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reference ranges and ideal patient values for blood cholesterol. can there be reconciliation?reference ranges for blood cholesterol levels reflect the nationwide epidemic of "silent" hypercholesterolemia. as such, reference ranges for cholesterol levels without accompanying designation of levels associated with increased coronary risk are misleading and falsely comforting to physicians and patients. in a recent continent-wide study of 108 academically based clinical laboratories, highly variable reference ranges were observed, the ranges having been derived from a vast assortment of sou ...20133754717
change of blood pressure over five years in childhood and adolescence.we compared blood pressure (bp) values, measured at a five year interval, in 2,777 boys and girls aged 4-18 years. bp values were converted into height and sex adjusted standard deviation unit scores. correlation coefficients were significant but low for systolic bp score: 0.19 in boys and 0.15 in girls, and were even lower for diastolic bp score. these correlations are weaker than those observed for overall bp values (boys: 0.33, girls: 0.23), weight (0.82, 0.75) and height (0.87, 0.80). the pr ...20133757275
regional myocardial protection by retrograde coronary sinus infusion of cardioplegic solution.antegrade administration of a cardioplegic solution in the presence of a coronary artery stenosis may lead to the heterogeneous distribution of the agent and poor myocardial cooling distal to a vessel stenosis. to determine the effects of retrograde coronary sinus infusion of cardioplegic solution, coronary stenosis was created in the canine preparation by occluding the left circumflex artery (lcx) during cardioplegic arrest. left ventricular global and regional function (assessed by sonomicrome ...19863769184
[pathogenesis and treatment of arterial hypertension in hypothyroidism patients]. 20133784472
haemothorax in the course of chickenpox. 20133787528
antibiotic resistance patterns of vibrio mimicus isolated from human and environmental sources in bangladesh.twenty-five environmental and 19 clinical strains of vibrio mimicus were tested for antibiotic susceptibility patterns. environmental strains were resistant to streptomycin, kanamycin, and trimethoprim-sulfamethoxazole; clinical strains were susceptible. environmental strains showed variable resistance to ampicillin (44%), but clinical strains were susceptible. all strains tested were susceptible to chloramphenicol and gentamicin.19863789697
local anaesthetics. iv--synthesis and activity of 2-(n-substituted or n,n-disubstituted aminoacetamido)-4- or -4,5-substituted thiazoles.synthesis is described of eleven new 2-(n-substituted or n,n-disubstituted aminoacetamido)-4- or -4,5-substituted thiazoles (i). their hydrochlorides were screened for local anaesthetic activity on frogs by the sciatic plexus method and the activity compared with that of procaine.20133792539
the role of the paediatric endoscopist.the role of the paediatric endoscopist has been a changing one. when i first commenced at the royal alexandra hospital for children in sydney 25 years ago, paediatric endoscopy of the upper respiratory tract and oesophagus was a limited and poorly defined field. most endoscopic examinations were requested, often reluctantly, by a paediatrician in the investigation of stridor. in fact my first consultation involved instructions to perform a laryngoscopy, but not a bronchoscopy and general anaesth ...20133805880
meta-analyses of randomized controlled trials.a new type of research, termed meta-analysis, attempts to analyze and combine the results of previous reports. we found 86 meta-analyses of reports of randomized controlled trials in the english-language literature. we evaluated the quality of these meta-analyses, using a scoring method that considered 23 items in six major areas--study design, combinability, control of bias, statistical analysis, sensitivity analysis, and application of results. only 24 meta-analyses (28 percent) addressed all ...20133807986
interference of bromide in determination of serum chloride. 20103815810
cervical lymph node metastases with occult primary.patients with cervical metastases from an unknown primary tumour present both diagnostic and therapeutic problems. twenty patients with a metastatic lesion in the neck, in whom the primary tumour was not found despite extensive, diagnostic procedures, were treated in the department of otolaryngology, linköping university hospital, during a 14-year period (1971-1984). since 1975 tonsillectomy has been performed regularly on the affected side as a part of the diagnostic procedure. eight patients w ...20123815873
[pacemaker implantation in infants and children for atrioventricular block]. 20143819491
isolation and characterization of neuropeptide y from porcine intestine.the isolation and primary structure of intestinal neuropeptide y (npy) is described. the peptide was purified from porcine intestinal extracts using a chemical assay and radioimmunoassay for npy. the amino acid sequence of this peptide is: tyr-pro-ser-lys-pro-asp-asn-pro-gly-glu-asp-ala-pro-ala-glu-asp-leu-ala- arg-tyr-tyr- ser-ala-leu-arg-his-tyr-ile-asn-leu-ile-thr-arg-gln-arg-tyr-nh2. this the structure of intestinal npy is identical to the npy of brain origin.19853838090
physiology of the upper airways in allergic disease. 20143902190
1h-15n heteronuclear nmr studies of escherichia coli thioredoxin in samples isotopically labeled by residue type.ten samples of escherichia coli thioredoxin were individually isotopically enriched by residue type via growth of an appropriate auxotrophic strain on media supplemented with one 2h, 15n-enriched amino acid. 1h observe-heteronuclear decoupling experiments were conducted on these samples making use of the 95-hz 1h-15n amide j1 coupling. subtraction of near-resonance from off-resonance 15n decoupled spectra generated difference patterns corresponding only to protons directly bonded to 15n nuclei. ...19853910099
[conservative treatment of injuries of the liver]. 20143911104
viruses and immune reactions in the liver.the immunopathology of hepatitis b and delta virus infections of the liver are reviewed. it is clear there are several antigen/antibody systems of importance in acute and chronic hbv infection. antibodies to hbs, hb core/hbe and antibodies to dane specific determinants are involved in virus neutralisation. elimination of virus infected hepatocytes is dependent on recognition of viral determinants in association with hla proteins on the infected hepatocytes by cytotoxic t cells. the hla protein d ...19853911368
family history of cancer among cancer patients.family history of cancer was examined for 9,131 cancer patients who were reported to the aichi cancer registry in 1979-1981, and were over 20 years old at diagnosis. the rate of patients whose parents and/or siblings had cancer of any site was 24.5%. the rate was 9.2% for father, 8.4% for mother, 6.0% for brother(s), and 5.2% for sister(s). a significant site concordance between study patient and family member with cancer was observed for cancer of the breast, colon and rectum, and stomach. the ...20143920100
overdose with baclofen. 20143945841
growth hormone modulates serum cholinesterase.adult male rats have lower serum cholinesterase activity levels than adult female rats and hypophysectomized male rats have higher activity levels than sham-operated males (similar to control females). gh administered to hypophysectomized male rats abolishes the effect of hypophysectomy on serum cholinesterase. adult male rats treated neonatally with monosodium l-glutamate to induce arcuate nucleus lesions of the hypothalamus have higher serum cholinesterase activities and decreased serum gh con ...19863948777
velopharyngeal insufficiency in children: the variable clinical presentation. 20143957645
torsade de pointes: a maximal vulnerability arrhythmia. 20143966319
blood transfusion and athletics. games people play. 20143974666
[persistent chlamydia infection in cell culture]. 19853993192
noninvasively diagnosed peripheral arterial disease as a predictor of mortality: results from a prospective study.intermittent claudication has been reported in previous studies to approximately double the risk of subsequent mortality. however, a history of claudication is often present in the absence of significant peripheral arterial disease (pad) and absent in the presence of pad. for this reason we evaluated the association between large-vessel and small-vessel pad, measured by highly reliable and valid noninvasive tests, and mortality in 567 older subjects from a defined population followed-up for an a ...20144028377
[in vitro fertilization in reduced male fertility]. 20134029742
repeatability of phasic muscle activity: performance of surface and intramuscular wire electrodes in gait analysis.repeatability is an important consideration for gait analysis data that are being used as an adjunct to clinical decision making. an index of repeatability may be based on a statistical criterion (variance ratio) that reflects similarity of wave forms over a number of identical cycles. the purpose of this study was to use the variance ratio to assess the repeatability of phasic muscle activity recorded with surface and bipolar intramuscular wire electrodes during gait on 10 normal subjects. vari ...20134032106
local cerebral glucose utilization in normal female rats: variations during the estrous cycle and comparison with males.the quantitative 2-[14c]deoxyglucose autoradiographic method was used to study the fluctuations of energy metabolism in discrete brain regions of female rats during the estrous cycle. a consistent though statistically nonsignificant cyclic variation in average glucose utilization of the brain as a whole was observed. highest levels of glucose utilization occurred during proestrus and metestrus, whereas lower rates were found during estrus and diestrus. statistically significant fluctuations were ...19854040921
the distribution of lactate dehydrogenase isoenzymes in adult human achilles tendons.the authors have studied the ldh isoenzymes distribution in the human achilles tendons. connection between the age, sex, and enzyme activity were not found. in the achilles tendon were found strong activity of isoenzymes iv and v (m-type), and controversy them moderate activity of isoenzymes i and ii (h-type).19854065385
[organization of the regular schedules of forensic-medical experts at a bureau of forensic medical examinations]. 20144071589
[factors determining the severity of traumatic lesions of the popliteal artery]. 20134073583
distribution and elimination of [14c]-hexachlorobenzene after single oral exposure in cod (gadus morhua) and flounder (platichthys flesus).distribution and elimination of hexachlorobenzene (hcb) after administration to cod (gadus morhua) of a single oral dose of 5 microci [14c]hcb/100 g body weight were studied by whole-body autoradiography and liquid scintillation counting. to obtain some information on the physicochemical properties of the radiolabeled compounds, whole-body autoradiography was performed exposing parallel sagittal sections, treated at -20 degrees c, evaporated at 50 degrees c, and extracted separately with polar a ...20144078931
[prostatectomy and amputatio recti]. 20144103378
letter: brain-levels of monoamine oxidase in depression. 20144136104
letter: lipolysis induced by coffee and tobacco: its modification by insulin. 19734148444
the purification of portuguese man-of-war nematocyst toxins by gel diffusion. 19734150989
the feasibility of an assistant to the physician. 20144152241
the vascular pathology of thrombotic thrombocytopenic purpura. an immunohistochemical and ultrastructural study. 20144161747
the vaccination of man and other animals against rabies. 19734207636
galvanic skin responses (gsr) and plethysmographic changes induced by chlorimipramine, imipramine and placebo. 20144209521
the response of the pituitary-adrenal system to a stressful stimulus: the effect of conditioning and pentobarbitone treatment. 19654284301
[new studies on a relationship between hemoglobins and rhesus factors in africans]. 20144308643
[new studies on a relationship between hemoglobins and rhesus factors in africans]. 20144308643
the use of multivariate techniques in the study of skeletal populations. 20144313256
behavioral studies of noise-induced hearing loss in primates: loudness recruitment. 20154351038
affinity chromatography of cytosolic nad-linked glycerol-3-phosphate dehydrogenase from normal and neoplastic mammalian tissues. 19744368387
virus infections of horses at newmarket, 1972 and 1973. 19744375339
[the effect of treatment by cocaine on the fixation and liberation of noradrenaline in different peripheral organs of the rat]. 19744411143
immobilon in the otter. 20154450432
[effects of cooking on the content of some vitamins in various species of frozen fish]. 20154480150
observations on pulmonary function testing and blood-gas analysis in children. 20154503927
[are reforms of dental education necessary?]. 20154510183
[newly designed ultraviolet light monochromator]. 20154542243
software printout. 20154542580
seventy-five years of radiological physics. 20154585010
[lysis of the femoral neck and coxa vara (author's transl)]. 20154588540
[serial hemocultures with warren's medium in patients with positive machado-guerreiro reaction]. 20154624192
[economic aspects of the sao-method in routine orthopedic surgery]. 20154650689
[note on psychopathology of delusions]. 20154671572
[survival of human amniotic cells cooled to very low temperature with various cryoprotective agents and various cooling rates]. 19724681354
influence of kynuremate on cholesterol and fatty acid synthesis in isolated perfused rat liver. 19734684700
[double electron-nuclear resonance of free radicals in gamma-irradiated glycine single crystals]. 20164701432
in vitro studies on the control of trophoblast outgrowth in the mouse. 19734729947
self-evaluation by first-year medical students in a clinical science programme. 20164771337
[coefficient of heat exchange of the human body by convection, as a function of position, of the subject's activity and of the environment]. 19734807371
hypersomatotropism and acanthosis nigricans in two brothers. 20164843205
behaviour of carbonic anhydrase in the uterus of hens during moult. 20164958241
[sensitization by metabolic inhibitors of isolated intestine of rats to the effects of sympathomimetics. effects of fluoro-acetate and sodium mitride]. 19704987116
enterotoxin production by vibrio cholerae and vibrio mimicus grown in continuous culture with microbial cell recycle.we have examined the effect of complete cell recycle on the production of cholera toxin (ct) by vibrio cholerae and ct-like toxin by vibrio mimicus in continuous culture fermentations. complete cell recycle was obtained by filtering culture fluids through amicon hollow fibers with an exclusion limit of 100,000 daltons (h1p100-20) and returning the concentrated cell slurry to the fermentor. a single 1-liter laboratory fermentor system modified with this recycle loop was capable of producing over ...19836357081
purification of enterotoxins from vibrio mimicus that appear to be identical to cholera toxin.cholera toxin-like (ct-like) enterotoxins produced by two strains of vibrio mimicus, 61892 and 63616, isolated from diarrhea patients in bangladesh, were purified, and their physicochemical, biological, and immunological properties were compared with those of ct produced by classical vibrio cholerae 569b. the ct-like toxins were produced by lincomycin-resistant mutants grown in the presence of lincomycin at 200 micrograms/ml for strain 63616 and 250 micrograms/ml for strain 61892 and were purifi ...19846469354
experimental studies on the pathogenicity of vibrio mimicus strains isolated in bangladesh.vibrio mimicus, a newly described species of the genus vibrio has been isolated from stools of 14 patients with diarrhoea. live cells of all the 14 strains tested caused accumulation of fluid in rabbit gut loops and diarrhoea in infant rabbits. culture filtrates of all the strains caused increased capillary permeability in rabbit skin; however, five of the filtrates resembled cholera toxin in that they gave positive reactions in rabbit loops, chinese hamster ovarian and mouse adrenal cell monola ...19846517767
production of cholera toxin-like toxin by vibrio mimicus and non-o1 vibrio cholerae: batch culture conditions for optimum yields and isolation of hypertoxigenic lincomycin-resistant mutants.vibrio mimicus 61892, isolated in 1977 from a case of watery diarrhea in bangladesh, produces an enterotoxin which possesses activity in y-1 mouse adrenal cells and in rabbit ileal loops which is identical to the prototype cholera toxin (ct) produced by vibrio cholerae 569b. the neutralization of the adrenal cell activity of 61892 toxin and 569b ct by homologous and heterologous antisera generates parallel titration curves which show complete neutralization in all cases. paired titrations in the ...19836642640
the newly described pathogenic species vibrio mimicus isolated from human diarrhoeal stools and from a sea water sample.four vibrio strains have been isolated from three patients presenting diarrhoea as well as from a sea water sample; identification proved their belonging to vibrio mimicus, a recently described new pathogenic species. the morpho-cultural and biochemical characteristics of the isolated strains are presented. the distribution and incidence of this newly described species in romania are under study.19836675346
ecology of non-o 1 vibrio cholerae in toyama prefecture.the ecology of non-o 1 vibrio cholerae and vibrio mimicus as causes of cholera-like diarrhea or seafood-associated gastroenteritis has been investigated in toyama prefecture since 1980. the relationship between biological or serological characteristics of the isolates and their enteropathogenicity is discussed. overall isolation rates from river water, sea water, and fish were 24.0, 59.5, and 33.7%, respectively, the isolation frequency being, in general, extremely high in the summer season, alt ...19846738382
medium-dependent production of extracellular enterotoxins by non-o-1 vibrio cholerae, vibrio mimicus, and vibrio fluvialis.fluid accumulation at 4 h in the intestines of suckling mice enabled us to distinguish non-o-1 vibrio cholerae, v. mimicus, and v. fluvialis clinical isolates from environmental isolates. enterotoxin production was culture medium dependent. filtrates of cultures grown in tryptic soy broth without glucose but with added 0.5% nacl did not exhibit marked enterotoxin activity in the assay. culture filtrates of all clinical strains grown in brain heart infusion broth supplemented with 0.5% nacl induc ...19836824317
numerical taxonomy of vibrio cholerae and related species isolated from areas that are endemic and nonendemic for cholera.a total of 165 strains of vibrios isolated from clinical and environmental sources in the united states, india, and bangladesh, 11 reference cultures, and 4 duplicated cultures were compared in a numerical taxonomic study using 83 unit characters. similarity between strains was computed by using the simple matching coefficient and the jaccard coefficient. strains were clustered by unweighted average linkage and single linkage algorithms. all methods gave similar cluster compositions. the estimat ...19836874901
disease from infection with vibrio mimicus, a newly recognized vibrio species.clinical and epidemiologic characteristics of disease associated with a newly described nonhalophilic vibrio species, vibrio mimicus, were identified by studying isolates from 21 patients referred to the centers for disease control between 1977 and 1981. two isolates were from the ears of patients with otitis who had recently been exposed to seawater. nineteen isolates were from stool samples; these patients generally had diarrhea, nausea, vomiting, and abdominal cramps, with fever, headache, an ...19836881769
disease from infection with vibrio mimicus, a newly recognized vibrio species. clinical characteristics and edipemiology.clinical and epidemiologic characteristics of disease associated with a newly described nonhalophilic vibrio species, vibrio mimicus, were identified by studying isolates from 21 patients referred to the centers for disease control between 1977 and 1981. two isolates were from the ears of patients with otitis who had recently been exposed to seawater. nineteen isolates were from stool samples; these patients generally had diarrhea, nausea, vomiting, and abdominal cramps, with fever, headache, an ...19836881770
colonization of the rabbit small intestine by clinical and environmental isolates of non-o1 vibrio cholerae and vibrio mimicus.we examined the capability of 12 isolates of non-cholera toxin-producing o1 and non-o1 vibrio cholerae to colonize the small intestine of adult rabbits and cause diarrhea. using the removable intestinal tie-adult rabbit diarrhea model, we found that eight environmental isolates that showed no or marginal biological activity in other diarrhea models (rabbit ileal loop, infant rabbit, and suckling mouse) appeared to be incapable of attaching to and colonizing, even transiently, the small intestina ...19836885157
characterization of biochemically atypical vibrio cholerae strains and designation of a new pathogenic species, vibrio mimicus.biochemically atypical strains classified as vibrio cholerae were characterized by biochemical reactions, serology, antibiotic susceptibility testing, and deoxyribonucleic acid relatedness. strains with the following atypical reactions were shown to be v. cholerae: mannose negative, mannitol negative, lysine decarboxylase negative, no growth in the presence of 5% nacl, salicin and cellobiose positive. sucrose-negative strains were shown to constitute a new species, vibrio mimicus, whose type str ...19817037833
iron transport genes of the pjm1-mediated iron uptake system of vibrio anguillarum are included in a transposonlike structure.the pjm1 genes encoding the proteins involved in iron transport in the anguibactin iron uptake system were found to be flanked by insertion sequences in a composite transposonlike structure. these vibrio anguillarum insertion sequences, isv-a1 and isv-a2, are related to is903, is102, and the isvs found in vibrio parahaemoliticus, vibrio mimicus, and non-o1 vibrio cholerae flanking various tdh (thermostable direct hemolysin) genes. the inverted repeats at the ends of isv-a1 and isv-a2 have no mor ...19957568465
[in vitro antibacterial activity of balofloxacin (blfx) against isolates from patients with bacterial enteritis].in vitro antibacterial activity of balofloxacin (blfx), a newly developed fluoroquinoline, was compared with that of norfloxacin (nflx), ofloxacin (oflx) and ciplofloxacin (cpfx). bacterial strains used in this experiment were freshly isolated from patients with infectious enteritis just before blfx therapy. the isolates were 43 strains of vibrio cholerae o1, 1 strain of campylobacter sp., 4 strains of aeromonas spp., 3 strains of plesiomonas shigelloides, 1 strain of vibrio mimicus and 1 strain ...19957594800
vibrio mimicus arylesterase has thioesterase and chymotrypsin-like activity.a vibrio mimicus serine arylesterase and an escherichia coli thioesterase/serine protease share 49.4% amino acid identity. the arylesterase has thioesterase activity for benzoyl-coa and chymotrypsin-like activity for n-carbobenzoxy-l-phenylalanine p-nitrophenyl ester (nbpnpe). the gene encoding the v. mimicus enzyme is designated etpa. substituting ser31 of the v. mimicus enzyme with a glycine or an alanine altered its activity. in comparison with wild type enzyme, the s31a enzyme showed a 5-fol ...19957646502
the sixth and seventh cholera pandemics are due to independent clones separately derived from environmental, nontoxigenic, non-o1 vibrio cholerae.the dna sequences of the asd genes from 45 isolates of vibrio cholerae (19 clinical o1 isolates, 2 environmental nontoxigenic o1 isolates, and 24 isolates with different non-o1 antigens) were determined. no differences were found within either sixth- or seventh-pandemic isolates; however, variation was found between the two forms and among the non-o1 isolates. o139 isolates had sequences identical to those of seventh-pandemic isolates. phylogenetic trees with vibrio mimicus as the outgroup sugge ...19957768818
fatal gastroenteritis due to vibrio fluvialis and nonfatal bacteremia due to vibrio mimicus: unusual vibrio infections in two patients. 19947811875
existence of a novel hemagglutinin having no protease activity in vibrio mimicus.the protease elaborated by vibrio mimicus is known to possess hemagglutinating ability to chicken erythrocytes, the well-known ha/protease. a non-protease hemagglutinin (ha) with strong agglutinating ability towards rabbit erythrocytes was obtained from 32 hr culture supernatant of a pathogenic environmental strain of v. mimicus. this ha (v. mimicus ha: vmha) appeared stable at relatively higher temperature and agglutinated the erythrocytes from rabbit, guinea pig and mouse but not the erythrocy ...19947968677
identification of the siderophores from vibrio hollisae and vibrio mimicus as aerobactin.hydroxamate siderophores were purified from low-iron cultures of vibrio hollisae atcc 33564 and vibrio mimicus atcc 33653. by a combination of 1h and 13c nmr spectroscopy, fast atom bombardment mass spectrometry, and compositional analysis, both of the siderophores were identified as aerobactin, a citrate-based dihydroxamate siderophore, which is highly prevalent in species of the family enterobacteriaceae. four other clinical strains belonging to these species also produced aerobactin. in respo ...19948013878
a gene for the enterotoxin zonula occludens toxin is present in vibrio mimicus and vibrio cholerae o139.the presence of the zonula occludens toxin (zot) gene, which encodes an enterotoxin produced by serotype o1 strains of the pathogenic bacterium, vibrio cholerae, in addition to cholera toxin, was investigated in selected strains of v. mimicus and the new pandemic v. cholerae non-o1 serotype o139. the zot gene was detected by polymerase chain reaction (pcr) amplification, using sets of primers based on the sequence of the v. cholerae o1 zot sequence. pcr amplification of genomic dnas of both chol ...19948050719
an analysis of the v1 and v2 regions of vibrio cholerae and vibrio mimicus 16s rrna.the v1 and v2 variable regions of the 16s rrna gene of three strains of v. cholerae and one strain of v. mimicus were amplified by pcr. fragments containing both regions were cloned into m13mp18 using smal and sequenced by the dideoxy method. the 263-bp sequence from a strain isolated during the 1991 cholera outbreak in brazil was deposited in genbank under the accession number l05178. except for an extra g in one of the strains, the three v. cholerae sequences were identical. the v. mimicus seq ...19948090995
vibrio mimicus with multiple toxin types isolated from human and environmental sources.a collection of 13 strains of vibrio mimicus, including both clinical and environmental isolates from different geographic regions, was examined for various toxins. one strain of environmental origin produced cholera-like toxin (ct) which was completely absorbed with anti-ct immunoglobulin g, five strains produced a haemolysin that cross-reacted with the thermostable direct haemolysin of v. parahaemolyticus and dna from two strains hybridised with a dna probe specific for the heat-stable enterot ...19948114069
evaluation of virulence factors in environmental isolates of vibrio species.strains of vibrio parahaemolyticus, vibrio fluvialis and vibrio mimicus isolated from seafood and seawater were examined for characteristics related to infectivity, such as enzymatic activity and animal assays. all strains hydrolysed dna, starch, gelatin and chitin. variable results were obtained with the haemolysin, chondroitin, collagen, elastin and lecithin tests. production of thermostable direct haemolysin by v. parahaemolyticus was detected in 7.1% strains derived from seafood and 2% from ...19938139466
nucleotide sequence of a novel arylesterase gene from vibro mimicus and characterization of the enzyme expressed in escherichia coli.a gene coding for an arylesterase of vibrio mimicus was cloned. sequence determination reveals that the esterase gene has an open reading frame of 600 nucleotides which encodes a protein of m(r) 22,300. the deduced amino acid sequence contain a pentapeptide gdsls (residues 27-31), which was also found in the phospholipid-cholesterol acyltransferase from aeromonas hydrophila. substitution of ser-29 by alanine or cysteine in the cloned gene abolished the esterase activity in the tributyrin plate a ...19948141782
vibrio mimicus. 19938294347
acute diarrhoea caused by vibrio mimicus in calcutta.out of 539 acute diarrhoea cases studied, vibrio mimicus was isolated as a sole pathogen in the faeces of 7 (1.3%) cases. the chief clinical presentations of the seven cases were watery diarrhoea and vomiting. bloody diarrhoea was observed in 2 (28.5%), abdominal pain in 2 (28.57%) and fever in one (14.29) cases. all cases could be effectively treated with ors except 3 (42.85%) cases who required iv ringer's lactate. all v. mimicus strains isolated in the study were uniformly susceptible to tetr ...19938294349
structural studies of the vibrio mimicus w-26768 o-antigen polysaccharide.the structure of the vibrio mimicus w-26768 o-antigen polysaccharide has been investigated by sugar and methylation analyses, smith degradation, and nmr spectroscopy. it is proposed that it is composed of chains of beta-(1-->4)-linked 3,6-dideoxy-3-[(r)-3-hydroxybutyramido]-d-glucopyranosyl residues (d-qui(p)3nr), approximately 60% of which are substituted in the 2-position with 2-acetamido-2-deoxy-alpha-d-galactopyranosyl groups. the polysaccharide does not seem to be composed of oligosaccharid ...19938324759
high prevalence of thermostable direct hemolysin (tdh)-like toxin in vibrio mimicus strains isolated from diarrhoeal patients.a total of 17 isolates of vibrio mimicus from patients, 29 from environment and 2 from food was examined for toxigenicity. sixteen (94%) clinical isolates and one (50%) from food produced tdh-like toxin, whereas none of the environmental isolates did so. the food from which v. mimicus with tdh-like toxin production was isolated, was one which had caused food poisoning. only one environmental strain produced ct-like toxin, whilst st-like toxin was not detected from any strains tested.19938348932
studies on hemolytic action of a hemolysin produced by vibrio mimicus.some properties and mechanism of action of a hemolysin (vmh) produced by an enteropathogenic vibrio mimicus strain was examined. vmh was heat-labile and inhibited by addition of divalent cations, including ca2+, mg2+ and mn2+. the hemolysis by vmh was inhibited by incubating with gangliosides, suggesting that the ganglioside was the binding site on the erythrocyte membrane for vmh. existence of a galactose moiety on reducing end of the ganglioside molecule and a sialic acid on the galactose moie ...19938355623
phylogenetic relationships of marine bacteria, mainly members of the family vibrionaceae, determined on the basis of 16s rrna sequences.the phylogenetic relationships of 50 reference strains, mostly marine bacteria which require na+ for growth, were determined on the basis of 600 16s rrna nucleotides by using reverse transcriptase sequencing. strains belonging to 10 genera were included (four genera of the family vibrionaceae, the genus aeromonas of the family aeromonadaceae, and the genera alteromonas, marinomonas, shewanella, pseudomonas, and deleya). the sequences were aligned, the similarity values and evolutionary distance ...19938427811
development of an enzyme-labeled oligonucleotide probe for the cholera toxin gene.an alkaline phosphatase-conjugated 30-mer oligonucleotide probe was developed to detect the cholera toxin gene (ctx) in vibrio cholerae o1. for rapid identification, v. cholerae o1 was grown on selective agar (thiosulfate-citrate-bile salts agar) or in alkaline peptone water and organisms were transferred directly to nylon membranes. lysis of cells, denaturation of dna, neutralization, and hybridization were carried out on the membrane. these procedures required only 3 h for completion. the resu ...19938501233
crystallization and preliminary x-ray crystallographic analysis of arylesterase from vibrio mimicus.single crystals of arylesterase (ec 3.1.1.2) from vibrio mimicus have been obtained from ammonium sulfate as a precipitant at room temperature for 2 months. the present crystals diffract up to 2.2 a resolution and belong to monoclinic space group p2(1). the cell dimensions are a = 55.65(1) a, b = 53.46(1) a, c = 65.79(1) a, and beta = 106.54(1) degrees. there are two molecules of molecular weight 22 kda in an asymmetric unit with a solvent content of 43%.19958528091
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