PMID(sorted ascending)
screening and selection of stress resistant lactobacillus spp. isolated from the marine oyster (crassostrea gigas).we attempted to isolate lactobacillus spp. from the marine oyster (crassostrea gigas) and select stress resistant strains for development of a future marine aquaculture feed adjuvant. a total of 83 lactobacilli strains were isolated from oyster. they were all gram-positive, rod-shaped and catalase-negative. by performing a stress resistance assay, we selected eighteen isolates. based on 16s rrna gene sequencing, lactobacillus paracasei was the most prevalent species among the selected isolates. ...201020633689
evaluation of immune response and resistance to diseases in tiger shrimp, penaeus monodon fed with biofilm of vibrio alginolyticus.immune response in juvenile tiger shrimp, penaeus monodon fed with biofilm (bf) and free cells (fc) of vibrio alginolyticus was studied by evaluating the hemocyte count, phenoloxidase activity and antibacterial activity. the above immune responses were higher in bf fed shrimp than that in fc fed or control shrimp. among the different doses of bf of v. alginolyticus tested, 10(9) cfu g(-1) shrimp day(-1) for two weeks could evoke higher immune response. bf fed shrimp were more resistant to inject ...201020638481
identification of vaccine candidates from differentially expressed outer membrane proteins of vibrio alginolyticus in response to nacl and iron limitation.vibrio alginolyticus is the etiological agent that causes great losses in aquacultures and clinical emanating cases in humans. identification of highly efficient vaccine candidates to control v. alginolyticus infection has been highly concerned since vaccines offer a powerful approach to provide efficient protection from bacterial infections. in the present study, we firstly investigated the altered outer membrane proteins (om proteins) of v. alginolyticus in response to nacl concentrations and ...201020659563
expression, purification, and characterization of thermolabile hemolysin (tlh) from vibrio alginolyticus.hemolysin is a putative pathogenicity factor in many bacterial pathogens. in this study, a dna fragment containing the open reading frame (1254 bp) of the thermolabile hemolysin gene (tlh) from vibrio alginolyticus v05 was amplified and cloned into the expression plasmid pet-24d(+). the deduced amino acid sequence of the thermolabile hemolysin (tlh) shared 94 and 83% identity with the lecithin-dependent hemolysin (ldh)/tlh of v. parahaemolyticus and v. harveyi thermolabile hemolysin (vhh), respe ...201020662368
comparative genomic analyses identify the vibrio harveyi genome sequenced strains baa-1116 and hy01 as vibrio campbellii.three notable members of the harveyi clade, vibrio harveyi, vibrio alginolyticus and vibrio parahaemolyticus, are best known as marine pathogens of commercial and medical import. in spite of this fact, the discrimination of harveyi clade members remains difficult due to genetic and phenotypic similarities, and this has led to misidentifications and inaccurate estimations of a species' involvement in certain environments. to begin to understand the underlying genetics that complicate species leve ...201020686623
brichos domain-containing leukocyte cell-derived chemotaxin 1-like cdna from disk abalone haliotis discus discus.a brichos domain-containing leukocyte cell-derived chemotaxin 1-like cdna was cloned from the disk abalone (haliotis discus discus) and designated as ablect-1. a full-length (705 bp) of ablect-1 cdna was composed of a 576 bp open reading frame that translates into a putative peptide of 192 amino acids. deduced amino acid sequence of ablect-1 had 15.5- and 27.8% identity and similarity to human lect-1, respectively. quantitative real-time pcr analysis results showed that the mrna of ablect-1 was ...201020688173
zernike phase contrast cryo-electron tomography of sodium-driven flagellar hook-basal bodies from vibrio alginolyticus.vibrio alginolyticus use flagella to swim. a flagellum consists of a filament, hook and basal body. the basal body is made up of a rod and several ring structures. this study investigates the structure of the t ring which is a unique component of the v. alginolyticus sodium ion-driven flagellar basal body. using zernike phase contrast (zpc) cryo-electron tomography, we compared the 3d structures of purified hook-basal bodies (hbb) from a wild-type strain (kk148) and a deletion mutant lacking mot ...201120705140
the flagellar basal body-associated protein flgt is essential for a novel ring structure in the sodium-driven vibrio vibrio alginolyticus, the flagellar motor can rotate at a remarkably high speed, ca. three to four times faster than the escherichia coli or salmonella motor. here, we found a vibrio-specific protein, flgt, in the purified flagellar basal body fraction. defects of flgt resulted in partial fla⁻ and mot⁻ phenotypes, suggesting that flgt is involved in formation of the flagellar structure and generating flagellar rotation. electron microscopic observation of the basal body of δflgt cells reveale ...201020729351
first molluscan transcription factor activator protein-1 (ap-1) member from disk abalone and its expression profiling against immune challenge and tissue injury.the regulation of transcriptional activation is an essential and critical point in gene expression. in this study, we describe a novel transcription factor activator protein-1 (ap-1) gene from disk abalone haliotis discus discus (abap-1) for the first time in mollusk. it was identified by homology screening of an abalone normalized cdna library. the cloned abap-1 consists of a 945 bp coding region that encodes a putative protein containing 315 amino acids. the abap-1 gene is composed of a charac ...201020732431
dietary medicinal plant extracts improve growth, immune activity and survival of tilapia oreochromis mossambicus.the effects of supplementing diets with acetone extract (1% w/w) from four medicinal plants (bermuda grass cynodon dactylon, h(1), beal aegle marmelos, h(2), winter cherry withania somnifera, h(3) and ginger zingiber officinale, h(4)) on growth, the non-specific immune response and ability to resist pathogen infection in tilapia oreochromis mossambicus were assessed. in addition, the antimicrobial properties of the extract were assessed against vibrio alginolyticus, vibrioparahaemolyticus, vibri ...200920735646
a multidomain galectin involved in innate immune response of pearl oyster pinctada fucata.galectins could specifically bind to β-galactoside residues and play crucial roles in innate immune responses of vertebrates and invertebrates. in this study, the cdna of a galectin with multiple carbohydrate-recognition domains (crds) was cloned from pearl oyster pinctada fucata (designated as pogal). pogal cdna was 2138bp long and consisted of a 5'-untranslated region (utr) of 120bp, a 3'-utr of 350bp with two cytokine rna instability motifs (attta), and an open reading frame (orf) of 1668bp e ...201120813129
immunoglobulin m gene expression analysis of orange-spotted grouper, epinephelus coioides, following heat shock and vibrio alginolyticus challenge.the present study aimed to examine the expression of immunoglobulin m (igm) gene in orange-spotted grouper (epinephelus coioides) following thermal stress, bacterial infection, and immunization with formalin-killed vibrio alginolyticus, a kind of bacterial pathogen that causes septicemia. in heat shock experiments, twenty-five healthy orange-spotted grouper were kept in tanks with seawater at 37±0.5°c for one hour heat-shock treatment, and then returned to 27±0.5°c seawater tanks. in bacterial c ...201020816805
litopenaeus vannamei tumor necrosis factor receptor-associated factor 6 (traf6) responds to vibrio alginolyticus and white spot syndrome virus (wssv) infection and activates antimicrobial peptide genes.tumor necrosis factor receptor (tnfr)-associated factor 6 (traf6) is a key signaling adaptor protein not only for the tnfr superfamily but also for the interleukin-1 receptor/toll-like receptor (il-1/tlr) superfamily. to investigate traf6 function in invertebrate innate immune responses, litopenaeus vannamei traf6 (lvtraf6) was identified and characterized. the full-length cdna of lvtraf6 is 2823bp long, with an open reading frame (orf) encoding a putative protein of 594 amino acids, including a ...201120816892
crystal structure and mutational analysis of aminoacylhistidine dipeptidase from vibrio alginolyticus reveal a new architecture of m20 metallopeptidases.aminoacylhistidine dipeptidases (pepd, ec belong to the family of m20 metallopeptidases from the metallopeptidase h clan that catalyze a broad range of dipeptide and tripeptide substrates, including l-carnosine and l-homocarnosine. homocarnosine has been suggested as a precursor for the neurotransmitter γ-aminobutyric acid (gaba) and may mediate the antiseizure effects of gabaergic therapies. here, we report the crystal structure of pepd from vibrio alginolyticus and the results of mut ...201020819954
molecular characterization and expression analysis of extracellular copper-zinc superoxide dismutase gene from swimming crab portunus extracellular cuznsod cdna was cloned from the haemocytes of swimming crab portunus trituberculatus by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna end (race) method. analysis of the nucleotide sequence revealed that the eccuznsod full-length cdna consisted of 965 bp with an open reading frame of 579 bp. it encoded a polypeptide of 192 amino acids which had a predicted molecular weight of 20.0 kda and with an estimated pi of 6.23. the deduced amin ...201120848212
cloning and characterization of two ferritin subunit genes from bay scallop, argopecten irradians (lamarck 1819).we have cloned two full-length cdnas from two ferritin genes (aifer1 and aifer2) of the bay scallop, argopecten irradians (lamarck 1819). the cdnas are 1,019 and 827 bp in length and encode proteins of 171 and 173 amino acids, respectively. the 5' utr of each contains a conserved iron response element (ire) motif. sequence analyses reveal that both proteins belong to the h-ferritin family with seven conserved amino acids in the ferroxidase center. highest expression of aifer1 is found in the man ...201020878240
diet enriched with mushroom phellinus linteus extract enhances the growth, innate immune response, and disease resistance of kelp grouper, epinephelus bruneus against vibriosis.the effect of diet supplemented with phellinus linteus fed for 30 days was investigated in grouper epinephelus bruneus challenged with vibrio anguillarum, vibrio harveyi, vibrio alginolyticus, and vibrio carchariae; infected and treated fish had a significantly higher percent weight gain and feed efficiency. in groups fed with enriched diet and challenged with v. anguillarum and v. harveyi the mortality rate declined with a consequent rise in survival rate than with other pathogens. on the other ...201020883799
an acute gastroenteritis outbreak of vibrio parahaemolyticus o4:k55 in nursing college, thailand.a cluster of acute gastroenteritis among nursing students was noticed on 13th september 2005. between 13th and 17th september 2005, a retrospective cohort study was then conducted to identify the most likely cause of gastroenteritis at a nursing college in bangkok, thailand. self-administered questionnaires, interviews, environmental investigations, and rectal swabs from all participants were carried out. in the investigation, 98.9% female nursing students were investigated and had completed the ...201020962724
transcriptional regulation of extracellular copper zinc superoxide dismutase from white shrimp litopenaeus vannamei following vibrio alginolyticus and wssv infection.the cdna encoding an extracellular copper zinc superoxide dismutase (lvecsod) was cloned from the hepatopancreas of white shrimp litopenaeus vannamei. it consisted of 915 bp nucleotides with an open reading frame corresponding to a deduced protein of 178 amino acids. the lvecsod contains a putative signal peptide of 16 amino acids, two potential n-linked glycosylation sites (n(115)gta and n(135)itg) and a copper zinc superoxide dismutase family signature sequence (g(162)nagarvaccti(173)). it was ...201020974259
identification of outer membrane proteins of vibrio parahaemolyticus and vibrio alginolyticus altered in response to γ-irradiation or long-term starvation.vibrio parahaemolyticus and vibrio alginolyticus were subjected to γ-irradiation (0.5 kgy) or starvation by incubation for 8 months in seawater to study modifications in their outer membrane protein patterns. after treatment, outer membrane protein profiles of starved or γ-irradiated bacteria were found to be altered when analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page). altered proteins were identified by mass spectrometry (ms and ms/ms) and analyses revealed tha ...201021035543
autophagy is induced by the type iii secretion system of vibrio alginolyticus in several mammalian cell lines.vibrio alginolyticus is a gram-negative bacterium and has been recognized as an opportunistic pathogen in marine animals as well as humans. here, we further characterized a cell death mechanism caused by this bacterium in several mammalian cell lines. the t3ss of v. alginolyticus killed hela cells by a very similar cell cytolysis mechanism in fish cells, as evidenced by cell rounding and ldh release; however, dna fragmentation was not observed. further studies showed that caspase-1 and caspase-3 ...201021046072
galectin-1 (ofglec-1) from rock bream (oplegnathus fasciatus): identification, characterization, gene expression and functional properties.lectins may function as pattern-recognition receptors (prrs) and play important roles in immune responses. in this study, a novel galectin-1 (designated ofglec-1) gene was identified from rock bream, oplegnathus fasciatus, cdna library. the complete cdna sequence of the ofglec-1 gene is 993 base pairs in length, and encodes 135 amino acids. ofglec-1 shares a highly conserved carbohydrate recognition domain with galectin-1 from other fishes. the mrna expressions of ofglec-1 in healthy and bacteri ...201021073891
a polyphasic approach for the differentiation of environmental vibrio isolates from temperate waters.climate change and marine traffic lead to changing species communities in the oceans. due to increasing seawater temperatures, pathogenic vibrio species could become significant even in temperate waters. we classified mesophilic vibrio isolates from the german bight (north sea) using a polyphasic approach with special emphasis on vibrio parahaemolyticus. matrix-assisted laser desorption/ionization time-of-flight ms was used as a primary screen to classify isolates, 16s rrna gene and rpob gene se ...201021118277
molluscan death effector domain (ded)-containing caspase-8 gene from disk abalone (haliotis discus discus): molecular characterization and expression analysis.the caspase family represents aspartate-specific cysteine proteases that play key roles in apoptosis and immune signaling. in this study, we cloned the first death effector domain (ded)-containing molluscan caspase-8 gene from disk abalone (haliotis discus discus), which is named as hdcaspase-8. the full-length hdcaspase was 2855 bp, with a 1908 bp open reading frame encoding 636 amino acids. the hdcaspase-8 had 72 kda predicted molecular mass with an estimated isoelectric point (pi) of 6.0. the ...201021130887
development of a single base extension-tag microarray for the detection of pathogenic vibrio species in this study, a single base extension-tag array on glass slides (sbe-tags) microarray was established to detect the seven leading seafood-borne pathogens, including vibrio parahaemolyticus, vibrio cholerae, vibrio vulnificus, vibrio mimicus, vibrio alginolyticus, vibrio anguillarum, and vibrio harveyi. three multiplex pcr assays were developed to specifically target the following species with individual gene markers, which are aads, tdh, and trh for v. parahaemolyticus; col, toxr, and vvh for v ...201021161212
two flagellar stators and their roles in motility and virulence in pseudomonas syringae pv. tabaci 6605.the motor proteins around the flagellar basal body consist of two cytoplasmic membrane proteins, mota and motb, and function as a complex that acts as the stator to generate the torque that drives rotation. genome analysis of several pseudomonas syringae pathovars revealed that there are two sets of genes encoding motor proteins: motab and motcd. deduced amino acid sequences for mota/b and motc/d showed homologies to the h(+)-driven stator from escherichia coli and na(+)-driven stator from vibri ...201021165649
identification of a luxo-regulated extracellular protein pep and its roles in motility in vibrio alginolyticus.the fish pathogen vibrio alginolyticus virulence factors and regulation mechanism are barely known except the quorum sensing regulated extracellular products. in the present study, the cleavable isotope-coded affinity tags (cicats) were used to analyze the differentially expressed extracellular proteins of v. alginolyticus wild-type and a δluxo mutant, and a putative peptidase pep was identified. quantitative real-time reverse transcription pcr confirmed that the transcription of pep was down-re ...201021167274
three isoforms of anti-lipopolysaccharide factor identified from eyestalk cdna library of swimming crab portunus trituberculatus.anti-lipopolysaccharide factors (alfs), as the potent antimicrobial peptides, can bind and neutralize lipopolysaccharide (lps) and exhibit broad spectrum antimicrobial activities. in this study, three isoforms of the alf homologues (ptesalf1-3) were identified from eyestalk cdna library of swimming crab portunus trituberculatus. the full-length cdna sequences of ptesalf1, 2 and 3 were 1138, 1052 and 1057 bp encoding 92, 108 and 123 amino acids, respectively. ptesalf1-3 contained two conserved cy ...201021168510
functional characterization of vibrio alginolyticus twin-arginine translocation system: its roles in biofilm formation, extracellular protease activity, and virulence towards fish.the bacterial twin-arginine translocation (tat) system contributes to translocate folded proteins and plays pleiotropic roles in growth, motility, and the secretion of some virulent factors. in this study, the authors identified the tat gene cluster in fish pathogen vibrio alginolyticus and explored its roles in pathogenesis toward fish. vibrio alginolyticus tat mutants showed growth deficiency in tmao medium, while the complement strain restored the ability to grow in the medium, demonstrating ...201021170534
[cloning and expression pattern analysis of a lipopolysaccharide -and beta-1,3-glucan-binding protein in portunus trituberculatus].the lipopolysaccharide -and beta-1,3-glucan-binding protein (lgbp) is a pattern recognition receptor, which is fundamental for the innate immune response of crustaceans. a lgbp gene was cloned from the haemocytes of portunus trituberculatus using smart race methods. the full-length lgbp cdna (1 378 bp) had a 1 095 bp open reading frame encoding a protein of 365 amino acid residues including a 16 amino acid residues signal peptide, a 138 bp 5' untranslated region (utr) and a 144 bp untranslated r ...201021174347
differential expression of lvhsp60 in shrimp in response to environmental stress.previous studies showed that heat-shock protein 60 (hsp60) was known to function as a molecular chaperone and is an important factor in the innate immune system in mammals. however, little was known about the physiological relevance of hsp60 in marine invertebrates. this study focuses on long-term monitoring of the differential expression of lvhsp60 in shrimp litopenaeus vannamei in response to environmental stress. the thermal aggregation assay elucidated that lvhsp60 was an effective chaperone ...201021182965
implications of three-step swimming patterns in bacterial chemotaxis.we recently found that marine bacteria vibrio alginolyticus execute a cyclic three-step (run-reverse-flick) motility pattern that is distinctively different from the two-step (run-tumble) pattern of escherichia coli. how this novel, to our knowledge, swimming pattern is regulated by cells of v. alginolyticus is not currently known, but its significance for bacterial chemotaxis is self-evident and will be delineated herein. using a statistical approach, we calculated the migration speed of a cell ...201121190654
f-type lectin involved in defense against bacterial infection in the pearl oyster (pinctada martensii).in invertebrates and vertebrates, carbohydrate-binding proteins (lectins) play an important role in innate immunity against microbial invasion. in the present study, we report the cloning of an f-type lectin (designated as pmf-lectin) from pearl oyster (pinctada martensii) using a combination of expression sequence tag (est) analysis and rapid amplification of cdna ends (race) pcr. the full-length cdna of pmf-lectin contains an open reading frame (orf) of 579 bp coding for192 amino acids. the de ...201021195768
protection of red snapper (lutjanus sanguineus) against vibrio alginolyticus with a dna vaccine containing flagellin flaa gene.the main aims of this study were to construct a dna vaccine containing flagellin flaa gene from vibrio alginolyticus strain hy9901 and to explore the potential application of pcdna-flaa as a dna vaccine candidate for red snapper (lutjanus sanguineus).201121198695
conversion of mono-polar to peritrichous flagellation in vibrio alginolyticus.precise regulation of the number and positioning of flagella are critical in order for the mono-polar-flagellated bacterium vibrio alginolyticus to swim efficiently. it has been shown that, in v. alginolyticus cells, the putative gtpase flhf determines the polar location and production of flagella, while the putative atpase flhg interacts with flhf, preventing it from localizing at the pole, and thus negatively regulating the flagellar number. in fact, no δflhf cells have flagella, while a very ...201121204943
from the cover: bacterial flagellum as a propeller and as a rudder for efficient chemotaxis.we investigate swimming and chemotactic behaviors of the polarly flagellated marine bacteria vibrio alginolyticus in an aqueous medium. our observations show that v. alginolyticus execute a cyclic, three-step (forward, reverse, and flick) swimming pattern that is distinctively different from the run-tumble pattern adopted by escherichia coli. specifically, the bacterium backtracks its forward swimming path when the motor reverses. however, upon resuming forward swimming, the flagellum flicks and ...201121205908
phylogenetic and evolutionary analysis of vibrio parahaemolyticus and vibrio alginolyticus isolates based on toxr gene sequence.the vibrio genus contains a large number of closely related bacterial species differing, in some cases, less than 1% in 16s rrna gene sequence. the present study evaluated the usefulness of toxr gene for phylogenetic and evolution analysis on vibrio isolates of environmental or clinical origin belonging to the two closely related species v. parahaemolyticus and v. alginolyticus. the phylogenetic analysis based on toxr gene, contrary to 16s rrna gene, allowed a clear differentiation of the isolat ...201021213595
microarray analysis of gene expression in disk abalone haliotis discus discus after bacterial this study, we investigated the gene expression profiling of disk abalone, haliotis discus discus challenged by a mixture of three pathogenic bacteria vibrio alginolyticus, vibrio parahemolyticus, and listeria monocytogenes using a cdna microarray. upon bacteria challenge, 68 (1.6%) and 112 (2.7%) gene transcripts changed their expression levels ≥2 or ≤2 -fold in gills and digestive tract, respectively. there were 46 tissue-specific transcripts that up-regulated specifically in the digestive ...201121215803
identification, molecular characterization and expression analysis of a mucosal c-type lectin in the eastern oyster, crassostrea virginica.lectins are well known to actively participate in the defense functions of vertebrates and invertebrates where they play an important role in the recognition of foreign particles. they have also been reported to be involved in other processes requiring carbohydrate-lectin interactions such as symbiosis or fertilization. in this study, we report a novel putative c-type lectin (cvml) from the eastern oyster crassostrea virginica and we investigated its involvement in oyster physiology. the cdna of ...201121256965
an mpeg (macrophage expressed gene) from the pacific oyster crassostrea gigas: molecular characterization and gene expression.mpegs (macrophage expressed genes) encode members of the macpf (membrane-attack complex/perforin) protein superfamily that play essential roles in innate immunity. in the present study, a homolog of mpeg1 was identified in crassostrea gigas and designed cg-mpeg1. the complete cdna of cg-mpeg1 is 2781 bp in length, containing an orf of 2226 bp, which encodes a putative protein of 742 amino acids with a predicted 19-aa hydrophobic signal peptide, an macpf domain, and a transmembrane domain. phylog ...201121272653
reverse and flick: hybrid locomotion in bacteria. 201121289282
establishment of a caenorhabditis elegans infection model for vibrio alginolyticus.vibrio alginolyticus, a common bacterium in the marine environment, is a threat to marine animals and humans by causing serious infections. the present study reveals the establishment of a caenorhabditis elegans infection model for vibrio alginolyticus. the infection and colonization was localized in the animal by tagging v. alginolyticus with gfp and using confocal laser scanning microscopy. chemotactic response of c. elegans to v. alginolyticus, pharyngeal distention and blockage of vulval reg ...201121298688
passive immune-protection of small abalone against vibrio alginolyticus infection by anti-vibrio igy-encapsulated feed.small abalone (haliotis diversicolor supertexta) is a high value-added shellfish. it however has been suffering vibrio alginolyticus infections, which cause mass death of small abalone and thus great economic losses, particularly in artificial aquaculture. in this study, we attempted to treat small abalone with anti-vibrio igy to elicit a passive immunity directly against v. alginolyticus infections. anti-vibrio igy was alginate encapsulated in egg powders as feed, which may avoid antibody inact ...201121300158
a selective and differential medium for vibrio this paper, we present a selective and differential medium, termed vibrio alginolyticus (val) agar, developed for the isolation and identification of v. alginolyticus. the presence of bile salts, high salinity and high incubation temperature allows the selective growth of moderately halophilic vibrio species. differentiation of bacteria is achieved by identifying species capable of sucrose fermentation, made visible by the ph indicator bromocresol purple. in this study, all of the 26 strains ...201121306589
enumeration of vibrio parahaemolyticus in the viable but nonculturable state using direct plate counts and recognition of individual gene fluorescence in situ hybridization.vibrio parahaemolyticus is a gram-negative, halophilic bacterium indigenous to marine and estuarine environments and it is capable of causing food and water-borne illness in humans. it can also cause disease in marine animals, including cultured species. currently, culture-based techniques are used for quantification of v. parahaemolyticus in environmental samples; however, these can be misleading as they fail to detect v. parahaemolyticus in a viable but nonculturable (vbnc) state which leads t ...201121329738
susceptibility to antibiotics of vibrio spp. and photobacterium damsela ssp. piscicida strains isolated from italian aquaculture farms.the antibiotic resistance patterns of aetiological agents responsible for vibriosis and pasteurellosis were studied to contribute to control the spread of these two bacterial diseases in mediterranean fish farming. strains of photobacterium damsela ssp. piscicida, vibrio fluvialis, vibrio alginolyticus, vibrio parahaemolyticus, vibrio metschnikovii, isolated from italian aquaculture (fish, shellfish and crustaceans) sites, were assayed for their susceptibility to some antibacterial agents curren ...201121344147
screening and characterization of a cellulase gene from the gut microflora of abalone using metagenomic library.a metagenomic fosmid library was constructed using genomic dna isolated from abalone intestine. screening of a library of 3,840 clones revealed a 36 kb insert of a cellulase positive clone (pamhelo). a shotgun clone library was constructed using the positive clone (pamhelo) and further screening of 3,840 shotgun clones with an approximately 5 kb insert size using a congo red overlay revealed only one cellulase positive clone (pamhl9). the pamhl9 consisted of a 5,293-bp dna sequence and three ope ...201121369991
antimicrobial resistance of vibrio parahaemolyticus and vibrio alginolyticus strains isolated from farmed fish in korea from 2005 through 2007.the antimicrobial resistance patterns to 15 antimicrobial agents of vibrio parahaemolyticus and vibrio alginolyticus isolated from farmed fishes, including olive flounder (paralichthys olivaceus), black rockfish (sebastes schlegeli), red sea bream (pagrus major), and sea bass (lateolabrax japonicus), were investigated from 2005 through 2007. a total of 218 v. parahaemolyticus isolates and 153 v. alginolyticus isolates were obtained from the 180 fish samples collected from fish farms located alon ...201121375873
enhanced immune defences in pacific white shrimp (litopenaeus vannamei) post-exposure to a vibrio vaccine.this study was conducted to determine if exposure of shrimp, litopenaeus vannamei, to a commercial anti-vibrio vaccine caused changes in antibacterial and cellular (phagocytosis) defences. shrimp post-larvae were administered either vibromax™ vaccine or a blank preparation. whole body homogenates were prepared before (day 0), during (day 10) and after (day 20) vaccination and incubated with a selection of pathogenic vibrios. homogenate from day 0 animals showed natural antibacterial activity tow ...201121377471
the cq ratio of surface energy components influences adhesion and removal of fouling bacteria.the interaction energy between bacteria and substrata with different surface energies was modelled by the extended dlvo-á(derjaguin, landau, verwey and overbeek) theory. the modeling results revealed that the interaction energy has a strong correlation with the cq (chen and qi) ratio, which is defined as the ratio of the lifshitz-van der waals (lw) apolar to the electron donor surface energy components of substrata. both modeling and experimental results with different bacteria including p. fluo ...201121390912
genotype analysis of collagenase gene by pcr-sscp in vibrio alginolyticus and its association with virulence to marine this article, the genotypes for vibrio alginolyticus were analyzed based on collagenase gene by pcr-single strand conformation polymorphism (sscp) method and their relationship to the virulence for marine fish were studied by a bacteria challenging experiment. results showed total 13 genotypes designated as a1, a2, a3, a4, b, c, d, e, f, g1, g2, h, and i were identified. among them, isolates from type a1 were dominant in abundance (48.1%) and the isolates from nanyou had the highest polymorph ...201121394507
antimicrobial effects of essential oils of cinnamosma fragrans on the bacterial communities in the rearing water of penaeus monodon larvae.farmed shrimps are vectors of various vibrio species that are considered a potential health hazard. previous study has shown that vibrio parahaemolyticus, vibrio vulnificus, and vibrio alginolyticus dominated in the water and larval samples of shrimp hatchery (randrianarivelo et al. 2010 ). the effects of two essential oils (eos) of cinnamosma fragrans, an endemic plant to madagascar (b8: linalool-type and b143: 1,8-cineole-type), were determined on the total heterotrophic aerobic bacteria and t ...201121395412
sybr green i-based real-time pcr targeting the rpox gene for sensitive and rapid detection of vibrio alginolyticus.rpox, a vibrio alginolyticus specific stress regulating gene, was used to detect this fish pathogen by sybr green i-based real-time pcr. the specificity of the detection was confirmed in different samples. the minimum level of detection was 10(3) cells from pure culture and 10(2) cells from seawater.201121402144
a smaller particle size improved the oral bioavailability of monkey head mushroom, hericium erinaceum, powder resulting in enhancement of the immune response and disease resistance of white shrimp, litopenaeus vannamei.the effects of different particle sizes (100-150, 74-100, and <74 µm) of powder of the dried and ground stipe from the monkey head mushroom, hericium erinaceum, on the immune response and disease resistance of white shrimp, litopenaeus vannamei, against the pathogen, vibrio alginolyticus, were examined. mushroom powder with a particle size of <74 µm had a significantly higher effect on the disease resistance of shrimp compared to particle sizes of >74 µm. mortality of shrimp after being injected ...201121421058
cloning and expression of toll-like receptors 1 and 2 from a teleost fish, the orange-spotted grouper epinephelus coioides.the two toll-like receptors, tlr1 and tlr2 were cloned from orange-spotted grouper, epinephelus coioides, an important teleost fish in mariculture of asia. the cdna sequences of tlr1 and tlr2 are 3195 and 3439 bp long, with an open reading frame (orf) of 2406 and 2466 bp, encoding 801 and 821 amino acids, respectively. the tlr family motifs, i.e. leucine-rich repeat (lrr) domains and toll/interleukin (il)-1 receptor (tir) domains are conserved in the tlr1 and tlr2, with nine and ten leucine-rich ...201121440311
bacterial chemotaxis in an optical optical trapping technique is implemented to investigate the chemotactic behavior of a marine bacterial strain vibrio alginolyticus. the technique takes the advantage that the bacterium has only a single polar flagellum, which can rotate either in the counter-clock-wise or clock-wise direction. the two rotation states of the motor can be readily and instantaneously resolved in the optical trap, allowing the flagellar motor switching rate s(t) to be measured under different chemical stimulatio ...201121494608
a macrophage migration inhibitory factor like oxidoreductase from pearl oyster pinctada fucata involved in innate immune responses.macrophage migration inhibitory factor (mif) is an important cytokine and plays a crucial role as a pivotal regulator of innate immunity. in this study, a mif cdna was identified and characterized from the pearl oyster pinctada fucata (designated as pomif). the full-length of pomif was 1544 bp and consisted of a 5'-untranslated region (utr) of 45 bp, a 3'-utr of 1139 bp with a polyadenylation signal (aataaa) at 12 nucleotides upstream of the poly (a) tail. the open reading frame (orf) of pomif w ...201121496487
cloning of a novel collagenase gene from the gram-negative bacterium grimontia (vibrio) hollisae 1706b and its efficient expression in brevibacillus choshinensis.the collagenase gene was cloned from grimontia (vibrio) hollisae 1706b, and its complete nucleotide sequence was determined. nucleotide sequencing showed that the open reading frame was 2,301 bp in length and encoded an 84-kda protein of 767 amino acid residues. the deduced amino acid sequence contains a putative signal sequence and a zinc metalloprotease consensus sequence, the hexxh motif. g. hollisae collagenase showed 60 and 59% amino acid sequence identities to vibrio parahaemolyticus and v ...201121515782
roles of hfq in the stress adaptation and virulence in fish pathogen vibrio alginolyticus and its potential application as a target for live attenuated vaccine.vibrio alginolyticus has brought about severe economic damage to the mariculture industry by causing vibriosis in various fish species in south china. the virulent determinants of this bacterium have not been well characterized except the exotoxin alkaline serine protease, asp. in addition, the mechanism of virulence regulation in v. alginolyticus remains largely unknown apart from a vibrio harveyi-like quorum sensing (qs) system which is established to manipulate the expression of various virul ...201121523476
transcriptional analysis of disk abalone (haliotis discus discus) antioxidant enzymes against marine bacteria and virus challenge.diverse antioxidant enzymes are essential for marine organisms to overcome oxidative stress as well as for the fine-tuning of immune reactions through activating different signal transduction pathways. this study describes the transcriptional analysis of antioxidant enzymes of disk abalone by challenging with bacteria (vibrio alginolyticus, vibrio parahemolyticus, and listeria monocytogenes) and viral hemorrhagic septicemia virus (vhsv). upon bacteria and vhsv challenge, manganese superoxide dis ...201121549200
cloning and expression of a heat shock protein (hsp) 90 gene in the haemocytes of crassostrea hongkongensis under osmotic stress and bacterial challenge.heat shock protein 90 (hsp90) is a highly conserved and multi-functional molecular chaperone that plays an essential role in both cellular metabolism and stress response. here, we report the cloning of the hsp90 homologue in crassostrea hongkongensis (chhsp90) through ssh in combination with race from cdna of haemocytes. the full-length cdna of chhsp90 is 2459 bp in length, consisting of a 3', 5'-untranslated region (utr) and an open reading frame of 2169 bp encoding 722 amino acids. the identit ...201121565272
heterodisaccharide 4-o-(n-acetyl-β-d-glucosaminyl)-d-glucosamine is an effective chemotactic attractant for vibrio bacteria that produce chitin oligosaccharide deacetylase.aims:  to investigate the attractant effect of 4-o-(n-acetyl-β-d-glucosaminyl)-d-glucosamine (glcnac-glcn) in the chemotaxis of vibrio bacteria that produce carbohydrate esterase (ce) family 4 chitin oligosaccharide deacetylase (cod), an enzyme that catalyzes the production of glcnac-glcn from n,n'-diacetylchitobiose (glcnac)(2) . methods and results:  the chemotactic effect of disaccharides from chitin on several strains of vibrio bacteria was investigated using an agar gel lane-migration metho ...201121575022
mx expression in gilthead sea bream (sparus aurata l.) in response to poly i:c, bacterial lps and chromosomal dna: preliminary study. 201121575726
occurrence of vibrio parahaemolyticus and vibrio alginolyticus in the german bight over a seasonal cycle.bacteria of the genus vibrio are an important component of marine ecosystems worldwide. the genus harbors several human pathogens, for instance the species vibrio parahaemolyticus, a main cause for foodborne gastroenteritis in asia and the usa. pathogenic v. parahaemolyticus strains emerged also in europe, but little is known about the abundance, pathogenicity and ecology of v. parahaemolyticus especially in northern european waters. this study focuses on v. parahaemolyticus and its close relati ...201121598011
characterization of the periplasmic region of pomb, a na+-driven flagellar stator protein in vibrio alginolyticus.the stator proteins poma and pomb form a complex that couples na(+) influx to torque generation in the polar flagellar motor of vibrio alginolyticus. this stator complex is anchored to an appropriate place around the rotor through a putative peptidoglycan-binding (pgb) domain in the periplasmic region of pomb (pomb(c)). to investigate the function of pomb(c), a series of n-terminally truncated and in-frame deletions between the transmembrane (tm) segment and the pgb domain of pomb was constructe ...201121602350
the critical role of a conserved residue, pomb-f22, in the transmembrane segment of the flagellar stator complex for conducting ions and generating torque.bacterial flagellar motors exploit the electrochemical potential gradient of a coupling ion (h(+) or na(+)) as their energy source, and are composed of stator and rotor proteins. sodium-driven and proton-driven motors have the stator proteins, poma and pomb, or mota and motb, respectively, which interact with each other in their transmembrane (tm) regions to form an ion channel. the single tm region of pomb or motb, which forms the ion-conduction pathway together with tm3 and tm4 of poma or mota ...201121636648
the structure of the flagellar motor protein complex pomab: implications for the torque-generating conformation.the bacterial flagellar motor is driven by an ion flux through a channel called motab in escherichia coli or salmonella and pomab in vibrio alginolyticus. pomab is composed of two transmembrane (tm) components, poma and pomb, and converts a sodium ion flux to rotation of the flagellum. its homolog, motab, utilizes protons instead of sodium ions. pomb/motb has a peptidoglycan (pg)-binding motif in the periplasmic domain, allowing it to function as the stator by being anchored to the pg layer. to ...201121642461
white shrimp litopenaeus vannamei immersed in seawater containing sargassum hemiphyllum var. chinense powder and its extract showed increased immunity and resistance against vibrio alginolyticus and white spot syndrome virus.this study was to examine the immune response of white shrimp litopenaeus vannamei and its resistance against vibrio alginolyticus and wssv when shrimp received the sargassum hemiphyllum var. chinense powder and its hot-water extract. both powder and extract showed activation of prophenoloxidase and generation of superoxide anion in the shrimp in vitro. the haemocyte count, phenoloxidase (po) activity, respiratory burst, and lysozyme activity were examined after the shrimp were immersed in seawa ...201121658452
anticoagulant, antiherpetic and antibacterial activities of sulphated polysaccharide from indian medicinal plant tridax procumbens l. (asteraceae).the sulphated polysaccharide from the widespread tridax procumbens plant was studied for the anticoagulant, antiherpetic and antibacterial activity. the anticoagulant activity was determined by the activated partial thromboplastin time assay. the sulphated polysaccharide from t. procumbens represented potent anticoagulant reaching the efficacy to heparin and chondroitin sulphate. moreover, the sulphated polysaccharide extracted from t. procumbens was found non-toxic on vero cell lines up to the ...201121671053
ktrb, a member of the superfamily of k+ transporters.ktrb is the k(+)-translocating subunit of the k(+)-uptake system ktrab from bacteria. it is a member of the superfamily of k(+)transporters (skt proteins) with other sub-families occurring in archaea, bacteria, fungi, plants and trypanosomes. skt proteins may have originated from small k(+) channels by at least two gene duplication and two gene fusion events. they contain four covalently linked m(1)pm(2) domains, in which m(1) and m(2) stand for transmembrane stretches, and p for a p-loop, which ...201121680052
Molecular cloning, characterization and expression analysis of the tumor necrosis factor (TNF) superfamily gene, TNF receptor superfamily gene and lipopolysaccharide-induced TNF-+¦ factor (LITAF) gene from Litopenaeus vannamei.In vertebrates, the tumor necrosis factor (TNF)-receptor (TNFR) system participates in diverse physiological and pathological events, such as inflammation and protective immune responses to microbial infections. There are few reports about the role of the invertebrate TNF-TNFR system in immune responses. Here, we isolated and characterized the TNF superfamily (LvTNFSF) gene, TNFR superfamily (LvTNFRSF) gene and lipopolysaccharide-induced TNF-+¦ factor (LvLITAF) gene from Litopenaeus vannamei. Lv ...201121736897
two superoxide dismutase (sod) with different subcellular localizations involved in innate immunity in crassostrea hongkongensis.sods are ubiquitous metalloenzymes that can scavenge superoxides in response to various stresses. in the present study, full-length cdnas of two sod genes were isolated from crassostrea hongkongensis (designated chmnsod and chcuznsod). the cdnas are 997 and 918 bp in length with orfs of 675 and 468 bp (encoding 225 and 156 amino acids), respectively. sequence analysis revealed a conserved sod_fe domain in chmnsod, and a sod_cu_zn domain in chcuznsod. subcellular localization of chmnsod is mitoch ...201121745578
gene expression analysis of clams ruditapes philippinarum and ruditapes decussatus following bacterial infection yields molecular insights into pathogen resistance and immunity.the carpet shell clam (ruditapes decussatus) and manila clam (ruditapes philippinarum), which are cultured bivalve species with important commercial value, are affected by diseases that result in large economic losses. because the molecular mechanism of the immune response of bivalves, especially clams, is scarce and fragmentary, we have examined all expressed sequence tags (est) resources available in public databases for these two species in order to increase our knowledge on genes related wit ...201121756933
molecular cloning and expression analysis of heat-shock protein 70 in orange-spotted grouper epinephelus coioides following heat shock and vibrio alginolyticus this study, the complementary (c)dna encoding heat-shock protein 70 (hsp70) of orange-spotted grouper epinephelus coioides (osghsp70) was cloned. osghsp70 was 2206 bp and encoded 652 amino acids with predicted molecular mass of 70·89 kda and theoretical isoelectric point of 5·48. three hsp70 family signatures, bipartite nuclear localization signal sequence (nls) and cytoplasmic characteristic motif (eevd) were observed in the osghsp70, which shared high similarity in amino-acid sequences with ...201121781104
kalopanax pictus as feed additive controls bacterial and parasitic infections in kelp grouper, epinephelus bruneus.feeding kelp grouper, epinephelus bruneus (26.1 ± 1.4), with 0%, 0.1%, 1.0%, and 2.0% kalopanax pictus extract-supplementation diets, for 30 days reduced mortality on being challenged intraperitoneally 100 µl with bacterium vibrio alginolyticus (2.1 × 10(7) cfu ml(-1)) and ciliate parasite philasterides dicentrarchi (2.3 × 10(7) ciliates ml(-1)). the red blood cells (rbc), white blood cells (wbc), haemoglobin, haematocrit, lymphocytes, and monocytes levels significantly increased in kelp grouper ...201121782952
cloning and expression of an actin gene in the haemocytes of pearl oyster (pinctada fucata, gould 1850).an actin gene (designated pfact1) of pearl oyster, pinctada fucata, was cloned from haemocytes by the techniques of homological cloning and rapid amplification of cdna ends (race). the full length of pfact1 cdna was 1608-ábp in length, having a 5' untranslated region (utr) of 82-ábp, a 3' utr of 395-ábp, and an open reading frame (orf) of 1131-ábp encoding a polypeptide of 376 amino acids with a predicted molecular weight of 41.76-ákda and an estimated isoelectric point of 5.29. sequence analysi ...200821798155
dietary administration of a gracilaria tenuistipitata extract enhances the immune response and resistance against vibrio alginolyticus and white spot syndrome virus in the white shrimp litopenaeus vannamei.the haemogram, phenoloxidase (po) activity, respiratory bursts (rbs), superoxide dismutase (sod) activity, glutathione peroxidase (gpx) activity, lysozyme activity, and the mitotic index of haematopoietic tissue (hpt) were examined after the white shrimp litopenaeus vannamei had been fed diets containing the hot-water extract of gracilaria tenuistipitata at 0 (control), 0.5, 1.0, and 2.0-ág-ákg(-1) for 7-35-ádays. results indicated that these parameters directly increased with the amount of extr ...201121820059
molecular cloning, characterization and expression analysis of two novel tolls (lvtoll2 and lvtoll3) and three putative sp+ñtzle-like toll ligands (lvspz1-3) from litopenaeus vannamei.toll-like receptor-mediated nf-+¦b pathways are essential for inducing immune related-gene expression in the defense against bacterial, fungal and viral infections in insects and mammals. although a toll receptor (lvtoll1) was cloned in litopenaeus vannamei, relatively little is known about other types of toll-like receptors and their endogenous cytokine-like ligand, sp+ñtzle. here, we report two novel toll-like receptors (lvtoll2 and lvtoll3) and three sp+ñtzle-like proteins (lvspz1-3) from l. ...201121827783
salt gland adenitis as only cause of stranding of loggerhead sea turtles caretta caretta.the present study describes pathological and microbiological findings in 9 stranded loggerhead sea turtles caretta caretta, whose only observed lesion was bilateral purulent salt gland adenitis. histological lesions ranged from the presence of abundant eosinophilic material associated with bacterial colonies in the lumen of the central ducts of the glandular lobules to the destruction of the glandular tissue and presence of abundant eosinophilic material composed of heterophils and cell debris, ...201121848124
a tandem-repeat galectin involved in innate immune response of the pearl oyster pinctada fucata.the cdna of a tandem-repeat galectin from the pearl oyster pinctada fucata (designated pfgal) was cloned by expressed sequence tag (est) and rapid amplification of cdna ends (race) techniques. the full-length cdna of pfgal was 1386bp, consisting of a 5' untranslated region (utr) of 26bp, a 3' utr of 313bp, and an open reading frame (orf) of 1047bp encoding a polypeptide of 348 amino acids with a predicted molecular weight of 38.09kda and theoretical isoelectric point of 8.49. similar to other ta ...201121867976
sodium-driven motor of the polar flagellum in marine bacteria vibrio.the na(+) -driven bacterial flagellar motor is a molecular machine powered by an electrochemical potential gradient of sodium ions across the cytoplasmic membrane. the marine bacterium vibrio alginolyticus has a single polar flagellum that enables it to swim in liquid. the flagellar motor contains a basal body and a stator complexes, which are composed of several proteins. poma, pomb, motx, and moty are thought to be essential components of the stator that are required to generate the torque of ...201121895888
biological characterization of two marine bdellovibrio-and-like organisms isolated from daya bay of shenzhen, china and their application in the elimination of vibrio parahaemolyticus in oyster.bdellovibrio-and-like organisms (balos) are a group of highly motile delta-proteobacteria that prey on other gram-negative bacteria. however, nothing is known of the application potential of marine balos in safeguarding seafood safety. here, biological characterization of two marine balos strains and their application in the elimination of vibrio parahaemolyticus in oyster (crassostrea ariakensis) at the laboratory scale were investigated. balos strains bdh12 and bdhsh06 were isolated from sedim ...201121899909
[preparation and identification of ompw monoclonal antibodies].to prepare and characterize the mouse monoclonal antibodies against vibrio parahaemolyticus ompw.201121906472
a novel sialic acid binding lectin with anti-bacterial activity from the hong kong oyster (crassostrea hongkongensis).lectins play an important role in immune recognition and host defense. in the present study, a full-length cdna encoding a novel sialic acid binding lectin was cloned from crassostrea hongkongensis (designated ch-salectin) by rapid amplification of cdna ends (race). it is 531 bp in length, containing a 21 bp 5' utr, a 39 bp 3' utr and a 468 bp orf coding for 156 amino acids. the ch-salectin protein contains a signal peptide and a conserved complement component c1q domain. the purified recombinan ...201121906682
characterization of role of the toxr gene in the physiology and pathogenicity of vibrio alginolyticus.toxr, a conserved virulence-associated gene in vibrios, is identified in vibrio alginolyticus zj51-o, a pathogenic strain isolated from diseased fish. to reveal the role of toxr in the pathogenicity of v. alginolyticus, a deletion mutant was constructed by allelic exchange. the mutant showed the same level of growth in trypticase soy broth (tsb) and iron-limiting condition, as the wild type strain. however, deletion of toxr severely reduced resistance against bile salts and the capability of bio ...201121915613
cdna cloning and mrna expression of a tandem-repeat galectin (pogal2) from the pearl oyster, pinctada fucata.galectins can recognize and specifically bind to β-galactoside residues, playing crucial roles in innate immune responses of vertebrates and invertebrates. we cloned the cdna of a tandem-repeat galectin from the pearl oyster pinctada fucata (designated as pogal2). pogal2 cdna is 1347 bp long and consists of a 5'-untranslated region (utr) of 3 bp, a 3'-utr of 297 bp with one cytokine rna instability motif (attta), and an open reading frame of 1047 bp, encoding a polypeptide of 349 amino acids, wi ...201121948759
a putative g protein-coupled receptor involved in innate immune defense of procambarus clarkii against bacterial infection.the immune functions of g protein-coupled receptor (gpcr) were widely investigated in mammals. however, limited researches on immune function of gpcrs were reported in invertebrates. in the present study, the immune functions of hp1r gene, a putative gpcr identified from red swamp crayfish procambarus clarkii were reported. expression of hp1r gene was significant up-regulated in response to heat-killed aeromonas hydrophila challenge. hp1r gene silencing mediated by rna interference significantly ...201121964155
Vibrio jasicida sp. nov., a member of the Harveyi clade, from marine animals (packhorse lobster, abalone, and Atlantic salmon).Six isolates of a facultatively anaerobic bacterium were recovered in culture from marine invertebrates and vertebrates, including packhorse lobster (Jasus verreauxi), abalone (Haliotis sp.) and Atlantic salmon (Salmo salar) between 1994 and 2002. The bacteria are Gram-negative, rod shaped and motile by means of more than one polar flagellum, oxidase-positive, catalase-positive and able to grow between 0.5-8.0% NaCl (optimum 3.0-6.0%) and at temperatures of 10-37 °C (optimum 25-30 °C). On the ba ...201121984666
Mutations targeting the C-terminal domain of FliG can disrupt motor assembly in the Na(+)-driven flagella of Vibrio alginolyticus.The torque of the bacterial flagellar motor is generated by the rotor-stator interaction coupled with specific ion translocation through the stator channel. To produce a fully functional motor, multiple stator units must be properly incorporated around the rotor by an as yet unknown mechanism to engage the rotor-stator interactions. Here, we investigated stator assembly using a mutational approach of the Na(+)-driven polar flagellar motor of Vibrio alginolyticus, whose stator is localized at the ...201121986199
occurance and survival of vibrio alginolyticus in tamouda bay (morocco).the objectives of this study were to investigate the spatial and seasonal fluctuations of vibrio alginolyticus in marine environment of the tamouda bay on the mediterranean coast of morocco and to determine the dominant factors of the environment that govern these fluctuations. the samples (sea water, plankton, shellfish and sediment) were collected fortnightly for two years from three study sites on the coast tamouda bay in northern morocco. the charge of vibrio alginolyticus is determined by m ...201122000489
Secreted glyceraldehyde-3-phosphate dehydrogenase as a broad spectrum vaccine candidate against microbial infection in aquaculture.Aims: Subcellullar localizations and cross-immunities of GAPDHs from six common pathogenic bacteria in aquaculture were investigated. Methods and Results: Subcellullar localizations of GAPDHs of Edwardsiella tarda EIB202, Edwardsiella ictaluri ATCC33202, Aeromonas hydrophila LSA34, Vibrio anguillarum MVM425, Vibrio alginolyticus EPGS020401 and Vibrio harveyi VIB647 were analysed with Western blotting, indirect immunofluorescence and flow cytometry examinations. Immunoprotections of different r ...201222017642
vibrio alginolyticus-associated wound infection acquired in british waters, guernsey, july july 2011, a vibrio alginolyticus infection was diagnosed in a woman from guernsey in the channel islands, british isles after sea bathing and application of a natural seaweed dressing to a pre-existing leg wound. microbiological investigation confirmed vibrio in the wound and the species of seaweed used for the dressing. the washing of open wounds in seawater and use of unsterilised seaweed dressings should be discouraged, particularly in individuals with underlying risk conditions.201122027377
Susceptibility of a Vibrio alginolyticus rpoS mutant to environmental stresses and its expression of OMPs.Vibrio alginolyticus, one of the most important opportunistic pathogens, can be detected in human being and marine animals. Like other bacteria, V. alginolyticus is able to adapt to a variety of stressful environmental changes. The alternate sigma factor RpoS, which is a regulator during stationary phase, plays an important role in surviving under these stressful situations in many bacteria. Sequence analysis reveals a 990 bp open reading frame which is predicted to encode a 330-amino-acid prote ...201122052546
Characterization of anticancer, antimicrobial, antioxidant properties and chemical compositions of Peperomia pellucida leaf extract.Peperomia pellucida leaf extract was characterized for its anticancer, antimicrobial, antioxidant activities, and chemical compositions. Anticancer activity of P. pellucida leaf extract was determined through Colorimetric MTT (tetrazolium) assay against human breast adenocarcinoma (MCF-7) cell line and the antimicrobial property of the plant extract was revealed by using two-fold broth micro-dilution method against 10 bacterial isolates. Antioxidant activity of the plant extract was then charact ...201122071643
In vitro evaluation of antibacterial activity of Asparagopsis taxiformis from the Straits of Messina against pathogens relevant in aquaculture.Ethanol extracts of Asparagopsis taxiformis collected from the Straits of Messina (Italy) were screened for antibacterial activity against pathogenic shellfish and fish bacteria previously isolated from local marine and brackish environments. Genetic labelling by DNA barcoding allowed us to identify the algal population as a biogeographical strain conspecific to A. taxiformis. The extract obtained in May showed the broadest antibacterial activity against all tested pathogenic bacteria, especiall ...201222078751
Cloning and expression of arginine kinase from a swimming crab, Portunus trituberculatus.Arginine kinase (AK) is an important phosphotransferase that plays a critical role in energy metabolism in invertebrates. In this paper, the cDNA of AK (designated as PtAK) was identified from the eyestalk cDNA library of swimming crab Portunus trituberculatus. The full-length cDNA was 1,479 bp, containing an open reading frame of 1,074 bp that coded for 357 amino acids. The estimated molecular mass of mature PtAK was 40.30 kDa and theoretical isoelectric point was 6.18. Amino acid sequence alig ...201122101972
molecular characterization and mrna expression of catalase from pearl oyster pinctada fucata.catalase (ec is an important antioxidant enzyme that protects aerobic organisms against oxidative damage by degrading hydrogen peroxide to water and oxygen. in the present study, a catalase cdna of peal oyster pincatada fucata (designated as pocat) is cloned and characterized by expressed sequence tag (est) and rapid amplification of cdna ends (race) methods. pocat is 2428 bp long and consists of a 5'-utr of 140 bp, an unusually long 3'-utr of 749 bp, and an open reading frame (orf) of ...201122118636
Phenoloxidase in the scallop Chlamys farreri: Purification and antibacterial activity of its reaction products generated in vitro.Phenoloxidase (PO) was purified from hemocytes of the scallop Chlamys farreri using native-PAGE and gel permeation column chromatography, and then substrate specificity and antibacterial activity generated from reaction products of purified PO were analyzed. The results showed purified PO had a molecular mass of 576 kDa in native-PAGE and 53 kDa in denatured PAGE, and could catalyze the substrates L-3,4-dihydroxyphenylalanine (L-DOPA), dopamine, catechol and hydroquinone suggesting it is a type ...201222119576
characterization of a new quorum sensing regulator luxt and its roles in the extracellular protease production, motility, and virulence in fish pathogen vibrio alginolyticus.vibrio alginolyticus, an opportunistic pathogen that causes vibriosis in miscellaneous fish species, has brought about serious economic damage to the mariculture industry in south china. the mechanism of virulence regulation in v. alginolyticus is yet not known except a vibrio harveyi-like quorum sensing (qs) system that is established to manipulate the expression of diverse genes including those encoding virulence determinants. in this study, a new tetr family qs regulator, luxt, was identified ...201122130678
The first homolog of pacifastin-related precursor in the swimming crab (Portunus trituberculatus): Characterization and potential role in immune response to bacteria and fungi.Among all the serine proteinase inhibitor families (SPIs), the pacifastin-related inhibitor is seldom isolated. A pacifastin-related SPI named as PtPLC was identified from the swimming crab Portunus trituberculatus in this study. The full-length of PtPLC was cloned from haemocytes cDNA library by the combination of homology cloning and rapid amplification of cDNA ends (RACE). The PtPLC contained an open reading frame (ORF) of 1098 bp encoding a putative pacifastin-related precursor of 365 amino ...201222154999
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