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the sodium cycle: a novel type of bacterial energetics.the progress of bioenergetic studies on the role of na+ in bacteria is reviewed. experiments performed over the past decade on several bacterial species of quite different taxonomic positions show that na+ can, under certain conditions, substitute for h+ as the coupling ion. various primary na+ pumps (delta mu na+ generators) are described, i.e., na+ -motive decarboxylases, nadh-quinone reductase, terminal oxidase, and atpase. the delta mu na+ formed is shown to be consumed by na+ driven atp-syn ...19892687258
sodium-transport nadh-quinone reductase of a marine vibrio alginolyticus.the respiratory chain of a marine bacterium, vibrio alginolyticus, required na+ for maximum activity, and the site of na+ -dependent activation was localized on the nadh-quinone reductase segment. the na+ -dependent nadh-quinone reductase extruded na+ as a direct result of redox reaction. it was composed of three subunits, alpha, beta, and gamma, with apparent mr of 52, 46, and 32 kda, respectively. the reduction of ubiquinone-1 to ubiquinol proceeded via ubisemiquinone radicals. the former reac ...19892687259
respiratory na+ pump and na+-dependent energetics in vibrio alginolyticus.the marine bacterium vibrio alginolyticus was found to possess the respiratory na+ pump that generates an electrochemical potential of na+, which plays a central role in bioenergetics of v. alginolyticus, as a direct result of respiration. mutants defective in the na+ pump revealed that one of the two kinds of nadh: quinone oxidoreductase requires na+ for activity and functions as the na+ pump. the na+ pump composed of three subunits was purified and reconstituted into liposomes. generation of m ...19892687261
mechanism of lhrh-stimulated steroidogenesis in rat leydig cells: lipoxygenase products of arachidonic acid may not be involved.luteinizing hormone releasing hormone agonist, [(imbzl)-dhis6,pro9,net]-lhrh (lhrh-a), caused a two to threefold increase in in vitro testosterone (t) secretion by rat leydig cells. this lhrh-a-induced t secretion was completely blocked by quinacrine and chloroquine, inhibitors of phospholipase a2. addition of phospholipase a2, however, was ineffective in stimulating basal or lhrh-a-induced t secretion. phospholipase c, on the other hand, significantly stimulated both basal and lhrh-a-induced t ...19892695506
[existence of an additional structural component in the basal bodies of escherichia coli and vibrio alginolyticus flagellae].the structure of escherichia coli and vibrio alginolyticus flagella was studied using electron microscopy. an additional protein structure was shown to exist in the basal bodies of intact flagella in these organisms. it is possible that this structure involves three proteins important for the assembly of flagella, energy transduction, and a change-over in the direction of flagellar rotation.19892695799
[gypsum-bonded investments for the ringless casting (part 1). the mechanical properties of the experimental investments]. 20082699669
[gypsum-bonded investments for the ringless casting (part 1). the mechanical properties of the experimental investments]. 20082699669
non-synaptic release from dense-cored vesicles occurs at all terminal types in crayfish neuropile.in the crayfish neuropile, dense-cored vesicles (dcv) have been found in chemical terminals, mixed in with round or pleomorphic agranular synaptic vesicles, as well as in electrical terminals and neurohemal endings. dcv release their content at unspecialized non-synaptic sites. the simultaneous exocytosis of dcv and synaptic vesicles seems to be the rule in chemical terminals. dcv in specific terminals suggest non-synaptic communication. in chemical and electrical terminals, the content of dcv c ...20092702493
evaluating the optometric literature: the educated clinician.the purpose of this article is to present a framework for reader evaluation of optometric literature. the elements of a research article are outlined. suggestions are presented that clinicians can use to develop competence in evaluating research literature. also presented is an annotated bibliography of research references. better patient care and increased professional growth will result when clinicians evaluate and make use of the research literature.20082703662
evaluating the optometric literature: the educated clinician.the purpose of this article is to present a framework for reader evaluation of optometric literature. the elements of a research article are outlined. suggestions are presented that clinicians can use to develop competence in evaluating research literature. also presented is an annotated bibliography of research references. better patient care and increased professional growth will result when clinicians evaluate and make use of the research literature.20082703662
quantitative analysis of labelled inner retinal proteins in experimental optic neuritis.in order to determine if axonal transport changes in chronic experimental allergic encephalomyelitis (eae) were due to blockade or increased discharge of fast transported proteins from the inner retina, we examined the presence of pulse labeled proteins in autoradiograms of the optic nerve head, retinal ganglion cell and nerve fiber layers of juvenile strain-13 guinea pigs with chronic eae and normal controls. quantitative analysis of silver grains, performed six and twenty-four hours following ...19892707041
[children and domestic fights. a review of 105 social reports].the object of this investigation was to describe the conditions for children in families with domestic rows on the basis of 105 social reports made by the copenhagen police during a period of one year. a total of 162 children were involved in domestic rows. the typical family had 1-2 children. in 14% of the cases, the domestic rows were reported by the child. in connection with the domestic rows, violence to the women occurred in just under half of the cases and to the children in 6%. in 77% of ...20092711507
listening rate and comprehension as a function of preference for and exposure to time-altered speech.we conducted experiments on the effects of brief prior exposure to time-altered speech on preferred listening rate and the rate listeners would select when asked to listen to speech as fast as possible with good compression (induced listening rate). in exp. 1, 48 participants were exposed either to normal rate speech or to speech compressed to twice-normal rate. brief exposure to twice-normal rate speech led to a faster induced listening rate than exposure to normal rate speech. in exp. 2, 31 pa ...20092717361
[methods of capsular and intracapsular microsurgery, intracapsular implantation and sutureless fixation of artificial lenses].the author recommends mastering the methods of capsular and intracapsular microsurgery for cataracts on cadaver eyes. he advises to remove the cornea and the iris to have a better view of the technique. as the physician masters the technique, he screens the lens sac equatorial area by keeping the iris and later the cornea (to make the conditions as close to clinical surgery as possible) and then removes the iris and the cornea for controlling the quality of the capsular pocket preparation. artif ...20092718300
vibrio parahaemolyticus and other halophilic vibrios associated with seafood in hong kong.the summer prevalence of vibrio parahaemolyticus and other halophilic vibrios in seafood from hong kong markets was investigated. halophilic vibrios were isolated from all seven types of seafood examined, and comprised 9.1%, 8% and 6.1% of contaminating aerobic heterotrophic bacteria from mussels, clams and oysters respectively. sucrose-positive vibrios were more common than sucrose-negative varieties. vibrio alginolyticus was the most frequently isolated species, followed by v. parahaemolyticus ...19892722715
can the family physician avoid conflict of interest in the gatekeeper role? an affirmative view. 20002723596
malignant schwannoma. 20092724751
recurrent anorexia nervosa in a hypercholesterolemic woman treated with exercise and diet modification. 20092725596
[the first isolation of vibrio alginolyticus from samples which caused food poisoning].we report an event of food poisoning traced to eating salted shrimps. vibrio alginolyticus was shown to be the causative agent through epidemiological investigations and etiological tests. vibro alginolyticus can bring about human septicaemia and wound infection and it was found in the feces of patients with diarrhea, but no determination on its pathogenicity was done. from the samples of food which led to food poisoning. vibrio alginolyticus was isolated, and for the first time it was determine ...19892737046
ventricular arrhythmias and local electrograms after chronic regional denervation of the ischemic area in the pig heart.cardiac denervation has been proved to reduce the incidence of coronary occlusion arrhythmias in digs, but the effect of limiting the extent of sympathectomy to the ischemic area, particularly in hearts with sparse coronary collateral circulation, as in the human heart, needs further investigation. ventricular arrhythmias and changes in epicardial direct current electrograms induced during acute left anterior descending coronary artery occlusion were recorded in 14 pigs subjected to regional den ...19892738265
lack of nephrotoxicity of styrene at current tlv level (50 ppm).biochemical markers of kidney damage were examined in 37 female workers exposed to an average concentration of 225 mg/m3 of styrene. the concentration of mandelic acid in urine was on the average 759 mg/g creatinine. the mean duration of employment of the exposed subjects was 11 years. the results were compared to those obtained in 35 control female workers matched for age and a number of demographic and lifestyle factors and with no history of exposure to organic solvents. no difference was fou ...20092744872
terminal field of cholecystokinin-8-like immunoreactive projection neurons of the rat main olfactory bulb.the terminal field of cholecystokinin-8 (cck)-like immunoreactive (cck-ir) tufted cells in the rat main olfactory bulb was examined by means of immunohistochemistry combined with either an anterograde tracer or a degeneration method. cck immunostaining was carried out in animals in which phaseolus vulgaris agglutinin (pha) had been injected into the main olfactory bulb. pairs of adjacent sections were processed for cck and pha immunostaining, respectively. dense cck-ir terminallike staining was ...19892754048
predictive value of a short dietary questionnaire for changes in serum lipids in high-risk utah families.dietary questionnaires and serum cholesterol, triglycerides, and high-density lipoprotein (hdl) cholesterol determinations were completed for 1239 subjects aged greater than or equal to 20 at each of two separate screenings. the mean time between screenings was 2.5 y. after correcting for potential confounding variables, reduction of a measure of dietary cholesterol and saturated fatty acids assessed by two simple questions was a significant independent predictor of reduction in total cholestero ...20092756916
[microcomputer-assisted system for photographing the movements of biological valve prostheses].the aim of the study was to develop a clinically usable system of photographic imaging of the movements of biological valve transplants and of evaluating temporal and spatial relationships. the system has been established by means of a microcomputer and associated peripheral electronic equipment so that there is no need to use a high-speed camera. functioning of the system was verified by testing 37 aortal valve allotransplants and the björk shiley mechanical valve prosthesis.20092758334
aids: not the only infectious killer. 20092761978
studies in fetal wound healing: ii. a fetal environment accelerates fibroblast migration in vitro.we have used an in vitro model of wound healing using scratches made in a confluent monolayer of fibroblasts. the effects of fetal calf and postnatal calf serum on the migration of fibroblasts were compared. differences between fetal and calf serum-incubated fibroblasts grown on coverslips were observed within 15 minutes of exposure. cells in fetal serum began to change both shape and orientation and to move into the trough created by the scratch. the fibroblasts incubated in fetal calf serum co ...19892769548
studies in fetal wound healing: ii. a fetal environment accelerates fibroblast migration in vitro.we have used an in vitro model of wound healing using scratches made in a confluent monolayer of fibroblasts. the effects of fetal calf and postnatal calf serum on the migration of fibroblasts were compared. differences between fetal and calf serum-incubated fibroblasts grown on coverslips were observed within 15 minutes of exposure. cells in fetal serum began to change both shape and orientation and to move into the trough created by the scratch. the fibroblasts incubated in fetal calf serum co ...19892769548
[the rate of formation of necrotic focus and the severity of clinical picture in the acute period of myocardial infarction].the relationship between necrotic focus characteristics (weight and rate of necrotization) and the clinical pattern of myocardial infarction was examined. necrosis weight was shown to have no significant effect on the course of myocardial infarction, while maximum necrotization rate was found to be a valuable predictive factor: the higher the necrotization rate, the higher the risk of fatal outcome and various complications. prognosis, based on necrotization rate and patient's age, can be made, ...20092770081
anomalous origin of right pulmonary artery from the ascending aorta.anomalous origin of one pulmonary artery from the ascending aorta without associated intracardiac defects is a rare congenital malformation. about 60 anatomic and surgical descriptions have been reported in the literature. up to 1974, 22 of the 50 reported cases underwent surgical correction with a 40% mortality rate. the natural history without surgery is poor with a very high mortality during the first year of life. successful anatomic correction of anomalous origin of right pulmonary artery f ...20092777879
evaluation of media for monitoring fecal streptococci in seawater.the selectivity of kf streptococcus agar (kf) for monitoring fecal streptococci (fs) in seawater was examined in 234 samples of mediterranean water and compared with the selectivity of m-enterococcus agar (m-ent) for 124 samples and with bile-esculin-azide agar (bea) for 17 samples. kf was found to be unsuitable for marine water because vibrio alginolyticus and other gram-negative bacilli indigenous to this environment grew well on it and produced red colonies identical to those of fs. in 26% of ...19892782876
cetus banks on interleukin. 20092787891
[the ratios and kinds of clinical bacteria isolated in taiwan's large-size hospitals].the prevalence of clinical bacteria, as isolated from linko chang-gung memorial hospital (2,300 beds) in the period january 1985 to december 1986 and from taipei veterans general hospital (2,300 beds) during the period january 1986 to december 1986, was analyzed with the following findings: (i) the isolation ratio of anaerobic and aerobic or facultative bactria during the period of investigation were 7.8% (5,513/70,799) and 92.2% (65,286/70,799), respectively. (ii) of the total aerobic or facult ...19892791722
immune thrombocytopenia in aids patients: case report. 20092795303
solitary congenital nodular calcification of winer located on the ear: report of two cases.solitary congenital nodular calcification of the skin is a form of cutaneous calcinosis. it appears as a small, firm nodule on the head and extremities of young infants. the histogenesis of this condition is controversial. we cared for two patients with the nodule on the ear.20092798256
solitary congenital nodular calcification of winer located on the ear: report of two cases.solitary congenital nodular calcification of the skin is a form of cutaneous calcinosis. it appears as a small, firm nodule on the head and extremities of young infants. the histogenesis of this condition is controversial. we cared for two patients with the nodule on the ear.20092798256
organising clinical research: 2. writing the protocol. 20092803899
[the effect of progesterone, testosterone and estradiol on steroid hormone release by the ovarian follicles of cows in vitro]. 19892806660
retrospect and prospect of electron microscopy in japan: biology/virology. 19982809471
[intraoperative monitoring of cerebral function and carotid hemodynamics during carotid endarterectomy].the authors investigated the hemodynamics and monitored the cerebral function to perform the carotid endarterectomy (cea) safely. the hemodynamics were investigated by measurements of carotid arterial blood flow by an electromagnetic flow meter before and after cea. and a doppler flow meter applied directly to the carotid arteries to analyze the flow parameters such as peak frequencies (pf), mean frequencies (mf), mode frequencies (mo f) and % window. we used routinely our specially designed shu ...20092812257
[intraoperative monitoring of cerebral function and carotid hemodynamics during carotid endarterectomy].the authors investigated the hemodynamics and monitored the cerebral function to perform the carotid endarterectomy (cea) safely. the hemodynamics were investigated by measurements of carotid arterial blood flow by an electromagnetic flow meter before and after cea. and a doppler flow meter applied directly to the carotid arteries to analyze the flow parameters such as peak frequencies (pf), mean frequencies (mf), mode frequencies (mo f) and % window. we used routinely our specially designed shu ...20092812257
isotopic hybrids of nitrogenase. mössbauer study of mofe protein with selective 57fe enrichment of the p-cluster.previous mössbauer and epr studies of the mofe protein (approximately 30 fe and 2 mo) of nitrogenase have revealed the presence of two unique clusters, namely, the p-clusters (presumably of the fe4s4 type) and the molybdenum- and iron-containing cofactors (or m-clusters). mössbauer components d (approximately 10-12 fe) and fe2+ (approximately 4 fe) represent subsites of the p-clusters while component s (approximately 2 fe) appeared to belong to a separate, unidentified cluster. in order to refin ...19872820958
isotopic hybrids of nitrogenase. mössbauer study of mofe protein with selective 57fe enrichment of the p-cluster.previous mössbauer and epr studies of the mofe protein (approximately 30 fe and 2 mo) of nitrogenase have revealed the presence of two unique clusters, namely, the p-clusters (presumably of the fe4s4 type) and the molybdenum- and iron-containing cofactors (or m-clusters). mössbauer components d (approximately 10-12 fe) and fe2+ (approximately 4 fe) represent subsites of the p-clusters while component s (approximately 2 fe) appeared to belong to a separate, unidentified cluster. in order to refin ...19872820958
effect of cyclosporin a immunosuppression on primary lymphotropic herpesvirus infection in the guinea pig.female guinea pigs pretreated for 2 days with cyclosporin a (csa), were then inoculated intranasally (in) or intraperitoneally (ip) with a lymphotropic herpesvirus (gphlv) and followed by 4 additional daily doses of csa. immunosuppressed animals did not show lymphocytosis and virus infectivity titers in their spleen, cervical lymph node and blood mononuclear cells were lower than those of oil-treated controls. while in-inoculated csa-treated animals expressed higher virus infectivity titer in th ...19872832345
[biochemical aspect in cardiac ischemia--ischemic changes and reperfusion injury]. 20102838873
[restoration of the malar asymmetry with hydroxylapatite]. 20102856237
the na(+)-motive respiratory chain of marine bacteria.the respiratory chain of the marine bacterium vibrio alginolyticus pumps out na+ at the nadh:quinone oxidoreductase segment. the respiratory na+ pump plays an important role in the bioenergetics of this bacterium by generating a sodium-motive force as a direct result of respiration in alkaline na(+)-rich environments.19852856376
purification and regulation of glutamine synthetase in a collagenolytic vibrio alginolyticus strain.glutamine synthetase (ec 6.3.1.2) has been purified from a collagenolytic vibrio alginolyticus strain. the apparent molecular weight of the glutamine synthetase subunit was approximately 62,000. this indicates a particle weight for the undissociated enzyme of 744,000, assuming the enzyme is the typical dodecamer. the glutamine synthetase enzyme had a sedimentation coefficient of 25.9 s and seems to be regulated by adenylylation and deadenylylation. the ph profiles assayed by the gamma-glutamyltr ...19852859007
[improvement in the prognosis of rapid progressive glomerulonephritis by plasmapheresis treatment]. 19992860543
[current status of kidney transplantation and results]. 20012860545
blood lipid variability in relation to relative weight and biochemical markers of tobacco and alcohol consumption.carboxyhaemoglobin (cohb%) and gamma-glutamyl-transferase (ggt) are today frequently used as objective indicators of tobacco and alcohol consumption. the relationships between cohb%, ggt and relative body weight, cholesterol, triglyceride and apolipoprotein ai (apo-ai) were studied in middle-aged men attending a preventive medical programme in malmö, sweden. although statistically significant the influence of cohb% on cholesterol and triglyceride was found to be clinically insignificant. ggt and ...19852861602
membrane-linked energy transductions. bioenergetic functions of sodium: h+ is not unique as a coupling ion.the concept is developed according to which na+, like h+, can play the role of a coupling ion in energy-transducing biomembranes. this idea is based on observations that (i) na+ can be extruded from the cell by primary pumps (na-motive nadh-quinone reductase, decarboxylase or atpase), and (ii) the downhill na+ flux into the cell can be coupled with the performance of all the three types of membrane-linked work i.e. chemical (atp synthesis), osmotic (accumulation of solutes) and mechanical (motil ...19852863140
[two cases of insulinoma--comparison of histochemistry and immunoperoxidase method on imprint cytological slides to histological and ultrastructural findings]. 20102863406
[humoral factors of local and systemic immunity in the multiple peroral administration of a corpuscular pertussis vaccine].multiple oral immunization with pertussis corpuscular vaccine was shown to lead to the considerable stimulation of local and systemic humoral immunity. the data on the titers of specific and normal secretory antibodies, on the levels of iga in washings from the oral cavity, the small intestine and the lungs, on the titers of agglutinins and hemagglutinins in the blood serum, as well as on the morpho-functional transformation of the mucous membrane and the associated lymphoid tissue in the digest ...19852868593
[humoral factors of local and systemic immunity in the multiple peroral administration of a corpuscular pertussis vaccine].multiple oral immunization with pertussis corpuscular vaccine was shown to lead to the considerable stimulation of local and systemic humoral immunity. the data on the titers of specific and normal secretory antibodies, on the levels of iga in washings from the oral cavity, the small intestine and the lungs, on the titers of agglutinins and hemagglutinins in the blood serum, as well as on the morpho-functional transformation of the mucous membrane and the associated lymphoid tissue in the digest ...19852868593
vasovagal syncope? 20102869309
radioactivity in breast milk after chernobyl. 19862872450
temperature and oxygen regulated expression of a glutamine synthetase gene from vibrio alginolyticus cloned in escherichia coli.glutamine synthetase (gs) synthesis in vibrio alginolyticus was regulated by temperature, oxygen and nitrogen levels. a gs gene, glna from v. alginolyticus was cloned on a 5.67 kb insert in the recombinant plasmid prm210, which enabled escherichia coli glna, ntrb, ntrc deletion mutants to utilize (nh4)2so4 as a sole source of nitrogen. the v. alginolyticus glna gene was expressed from a regulatory region contained within the cloned fragment. v. alginolyticus glna expression from prm210 was subje ...19862880573
chernobyl and cancer epidemiology. 20092882249
somatostatinoma of vater's papilla and of the minor papilla.two cases of somatostatinomas of the major and minor papilla are reported. the tumors were characterized by solid trabecular and tubular arrangements of tumor cells and in one case by the presence of microvilli and psammoma bodies. the tumor cells contain many amine precursor uptake and dicarboxylation (apud)-granules with a diameter of 300 to 500 nm and low electron density. immunoperoxidase stain showed intense staining only for somatostatin and low density of neuron-specific enolase. those tu ...20102885079
testis position and age at orchidopexy. 20082885626
comparative study of the peripheral and central effects of terfenadine and cetirizine 2 hcl.the histamine cutaneous reactivity of healthy volunteers was measured after a single oral intake of placebo, terfenadine 60 mg and 180 mg, and cetirizine 2 hcl 10 mg. central side effects were evaluated by visual analog scales for drowsiness and movement coordination. the anti-h1 cutaneous effect of cetirizine 2 hcl proved to be significantly more rapid, more pronounced, and longer lasting than that of terfenadine 60 mg. cetirizine and terfenadine 180 mg were equipotent. the visual analog scales ...20102888424
evidence of functional alpha 2-adrenergic receptors in adult-rat adipocytes by using the agonist uk 14304.the aim of this study was to re-assess whether alpha 2-adrenergic receptors were present in rat adipocytes, by using uk 14304, a new and very selective alpha 2-agonist. the following observations demonstrate the presence of functional alpha 2-adrenoceptors in rat adipocytes. (1) adipocyte lipolysis was dose-dependently inhibited by uk 14304 (maximal effect 80% at 1 microm-uk 14304, 45% at 10 microm-uk 14304, under basal or theophylline-stimulated conditions respectively). (2) uk 14304 bound spec ...19882901830
renal metabolism of the oxidized form of ascorbic acid (dehydro-l-ascorbic acid).we evaluated whether specific transport and metabolic properties exist in rat and guinea pig kidney for handling the immediate oxidative product of ascorbic acid, dehydro-l-ascorbic acid. isolated tubules were used to measure uptake of 10 microm [14c]-dehydro-l-ascorbic acid over an 8-min incubation period. uptake did not show dependence on the bathing media electrolyte composition but was inhibited to some extent by glucose. in tubules of both animal species the majority of 14c label present in ...19892912166
toward 9 by '90: reducing infant mortality. 20102913170
individual microglia move rapidly and directly to nerve lesions in the leech central nervous system.small cells called microglia, which collect at nerve lesions, were tracked as they moved within the leech nerve cord to crushes made minutes or hours before. the aim of this study was to determine whether microglia respond as a group and move en masse or instead move individually, at different rates, and whether they move along axons directly to the lesion or take another route, such as along the edges of the nerve cord. cell nuclei in living nerve cords were stained with hoechst 33258 dye and o ...20102915975
dice: nonclinical causes of overtreatment. 20102916481
preventing second-generation complications due to staphylococcus aureus. 19892919929
sympathetically-induced development of tension in jaw muscles: the possible contraction of intrafusal muscle fibres.in rabbits, cats and rats anaesthetized, curarized, with the skull fixed in a stereotaxic apparatus, the peripheral stump of the cervical sympathetic nerve (c.s.n.) was electrically stimulated at frequencies within the physiological range and the isometric tension was recorded at the lower jaw. in a group of experiments the afferent discharge from the jaw elevator muscle spindles was also recorded, in the mesencephalic nucleus of the fifth cranial nerve. unilateral stimulation of the c.s.n. indu ...19852934686
differential depression of hypoglossal nerve activity by alcohol. protection by pretreatment with medroxyprogesterone acetate.upper airway obstruction during sleep occurs more commonly in men than in women and has been treated with progestational agents with some success. alcohol ingestion exacerbates sleep apnea, and recent studies have established that alcohol depresses the respiratory motor activity to upper airway muscles more than that to the diaphragm, a response pattern that favors upper airway obstruction during inspiration. to investigate the possibility that progesterone provides some protection from this act ...19862935056
the sodium cycle. ii. na+-coupled oxidative phosphorylation in vibrio alginolyticus cells.the role of na+ in vibrio alginolyticus oxidative phosphorylation has been studied. it has been found that the addition of a respiratory substrate, lactate, to bacterial cells exhausted in endogenous pools of substrates and atp has a strong stimulating effect on oxygen consumption and atp synthesis. phosphorylation is found to be sensitive to anaerobiosis as well as to hqno, an agent inhibiting the na+-motive respiratory chain of v. alginolyticus. na+ loaded cells incubated in a k+ or li+ medium ...19862942186
loss of complement receptor type 1 (cr1) on ageing of erythrocytes. studies of proteolytic release of the receptor.complement receptor type 1 (cr1) is a glycoprotein of mr about 250 000 present on erythrocytes and other cell types. cr1 acts as a cofactor in the factor i-mediated breakdown of complement fragment c3b to form ic3b. using an assay of cofactor activity, a wide variation in mean cr1 levels between erythrocytes from individual donors is observed. cr1 levels also decrease on ageing of erythrocytes in vivo, and again the rate of loss is widely variable between individuals. however, variable loss of c ...20102944510
interactions of monomeric igg bearing covalently bound c3b with polymorphonuclear leucocytes.the interactions of monomeric igg, monomeric c3b, dimeric c3b, and c3b covalently bound to igg (c3b-igg) with neutrophils were examined. c3b-igg heterodimers exhibited an increased affinity of binding to neutrophils, relative to c3b, both at half normal ionic strength (five-fold enhancement) and at normal ionic strength (six-fold enhancement). binding of monomeric igg to neutrophils was barely detectable and did not permit direct measurements of affinity in our assay conditions. the increased af ...20102953672
the atp-driven primary na+ pump in subcellular vesicles of vibrio alginolyticus.subcellular vesicles of vibrio alginolyticus hydrolyze atp and accumulate na+ in an atp-dependent fashion. the na+ uptake is (i) strongly stimulated by delta psi-discharging agents, i.e., the protonophorous uncoupler cccp or valinomycin + k+ and (ii) arrested by dccd at a concentration strongly inhibiting atp hydrolysis. lower concentrations of dccd stimulate the na+ accumulation supported by atp hydrolysis as well as by nadh oxidation. it is concluded that there is an electrogenic dccd-sensitiv ...19882968282
oxidative metabolism of neonatal and adult rabbit lung macrophages stimulated with opsonized group b streptococci.we compared the oxidative metabolism of alveolar macrophages (am) from adult and neonatal (1- and 7-day-old) rabbits before and after their in vitro exposure to type ia group b streptococci (gbs) opsonized with immune rabbit serum. nonstimulated am from 1-day-old, 7-day-old, or adult rabbits consumed o2 at a rate of 17 to 20 nmol/10(6) am per 10 min under basal conditions and released minimal amounts of superoxide (o2-) into the medium. approximately 80% of this basal respiration was of mitochon ...19852981197
the biologic significance of the mixed lymphocyte kidney culture in humans.the mixed lymphocyte kidney culture (mlkc) in humans has been studied in normal and abnormal clinical conditions. human renal cortical cells were extracted by collagenase treatment from the kidneys of "normal" heart-beating cadaver organ donors (n = 13), patients with end-stage renal disease (esrd) at pretransplant bilateral nephrectomy and splenectomy (n = 13), and from irreversibly rejected renal allografts at the time of graft nephrectomy (n = 5). proliferation of peripheral blood t lymphocyt ...19852996186
[role of virus-induced autoreactive t-lymphocytes, t-suppressors and the serum factor regulating their activity in the pathogenesis of experimental infection caused by the langat virus in mice].the precursors of autoreactive t-lymphocytes (partl) have been detected in the spleen of mice infected with langat virus. when introduced into syngeneic recipients, partl differentiate in their lymph nodes into autoreactive t-lymphocytes (artl) causing a fatal autoimmune disease in the syngeneic recipients in vivo and capable of destroying syngeneic cell cultures in vitro. in the thymus of mice infected with langat virus t-suppressors (ts) inhibiting the differentiation of partl into artl have b ...19852998127
generation of na+ electrochemical potential by the na+-motive nadh oxidase and na+/h+ antiport system of a moderately halophilic vibrio costicola.cells of vibrio costicola at ph 8.5 generate both membrane potential (inside negative) and delta ph (inside acidic) in the presence of a proton conductor, carbonyl cyanide m-chlorophenylhydrazone (cccp). the generation of cccp-resistant membrane potential was inhibited by 2-heptyl-4-hydroxyquinoline-n-oxide that is known to inhibit the na+-motive nadh oxidase of vibrio alginolyticus. nadh oxidase, but not lactate oxidase, of inverted membrane vesicles prepared from v. costicola required na+ for ...19863005258
deletion mutants that affect expression of epstein-barr virus nuclear antigen in cos-1 cells after gene transfer with simian virus 40 vectors containing portions of the bamhi k fragment.we have identified sequences that affect the efficient expression of epstein-barr virus nuclear antigen (ebna 1) when the structural portion of its gene, found within the 2.9-kilobase-pair bamhi/hindiii fragment called ilf, is expressed from a simian virus 40 vector. a set of nested deletions at the bamhi end of the fragment was constructed by using bal 31 digestion, the addition of linkers, and ligation into psvod. the mutants were tested for their ability to express antigen in cos-1 monkey cel ...19863009849
plaque autoradiography assay for the detection and quantitation of thymidine kinase-deficient and thymidine kinase-altered mutants of herpes simplex virus in clinical isolates.a plaque autoradiography assay to detect and quantitate thymidine kinase (tk) mutants of herpes simplex virus type 1 (hsv-1) and hsv-2 in clinical samples is described. this method utilizes the selective incorporation of [125i]iododeoxycytidine, a pyrimidine analog selectively phosphorylated by the hsv tk. only cells infected with tk-competent virus will efficiently incorporate iododeoxycytidine and are the only cells detected by autoradiography. furthermore, this assay discriminates between tk+ ...19853010836
eeg interaction between delta 8-tetrahydrocannabinol and some sedative-anxiolytic drugs. is the anxiogenic effect of cannabis related to an action on the gabaergic system? 19863017070
evidence that a human soluble beta-galactoside-binding lectin is encoded by a family of genes.two cdna clones were isolated by immunoscreening a human hepatoma cdna library with an antiserum that bound specifically to a human soluble beta-galactoside-binding lectin with mr of approximately 14,000. the deduced amino acid sequences of the inserts of these two clones show considerable homology with each other, the sequence of chicken skin beta-galactoside-binding lectin, and eight peptides derived from purified human lung lectin of mr approximately 14,000. however, the sequence differences ...19863020551
evidence that a human soluble beta-galactoside-binding lectin is encoded by a family of genes.two cdna clones were isolated by immunoscreening a human hepatoma cdna library with an antiserum that bound specifically to a human soluble beta-galactoside-binding lectin with mr of approximately 14,000. the deduced amino acid sequences of the inserts of these two clones show considerable homology with each other, the sequence of chicken skin beta-galactoside-binding lectin, and eight peptides derived from purified human lung lectin of mr approximately 14,000. however, the sequence differences ...19863020551
[differential diagnosis of viral diarrheas in calves].there is a description of the enzymoimmunologic method (elisa), which was used for demonstration of rotaviruses and coronaviruses in the samples of excrements of the calves suffering from diarrheas. it is shown by a comparison with the results obtained by electron microscopy that the elisa method provides by up to 50% higher capture rate and the reaction is highly specific. the method can also be applied to a detection of human rotavirus in the children's stool.19863020762
[differential diagnosis of viral diarrheas in calves].there is a description of the enzymoimmunologic method (elisa), which was used for demonstration of rotaviruses and coronaviruses in the samples of excrements of the calves suffering from diarrheas. it is shown by a comparison with the results obtained by electron microscopy that the elisa method provides by up to 50% higher capture rate and the reaction is highly specific. the method can also be applied to a detection of human rotavirus in the children's stool.19863020762
enhanced expression of the c-myc gene in bovine leukemia virus-induced bovine tumors.we have detected elevated levels of c-myc gene expression in neoplastic cells from all seven bovine leukemia virus (blv)-induced bovine tumors examined, but not in blv-infected, nonneoplastic lymphoid cells. no rearrangement or amplification of the c-myc gene could be demonstrated in any of the blv-induced tumors. furthermore, blv proviral dna was found to have no preferred site of integration in these tumors. the possible mechanisms of enhanced expression of the c-myc gene in blv-induced tumors ...19863022917
purification and characterization of a streptomycete collagenase.a soil streptomycete designated as streptomyces sp. a8 produced an extracellular collagen hydrolysing enzyme that appeared to be 'true collagenase' as it degraded native collagen under physiological conditions and cleaved the synthetic hexapeptide 4-phenylazobenzyloxycarbonyl-l-prolyl-l-leucyl-glycyl-l-prolyl-d-a rginine into two tripeptides. the enzyme was purified by diethyl aminoethyl cellulose chromatography and sephadex g-150 gel filtration. the purified enzyme had an apparent molecular wei ...19863023269
familial polyposis coli in childhood.familial polyposis coli has generally been considered a disorder of adulthood that is rarely identified in children. we report 3 affected kindreds in which the disorder was diagnosed in 6 of 11 potentially affected children between 8 and 16 years of age. experience with these kindreds demonstrates that polyps frequently develop during childhood in affected individuals. colonoscopy was found to be preferable to the air-contrast barium enema, in that it was as sensitive a technique for detection o ...20103028683
familial polyposis coli in childhood.familial polyposis coli has generally been considered a disorder of adulthood that is rarely identified in children. we report 3 affected kindreds in which the disorder was diagnosed in 6 of 11 potentially affected children between 8 and 16 years of age. experience with these kindreds demonstrates that polyps frequently develop during childhood in affected individuals. colonoscopy was found to be preferable to the air-contrast barium enema, in that it was as sensitive a technique for detection o ...20103028683
seasonal changes in the cecal microflora of the high-arctic svalbard reindeer (rangifer tarandus platyrhynchus).the dominant cecal bacteria in the high-arctic svalbard reindeer were characterized, their population densities were estimated, and cecal ph was determined in summer, when food quality and availability is good, and in winter, when it is very poor. in summer the total culturable viable bacterial population was (8.9 +/- 5.3) x 10(8) cells ml-1, whereas in winter it was (1.5 +/- 0.7) x 10(8) cells ml-1, representing a decrease to 17% of the summer population density. of the dominant species of cult ...19873030193
seasonal changes in the cecal microflora of the high-arctic svalbard reindeer (rangifer tarandus platyrhynchus).the dominant cecal bacteria in the high-arctic svalbard reindeer were characterized, their population densities were estimated, and cecal ph was determined in summer, when food quality and availability is good, and in winter, when it is very poor. in summer the total culturable viable bacterial population was (8.9 +/- 5.3) x 10(8) cells ml-1, whereas in winter it was (1.5 +/- 0.7) x 10(8) cells ml-1, representing a decrease to 17% of the summer population density. of the dominant species of cult ...19873030193
evolution of localization of reactions of adenosine triphosphatase (mg++-atp-ase), 5'nucleotidase (5'nt), alkaline phosphatase (ap), and acid phosphatase (acp) in developing rat testis. ii. after cdcl2 treatment.the described experiments show the influence of a single dose of cadmium chloride (1.5 mg cdcl2/kg body weight applied intraperitoneally) on development of the male gonads from the 1st d of post-fetal life to 1.5 a of life. in the case of the enzymes triggering transportation processes, adenosine triphosphatase stimulated by mg++ (mg++-atp-ase), alkaline phosphatase (ap), and 5'nucleotidase (5'nt), a considerable damages begin to appear in the 15th d of life whereas in the case of acid phosphata ...19873031915
role of postnatal gonadal function in the determination of thyrotropin (tsh) releasing hormone-induced tsh response in adult male and female rats.permanent effects of postnatal gonadal function on the hypothalamo-pituitary-thyroid axis were examined in male and female rats of the wistar-imamichi strain. animals were used at the age of about 10 weeks. neonatally castrated (nc) males showed a significantly higher plasma tsh response to trh (10 micrograms/kg bw, ip) than males castrated at 1, 2, 3, 4, or 6 weeks of age. the tsh response in intact males was similar to that seen in nc males. neonatal androgenization of nc males with 100 microg ...19873032567
expression and regulation of a vibrio alginolyticus sucrose utilization system cloned in escherichia coli.a halotolerant collagenolytic vibrio alginolyticus strain isolated from salted hides had intracellular sucrase activity and did not secret sucrase into the medium. the strain actively transported sucrose by a sucrose-inducible, na+-independent process. a 10.4-kilobase dna fragment of v. alginolyticus dna was cloned into escherichia coli. the recombinant e. coli(pvs100) could utilize sucrose as a sole carbon source. in contrast to v. alginolyticus, the recombinant e. coli produced both intra- and ...19873034863
dot assay for neomycin phosphotransferase activity in crude cell extracts.a dot assay for determining neomycin phosphotransferase (npt ii) activity in crude cell extracts has been developed. the assay provides for the rapid screening of large numbers of cell cultures generated in gene transformation experiments using npt ii as a dominant selectable marker. currently, the commonly used procedure for npt ii assay employs a time-consuming electrophoretic protein separation step to eliminate a positive interference resulting from putative protein kinase activities present ...20113037946
expression and secretion in yeast of a 400-kda envelope glycoprotein derived from epstein-barr virus.the major envelope glycoprotein (gp350) of epstein-barr virus has been expressed and secreted in the yeast saccharomyces cerevisiae as a 400-kda glycoprotein. this is the first example of the secretion of such a large, heavily glycosylated heterologous protein in yeast. since gp350 proved highly toxic to s. cerevisiae, initial cellular growth required repression of the expression of gp350. using temperature- or galactose-inducible promoters, cells could be grown and the expression of gp350 then ...19873038696
the effects of in vivo antibiotics on neutrophil (pmn) activity in rabbits with peritonitis.antibiotics play an important role in helping the host fight infection; however, the direct cellular effect of antibiotics on polymorphonuclear cells remains undefined. adherence, chemotaxis, phagocytosis, and superoxide anion production are important steps in the cascade of events initiated by the polymorphonucleocyte in bacterial killing. previous studies have shown inhibition as well as stimulation of neutrophil antibacterial therapy by antibiotics. peritoneal and blood polymorphonuclear neut ...19873041106
[x-ray computed tomography in venous pathology].after presenting the tomodensitometric semiology of venous thromboses in various territories, the authors define its indications. it is essentially in deep territories, head, chest and abdomen that the ct-scan is used to study and diagnose unrecognized thromboses as well as determine the venous repercussion of various neighboring ailments. the respective place of the ct-scan, ultrasonography and various phlebographies is analyzed.20113043474
sensitivity of some marine bacteria, a moderate halophile, and escherichia coli to uncouplers at alkaline ph.the inhibitory effects of uncouplers on amino acid transport into three marine bacteria, vibrio alginolyticus 118, vibrio parahaemolyticus 113, and alteromonas haloplanktis 214, into a moderate halophile, vibrio costicola nrc 37001, and into escherichia coli k-12 were found to vary depending upon the uncoupler tested, its concentration, and the ph. higher concentrations of all of the uncouplers were required to inhibit transport at ph 8.5 than at ph 7.0. the protonophore carbonyl cyanide m-chlor ...19883045092
similarity between active and trypsin-activated inactive renin in dog plasma by means of renin inhibition: the dog as an animal model for studies of inactive renin.a method for trypsin-activation of dog plasma inactive renin is described. liquid phase trypsin (final concentration 6.7 mg/ml) was used and the reaction was stopped after 2 min at 4 degrees c by soybean trypsin inhibitor (13 mg/ml). renin was measured as angiotensin i (ang i) generation in trypsin-treated and untreated plasma using the antibody-trapping method, in the presence of excess ox renin substrate. the renin-like activity after trypsin was indeed due to renin, since ang i generation in ...19883065412
[the 35th anniversary of the independent existence of czechoslovak pharmacy schools]. 20113072093
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