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rapid identification and characterization of vibrio species using whole-cell maldi-tof mass spectrometry.vibrio identification by means of traditional microbiological methods is time consuming because of the many biochemical tests that have to be performed to distinguish closely related species. this work aimed at evaluating the use of maldi-tof mass spectrometry for the rapid identification of vibrio (v.) spp. as an advantageous application to rapidly discriminate the most important vibrio spp. and distinguish vibrio spp. from closely related bacterial species like photobacterium damselae and grim ...201020059616
optimization of excimer-forming two-probe nucleic acid hybridization method with pyrene as a fluorophore.a previously presented homogeneous assay method, named the excimer-forming two-probe nucleic acid hybridization (etph) method, is based on specific excimer formation between two pyrenes attached at the neighboring terminals of two sequential probe oligonucleotides complementary to a single target. in this study, we investigated assay conditions and optimal molecular design of probes for intense excimer emission using a pyrenemethyliodoacetamide-introduced 16mer probe, a pyrene butanoic acid-intr ...19989826766
distribution of virulence-associated genes in vibrio mimicus isolates from clinical and environmental origins.distribution of virulence-associated genes in vibrio mimicus was studied including the toxin genes ctxa, tdh, st and vmh and the genes necessary for regulation of toxin production, toxr, toxs, toxt, tcpa and tcpp. approximately half of clinical v. mimicus isolates possessed one or more genes encoding v. cholerae enterotoxic factors such as ctxa, tdh and st. all of the clinical and environmental isolates possessed vmh encoding v. mimicus hemolysin (vmh). the ctxa encoding cholera toxin was detect ...200415272201
in vitro antibacterial, cytotoxic and antioxidant activities of plant nephelium longan.the petroleum ether, chloroform and ethyl acetate fractions of ethanol extract of leaf and stem from the plant nephelium longan (fam-sapindaceae) was subjected to antioxidant, antibacterial and cytotoxic activity. all the fractions showed potent antioxidant activity, of which the ethyl acetate and chloroform fraction of leaf demonstrated the strongest antioxidant activity with the ic50 value of 44.28 and 44.31 microg ml(-1), respectively. the petroleum ether extracts (500 microg disc(-1)) of lea ...201020415149
identification of non-coding rnas in environmental vibrios.the discovery of non-coding rna (ncrna) has been mainly limited to laboratory model systems and human pathogenic bacteria. in this study, we begin to explore the ncrna diversity in four recently sequenced environmental vibrio species (vibrio alginolyticus 40b, vibrio communis 1da3, vibrio mimicus vm573 and vibrio campbellii baa-1116) by performing in silico searches using infernal and rfam for the identification of putative ncrna-encoding genes. this search method resulted in the identification ...201020447992
evolutionary genetic analysis of the emergence of epidemic vibrio cholerae isolates on the basis of comparative nucleotide sequence analysis and multilocus virulence gene profiles.vibrio cholerae, the causative agent of cholera, is a natural inhabitant of the aquatic ecosystem. we examined a unique collection of v. cholerae clinical and environmental isolates of widespread geographic distribution recovered over a 60-year period to determine their evolutionary genetic relationships based on analysis of two housekeeping genes, malate dehydrogenase (mdh) and a chaperonin (groel). in addition, the phylogenetic distribution of 12 regions associated with virulence was determine ...200415472325
a hemolysin of vibrio mimicus (vmh) stimulates cells to produce atp and cyclic amp which appear to be secretory mediators.the hemolysin of vibrio mimicus(vmh) is a pore-forming toxin with both enterotoxic and hemolytic activity. the hemolysis by vmh is induced by creation of pores in the membrane of erythrocyte; however, the mechanism for the enterotoxic action of vmh has remained unclear. in order to clarify the mechanism, we incubated t84 cells (a human colon carcinoma cell line) with vmh and found that the levels of atp and cyclic amp of culture medium increased after exposure of the cells to vmh. subsequently, ...200515665456
seasonality of chesapeake bay bacterioplankton species.bacteria, gamma-subclass of proteobacteria, vibrio-photobacterium, vibrio vulnificus, vibrio cholerae-vibrio mimicus, and vibrio cincinnatiensis in water samples collected from the choptank river in chesapeake bay from 15 april to 16 december 1996 were enumerated using a fluorescent oligonucleotide direct-counting (fodc) procedure. fodc results obtained using a bacteria taxon-specific probe ranged from one-third the number of to the same number as that obtained by the acridine orange direct coun ...200212406742
[pathogenic factors of vibrios with special emphasis on vibrio vulnificus].bacteria of the genus vibrio are normal habitants of the aquatic environment and play roles for biocontrole of aquatic ecosystem, but some species are believed to be human pathogens. these species can be classified into two groups according to the types of diseases they cause: the gastrointestinal infections and the extraintestinal infections. the pathogenic species produce various pathogenic factors including enterotoxin, hemolysin, cytotoxin, protease, siderophore, adhesive factor, and hemaggl ...200515997210
use of reca as an alternative phylogenetic marker in the family vibrionaceae.this study analysed the usefulness of reca gene sequences as an alternative phylogenetic and/or identification marker for vibrios. the reca sequences suggest that the genus vibrio is polyphyletic. the high heterogeneity observed within vibrios was congruent with former polyphasic taxonomic studies on this group. photobacterium species clustered together and apparently nested within vibrios, while grimontia hollisae was apart from other vibrios. within the vibrios, vibrio cholerae and vibrio mimi ...200415143042
molecular evolution of vibrio pathogenicity island-2 (vpi-2): mosaic structure among vibrio cholerae and vibrio mimicus natural isolates.vibrio cholerae is a gram-negative rod that inhabits the aquatic environment and is the aetiological agent of cholera, a disease that is endemic in much of southern asia. the 57.3 kb vibrio pathogenicity island-2 (vpi-2) is confined predominantly to toxigenic v. cholerae o1 and o139 serogroup isolates and encodes 52 orfs (vc1758 to vc1809), which include homologues of an integrase (vc1758), a restriction modification system, a sialic acid metabolism gene cluster (vc1773-vc1783), a neuraminidase ...200515632448
identification and characterization of a vibrio mimicus gene encoding the heme/hemoglobin receptor.the present authors have previously reported that vibrio mimicus expresses 77-kda and 80-kda outer membrane proteins in response to iron-limited conditions, and that the 77-kda protein serves as the receptor for ferriaerobactin. in this study, it was found that v. mimicus can use heme and hemoglobin as iron sources. furta was then applied to v. mimicus 7pt to obtain candidate gene fragments involved in utilization of heme and hemoglobin. one furta-positive clone was shown to contain a partial ge ...201021118298
improvement of innate immune responses and defense activity in mitten crab (eriocheir sinensis) by oral administration of beta-glucan.the beta-glucan (bg), extracted from saccharomyces cerevisiae cell wall, was orally administrated to the mitten crab (eriocheir sinensis) at 0, 1, 5 and 10 g bg/kg diet for 28 days, followed by a challenge with vibrio mimicus by intramuscular injection. growth, phenoloxidase, superoxide dismutase, acid phosphatase and alkaline phosphatase activity were monitored after 14 and 28 days. the results showed an immunomodulatory effect and protection against v. mimicus by dietary supplementation of bg. ...200818568402
development of a hexaplex pcr assay for rapid detection of virulence and regulatory genes in vibrio cholerae and vibrio mimicus.we have developed a hexaplex pcr assay for rapid detection of the virulence and regulatory genes for cholera toxin enzymatic subunit a (ctxa), zonula occludens toxin (zot), accessory cholera enterotoxin (ace), toxin-coregulated pilus (tcpa), outer membrane protein (ompu), and central regulatory protein toxr (toxr) in vibrio cholerae and vibrio mimicus. this hexaplex pcr proved successful in screening pathogenic-toxigenic and nonpathogenic-nontoxigenic v. cholerae and v. mimicus strains from both ...200212409420
haemolysin produced by vibrio mimicus activates two cl- secretory pathways in cultured intestinal-like caco-2 cells.haemolysin (vmh) is a virulent factor produced by vibrio mimicus, a human pathogen that causes diarrhoea. as intestinal epithelial cells are the primary targets of haemolysin, we investigated its effects on ion transport in human colonic epithelial caco-2 cells. vmh increased the cellular short circuit current (isc), used to estimated ion fluxes, and 125i efflux of the cells. the vmh-induced increases in isc and 125i efflux were suppressed by depleting ca2+ from the medium or by pretreating the ...200717026482
identification of vibrio isolates by a multiplex pcr assay and rpob sequence determination.vibrio, a diverse genus of aquatic bacteria, currently includes 72 species, 12 of which occur in human clinical samples. of these 12, three species--vibrio cholerae, vibrio parahaemolyticus, and vibrio vulnificus-account for the majority of vibrio infections in humans. rapid and accurate identification of vibrio species has been problematic because phenotypic characteristics are variable within species and biochemical identification requires 2 or more days to complete. to facilitate the identifi ...200717093013
Detection and characterization of pathogenic vibrios in shellfish by a Ligation Detection Reaction-Universal Array approach.Vibrios are a group of major foodborne pathogens widely distributed in marine environment. Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus are the pathogenic species of Vibrio that pose the greatest threat to human health. However, other vibrios, e.g. Vibrio alginolyticus, Vibrio mimicus and Grimontia hollisae, apparently less relevant in the group of foodborne pathogens, have been sporadically found in outbreaks. For seafood safety and economic purposes, a rapid and powerful met ...201122177227
proteolytic activation of vibrio mimicus (vm) major outer membrane protein haemagglutinin (ha) with vm-ha/protease: implication for understanding bacterial adherence.vibrio mimicus (vm) haemagglutinins (has), such as an extracellular ha/protease (vm-ha/protease) and a major outer membrane protein-ha (vm-ompha), have been recognized as the putative adherence factors for the bacterium. however, the mechanism by which has coordinate the adherence function of the bacterium remains as yet unknown. we report herein the positive interaction between vm-ha/protease and vm-ompha resulting in significant enhancement of the haemagglutinating ability. in this interaction ...200617116978
rapid and specific identification of 5 human pathogenic vibrio species by multiplex polymerase chain reaction targeted to dnaj gene.a multiplex polymerase chain reaction (pcr) method, specifically designed for application in routine diagnostic laboratories, was developed for identifying 5 human pathogen vibrio species: vibrio cholerae, vibrio parahaemolyticus, vibrio vulnificus, vibrio mimicus, and vibrio alginolyticus. this assay directed toward the dnaj gene was tested on a total of 355 strains representing 13 vibrio species and 17 non-vibrio species. specific pcr fragments were produced in isolates belonging to the 5 targ ...200717614235
comparative genomics of clinical and environmental vibrio mimicus.whether vibrio mimicus is a variant of vibrio cholerae or a separate species has been the subject of taxonomic controversy. a genomic analysis was undertaken to resolve the issue. the genomes of v. mimicus mb451, a clinical isolate, and vm223, an environmental isolate, comprise ca. 4,347,971 and 4,313,453 bp and encode 3,802 and 3,290 orfs, respectively. as in other vibrios, chromosome i (c-i) predominantly contains genes necessary for growth and viability, whereas chromosome ii (c-ii) bears gen ...201021078967
mycotic abdominal aortic aneurysm infected by vibrio mimicus: report of a case.this report describes a case of a mycotic aneurysm of the infrarenal abdominal aorta infected by vibrio mimicus, which is the only such case ever reported in the literature. the 80-year-old male patient was first treated for gastroenteritis for 7 days. two months later, he was admitted to the hospital and the aneurysm was diagnosed by three-dimensional computed tomography. the aneurysm was excised and the restoration was done using a 16-mm dacron straight graft. the cultivation of the excised an ...200919198993
mechanism of action of beta-blockers in hypertension. 200114643
statistical analysis of epidemiological data from a chromium chemical manufacturing plant.a statistical analysis was performed on reported bronchogenic carcinoma incidence in a group of chromium chemical manufacturing workers over the period 1929 to 1977. the purpose was to determine if post-1950 process improvements corresponded to favorable trends in morbidity. because the paucity of data on the population at risk prevented meaningful calculations of smr's, a recently developed method called "probability window analysis" was used to evaluate for statistically valid changes in the " ...2003430272
pelvic displacement of a cardiac pacemaker following trauma. a case report.the rate migration of an implanted cardiac pacemaker from the subrectus area anterior to the peritoneum to the pelvis following a motor vehicle accident is reported. discovery of dislodgement was made by observing a marked increase of pacemaker impulse amplitude in the three standard limb leads as measured on an oscilloscope.2003152472
structural variation of the superintegron in the toxigenic vibrio cholerae o1 el tor.to understand the genetic structures and variations of the superintegron (si) in vibrio cholerae isolated in the seventh cholera pandemic.201122365393
annual summary 1984. reported morbidity and mortality in the united states. 20113092006
evaluation of aerosolized triamcinolone acetonide as a replacement for aerosolized beclomethasone dipropionate.substitution of aerosolized triamcinolone acetonide for aerosolized beclomethasone dipropionate in treatment of 30 chronic asthmatics resulted in subjective improvement in all patients. there was reduction in requirement of systemic steroids and inhaled beta adrenergics. no adrenal suppression or oropharyngeal candidiasis occurred after replacing beclomethasone dipropionate with triamcinolone acetonide.20123528120
software printout. 20154542580
[lysis of the femoral neck and coxa vara (author's transl)]. 20154588540
behaviour of carbonic anhydrase in the uterus of hens during moult. 20164958241
computer-aided molecular modelling: research study or research tool?developments in computational methods and equipment have produced a new type of research chemist, who prefers to calculate properties as well as measure them, either to gain a better understanding of microscopic molecular behaviour per se, or to guide a broader scientific study using a so-called 'rational' approach. while there is good reason to believe that significant results can be obtained this way, it is clear that only some of the 'tools of the trade' are sufficiently robust to present to ...20113236047
vibrio porteresiae sp. nov., a diazotrophic bacterium isolated from a mangrove-associated wild rice (porteresia coarctata tateoka).two facultatively anaerobic, nitrogen-fixing bacteria (strains mssrf30(t) and mssrf31) were isolated from a mangrove-associated wild rice (porteresia coarctata tateoka). these strains were determined to be nitrogen-fixers using the acetylene reduction assay and by pcr detection of a nifh gene amplicon. phylogenetic analyses based on 16s rrna gene sequences indicated that the novel strains were most closely related to vibrio fluvialis lmg 7894(t) (96.8 % gene sequence similarity), vibrio furnissi ...200818599703
modulation of vibrio mimicus hemolysin through limited proteolysis by an endogenous metalloprotease.vibrio mimicus is a causative agent of human gastroenteritis and food poisoning, and this species produces an enterotoxic hemolysin (v. mimicus hemolysin) as a virulence determinant. vibrio mimicus hemolysin is secreted as an 80 kda precursor, which is later converted to the 66 kda mature toxin through removal of an n-terminal propeptide via cleavage of the arg151-ser152 bond. in this article, we investigate the role of the endogenous metalloprotease (v. mimicus protease) in the maturation of v. ...200919143841
reverse genetics approaches for cloning ril-1, a major locus involved in susceptibility to leukemia. 20123416638
the feasibility of an assistant to the physician. 20144152241
[association of an ossicle malformation and a congenital preauricular fistula]. 2003142810
[x-ray computed tomography in venous pathology].after presenting the tomodensitometric semiology of venous thromboses in various territories, the authors define its indications. it is essentially in deep territories, head, chest and abdomen that the ct-scan is used to study and diagnose unrecognized thromboses as well as determine the venous repercussion of various neighboring ailments. the respective place of the ct-scan, ultrasonography and various phlebographies is analyzed.20113043474
development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of the tdh and trh genes of vibrio parahaemolyticus and related vibrio species.thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) are the major virulence determinants of vibrio parahaemolyticus. trh is further differentiated into trh1 and trh2 on the basis of genetic and phenotypic differences. we developed a novel and highly specific loop-mediated isothermal amplification (lamp) assay for sensitive and rapid detection of the tdh, trh1, and trh2 genes of v. parahaemolyticus. the lamp assay was designed for both combined and individual detection of the tdh, ...201019966027
overdose with baclofen. 20143945841
occurrence of the vibrio cholerae seventh pandemic vsp-i island and a new variant.using comparative genomics, we identified a new variant of the vibrio seventh pandemic island-i (vsp-i). results of polymerase chain reaction (pcr) screening for both known vsp-i variants indicate that the novel variant is present only in non-o1/non-o139 strains of v. cholerae and vibrio mimicus. comparative genomics revealed little sequence divergence in the seventh pandemic vsp-i; however, a second insertion site located on the smaller chromosome was identified. although the seventh pandemic v ...201020141327
presence of genes for type iii secretion system 2 in vibrio mimicus strains.vibrios, which include more than 100 species, are ubiquitous in marine and estuarine environments, and several of them e.g. vibrio cholerae, v. parahaemolyticus, v. vulnificus and v. mimicus, are pathogens for humans. pathogenic v. parahaemolyticus strains possess two sets of genes for type iii secretion system (t3ss), t3ss1 and t3ss2. the latter are critical for virulence of the organism and be classified into two distinct phylogroups, t3ss2α and t3ss2β, which are reportedly also found in patho ...201021110901
self-evaluation by first-year medical students in a clinical science programme. 20164771337
the mesencephalic exterolateral posterior nucleus of the mormyrid fish bryenomyrus niger: efferent connections studied by the hrp method. 199991414
perinatal mortality. 200193220
[present-day state and perspectives of development of psychotherapy in poland]. 2004515249
[massive lower gastrointestinal hemorrhage of diverticular origin. diagnostic and therapeutic problems]. 20051082809
[70 years nursing school innsbruck]. 20092694061
discovery of novel vibrio cholerae vsp-ii genomic islands using comparative genomic analysis.this report describes vibrio seventh pandemic island ii (vsp-ii) and three novel variants revealed by comparative genomics of 23 vibrio cholerae strains and their presence among a large and diverse collection of v. cholerae isolates. three vsp-ii variants were reported previously and our results demonstrate the presence of three novel vsp-ii in clinical and environmental v. cholerae marked by major deletions and genetic rearrangements. a new vsp-ii cluster was found in the seventh pandemic v. ch ...201020528940
genome sequencing reveals unique mutations in characteristic metabolic pathways and the transfer of virulence genes between v. mimicus and v. cholerae.vibrio mimicus, the species most similar to v. cholerae, is a microbe present in the natural environmental and sometimes causes diarrhea and internal infections in humans. it shows similar phenotypes to v. cholerae but differs in some biochemical characteristics. the molecular mechanisms underlying the differences in biochemical metabolism between v. mimicus and v. cholerae are currently unclear. several v. mimicus isolates have been found that carry cholera toxin genes (ctxab) and cause cholera ...201121731695
thyroglobulin and 131i uptake of remaining tissue in patients with differentiated carcinoma after thyroidectomy.in 158 thyroidectomized patients with well-differentiated non-metastatic thyroid cancer the results of serum thyroglobulin (tg) determination and 131i uptake values established immediately before radioiodine treatment (19 days after surgery) were compared. in 113 patients (72%) tg was above 6 ng ml-1 (lower limit of detection) and 131i uptake exceeded 2%. in 11 patients (7%) tg was undetectable and 131i uptake less than 2%. in these cases a radioiodine treatment was not performed; the thyroid ab ...20123399222
galvanic skin responses (gsr) and plethysmographic changes induced by chlorimipramine, imipramine and placebo. 20144209521
erratum: variation in epitopes of the b subunit of vibrio cholerae non-o1 and vibrio mimicus cholera toxins. 199022049941
[are reforms of dental education necessary?]. 20154510183
hypersomatotropism and acanthosis nigricans in two brothers. 20164843205
synthesis and antineoplastic effects of furo[3,4-d]pyrimidine derivatives.furo[3,4-d]pyrimidine was obtained in the reaction of 2-phenyl-4-phenylamino-6-methyl-5-pyrimidinecarboxylic acid with socl2. this compound, heated with aliphatic amines, yielded mono- and diamino-derivatives. some of the obtained compounds exhibited a potent antineoplastic activity.20113237569
fetal heart rate during a maternal grand mal epileptic seizure.although maternal ingestion of antiepileptic drugs is strongly suspected of causing congenital defects, particularly oral clefts, the effect of epilepsy itself or a combined effect of drug intake and epilepsy have not been excluded as etiological factors. very little is known about fetal oxygenation during a maternal grand mal epileptic seizure. we describe two cases in which fetal heart rate was recorded during a maternal epileptic seizure during labor. the first fetus became clearly asphyctic ...1998106102
darkroom diseases and how to combat them. 20113112384
anticholinergic drugs in the treatment of airways disease. 20102861843
[disturbances in phosphate metabolism during parenteral alimentation. hypophosphatemic syndrome]. 199824396
identification of vibrio cholerae and vibrio mimicus by multilocus sequence analysis (mlsa).vibrio cholerae and vibrio mimicus have similar phenotypes and genomes making rapid differentiation of these two species difficult. the first standard multilocus sequence analysis (mlsa) scheme for the identification of these species is described. a collection of 45 representative isolates from different geographical regions and hosts was examined using segments of the housekeeping genes pyrh, reca and rpoa. overall, the closest phylogenetic neighbours of these species were vibrio furnissii and ...200818319466
notes from the field: vibrio mimicus infection from consuming crayfish --- spokane, washington, june 2010.on june 24, 2010, the spokane (washington) regional health district (srhd) was notified of two hospitalized patients under intensive care with severe dehydration whose stool specimens yielded vibrio mimicus. cdc was asked to assist with the environmental and epidemiologic investigation. investigators learned that both persons had consumed crayfish on june 20, 2010. the previous day, live crayfish obtained from an online seafood company had been boiled and served warm at a party. the chef reporte ...201021030943
a simple centrifuge column for desalting protein solutions. 2005543535
health checks on patients 75 years and over in nottinghamshire after the new gp contract.to investigate annual health checks for patients of 75 years and over required by the 1990 contract for general practitioners.20061393076
[identification of vibrio mimicus bacteriophages].lysogeny was studied in vibrio mimicus; the indicator v. cholerae el tor strain was selected to identify phages. new v. mimicus phages were obtained and identified, which had a morphological similarity and an antigen affinity for morphological group i cholerae phages. phage differentiation revealed that morphological group i v. mimicus phages showed certain differences manifested as their lytic activity against v. cholerae strain 1322-69 of serovar 37 while this property was absent in cholerae p ...201121574461
a methoxylated fatty acid isolated from the brown seaweed ishige okamurae inhibits bacterial phospholipase a2.a methoxylated fatty acid that inhibits phospholipase a(2) (pla(2); ec 3.1.1.4) was purified from the brown seaweed ishige okamurae. approximately 8.1 mg of the inhibitory compound, 7-methoxy-9-methylhexadeca-4,8-dienoic acid, was isolated from 1 kg of i. okamurae powder. recombinant pla(2) derived from the pathogenic bacterium vibrio mimicus was used as the target enzyme. the methoxylated fatty acid compound competitively inhibited pla(2) with a ki value of 3.9 microg/ml. the concentrations req ...200818570268
a randomized trial of anticoagulation with warfarin and of alternating chemotherapy in extensive small-cell lung cancer by the cancer and leukemia group b.the cancer and leukemia group b (calgb) conducted a prospective randomized trial to evaluate the role of warfarin and alternating chemotherapy in extensive small-cell lung cancer (sccl). after stratification for sex and performance status, patients were randomly assigned to receive chemotherapy with methotrexate, doxorubicin (adriamycin; adria laboratories, columbus, oh), cyclophosphamide, and lomustine (ccnu) (macc), or macc plus warfarin (macc + w), or mitomycin, etoposide, cisplatin, and hexa ...20082547030
oesophageal pain exacerbated by propranolol. 20113113570
change of blood pressure over five years in childhood and adolescence.we compared blood pressure (bp) values, measured at a five year interval, in 2,777 boys and girls aged 4-18 years. bp values were converted into height and sex adjusted standard deviation unit scores. correlation coefficients were significant but low for systolic bp score: 0.19 in boys and 0.15 in girls, and were even lower for diastolic bp score. these correlations are weaker than those observed for overall bp values (boys: 0.33, girls: 0.23), weight (0.82, 0.75) and height (0.87, 0.80). the pr ...20133757275
an incidence of large foodborne outbreak associated with vibrio mimicus. 200918925423
diagnosis and treatment of chalazia.a patient with recurrent chalazia is described. this patient also had acne rosacea. the clinical and histologic characteristics of chalazia are described. in addition, therapy for chalazia is presented, with emphasis being given to conservative therapy.20133734179
[acoustic indicator of membrane potential levels]. 20133609385
the relationship between intracellular and extracellular potassium in normal and malnourished subjects as studied in leukocytes.leukocyte potassium has been measured in normal subjects and in malnourished children with and without edema both on admission and during recovery. in nonedematous malnourished children the potassium content was low (328 mmol/kg dry solids) and took 2-3 weeks of rehabilitation to return to normal (374 mmol/kg dry solids). leukocytes from edematous children had normal intracellular potassium values. however, 5 days on a maintenance diet reduced the intracellular potassium in the children with kwa ...199999717
[effectiveness of the comprehensive treatment by rhonidase electrophoresis and tbilisi sulfide baths of elderly patients suffering from osteoarthrosis deformans]. 2003154769
[use of audiovisual equipment in the department of dentistry in japanese universities (2)]. 200195111
male transsexualism in england: sixteen cases with surgical intervention.the syndrome of transsexualism is now regarded as a dinstinct entity separate from transvestism and homosexuality (benjamin, 1966; green and money, 1969). it refers to those patients who since childhood have been convinced of their wrong sex assignment and who wish to attain the sexual characteristics of the opposite sex and to live as a member of that sex. the present report is an initial one referring to the first group of transsexual patients studied at a postgraduate teaching hospital by a m ...20051169047
dietary supplementation of mannan oligosaccharide improves the immune responses and survival of marron, cherax tenuimanus (smith, 1912) when challenged with different stressors.three trials were conducted to determine the effects of mannan oligosaccharide (bio-mos ) on the immune responses of marron. in the first trial marron were challenged with vibrio mimicus infection, in the second with nh3 exposure and in the third, the marron were exposed to air during a simulated live transportation trial. for v. mimicus infection and live transportation trials, marron (10.44 +/- 0.20 g and 4.44 +/- 0.20 g initial weights, respectively) were fed three different diets containing ...200919539033
[organization of the regular schedules of forensic-medical experts at a bureau of forensic medical examinations]. 20144071589
design and validation of a novel multiplex real-time pcr assay for vibrio pathogen detection.three species--vibrio cholerae, vibrio parahaemolyticus, and vibrio vulnificus--account for the majority of vibrio infections in humans. rapid and accurate identification of vibrio species has been problematic because phenotypic characteristics are variable within species. additionally, biochemical identification and confirmation require 2 or more days to complete. rapid and sensitive molecular techniques for the detection of vibrio pathogens would be useful for the surveillance and management o ...201121669071
rna colony blot hybridization method for enumeration of culturable vibrio cholerae and vibrio mimicus bacteria.a species-specific rna colony blot hybridization protocol was developed for enumeration of culturable vibrio cholerae and vibrio mimicus bacteria in environmental water samples. bacterial colonies on selective or nonselective plates were lysed by sodium dodecyl sulfate, and the lysates were immobilized on nylon membranes. a fluorescently labeled oligonucleotide probe targeting a phylogenetic signature sequence of 16s rrna of v. cholerae and v. mimicus was hybridized to rrna molecules immobilized ...200919561182
validation of a method for the detection of five species, serogroups, biotypes and virulence factors of vibrio by multiplex pcr in fish and seafood.in this work a sequential multiplex pcr system was designed and validated for the detection of most frequent foodborne pathogen vibrio species in fish and seafood (vibrio cholerae, vibrio parahaemolyticus, vibrio vulnificus, vibrio alginoliticus and vibrio mimicus). the method proposed functions in a hierarchical way, being composed of an end-point multiplex pcr to detect the presence of dna belonging to the studied species, followed by multiplex pcr and fragment analysis allowing the viability ...201019913702
mycosis fungoides with verrucous lesions. 199984478
[respiratory problems in patients with muscular dystrophy]. 20113285556
[studies on the blood constituents of the neonates delivered by the induced delivery of the defense medical college method].for the assessment of the influence of the defense medical college planned delivery method upon neonates, the measurement of the blood constituents of the umbilical cord was conducted. statistically speaking, no marked differences were seen between the neonates delivered by the defense medical college method and neonates delivered naturally. the blood constituents investigated were: blood cells, activity of various enzymes, other blood constituents and electrolytes.20123457089
agoraphobia and parental bereavement.the prevalence of parental bereavement was determined in 50 married female outpatients with a dsm-iii diagnosis of agoraphobia and in a control group of married female outpatients diagnosed as having non-psychotic psychiatric disorders other than agoraphobia. the two groups were matched for age and overall severity of psychiatric symptoms. compared with the general population, the patient control group reported a statistically significant excess of parental, but not maternal, bereavement. the ag ...20123435374
interference of bromide in determination of serum chloride. 20103815810
effect of antihypertensive drugs on insulin, glucose, and lipid metabolism.the close relationship between diabetes and hypertension has been recognized for decades. new information indicates that resistance to insulin action on glucose uptake in peripheral tissues is a common underlying mechanism in hypertension and diabetes. in prospective trials, the effects of antihypertensive agents on insulin sensitivity and lipoprotein metabolism have been evaluated. both beta-blockers and thiazide diuretics worsen insulin resistance and deteriorate lipoprotein metabolism. angiot ...20061828417
effect of antihypertensive drugs on insulin, glucose, and lipid metabolism.the close relationship between diabetes and hypertension has been recognized for decades. new information indicates that resistance to insulin action on glucose uptake in peripheral tissues is a common underlying mechanism in hypertension and diabetes. in prospective trials, the effects of antihypertensive agents on insulin sensitivity and lipoprotein metabolism have been evaluated. both beta-blockers and thiazide diuretics worsen insulin resistance and deteriorate lipoprotein metabolism. angiot ...20061828417
[postoperative esophageal stenosis (role of the laryngologist)]. 2002153373
noninvasively diagnosed peripheral arterial disease as a predictor of mortality: results from a prospective study.intermittent claudication has been reported in previous studies to approximately double the risk of subsequent mortality. however, a history of claudication is often present in the absence of significant peripheral arterial disease (pad) and absent in the presence of pad. for this reason we evaluated the association between large-vessel and small-vessel pad, measured by highly reliable and valid noninvasive tests, and mortality in 567 older subjects from a defined population followed-up for an a ...20144028377
physiology of the upper airways in allergic disease. 20143902190
[simultaneous detection of vibrio cholerae o1 and o139 by real-time quantitative pcr].targeting the specific o-antigen gene cluster, a taq-man real-time fluorescence pcr assay was developed to detect 01 and o139 vibrio cholera concurrently and avoid frequently occurred false positives.201020364601
interaction between vibrio mimicus and acanthamoeba castellanii.vibrio mimicus is a gram-negative bacterium, which causes gastroenteritis and is closely related to vibrio cholerae. the environmental reservoir of this bacterium is far from defined. acanthamoeba as well as vibrio species are found in diverse aquatic environments. the present study was aimed to investigate the ability of a. castellanii to host v. mimicus, the role of bacterial protease on interaction with a. castellanii and to disclose the ability of cysts to protect intracellular v. mimicus. c ...201020454692
[intramural pseudo-diverticulosis of the oesophagus]. 199898947
comparison of specificity of agonist and antagonist radioligand binding to beta adrenergic receptors. 199819707
development of a single base extension-tag microarray for the detection of pathogenic vibrio species in seafood.in this study, a single base extension-tag array on glass slides (sbe-tags) microarray was established to detect the seven leading seafood-borne pathogens, including vibrio parahaemolyticus, vibrio cholerae, vibrio vulnificus, vibrio mimicus, vibrio alginolyticus, vibrio anguillarum, and vibrio harveyi. three multiplex pcr assays were developed to specifically target the following species with individual gene markers, which are aads, tdh, and trh for v. parahaemolyticus; col, toxr, and vvh for v ...201021161212
dietary medicinal plant extracts improve growth, immune activity and survival of tilapia oreochromis mossambicus.the effects of supplementing diets with acetone extract (1% w/w) from four medicinal plants (bermuda grass cynodon dactylon, h(1), beal aegle marmelos, h(2), winter cherry withania somnifera, h(3) and ginger zingiber officinale, h(4)) on growth, the non-specific immune response and ability to resist pathogen infection in tilapia oreochromis mossambicus were assessed. in addition, the antimicrobial properties of the extract were assessed against vibrio alginolyticus, vibrioparahaemolyticus, vibri ...200920735646
torsade de pointes: a maximal vulnerability arrhythmia. 20143966319
[economic aspects of the sao-method in routine orthopedic surgery]. 20154650689
[newly designed ultraviolet light monochromator]. 20154542243
[retinal neovascularization: topological, morphological and hemodynamic features (author's transl)]. 199991317
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