PMID(sorted ascending)
noradrenaline modulates the immunity of white shrimp litopenaeus vannamei.the total haemocyte count (thc), phenoloxidase activity, respiratory burst, superoxide dismutase (sod) activity, phagocytic activity and clearance efficiency in response to pathogen vibrio alginolyticus were measured when the white shrimp litopenaeus vannamei (18.4 +/- 1.2 g) were injected individually with noradrenaline at 10(-8), 10(-7) and 10(-6) mol shrimp(-1). for the shrimp that received noradrenaline at 10(-8), 10(-7) and 10(-6) mol shrimp(-1), the thc decreased by 15%, 21% and 32%, pheno ...200616377211
electron cryomicroscopic visualization of poma/b stator units of the sodium-driven flagellar motor in liposomes.a motor protein complex of the bacterial flagellum, poma/b from vibrio alginolyticus, was reconstituted into liposomes and visualized by electron cryomicroscopy. poma/b is a sodium channel, composed of two membrane proteins, poma and pomb, and converts ion flux to the rotation of the flagellar motor. escherichia coli and salmonella have a homolog called mota/b, which utilizes proton instead of sodium ion. pomb and motb have a peptidoglycan-binding motif in their c-terminal region, and therefore ...200616426637
regulation of polar flagellar number by the flhf and flhg genes in vibrio alginolyticus.the number and location of bacterial flagella vary with the species. the vibrio alginolyticus cell has a single polar flagellum, which is driven by sodium ions. we selected mutants on the basis of reduced swarming ability on soft agar plates. among them, we found two mutants with multiple polar flagella, and named them kk148 and nmb155. in pseudomonas species, it is known that flhf and flen, which are ftsy and mind homologs, respectively, are involved in regulation of flagellar placement and num ...200616428326
roles of charged residues of rotor and stator in flagellar rotation: comparative study using h+-driven and na+-driven motors in escherichia escherichia coli, rotation of the flagellar motor has been shown to depend upon electrostatic interactions between charged residues of the stator protein mota and the rotor protein flig. these charged residues are conserved in the na+-driven polar flagellum of vibrio alginolyticus, but mutational studies in v. alginolyticus suggested that they are relatively unimportant for motor rotation. the electrostatic interactions detected in e. coli therefore might not be a general feature of flagellar ...200616452430
a marine bacterium, micrococcus mccb 104, antagonistic to vibrios in prawn larval rearing systems.a marine bacterium, micrococcus mccb 104, isolated from hatchery water, demonstrated extracellular antagonistic properties against vibrio alginolyticus, v. parahaemolyticus, v. vulnificus, v. fluviallis, v. nereis, v. proteolyticus, v. mediterranei, v cholerae and aeromonas sp., bacteria associated with macrobrachium rosenbergii larval rearing systems. the isolate inhibited the growth of v. alginolyticus during co-culture. the antagonistic component of the extracellular product was heat-stable a ...200516465832
cloning and expression analysis of interferon-gamma-inducible-lysosomal thiol reductase gene in large yellow croaker (pseudosciaena crocea).in mammals, interferon-gamma-inducible-lysosomal thiol reductase (gilt) has been demonstrated to play a key role in the processing and presentation of mhc class ii-restricted antigen (ag) by catalyzing disulfide bond reduction, thus unfolding native protein ag and facilitating subsequent cleavage by proteases. here, we reported the cloning of a gilt gene homologue from the spleen of large yellow croaker, a marine fish (lycgilt). the full-length cdna of lycgilt gene is 1033 nucleotides (nt) encod ...200616478632
crystallization of the nadh-oxidizing domain of the na+-translocating nadh:ubiquinone oxidoreductase from vibrio cholerae.the na+-translocating nadh:quinone oxidoreductase (na+-nqr) from pathogenic and marine bacteria is a respiratory complex that couples the exergonic oxidation of nadh by quinone to the transport of na+ across the membrane. the nqrf subunit oxidizes nadh and transfers the electrons to other redox cofactors in the enzyme. the fad-containing domain of nqrf has been expressed, purified and crystallized. the purified nqrf fad domain exhibited high rates of nadh oxidation and contained stoichiometric a ...200616511277
molecular cloning of growth hormone from silver sea bream: effects of abiotic and biotic stress on transcriptional and translational expression.the pituitary growth hormone (gh) gene of silver sea bream (sparus sarba) was cloned and characterized and found to be 615 base pairs encoding a protein of 204 amino acids. using a bacterial expression system, recombinant protein was prepared and rabbit polyclonal antibody was raised. transcript and protein amounts of gh were measured in fish that were adapted to a range of salinities, acclimated to different temperatures, or undergoing a natural time course of vibrio alginolyticus infection. is ...200616513086
use of multiparameter analysis for vibrio alginolyticus viable but nonculturable state determination.vibrio alginolyticus is known to enter into a viable but nonculturable (vbnc) state in response to environmental conditions unfavorable to the growth. cells in vbnc condition pose a public health threat because they are potentially pathogenic.200616528721
isolation of a pathogenic strain of vibrio alginolyticus from necrotic larvae of macrobrachium rosenbergii (de man). 200616533305
comparison of the growth and survival of larval turbot in the absence of culturable bacteria with those in the presence of vibrio anguillarum, vibrio alginolyticus, or a marine aeromonas sp.larval turbot (scophthalmus maximus) were reared on rotifers (brachionus plicatilis) in the absence of culturable bacteria for up to 14 days and exhibited growth and high rates of survival (>55% in five experiments). low numbers of known bacteria were introduced into similar cultures by exposure of the rotifers to a suspension of bacteria prior to addition of rotifers to the larval cultures; vibrio anguillarum 91079 caused a highly significant decrease (p <0.01) in the proportion of survivors in ...199516535196
affinity proteomic approach for identification of an iga-like protein in litopenaeus vannamei and study on its agglutination unknown protein reacted with anti-human iga, namely, iga-like protein, has been reported in shrimp, but information regarding its identification is not available. in the present study, an affinity proteomic strategy was applied to identify the iga-like protein of shrimp litopenaeus vannamei. the protein of 75 kda was isolated and confirmed by affinity chromatography and western blotting with goat anti-human iga, respectively, and then identified as hemocyanin, a member of igsf, by mass spectr ...200616602688
effect of sulfide on the immune response and susceptibility to vibrio alginolyticus in the kuruma shrimp marsupenaeus japonicus.kuruma shrimp marsupenaeus japonicus held in 34 per thousand seawater were injected with tryptic soy broth (tsb)-grown vibrio alginolyticus (2.7x10(6)cfu shrimp(-1)), and then placed in water containing concentrations of sulfide at 0 (control), 51, 106, 528 and 1050microgl(-1), respectively. after 12-144h, mortality of v. alginolyticus-injected shrimp exposed to 528 and 1102microgl(-1) sulfide was significantly higher than that of shrimp exposed to 51microgl(-1) sulfide and the control solution. ...200716713303
a function of polar flagellum and anisotropic growth in vibrio alginolyticus early-phase colonies.we continuously observed growth of vibrio alginolyticus early-phase colonies on agar plates by phase-contrast microscopy. two mutants defective in motility on solid surfaces were used in this study: one (ym4) can swim in liquid environments using its polar flagellum, and the other (nmb198) cannot swim because it lacks any flagella. we found that isolated colonies of ym4 were generally more circular than those of nmb198. this observation suggests that ym4 cells moved slightly within a colony by t ...200616732454
the immunostimulatory effects of sodium alginate and iota-carrageenan on orange-spotted grouper epinephelus coicoides and its resistance against vibrio alginolyticus.the lysozyme activity, alternative complement activity (ach50), respiratory burst, sod (superoxide dismutase) activity and phagocytic activity of orange-spotted grouper epinephelus coicoides were examined when the fish were injected intraperitoneally with sodium alginate at 10, 20, 30mgkg-1 and iota-carrageenan at 10, 20, 30mgkg-1, respectively after 24, 72 and 120 h. serum ach50 increased directly with dose after 24 and 72 h for both sodium alginate and iota-carrageenan treatments. the fish tha ...200716784873
soft-agar-coated filter method for early detection of viable and thermostable direct hemolysin (tdh)- or tdh-related hemolysin-producing vibrio parahaemolyticus in seafood.a novel method for detecting viable and thermostable direct hemolysin (tdh)-producing or tdh-related hemolysin (trh)-producing vibrio parahaemolyticus in seafood was developed. the method involved (i) enrichment culture, selective for viable, motile cells penetrating a soft-agar-coated filter paper, and (ii) a multiplex pcr assay targeting both the tdh gene (tdh) and trh gene (trh) following dnase pretreatment on the test culture to eradicate any incidental dnas that might have been released fro ...200616820446
low flagellar motor torque and high swimming efficiency of caulobacter crescentus swarmer cells.we determined the torque of the flagellar motor of caulobacter crescentus for different motor rotation rates by measuring the rotation rate and swimming speed of the cell body and found it to be remarkably different from that of other bacteria, such as escherichia coli and vibrio alginolyticus. the average stall torque of the caulobacter flagellar motor was approximately 350 pn nm, much smaller than the values of the other bacteria measured. furthermore, the torque of the motor remained constant ...200616844761
virulence and enterobacterial repetitive intergenic consensus pcr of vibrio alginolyticus strains isolated from tunisian cultured gilthead sea bream and sea bass outbreaks.vibrio alginolyticus was isolated from the internal organs of diseased gilthead sea bream (sparus aurata) and sea bass (dicentrarchus labrax) cultured in two fish farms located on the tunisian mediterranean coast, from 2003 to 2005. after phenotypic characterisation, a selection of 34 isolates from gilthead sea bream and sea bass were molecularly typed by repetitive intergenic consensus pcr (eric-pcr) showing a high polymorphism among the isolated strains (19 genotypes). most of the isolates wer ...200616870360
salmonella spp., vibrio spp., clostridium perfringens, and plesiomonas shigelloides in marine and freshwater invertebrates from coastal california ecosystems.the coastal ecosystems of california are highly utilized by humans and animals, but the ecology of fecal bacteria at the land-sea interface is not well understood. this study evaluated the distribution of potentially pathogenic bacteria in invertebrates from linked marine, estuarine, and freshwater ecosystems in central california. a variety of filter-feeding clams, mussels, worms, and crab tissues were selectively cultured for salmonella spp., campylobacter spp., escherichia coli-o157, clostrid ...200616897302
development of monoclonal antibodies for simple identification of vibrio alginolyticus.the present study was aimed to produce monoclonal antibodies (mabs) for simple and specific identification of vibrio alginolyticus infection in shrimp.200616965376
the vibrio motor proteins, motx and moty, are associated with the basal body of na-driven flagella and required for stator formation.the four motor proteins poma, pomb, motx and moty, which are believed to be stator proteins, are essential for motility by the na(+)-driven flagella of vibrio alginolyticus. when we purified the flagellar basal bodies, motx and moty were detected in the basal body, which is the supramolecular complex comprised of the rotor and the bushing, but poma and pomb were not. by antibody labelling, motx and moty were detected around the lp ring. these results indicate that motx and moty associate with th ...200617038120
characterization of a vibrio alginolyticus strain, isolated from alaskan oysters, carrying a hemolysin gene similar to the thermostable direct hemolysin-related hemolysin gene (trh) of vibrio parahaemolyticus.a vibrio strain isolated from alaskan oysters and classified by its biochemical characteristics as vibrio alginolyticus possessed a thermostable direct hemolysin-related hemolysin (trh) gene previously reported only in vibrio parahaemolyticus. this trh-like gene was cloned and sequenced and was 98% identical to the trh2 gene of v. parahaemolyticus. this gene seems to be functional since it was transcriptionally active in early-stationary-phase growing cells. to our knowledge, this is the first r ...200617056701
aerobic microorganisms associated with free-ranging bottlenose dolphins in coastal gulf of mexico and atlantic ocean waters.our abilities to assess health risks to free-ranging dolphin populations, to treat live-stranded or captive dolphins, and to evaluate the risks of disease transmission between humans and dolphins have suffered from a lack of basic information on microorganisms associated with normal, presumably healthy free-ranging individuals. in order to provide these data, we sampled free-ranging bottlenose dolphins (tursiops truncatus) off florida, texas, and north carolina during 1990-2002. blowhole and ana ...200617092884
genomic plasticity of the rrn-nqrf intergenic segment in the chlamydiaceae, the nucleotide sequence between the 5s rrna gene and the gene for subunit f of the na(+)-translocating nadh-quinone reductase (nqrf or dmpp) has varied lengths and gene contents. we analyzed this site in 45 chlamydiaceae strains having diverse geographical and pathological origins and including members of all nine species.200717158668
rapid detection of vibrio species using liquid microsphere arrays and real-time pcr targeting the ftsz locus.the development of rapid and sensitive molecular techniques for the detection of vibrio species would be useful for the surveillance of sporadic infections and management of major outbreaks. comparative sequence analysis of the ftsz gene in the predominant vibrio species that cause human disease revealed distinct alleles for each examined species, including vibrio cholerae, vibrio parahaemolyticus and vibrio vulnificus. light upon extension (lux) real-time pcr assays were developed to target the ...200717172518
the immunostimulatory effects of hot-water extract of gelidium amansii via immersion, injection and dietary administrations on white shrimp litopenaeus vannamei and its resistance against vibrio alginolyticus.the total haemocyte count (thc), phenoloxidase activity, and respiratory burst were examined when white shrimp litopenaeus vannamei were immersed in seawater (34 per thousand) containing hot-water extract of red alga gelidium amansii at 200, 400 and 600 mg l(-1), injected with hot-water extract at 4 and 6 microg g(-1) shrimp, and fed diets containing hot-water extract at 0, 0.5, 1.0 and 2.0 g kg(-1). these parameters increased significantly when shrimp were immersed in seawater containing hot-wa ...200717194604
identification and cloning of the antioxidant enzyme, glutathione peroxidase, of white shrimp, litopenaeus vannamei, and its expression following vibrio alginolyticus infection.cdna encoding glutathione peroxidase (gpx) mrna of the white shrimp litopenaeus vannamei was obtained from haemocytes by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna (race) using oligonucleotide primers based on the gpx sequence of homo sapiens (nm002083), mus musculus (nm008160), arabidopsis thaliana (u94495), bos taurus (nm174770), and capsicum chinense (aj973135). the 727-bp cdna contained an open reading frame (orf) of 567 bp, a 101-bp 5'-untrans ...200717208011
hedistin: a novel antimicrobial peptide containing bromotryptophan constitutively expressed in the nk cells-like of the marine annelid, nereis diversicolor.a novel antimicrobial peptide, named hedistin was identified from the coelomocytes of nereis diversicolor. hedistin shows no obvious similarities with other known peptides and constitutes the first antimicrobial peptide containing bromotryptophans demonstrated in annelids. cdna and mass spectrometry analysis revealed that, upon bacteria challenge, this peptide is secreted following processing of a precursor containing a signal peptide and prosequences. hedistin was shown to possess an activity a ...200717210178
vibrio alginolyticus infections in the netherlands after swimming in the north sea. 200617213549
crystallization and preliminary x-ray analysis of moty, a stator component of the vibrio alginolyticus polar flagellar motor.the polar flagellum of vibrio alginolyticus is rotated by the sodium motor. the stator unit of the sodium motor consists of four different proteins: poma, pomb, motx and moty. motx and moty, which are unique components of the sodium motor, form the t-ring structure attached to the lp ring in the periplasmic space. moty has a putative peptidoglycan-binding motif in its c-terminal region and motx is suggested to interact with pomb. thus, motx and moty are thought to be required for incorporation a ...200717277446
the effect of dietary chitin supplementation on the survival and immune reactivity of the shore crab, carcinus male shore crabs (carcinus maenas) were maintained on a fish-based diet supplemented with 0, 5 or 10% chitin for 11 weeks. significantly greater mortality was found during this period in the control group (0% chitin) than those fed 10% chitin. crabs fed 5 or 10% chitin had lower numbers of cultivatable bacteria in the hepatopancreas than those on the basal diet alone. the addition of chitin had no significant effect on the serum concentrations of protein and glucose, and the levels of glyc ...200717289410
occurrence of vibrio spp. in blue mussels (mytilus edulis) from the german wadden sea.the present study aimed at determining the contamination of blue mussels grown in germany with relevant bacterial organisms. seasonal variations were examined. for that purpose, mussel samples were taken regularly between june 2004 and may 2005 in seven shellfish-growing areas of the german wadden sea. a total of 90 samples were analysed. the analysis included the compulsory microbiological parameters escherichia coli and salmonella spp., as well as the determination of the contamination with vi ...200717331605
immune responses and gene expression in white shrimp, litopenaeus vannamei, induced by lactobacillus plantarum.the total haemocyte counts, phenoloxidase (po) activity, respiratory bursts, superoxide dismutase (sod) activity, and phagocytic activity and clearance efficiency to vibrio alginolyticus, as well as prophenoloxidase (propo), lipopolysaccharide- and beta-1,3-glucan-binding protein (lgbp), serine protein (sp), and peroxinectin (pe) mrna transcription of l. vannamei, and its susceptibility to v. alginolyticus when the shrimp were fed diets containing lactobacillus plantarum at 0 (control), 10(7), a ...200717337209
expression, characterization and immunogenicity of a major outer membrane protein from vibrio alginolyticus.vibrio alginolyticus is one of the vibrio pathogens common to humans and marine animals. during infection and induction of the host immune response, outer membrane proteins of bacteria play an important role. in this study, an outer membrane protein gene (ompw) was cloned from v. alginolyticus and expressed in escherichia coli. the 645 bp open reading frame (orf) encodes a protein of 214 amino acid residues with a predicted molecular weight of 23.3 kda. the amino acid sequence showed a high iden ...200717342258
atp binding to the ktn/rck subunit ktra from the k+ -uptake system ktrab of vibrio alginolyticus: its role in the formation of the ktrab complex and its requirement in vivo.subunit ktra of the bacterial na(+)-dependent k(+)-translocating ktrab systems belongs to the ktn/rck family of regulatory proteins and protein domains. they are located at the cytoplasmic side of the cell membrane. by binding ligands they regulate the activity of a number of k(+) transporters and k(+) channels. to investigate the function of ktra from the bacterium vibrio alginolyticus (vaktra), the protein was overproduced in his-tagged form (his(10)-vaktra) and isolated by affinity chromatogr ...200717344221
the bidirectional polar and unidirectional lateral flagellar motors of vibrio alginolyticus are controlled by a single chey species.the bacterial flagellar motor is an elaborate molecular machine that converts ion-motive force into mechanical force (rotation). one of its remarkable features is its swift switching of the rotational direction or speed upon binding of the response regulator phospho-chey, which causes the changes in swimming that achieve chemotaxis. vibrio alginolyticus has dual flagellar systems: the na(+)-driven polar flagellum (pof) and the h(+)-driven lateral flagella (laf), which are used for swimming in li ...200717376072
growth response of vibrio cholerae and other vibrio spp. to cyanobacterial dissolved organic matter and temperature in brackish water.environmental control of growth and persistence of vibrios in aquatic environments is poorly understood even though members of the genus vibrio are globally important pathogens. to study how algal-derived organic matter and temperature influenced the abundance of different vibrio spp., baltic sea microcosms inoculated with vibrio cholerae, vibrio vulnificus, vibrio parahaemolyticus, vibrio alginolyticus and native bacterioplankton, were exposed to different temperatures (12-25 degrees c) and ame ...200717386033
characterization and resuscitation of viable but nonculturable vibrio alginolyticus vib283.the aim of this study was to investigate the viable but nonculturable (vbnc) state of the bacterium. vibrio alginolyticus vib283 was cultured in sterilized seawater microcosm at 4 degrees c. culturability of the cells in the microcosm was monitored by spread plate count (pc) on 2216e agar, pcs declined to undetectable levels (<0.1 cfu/ml) within 90 days. total cell counts remained constant throughout the period as determined by acridine orange direct count (aodc). the direct viable counts, on th ...200717492270
survival of vibrio alginolyticus in seawater and retention of virulence of its starved cells.the aim of this study was to evaluate the survival responses of four strains of vibrio alginolyticus in seawater under starvation conditions. we used microcosms containing sterilised seawater and incubated at ambient temperature (22-25 degrees c). v. alginolyticus maintained its culturability for at least nine months. long-term-starved cells showed an absence or a decrease in their enzymatic activities. resuscitation assays of viable but non-culturable (vbnc) cells were conducted and the recover ...200717524473
effect of the addition of four potential probiotic strains on the survival of pacific white shrimp (litopenaeus vannamei) following immersion challenge with vibrio parahaemolyticus.four bacterial strains isolated from the gastrointestinal tract of adult shrimp litopenaeus vannamei, vibrio alginolyticus utm 102, bacillus subtilis utm 126, roseobacter gallaeciensis slv03, and pseudomonas aestumarina slv22, were evaluated for potential use as probiotics for shrimp. in vitro studies demonstrated antagonism against the shrimp-pathogenic bacterium, vibrio parahaemolyticus ps-017. feeding shrimp with diets containing the potential probiotics showed the best feed conversion ratio ...200717544437
vibrios of the spotted rose snapper lutjanus guttatus steindachner, 1869 from northwestern characterize and identify vibrios present in wild and cultured juvenile snappers (lutjanus guttatus) in northwestern mexico.200717578416
[taxonomic composition of bacteria associated with cultivated mollusks crassostrea lugubris and perna viridis and with the water of the gulf of nha trang lagoon, vietnam].one hundred and four strains of heterotrophic bacteria have been isolated and characterized from two species of bivalve mollusks cultivated in the gulf of nha trang (vietnam) and from the water of a mariculture farm. the isolates have been identified on the basis of morphological, physiological, biochemical, and chemotaxonomic properties, as well as by the content of g+c bases in dna. in the microflora of mollusks, vibrio alginolyticus was predominant; the pathogenic species v. harveyi and v. sp ...200717583223
comparison of four antibiotics with indigenous marine bacillus spp. in controlling pathogenic bacteria from shrimp and artemia.use of antibiotics for the control of bacterial diseases in shrimp culture has caused several adverse impacts to the industry. this has resulted in the search for alternative environment friendly approaches to overcome bacterial infections. this study was conducted to investigate the use of beneficial bacteria as an alternative to antibiotics. ten pathogenic bacterial species isolated from shrimp, penaeus monodon, and artemia cysts were tested for susceptibility to indigenous marine bacillus sub ...200717584435
isolation, sequencing and characterization of cluster genes involved in the biosynthesis and utilization of the siderophore of marine fish pathogen vibrio fish pathogen vibrio alginolyticus mvp01, the isolated 11-gene cluster consisted of two divergently transcribed, fe(3+) and ferric uptake regulator (fur) regulated operons, pvsabcde and psua-pvuabcde, sharing high similarity with that related to siderophore biosynthesis and transportation locus in v. parahaemolyticus. siderophore biosynthesis or utilization was blocked when pvsa and pvsd of the pvsabcde operon or pvua, pvub and pvue of the psua-pvuabcde operon was single-gene in-frame mutated ...200717593352
molecular cloning and expression analysis of interferon-gamma inducible lysosomal thiol reductase (gilt)-like cdna from disk abalone (haliotis discus discus).interferon gamma (ifn-gamma) inducible lysosomal thiol reductase (gilt) has been described as a key enzyme in processing and presentation of major histocompatibility complex (mhc) class ii restricted antigen (ag) by catalyzing disulfide bond (s-s) reduction in mammals. abalone gilt-like (abgilt) full-length cdna was isolated from the normalized disk abalone cdna library. the 807-bp abgilt cdna consists of an open reading frame of 684-bp, encoding 228 amino acid residues. the predicted abgilt pro ...200717599346
rapid and specific identification of 5 human pathogenic vibrio species by multiplex polymerase chain reaction targeted to dnaj gene.a multiplex polymerase chain reaction (pcr) method, specifically designed for application in routine diagnostic laboratories, was developed for identifying 5 human pathogen vibrio species: vibrio cholerae, vibrio parahaemolyticus, vibrio vulnificus, vibrio mimicus, and vibrio alginolyticus. this assay directed toward the dnaj gene was tested on a total of 355 strains representing 13 vibrio species and 17 non-vibrio species. specific pcr fragments were produced in isolates belonging to the 5 targ ...200717614235
research and characterization of pathogenic vibrios from bathing water along the conero riviera (central italy).the occurrence and pathogenicity of vibrios in bathing water were investigated along the conero riviera (adriatic sea, central italy). vibrio spp. enumeration was performed on thiosulfate-citrate-bile-salts-sucrose-agar by the membrane filter method, and identification was done through a biochemical protocol. all isolates were tested for the presence of cytotoxicity, protease, lipase, elastase, gelatinase, urease, haemolytic activity, ctx, tdh and trh genes by conventional methods. in all, 200 v ...200717619048
cloning and expression of the gene encoding an extracellular alkaline serine protease from vibrio alginolyticus strain hy9901, the causative agent of vibriosis in lutjanus erythopterus (bloch).a 750-bp internal fragment of the alkaline serine protease gene (asp) from the vibrio alginolyticus strain hy9901 was amplified by polymerase chain reaction (pcr). the flanking sequences of the 5'- and 3'- ends of the asp gene were characterized by reverse and nested pcr. sequence analysis showed that the asp gene contained an 1893-bp orf encoding 630 amino acids. the deduced amino acid sequence of the asp (alkaline serine protease) precursor showed significant homology with several bacterial al ...200717640252
cloning and expression of gene encoding the thermostable direct hemolysin from vibrio alginolyticus strain hy9901, the causative agent of vibriosis of crimson snapper (lutjanus erythopterus).the main aims of this study were to clone and express complete open reading frame (orf) of thermostable direct haemolysin gene (tdh) from vibrio alginolyticus strain hy9901 in escherichia coli, and further evaluate the virulence of expressed tdh on mouse and crimson snapper.200717650188
[pathogenic vibrios in oysters (crassostrea rhizophorae) served at restaurants in rio de janeiro: a public health warning].forty oyster samples (crassostrea rhizophorae) served raw in 15 restaurants in the city of rio de janeiro were evaluated in order to investigate the presence of vibrio spp. the oyster samples were analyzed and subjected to enrichment in alkaline peptone water with the addition of 1 and 3% nacl and incubated at 37 degrees c for 24 hours. following this, the cultures were seeded onto thiosulfate citrate bile sucrose agar (tcbs) and the suspected colonies were subjected to biochemical characterizat ...200717653465
a secretory leukocyte proteinase inhibitor (slpi)-like protein from litopenaeus vannamei haemocytes.a partial clone coding for a two-wap domain protein was isolated from a litopenaeus vannamei haemocytes cdna library. the complete sequence was obtained by race, and the full-length cdna sequence is 0.8 kb long and encodes for a 116-amino acid protein. the domain composition is similar to the mammalian wfdc5 (wap four disulfide core) and secretory leukocyte proteinase inhibitor (slpi). modifications in expression were determined by real-time pcr, after injection of vibrio alginolyticus, suggesti ...200717664073
identification of a novel endochitinase from a marine bacterium vibrio proteolyticus strain no. 442.chitin binding proteins prepared from vibrio proteolyticus were purified and the n-terminal amino-acid sequence of a protein from a 110-kda band on sds-page was found to be 85-90% identical to the 22nd-41st residues of the n-termini of chitinase a precursor proteins from other vibrios. we cloned the corresponding gene, which encodes a putative protein of 850 amino acids containing a 26-residue signal sequence. the chitinase precursor from v. proteolyticus was 78-80% identical to those from vibri ...200717690022
characterization of the vibrio alginolyticusfur gene and localization of essential amino acid sites in fur by site-directed mutagenesis.the expression of iron-regulated genes in bacteria is typically controlled by the ferric uptake regulator (fur) protein, a global transcriptional repressor that regulates functions as diverse as iron acquisition, oxidative stress, virulence and acid tolerance. we have identified a fur homologue in vibrio alginolyticus and shown that it complements an escherichia coli fur mutant. reverse transcriptase pcr (rt-pcr) analysis proved that unlike many other fur homologues, v. alginolyticusfur is not u ...200717693709
immune stimulation and improved infection resistance in european sea bass (dicentrarchus labrax) fed mannan oligosaccharides.the objective of this study was to determine the effect of two levels of inclusion of mannan oligosaccharides derived from the outer cell wall of a select strain of saccharomyces cerevisiae (bio-mos, alltech inc, usa) on growth, feed utilization, immune status and disease resistance of european sea bass (dicentrarchus labrax). specimens of 35 g at initial density of 3 kg/m3 were fed during 67 days at 0 per thousand, 2 per thousand and 4 per thousand dietary mos level of inclusion in a commercial ...200717766145
molecular characterization of goose-type lysozyme homologue of large yellow croaker and its involvement in immune response induced by trivalent bacterial vaccine as an acute-phase protein.lysozyme acts as an innate immunity molecule against the invasion of bacterial pathogens. here, the cdna of a goose-type lysozyme (g-lysozyme) was cloned from large yellow croaker (pseudosciana crocea) by expressed sequence tags (est) and race-pcr techniques. the full-length cdna of large yellow croaker g-lysozyme (lycgl) is 716 nucleotides (nt) encoding a protein of 193 amino acids (aa), with a theoretical molecular weight of 21.3kda. the deduced lycgl possessed the typical structural features ...200717850883
a novel multiplex pcr for the identification of vibrio parahaemolyticus, vibrio cholerae and vibrio establish a simple multiplex polymerase chain reaction (pcr) that will identify vibrio parahaemolyticus, vibrio cholerae and vibrio vulnificus.200717897378
expression and immunogenicity analysis of two iron-regulated outer membrane proteins of vibrio parahaemolyticus.genes of two iron-regulated outer membrane proteins of vibrio parahaemolyticus zj2003, a pathogenic strain isolated from large yellow croaker (pseudosciaena crocea), psua and pvua, were cloned and expressed as n-terminal his(6)-tagged proteins in escherichia coli bl(21)(de(3)). the recombinant fusion proteins were purified with nickel chelate affinity chromatography. to analyze the immunogenicity of the proteins, groups of large yellow croaker were immunized with the purified recombinant psua, p ...200717928925
luxo controls extracellular protease, haemolytic activities and siderophore production in fish pathogen vibrio characterize the luxo gene in fish pathogen vibrio alginolyticus mvp01 and investigate its roles in regulation of extracellular products (ecp) and siderophore production.200717953563
development of a sybr green i real-time pcr for quantitative detection of vibrio alginolyticus in seawater and seafood.vibrio alginolyticus is an economically important micro-organism. the main aim of the present study was to develop a real-time polymerase chain reaction (pcr) assay for rapid, sensitive and effective quantification of v. alginolyticus in seawater and seafood.200717953599
oil-utilizing bacteria associated with fish from the arabian gulf.the objectives were to count and identify the oil-utilizing bacteria associated with fish, and to study their hydrocarbon-degradation potential.200717953689
[characteristics of adhesion of epiphytic bacteria on leaves of the seagrass zostera marina and on abiotic surfaces].a comparative study of the adhesion of epiphytic bacteria and marine free-living, saprophytic, and pathogenic bacteria on seagrass leaves and abiotic surfaces was performed to prove the occurrence of true epiphytes of zostera marina and to elucidate the bacterium-plant symbiotrophic relationships. it was shown that in the course of adhesion to the seagrass leaves of two taxonomically different bacteria, cytophaga sp. kmm 3552 and pseudoalteromonas citrea kmm 461, isolated from the seagrass surfa ...200717974207
dietary administration of sodium alginate and kappa-carrageenan enhances the innate immune response of brown-marbled grouper epinephelus fuscoguttatus and its resistance against vibrio alginolyticus.brown-marbled grouper epinephelus fuscoguttatus which had been fed diets containing sodium alginate and kappa (kappa)-carrageenan at 5, 10 and 20gkg(-1), respectively after 0, 2, 4, 6, 8 and 14 weeks were examined for survival, growth, innate cellular and humoral responses as compared to the fish that fed control non-supplemented diet. survival was 100% for the fish that fed all diets after 14 weeks and no significant difference in growth was observed among seven diets. the fish that fed a diet ...200817981341
effect of saponin immersion on enhancement of the immune response of white shrimp litopenaeus vannamei and its resistance against vibrio alginolyticus.the haemocyte count, phenoloxidase (po) activity, specific alpha(2)-macroglobulin (alpha2-m) activity, respiratory burst, superoxide dismutase (sod) activity, glutathione peroxidase (gpx) activity, phagocytic activity, and clearance efficiency against vibrio alginolyticus were examined when the white shrimp litopenaeus vannamei (10.42+/-2.0g) were immersed in seawater (34 per thousand) containing different concentrations of saponin (0, 0.5, 1 and 2mgl(-1)) for 24, 48 and 72h. hyaline cells (hc), ...200818032066
genes encoding the vibrio harveyi haemolysin (vhh)/thermolabile haemolysin (tlh) are widespread in vibrios.v. harveyi vhh haemolysin, which shows high homology to the tlh haemolysin (the identities of their deduced amino acid sequences are up to 85.6%), is a putative virulence factor to marine cultured fish. a vhh probe, which is specific to v. harveyi vhha haemolysin gene, was used to screen ecor i digests of total dna from 57 vibrio strains, including 26 vibrio type strains, 20 v. harveyi isolates and 11 v. parahaemolyticus isolates. as a result, 1 strong hybridisation band was detected in 13 type ...200718062266
molecular cloning of leucocyte cell-derived chemotaxin-2 gene in croceine croaker (pseudosciaena crocea).leucocyte cell-derived chemotaxin-2 (lect2) was originally demonstrated to have a chemotactic activity against human neutrophils in vitro. current evidence suggests that lect2 may be a multifunctional protein involved in cell growth, differentiation and autoimmune. a full-length cdna clone of the lect2 gene, 595bp in size, was isolated from the fish croceine croaker (pseudosciaena crocea). it's 3'-utr was much shorter (112nts) than that of trout lect2 gene (210nts). its deduced amino acid sequen ...200818155922
complement-mediated killing of vibrio species by the humoral fluids of amphioxus branchiostoma belcheri: implications for a dual role of o-antigens in the resistance to bactericidal activity.the functional properties of complement in invertebrate deuterostomes are rather ill-defined. here we showed that the humoral fluids from amphioxus branchiostoma belcheri were capable of causing lysis of some vibrio species including vibrio alginolyticus hw284, vibrio parahaemolyticus hw458 and vibrio harvey sf-1, the first such data in the invertebrate deuterostomes. the fluid bacteriolytic activity was abolished by pre-incubation with heat-inactivated rabbit anti-human c3 serum, heating at 45 ...200818178103
torque-speed relationships of na+-driven chimeric flagellar motors in escherichia coli.the bacterial flagellar motor is a rotary motor in the cell envelope of bacteria that couples ion flow across the cytoplasmic membrane to torque generation by independent stators anchored to the cell wall. the recent observation of stepwise rotation of a na(+)-driven chimeric motor in escherichia coli promises to reveal the mechanism of the motor in unprecedented detail. we measured torque-speed relationships of this chimeric motor using back focal plane interferometry of polystyrene beads attac ...200818207160
vibrios in association with sedimentary crustaceans in three beaches of the northern adriatic sea (italy).in the marine environment, vibrios adhere to a number of substrates including chitin-rich organisms such as crustaceans. their wide diffusion in coastal waters and pathogenic potential require knowledge of the lifestyle and environmental reservoirs of these bacteria. to test the presence of culturable vibrios in coastal areas and their association with benthic crustaceans, vibrios were isolated from water, sediments and crustaceans (copepods and anphipods) at three stations placed in front of he ...200818243247
regulation of vibrio alginolyticus virulence by the luxs quorum-sensing system.quorum sensing (qs) is a bacterial intercommunication system that controls the expression of multiple genes in response to population density. the luxs qs system regulates the expression of several virulence factors in a wide variety of pathogenic bacteria. luxs has been characterized to be responsible for producing a type of autoinducer, ai-2, which stimulates the expression of the luciferase operon in vibrio harveyi. vibrio alginolyticus is established as an opportunistic pathogen of several m ...200818261029
the na+-translocating nadh:quinone oxidoreductase (na+-nqr) from vibrio cholerae enhances insertion of fes in overproduced nqrf subunit.the na+-translocating nadh:quinone oxidoreductase (na+-nqr) from vibrio cholerae is a membrane-bound, respiratory na+ pump. its nqrf subunit contains one fad and a [2fe-2s] cluster and catalyzes the initial oxidation of nadh. a soluble variant of nqrf lacking its hydrophobic, n-terminal helix (nqrf') was produced in v. cholerae wild type and nqr deletion strain. under identical conditions of growth and induction, the yield of nqrf' increased by 30% in the presence of the na+-nqr. fad-containing ...200818289689
environmental vibrio spp., isolated in mozambique, contain a polymorphic group of integrative conjugative elements and class 1 integrons.circulation of mobile genetic elements linked to drug resistance spread was studied in vibrio strains isolated from surface urban water (river and sea) and shellfish samples in 2002-2003 in maputo, mozambique. class 1 integrons and integrating conjugative elements (ice) were investigated by pcr and mating experiments in strains of major health interest: 10 vibrio cholerae, six vibrio parahaemolyticus, two vibrio alginolyticus and one vibrio fluvialis. resistance to at least two antibiotics (pred ...200818318712
roles of charged residues in the c-terminal region of poma, a stator component of the na+-driven flagellar motor.bacterial flagellar motors use specific ion gradients to drive their rotation. it has been suggested that the electrostatic interactions between charged residues of the stator and rotor proteins are important for rotation in escherichia coli. mutational studies have indicated that the na(+)-driven motor of vibrio alginolyticus may incorporate interactions similar to those of the e. coli motor, but the other electrostatic interactions between the rotor and stator proteins may occur in the na(+)-d ...200818326582
influence of beta-glucans on the immune responses of carpet shell clam (ruditapes decussatus) and mediterranean mussel (mytilus galloprovincialis).the effects of beta-glucans on several immune functions of carpet shell clam (ruditapes decussatus) and mediterranean mussel (mytilus galloprovincialis) hemocytes were determined. nitric oxide (no) production increased significantly in beta-glucan treated mussels and clams. in mussels, beta-glucans increased by themselves the release of free oxygen radicals and also were able to enhance the phorbol 12-myristate 13-acetate (pma) mediated effect on this hemocyte activity. however, high doses of be ...200818329901
a novel c-type lectin from abalone, haliotis discus discus, agglutinates vibrio alginolyticus.owing to its specific binding to carbohydrates, lectins play important roles in pathogen recognition and clearance in invertebrate animals. in this study, a novel c-type lectin (designated clhd) gene was isolated from abalone, haliotis discus discus, cdna library. the complete cdna sequence of the clhd gene is 508 base pairs in length, and encodes 151 amino acids. clhd shares a highly conserved carbohydrate recognition domain with c-type lectins from mollusk and fish. the mrna expressions of clh ...200818372041
[cutaneous ulceration after an octopus bite: infection due to vibrio alginolyticus, an emerging pathogen].vibrio alginolyticus is an anaerobic gram-negative bacillus found in normal marine flora and is considered as non pathogenic for humans. infections due to vibrio alginolyticus are seldom reported and can be seen after exposure to seawater or to marine animals.200818374857
involvement of luxs in the regulation of motility and flagella biogenesis in vibrio alginolyticus.the fish pathogen vibrio alginolyticus contains two unique flagellar systems. the luxs quorum sensing system is reported to regulate the expression of virulence factors in a wide variety of pathogenic bacteria. our previous work demonstrated that inactive luxs led to decreased virulence in v. alginolyticus. in this study, luxs-dependent regulation of motility and flagella biogenesis, the potential virulence factors in v. alginolyticus, were further investigated. a luxs-deleted mutant showed defi ...200818391446
collaboration of flhf and flhg to regulate polar-flagella number and localization in vibrio alginolyticus.precise regulation of the number and placement of flagella is critical for the mono-polar-flagellated bacterium vibrio alginolyticus to swim efficiently. we have shown previously that the number of polar flagella is positively regulated by flhf and negatively regulated by flhg. we now show that deltaflhf cells are non-flagellated as are most deltaflhfg cells; however, some of the deltaflhfg cells have several flagella at lateral positions. we found that flhf-gfp was localized at the flagellated ...200818451048
septic shock due to vibrio alginolyticus in a cirrhotic patient: the first case in korea.we describe a case of septic shock due to vibrio alginolyticus presenting with fever and bilateral leg pain. despite intensive management with antibiotics and inotropic agents, the patient died from septic shock 1 day after hospitalization. v. alginolyticus was isolated from both leg wounds and a blood culture. to the best of our knowledge, this is the first reported case of v. alginolyticus bacteremia in korea.200818452273
insights into the stator assembly of the vibrio flagellar motor from the crystal structure of moty.rotation of the sodium-driven polar flagellum of vibrio alginolyticus requires four motor proteins: poma, pomb, motx, and moty. poma and pomb form a sodium-ion channel in the cytoplasmic membrane that functions as a stator complex to couple sodium-ion flux with torque generation. motx and moty are components of the t-ring, which is located beneath the p-ring of the polar flagellar basal body and is involved in incorporation of the poma/pomb complex into the motor. here, we describe the determina ...200818505842
chemical investigation of finlaysonia obovata: part i--a rare triterpene acid showing antibacterial activity against fish pathogens.the antibacterial screening of extracts of the leaves of finlaysonia obovata with hexane, chloroform and alcohol was carried out against fresh water fish pathogenic bacteria viz., micrococcus sp. (multidrug resistant strain), aeromonas hydrophila, pseudomonas aeruginosa, vibrio alginolyticus, staphylococcus aureus, escherichia coli, edwardsiella tarda by disc-assay method. the hexane and chloroform extracts were found active against four and five pathogens, respectively. the highly active chloro ...200818569719
roles of luxr in regulating extracellular alkaline serine protease a, extracellular polysaccharide and mobility of vibrio marine vibrio species, the vibrio harveyi-type luxr protein, a key player in a quorum-sensing system, controls the expression of various genes. in this study, the luxr homologue in vibrio alginolyticus was identified and named luxr(val), whose expression was greatly induced by the increase of cell number. the luxr(val) in-frame deletion mutant showed a significant downregulation of total extracellular protease activity, and especially caused a 70% decrease in the transcript levels of extracel ...200818573155
genotyping of vibrio alginolyticus isolates from daya bay by infrequent-restriction-site pcr and pulsed-field gel electrophoresis.vibrio alginolyticus is a serious bacterial pathogen that hampered the whole industry of fish farming in guangdong, china. in order to facilitate epidemiologic studies and improve the control of disease, we developed a highly efficient method of genotyping for v. alginolyticus using infrequent-restriction-site pcr (irs-pcr) technology. with the enzyme combination of noti-hhai, optimized, unique, and easy-to-interpret patterns were generated. forty-five v. alginolyticus isolates from aquatic anim ...200818586455
characterization of two tonb systems in marine fish pathogen vibrio alginolyticus: their roles in iron utilization and gram-negative bacteria, the tonb/exbb/exbd complex is required to energize the specific high-affinity receptors mediating iron uptake processes. in fish pathogen vibrio alginolyticus mvp01, which exhibited capacities to scavenge iron sources with various specific iron uptake systems, we identified and characterized two sets of tonb systems. in v. alginolyticus, tonb1 and tonb2 systems were arrayed as tonb1-exbb1-exbd1 and exbb2-exbd2-tonb2, respectively. the transcription of the tonb systems ...200818629473
role of rpos in stress survival, synthesis of extracellular autoinducer 2, and virulence in vibrio alginolyticus.vibrio alginolyticus, a marine bacterium, is an opportunistic pathogen capable of causing vibriosis with high mortality to fishes in the south china sea. stress resistance is very important for its survival in the natural environment and upon infection of the host. rpos, an alternative sigma factor, is considered as an important regulator involved in stress response and virulence in many pathogens. in this study, the rpos gene was cloned and characterized to evaluate the role of rpos in v. algin ...200818641971
vibrios dominate as culturable nitrogen-fixing bacteria of the brazilian coral mussismilia hispida.taxonomic characterization was performed on the putative n(2)-fixing microbiota associated with the coral species mussismilia hispida, and with its sympatric species palythoa caribaeorum, p. variabilis, and zoanthus solanderi, off the coast of são sebastião (são paulo state, brazil). the 95 isolates belonged to the gammaproteobacteria according to the 16s rdna gene sequences. in order to identify the isolates unambiguously, pyrh gene sequencing was carried out. the majority of the isolates (n = ...200818678453
effects of rutin from toona sinensis on the immune and physiological responses of white shrimp (litopenaeus vannamei) under vibrio alginolyticus challenge.rutin is a bioflavonoid with strong antioxidant activity. to investigate the regulatory roles of rutin in various functions in crustaceans, we examined physiological (haemolymph glucose, lactate, and lipid) and innate non-specific immune responses (total haemocyte count (thc), phenoloxidase activity (po), respiratory bursts (release of superoxide anion, o(2)(-)) and superoxide dismutase (sod) activity) to the pathogen vibrio alginolyticus in white shrimp (litopenaeus vannamei) that were individu ...200818718869
[isolation and physiological characteristics of lytic bacteriophages of vibrio].vibrio is a widely distributed pathogen in aquatic environment. our study aimed at searching for possible biological control of pathogenic vibrio.200818720843
transcriptional up-regulation of disk abalone selenium dependent glutathione peroxidase by h(2)o(2) oxidative stress and vibrio alginolyticus bacterial infection.selenium dependent glutathione peroxidase (se-gpx) belongs to the family of selenoprotein, which acts mainly as an antioxidant in the cellular defence system. we have identified se-gpx full length cdna from disk abalone (haliotis discus discus) designated as abse-gpx. it has a characteristic codon at (223)tga(225) that corresponds to selenocysteine (sec) amino acid as u(75). the full length cdna consists of 675 bp, an open reading frame encoding 225 amino acids. sequence characterization reveale ...200818722791
enzymatic assay of marine bacterial phosphatases by capillary electrophoresis with laser-induced fluorescence detection.microbial ectoenzyme activities in aquatic environments are important determinants of polymer hydrolysis and indicators of the state of microbial carbon, nitrogen, and phosphorus nutrition. marine ectoenzymes are found on the cell surface or in the periplasmic space of gram-negative heterotrophic bacteria. phosphatases, which remove phosphate groups from substrates, are one example of an ectoenzyme. enzyme assays based on-capillary electrophoresis (ce) take advantage of ce's high-efficiency sepa ...200818726971
cell-free synthesis of the torque-generating membrane proteins, poma and pomb, of the na+-driven flagellar motor in vibrio alginolyticus.flagellar motor proteins, poma and pomb, are essential for converting the sodium motive force into rotational energy in the na(+)-driven flagella motor of vibrio alginolyticus. poma and pomb, which are cytoplasmic membrane proteins, together comprise the stator complex of the motor and form a na(+) channel. we tried to synthesize poma and pomb by using the cell-free protein synthesis system, puresystem. we succeeded in doing so in the presence of liposomes, and showed an interaction between them ...200818776205
molecular characterization and gene expression analysis of a pattern recognition protein from disk abalone, haliotis discus discus.pattern recognition molecules play an important role in innate immunity by recognizing common epitopes on the surface of invading microorganism. a pattern recognition protein (prp) was isolated from a disk abalone, haliotis discus discus, normalized cdna library. it encodes 420 amino acids (aa) including a 20 aa signal peptide sequence. the mature protein has an estimated molecular mass of 45kda and predicted pi of 5.0. the deduced aa sequence showed greatest homology (50%) with a beta-glucan re ...200818782621
expression and characterization of the biofilm-related and carnosine-hydrolyzing aminoacylhistidine dipeptidase from vibrio alginolyticus.the biofilm-related and carnosine-hydrolyzing aminoacylhistidine dipeptidase (pepd) gene from vibrio alginolyticus was cloned and sequenced. the recombinant pepd protein was produced and biochemically characterized and the putative active-site residues responsible for metal binding and catalysis were identified. the recombinant enzyme, which was identified as a homodimeric dipeptidase in solution, exhibited broad substrate specificity for xaa-his and his-xaa dipeptides, with the highest activity ...200818783432
molecular cloning, characterization, expression and antibacterial analysis of a lysozyme homologue from fenneropenaeus merguiensis.the gene coding for lysozyme in banana prawn (fenneropenaeus merguiensis) was cloned, sequenced and expressed in pet-32a vector. the deduced amino acid sequence of f. merguiensis lysozyme showed 37-93% similarity with the mouse, human, chicken, and tiger prawn counterparts. the lysozyme was purified to homogeneity and observed as a band of approximately 15 kda in 12% sds-page. semiquantitative rt-pcr analysis demonstrated that mrna transcripts of lysozyme could be mainly detected in the tissues ...200918798009
the vibrio core group induces yellow band disease in caribbean and indo-pacific reef-building determine the relationship between yellow band disease (ybd)-associated pathogenic bacteria found in both caribbean and indo-pacific reefs, and the virulence of these pathogens. ybd is one of the most significant coral diseases of the tropics.200818798767
enzymatic characterization of vibrio alginolyticus strains isolated from bivalves harvested at venice lagoon (italy) and guanabara bay (brazil).the aquatic ecosystem is the natural habitat of microorganisms including vibrio and aeromonas genus which are pathogenic to human and animals. in the present investigation the frequency of these bacteria and the enzymatic characteristics of 34 vibrio alginolyticus strains isolated from bivalves harvested in venice lagoon (italy) and guanabara bay (brazil) were carried out from november 2003 to february 2004. the mussels' samples were submitted to enrichment in alkaline peptone water (apw) added ...200818813756
portal of entry for pathogenic vibrio alginolyticus into large yellow croaker pseudosciaena crocea, and characteristics of bacterial adhesion to mucus.the portal of entry for pathogenic vibrio alginolyticus into large yellow croaker pseudosciaena crocea is via the intestinal tract rather than gill or skin according to the kinetics of the bacterial adhesion to different mucus. the different effects on adhesion caused by proteolytic enzymes and heat treatment might be due to the different chemical compositions of mucus. adhesion of v. alginolyticus to mucus depends on concerted action of bacterial surface structures such as cell-surface proteins ...200818814543
molecular and functional characterization of a novel cc chemokine in large yellow croaker (pseudosciaena crocea).a full-length cc chemokine cdna was isolated from large yellow croaker (pseudosciaena crocea) by expressed sequence tag (est) analysis (lyccc2). its open reading frame (orf) is 282 nucleotides (nt), encoding a polypeptide of 93 amino acids (aa). the deduced lyccc2 contains a 20-aa signal peptide and a 73-aa mature polypeptide, which possesses the typical arrangement of four cysteines as found in other known cc chemokines (c(34), c(35), c(61) and c(75)). it shares 17.4-40.4% and 10.0-24.5% aa seq ...200818835451
the effect of vibrio alginolyticus infection on caspase-3 expression and activity in white shrimp litopenaeus vannamei.complementary (c)dna encoding cysteine aspartate protease-3 (caspase-3) messenger (m)rna of the white shrimp litopenaeus vannamei was obtained from haemocytes by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race) using oligonucleotide primers based on the caspase sequences of penaeus merguiensis, spodoptera frugiperda, spodoptera littoralis, and penaeus monodon. the 1212-bp cdna contained an open reading frame (orf) of 951bp, a 56-bp 5'-untrans ...200818840531
the immune response of white shrimp litopenaeus vannamei following vibrio alginolyticus injection.white shrimp litopenaeus vannamei injected with saline, and injected with tryptic soy broth (tsb)-grown vibrio alginolyticus at 1.0 x 10(5) and 1.8 x 10(5) colony-forming units (cfu) shrimp(-1) were examined for hyaline cell (hc) counts, granular cell (gc) counts, total haemocyte counts (thcs), phenoloxidase (po) activity, respiratory burst (rb) and superoxide dismutase (sod) activity after 1-168 h. shrimp that received no injection served as the control. the shrimps which received v. alginolyti ...200818951981
characterization of the bpi-like gene from a subtracted cdna library of large yellow croaker (pseudosciaena crocea) and induced expression by formalin-inactivated vibrio alginolyticus and nocardia seriolae vaccine expressed sequence tag (est 64lf004 clone), which is from the subtracted cdna library of the head kidney of large yellow croaker (pseudosciaena crocea) stimulated with peptidoglycan (pg) by suppression subtractive hybridization (ssh) method, was cloned using race-pcr. the full length cdna, which possesses typical structural features of a signal peptide, a conserved lps binding domain and two bactericidal permeability-increasing (bpi) motifs as in higher vertebrates, was identified as a novel ...200818952461
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