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biosynthesis and function of gangliosides.gangliosides are unique acidic glycolipids that are selectively concentrated in the plasma membrane of cells. surface labeling studies have demonstrated that at least a portion of the oligosaccharde chain of gangliosides extends beyond the hydrophe) is imbedded in the membrane bilayer. it is becoming increasingly apparent that gangliosides participate in the internalization of environmental signals elicited by cholera toxin and glycoprotein hormones such as thyrotropic hormone and chorionic gona ...1976185697
stimulation of adenylate cyclase in washed pigeon erythrocyte membrane with cholera toxin and its subunits. 1976185963
subunit structure of cyclic gmp-dependent protein kinase from guinea pig fetal lung: dissociation of holoenzyme by cyclic gmp and histone. 1976186069
[effect of propranolol and trimepranol on the activity of carboxylic esterase, acid and alkaline phosphomonoesterases and adenosinetriphosphatase in the human thyroid gland]. 1976186198
the activation of adenylate cyclase by cholera toxin: possible interaction with the nucleotide regulatory site.the application of cholera toxin to intact cells causes a stimulation of adenylate cyclase activity. the effect is characterized by a lag period followed by a progressive rise in enzyme activity over several hours. only a few minutes' exposure to the toxin is required to produce effects lasting over several days. stimulation of adenylate cyclase by cholera toxin in broken cell preparations requires the presence of nicotinamide-adenine dinucleotide (nad) and an unidentified component of the cytos ...1976186240
adenylate cyclase in foetal rat testes and its stimulation by lh and naf. 1976186308
cyclic amp control by prostaglandin e1 in non-parenchymal liver cells. 1976186314
effect of propranolol on bile acid- and cholera enterotoxin-stimulated camp and secretion in rabbit intestine.stimulation of net secretion by deoxycholic acid (dca) in the colon and by cholera enterotoxin (ce) in the jejunum is mediated by camp. propranolol (pr) inhibits adenylate cyclase (ac) activity and net secretion induced by bile acid in the colon. the aim of this study was to assess the organ specificity of dca and ce as well as the selectivity of pr inhibition. three colonic and three jejunal loops were prepared in each of 8 rabbits treated intravenously with pr, 4 mg per kg, 1/2 hr before loop ...1977186351
effect of indomethacin on cholera-induced fluid movement, unidirectional sodium fluxes, and intestinal camp.cholera enterotoxin (ct) produces intestinal secretion associated with an elevation of intestinal cyclic amp (camp). indomethacin, a potent inhibitor of prostaglandin (pg) synthesis, decreases ct-induced secretion, although a role for pg in this process has not been demonstrated. the purpose of this study was to measure the effects of indomethacin on net fluid movement and unidirectional na fluxes in rabbit jejunal loops exposed to ct and to correlate these findings with intestinal camp levels. ...1977186352
histochemistry of oxidative enzymes in experimental allergic encephalomyelitis.the histoenzymic pattern of oxidative enzymes (g3pd, idh, sd, g6pd,hbd, nadph: dehydrogenase) was investigated in experimental allergic encephalomyelitis (eae) produced in rats according to paterson [13]. the results obtained lead to following conclusions: (1) the neuroglia, including the white matter oligodendroglia of immunized rats, exhibits increased oxidoreductase activities; (2) the neuroallergic reaction induces some stimulation of the oxidoreductive metabolism of oligoden-droglia; (3) th ...1976186370
microtubule assembly in cultivated greene melanoma cells is stimulated by dibutyryl adenosine 3':5'-cyclic monophosphate or cholera toxin.both dibutyryl cyclic amp (dbcamp) and cholera toxin promote the formation and elongation of processes of cultivated greene hamster melanoma cells. the formation and maintenance of these processes, which contain many microtubules, are sensitive to colcemid and vinblastine. tubulin was measured by [3h]colchicine binding and by acrylamide gel electrophoresis. we found that dbcamp or cholera toxin increases the ratio of polymerized to unpolymerized tubulin but not the total amount of tubulin per ce ...1976186462
plasma 16 beta-hydroxydehydroepiandrosterone in normal and pathological conditions in man.plasma 16beta-hydroxydehydroepiandrosterone (16 beta-oh-dhea) levels in normal subjects and patients with certain pathological conditions have been evaluated using radioimmunoassay of the steroid. plasma 16 beta-oh-dhea levels in normal subjects rose sharply during adolescence and then declined slowly throughout adult life: 192 +/- 54 (se) pg/ml between 7 and 11 yrs., 395 +/- 22 pg/ml between 15 and 19 yrs, 330 +/- 29 pg/ml between 20 and 39 yrs., 291 +/- 35 pg/ml between 40 and 59 yrs., and 124 ...1976186475
the release of endogenous amino acids from the rat visual cortex.the release of endogenous taurine, gaba, glycine, aspartate, glutamate, glutamine and alanine from the rat visual cortex was measured using a cortical cup technique. the electrocorticogram (ecog) was monitored throughout most experiments. 2. spreading depression, evoked by the dropwise placement of 10% kcl solution on to the brain outside the cup was associated with a significant increase in the release of gaba and glutamine but a marked fall in that of glutamate. the evoked release of gaba and ...1976186588
proteolytic enzyme activity in the granulation tissue of the human burn wound.a procedure for adsorbing enzymes from the human burn wound onto solid sheets of substrate is described. using this technique, low levels of enzyme activity with chymotrypsin-like specificity can be demonstrated in the wound approximately 2 weeks after injury. this activity disappears at about 5 weeks after the burn. the enzyme activity corresponds with the clinical experience for the time course of natural loss of the burn eschar. a trypsin-like enzyme of very low level activity is present in t ...1976186629
effects of cholera exotoxin on the mitochondrial morphology of lymphoid cells and mononuclear phagocytes. 1976186665
[behavior of certain parameters of lipid and energy metabolism. 3. relationships between cyclic 3',5'-adenosine monophosphate and acetyl coenzyme a in liver of growing rats deduced from model experiments with diets differeing in fat content].schemes of the lipid and the pyruvate metabolism serve to show that a great part of the enzymes which intervence in the metabolic pathways and are associated with the formation and the consumption of acetyl coenzyme a may be regulated by cyclic 3',5'-adenosine monophosphate (camp) in the sense of activation or inhibition. the camp increase in the liver, which has been demonstrated in the present study for a diet containing 25% of fat, opens the metabolic pathway to the formation of acetyl coenzy ...1976186712
[infections transmitted in swimming pools].public swimmingpools can be the source of infections due to micro-organism such as mycobacterium balnei, adeno and enteroviruses, the virus of plantar warts and molluscum contagiosum, the tric-agent of swimmingpool-conjonctivitis and pathogenic fungi. the transmission of trichomonas vaginalis is considered unlikely-water of pools, supposed to present satisfactory qualities by standard controls, was found to contain pathogenic staphylococci and pseudomonas aeruginosa. effective preventive measure ...1976186978
differential effects of prostaglandin synthetase inhibitors on prostaglandin e2 binding and on prostaglandin- or cholera toxin-induced cyclic amp accumulation in the rabbit uterus.cyclic 3',5'-nucleotide phosphodiesterase (pde) activity in rabbit uterine homogenate was inhibited by indomethacin (10 mug/ml; 66% inhibition) or flufenamic and (10 mug/ml; 60%). indomethacin (100 mug/ml) reduced uterine prostaglandin e2 (pge2) content by 80%, but potentiated the stimulatory action of purified cholera toxin (choleragen; 800%) and of exogenous pge2 (140%) on cyclic amp accumulation, probably through its inhibitory effect on cyclic amp destruction. these findings suggest that end ...1976187046
[subcspsular blastema of the adrenal cortex of the swine (sus scrofula domestica) after continuous acth long-term application]. 1976187085
transfer of parental immunity to infectious laryngotracheitis in chicks.the transfer of parental immunity to infectious laryngotracheitis was appraised by measuring serum antibody levels in 150 chicks from the day of hatch up to five weeks. the breeder flock which had received primary vaccination at eight weeks and a booster at 20 weeks transferred high antibody levels which fell markedly within two weeks and remained constant thereafter. chicks whose parents were vaccinated at 20 weeks only, had low antibody levels throughout. these low levels, in either group of c ...1976187298
(des-asp1) angiotensin i: a study of its pressor and steroidogenic activities in conscious rats.the steroidogenic and pressor activities of the nonapeptide (des-asp1) angiotensin i [(des-asp)-ai] were tested in conscious rats. (des-asp)-ai caused dose related increases in mean arterial pressure (map), serum aldosterone, and serum corticosterone in doses between 3 and 3,000 ng/kg/min. (des-asp)-ai was 14% as potent as angiotensin i and angiotensin ii and 60% as potent as (des-asp1) angiotensin ii [des-asp)-aii] in raising map. (des-asp)-ai was less active than ai, aii, or (des-asp)-aii in c ...1977187407
[effect of 2,6-diaminopurine resistance mutations on adenine and adenosine assimilation by cells of an adenine-dependent strain of escherichia coli k-12].in purine-requiring strain of escherichia coli k-12 defective in purine nucleoside phosphorylase (pur, pup) mutants (designated apt) have been obtained that are resistant to 2,6-diaminopurine on guanine-containing medium and incapable to utilize adenine for their growth at 42degreesc, but they are still sensitive to the analogue and can utilize adenine at 28degreesc. it has been shown that the introduction of the corresponding apt mutations in the genome of adenine-requiring strains impaired the ...1976187528
role of active sodium transport in brown fat thermogenesis. 1976187559
internally generated reduced nicotinamide adenine dinucleotide as a substrate for glycine transport by membrane vesicles of paracoccus denitrificans.internally generated reduced nicotinamide adenine dinucleotide was the most efficient substrate for glycine transport by membrane vesicles of paracoccus denitrificans.1977187573
spin-labeled stearates as probes for microenvironment of murine thymocyte adenylate cyclase-cyclic adenosine 3':5'-monophosphate system.the interaction of various spin-labeled compounds with the murine thymocyte adenylate cyclase-cyclic amp system was investigated. electron paramagnetic resonance spectra from spin-labeled compounds were used to calculate the order parameter, s, and indicated that the thymocyte plasma membrane is a relatively rigid structure. increasing concentrations of spin-labeled stearates, but not their corresponding methyl esters, resulted in increased membrane fluidity, partial lysis, and concomitant compl ...1976187588
hydrolysis of nicotinamide adenine dinucleotide by choleragen and its a protomer: possible role in the activation of adenylate cyclase.choleragen and the isolated a protomer catalyzed the hydrolysis of nad to adp-ribose and nicotinamide. the protein with nadase activity (nad nucleosidase; nad glycohydrolase, ec 3-2-2-5) migrated on polyacrylamide gels with choleragen, and chromatographed on bio-gel p-60 columns with the a protomer. the nadase activity of choleragen and of the a protomer was increased markedly in acetate and phosphate buffers, and enhanced over 10-fold by dithiothreitol in high concentration. nad hydrolysis was ...1976188038
effects of choleragen on hormonal responsiveness of adenylate cyclase in human fibroblasts and rat fat cells.choleragen increases cyclic amp content of confluent human fibroblasts. maximally effective concentrations of isoproterenol and prostaglandin e1 also induce large increases in cyclic amp content of human fibroblasts and in confluent cultures the effect of prostaglandin e1 is much greater than that of isoproterenol. after incubation with choleragen, the increment in cyclic amp produced by 2 mum isoproterenol is increased and approaches that produced by5.6 mum prostaglandin e1. although the concen ...1976188460
interaction of cholera toxin and its subunits with lymphocytes. the effects on intracellular camp. 1977188668
lytic action of polymyxin b on the liposomes prepared from vibrio cholerae phosphilipid. 1976188755
[cyclic nucleotides and intestinal function]. 1976189126
teratocarcinoma differentiation: plasminogen activator activity associated with embryoid body formation.changes in plasminogen activator activity have been examined as a clonal line of mouse embryonal carcinoma cells aggregate and differentiate to form cystic embryoid bodies in vitro. within the first 10 days of study, the pluripotent embryonal carcinoma cells aggregate; a layer of endodermal cells appears on the outside of the aggregate forming an embryoid body; a basement membrane forms between the outer layer of endodermal cells and the internal cells; a cyst forms within the embryoid body; and ...1977189937
role of cyclic am in steroidogenesis in leydig cells: discrepancies' between effects of luteinizing hormone and cholera toxin. 1977190030
intestinal secretion. 1977190182
differential effects of prostaglandin synthetase inhibitors on phosphodiesterase activity and choleragen-induced cycly amp production in rabbit uterus. 1976190187
[discreteness of satellite bacterial toxins]. 1977190828
ileal mucosal cyclic amp and cl secretion: serosal vs. mucosal addition of cholera toxin.changes in ion transport and cyclic amp (camp) concentration produced by addition of cholera toxin to the serosal side of isolated rabbit ileal mucosa (cts) were compared to the changes produced by addition to the mucosal side (ctm). cts increased short-circuit current (scc) as did ctm but it did so more slowly. cts, unlike ctm, did not significantly decrease electrical conductance. inhibition of the scc response to theophylline, a measure of preexisting secretion, was almost complete 180 min a ...1977190896
alteration in macromolecular glycosylation of transformed cells mediated by cholera toxin and dibutyryl adenosine 3':5'-cyclic monophosphate. 1976191314
effects of cholera exotoxin on fc receptor activity of lymphoid cells and mononuclear phagocytes.the effect of cholera exotoxin and aminophylline on fc receptors in a murine lymphoid-cell line and in rabbit pulmonary alveolar macrophages has been investigated. although both agents elevated intracellular cyclic amp levels in macrophages and lymphoid cells, the effects on fc receptor expression were distinct. cholera toxin at 10 microng/ml reversibly inhibited fc-receptor activity in the murine lymphoid cell line. in contrast, cholera toxin at 10 microng/ml or 0-01 microng/ml was ineffective ...1977191391
the number of receptors for beta-melanocyte stimulating hormone in cloudman melanoma cells is increased by dibutyryl adenosine 3':5'-cyclic monophosphate or cholera toxin.cultured cloudman melanoma cells exposed either to dibutyryl 3':5'-cyclic amp and theophylline or to cholera toxin bind significantly more 125i-labeled beta-melanocyte stimulating hormone (msh) and fluorescein-labeled msh than untreated cells. msh binds to melanoma cells in the g2 phase of the cell cycle. the stimulation of msh binding by dibutyryl cyclic amp results from an increase in the number of msh receptors per g2 cell and, to a lesser extent, from an increase in the number of g2 cells. t ...1977191818
cholera toxin requires oxidized nicotinamide-adenine dinucleotide to activate adenylate cyclase in purified rat liver plasma membranes.activation of adenylate cyclase in isolated rat liver plasma membranes by cholera toxin was demonstrated. the activation requires the presence of nad+ and atp and is irreversible.1977192202
synthesis and uptake of gangliosides by choleragen-responsive human fibroblasts.human fibroblasts, cultured in medium containing 10% fetal calf serum, responded dramatically to choleragen with an increase in cyclic adenosine monophosphate content to greater than 48 times basal levels. analysis of these cells for gangliosides indicated that the major ganglioside was n-acetylneuraminylgalactosylglucosylceramide (gm3) with trace amounts (less than or equal to 100 pmol/mg of protein) of other gangliosides including gm1, the putative choleragen receptor. although the cells conta ...1977192274
enzymatic and chemical oxidation of gangliosides in cultured cells: effects of choleragen.cell surface glycolipids of normal human fibroblasts and nctc2071 cells (transformed mouse fibroblasts) were labeled by incubating the intact cells with either galactose oxidase or sodium periodate, followed by reduction of the oxidized sugar residues with nab3h4. in intact human fibroblasts, incorporation of 3h was increased with increasing time of exposure to galactose oxidase prior to treatment with nab3h4. following limited exposure to galactose oxidase, more label was incorporated into the ...1977192275
arylamidases of rat liver and chemically induced hepatomas. ii. preparation and characterization of monospecific antisera against two distinct arylamidase-active antigens.monospecific antisera were prepared against the most prominent arylamidase (alpha-aminoacyl-peptide hydrolase (microsomal), ec 3.4.11.2) active antigen in plasma membranes (the plasma membrane arylamidase) and lysomal content (the lysosomal content arylamidase), respectively. plasma membrane extract and lysosomal content were allowed to react in crossed immunoelectrophoresis against their homologous antisera. the electrophoretic plates were washed extensively, dried and subsequently stained for ...1977192299
cholera toxin, ganglioside receptors and the immune response.cholera toxin activates plasma membrane adenylate cyclase in all mammalian cell types. the structure-function relationship of the toxin has recently been clarified, and the cell membrane receptor identified. this information has made cholera toxin the "agent of choice" for studies in many biological systems of the possible regulatory role of adenylate cyclase/cyclic amp. this article describes briefly our current knowledge about the toxin and its receptor. it then reviews recent research which h ...1976192668
glucose-induced proinsulin biosynthesis. role of islet cyclic amp. 1977193750
infectious murine type-c viruses released from human cancer cells transplated into nude mice.type-c virus particles were revealed by electron microscope in 6 of 9 tumours of cultured and biopsied human cancers heterotransplanted into nude mice. some tumours in nude mice were explanted for in vitro cultivation. the virus particles were also found in the cultures derived from the virus-positive tumors. they were mostly found extracellularly, but the particles in budding process were also encountered frequently. cytological study and karyotype analysis of the cultured cells proved these vi ...1977193756
the storage lipids in tangier disease. a physical chemical study.the physical states and phase behavior of the lipids of the spleen, liver, and splenic artery from a 38-yr-old man with tangier disease were studied. many intracellular lipid droplets in the smectic liquid crystalline state were identified by polarizing microscopy in macrophages in both the spleen and liver, but not in the splenic artery. the droplets within individual cells melted sharply over a narrow temperature range, indicating a uniform lipid composition of the droplets of each cell. howev ...1977193870
the role of cyclic nucleotides in lymphocyte activation and function. 1977194278
[characteristics and function of cholera toxin molecule (author's transl)]. 1976194285
modulation of in vitro erythropoiesis: enhancement of erythroid colony growth by cyclic nucleotides.mammalian erythropoiesis, as assayed by erythroid colony formation in vitro, is enhanced by cyclic adenosine nucleotides and agents which are capable of raising intracellular cyclic amp (camp) levels. with canine marrow cells as target, this enhancement was shown to be specific for camp and its mono- and dibutyryl derivatives. adenosine and its derivatives, such as amp, adp and atp, and other cyclic nucleotides, such as cgmp, dibutyryl-cgmp, ccmp and cimp and sodium butyrate were inactive. the p ...1977194698
fibrin adherent cho cell behavior in response to chelators and enterotoxin. 1977193707
regulation of norepinephrine release from cardiac sympathetic fibers in the dog by presynaptic alpha- and beta-receptors.the effect of phenoxybenzamine (pba), desmethylimipramine (dmi), clonidine (clnd), sotalol (stl), and isoproterenol (ispr) on the release of endogenous norepinephrine (ne) from the heart on right cardioaccelerator nerve stimulation was studied in anesthetized dogs. under control conditions, the catecholamine levels in coronary sinus blood increased linearly with increasing frequencies of stimulation up to 10 hz and did not increase further at 30 hz. the release of ne was markedly enhanced after ...1977193655
cerebrospinal fluid production: stimulation by cholera toxin.large increases in the production of cerebrospinal fluid have been observed after the intraventricular administration of cholera toxin. because cholera toxin stimulates adenylate cyclase, the data suggest that adenosine 3',5'-monophosphate plays a role in cerebrospinal fluid production.1977193189
cyclic adenylic acid-dependent and -independent production of chloramphenicol acetyltransferase in escherichia coli carrying r plasmids.a plasmid-specified, inducible, but cyclic adenylic acid (camp)-independent resistance to chloramphenicol (cm) is reported. the resistance level to cm was increased two- to fourfold by treatment with a small amount of cm for a short period in escherichia coli strains carrying the r plasmid pjy1, which was obtained from a clinical isolate of vibrio cholerae. though camp was required for production of cm acetyltransferase (catase) in camp-deficient mutants of e. coli carrying the r100 plasmid, the ...1977195517
the glucagon receptor and its relationship to adenylate cyclase. 1977194799
intercellular immunological controls and modulation of cyclic amp levels. some doubts.we have re-examined two sets of observations put forward to support the hypothesis that rises in camp levels induced by vasoactive amines and prostaglandins are involved in the intercellular control of immunological and inflammatory processes. (1) this hypothesis is said to be supported by the fact that cholera toxin, which raises camp levels in lymphoid tissue in vivo, is immunosuppressive. however, we found that cholera toxin inhibited antibody production only if given in doses causing gross d ...1976194828
postsynaptic induction of serotonin n-acetyltransferase activity and the control of cyclic nucleotide metabolism in organ cultures of the rat pineal. 1977196176
relationship between beta-adrenergic receptors and adenylate cyclase in hela cells. 1977196205
acute haemorrhagic conjunctivitis in hong kong 1971-1975.acute haemorrhagic conjunctivitis occurred in epidemic in hong kong in 1971 and 1975 respectively. two picornaviruses, the enterovirus type 70 and an antigenic variant of coxsackie virus type a24, were isolated during the 1971 outbreak, while only the latter virus was detected in the 1975 outbreak. in 1972 and 1974, sporadic cases of conjunctivitis associated with enterovirus type 70 were observed. serological survey in late 1975 showed a high proportion of the population might still be suscepti ...1977196351
correlation between changes in intracellular level of cyclic amp, activation of cyclic amp-dependent protein kinase, and the morphology of chinese hamster ovary cells in culture. 1977196552
transformation of chicken embryo retinal melanoblasts by a temperature-sensitive mutant of rous sarcoma virus.retinal melanoblasts were transformed by a temperature-sensitive mutant of rous sarcoma virus (ts-rsv). at the permissive temperature for transformation, the cells cease melanin synthesis, degrade their melanosomes and release much of their accumulated melanin into the medium. at the nonpermissive temperature, the cells assume an epithelioid morphology, actively synthesize melanin and become difficult to distinguish from normal uninfected control cultures. both the transformed phenotype and the ...1977196765
in vivo modulation of intracellular camp and cell growth of a lymphatic tumour in mice by cholera toxin. 1977196871
collagenase activity from cultured non-gravid rabbit uterus. 1977196959
effect of staphylococcus aureus delta toxin on chinese hamster ovary cell morphology and y-1 adrenal cell morphology and steroidogenesis.since staphylococcus aureus delta toxin previously had been shown to increase the cyclic adenosine 3',5'-monophosphate (camp) content of guinea pig ileum, the effect of delta toxin on such camp-mediated responses as morphogenesis and steroidogenesis in cultured tissue cells was examined. in contrast to cholera toxin, delta toxin did not cause spindling of chinese hamster ovary cells. unlike adrenocorticotropin or cholera toxin, delta toxin was unable to cause rounding of y-1 adrenal cells or to ...1977197006
mechanisms of disease and immunity in cholera: a review.the adenyl cyclase-activating enterotoxin of vibrio cholerae was shown to contain two types of subunit: six smaller units (l) that are responsible for the binding to cell membrane receptors and a larger unit (h) that mediates the toxic action. the receptor was identified as the ganglioside gm1 (galactosyl-n-acetylgalactosaminyl [sialosyl] lactosyl ceramide), and the results suggested that penetration of the toxin molecule into the membrane follows the rapid binding to gm1. the relationship of th ...1977197173
absence of the effect of ephedrine on levels of cyclic adenosine monophosphate in human lymphocytes.concentrations of 10(-6) to 10(-3) m ephedrine did not increase cyclic adenosine monophosphate (camp) levels of normal human lymphocytes even in the presence of theophylline or cortisol, which enhance the response to isoproterenol or prostaglandin e1. preincubation for 2 hr with ephedrine did not reduce the subsequent effect of isoproterenol, although preincubation with isoproterenol itself did. therefore, ephedrine is not a direct beta-adrenergic agonist on human peripheral blood lymphocytes.1977194936
regulation of amylase release from dispersed pancreatic acinar cells.1. a study has been made of factors influencing release of amylase from dispersed pancreatic acinar cells. 2. in the basal, unstimulated, condition cells released 2-3% of the total amylase present in 30 min. 3. the rate of amylase release was stimulated 50-70% by c-terminal octapeptide of cholecystokinin (cck-op, maximally effective concentration, 3 x 10(-10) m); carbamylcholine (maximally effective concentration, 10(-5 m); secretin (maximally effective concentration greater than 10(-6) m); vaso ...1977198531
substrate positions and induced-fit in crystalline adenylate kinase. 1977198550
cell receptors for paramyxoviruses.treatment of chick embryo fibroblasts, calf kidney and bhk cells for 30 minutes with the enzyme neuraminidase from vibrio cholerae causes an enhancement of the per cent of attached ndv virions. this enhancement does not depend on the multiplicity of infection. the quantity of spontaneously eluted and cellbound virus is two times greater than the quantity of the same virus derived from control cells. n-acetyl-neuramin lactose inhibits the effect of vibrio cholerae neuraminidase. after prolonged a ...1977199141
relationship between the beta-adrenergic receptor and adenylate cyclase. 1977195960
isolation and characterization of bound ion-sulfur proteins from bacterial photosynthetic membranes. i. ferredoxins iii and iv from rhodospirillum rubrum chromatophores. 1977199587
studies of isolated adult rat heart cells: the surface morphology and the influence of extracellular calcium ion concentration on cellular viability.rat cardiac muscle was dissociated into single cells by a coronary perfusion technique with collagenase and hyaluronidase in a ca-free medium. retention of the cylindrical shape of isolated muscle cells could be achieved by regulation of [ca2+]0 and temperature. cells kept at 4 degrees c, and 0-01 mm cacl2 remained cylindrical for more than a week and contracted spontaneously upon warming at 37 degrees c. at [ca2+]0 between 0-1-2 mm and 37 degrees c, cells underwent contracture and rounded up. s ...1977201046
progesterone interaction with estrogen and antiestrogen in the rat uterus--receptor effects.daily injections of estradiol or the antiestrogen tamoxifen initially stimulate uterine weight increase and progesterone receptor synthesis, though continued tamoxifen fails to maintain the increased weight. the stimulatory actions of both estradiol and tamoxifen are inhibited or reversed by a single injection of progesterone. it has been hypothesized that progesterone antagonizes estrogen action by reducing estrogen receptor levels, but in the present experiments neither cytoplasmic nor nuclear ...1977201053
[content of free amino acids in rabbit tissues as affected by adrenalectomy and administration of hydrocortisone and acth].adrenalectomy is accompanied by a decreased content of free amino acids in muscules and blood plasma by an increased content of certain amino acids in the liver. a single injection of hydrocortisone to adrenalectomized animals increases the content of amino acids in muscles and lowers their total content in the liver; a single injection of acth lowers the total content of amino acids in the liver and blood plasma "substitutive therapy" by hydrocortisone essentially increases the content of amino ...1977201065
viral hepatitis: enzyme assays and serologic procedures in the study of an epidemic.an epidemic of viral hepatitis beginning in late 1975 in a residence for multiply handicapped children, recognized very early in its course, was investigated prospectively to permit comparison of enzymatic and serologic tests. thirty-three residents of the institution and 46 full- and part-time employees were studied by the immune adherence hemagglutination procedure for antibody (anti-hav) to hepatitis a virus (hav). of these, 31 residents and 37 staff members were susceptible at the beginning ...1977201170
plasma adrenocorticotropin levels in normal dogs.plasma concentrations of adrenocorticotropin (acth; corticotropin) were measured in 31 normal dogs (house pets) at rest. the mean concentration was 45.77 pg/ml of blood, with individual values ranging from 17 to 98 pg/ml of blood. the measurement of this hormone secreted by the anterior lobe of the pituitary gland (adenohypophysis) may aid in the diagnosis of spontaneous canine adrenal cortical disorders, both primary and secondary.1977201191
cell culture propagation of porcine rotavirus (reovirus-like agent).two isolates of porcine rotavirus (reovirus-like agent) were isolated and passaged in primary procine kidney cell cultures. viral infectivity for cells was monitored by immunofluorescence because viral cytopathic effect was moderate. successful passage of virus in cell culture required that viral suspensions obtained from infected cell cultures be treated with pancreatin prior to inoculation onto cell monolayers. porcine rotavirus passage in cell culture also was accomplished, using trypsin trea ...1977201198
an investigation of the homology of guanylate kinase isozymes in mammals and further evidence for multiple guk gene loci.guanylate kinase in the red cells of 63 different mammalian species was studied by electrophoresis and multiple molecular forms of the enzyme were found in all species. two species were investigated in more detail. using molecular weight estimates as a criterion of homology, the fallow deer and the chinese hamster were found to have isozymes that corresponded to isozyme e, f, and g of man. variation in the guanylate kinase isozymes was detected in a small population of orangutans. results sugges ...1977201245
properties of a transposon conferring resistance to penicillins and streptomycin.r938 carries a transposon (tabeta) of approximate molecular weight 9.5 megadaltons (mdal, 10(6) daltons). this contains genes for a beta lactamase of type tem-1 and for streptomycin phosphatransferase (spt). there is a ten-fold difference in the efficiency of transposition in different strains of e. coli k12.1977201539
acth-dependent stimulation of a specific peptide in adrenocortical cells in culture. 1977197938
limbic system regulation of acth. 1977202143
laryngeal abductor activity during sleep. 1978202438
the effect of attapulgite and charcoal on enterotoxicity of vibrio cholerae and escherichia coli enterotoxins in rabbits.vibrio cholerae and certain strains of escherichia coli produce heat-labile enterotoxins which play a significant role in the pathogenesis of the intestinal disease. activated attapulgite, a heated magnesium aluminum silicate, was previously shown to prevent the toxic effects of endotoxin. the present study has revealed that this drug inhibits the toxic effects of cholera and e. coli enterotoxins in the intestinal loop of rabbits, when toxin and attapulgite are pre-incubated prior to injection. ...1977202565
structural changes of the tegumental membrane complex in relation to developmental stages of schistosoma mansoni (platyhelminthes: trematoda). 1977202726
interaction between cerulopasmin and sendai virus envelope components. note v. serological response in the rabbit to sendal virus and subviral fractions.immunization of rabbits with sendai virus and subviral fractions induced the appearance in the serum of both hemagglutinin- and neuraminidase-inhibiting antibodies. the study of the correlation between antigen characteristics and the level and kinetics of the antibodies suggests that a single antigenic glycoprotein carries both hemagglutinin and neuraminidase activities. the level of serum antibodies depends both on the size and structure of the antigens and on their organization, and reflects t ...1977203088
calcium gradient dependent pyrophosphate formation by sarcoplasmic vesicles.the vesicles of the sarcoplasmic membranes synthesize pyrophosphate from inorganic phosphate. pyrophosphate synthesis proceeds as long as a calcium gradient is maintained across the vesicular membranes. pyrophosphate synthesis is inhibited by low concentrations of nucleoside triphosphates.1977203132
multifunctionality of the enzyme in 2,3-bisphosphoglycerate metabolism of pig erythrocytes. 1977203152
increased de novo pyrimidine nucleotide synthesis in liver induced by ammonium ions in amounts surpassing the urea cycle capacity. 1977195808
actin-related gelation of ehrlich tumour cell extracts is reversibly inhibited by low concentrations of ca2+. 1978203867
the influence of crude cottonseed oil in the feed on the blood and egg yolk lipoproteins of laying hens.lipovitellin, very low density lipoproteins (vldl), low density lipoproteins (ldl), high density lipoproteins (hdl), and proteins of d greater than 1.20 were isolated from blood plasma and egg yolks obtained from hens fed a normal diet or one containing 2.5% of crude cottonseed oil. the amounts and compositions of each fraction were determined. hen blood plasma and egg yolk vldl and ldl obtained from hens fed a normal diet contained similar levels of lipid, and the fatty acid compositions of tho ...1977203909
in situ antigen localization with fluorescamine-conjugated antibodies: a new method. 1978204213
cytomegalovirus infection in the neonate and its prevention.about 0.5% of infants are infected in utero with cytomegalovirus (cmv). as many as 20% become mentally retarded and a further substantial proportion suffer lesser degrees of brain damage. the need for a vaccine is greater than in the case of rubella. a live, tissue culture-adapted strain of cmv has been shown to produce neutralizing antibody in volunteers without significant side effects or detectable virus excretion. the problems of developing such a vaccine for use in man, namely, attenuation, ...1977201930
correlation of type i, type ii, and reverse type i difference spectra with absolute changes in spin state of hepatic microsomal cytochrome p-450 iron from five mammalian species. 1978203579
latex agglutination test for amoebiasis in pakistani patients with chronic 'obscure' liver enlargements.the latex agglutination test for amoebiasis was done in 50 pakistani patients in whom a clinical diagnosis of chronic non-supparative amoebic hepatitis was made on the basis of liver enlargement associated with chronic recurrent abdominal disturbance. the serological test was negative in 32 (64%) and positive in 18 (36%). examination of stool specimens showed a higher frequency of e. histolytica in serology positive patients. the liver biopsy findings were variable and included normal histology ...1978204801
[viral diarrhea disease in calves]. 1978205036
effects of neuraminidase on lectin binding by wild-type and ricin-resistant strains of hamster fibroblasts.the nature of cell surface receptors for ricin on wild-type and ricin-resistant variants of baby hamster kidney fibroblasts has been studied. neuraminidase stimulated ricin binding threefold by wild-type cells, and increased their susceptibility to ricin toxicity as measured by inhibition of [3h]leucine uptake (ld30 fell from 5.0 to 0.5 microgram/ml). basal ricin binding by ricin-resistant variants (10-300% that of wild type) was also stimulated (2- to 17-fold) by neuraminidase in all seven clon ...1977199243
a light and electron microscopic study on complete dissociation of rat ascites hepatoma cells under activation of neutral protease and calcium depletion. 1978205995
determination of the inhibitory effect of several compounds on neuraminidases from virus influenza, v. cholerae and cl. perfringens. 1978204527
the common prohormone of corticotropin and the opiate peptide lipotropin c-fragment (beta-endorphin). 1978205469
effects of yunnan bai yao on blood platelets: an ultrastructural study.the effects of yunnan bai yao on blood platelets were studied at an ultrastructural level. this herbal drug is found to cause the release of platelet constituents which accounts for its hemostatic effects. the fine structural changes of platelets in response to this drug are also described.1977204448
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