.].	1990	16348134
high prevalence of vibrio cholerae non-o1 carrying heat-stable-enterotoxin-encoding genes among vibrio isolates from a temperate-climate river basin of central italy.	vibrio spp. of clinical interest from the arno river basin (tuscany, italy) were investigated in this study. vibrios were isolated from 70% of water samples. vibrio cholerae non-o1 was the most prevalent species (82% of isolates), followed by vibrio mimicus (10%) and vibrio metschnikovii (8%). recovery of vibrios was correlated with temperature, ph, and various indicators of municipal pollution. none of the 150 vibrio isolates carried ctx-related genomic sequences, whereas 18 (14.6%) of the 123 ...	1997	16535661
pcr-based identification of vibrio cholerae and the closely related species vibrio mimicus using the large chromosomal ori sequence of vibrio cholerae.	the bacterial chromosomal replication origin (ori) sequences are a highly conserved essential genetic element. in this study, the large chromosomal replication origin sequence of vibrio cholerae (oricivc) has been targeted for identification of the organism, including the biotypes of serogroup o1. the oricivc sequence-based pcr assay specifically amplified an 890 bp fragment from all the v. cholerae strains examined. a point mutation in the oricivc sequence of the classical biotype of o1 serogro ...	2006	16553836
presence of luxs/ai-2 based quorum-sensing system in vibrio mimicus : luxo controls protease activity.	presence of the quorum-sensing regulation system in vibrio mimicus was investigated. the culture supernatants of v. mimicus strains were found to possess ai-2 autoinducer like activity, and the strains were found to harbor the genes which are homologous to luxs, luxo, and luxr of v. harveyi. these genes of v. harveyi have been shown to be important components of v. harveyi-like quorum-sensing system. the luxo gene homologue known to encode luxo, the central component of the regulation system, wa ...	2006	16714849
development of monoclonal antibodies for simple identification of vibrio alginolyticus.	the present study was aimed to produce monoclonal antibodies (mabs) for simple and specific identification of vibrio alginolyticus infection in shrimp.	2006	16965376
isolation of vibrio mimicus from a case of acute diarrhoea--a case report.	over a four months period (june to september, 2003) 111 stool samples from cases of acute diarrhoea were screened for vibronaceae in department of microbiology, smt. n. h. l. municipal medical college, ahmedabad. all the isolates were identified by standard laboratory technique. a total of 36 species belonging to vibrionaceae were isolated. phage typing of all these vibronaceae were done at national institute of cholera and enteric disease, kolkata. out of 36 isolates, 35 were vibrio cholereae a ...	2006	17001922
haemolysin produced by vibrio mimicus activates two cl- secretory pathways in cultured intestinal-like caco-2 cells.	haemolysin (vmh) is a virulent factor produced by vibrio mimicus, a human pathogen that causes diarrhoea. as intestinal epithelial cells are the primary targets of haemolysin, we investigated its effects on ion transport in human colonic epithelial caco-2 cells. vmh increased the cellular short circuit current (isc), used to estimated ion fluxes, and 125i efflux of the cells. the vmh-induced increases in isc and 125i efflux were suppressed by depleting ca2+ from the medium or by pretreating the ...	2007	17026482
microwave irradiation for shortening the processing time of samples of flagellated bacteria for scanning electron microscopy.	microwave irradiation (mwi) has been applied to the development of rapid methods to process biological samples for scanning electron microscopy (sem). in this paper we propose two simple and quick techniques for processing bacteria (proteus mirabilis and vibrio mimicus) for sem using mwi. in the simplest methodology, the bacteria were placed on a cover-glass, air-dried, and submitted to conductivity stain. the reagent used for the conductivity stain was the mordant of a light microscopy staining ...	2004	17061527
identification of vibrio isolates by a multiplex pcr assay and rpob sequence determination.	vibrio, a diverse genus of aquatic bacteria, currently includes 72 species, 12 of which occur in human clinical samples. of these 12, three species--vibrio cholerae, vibrio parahaemolyticus, and vibrio vulnificus-account for the majority of vibrio infections in humans. rapid and accurate identification of vibrio species has been problematic because phenotypic characteristics are variable within species and biochemical identification requires 2 or more days to complete. to facilitate the identifi ...	2007	17093013
proteolytic activation of vibrio mimicus (vm) major outer membrane protein haemagglutinin (ha) with vm-ha/protease: implication for understanding bacterial adherence.	vibrio mimicus (vm) haemagglutinins (has), such as an extracellular ha/protease (vm-ha/protease) and a major outer membrane protein-ha (vm-ompha), have been recognized as the putative adherence factors for the bacterium. however, the mechanism by which has coordinate the adherence function of the bacterium remains as yet unknown. we report herein the positive interaction between vm-ha/protease and vm-ompha resulting in significant enhancement of the haemagglutinating ability. in this interaction ...	2006	17116978
catecholamine-induced stimulation of growth in vibrio species.	to evaluate the effect of norepinephrine (ne) and related compounds on the growth of bacteria, we have examined the effect of the neuroendocrine hormone ne and related compounds on the growth of vibrio parahaemolyticus and other human-pathogenic vibrio species (vibrio cholerae, vibrio vulnificus, and vibrio mimicus).	2007	17576228
rapid and specific identification of 5 human pathogenic vibrio species by multiplex polymerase chain reaction targeted to dnaj gene.	a multiplex polymerase chain reaction (pcr) method, specifically designed for application in routine diagnostic laboratories, was developed for identifying 5 human pathogen vibrio species: vibrio cholerae, vibrio parahaemolyticus, vibrio vulnificus, vibrio mimicus, and vibrio alginolyticus. this assay directed toward the dnaj gene was tested on a total of 355 strains representing 13 vibrio species and 17 non-vibrio species. specific pcr fragments were produced in isolates belonging to the 5 targ ...	2007	17614235
identification of vibrio cholerae and vibrio mimicus by multilocus sequence analysis (mlsa).	vibrio cholerae and vibrio mimicus have similar phenotypes and genomes making rapid differentiation of these two species difficult. the first standard multilocus sequence analysis (mlsa) scheme for the identification of these species is described. a collection of 45 representative isolates from different geographical regions and hosts was examined using segments of the housekeeping genes pyrh, reca and rpoa. overall, the closest phylogenetic neighbours of these species were vibrio furnissii and ...	2008	18319466
improvement of innate immune responses and defense activity in mitten crab (eriocheir sinensis) by oral administration of beta-glucan.	the beta-glucan (bg), extracted from saccharomyces cerevisiae cell wall, was orally administrated to the mitten crab (eriocheir sinensis) at 0, 1, 5 and 10 g bg/kg diet for 28 days, followed by a challenge with vibrio mimicus by intramuscular injection. growth, phenoloxidase, superoxide dismutase, acid phosphatase and alkaline phosphatase activity were monitored after 14 and 28 days. the results showed an immunomodulatory effect and protection against v. mimicus by dietary supplementation of bg. ...	2008	18568402
a methoxylated fatty acid isolated from the brown seaweed ishige okamurae inhibits bacterial phospholipase a2.	a methoxylated fatty acid that inhibits phospholipase a(2) (pla(2); ec 3.1.1.4) was purified from the brown seaweed ishige okamurae. approximately 8.1 mg of the inhibitory compound, 7-methoxy-9-methylhexadeca-4,8-dienoic acid, was isolated from 1 kg of i. okamurae powder. recombinant pla(2) derived from the pathogenic bacterium vibrio mimicus was used as the target enzyme. the methoxylated fatty acid compound competitively inhibited pla(2) with a ki value of 3.9 microg/ml. the concentrations req ...	2008	18570268
vibrio porteresiae sp. nov., a diazotrophic bacterium isolated from a mangrove-associated wild rice (porteresia coarctata tateoka).	two facultatively anaerobic, nitrogen-fixing bacteria (strains mssrf30(t) and mssrf31) were isolated from a mangrove-associated wild rice (porteresia coarctata tateoka). these strains were determined to be nitrogen-fixers using the acetylene reduction assay and by pcr detection of a nifh gene amplicon. phylogenetic analyses based on 16s rrna gene sequences indicated that the novel strains were most closely related to vibrio fluvialis lmg 7894(t) (96.8 % gene sequence similarity), vibrio furnissi ...	2008	18599703
an incidence of large foodborne outbreak associated with vibrio mimicus.		2009	18925423
expression of a fusion protein containing human epidermal growth factor and the collagen-binding domain of vibrio mimicus metalloprotease.	human epidermal growth factor (hegf) is a polypeptide of 53 amino acids, is an important autocrine/paracrine factor in the human body, and is used in the pharmaceutical and cosmetics industries. we constructed a fusion hegf protein with a collagen-binding domain (cbd) composed of 33 amino acids from vibrio mimicus metalloprotease (vmcbd). the cbd segment of the metalloprotease was fused at the c terminus of the hegf protein. the recombinant fusion protein was expressed in escherichia coli and pu ...	2009	18931974
modulation of vibrio mimicus hemolysin through limited proteolysis by an endogenous metalloprotease.	vibrio mimicus is a causative agent of human gastroenteritis and food poisoning, and this species produces an enterotoxic hemolysin (v. mimicus hemolysin) as a virulence determinant. vibrio mimicus hemolysin is secreted as an 80 kda precursor, which is later converted to the 66 kda mature toxin through removal of an n-terminal propeptide via cleavage of the arg151-ser152 bond. in this article, we investigate the role of the endogenous metalloprotease (v. mimicus protease) in the maturation of v. ...	2009	19143841
mycotic abdominal aortic aneurysm infected by vibrio mimicus: report of a case.	this report describes a case of a mycotic aneurysm of the infrarenal abdominal aorta infected by vibrio mimicus, which is the only such case ever reported in the literature. the 80-year-old male patient was first treated for gastroenteritis for 7 days. two months later, he was admitted to the hospital and the aneurysm was diagnosed by three-dimensional computed tomography. the aneurysm was excised and the restoration was done using a 16-mm dacron straight graft. the cultivation of the excised an ...	2009	19198993
dietary supplementation of mannan oligosaccharide improves the immune responses and survival of marron, cherax tenuimanus (smith, 1912) when challenged with different stressors.	three trials were conducted to determine the effects of mannan oligosaccharide (bio-mos ) on the immune responses of marron. in the first trial marron were challenged with vibrio mimicus infection, in the second with nh3 exposure and in the third, the marron were exposed to air during a simulated live transportation trial. for v. mimicus infection and live transportation trials, marron (10.44 +/- 0.20 g and 4.44 +/- 0.20 g initial weights, respectively) were fed three different diets containing ...	2009	19539033
rna colony blot hybridization method for enumeration of culturable vibrio cholerae and vibrio mimicus bacteria.	a species-specific rna colony blot hybridization protocol was developed for enumeration of culturable vibrio cholerae and vibrio mimicus bacteria in environmental water samples. bacterial colonies on selective or nonselective plates were lysed by sodium dodecyl sulfate, and the lysates were immobilized on nylon membranes. a fluorescently labeled oligonucleotide probe targeting a phylogenetic signature sequence of 16s rrna of v. cholerae and v. mimicus was hybridized to rrna molecules immobilized ...	2009	19561182
simultaneous differential detection of human pathogenic and nonpathogenic vibrio species using a multiplex pcr based on gyrb and pnta genes.	to develop a multiplex pcr targeting the gyrb and pnta genes for vibrio species differentiation.	2010	19891709
validation of a method for the detection of five species, serogroups, biotypes and virulence factors of vibrio by multiplex pcr in fish and seafood.	in this work a sequential multiplex pcr system was designed and validated for the detection of most frequent foodborne pathogen vibrio species in fish and seafood (vibrio cholerae, vibrio parahaemolyticus, vibrio vulnificus, vibrio alginoliticus and vibrio mimicus). the method proposed functions in a hierarchical way, being composed of an end-point multiplex pcr to detect the presence of dna belonging to the studied species, followed by multiplex pcr and fragment analysis allowing the viability ...	2010	19913702
[development of taqman real-time pcr in detection of aeromonas hydrophila].	to develop a taqman real-time pcr for the detection of aeromonas hydrophila.	2009	19954074
development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of the tdh and trh genes of vibrio parahaemolyticus and related vibrio species.	thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) are the major virulence determinants of vibrio parahaemolyticus. trh is further differentiated into trh1 and trh2 on the basis of genetic and phenotypic differences. we developed a novel and highly specific loop-mediated isothermal amplification (lamp) assay for sensitive and rapid detection of the tdh, trh1, and trh2 genes of v. parahaemolyticus. the lamp assay was designed for both combined and individual detection of the tdh, ...	2010	19966027
rapid identification and characterization of vibrio species using whole-cell maldi-tof mass spectrometry.	vibrio identification by means of traditional microbiological methods is time consuming because of the many biochemical tests that have to be performed to distinguish closely related species. this work aimed at evaluating the use of maldi-tof mass spectrometry for the rapid identification of vibrio (v.) spp. as an advantageous application to rapidly discriminate the most important vibrio spp. and distinguish vibrio spp. from closely related bacterial species like photobacterium damselae and grim ...	2010	20059616
occurrence of the vibrio cholerae seventh pandemic vsp-i island and a new variant.	using comparative genomics, we identified a new variant of the vibrio seventh pandemic island-i (vsp-i). results of polymerase chain reaction (pcr) screening for both known vsp-i variants indicate that the novel variant is present only in non-o1/non-o139 strains of v. cholerae and vibrio mimicus. comparative genomics revealed little sequence divergence in the seventh pandemic vsp-i; however, a second insertion site located on the smaller chromosome was identified. although the seventh pandemic v ...	2010	20141327
[simultaneous detection of vibrio cholerae o1 and o139 by real-time quantitative pcr].	targeting the specific o-antigen gene cluster, a taq-man real-time fluorescence pcr assay was developed to detect 01 and o139 vibrio cholera concurrently and avoid frequently occurred false positives.	2010	20364601
in vitro antibacterial, cytotoxic and antioxidant activities of plant nephelium longan.	the petroleum ether, chloroform and ethyl acetate fractions of ethanol extract of leaf and stem from the plant nephelium longan (fam-sapindaceae) was subjected to antioxidant, antibacterial and cytotoxic activity. all the fractions showed potent antioxidant activity, of which the ethyl acetate and chloroform fraction of leaf demonstrated the strongest antioxidant activity with the ic50 value of 44.28 and 44.31 microg ml(-1), respectively. the petroleum ether extracts (500 microg disc(-1)) of lea ...	2010	20415149
identification of non-coding rnas in environmental vibrios.	the discovery of non-coding rna (ncrna) has been mainly limited to laboratory model systems and human pathogenic bacteria. in this study, we begin to explore the ncrna diversity in four recently sequenced environmental vibrio species (vibrio alginolyticus 40b, vibrio communis 1da3, vibrio mimicus vm573 and vibrio campbellii baa-1116) by performing in silico searches using infernal and rfam for the identification of putative ncrna-encoding genes. this search method resulted in the identification ...	2010	20447992
interaction between vibrio mimicus and acanthamoeba castellanii.	vibrio mimicus is a gram-negative bacterium, which causes gastroenteritis and is closely related to vibrio cholerae. the environmental reservoir of this bacterium is far from defined. acanthamoeba as well as vibrio species are found in diverse aquatic environments. the present study was aimed to investigate the ability of a. castellanii to host v. mimicus, the role of bacterial protease on interaction with a. castellanii and to disclose the ability of cysts to protect intracellular v. mimicus. c ...	2010	20454692
discovery of novel vibrio cholerae vsp-ii genomic islands using comparative genomic analysis.	this report describes vibrio seventh pandemic island ii (vsp-ii) and three novel variants revealed by comparative genomics of 23 vibrio cholerae strains and their presence among a large and diverse collection of v. cholerae isolates. three vsp-ii variants were reported previously and our results demonstrate the presence of three novel vsp-ii in clinical and environmental v. cholerae marked by major deletions and genetic rearrangements. a new vsp-ii cluster was found in the seventh pandemic v. ch ...	2010	20528940
dietary medicinal plant extracts improve growth, immune activity and survival of tilapia oreochromis mossambicus.	the effects of supplementing diets with acetone extract (1% w/w) from four medicinal plants (bermuda grass cynodon dactylon, h(1), beal aegle marmelos, h(2), winter cherry withania somnifera, h(3) and ginger zingiber officinale, h(4)) on growth, the non-specific immune response and ability to resist pathogen infection in tilapia oreochromis mossambicus were assessed. in addition, the antimicrobial properties of the extract were assessed against vibrio alginolyticus, vibrioparahaemolyticus, vibri ...	2009	20735646
notes from the field: vibrio mimicus infection from consuming crayfish --- spokane, washington, june 2010.	on june 24, 2010, the spokane (washington) regional health district (srhd) was notified of two hospitalized patients under intensive care with severe dehydration whose stool specimens yielded vibrio mimicus. cdc was asked to assist with the environmental and epidemiologic investigation. investigators learned that both persons had consumed crayfish on june 20, 2010. the previous day, live crayfish obtained from an online seafood company had been boiled and served warm at a party. the chef reporte ...	2010	21030943
comparative genomics of clinical and environmental vibrio mimicus.	whether vibrio mimicus is a variant of vibrio cholerae or a separate species has been the subject of taxonomic controversy. a genomic analysis was undertaken to resolve the issue. the genomes of v. mimicus mb451, a clinical isolate, and vm223, an environmental isolate, comprise ca. 4,347,971 and 4,313,453 bp and encode 3,802 and 3,290 orfs, respectively. as in other vibrios, chromosome i (c-i) predominantly contains genes necessary for growth and viability, whereas chromosome ii (c-ii) bears gen ...	2010	21078967
presence of genes for type iii secretion system 2 in vibrio mimicus strains.	vibrios, which include more than 100 species, are ubiquitous in marine and estuarine environments, and several of them e.g. vibrio cholerae, v. parahaemolyticus, v. vulnificus and v. mimicus, are pathogens for humans. pathogenic v. parahaemolyticus strains possess two sets of genes for type iii secretion system (t3ss), t3ss1 and t3ss2. the latter are critical for virulence of the organism and be classified into two distinct phylogroups, t3ss2α and t3ss2β, which are reportedly also found in patho ...	2010	21110901
identification and characterization of a vibrio mimicus gene encoding the heme/hemoglobin receptor.	the present authors have previously reported that vibrio mimicus expresses 77-kda and 80-kda outer membrane proteins in response to iron-limited conditions, and that the 77-kda protein serves as the receptor for ferriaerobactin. in this study, it was found that v. mimicus can use heme and hemoglobin as iron sources. furta was then applied to v. mimicus 7pt to obtain candidate gene fragments involved in utilization of heme and hemoglobin. one furta-positive clone was shown to contain a partial ge ...	2010	21118298
development of a single base extension-tag microarray for the detection of pathogenic vibrio species in seafood.	in this study, a single base extension-tag array on glass slides (sbe-tags) microarray was established to detect the seven leading seafood-borne pathogens, including vibrio parahaemolyticus, vibrio cholerae, vibrio vulnificus, vibrio mimicus, vibrio alginolyticus, vibrio anguillarum, and vibrio harveyi. three multiplex pcr assays were developed to specifically target the following species with individual gene markers, which are aads, tdh, and trh for v. parahaemolyticus; col, toxr, and vvh for v ...	2010	21161212
[identification of vibrio mimicus bacteriophages].	lysogeny was studied in vibrio mimicus; the indicator v. cholerae el tor strain was selected to identify phages. new v. mimicus phages were obtained and identified, which had a morphological similarity and an antigen affinity for morphological group i cholerae phages. phage differentiation revealed that morphological group i v. mimicus phages showed certain differences manifested as their lytic activity against v. cholerae strain 1322-69 of serovar 37 while this property was absent in cholerae p ...	2011	21574461
design and validation of a novel multiplex real-time pcr assay for vibrio pathogen detection.	three species--vibrio cholerae, vibrio parahaemolyticus, and vibrio vulnificus--account for the majority of vibrio infections in humans. rapid and accurate identification of vibrio species has been problematic because phenotypic characteristics are variable within species. additionally, biochemical identification and confirmation require 2 or more days to complete. rapid and sensitive molecular techniques for the detection of vibrio pathogens would be useful for the surveillance and management o ...	2011	21669071
genome sequencing reveals unique mutations in characteristic metabolic pathways and the transfer of virulence genes between v. mimicus and v. cholerae.	vibrio mimicus, the species most similar to v. cholerae, is a microbe present in the natural environmental and sometimes causes diarrhea and internal infections in humans. it shows similar phenotypes to v. cholerae but differs in some biochemical characteristics. the molecular mechanisms underlying the differences in biochemical metabolism between v. mimicus and v. cholerae are currently unclear. several v. mimicus isolates have been found that carry cholera toxin genes (ctxab) and cause cholera ...	2011	21731695
erratum: variation in epitopes of the b subunit of vibrio cholerae non-o1 and vibrio mimicus cholera toxins.		1990	22049941
Detection and characterization of pathogenic vibrios in shellfish by a Ligation Detection Reaction-Universal Array approach.	Vibrios are a group of major foodborne pathogens widely distributed in marine environment. Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus are the pathogenic species of Vibrio that pose the greatest threat to human health. However, other vibrios, e.g. Vibrio alginolyticus, Vibrio mimicus and Grimontia hollisae, apparently less relevant in the group of foodborne pathogens, have been sporadically found in outbreaks. For seafood safety and economic purposes, a rapid and powerful met ...	2011	22177227
structural variation of the superintegron in the toxigenic vibrio cholerae o1 el tor.	to understand the genetic structures and variations of the superintegron (si) in vibrio cholerae isolated in the seventh cholera pandemic.	2011	22365393
vibrio mimicus infection associated with crayfish consumption, spokane, washington, 2010.	we report a cluster of severe diarrheal disease caused by vibrio mimicus infection among four persons who had consumed leftover crayfish the day after a private crayfish boil. gastrointestinal illness caused by vibrio mimicus has not been reported previously in washington state. three cases were laboratory confirmed by stool culture; using pcr, isolates were found to have ctx genes that encode cholera toxin (ct). two of the cases were hospitalized under intensive care with a cholera-like illness ...	2012	22488068
examining the fish microbiome: vertebrate-derived bacteria as an environmental niche for the discovery of unique marine natural products.	historically, marine invertebrates have been a prolific source of unique natural products, with a diverse array of biological activities. recent studies of invertebrate-associated microbial communities are revealing microorganisms as the true producers of many of these compounds. inspired by the human microbiome project, which has highlighted the human intestine as a unique microenvironment in terms of microbial diversity, we elected to examine the bacterial communities of fish intestines (which ...	2012	22574119