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n-ethylmaleimide desensitizes ph-dependence of k+/h+ antiporter in a marine bacterium, vibrio alginolyticus.the k+/h+ antiporter of a marine bacterium, vibrio alginolyticus, is strongly dependent upon the cytoplasmic ph and functions only at an internal ph above 7.7. in alkaline buffer with an outwardly directed chemical gradient of k+ (delta pk), the internal ph was maintained at about 7.7. addition of n-ethylmaleimide (nem) released cellular k+ and acidified the cytosol below ph 7.7. the nem effect was reversed by the addition of 2-mercaptoethanol: k+ efflux ceased, and the internal ph returned to a ...19863718495
progesterone regulation of secretory component (sc): uterine sc response in organ culture following in vivo hormone treatment.sex steroid hormones are known to have profound effects on mucosal immunity. in the present study we evaluated the effects of progesterone on the uterine immune system by determining the changes in the levels of secretory component (sc) released from uterine tissues in culture following in vivo administration of progesterone to estradiol-stimulated ovariectomized rats. sc is a transport protein which moves iga into external secretions such as intestinal and uterine secretions. sc release was det ...19883386246
purification and initial characterization of the 71-kilodalton rat heat-shock protein and its cognate as fatty acid binding proteins.the major rat heat-shock (stress) protein and its cognate were purified to electrophoretic homogeneity from livers of heat-shocked rats. both proteins exhibited similar behavior on a variety of column chromatography matrices but were separable by preparative isoelectric focusing under nondenaturing conditions by virtue of a 0.2 ph unit difference in isoelectric point. both purified proteins had similar physical properties, suggesting the possibility that they may have similar biological function ...19863730359
the sodium cycle. iii. vibrio alginolyticus resembles vibrio cholerae and some other vibriones by flagellar motor and ribosomal 5s-rna structures.an electron microscopic study of the basal bodies of the vibrio albinolyticus flagellum revealed a four-disc structure. the diameters of the two discs localized closer to the cytoplasmic membrane proved to be about 2-fold shorter than those of the two others. in this respect the basal body of v. alginolyticus resembles very much that of v. cholerae described by ferris and co-workers. the sequence of the v. alginolyticus ribosomal 5s-rna showed that it is similar to those of v. cholerae, v. harve ...19863730372
effect of pre- and postnatal exposure to alcohol on perivenous and periportal neonatal rat hepatocytes.hepatocytes from 12-day-old rats, pre and postnatally exposed to alcohol and pair-fed controls, were isolated and subfractionated in six cell subpopulations on percoll density gradients. these cells were characterized using biochemical, stereological and cytofluorometric methods. our results show that the low density cells (f2) are mainly perivenous cells, whereas the heavier cells (f6) were primarily periportal cells. these results were confirmed by alanine aminotransferase (alat), and glutamat ...19873426691
development and characterization of a rat histiocyte-macrophage tumor line.a transplantable macrophage-like cell line has been obtained and established in w rats. this cell line is in its 85th passage and is perhaps the only established macrophage-like cell line that grows rapidly intraperitoneally in rats. the cells possess some of the typical characteristics of macrophages, such as adherence to glass, phagocytosis, presence of fc receptors and c3d receptors, la determinants, leukocyte common antigen, lysozyme, non-specific esterase, and glycogen. the tumor also grows ...19863457975
biotechnological applications of lipid microstructures. based on the proceedings of the workshop on technological applications of phospholipid bilayers, vesicles, and thin films, january 5-9, 1986, tenerife, canary islands; and the workshop on biotechnological applications of membrane studies, october 18-22, 1987, san sebastian, spain. 19883250236
the respiratory burst oxidase of human neutrophils. further studies of the purified enzyme.a superoxide-forming oxidase from activated human neutrophil membranes was solubilized by two slightly different methods, then purified by "dye-affinity" chromatography. kinetic studies of the purified preparations gave vmax values of 5-10 mumol of o-2/min/mg of protein, and km values for nadh and nadph that were in reasonable agreement with values determined previously using particulate and crude solubilized preparations of the respiratory burst oxidase. sodium dodecyl sulfate-polyacrylamide ge ...19863759962
migration of fish leucocytes in vitro: the effect of factors which may be involved in mediating inflammation.plaice (pleuronectes platessa l.) neutrophils were isolated from the kidney on a discontinuous percoll gradient and from the peritoneal cavity at the peak of a glycogen-elicited inflammatory response. the migratory ability of neutrophils was assessed using a 48-well microchemotaxis chamber, with an incubation of 1.5 h at 12 degrees c. the two neutrophil populations showed different responses to n-formylmethionyl-leucyl-phenylalanine (fmlp). whereas kidney neutrophils only showed a significant en ...19863765366
dose-dependent effects of oral and intravenous glucose on insulin secretion and clearance in normal humans.insulin secretion and clearance were studied in 2 groups of 7 normal subjects who each received 25, 50, and 100 g of glucose either orally or intravenously (iv) on separate occasions. insulin secretion rates were calculated during a 1-h base line and for 5 h after glucose administration from a two-compartmental analysis of peripheral c-peptide concentrations using individual kinetic parameters derived after iv bolus injections of biosynthetic human c-peptide. incremental glucose areas after oral ...19883279811
reversibility of decreased insulin-stimulated glucose transport capacity in diabetic muscle with in vitro incubation. insulin is not required.the mechanisms by which insulin deficiency affects muscle glucose transport were investigated. epitrochlearis muscles from rats with streptozotocin-induced diabetes and from controls were incubated in vitro for 0.5-14 h. the incubation was shown not to impair muscle energy stores or tissue oxygenation. diabetes decreased basal 3-o-methylglucose transport by 40% (p less than 0.01), and insulin-stimulated (20 milli-units/ml) glucose transport capacity by 70% (p less than 0.001). in vitro incubatio ...19873294836
occurrence and distribution of halophilic vibrios in subtropical coastal waters of hong kong.the summer occurrence and distribution of halophilic vibrios in the subtropical coastal waters of hong kong were investigated. the density of vibrios in six sample sites ranged from 90 to 6,700 per ml, which made up 0.41 to 40% of the total bacterial populations of these sample sites. the sucrose-positive vibrios were found to be much more common (88% of total vibrios) than the sucrose-negative ones. a total of 48 strains belonging to six vibrio species were fully characterized. among these, vib ...19863789725
immunological studies on crohn's disease. iv. decreased adcc activity.antibody dependent cell-mediated cytotoxicity (adcc) activity was studied in 16 japanese patients with crohn's disease (cd) and 16 healthy controls. adcc activity was assessed by measuring 51cr release from antibody coated human b-cell line. significantly (p less than 0.002) depressed adcc activity was found in patients with cd, although adcc activity was not directly related to the disease activity. to determine causative factors related to reductions in adcc activity, circulating immune comple ...19863485720
isolation of vibrio alginolyticus from two patients of acute gastroenteritis. 19863794263
effect of 1,25-dihydroxyvitamin d3 on insulin secretion: direct or mediated?in order to clarify the role of vitamin d (d) in regulating insulin secretion, we studied the effect of long term (10 days) and short term (3 days) supplementation with d and/or calcium (ca) on insulin secretion from the isolated, perfused pancreas of d- and ca-deficient rats. the influence of the nutritional state induced by d deficiency was also evaluated. the long term supplementation of either d, ca, or both restored the body weight and improved insulin secretion induced by high glucose conc ...19863516655
[use of methylumbelliferylglucuronide (mug) for the rapid identification of e. coli in food microbiology]. 19883072974
the effect of toluene inhalation exposure on catecholamine contents in rat sympathetic neurons.adult male rats were exposed to toluene in short-term exposure by inhalation for 48 h (2000 ppm, continuously), and in long-term inhalation for 3 months (1000 ppm, 8 h daily). the formaldehyde-induced fluorescence (fif) technique for histochemical demonstration of catecholamines (ca) was used to detect changes in the catecholamine stores. the concentration of ca in the sympathetic neurons of superior cervical ganglia and adrenal medulla was measured by the fif technique combined with microfluori ...19863807443
[diagnosis of borrelia infections is an all-year-round problem as the serological examination of the cases from 1985 shows]. 19863807483
[the role of na+ ions in the respiration, formation of the membrane potential and movement of the alkali-resistant marine bacterium vibrio alginolyticus].subbacterial vesicles capable of generating delta psi during nadh oxidation were obtained. the oxidation of nadh was stimulated by na+ and inhibited by 2-heptyl-4-oxyquinoline-n-oxide (hqno) in submicromolar concentrations. the generation of delta psi was inhibited by hqno in low concentrations, cyanide, gramicidine d and carbonyl cyanide-m-chlorophenylhydrazone (cccp) in combination with monensine. at the same time, in the absence of monensine cccp influenced the delta psi generation in a much ...19873814650
production of extracellular enzymes and cytotoxicity by vibrio vulnificus.thirty-three strains of vibrio vulnificus of clinical and environmental origin were examined for production of 12 extracellular enzymes of potential importance to the virulence of this bacterium. strains of vibrio vulnificus were consistent in their production of protease, mucinase, lipase, chondroitinase, hyaluronidase, dnase, sulfatase, and hemolysin. no differences between clinical and environmental isolates were noted. although none of the enzymes appeared to correlate with the ability of th ...19863522090
acquired sideroblastic anaemia after aplastic anaemia caused by d-penicillamine therapy for rheumatoid arthritis.a 68 year old man with rheumatoid arthritis developed marrow aplasia during d-penicillamine treatment. recovery of granulopoiesis and erythropoiesis was ineffective with features of a secondary sideroblastic anaemia. absence of megakaryopoiesis persisted. therapeutic measures failed, and the patient finally died. these events illustrate a haematopoietic stem cell injury induced by d-penicillamine.19873827338
effect of opsonization on oxidative metabolism of plaice (pleuronectes platessa l.) neutrophils.the effect of serum opsonization on vibrio alginolyticus (heat-killed)-stimulated chemiluminescence (cl) by plaice kidney- and peritoneal exudate-derived neutrophils was investigated. peritoneal neutrophils only recognized heat-labile and kidney neutrophils only heat-stable opsonic activity in normal serum. specific antibody did not show opsonic activity nor any synergism with the normal serum opsonins for either neutrophil population. evidence was found for the production, by plaice neutrophils ...19873829631
[effect of titanium phosphate, zirconium oxide and a mixture of ammonium phosphotungstenate with zirconium phosphate on the elimination of radioactive cesium from the bodies of rats]. 19853835674
isolation and characterization of neuropeptide y from porcine intestine.the isolation and primary structure of intestinal neuropeptide y (npy) is described. the peptide was purified from porcine intestinal extracts using a chemical assay and radioimmunoassay for npy. the amino acid sequence of this peptide is: tyr-pro-ser-lys-pro-asp-asn-pro-gly-glu-asp-ala-pro-ala-glu-asp-leu-ala- arg-tyr-tyr- ser-ala-leu-arg-his-tyr-ile-asn-leu-ile-thr-arg-gln-arg-tyr-nh2. this the structure of intestinal npy is identical to the npy of brain origin.19853838090
isolation and characterization of proteoglycans synthesized by adult human gingival fibroblasts in vitro.the proteoglycans synthesized by fibroblasts derived from healthy human gingivae were isolated and characterized. the largest medium proteoglycan was excluded from sepharose cl-4b but not from sepharose cl-2b; it was recovered in the most-dense density gradient fraction and identified as a chondroitin sulfate proteoglycan. the medium contained two smaller proteoglycans; one contained predominantly chondroitin sulfate proteoglycan, while the other was comprised predominantly of dermatan sulfate p ...19873566283
a signal sequence domain essential for processing, but not import, of mitochondrial pre-ornithine carbamyl transferase.studies using deletion mutagenesis indicate that a processing recognition site lies proximal to the normal cleavage position between gln32 and ser33 of pre-ornithine carbamyl transferase (poct). poct cdna was manipulated to delete codons specifying amino acids 22-30 of the signal sequence. the mutant precursor, designated poct delta 22-30, was imported to the matrix compartment by purified mitochondria, but remained largely unprocessed; the low level of processing that was observed did not invol ...19873571328
neuronal inhibition of astroglial cell proliferation is membrane mediated.previously we have used a microwell tissue culture assay to show that early postnatal mouse cerebellar astroglia have a flattened morphology and proliferate rapidly when they are cultured in the absence of neurons, but develop specific cell-cell contacts and undergo morphological differentiation when they are co-cultured with purified granule neurons (hatten, m. e., 1985, j. cell biol., 100:384-396). in these studies of cell binding between neurons and astroglia, measurement with light and fluor ...19873571332
na+ modulates the k+ permeability and the membrane potential of alkalophilic bacillus.in the absence of na+ in the medium, the membrane potential of obligately alkalophilic bacillus cells was found to be decreased by the addition of k+ to the medium, whereas k+ addition in the presence of na+ had no effect. rb+ showed essentially the same effect as k+. the decreased membrane potential was quickly restored by lowering the k+ concentration in the medium or by adding na+ or li+ to the medium. thus, in the absence of na+, the membrane potential of alkalophilic bacillus seems to be af ...19873663674
liposome as an adjuvant for the production of estradiol antibodies.mice immunized with liposome associated estradiol-bovine serum albumin conjugate (e2-bsa) showed a significantly higher estradiol-specific immune response than did mice injected with the free conjugate. the conjugation of estradiol with bsa is imperative, since injection of liposomes with estradiol in the lipid bilayer was found to be ineffective in inducing an immune response. entrapment of e2-bsa in neutral and positively charged liposomes was found to be a potent mode of immunostimulation, ev ...19873666803
growth of bacteria on chitin, fungal cell walls and fungal biomass, and the effect of extracellular enzymes produced by these cultures on the antifungal activity of amphotericin b.vibrio alginolyticus, streptomyces griseus, arthrobacter g12, bacillus sp. and cytophaga sp. were grown on solid and liquid media containing soluble and insoluble carbon sources. arthrobacter g12, bacillus sp. and cytophaga sp. grew well on media which contained fungal cell walls or fungal biomass as the main carbon source. all bacteria produced extracellular proteases and all bacteria except arthrobacter g12 produced extracellular chitinases. growth of cytophaga sp. on colloidal chitin was para ...19853929028
critical concentration of cadmium for renal toxicity in rats.to evaluate a critical concentration concept of cadmium (cd) toxicity on the kidney, relationships of renal cd level with urinary excretion of various substances--i.e., metallothionein, alkaline phosphatase, lactate dehydrogenase, n-acetyl-beta-d-glucosaminidase, total protein, cd, copper, and zinc--were studied in cd-injected rats. at the renal cd concentration of 100-200 micrograms/g tissue, a dramatic increase of all these substances in urine was observed, supporting the idea of the critical ...19873682016
age-dependent activation of glucocorticoid receptors in the cerebral hemispheres of male rats.the binding of [3h]dexamethasone-receptor complexes to purified nuclei was studied in the cerebral hemispheres of immature (3-week-old) and mature (26-week-old) long-evans male rats to determine the age-related changes, if any, in the physicochemical properties of glucocorticoid receptors. our data show that heat activation (for 45 min at 25 degrees c) significantly enhances the nuclear binding of [3h]dexamethasone-receptor complexes in rats of both ages, with a greater magnitude in immature rat ...19873690338
an nadh:quinone oxidoreductase of the halotolerant bacterium ba1 is specifically dependent on sodium ions.the rate of nadh oxidation by inverted membrane vesicles prepared from the halotolerant bacterium ba1 of the dead sea is increased specifically by sodium ions, as observed earlier in whole cells. the site of this sodium effect is identified as the nadh: quinone oxidoreductase, similarly to the other such system known, vibrio alginolyticus (h. tokuda and t. unemoto (1984) j. biol. chem. 259, 7785-7790). sodium accelerates quinone reduction severalfold, but oxidation of the quinol, with oxygen as ...19863947089
renal apical membrane cholesterol and fluidity in regulation of phosphate transport.renal proximal tubule cells adapt to dietary phosphate (pi) restriction by increasing pi transport independent of parathyroid hormone, vitamin d metabolites, or serum ca2+. to determine the underlying cellular mechanism(s), brush border (bbm) and basolateral membranes (blm) were isolated from growing male rats fed a synthetic diet containing variable levels of pi (0.1-1.4%). dietary pi restriction was without effect on either bbm or blm total lipid phosphorus, individual phospholipid species, or ...19853160247
[etiological role of genital mycoplasma in non-gonococcal urethritis and complications]. 19863963999
evidence for the presence of a novel biosynthetic pathway for norspermidine in vibrio.enzymatic studies of the cell extracts of vibrio alginolyticus and v. parahaemolyticus provided evidence that there exists a novel biosynthetic pathway for norspermidine (nh2(ch2)3nh(ch2)3nh2), a major polyamine species. in this pathway, the schiff base formed between aspartic beta-semialdehyde and 1,3-diaminopropane is first reduced by a nadph-dependent enzyme to yield "carboxynorspermidine" (nh2(ch2)3nh(ch2)2ch(nh2)cooh), which is in turn decarboxylated by a pyridoxal phosphate dependent enzym ...19863697846
lesioning of the rat basal forebrain leads to memory impairments in passive and active avoidance tasks.effects of the bilateral electrolytic lesioning of the basal forebrain (bf), including the ventral globus pallidus, on passive or active avoidance tasks, were studied in male wistar rats. a severe deficit in acquisition of passive avoidance response was produced by the lesioning in the posterior level of bf. the retention of the passive avoidance response was markedly disrupted with post-training lesioning. time-dependent but only slight recovery from the memory impairments was observed in the p ...19853971182
vibrio alginolyticus conjunctivitis. first reported case.a gravely ill patient had vibrio alginolyticus conjunctivitis develop, possibly from contact with seashell fragments. it is believed to be the first reported instance of ocular infection by this organism. marine vibrio organisms must be considered as potential sources of ocular infection in patients with direct or indirect exposure to salt water habitats, or in patients who are immunocompromised.19853977694
[man in prolonged space flight]. 19873307203
energy cost and metabolic regulation during intermittent and continuous tetanic contractions in human skeletal muscle.muscle atp turnover, glycogenolytic, and glycolytic rates were estimated to compare the energy cost and glycolytic regulation of 102.4 s of continuous and intermittent stimulation. quadriceps femoris muscles of male subjects were stimulated at 20 hz for one continuous contraction (n = 6) or a series of 64 contractions (1.6 s on, 1.6 s off; n = 6). leg blood flow was occluded and muscle biopsies were obtained at rest and following 51.2 and 102.4 s of contraction time in both conditions. isometric ...19883370544
sucrose uptake is driven by the na+ electrochemical potential in the marine bacterium vibrio alginolyticus.na+ was found to be essential for the accumulation of sucrose by vibrio alginolyticus. sucrose uptake was completely inhibited by the addition of proton conductor at neutral ph, but not at alkaline ph, where the primary electrogenic na+ pump generates the na+ electrochemical gradient. we therefore conclude that sucrose transport is driven by the electrochemical potential of na+ in this organism.19854030698
vibrio alginolyticus conjunctivitis. 19854038119
comparison of gastrointestinal ph in dogs and humans: implications on the use of the beagle dog as a model for oral absorption in humans.gastrointestinal ph as a function of time was recorded for 4 beagle dogs and 10 human subjects using radiotelemetric ph measuring equipment. results indicated that in the quiescent phase, gastric ph in the dogs (mean = 1.8 +/- 0.07 sem) was significantly (p less than 0.05) higher than in humans (1.1 +/- 0.15). no significant difference in the time for the ph monitoring device to empty from the stomach was noted for the two species (99.8 +/- 27.2 min for dogs, 59.7 +/- 14.8 min for humans, p grea ...19863701609
risk assessment for acquiring meningitis tuberculosis among children not vaccinated with bcg: a case-control study.this case-control study was conducted to assess the risk, among children aged 0-12 years, of developing meningitis tuberculosis (mt) associated with a lack of intradermal bcg vaccination. cases (45) of mt admitted for treatment at the fundacao benjamin guimaraes hospital (belo horizonte, mg, brazil), from 1975 to 1981, were matched for age at hospitalization, date of hospitalization and nutritional status, with two types of controls--patients with acute diarrhoea (ad) and patients with acute non ...19883384537
a method for repeated csf sampling in the freely moving rat.with the use of a holder mounted on the skull of rats, a cannula can be implanted so that one end rests on the atlanto-occipital membrane overlying the cisterna magna. needles introduced through the cannula allow repeated access to the cisterna magna in the freely moving animal. this system is stable for periods of up to 4 weeks and is suited for repeated sampling of the cerebrospinal fluid.19863747592
comparison of api 20e, api rapid e, and api rapid nft for identification of members of the family vibrionaceae.sixty isolates, from nine species of the family vibrionaceae, were tested by the api 20e, api rapid e, and api rapid nft systems. results were compared with those obtained with standard biochemicals. included were 7 aeromonas caviae isolates, 27 aeromonas hydrophilia isolates, 10 aeromonas sobria isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolat ...19854056003
an infection by vibrio alginolyticus in an atlantic bottlenose dolphin housed in an open ocean pen. 19854078981
morphological changes in rat striatal boutons after chronic methamphetamine and haloperidol treatment.dopaminergic (da) synaptic boutons were identified in rat striatum with an electron microscopic histochemical method. in rats which developed behavioral hypersensitivity after treatment with methamphetamine (map) for about 2 weeks, significantly fewer da boutons were found. this effect was specific to da boutons without mitochondria and was not seen in boutons with mitochondria. the density of granular synaptic vesicles in da boutons, however, did not change significantly. these morphological ch ...19863748472
characterization and distribution of vibrio alginolyticus and vibrio parahaemolyticus isolated in indonesia.previous studies have shown that vibrio alginolyticus and vibrio parahaemolyticus can be isolated from similar types of marine samples. in this report, the results of an examination of 567 v. alginolyticus and v. parahaemolyticus strains, isolated from seawater in jakarta bay and from more than 30 types of seafood from markets in jakarta, indonesia, are presented. most isolates were from mackerel, shrimp, or squid. numerical taxonomic analyses clustered 337 isolates and three v. alginolyticus re ...19854091566
the action of cyanogen bromide on rabbit igg molecules of allotypes a11 and a12. 19704097709
the effects of lindane on laying hens. 19724112976
giant flagellar bundles of vibrio alginolyticus (ncmb 1803). 19734131532
histological evidence supporting the inferior olive as the major source of cerebellar climbing fibers in the rat. 19744136782
electrophoretic separation of x- and y-chromosome-bearing sperm in human semen. 19744141808
half-life of diphenylpyraline in man. 19744156058
do viruses cause cancer in man? 19684171855
structures of the lymph node and their possible function during the immune response. 19684178481
proposal of vibrio alginolyticus for the biotype 2 of vibrio parahaemolyticus. 19684180095
influence of ototoxic drugs on acetylcholine-induced depression of the cochlear n1 potential. 19704195594
an enzymic-chemical degradation of glucose. 19684236515
[effects of sulpiride on the genital system of the male golden hamster in the winter involution phase]. 19714261921
nicotinic acid loading of normal cows: effects on blood metabolites and excretory forms. 19724263241
failure of orally administered perchlorate to affect deciduoma formation or pregnancy in the rat. 19664288675
failure of orally administered perchlorate to affect deciduoma formation or pregnancy in the rat. 19664288675
the experimental production of ischemic facial paralysis. 19664288689
the experimental production of ischemic facial paralysis. 19664288689
studies on hepatic glycolytic enzymes of normal and scorbutic guinea pigs administered acetoacetate. 19684299211
studies on hepatic glycolytic enzymes of normal and scorbutic guinea pigs administered acetoacetate. 19684299211
[the study of the formation of lecithinase by clostridium perfringens type a]. 19684300426
the effect of the seed of musa superba roxb. on small-pox virus. 19674302598
chloride ion as a modifier of 2',3'-cyclic phosphodiesterase purified from halophilic vibrio alginolyticus. 19694303200
relationship between the active sites of 2',3'-cyclic phosphodiesterase with 3'-nucleotidase activity purified from vibrio alginolyticus. 19694307679
effects of chloroquine pretreatment on lethal dose of morphine and on morphine metabolism in rats. 19694309711
effects of chloroquine pretreatment on lethal dose of morphine and on morphine metabolism in rats. 19694309711
role of hypothalamic monoamines in the control of pituitary secretions. 19694319515
anion-activated 5'-nucleotidase in cell envelopes of a slightly halophilic vibrio alginolyticus. 19704321167
[ultrastructure of the proctodeum of the thysanuran lepismodes inquilinus newman (=therobia domestica packard). i. anterior region (ileum and rectum)]. 19714329667
effects of antibiotics on the development and stability of mitochondrial enzymes in saccharomyces cerevisiae. 19724343088
effect of free magnesium and salts on the inorganic pyrophosphatase purified from a slightly halophilic vibrio alginolyticus. 19734360431
aldosterone: current concepts. 19744363421
the chemical and enzymatic oxidation of hematohemin ix. identification of hematobiliverdin ix alpha as the sole product of the enzymatic oxidation.oxidative cleavage of hematohemin ix in pyridine solution in the presence of ascorbic acid (coupled oxidation), followed by esterification of the products with boron trifluoride/methanol produced the four possible hematobiliverdin dimethyl esters in 11.1% overall yield. transetherifications took place simultaneously with the esterification reaction and resulted in the formation of the dimethyl ester of hematobiliverdin ix gamma 8a,13a-dimethyl ether (1.8%), the dimethyl ester of hematobiliverdin ...19863754208
duchenne muscular dystrophy in a girl with an (x;15) translocation.this is a report of a girl with duchenne muscular dystrophy (dmd) associated with an 46,x,t (x;15) (p21; q 26) chromosome constitution. although in the eight published cases of girls with dmd and a t(x;aut) different autosomes were involved in the translocation, the breakpoint was always at xp21. the present case supports the hypothesis that the dmd gene must be located at xp21. in this study, involvement of the father's chromosomes in the translocation was detected.19863777020
national surveillance of aids in health care workers.information obtained for all persons with the acquired immunodeficiency syndrome (aids) reported to the centers for disease control, atlanta, includes a question about employment in a health care or clinical laboratory setting. as of may 1, 1986, a total of 922 (5.5%) of 16,748 adults with aids reported employment in such settings. ninety-five percent of these health care workers belonged to recognized high-risk groups for aids; the proportion with "no identified risk" has not increased with tim ...19863783866
studies on transferrin receptor expression in mouse plasmacytoma cells. 19863792031
analysis of 5-ht1 binding site subtypes and potential functional correlates. 19863797586
trimethoprim used for selective decontamination of the digestive tract in rats: possible route of excretion.selective elimination of enterobacteriaceae species from the digestive tract of rats has been accomplished by oral treatment with trimethoprim (235 mg/kg body weight/day) within 6 days. in the present study it was investigated whether this elimination was mainly due to antimicrobial activity of trimethoprim excreted with the gastrointestinal mucus or mainly by non-absorbed trimethoprim in the lumen contents. by means of whole gut irrigation (wgi) the lumen contents were washed out, followed by m ...19873497436
leishmania in the old world: 3. the distribution of l. aethiopica zymodemes.isoenzyme profiles of 28 stocks of leishmania aethiopica were compared with those of reference strains of l. aethiopica, l. tropica and l. major using starch-gel electrophoresis of 13 enzymes (gpi, gd, es, pgm, pepd, nh, asat, alat, pk, mpi, 6pgd, sod, mdh). 13 zymodemes were seen. l. aethiopica showed some infraspecific variation. stocks from phlebotomus longipes and procavia habessinica were indistinguishable from those from man. stocks from cases of diffuse cutaneous and cutaneous leishmanias ...19863798530
anticandidal activities of terconazole, a broad-spectrum antimycotic.terconazole is a new triazole ketal derivative with broad-spectrum in vitro and in vivo antifungal activities. this study further characterizes the effects of terconazole in vitro on yeast cell growth, viability, and morphology. terconazole inhibited the growth of candida albicans atcc 44859 in a concentration-related manner, but with modest effects noted at levels from 10(-8) to 10(-5) m when the yeast was grown on media favoring the cell form. the inhibitory potency of terconazole on yeast cel ...19863729366
processing-independent in vitro translocation of cytochrome p-450(scc) precursor across mitochondrial membranes.in the presence of a membrane-permeable metal chelator, bovine adrenal cortex mitochondria imported p-450(scc) precursor without processing of the amino-terminal extension peptide. the imported precursor was bound to the matrix side surface of the inner membrane. when the inhibition due to the metal chelator was removed, the imported precursor was processed to the mature form. unprocessed precursor was also detected in mitochondria when the import reaction was carried out at relatively low tempe ...19863818579
is raised plasma peptide yy after intestinal resection in the rat responsible for the trophic response?the relationship between the adaptive response and plasma pyy concentrations after small bowel resection has been investigated. seventy five per cent proximal small bowel resection resulted in a rise in plasma pyy at six days from 28 +/- 3.1 to 85 +/- 12.3 pmol/l (p less than 0.001) and this difference was maintained to 48 days. plasma pyy correlates both with crypt cell production rate (ccpr) in the ileum and with plasma enteroglucagon levels. in a second study, pyy or saline was infused over a ...19853841330
sequence analysis of peptide fragments from the intrinsic membrane protein of calf lens fibers mp26 and its natural maturation product mp22.calf lens fiber plasma membranes, containing only the intrinsic membrane protein mp26 and its maturation product mp22 were treated with proteolytic enzymes such as trypsin, protease v8 from s. aureus or with chemical agents as cnbr in formic acid. the cleavage products, purified by electrophoresis, were analysed for their amino acid composition and n-terminal sequences. proteolysis gave rise to peptides which were mainly shortened at the c-terminal end of the molecules. while the v8 protease pro ...19853882455
cardioprotective effects of defibrotide in acute myocardial ischemia in the cat.we investigated the effects of defibrotide (d), a natural polydeoxyribonucleotide, on acute myocardial ischemia (ami) in anesthetized cats. a permanent ligature was placed around the left anterior descending coronary artery (lad) 12-14 mm from its origin. ventricular fibrillation and death were exceptional and when they occurred the cats were not included in the evaluation. pretreatment of cats with d, 32 mg kg-1 h-1, i.v. infusion, maintained throughout the 5 h occlusion period, reduced ami-st ...19853890260
cardioprotective effects of defibrotide in acute myocardial ischemia in the cat.we investigated the effects of defibrotide (d), a natural polydeoxyribonucleotide, on acute myocardial ischemia (ami) in anesthetized cats. a permanent ligature was placed around the left anterior descending coronary artery (lad) 12-14 mm from its origin. ventricular fibrillation and death were exceptional and when they occurred the cats were not included in the evaluation. pretreatment of cats with d, 32 mg kg-1 h-1, i.v. infusion, maintained throughout the 5 h occlusion period, reduced ami-st ...19853890260
[root submergence: prevention of jaw resorption. review of the literature]. 19853909247
effect of melanin concentrating hormone on pigment and adrenal cells in vitro.highly purified synthetic salmonid melanin concentrating hormone (mch) and some analogs were investigated for their ability to concentrate the pigment in scale melanophores of the chinese grass carp, ctenopharyngodon idellus, to produce melanin dispersion in frog or lizard melanophores and to inhibit alpha-msh in its action on mouse melanoma and rat adrenal glomerulosa cells in vitro. in the grass carp, mch produced half-maximal pigment aggregation at 6 x 10(-11) m and its oxidized form at 7 x 1 ...19853938841
phospholipase a2-treated human high-density lipoprotein and cholesterol movements: exchange processes and lecithin: cholesterol acyltransferase reactivity.human hdl3 (d 1.125-1.21 g/ml) were treated by an exogenous phospholipase a2 from crotalus adamenteus in the presence of albumin. phosphatidylcholine hydrolysis ranged between 30 and 90% and the reisolated particle was essentially devoid of lipolysis products. (1) an exchange of free cholesterol was recorded between radiolabelled erythrocytes at 5-10% haematocrit and hdl3 (0.6 mm total cholesterol) from 0 to 12-15 h. isotopic equilibration was reached. kinetic analysis of the data indicated a co ...19863942760
specialized contacts between sarcolemma and sarcoplasmic reticulum at the ends of muscle fibers in the diaphragm of the rat.an electron-microscopic study of the myotendinous portion of the diaphragm in the wistar rat has shown that at the ends of muscle fibers, longitudinally oriented invaginations and peripheral furrows of the sarcolemma establish specialized contacts with individual sacs of the sarcoplasmic reticulum. the construction of these terminal contacts is similar to that of contacts between sarcolemmic t-tubules and terminal cisternae of the sarcoplasmic reticulum, characterized by formation of triads. the ...19863948240
identical chromosome translocations involving the region of the c-myb oncogene in four metastases of a mediastinal teratocarcinoma.a 39-year-old white male presented with a disseminated mediastinal teratocarcinoma. karyotyping was performed on two mature residual metastatic lesions in the lungs immediately following chemotherapy, on a recurring lung lesion after 5 months, and on a metastasis in the right thigh 5 months after salvage chemotherapy. all four lesions were pseudoeuploid and showed identical chromosomal abnormalities: a translocation with the two chromosomes #6 and one chromosome #11 involved, resulting in 46, xy ...19853967222
lateral flagellar antigen of vibrio alginolyticus and vibrio harveyi: existence of serovars common to the two species.the antigenicity of lateral (l-) flagella of two marine vibrios, vibrio alginolyticus and v. harveyi, was studied, and the two species were found to have common antigenicity of their flagella. antisera against l-flagella were prepared by immunizing rabbits with highly purified l-flagellar filaments. h-agglutination tests with the anti-l-flagella antisera showed that four h-serovars existed in these species and that two of them were shared by the two species. cross reactivity between h-serovars o ...19854010543
effect of stilboestrol on adrenal steroidogenesis in albino rats. 19854018847
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