effect of enzymatic debridement with two different collagenases versus mechanical debridement on chronic hard-to-heal wounds.a chronic ulcer is usually defined as an injury that does not spontaneously evolve towards healing and does not progress through normal healing stages such as inflammation, proliferation and remodelling. this study was designed in order to compare two types of collagenases with mechanical debridement alone. it was thus possible to evaluate their differences in terms of pain and debridement efficacy. patients were divided into three groups: 30 patients were daily dressed using an ointment based o ...201625649929
immunological evaluation of vibrio alginolyticus, vibrio harveyi, vibrio vulnificus and infectious spleen and kidney necrosis virus (isknv) combined-vaccine efficacy in epinephelus coioides.combined vaccines are immunological products intended for immunization against multifactorial infectious diseases caused by different types or variants of pathogens. in this study, the effectiveness of vibrio alginolyticus (va), vibrio harveyi (vh), vibrio vulnificus (vv) and infectious spleen and kidney necrosis virus (isknv), an iridovirus, combined-vaccine (vibrio and isknv combined vaccines, vicv), va+vh+vv inactive vaccine (viv) and isknv whole cell inactive vaccine (iwciv) in epinephelus c ...201223010220
effects of hot-water extract of banana (musa acuminata) fruit's peel on the antibacterial activity, and anti-hypothermal stress, immune responses and disease resistance of the giant freshwater prawn, macrobrachium rosenbegii.the hot-extracts isolated from fruit's peel of banana, musa acuminata, was evaluated on the antibacterial activity to pathogens from aquatic animals, and immunostimulating potential, disease resistance and anti-hypothermal stress in giant freshwater prawn, macrobrachium rosenbergii through injection administration. the banana peel extract (bpe) showed good activity against 1 gram-positive and 3 gram-negative pathogens, including lactococcus garvieae, photobacteria damsella, vibrio alginolyticus ...201424906123
the first mollusk spätzle homolog gene in the clam, paphia undulate.spätzle, is the only identified endogenous toll receptor ligand, plays a critical role in initiatinge innate immune responses and controlling dorsal-ventral axis formation in drosophila. here we identified the first spätzle gene homolog, pu-spz, in the marine mollusk paphia undulate. the full-length of pu-spz cdna is 1248 bp, including an open reading frame (orf) of 702 bp, a 5'-untranslated region (utr) of 26 bp and a 3'-utr of 203 bp. the orf encodes a 233-amino-acid protein with conserved dom ...201526477575
expression and function analysis of two naturally truncated myd88 variants in the pacific oyster crassostrea gigas.myeloid differentiation factor 88 (myd88) is the classic signaling adaptor that mediates toll/interleukin-1 receptor (tir/il-1r) dependent activation of nuclear factor-kappa b (nf-κb). in this study, two naturally truncated myd88 members were identified from the pacific oyster (crassostrea gigas), namely cgmyd88-t1 and cgmyd88-t2. the full-length cdna of cgmyd88-t1, cgmyd88-t2 are 976 bp and 1038 bp in length, containing an orf of 552 bp and 555 bp, respectively. the two orf encode a putative pr ...201525963623
the first invertebrate rig-i-like receptor (rlr) homolog gene in the pacific oyster crassostrea gigas.retinoic acid-inducible gene i (rig-i)-like receptor (rlr) is a pivotal receptor that detects numerous rna and dna viruses and mediates the innate induction of interferons and pro-inflammatory cytokines upon viral infection. in the present study, we cloned and characterized the first rig-i gene in a marine mollusk, crassostrea gigas, and designated it as cgrig-i. the full-length cgrig-i cdna is 3436 bp, including 5'- and 3'-untranslated regions (utrs) of 93 bp and 286 bp, respectively, and an op ...201425107697
genomic characterization and expression analysis of five novel il-17 genes in the pacific oyster, crassostrea gigas.interleukin-17 (il-17) is a proinflammatory cytokine that plays an important role in clearing extracellular bacteria and contributes to the pathology of many autoimmune and allergic conditions. in the present study, five novel il-17 homologs were identified by searching and analyzing the pacific oyster genome. all six cgil-17 members (including a previously reported homolog) contained four conserved cysteines that were used in the formation of disulfide bonds. phylogenetic analysis showed that a ...201425090939
identification and function analysis of an anti-lipopolysaccharide factor from the ridgetail prawn exopalaemon carinicauda.anti-lipopolysaccharide factor is a kind of antimicrobial peptide (amp) with broad-spectrum activities against bacteria, virus, and fungi in crustacean. different isoforms of alfs showed different activities to virus or bacteria. therefore, discovery of more alfs will provide new insights into drug development and disease control. here we reported an alf gene, ecalf1, isolated from the ridgetail prawn exopalaemon carinicauda. its transcripts was mainly detected in hemocytes. silencing of ecalf1 ...201728082184
molecular cloning and characterization of a new g-type lysozyme gene (ec-lysg) in orange-spotted grouper, epinephelus coioides.lysozyme acts as an innate immunity molecule against pathogen infection. in this study, a new g-type lysozyme gene with a typical g-type lysozyme domain (designated as ec-lysg) was cloned and characterized from the orange-spotted grouper, epinephelus coioides. the full-length ec-lysg cdna contains 1419 bp and encodes a 256-residue protein containing a 25-residue signal peptide at the n-terminus. blast analysis reveals ec-lysg shares 64% identity with siniperca chuatsi, but 63% to another reporte ...201424877656
transcriptome profiling analysis on whole bodies of microbial challenged eriocheir sinensis larvae for immune gene identification and snp study crab immunogenetics of individuals, newly hatched eriocheir sinensis larvae were stimulated with a mixture of three pathogen strains (gram-positive bacteria micrococcus luteus, gram-negative bacteria vibrio alginolyticus and fungi pichia pastoris; 10(8) cfu·ml(-1)). a total of 44,767,566 illumina clean reads corresponding to 4.52 gb nucleotides were generated and assembled into 100,252 unigenes (average length: 1,042 bp; range: 201-19,357 bp). 17,097 (26.09%) of 65,535 non-redundant uni ...201324324760
crustins from eyestalk cdna library of swimming crab portunus trituberculatus: molecular characterization, genomic organization and expression analysis.crustins are cationic, cysteine-rich antimicrobial proteins, containing a single whey acidic protein (wap) domain in the c-terminal end. different from the reported ptcrustin in the hemocytes, two novel crustin genes (ptcrustin2 and ptcrustin3) were cloned completely from the eyestalk cdna library of portunus trituberculatus in this study. all ptcrustins share the consensus cysteine motif and are considered as type i crustins. four exons and three introns are identified in genomic dna sequence o ...201222971359
molecular cloning and characterization of c-type lysozyme gene in orange-spotted grouper, epinephelus coioides.lysozymes are key proteins of the host innate immune system against pathogen infection. in this study, a c-type lysozyme gene (ec-lysc) was cloned and characterized from orange-spotted grouper, epinephelus coioides. the full-length ec-lysc cdna is composed of 533 bp and encodes a polypeptide of 144-residue protein with 94% identity to lysc of kelp grouper, epinephelus bruneus. the genomic dna of ec-lysc consists of 4 exons and 3 introns, with a total length of 1897 bp. amino acid sequence alignm ...201222579550
first report of two thioredoxin homologues in crustaceans: molecular characterization, genomic organization and expression pattern in swimming crab portunus trituberculatus.previously, we had reported two homologues of the thioredoxin (trx) super-family (pttrx1 and pttrx2) identified from eyestalk and haemocytes cdna library of swimming crab portunus trituberculatus, respectively. it was the first report of two thioredoxin homologues from the same crustacean species. here, we focused on the molecular characterization, genomic organization and expression pattern of pttrx1 and pttrx2. the full-length cdna sequences of pttrx1 and pttrx2 were 739 and 1300 bp, encoding ...201222365991
identification and characterization of two novel types of non-clip domain serine proteases (ptsp and ptsph1) from cdna haemocytes library of swimming crab portunus our previous studies, five serine proteases containing clip domain were characterized from the swimming crab portunus trituberculatus. to further investigate the characterization and function of serine proteases, one serine protease (ptsp) and one serine protease homolog (ptsph1) without clip domain were identified from haemocytes cdna library in this paper. they both possessed an sp or sp-like domain at the c-terminal. in contrast to ptsp, absence of ser catalytic residue resulted in the los ...201222289714
three clip domain serine proteases (csps) and one clip domain serine protease homologue (csph) identified from haemocytes and eyestalk cdna libraries of swimming crab portunus trituberculatus.four genes including three clip domain serine proteases (ptcsp1, ptcsp2 and ptcsp3) and one clip domain serine protease homologue (ptcsph) of the swimming crab portunus trituberculatus (decapoda: brachyura: portunidae) were characterized based on analysis of expressed sequence tags from haemocytes and eyestalk cdna libraries. the relative four peptidases, which share high structural similarity to the clip-sps of other arthropod species, appeared to possess a clip domain at the n-terminus and an ...201222270256
development and evaluation of a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip lamp) for rapid and simultaneous detection of ten pathogenic bacteria in aquatic animals.rapid, low-cost, and user-friendly strategies are urgently needed for early disease diagnosis and timely treatment, particularly for on-site screening of pathogens in aquaculture. in this study, we successfully developed a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip lamp), which was capable of simultaneously detecting 10 pathogenic bacteria in aquatic animals, i.e., nocardia seriolae, pseudomonas putida, streptococcus iniae, ...201424954661
tank-binding kinase-1 broadly affects oyster immune response to bacteria and a benthic filter feeder of estuaries, the immune system of oysters provides one of the best models for studying the genetic and molecular basis of the innate immune pathway in marine invertebrates and examining the influence of environmental factors on the immune system. here, the molecular function of molluscan tank-binding kinase-1 (tbk1) (which we named cgtbk1) was studied in the pacific oyster, crassostrea gigas. compared with known tbk1 proteins in other model organisms, cgtbk1 contains ...201627422757
molecular characterization and functional analysis of tumor necrosis factor receptor-associated factor 2 in the pacific oyster.tumor necrosis factor receptor (tnfr)-associated factors (trafs) are a family of crucial adaptors, playing vital roles in mediating signal transduction in immune signaling pathways, including rig-i-like receptor (rlr) signaling pathway. in the present study, a new traf family member (cgtraf2) was identified in the pacific oyster, crassostrea gigas. comparison and phylogenetic analysis revealed that cgtraf2 could be a new member of the invertebrate traf2 family. quantitative real-time pcr reveale ...201626621757
microbiome analysis and detection of pathogenic bacteria of penaeus monodon from jakarta bay and bali.penaeus monodon, the asian black tiger shrimp is one of the most widely consumed marine crustaceans worldwide. in this study, we examine and compare the fecal microbiota of p. monodon from highly polluted waters around jakarta bay, with those of less polluted waters of bali. using next generation sequencing techniques, we identified potential bacterial pathogens and common viral diseases of shrimp. proteobacteria (96.08%) was found to be the most predominant phylum, followed by bacteriodetes (2. ...201627090886
exse is a negative regulator for t3ss gene expression in vibrio alginolyticus.type iii secretion systems (t3sss) contribute to microbial pathogenesis of vibrio species, but the regulatory mechanisms are complex. we determined if the classic exsacde protein-protein regulatory model from pseudomonas aeruginosa applies to vibrio alginolyticus. deletion mutants in v. alginolyticus demonstrated that, as expected, the t3ss is positively regulated by exsa and exsc and negatively regulated by exsd and exse. interestingly, deletion of exse enhanced the ability of v. alginolyticus ...201627999769
molecular cloning, expression pattern and antimicrobial activity of a new isoform of anti-lipopolysaccharide factor from the swimming crab portunus trituberculatus.a new isoform of anti-lipopolysaccharide factor (ptalf5) was cloned from eyestalk cdna library of swimming crab portunus trituberculatus. the full-length cdna of ptalf5 was 1045 bp encoding 120 amino acids. ptalf5 shared lower amino acid similarity with other alfs, yet it contained the conserved lps-binding domain and was clearly member of the alf family. the genomic fragment of ptalf5 contained two exons separated by one intron. several tandem repeats were found in intron. the mrna transcript o ...201222516300
eutrophication, ammonia intoxication, and infectious diseases: interdisciplinary factors of mass mortalities in cultured nile tilapia.the present study was designed to assess the possible causes of the mass mortalities of nile tilapia oreochromis niloticus at el-behera governorate, egypt, in relationship to environmental and microbiotic factors. water samples were collected from fish farms at different locations and from lake edku to analyze water temperature, water ph, salinity, biological oxygen demand, dissolved oxygen, total ammonia nitrogen, and un-ionized ammonia. a number of moribund and freshly dead fish were sampled a ...201627484819
establishment and characterization of a cell line from the head kidney of golden pompano trachinotus ovatus and its application in toxicology and virus susceptibility.a cell line derived from the head kidney of golden pompano trachinotus ovatus (tohk) was established and characterized in this study. the tohk cells grew most rapidly at 28° c and the optimum foetal bovine serum concentration in l-15 medium was 10%. the tohk cells have a diploid chromosome number of 2n = 54. the transfection efficiency of tohk cells was 7·5% at the 15th passage and 72% at the 40th passage. the transfection efficiency in tohk cells was high, so these cells are suitable for foreig ...201728271507
establishment and characterization of a mid-kidney cell line derived from golden pompano trachinotus ovatus, a new cell model for virus pathogenesis and toxicology studies.golden pompano trachinotus ovatus, a popularly cultured and commercially important marine fish worldwide, has been recognized as a promising candidate for mariculture. however, outbreaks of infectious bacterial or viral diseases and environmental deterioration have led to great economic losses in t. ovatus aquaculture recently. in our research, we established a new mid-kidney cell line, designated as tok, from golden pompano, t. ovatus. the optimized growth temperature and working concentration ...201627981423
establishment and characterization of a novel cell line from the brain of golden pompano (trachinotus ovatus).golden pompano is a commercially important marine fish that is widely cultured in china, japan, and southeast asian countries but has been seriously threatened by pathogen. a novel cell line (togb) derived from the brain of golden pompano trachinotus ovatus was established and characterized in this study. togb cell line showed high virus susceptibility, especially grouper nervous necrosis virus (gnnv) and singapore grouper iridovirus (sgiv). as one of the most devastating viruses in marine fish ...201626822430
isolation and characterization of tumor necrosis factor receptor-associated factor 6 (traf6) from grouper, epinephelus tauvina.tumor necrosis factor receptor-associated factor 6 (traf6) is one of the key adapter molecules in toll-like receptor signal transduction that triggers downstream cascades involved in innate immunity. in the present study, a traf6 (named as et-traf6) was identified from the marine fish grouper, epinephelus tauvina by race pcr. the full-length cdna of et-traf6 comprised 1949 bp with a 1713 bp open reading frame (orf) that encodes a putative protein of 570 amino acids. similar to most traf6s, et-tr ...201424811008
establishment of a new cell line from the heart of giant grouper, epinephelus lanceolatus (bloch), and its application in toxicology and virus susceptibility.a new marine fish cell line, derived from the heart of giant grouper, epinephelus lanceolatus (bloch), was established and characterized. the cell line was designated as elgh and subcultured with more than 60 passages. the elgh cells were mainly composed of fibroblast-like cells and multiplied well in leibovitz's l-15 medium supplemented with 10% foetal bovine serum (fbs) at 28 °c. chromosome analysis indicated that the modal chromosome number was 48. the fluorescent signals were detected in elg ...201524372271
molecular characterization of pacific oyster (crassostrea gigas) irak4 gene and its role in myd88-dependent pathway.interleukin-1 receptor-associated kinases (iraks) play important roles in myd88-dependent tlr signaling, the crucial innate immune pathway in molluscs. in this study, we examined the full-length irak4 genetic sequence in the pacific oyster (crassostrea gigas) by molecular cloning. phylogenetic analysis revealed that cgirak4 is most closely related to mytilus edulis, and forms a clade with other molluscs. cgirak4 transcripts are widely expressed in all tissues, with the highest expression observe ...201728223161
c-jun n-terminal kinase (jnk) is involved in immune defense against bacterial infection in crassostrea hongkongensis.c-jun n-terminal kinase (jnk) is a universal and essential subgroup of the mitogen-activated protein kinase (mapk) superfamily, which is highly conserved from yeast to mammals and functions in a variety of physiological and pathological processes. in this study, we report the first oyster jnk gene homolog (chjnk) and its biological functions in the hong kong oyster crassostrea hongkongensis. the chjnk protein consists of 383 amino acids and contains a conserved serine/threonine protein kinase (s ...201727840294
optimization and characterization of biosurfactant production from marine vibrio sp. strain 3b-2.a biosurfactant-producing bacterium, designated 3b-2, was isolated from marine sediment and identified as vibrio sp. by 16s rrna gene sequencing. the culture medium composition was optimized to increase the capability of 3b-2 for producing biosurfactant. the produced biosurfactant was characterized in terms of protein concentration, surface tension, and oil-displacement efficiency. the optimal medium for biosurfactant production contained: 0.5% lactose, 1.1% yeast extract, 2% sodium chloride, an ...201526441908
identification and function of an evolutionarily conserved signaling intermediate in toll pathways (ecsit) from crassostrea hongkongensis.evolutionarily conserved signaling intermediate in toll pathways (ecsit) is a multifunctional adaptor protein that plays a key role in the regulation of the oxidative phosphorylation (oxphos) system, bone morphogenetic protein (bmp) pathway and toll-like receptor (tlr) signaling pathway in mammals. however, the function of ecsit homologs in mollusks, the second most diverse group of animals, is not well understood. in this study, we identified an ecsit homolog in the hong kong oyster crassostrea ...201526204814
a novel molluscan fos gene with immune defense function identified in the hong kong oyster, crassostrea hongkongensis.the transcription factor fos is a member of one of the best-studied ap-1 sub-families and has been implicated in a wide variety of biological processes, including the regulation of apoptosis, immune responses and cytokine production. in this report, a novel mollusk fos (referred to as chfos) gene was cloned and characterized from the hong kong oyster, crassostrea hongkongensis. the deduced chfos protein sequence comprised 333 amino acids and shared significant homology with invertebrate homologs ...201525841657
prostaglandin e receptor 4 (ptger4) involved in host protection against immune challenge in oyster, crassostrea hongkongensis.prostaglandin e receptor 4 (ptger4) is an essential receptor that can detect various physiological and pathological stimuli and has been implicated in a wide variety of biological processes, including the regulation of immune responses, cytokine production, and apoptosis. in this report, the first mollusk ptger4, referred to as chptger4, was cloned and characterized from the hong kong oyster crassostrea hongkongensis. its full-length cdna is 1734 bp in length, including 5'- and 3'-untranslated r ...201525463295
the identification of the first molluscan akirin2 with immune defense function in the hong kong oyster crassostrea hongkongensis.the akirin protein is a nuclear factor in the innate immune system that is highly conserved from insects to mammals and plays key roles in diverse biological processes, including immunity, myogenesis, development and the cellular stress response. however, the function of akirins in mollusk, the second most diverse group of animals, is still poorly understood. in this study, we report the discovery of an akirin2 gene homolog (chakirin2) and its biological functions in the hong kong oyster crassos ...201425284180
the first molluscan acute phase serum amyloid a (a-saa) identified from oyster crassostrea hongkongensis: molecular cloning and functional characterization.serum amyloid a (saa), a major evolutionarily conserved acute-phase protein, participates in many biological processes in eukaryotic cells, including innate immunity. however, little information regarding the relationship between saa and innate immunity in mollusks is currently available. in this report, the first bivalve saa (referred to as chsaa) gene was identified and characterized from the hong kong oyster crassostrea hongkongensis. its full-length cdna is 623 bp, including a 5'-utr of 147 ...201424859593
characterization and functional analysis of serine proteinase and serine proteinase homologue from the swimming crab portunus trituberculatus.serine proteases (sps), with their homologues (sphs), a family of multifunctional proteins, play a crucial role in innate immune system. in our present study, we made an appropriate correction: serine protease homologue ptcsph (li et al., [1]) obtained from the swimming crab portunus trituberculatus was actually a serine protease and re-designated as ptcsp. sequence analysis revealed ptcsp and ptsp (li et al., [2]) might be encoded by the same genomic locus and generated by alternative splicing ...201323664866
phenol, 2,4-bis(1,1-dimethylethyl) of marine bacterial origin inhibits quorum sensing mediated biofilm formation in the uropathogen serratia marcescens.intercellular communication in bacteria (quorum sensing, qs) is an important phenomenon in disease dissemination and pathogenesis, which controls biofilm formation also. this study reports the anti-qs and anti-biofilm efficacy of seaweed gracilaria gracilis associated vibrio alginolyticus g16 against serratia marcescens. purification and mass spectrometric analysis revealed the active principle as phenol, 2,4-bis(1,1-dimethylethyl) [pd]. pd affected the qs regulated virulence factor production i ...201425377484
a chimeric siderophore halts swarming vibrio.some bacteria swarm under some circumstances; they move rapidly and collectively over a surface. in an effort to understand the molecular signals controlling swarming, we isolated two bacterial strains from the same red seaweed, vibrio alginolyticus b522, a vigorous swarmer, and shewanella algae b516, which inhibits v. alginolyticus swarming in its vicinity. plate assays combined with nmr, ms, and x-ray diffraction analyses identified a small molecule, which was named avaroferrin, as a potent sw ...201424615751
antibacterial activity of metabolites products of vibrio alginolyticus isolated from sponge haliclona sp. against staphylococcus aureus.the objective of this study was to investigate the antibacterial activity of primary and secondary metabolites from vibrio alginoliticus isolated from sponge haliclona sp. against staphylococcus aureus. a descriptive method was used in this research. the antibacterial activity was analysed by paper disk method. the results showed that the primary metabolites produced by vibrio alginoliticus that is in symbiosis with sponge haliclona sp. were able to effectively inhibit staphylococcus aureus grow ...201728299291
identification, ontogeny and expression analysis of a novel laboratory of genetics and physiology 2 (lgp2) transcript in asian seabass, lates calcarifer.lgp2 (laboratory of genetics and physiology 2) is an important member of the retinoic acid-inducible gene i (rig-i)-like receptors (rlrs), which plays a significant role in antiviral innate immunity. in this study, we have cloned the full-length cdna sequence of lgp2 from asian seabass, lates calcarifer (aslgp2). the complete aslgp2 cdna sequence consisted of 2586 nucleotides encoding a putative protein of 681 amino acids with a molecular mass of 77.6 kda. from the aslgp2 protein, four different ...201728119144
identification and functional characterization of a novel spätzle gene in litopenaeus vannamei.shrimp innate immunity is the first line of resistance against pathogenic bacteria. the toll-like receptor (tlr)-nf-κb pathway is vital in this immunity process. in this study, a novel spätzle gene (lvspz4) of litopenaeus vannamei was cloned and functionally characterized. the open reading frame of lvspz4 was 918 bp, which encoded a putative protein with 305 amino acids. lvspz4 was most expressed in the gills of l. vannamei. this expression was induced by vibrio alginolyticus or staphylococcus a ...201727884706
identification of nod like receptor c3 (nlrc3) in asian seabass, lates calcarifer: characterisation, ontogeny and expression analysis after experimental infection and ligand stimulation.nod like receptors (nlrs) are a large group of cytoplasmic prrs believed to play an important role in bacterial recognition in higher vertebrates. in this study, a novel nod like receptor c3 (asnlrc3) has been identified, cloned and characterised from asian seabass, lates calcarifer. the full-length asnlrc3 transcript composed of a 4142 bp nucleic acid sequence encode for a protein of 1134 deduced amino acids. three signature domains identified are conserved nacht-domain, c-terminal llr domain a ...201627346158
identification and functional characterization of heat shock transcription factor1 in litopenaeus vannamei.heat shock transcription factors belong to the heat shock factor (hsf) protein family, which are involved in heat shock protein (hsp) gene regulation. they are critical for cell survival upon exposure to harmful conditions. in this study, we identified and characterized a hsf1 (lvhsf1) gene in litopenaeus vannamei, with a full-length cdna of 2841 bp and an open reading frame encoding a putative protein of 632 amino acids. through multiple sequence alignment and phylogenetic analysis, it was reve ...201424508618
molecular characterization and function of a p38 mapk gene from litopenaeus vannamei.p38 mitogen-activated protein kinases (mapks) are broadly expressed from yeasts to mammals, and are involved in the regulation of cells responsible to various extracellular stimuli. in this study, a p38 mapk gene (designated as lvp38) from litopenaeus vannamei, was cloned and characterized. it contained the conserved structures of a thr-gly-tyr (tgy) motif and a substrate-binding site, ala-thr-arg-trp (atrw). the tissue distribution patterns showed that lvp38 was widely expressed in all examined ...201323500954
molecular identification and functional characterization of a tumor necrosis factor (tnf) gene in crassostrea hongkongensis.tumor necrosis factor superfamily (tnfsf) represents a group of multifunctional inflammatory cytokines that have been shown to participate in a variety of pathological and immunological process. however, the functions of these proteins in oyster are still poorly understood. in the present study, an oyster tnf homolog (named chtnf) was identified from a cdna library of crassostrea hongkongensis. the complete cdna of chtnf was 2457bp in length containing an open reading frame (orf) of 1044bp, a 5' ...201728189340
a molluscan extracellular signal-regulated kinase is involved in host response to immune challenges in vivo and in vitro.extracellular signal-regulated kinases (erks) are a group of highly conserved serine/threonine-specific protein kinases that function as important signaling intermediates in mitogen-activated protein kinase (mapk) pathways, which are involved in a wide variety of cellular activities, including proliferation, inflammation and cytokine production. however, little is known about the roles of this kinase in mollusk immunity. in this study, we identified a molluscan erk homolog (cherk) in the hong ko ...201728159693
a novel p38 mapk indentified from crassostrea hongkongensis and its involvement in host response to immune challenges.p38 mitogen-activated protein kinases (mapks) are conserved serine/threonine-specific kinases that are activated by various extracellular stimuli and play crucial regulatory roles in immunity, development and homeostasis. however, the function of p38s in mollusks, the second most diverse group of animals, is still poorly understood. in this study, a novel molluscan p38 (designated chp38) was cloned and characterized from the hong kong oyster crassostrea hongkongensis. its full-length cdna encode ...201627768933
identification of odontella aurita by rbcl gene sequence - a high antibacterial potential centric marine diatom.the antibacterial potential of centric marine diatoms has been compared against the clinical pathogens and identified the potential diatom by rbcl gene sequencing. totally, five diatoms namely odontella aurita, thalassiosira subtilis, chaetoceros curvisetus, skeletonema costatum and coscinodiscus centralis were isolated from cuddalore coastal waters. the diatoms were morphologically identified and isolated using micro capillary-pipette and serial dilution method. the isolated diatoms were cultur ...201627159722
complicated sea urchin-induced wound infection caused by vibrioalginolyticus and staphylococcus lugdunensis in a 14-year-old boy.wound infections with vibrio alginolyticus, a gram-negative bacterium found in all temperate oceans, are rarely reported. however, a rising incidence of wound infections caused by v. alginolyticus requires better knowledge about this infectious agent.201628348795
antibiotic resistance of vibrio species isolated from sparus aurata reared in italian mariculture.extensive use of antimicrobial agents in finfish farming and the consequent selective pressure lead to the acquisition of antibiotic resistance in aquaculture environment bacteria. vibrio genus represents one of the main pathogens affecting gilthead sea bream. the development of antibiotic resistance by vibrio represents a potential threat to human health by exchange of resistant genes to human pathogens through food chain. the objective of the present study was to conduct a multisite survey on ...201425180847
responses of mytilus galloprovincialis hemocytes to environmental strains of vibrio parahaemolyticus, vibrio alginolyticus, vibrio vulnificus.marine bivalves are exposed to different types of bacteria in the surrounding waters, in particular of the vibrio genus. in the hemocytes of the mussel mytilus spp. immune responses to different vibrios have been largely characterized. however, little information is available on the hemocyte responses to human pathogenic vibrios commonly detected in coastal waters and bivalve tissues that are involved in seafood-borne diseases.201728390964
structure of the sodium-driven flagellar motor in marine vibrio.most bacteria can swim by rotating the flagellum. the basal body of the flagellum is an essential part for this motor function. recent comprehensive analysis of the flagellar basal body structures across bacteria by cryo-electron tomography has revealed that they all share core structures, the rod, and rings: the c ring, m ring, s ring, l ring, and p ring. furthermore, it also has uncovered that in some bacteria, there are extra ring structures in the periplasmic space and outer-membrane. here, ...201728389960
pumilacidin-like lipopeptides derived from marine bacterium bacillus sp. suppress the motility of vibrio alginolyticus.bacterial motility is a crucial factor during the invasion and colonization processes of pathogens, which makes it an attractive therapeutic drug target. here, we isolated a marine bacterium (vibrio alginolyticus 178) from a seamount in the tropical west pacific that exhibits vigorous motility on agar plates and severe pathogenicity to zebrafish. we found that v. alginolyticus 178 motility was significantly suppressed by another marine bacterium bacillus sp. 176 isolated from the same niche. we ...201728389538
enzymatic debridement: is ha-collagenase the right synergy? randomized double-blind controlled clinical trial in venous leg ulcers.the aim of this study is to evaluate the efficacy and safety of a new ointment containing hyaluronic acid and collagenase from non-pathogenic vibrio alginolyticus.201728387882
molecular cloning, characterization and function of a germinal center kinase mst4 gene from litopenaeus vannamei in response to vibrio alginolyticus challenge in tlr-traf6 signaling pathway.the serine/threonine protein kinase mst4 plays multiple roles in the regulation of signaling pathways that govern cellular processes including mitosis, migration, homeostasis, polarity, proliferation, differentiation and apoptosis. here we report the identification and characterization of the full-length sequence of lvmst4 from the shrimp l. vannamei, and investigations into its role in the shrimp's immune response to infection by the pathogenic bacterium vibrio alginolyticus. subcellular locali ...201728377200
homeopathy outperforms antibiotics treatment in juvenile scallop argopecten ventricosus: effects on growth, survival, and immune response.mortality from vibriosis in mollusk production is attributed to pathogenic bacteria, particularly vibrio alginolyticus. use of increasingly potent antibiotics has led to bacterial resistance and increased pathogenicity. alternatives in sanitation, safety, and environmental sustainability are currently under analysis. to-date, homeopathy has been investigated in aquaculture of freshwater fish, but not in marine mollusks. the effect of the homeopathic complexes in the growth, survival, and immune ...201728325219
tumor necrosis factor receptor-associated factor 6 (traf6) participates in peroxinectin gene expression in fenneropenaeus penicillatus.tumor necrosis factor receptor-associated factor 6 (traf6) is an important cytoplasm signal adaptor that mediates signals activated by tumor necrosis factor receptor (tnfr) superfamily and the interleukin-1 receptor/toll-like receptor (il-1/tlr) superfamily. in the study, the full-length cdna of a traf6 homolog (fptraf6) was identified from fenneropenaeus penicillatus. the full-length cdna of fptraf6 is 2033 bp long, with an open reading frame (orf) encoding a putative protein of 594 amino acids ...201728315393
sponge-associated bacteria produce non-cytotoxic melanin which protects animal cells from photo-toxicity.melanin is a photo-protective polymer found in many organisms. our research shows that the bacteria associated with darkly pigmented sponges (haliclona pigmentifera, sigmadocia pumila, fasciospongia cavernosa, spongia officinalis, and callyspongia diffusa) secrete non-cytotoxic melanin, with antioxidant activity that protects animal cells from photo-toxicity. out of 156 bacterial strains screened, 22 produced melanin and these melanin-producing bacteria (mpb) were identified as vibrio spp., prov ...201728315112
instabilities of a rotating helical rod in a viscous fluid.bacteria such as vibrio alginolyticus swim through a fluid by utilizing the rotational motion of their helical flagellum driven by a rotary motor. the flagellar motor is embedded in the cell body and turns either clockwise (cw) or counterclockwise (ccw), which may lead to straight forward or backward swimming, or reorientation of the cell. in this paper we investigate the dynamics of the helical flagellum by adopting the kirchhoff rod theory in which the flagellum is described as a space curve a ...201728297972
immune response and antioxidant status of portunus trituberculatus inoculated with pathogens.the white spot syndrome virus (wssv), vibrio parahaemolyticus and v. alginolyticus are serious epidemic pathogen affecting the cultured portunus trituberculatus and resulted in severe economic losses to local farmers. the immune and antioxidant systems are believed to be closely involved in host responses to pathogens in aerobic animals, including crustaceans. in order to explore such host-pathogen interactions in the early stage of infection in p. trituberculatus, the mrna transcript levels of ...201728235638
molecular detection of the three major pathogenic vibrio species from seafood products and sediments in tunisia using real-time pcr.vibrio spp. have emerged as a serious threat to human health worldwide. v. parahaemolyticus , v. cholerae , and v. vulnificus pose a considerable public health risk in tunisia because they cause sporadic and epidemic foodborne infections associated with the consumption of raw or undercooked contaminated seafood. more recently, toxr-positive v. alginolyticus was also reported to be a potential source of contaminated seafood. a total of 247 samples, including 113 fishes ( labrus viridis , penaeus ...201628221954
vibrio alginolyticus infections in the usa, 1988-2012.vibrio alginolyticus causes soft tissue and bloodstream infection; little systematically collected clinical and epidemiological information is available. in the usa, v. alginolyticus infections are reported to the cholera and other vibrio illness surveillance system. using data from 1988 to 2012, we categorised infections using specimen source and exposure history, analysed case characteristics, and calculated incidence rates using us census bureau data. most (96%) of the 1331 v. alginolyticus i ...201728202099
microrna-100 is involved in shrimp immune response to white spot syndrome virus (wssv) and vibrio alginolyticus this study, we discovered that shrimp mir-100 was up-regulated at 24 h after wssv or vibrio alginolyticus infection, confirming its participation in the innate immune system of shrimp. the anti-mirna oligonucleotide (amo-mir-100) was applied to inhibit the expression of mir-100. after amo-mir-100 treatment, the shrimp was challenged with wssv or v. alginolyticus. the knockdown of mir-100 expression decreased the mortality of wssv-infected shrimp from 24 h to 72 h post-infection and enhanced t ...201728181552
length-dependent flagellar growth of vibrio alginolyticus revealed by real time fluorescent imaging.bacterial flagella are extracellular filaments that drive swimming in bacteria. during motor assembly, flagellins are transported unfolded through the central channel in the flagellum to the growing tip. here, we applied in vivo fluorescent imaging to monitor in real time the vibrio alginolyticus polar flagella growth. the flagellar growth rate is found to be highly length-dependent. initially, the flagellum grows at a constant rate (50 nm/min) when shorter than 1500 nm. the growth rate decays sharply w ...201728098557
designation of pathogenic resistant bacteria in the sparusaurata sea collected in tunisia coastlines: correlation with high performance liquid chromatography-tandem mass spectrometry analysis of antibiotics.vibrio is characterized by a large number of species and some of them are human pathogens causing gastro intestinal and wound infections through the ingestion or manipulation of contaminated fishes including vibrio parahaemolyticus and vibrio alginolyticus. in this study, we reported the phenotypic and molecular characterization of vibrio parahaemolyticus and vibrio alginolyticus strains isolated from wild and farm sea bream (sparus aurata l.) along the tunisian coast from december 2015 to april ...201728062288
characterization and function analysis of interleukin-1 receptor-associated kinase-1 (irak-1) from fenneropenaeus penicillatus.the interleukin-1 receptor-associated kinase-1 (irak-1) is an important adapter protein which links downstream of myd88, and involved in the complex composed of myd88 and traf6 to activate tlrs signaling pathway. in this study, an irak-1 homolog (fpirak-1) was cloned from the red tail shrimp fenneropenaeus penicillatus. the orf of fpirak-1 consisted of 2874 bp encoding a protein of 957 amino acids which contains a death domain (dd) and a catalytic domain of serine/threonine kinases (stkc). homol ...201728025158
potentiometric aptasensing of vibrio alginolyticus based on dna nanostructure-modified magnetic beads.a potentiometric aptasensing assay that couples the dna nanostructure-modified magnetic beads with a solid-contact polycation-sensitive membrane electrode for the detection of vibrio alginolyticus is herein described. the dna nanostructure-modified magnetic beads are used for amplification of the potential response and elimination of the interfering effect from a complex sample matrix. the solid-contact polycation-sensitive membrane electrode using protamine as an indicator is employed to chrono ...201627918423
antifouling activity towards mussel by small-molecule compounds from a strain of vibrio alginolyticus bacterium associated with sea anemone haliplanella sp.mussels are major fouling organisms causing serious technical and economic problems. in this study, antifouling activity towards mussel was found in three compounds isolated from a marine bacterium associated with the sea anemone haliplanella sp. this bacterial strain, called pe2, was identified as vibrio alginolyticus using morphology, biochemical tests, and phylogenetic analysis based on sequences of 16s rrna and four housekeeping genes (rpod, gyrb, rctb, and toxr). three small-molecule compou ...201727880960
season-specific occurrence of potentially pathogenic vibrio spp. on the southern coast of south korea.vibrio species are widely distributed in warm estuarine and coastal environments, and they can infect humans through the consumption of raw and mishandled contaminated seafood. in this study, we aimed to isolate and observe the distribution of enteropathogenic vibrio spp. from environments of the southern coast of south korea over a season cycle. a total of 10,983 isolates of vibrio spp. were obtained from tidal water and mud samples over a 1-year period from five sampling sites along the southw ...201727836844
the upregulation of immune responses in tyrosine hydroxylase (th) silenced litopenaeus vannamei.catecholamines (cas) play a crucial role in maintaining physiological and immune homeostasis in invertebrates and vertebrates under stressful conditions. tyrosine hydroxylase (th) is the first and rate-limiting enzyme in ca synthesis. to develop an effective ca-related immunological defense system against stress and pathogen infection, various criteria, were evaluated in th double-stranded (ds) rna-injected white shrimp, litopenaeus vannamei. specifically, the relative transcript quantification ...201727825820
white shrimp litopenaeus vannamei that have received fucoidan exhibit a defense against vibrio alginolyticus and wssv despite their recovery of immune parameters to background levels.white shrimp litopenaeus vannamei receiving fucoidan at 2, 6, and 10 μg g(-1) after 0-144 h or 0-120 h were examined for immune parameters (haemograms, phenoloxidase activity, respiratory burst, and superoxide dismutase activity), proliferation of haemocyte in the haematopoietic tissue (hpt), gene expression, and phagocytic activity and clearance efficiency to vibrio alginolyticus. immune parameters and mitotic index of hpt increased after 3-24 h, reached their maxima after 48-72 h, and returned ...201627815206
dietary administration of saponin stimulates growth of the swimming crab portunus trituberculatus and enhances its resistance against vibrio alginolyticus infection.the immunostimulatory role of dietary saponins on swimming crabs was investigated under field conditions. portunus trituberculatus were fed diets enriched with quillaja saponin (qs) at 150, 300 and 450 mg kg(-1). they had an enhanced growth rate and increased resistance against vibrio alginolyticus compared to crabs not fed with qs. significant effects were observed on the average body weight, percentage weight gain and specific growth rate (p < 0.05). total hemocyte and hyaline cell counts of p ...201627815204
identification, characterization and expression analysis of tumor suppressor protein p53 from pufferfish (takifugu obscurus) after the vibrio alginolyticus challenge.the tumor suppressor protein p53 plays a critical role in cell cycle, apoptosis and dna repair. in this study, the full-length pufferfish p53 (pf-p53) was obtained, containing an open reading frame of 1095 bp, a 5'utr of 157 bp and a 3'utr of 285 bp with a poly (a) tail. the pf-p53 encoded a polypeptide of 364 amino acids with a theoretical isoelectric point of 8.03 and predicted molecular weight of 40.6 kda. pf-p53 was ubiquitously expressed in various tissues with a high-level expression in ki ...201627815196
mutational analysis and overproduction effects of motx, an essential component for motor function of na+-driven polar flagella of vibrio.the bacterial flagellar motor is a rotary motor complex composed of various proteins. the motor contains a central rod, multiple ring-like structures and stators. the na(+)-driven polar flagellar motor of the marine bacterium vibrio alginolyticus has a specific ring, called the 't-ring', which consists of two periplasmic proteins, motx and moty. the t-ring is essential for assembly of the torque-generating unit, the poma/pomb stator complex, into the motor. to investigate the role of the t-ring ...201627803076
characterization of potential lytic bacteriophage against vibrio alginolyticus and its therapeutic implications on biofilm dispersal.vibrio alginolyticus is a leading cause of vibriosis, presenting opportunistic infections to humans associated with raw seafood contamination. at present, phage therapy that acts as an alternative sanitizing agent is explored for targeting v. alginolyticus. the study outcome revealed that the phage vp01 with its extreme lytic effect showed a high potential impact on the growth of v. alginolyticus as well as biofilm formation. electron microscopy revealed the phage resemblance to myoviridae, base ...201627793690
molecular cloning and characterization of pten in the orange-spotted grouper (epinephelus coioides).pten is a key tumor suppressor gene that can play a regulatory role in the cellular proliferation, survival and apoptosis. in this study, the full-length pten (ecpten) was obtained, containing a 5'utr of 745 bp, an orf of 1269 bp and a 3'utr of 106 bp. the ecpten gene encoded a polypeptide of 422 amino acids with an estimated molecular mass of 49.14 kda and a predicted isoelectric point (pi) of 6.34. the deduced amino acid sequence analysis showed that ecpten comprised the conserved residues and ...201627725262
the cooperative expression of heat shock protein 70 kd and 90 kd gene in juvenile larimichthys crocea under vibrio alginolyticus stress.heat shock proteins (hsps) play significant roles in the immune response of fish in defending against diverse environmental threats or stresses. in this study, two complete hsp70 and hsp90 genes of larimichthys crocea (designated as lychsp70 and lychsp90) were identified and characterized (genbank accession no. kt456551 and kt456552). the complete open reading frame (orf) fragments of lychsp70 and lychsp90 were 1917 bp and 2151 bp, encoding 638 and 716 amino acids residues respectively. many sig ...201627678510
identification of a novel class b scavenger receptor homologue in portunus trituberculatus: molecular cloning and microbial ligand binding.class b scavenger receptors (srbs), which are present in mammals and insects, have been implicated in a wide range of functions. herein, a novel srb homologue, ptsrb, was cloned from the swimming crab, portunus trituberculatus. ptsrb has 538 amino acid residues, and it consists of two transmembrane regions, a large extracellular loop, and two intracellular tails. a phylogenetic analysis showed that ptsrb distinctly clustered with marsupenaeus japonicas srb-1 and most drosophila srb homologues, i ...201627633673
hubp, a polar landmark protein, regulates flagellar number by assisting in the proper polar localization of flhg in vibrio alginolyticus.the marine bacterium vibrio alginolyticus has a single polar flagellum, the number of which is regulated positively by flhf and negatively by flhg. flhf is intrinsically localized at the cell pole, whereas flhg is localized there through putative interactions with the polar landmark protein hubp. here we focused on the role of hubp in the regulation of flagellar number in v. alginolyticus deletion of hubp increased the flagellar number and completely disrupted the polar localization of flhg. it ...201627573015
molecular characterization and function of the prohibitin2 gene in litopenaeus vannamei responses to vibrio alginolyticus.prohibitin2 (phb2), a potential tumor suppressor protein, plays important roles in inhibition of cell cycle progression, transcriptional regulation, apoptosis and the mitochondrial respiratory chain. to explore its potential roles in crustaceans' immune responses we have identified and characterized lvphb2, a 891 bp gene encoding a 297 amino acids protein in the shrimp litopenaeus vannamei. expression analyses showed that lvphb2 is expressed in all examined tissues, and largely present in cytopl ...201727756688
characterization of vibrio parahaemolyticus and its specific phage from shrimp pond in palk strait, south east coast of india.phage therapy is an alternative and eco-friendly biocontrol agent to prevent and control multidrug resistant bacteria in the aquatic system. the aim of this study is to isolate and characterize the vibrio parahaemolyticus and its potential lytic phage from penaeus monodon growing-out by rearing in shrimp ponds in palk strait, south east coast of india. the conventional phenotypic characteristics and molecular identification was confirmed using 16s rrna sequence and to determine the antibiotic re ...201627697363
complete genome sequence of vibrio alginolyticus zj-t.vibrio alginolyticus is a ubiquitous gram-negative bacterium which is normally distributed in the coastal and estuarine environments. it has been suggested to be an opportunistic pathogen to both marine animals and humans, here, the completed genome sequence of v. alginolyticus zj-t was determined by illumina high-throughput sequencing.201627587824
studies on the mechanism of bacterial flagellar rotation and the flagellar number regulation.many motile bacteria have the motility organ, the flagellum. it rotates by the rotary motor driven by the ion-motive force and is embedded in the cell surface at the base of each flagellar filament. many researchers have been studying its rotary mechanism for years, but most of the energy conversion processes have been remained in mystery. we focused on the flagellar stator, which works at the core process of energy conversion, and found that the periplasmic region of the stator changes its conf ...201627581279
filtering activity on a pure culture of vibrio alginolyticus by the solitary ascidian styela plicata and the colonial ascidian polyandrocarpa zorritensis: a potential service to improve microbiological seawater quality economically.we investigated and compared, by laboratory experiments, the filter-feeding activity on bacteria by the solitary ascidian styela plicata and the colonial ascidian polyandrocarpa zorritensis. clearance rates and retention efficiencies were estimated by using, as only food source, the bacterial species vibrio alginolyticus selected on account of its importance in aquaculture pathogenicity. the cmax was 1.4±0.17lh(-1)g(-1) dw for s. plicata and 1.745lh(-1)g(-1) dw for p. zorritensis. the highest re ...201627552728
chromatin immunoprecipitation sequencing technology reveals global regulatory roles of low-cell-density quorum-sensing regulator apha in the pathogen vibrio alginolyticus.quorum sensing (qs) is an important regulatory system in virulence expression and environmental adaptation in bacteria. the master qs regulators (mqsr) luxr and apha reciprocally control qs gene expression in vibrios. however, the molecular basis for the regulatory functions of apha remains undefined. in this study, we characterized its regulatory roles in vibrio alginolyticus, an important zoonotic pathogen causing diseases in marine animals as well as in humans. apha is involved in the motilit ...201627551022
antimicrobial susceptibility of vibrio alginolyticus isolated from oyster in korea.pathogenic vibrio alginolyticus, a cause of severe infection in shellfish, as well as in humans, has been found at high frequency around all coastal areas of korea. the aim of this study was to determine the occurrence of v. alginolyticus, to identify the strains isolated from oysters in west sea, and to investigate their antimicrobial resistance profiles. biochemical analyses of the 90 initially recovered presumptive v. alginolyticus colonies indicated that 16 isolates were v. alginolyticus. pc ...201627543129
cloning and characterization of tyrosine hydroxylase (th) from the pacific white leg shrimp litopenaeus vannamei, and its expression following pathogen challenge and hypothermal stress.tyrosine hydroxylase (th) belongs to the biopterin-dependent aromatic amino acid hydroxylase enzyme family, and it represents the first and rate-limiting step in the synthesis of catecholamines that are required for physiological and immune process in invertebrates and vertebrates. cloned litopenaeus vannamei th (lvth), containing a short alpha helix domain, a catalytic core, a regulatory domain, a phosphorylation site and two potential n-linked glycosylation sites as presented in vertebrate and ...201627514780
a novel biomarker for marine environmental pollution of hsp90 from mytilus coruscus.heat shock protein 90 (hsp90) is a conserved molecular chaperone contributing to cell cycle control, organism development and the proper regulation of cytosolic proteins. the full-length hsp90 cdna of mytilus coruscus (mchsp90, kt946644) was 2420bp, including an orf of 2169bp encoding a polypeptide of 722 amino acids with predicted pi/mw 4.89/83.22kda. blastp analysis and phylogenetic relationship strongly suggested mchsp90 was a member of hsp90 family, and it was highly conserved with other kno ...201627491367
collagenase from vibrio alginolyticus cultures: experimental study and clinical perspectives.purpose the purpose of this study is to examine the activity of collagenase from cultures of vibrio alginolyticus as in vitro as in biological samples and to evaluate clinical perspectives of this product about the treatment of fibroproliferative diseases like dupuytren's contracture. methods the experimental part of the study has been divided in 2 stages. in the first stage, the collagenase has been produced in laboratory, assessing its purity, verifying the in vitro degradation of collagen by ...201627448558
spirulina elicits the activation of innate immunity and increases resistance against vibrio alginolyticus in shrimp.the effect of spirulina dried powder (sdp) on the immune response of white shrimp litopenaeus vannamei was studied in vitro and in vivo. incubating shrimp haemocytes in 0.5 mg ml(-1) sdp caused the degranulation of haemocytes and a reduction in the percentage of large cells within 30 min. shrimp haemocytes incubated in 1 mg ml(-1) sdp significantly increased their phenoloxidase (po) activity, serine proteinase activity, and respiratory burst activity (rb, release of superoxide anion). a recombin ...201627368541
an alpha-2 macroglobulin in the pearl oyster pinctada fucata: characterization and function in hemocyte phagocytosis of vibrio alginolyticus.alpha-2 macroglobulin (α2m) is a ubiquitous protease inhibitor and considered to be an evolutionarily conserved constituent of innate host defence system. here, an α2m gene (designated as pfα2m) was obtained from the pearl oyster pinctada fucata by rt-pcr, pcr walking and rapid amplification of cdna ends (race). the pfα2m cdna consists of 6394 bp with an open reading frame (orf) of 5745 bp encoding a protein of 1914 amino acids with a 19 residues signal peptide. pfα2m sequence contains three put ...201627346151
[post nearly drowning vibrio alginolyticus septicemia acquired in reunion (indian ocean)].abstractwe report the first case of vibrio alginolyticus septicemia in the indian ocean (reunion island), in a patient (70-year-old-man) with multiple underlying conditions, following a nearly drowning in the lagoon of reunion. from now on, v. alginolyticus should be considered as a possible agent of septicemia in the indian ocean, particularly following marine activities.201627325174
isolation and characterization of agar-digesting vibrio species from the rotten thallus of gracilariopsis heteroclada zhang et xia.gracilariopsis heteroclada zhang et xia (gracilariaceae, rhodophyta) is one of the most studied marine seaweeds due to its economic importance. this has been cultivated extensively on commercial scale in the philippines and other asian countries. however, sustainable production of g. heteroclada in the philippines could not be maximized due to the occurrence of rotten thallus disease. thus, isolation and characterization of agar-digesting bacteria from the rotten thalli of g. heteroclada was con ...201627285614
a σe-mediated temperature gauge controls a switch from luxr-mediated virulence gene expression to thermal stress adaptation in vibrio vibrios, the expression of virulence factors is often controlled by luxr, the master quorum-sensing regulator. here, we investigate the interplay between luxr and σe, an alternative sigma factor, during the control of virulence-related gene expression and adaptations to temperature elevations in the zoonotic pathogen vibrio alginolyticus. an rpoe null v. alginolyticus mutant was unable to adapt to various stresses and was survival-deficient in fish. in wild type v. alginolyticus, the expressi ...201627253371
complete genome sequence of vibrio alginolyticus atcc 33787(t) isolated from seawater with three native megaplasmids.vibrio alginolyticus, an opportunistic pathogen, is commonly associated with vibriosis in fish and shellfish and can also cause superficial and ear infections in humans. v. alginolyticus atcc 33787(t) was originally isolated from seawater and has been used as one of the type strains for exploring the virulence factors of marine bacteria and for developing vaccine against vibriosis. here we sequenced and assembled the whole genome of this strain, and identified three megaplasmids and three type v ...201627211073
genome-wide detection of predicted non-coding rnas related to the adhesion process in vibrio alginolyticus using high-throughput sequencing.the ability of bacteria to adhere to fish mucus can be affected by environmental conditions and is considered to be a key virulence factor of vibrio alginolyticus. however, the molecular mechanism underlying this ability remains unclear. our previous study showed that stress conditions such as exposure to cu, pb, hg, and low ph are capable of reducing the adhesion ability of v. alginolyticus. non-coding rnas (ncrnas) play a crucial role in the intricate regulation of bacterial gene expression, t ...201627199948
saharan dust nutrients promote vibrio bloom formation in marine surface waters.vibrio is a ubiquitous genus of marine bacteria, typically comprising a small fraction of the total microbial community in surface waters, but capable of becoming a dominant taxon in response to poorly characterized factors. iron (fe), often restricted by limited bioavailability and low external supply, is an essential micronutrient that can limit vibrio growth. vibrio species have robust metabolic capabilities and an array of fe-acquisition mechanisms, and are able to respond rapidly to nutrien ...201627162369
whole-genome sequence of vibrio alginolyticus isolated from the mucus of the coral fungia danai in the andaman sea, india.vibrio alginolyticus, a halophilic gram-negative bacterium, which is found in temperate marine and estuarine environments, is known to cause infections in humans and other organisms. we sequenced the genome of sulfamethoxazole-trimthoprim-positive v. alginolyticus strain 4-19 isolated from the mucus of the coral fungia danai in the andaman sea, india.201627151803
beta-1,3-1,6-glucan modulate the non-specific immune response to enhance the survival in the vibrio alginolyticus infection of taiwan abalone (haliotis diversicolor supertexta).this research aims to investigate the non-specific immune response of taiwan abalone (haliotis diversicolor supertexta) which was treated with the beta-1,3-1,6-glucan to be observed in the survival impact after the vibrio alginolyticus infection. the non-specific immune and physiological response of superoxide anion radical (o2(-)), phenoloxidase (po), phagocytic index (pi), phagocytic rate (pr) and lucigenin-chemiluminescence for reactive oxygen intermediates (rois) were enhanced via in-vitro e ...201627150049
comparative genomic analysis of six new-found integrative conjugative elements (ices) in vibrio alginolyticus.vibrio alginolyticus is ubiquitous in marine and estuarine environments. in 2012-2013, sxt/r391-like integrative conjugative elements (ices) in environmental v. alginolyticus strains were discovered and found to occur in 8.9 % of 192 v. alginolyticus strains, which suggests that v. alginolyticus may be a natural pool possessing resourceful ices. however, complete ice sequences originating from this bacterium have not been reported, which represents a significant barrier to characterizing the ice ...201627145747
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