| microbiological biosynthesis of biotin. | | 1979 | 108512 |
| immobilization of pyridoxal 5'-phosphate and pyridoxal 5'-phosphate-dependent enzymes on sepharose. | | 1979 | 108513 |
| discontinuous duplication of both strands of virus 2c dna. | | 1979 | 108519 |
| response of periapical pathosis to endodontic treatment in monkeys. | response of induced periapical lesions in monkeys to a conventional endodontic technique was investigated at varying periods ranging from 15 to 365 days after treatment. the findings indicate that response to treatment is influenced by the extent of the root canal filling, the time lapse between treatment and death, and the presence or absence of bacteria in the apical portion of the canal. | 1979 | 108648 |
| acquisition and discrimination of appetitively and aversively conditioned heart rate responses in rhesus monkeys. | this report describes the acquisition of a conditional heart rate response to both classical aversive and appetitive conditioning in eight rhesus monkeys (macaca mulatta). the behavioral paradigm consisted of two discrete one minute tones followed by the corresponding unconditional stimuli, i.e., electric shock or purina monkey chow. a third tone followed by neither food nor shock served as a discriminative stimulus (ds). the conditional heart rate responses developed in two phases. the first ph ... | 1978 | 108656 |
| [on the antimicrobial activity of propolis and propolis constituents (author's transl)]. | after a survey of the literature on the antimicrobial activity of the bee product propolis, the authors discuss their own findings as compared to the chemotherapeutical agents streptomycin, oxytetracycline, chloramphenicol, nystatin, griseofulvin and sulphamerazine. according to the results obtained by testing 25 isolated constituents on bacillus subtilis, staphylococcus aureus, candida albicans and trichophyton mentagrophytes, the antimicrobial properties of this mixture of natural substances a ... | 1979 | 108687 |
| probenecid-induced increase of 5-hydroxytryptamine synthesis in rat brain, as measured by formation of 5-hydroxytryptophan. | probenecid blocks the efflux of 5-hydroxyindole acetic acid (5-hiaa) from the central nervous system, and has therefore been used for turnover measurements of central 5-hydroxytryptamine (5-ht). this substance also elevates tryptophan (tp) levels in rat brain. in this investigation, the time courses of probenecid and tp levels in rat serum and brain after administration of probenecid were studied. maximal levels of probenecid were reached within 15 min, followed by 50% decrease of serum tp and a ... | 1979 | 108699 |
| quipazine-induced stimulus control in the rat. | | 1979 | 108704 |
| hypnotic susceptibility to various depressants in rats selected for differential ethanol sensitivity. | ma rats, bred for greater motor impairment following subhypnotic doses of ethanol, were found to be more sensitive to the hypnotic effects of phenobarbital and chloral hydrate than were la rats. in addition, the previously reported finding of a difference between the two lines of rats in duration of loss of righting reflex following a hypnotic dose of ethanol was replicated. the results are discussed in terms of a phenotypic difference in central nervous systems sensitivity to a range of sedativ ... | 1979 | 108710 |
| the effect of clonidine withdrawal on total 3-methoxy-4-hydroxyphenylglycol in the rat brain. | the effect of clonidine withdrawal on the brain norepinephrine system was studied in the rat. clonidine suppresses brain total 3-methoxy-4-hydroxyphenylglycol (mhpg) significantly and no tolerance to this effect was observed up to 21 days. cessation of clonidine treatment resulted in an elevation in the level of brain total mhpg. the suitability of the clonidine withdrawal syndrome as a model of bipolar depression is discussed. | 1979 | 108714 |
| sex differences following pharmacological manipulation of the cholinergic system by dfp and pilocarpine. | | 1979 | 108719 |
| dietary modification of amphetamine stereotyped behaviour: the action of tryptophan, methionine, and lysine. | rats fed diets high in tryptophan, methionine, and tryptophan plus methionine or lysine were subsequently injected with 10 mg/kg d-amphetamine. the amount of amphetamine-induced stereotyped behaviour observed varied as a function of the dietary addition, with methionine showing the greatest difference to controls, and lysine the least. whole brain catecholamine levels and 5-hydroxytryptamine levels also showed amino acid-specific changes. | 1979 | 108724 |
| [emotional reactivity and neurovegetative balance in the rat (author's transl)]. | it has been shown in male rats of two albino strains, wistar and sprague-dawley, that differences in emotional reactivity were related to physiological differences (bernet and denimal, 1978). particularly the resting heart rate was slower in the more emotionally reactive rats. it has been suggested that emotional reactivity is linked with a certain neurovegetative balance. this possibility was investigated in twelve rats of each strain by means of differential blockade of the autonomic nervous s ... | 1979 | 108736 |
| comparison of the discriminative stimulus properties of nefopam and morphine. | separate groups of rats were trained to discriminate nefopam or morphine from saline in a two-lever operant task. nefopam produced saline-appropriate responding in morphine-trained rats. the highest dose of morphine tested in nefopam-trained rats produced responding appropriate for neither training treatment. this intermediate morphine responding was antagonized by a naloxone treatment that had no effect on the nefopam discrimination. these results suggest that the stimulus properties of nefopam ... | 1979 | 108741 |
| the generation and specificity of cytotoxic t cells raised against syngeneic tumor cells bearing akr/gross murine leukemia virus antigens. | efforts were made to generate c57bl/6 cytotoxic effector cells to a syngeneic leukemia (e{male}g2) bearing akr/gross virus antigens. as we were unable to induce significant cytotoxic activity by immunization with up to 10(8) irradiated e{male}g2 cells, even when cells from such primed animals were subsequently restimulated with e{male}g2 cells in vitro, c57bl/6 mice were immunized with an aliogeneic, virus-producing akr leukemic cell line (akr sl3). peritoneal exudate cells and, to a lesser degr ... | 1979 | 109575 |
| selection and characteristics of a vibrio cholerae mutant lacking the a (adp-ribosylating) portion of the cholera enterotoxin. | after mutagenesis with nitrosoguanidine and selection by immuno-halo techniques, an avirulent mutant, designated texas star-sr, which produces no detectable a (active; adp-ribosylating) region of the cholera enterotoxin (choleragen) but produces the b region (choleragenoid) in amounts similar to the hypertoxinogenic wild-type parent vibrio cholerae (biotype e1 tor serotype ogawa), has been isolated. the mutant retains the colonizing ability, motility, prototrophy, and serologic characteristics o ... | 1979 | 109838 |
| spermatic granuloma of vas deferens after vasectomy in rhesus monkeys and men: light and electron microscopic study. | spermatic granuloma of the vas deferens is a common complication of vasectomy which has received scant morphologic study. this study investigated the light and electron microscopic structure of such granulomas detected in rhesus monkeys (macaca mulatta) and man after various modes of vasectomy and postoperative periods. unilateral experimental vasectomy in monkeys was performed by either silk ligation or clasp occlusion; in 4 of 13 ligated animals and 5 of 5 clasp vasectomized animals granulomas ... | 1979 | 109982 |
| subunit composition of bacillus subtilis rna polymerase during transcription. | | 1979 | 110325 |
| colivirus-t3-coded s-adenosylmethionine hydrolase. | bacteriophage t3 induces an enzyme activity which hydrolyzes s-adenosylmethionine. this s-adenosylmethionine hydrolase is interesting, not only because of its unique activity, but also because the protein has to overcome host restriction [f. w. studier and n. r. movva (1976) j. virol. 19, 136-145]. s-adenosylmethionine hydrolase was purified to homogeneity using affinity chromatography on s-adenosylhomocysteine-sepharose. the enzyme occurs in two forms, a and b. form a consists of the viral pept ... | 1979 | 110588 |
| evidence of a high-activity c type of carbonic anhydrase in human ciliary processes. | carbonic anhydrase activity was found in the ciliary process of fresh human donor eyes, originating from an enzyme antigenically similar to the erythrocyte high-activity isoenzyme hca c. it was sensitive to inhibition by acetazolamide and resistant to inhibition by halides like hca c. the enzyme is probably identical with hca c. its tissue concentration was one fifth to one tenth of that in the human kidney. the erythrocyte low-activity isoenzyme hca b was also found in the processes as a contam ... | 1979 | 110722 |
| immunohistochemical localization of exocrine enzymes in normal rat pancreas. | on the basis of morphological and biochemical differences, the exocrine pancreatic tissue has been divided in peri- and teleinsular regions. in the present study the enzymatic profile of these regions has been investigated by the immunofluorescent technique using antibodies against nine pancreatic enzymes (alpha-amylase, lipase, chymotrypsinogen a, trypsinogen, elastase, carboxypeptidase a and b, dnase and rnase a). these antibodies were specific to their antigens without cross reaction. by immu ... | 1979 | 110872 |
| baseline blood chemistry determinations in the squirrel monkey (saimiri sciureus). | sera from 129 squirrel monkeys, saimiri sciureus, were analyzed for 15 chemical constituents. the values obtained were then analyzed for statistical significance causing the following sets of variables: (1) colony-bred versus noncolony-bred, (2) karyotype (3) vendor, (4) sex and (5) dietary iodine supplementation versus nonsupplementation. calcium, inorganic phosphorous, albumin, uric acid, blood urea nitrogen, glucose, alkaline phosphatase and potassium were high in colony-bred animals. cholest ... | 1979 | 110977 |
| human health effects and air pollution control philosophies. | | 1979 | 110986 |
| membrane potential and reconstitution. | | 1979 | 110998 |
| [melanin-producing strains of pseudomonas aeruginosa]. | | 1979 | 111005 |
| [the recurring urinary tract infection. do immunologic factors play a part?]. | | 1979 | 111109 |
| postnatal development of carrier-mediated absorption of disodium cromoglycate from the rat lung. | | 1979 | 111264 |
| hallucinogen-induced rotational behavior in rats. | | 1979 | 111284 |
| feeding parameters with two food textures after chlordiazepoxide administration, alone or in combination with d-amphetamine or fenfluramine. | chlordiazepoxide (5.0, 10.0 mg/kg) reduced the rate of eating and extended the duration of feeding in a 10 min feeding test. it also reduced the latency to feed. both fenfluramine (1.0 mg/kg) and d-amphetamine (0.25 mg/kg) acted to reduce food intake, but by differing mechanisms. fenfluramine reduced eating rate without affecting eating duration, whilst d-amphetamine reduced eating duration without reducing eating rate. the effects of chlordiazepoxide on feeding parameters were generally additiv ... | 1979 | 111292 |
| effect of neonatal hypothyroidism and delayed l-triiodothyronine treatment on behavioural activity and norepinephrine and dopamine biosynthetic systems in discrete regions of rat brain. | | 1979 | 111295 |
| [ecological aspects of south american trypanosomiasis. xiii. domestic enzootic potential in an area of occurrence of panstrongylus megistus, under epidemiological surveillance]. | | 1978 | 111341 |
| lethal effects of 32p decay on transfecting activity of bacillus subtilis phage phi e dna. | | 1979 | 111411 |
| [quantitative changes in the cellular makeup of the spleen in mice infected with the rauscher leukemia virus and brucella abortus]. | changes in the absolute number of splenic cells of mice infected with rauscher leukemia virus (rlv) and a day later--with living brucella (ba) were studied. the increase in the absolute number of splenic cells of mice infected with rlv + ba was initially caused by the lymphoid tissue reaction to brycella and then by the development of leukemia. the number of reticular cells in the spleen appeared to be larger than the control (rlv) within all observation periods; the number of basophilic normocy ... | 1979 | 111416 |
| a radial immunodiffusion method for the measurement of rat fibrinogen and fibrin degradation products. | the present report describes a technique for the production of monospecific rabbit antiserum to rat fibrinogen and its use in a quantitative radial immunodiffusion assay for measurement of rat fibrinogen and fibrin degradation products. it is a suitable technique for measurement of these parameters in small samples which can be repeatedly obtained from animals the size of rats. | 1979 | 111420 |
| the effect of oral pancreatic enzymes on the intestinal flora of protein-deficient vervet monkeys challenged with vibrio cholerae. | jejunal bacterial flora in 11 protein deficient vervet monkeys (cercopithecus aethiops) and four controls was studied. these were the same animals from an investigation previously reported in which it was shown that pancreatic extract modified the course of cholera infection in protein-deficient monkeys. the present study reports in addition that these animals the fluid in the upper jejunum contained significantly increased numbers of bacteria including enterobacteriaceae compared to its prediet ... | 1979 | 111538 |
| chromosomal evolution in primates: tentative phylogeny from microcebus murinus (prosimian) to man. | the karyotypes of more than 60 species of primates are studied and compared, with the use of almost all existing banding techniques. there is a very close analogy of chromosome banding between the simians studied and man. the quantitative or qualitative variations detected all involve the heterochromatin. it is very likely that all the euchromatin (nonvariable r and q bands) is identical in all the species. approximately 70% of the bands are common to the simians and to the lemurs (prosimians). ... | 1979 | 112030 |
| effect of endo-beta-galactosidase on intact human erythrocytes. | endo-beta-galactosidase, a glycosidase that hydrolyzes gal beta 1-4 glcnac linkages in glycoconjugates, has been used to probe the plasma membrane of human erythrocytes. coomassie blue staining of stroma components separated by sodium dodecyl sulfate-acrylamide gel electrophoresis indicates that treatment of red cells with endo-beta-galactosidase converts protein 3, the anion transporter of the erythrocyte, to a more compact staining band. no other components detected by coomassie staining are a ... | 1979 | 112098 |
| inactivation of slow reacting substance of anaphylaxis (srs-a) by lipoxidase. | lipoxidase (e.c.1.13.1.13) inactivates rat srs-a. the inactivation is time, temperature, and concentration-dependent. linoleic acid and eicosatetraynoic acid inhibit the enzymatic deactivation of the mediator. it is concluded that srs-a is a genuine substrate for lipoxidase and contains a cis, cis-1, 4-pentadiene structure. it is suggested that lipoxidase could play a major role in tissue removal of the mediator during hypersensitivity reactions. | 1979 | 112651 |
| adaptive changes caused by intermittent styrene inhalation on xenobiotic biotransformation. | | 1979 | 112720 |
| alteration of the enucleating erythroblast glycocalyx. | plasmalemmal differentiation of the enucleating normoblast of rabbit and rat was studied by means of cytochemical methods and freeze-etching. staining with colloidal iron revealed about identical amounts of iron particles bound to various areas of the normoblast membrane. cationized ferritin and ruthenium red, likewise, failed in the demonstration of significant changes of the enucleating normoblast glycocalyx. despite these findings the topo-optical staining with toluidine blue showed the plasm ... | 1979 | 112825 |
| stimulation of chloride transport by fatty acids in corneal epithelium and relation to changes in membrane fluidity. | the effect of altering cell membrane lipids on ion transport across isolated corneas was studied. corneas mounted in ussing-type chambers showed a rapid increase in short-circuit current following treatment with a variety of unsaturated fatty acids of varying chain length and unsaturation. measurements of membrane fluidity which utilize immunofluorescence labelling of membrane proteins showed corneal epithelial cell membranes to be significantly more fluid following linoleic acid treatment. upt ... | 1979 | 113032 |
| effect of 2-4-dinitrophenol on intercellular communication in mammalian cardiac fibres. | the effect of 2-4-dinitrophenol (dnp) on cell communication, in canine purkinje fibres, was investigated. it was found that dnp (0.5 mm) suppressed the electrical coupling in about 10 min. this effect of dnp was largely due to an increment in intracellular longitudinal resistance. the longitudinal movement of fluorescein (mol. wt. 320) along purkinje strands, followed with the cut-end method, was also suppressed by dnp (0.5 mm). the decoupling action of dnp was related to release of ca from intr ... | 1979 | 113772 |
| the effect of bilateral ventral noradrenaline bundle lesions on lever pressing for food in rats. | food reward has been associated with activation of noradrenergic mechanisms in the brain. using rats trained to press a lever for food reward, we have investigated the effects of 6-hydroxydopamine lesions, which severly depleted hypothalamic noradrenaline, on the willingness of the rats to press the lever for food reward. we found that performance in the food-rewarded task was significantly impaired following such lesions, and that this was especially marked when the task was made more difficult ... | 1979 | 113830 |
| [bactericidal effect of disinfectants on various strains of mycobacterium tuberculosis]. | | 1979 | 113848 |
| isolation of enzymatically derived fragments of porcine igg and an examination of their reactivity against staphylococcal protein a. | papain digestion of porcine igg in the absence of cysteine resulted in a rather poor yield of fragments (less than 5 per cent). in the presence of cysteine, 70 to 80 per cent of the igg was degradated in 4 h. fragments with molecular weight of about 100,000 and 50,000 were separated by gel filtration. the minor fraction (mol. wt. 100,000) most probably consisted of f(c)2 fragments. fab/c fragments with both fc and fab determinants, and also probably some f(ab)2-like fragments. the f(c)2 fragment ... | 1979 | 113980 |
| a craniometric study of the black and white colobus illiger 1811 (primates: ceropithecoidea). | this study examines the craniometry of black and white colobus monkeys using 1072 specimens representing all the recognized subspecies (after rahm, '70) of the genus. seventy-six measurements were taken on each individual, and examined using canonical variates analysis and clustering by ward's error sum method. the assumptions of the analytical techniques are shown to be met, and the results demonstrated to be stable. examination of the populations for statistical difference and taxonomic distin ... | 1979 | 114059 |
| differences of chemical structures of pseudomonas aeruginosa lipopolysaccharide essential for adjuvanticity and antitumor and interferon-inducing activities. | | 1979 | 114416 |
| organochlorine compounds in adipose tissue of greenlanders and southern danes. | abdominal fat tissue samples from the general population of greenland and from southern denmark were analyzed for o,p'-dde and p,p'-dde [1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene], p,p,-ddt [1,1,1-trichloro-2,2-bis(p-chlorophenyl)-ethane], o,p'-ddt and p,p'-ddd [1,1-dichloro-2,2-bis(p-chlorophenyl)ethane], lindane (1,2,3,4,5,6-hexachlorocyclohexane), aldrin (1,2,3,4,10,10-hexachloro-1,4,4a,5,8,8a-hexahydro-endo-exo-1,4:5,8-dimethanonaphthalene), dieldrin (1,2,3,4,10,10-hexachloro-6,7-epoxy-1, ... | 1979 | 114667 |
| [the course of functional psychoses (author's transl)]. | functional psychoses correspond to a universal reduction of mental and intellectual function which, fundamentally reversible and unspecific, appear as sequelae of disturbances of cerebral function. they are the most common psychoses in medical practice and in hospitals. with the syndrome short test and functional psychosis scale b we now have a mutually equilibrated test system for the measurement of the whole range of severity of functional psychoses. with these two psychopathometric methods a ... | 1979 | 114811 |
| effects of pseudomonas toxin a, diphtheria toxin, and cholera toxin on electrical characteristics of turtle bladder. | rapidly developing changes in the short-circuiting current (isc), conductance (g), and potential (pd) of turtle bladders in na-rich or na-free media are seen after the mucosal addition, at 10 nm, of each of three toxins that contain adp-ribosylation activity: pseudomonas aeruginosa toxin a, diphtheria toxin, and cholera toxin. toxin a irreversibility decreased the isc, pd, and g of bladders in na-rich media and the isc and pd of bladders in na-free media. diphtheria or cholera toxin reversibly i ... | 1979 | 115005 |
| histochemical patterns in human breast tumours. | histochemical investigations carried out on 104 breast tumours (10 benign proliferations and 94 carcinomas) showed that breast carcinomas were characterized by various enzymological equipments in relation to tumour differentiation degree, histogenesis, functional stage, site of tumour cells in the carcinomatous islands or cords and to hormonal factors. tumour cells -- stroma interrelationships study pointed out that high glycolysis (high ldh, nadph2-tr, nadh2-tr activities) and an intense lepa-a ... | 1979 | 115209 |
| nitroblue tetrazolium (nbt) reduction by bacteria. some properties of the reaction and its possible use. | all the s. albus, e. coli and p. aeruginosa strains examined reduced nitroblue tetrazolium (nbt) to dark blue formazan. the amount of formazan produced was proportional to the number of bacteria. under the same growth conditions, an equal number of bacteria of various strains produced different amounts of formazan. however, there were statistically verified differences in the nbt-reduction between the three species examined. the nbt-reduction took place in all phases of growth but was most inten ... | 1979 | 115226 |
| pharmacological modification of experimental tardive dyskinesia. | cebus apella monkeys subjected to chronic haloperidol administration develop neurologic disturbances very similar to neuroleptic-induced acute dystonia human beings. after varying lengths of time, certain monkeys develop a prolonged dyskinetic syndrome resembling tardive dyskinesia (td), as seen clinically. two monkeys with signs of td were given single intramuscular injections of various compounds with known effects on the catecholaminergic, cholinergic, serotoninergic and gaba-ergic neurotrans ... | 1979 | 115227 |
| pancreatic transplantation in cases of diabetes complicated by renal insufficiency. | | 1979 | 115240 |
| effects of methamphetamine and scopolamine on variability of response location. | methamphetamine and scopolamine were studied in monkeys responding under a multiple fixed-ratio fixed-interval schedule of reinforcement. a response on any one of six levers could satisfy the schedule requirements. variability of response location was evaluated in terms of switches, where a switch was defined as a response on one lever followed by a response on a different lever. under baseline conditions the fixed-ratio schedule generated a high rate of responding and a low level of variability ... | 1979 | 115956 |
| sensitization of myelinated nociceptive afferents that innervate monkey hand. | | 1979 | 115969 |
| the effect on bone repair of aspirin cones placed in extraction sockets in dogs: a histopathologic study. | four canines of eight 2-year-old dogs were extracted surgically, a mucoperiosteal flap being raised. a standardized pair of the post-extraction cavities were filled with three aspirin cones, the other pair being used as controls. the flaps were sutured with surgical gut. the dogs were killed at intervals of 2, 4, 6 and 8 weeks; bone blocks including the cavities were resected and decalcified for histologic examination; serial sections were stained with hematoxylin and eosisin. microscopic observ ... | 1979 | 115978 |
| brucella abortus in coyotes. i. a serologic and bacteriologic survey in eastern texas. | prevalence of brucella abortus serum antibodies in coyotes from east central texas was determined by the buffered brucella antigen (card test), rivanol, standard agglutination tube, and cold complement fixation tube tests. eighteen percent (9 of 51) of the coyotes were positive serologically. b. abortus biotype 1 was isolated from various tissues from 7 of 43 coyotes by bacteriologic culture. congenital transmission was found. | 1979 | 116012 |
| renal malakoplakia with renal vein thrombosis. | | 1979 | 116513 |
| folacin deficiency and requirement in the squirrel monkey (saimiri sciuresus). | acute folacin deficiency was studied in eight young squirrel monkeys (saimiri sciureus). half of the animals were fed a semipurified deficient diet (no added folic acid) and half were fed a control diet (0.84 mg of added folic acid per kilogram of dry diet). monkeys fed the deficient diet lost weight and suffered from diarrhea and dehydration leading to the death of one of the animals after 6 weeks. folacin deficiency also was studied in six older animals fed diets containing varying levels of a ... | 1979 | 116540 |
| [investigation on glycogen in the salivary glands of man and many kinds of mammals (author's transl)]. | | 1978 | 116565 |
| study of the anti-tumor effect of polypeptide pineal extract. | bovine pineal polypeptide extract (ppe) exerted an anti-tumor effect on mouse-transplantable tumors: mammary cancer (rsm), squamous cell cervical carcinoma (scc), hepatoma-22a and lympholeukemia lio-1, and had no effect on harding-passey melanoma and leukemia l-1210. it was shown that ppe possessed the ability to decrease the incidence of dmba-induced mammary adenocarcinomas in rats. the daily administration of 0.5 mg ppe prolonged the life span of rats by 25% and failed to influence spontaneous ... | 1979 | 117414 |
| in vitro response to rosette inhibition factor (rif) and rosette restoring factor (rrf) of human lymphocyte after fractionation on a discontinuous density albumin gradient. | in vitro studies on peripheral blood lymphocytes from tuberculous patients and healthy subjects are reported. factors isolated from human serum used in the experiments included the rosette inhibition factor (rif) from serum of tuberculous patients and the rosette restoring factor (rrf) from serum of healthy subjects. lymphocytes were fractionated on a discontinuous bsa gradient. lymphocytes from healthy subjects, sensitive to rif, as well as lymphocytes from tuberculous patients, sensitive to rr ... | 1979 | 117785 |
| cost-benefit studies of vaccinations in finland. | the benefits of vaccination are, in general, recognized. for some diseases these benefits are obvious. when starting or evaluating new vaccination programmes, a more critical approach is needed. before starting vaccination against measles and rubella in finland a cost-benefit study was performed. according to those studies the net benefits of each vaccination would be ca. 100 million finnish marks in 25-30 years. those benefits not measurable in money value of human life, decrease of suffering, ... | 1979 | 118069 |
| construction and characterization of e. coli promoter-probe plasmid vectors. i. cloning of promoter-containing dna fragments. | derivatives of the escherichia coli drug-resistance plasmid pbr316 have been constructed which act as molecular probes for promoter-containing dna restriction fragments from various prokaryotic genomes. the plasmids, designated pbrh1 and pbrh3b, contain a unique ecori restriction site located within the promoter for the tetracycline resistance (tcr) gene. this site was created by the insertion of a chemically synthesized octanucleotide, containing the ecori cleavage sequence, into the hindiii si ... | 1979 | 118083 |
| purification and characterization of a hemolysin produced by vibrio cholerae biotype el tor: another toxic substance produced by cholera vibrios. | a thermolabile direct hemolysin from an el tor cholera vibrio strain has been isolated and partially characterized as a simple protein of ca. 20,000 molecular weight. in addition to its hemolytic activity, the hemolysin is cytotoxic, cardiotoxic, and rapidly lethal. in these respects it resembles the thermostable direct hemolysin/cytotoxin/cardiotoxin/lethal toxin of vibrio parahaemolyticus and certain other bacterial hemolysins, although there are other significant differences. because identica ... | 1979 | 118926 |
| fine structure of mastocytoma cells and mast cells of mice and rats. | | 1979 | 119937 |
| the progressive nature of neoplastic transformation of syrian hamster embryo cells in culture. | | 1979 | 119980 |
| effect of inosine on warm renal ischemia in monkeys and dogs. | | 1979 | 120023 |
| distribution of membrane-bound monoamine oxidase in bacteria. | the distribution of membrane-bound monoamine oxidase in 30 strains of various bacteria was studied. monoamine oxidase was determined by using an ammonia-selective electrode; analyses were sensitive and easy to perform. the enzyme was found in some strains of the family enterobacteriaceae, such as klebsiella, enterobacter, escherichia, salmonella, serratia, and proteus. among strains of other families of bacteria tested, only pseudomonas aeruginosa ifo 3901, micrococcus luteus ifo 12708, and brev ... | 1979 | 120132 |
| [a strain of vibrio cholerae showing undergrowth on tcbs agar (author's transl)]. | | 1979 | 120392 |
| common protective antigen between pseudomanas aeruginosa and vibrio cholerae. | immunization with a common antigen (oep) of pseudomonas aeruginosa produced a high level of protection in mice against vibrio cholerae infection. the average oep-ha titer in mice sera of immune group was 1,600 ha titer. however, vibriocidal titer and agglutinin titer against v. cholerae were not observed in sera of mice immunized with oep. by passive administration of anti-oep rabbit serum, mice were also protected against challenge with v. cholerae. in order to confirm the presence of the commo ... | 1979 | 120454 |
| exotic virus infections of the liver. | | 1979 | 120555 |
| immunization against experimental trypanosoma cruzi infection: inherent difficulties of a uniform comparative evaluation of antigenic preparations. | | 1979 | 120626 |
| [examinations on the behaviour of grampositive and gramnegative bacteria in aqua bidest and tap-water at different initial colony counts and different temperatures (author's transl)]. | experiments were done about the behaviour of 5 different strains of staph. aureus, strept. faecalis, e. coli, ser. marcescens and p. aeruginosa in aqua bidest and sterile tap water at 20 degrees c, 30 degrees c and 40 degrees c. the behaviour of 3 different initial colony counts in each case was examined. colony forming units (cfu) were evaluated at the beginning and after 1, 2, 4, 6 and 24 h. cfu were reduced at staph. aureus and e. coli in aqua bidest and tap water not earlier than after 4 h, ... | 1979 | 120654 |
| [identification of a virus similar to hepatitis b virus in non-a non-b hepatitis]. | hepatitis b virus-like particles (including dane particles) with dna polymerase activity but negative for hbs ag have been identified in non-a, non-b hepatitis sera positive for hc ag. although specifically associated with the particles, hc ag is not a surface antigen of the hepatitis c virus identified here for the first time. the relationship of this agent with hbv seems obvious, and deserves further study. | 1979 | 120782 |
| [oculomotor reflex evoked by vibrassae stimulation in the cat]. | the oculomotor effects of vibrissae stimulation have been studied in the "encéphale isolé" non-anaesthetized cat. intracellular recording of lateral rectus motoneurones showed an excitatory projection via the ipsilateral infraorbital nerve to these motoneurones. the afferent fibers are type a alpha, recuited with a low threshold (0.2-0.5 ma) and convey information about vibrissae displacement. | 1979 | 120789 |
| enzymically-modified human mammary carcinoma cells: modulators of macrophage functions. | human mammary carcinoma cells (hmcc), vibrio cholera neuraminidase (vcn) treated hmcc(vcn-hmcc); glutaraldehyde fixed vcn-hmcc(glut-vcn-hmcc), normal mammary epithelial cells (hmec), vcn treated hnec (vcn-hnec) and glutaraldehyde fixed vcn-hnec (glut-vcn hnec) were prepared and injected into separate groups of mice. intraperitoneal injection of phytohemagglutinin (pha) produced a delayed type of inflammatory reaction characterized by an accumulation of macrophages. injection of hnec, vcn-hnec, a ... | 1979 | 121921 |
| responses to trh and t3 suppression tests in euthyroid subjects with a family history of graves' disease. | the relationship of graves' disease and heredity was studied in 97 clinically and biochemically euthyroid relatives (resin t3 uptake and serum t3, t4, and tsh within normal ranges) who had more than two thyrotoxic relatives within the second degree relationship. trh tests were preformed in all 97 cases. in 56 of the 97, t3 suppression tests were performed shortly after the trh test. results revealed that 29 of the 97 (29.9%) showed an abnormal response to trh. fourteen of these (14.4%) revealed ... | 1978 | 122407 |
| dynamic determinants of letf ventricular diastolic pressure-volume relations in man. | left ventricular (lv) diastolic pressure (p), volume (v), and rate of change of volume (dv/dt) were determined at 16.7 msec intervals in 17 patients (simultaneous micromanometer and single plane volume angiography). four patients had mitral stenosis with atrial fibrillation and 13 patients (three normal, two congestive cardiomyophathy, three lv hypertrophy, and five coronary artery disease) were in normal sinus rhythm. maximum early diastolic filling rates (max dv/dt) in the normal and cardiomyo ... | 1975 | 122921 |
| investigations on the biological activity of extracts of tylopilus felleus (bull. ex fr.) p karst. by means of mycological tests. | | 1975 | 123071 |
| inhibition of mitogen stimulation of human peripheral blood leukocytes by vibrio cholerae enterotoxin. | it has been found that the enterotoxin (choleragen) of vibrio cholerae, strain 569b, can interfere with phytohemagglutinin-p, pokeweed mitogen and concanavalin a stimulation of human peripheral blood leukocytes. the viability of cultured cells was not affected by the toxin. the toxin does not interfere with stimulation by direct competition for mitogen receptor sites. the ability of choleragen to inhibit stimulation by mitogens declines with time after initiation of stimulation. the biologically ... | 1975 | 123258 |
| ultrastructural studies on the myocardial capillaries of the experimentally lathyritic rat, protective effect of certain flavonoids. | the ultrastructure of rat myocardial capillaries was studied in the course of experimental lathyrism. endothelial cells were hypertrophic, with a sinuous profile of the plasma membrane facing the lumen and with a consistent increase of pinocytotic vesicles; the nuclei were irregular in shape; atpase activity was no more demonstrable. therefore, various and well distinct structural endothelial mechanisms seem to be primarily involved, causing an alteration of the dynamics of transcellular exchang ... | 1975 | 123669 |
| immunogenic properties of membrane-bound atpase from stable proteus p 18 l-forms : a kinetic study of inhibition by specific antibodies. | | 1975 | 123742 |
| enzymic synthesis of steroid sulphates. xi. study of the oestrogen binding site of oestrogen sulphotransferase by affinity labelling with 4-mercuri-17beta-oestradiol. | oistrogen sulphotransferase (3"-phosphoadenylylsulphate: oestrone sulphotransferase, ec 2.8.2.4) contains asingle sulphydryl group thought to be at, or near, the oestrogen-binding site. 4-mercuri-17beta-oestradiol, the activity of the enzyme decreased with increasing concentration of the oestrogen derivative. however, some 40% of the activity remained when all the sulphydryl had reacted to form mercaptide. formation of mercaptide was only marginally decreased in the presence of the substrate 17b ... | 1975 | 123774 |
| evidence that the deferential vein acts as a local transport system for androgen in the rat and the dog. | ligation of the deferential vein of the rat has been shown to bring about a reduction in the androgen-dependent activity of the prostatic rna polymerase enzyme. in the dog, the content of androgens in this vein is of the order of that found in the testicular vein and many times higher than that of peripheral plasma. it seems that the cauda epididymidis alone is sufficient to maintain the ipsilateral lobes of the prostate and seminal vesicles providing an intact ductus deferens and contralateral ... | 1975 | 123961 |
| fatal immune complex glomerulonephritis without deposits. | repeated intravenous injections of egg albumin in rabbits produced small antigen-excess complexes and severe glomerulonephritis. immunoglobulins and complement in the glomeruli were not clearly demonstrated by immunofluorescence; deposits were found to be sparse by electron microscopy. this study demonstrates that soluble immune complexes are responsible for the glomerular reaction. the apparent absence of deposits is thus not sufficient to exclude an immune complex pathogenesis. | 1975 | 123971 |
| effects of renal nerves on renal hemodynamics. i. direct stimulation and carotid occlusion. | renal nerves were stimulated either directly (loop electrodes) or indirectly (bilateral carotid occlusion with maintenance of control blood pressure) in anesthetized dogs to determine the effects on renal blood flow (rbf), glomerular filtration rate (gfr), sodium excretion (unav), and intrarenal distribution of blood flow (microsphere method). direct nerve stimulation decreased rbf 20% in 9 of 10 dogs; but gfr, unav, and intrarenal distribution of blood flow did not change significantly. carotid ... | 1975 | 124020 |
| hepatic mitochondrial function in ketogenic states. diabetes, starvation, and after growth hormone administration. | the study was designed to evaluate hepatic mitochondrial function during ketotic states. the ketogenic models studied were streptozotocin-induced diabetic ketoacidosis, 48 h of starvation, and after growth hormone administration. in the last-mentioned model we observed increased free fatty acids but not ketonemia. oxidative phosphorylation was measured using the citric acid cycle substrates pyruvate and succinate, the amino acid glutamate, a ketone body beta-hydroxybutyrate, and a long-chain fat ... | 1975 | 124319 |
| a comparison between the free grafting of sliced and intact muscles in the rat-1. | transverse slicing is a new technique whereby a mammalian muscle can be freely grafted with success. this method eliminates contamination of the early graft by surviving muscle fibres and allows one to measure the development of contractile properties on a uniform population of regenerating muscle fibres.?ioauthor | 1975 | 124664 |
| immunofluorescent localization of glycogenolytic and glycolytic enzyme proteins and of malate dehydrogenase isozymes in cross-striated skeletal muscle and heart of the rabbit. | specific antisera against glycogen phosphorylase, phosphofructokinase, aldolase, glyceraldehyde-phosphate dehydrogenase, enolase, lactate dehydrogenase, cytosolic and mitochondrial malate dehydrogenase from rabbit muscle were obtained from sheep. the gamma-globulins were used for indirect immunofluorescent localization of the respective enzymes in rabbit skeletal muscle and heart. in stretched skeletal muscle a cross-striation like distribution was observed for all enzymes studied. in the case o ... | 1975 | 124716 |
| inhibition not facilitation of sexual behavior by pcpa. | it has been proposed that estrous behavior in the female rat may be under tonic inhibition by a central serotonergi system. studies conbining estrogen priming and the pharmacological depletion of serotonin have provided some support for this hypothesis. some evidence, however, is not consistent with this hypothesis. in the present study estrogen primed ovariectomized-adrenalectomized rats were administered p-chlorophenylalanine and were tested for lordosis behavior 66 and 70 hr later. lordosis w ... | 1975 | 125426 |
| the effect of uncouplers on catecholamine incorporation by vesicles of chromaffin granules. | it is shown that uncouplers inhibit the incorporation of catecholamines by vesicles of chromaffin granules in parallel with their stimulatory effect on the membrane-bound adenosine triphosphatase. | 1975 | 125589 |
| a review of the role of swine skeletal muscle in malignant hyperthermia. | | 1975 | 125736 |
| response of frozen and stored canine lymphocytes in culture after thawing. | | 1975 | 125944 |
| protection against cholera. a bactericidal mechanism on the mucosal surface of the small intestine of mice. | | 1975 | 126054 |
| reversible and irreversible phases of serotonin depletion by 4-chloroamphetamine. | pretreatment of rats with an agent that inhibits uptake into serotoninergic neurons [lilly 110140: 3-(p-trifluoromethylphenoxy)-n-methyl-3-phenylpropylamine hydrochloride] prevented the depletion of brain serotonin by 4-chloroamphetamine, presumably by preventing the entry of 4-chloroamphetamine into the serotonin neuron. when the uptake inhibitor was given after 4-chloroamphetamine, the lowering of both serotonin and tryptophan hydroxylase levels in brain was reversed. serotonin levels returned ... | 1975 | 126166 |
| [clinical conference: hl-a antigens in chronic myeloid leukemia]. | | 1975 | 126439 |
| [clinical conference: histamine and basophils after cellular separation on albumin gradient]. | | 1975 | 126441 |
| immunochemical studies with synthetic immunogens chemically related to peptidoglycan. | rabbits were immunized with synthetic immunogens hsa-(gly-l-ala-l-ala-d-ala-d-ala)39 and hsa-(gly-gamma-d-glu-l-ala-d-ala-d-ala)40, respectively. antibodies against hsa-(gly-l-ala-l-ala-d-ala-d-ala)39 showed a strong precipitin reaction with the homologous antigen, with hsa-(gly-gamma-d-glu-l-ala-d-ala-d-ala)40 and with solubilized peptidoglycan containing peptide subunits with c-terminal d-alanyl-d-alanine. the albumin-peptide conjugates also cross-reacted with rabbit antisera to streptococcus ... | 1975 | 126550 |