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the immune response of white shrimp litopenaeus vannamei and its susceptibility to vibrio alginolyticus at different salinity levels.white shrimp litopenaeus vannamei held in 25 per thousand seawater were injected with tsb-grown vibrio alginolyticus (1 x 10(4) cfu shrimp(-1)), and then transferred to 5, 15, 25 (control) and 35 per thousand. over 24-96 h, the mortality of v. alginolyticus-injected shrimp held in 5 per thousand and 15 per thousand was significantly higher than that of shrimp held in 25 per thousand and 35 per thousand, and the mortality of v. alginolyticus-injected shrimp held in 5 per thousand was the highest. ...200515561558
molecular cloning and characterisation of a pattern recognition molecule, lipopolysaccharide- and beta-1,3-glucan binding protein (lgbp) from the white shrimp litopenaeus vannamei.a lipopolysaccharide- and beta-1,3-glucan binding protein (lgbp) cdna was cloned from the haemocyte and hepatopancreas of white shrimp litopenaeus vannamei using oligonucleotide primers and rt-pcr. both 3'- and 5'-regions were isolated by rapid amplification of cdna end race method. analysis of nucleotide sequence revealed that the cdna clone has an open reading frame of 1101 bp encoding a protein of 367 amino acids including a 17 amino acid signal peptide. the calculated molecular mass of the m ...200515561560
equilibrium unfolding of an oligomeric protein involves formation of a multimeric intermediate state(s).superoxide dismutases (sods) are important metalloenzymes which protect cells against oxidative stress by scavenging reactive superoxides. missense mutations in sods are known to lead to some familial cases of amyotrophic lateral sclerosis and several forms of cancers. in the present study, we investigate the guanidinium hydrochloride (gdnhcl)-induced equilibrium unfolding of apo-manganese superoxide dismutase (apo-mnsod) isolated from vibrio alginolyticus using a variety of biophysical techniqu ...200515567159
dietary administration of sodium alginate enhances the immune ability of white shrimp litopenaeus vannamei and its resistance against vibrio alginolyticus.haemocyte count, phenoloxidase activity, respiratory burst (release of superoxide anion), superoxide dismutase (sod) activity, glutathione peroxidase (gpx) activity, phagocytic activity and clearance efficiency to the pathogen vibrio alginolyticus were measured in white shrimp litopenaeus vannamei juveniles (12.3 +/- 1.2 g) which had been fed diets containing sodium alginate at 0.5, 1.0, 2.0 g kg(-1) after five months. l. vannamei fed a diet containing 2.0 g kg(-1) sodium alginate had increased ...200515450964
clearance rates of sabella spallanzanii and branchiomma luctuosum (annelida: polychaeta) on a pure culture of vibrio alginolyticus.the influence exerted by filter-feeding activity on bacterial density by two sabellid species from the mediterranean sea (ionian sea, italy), branchiomma luctuosum grube and sabella spallanzanii gmelin (annelida: polychaeta) was investigated. clearance rates and retention efficiencies were estimated utilizing the species vibrio alginolyticus selected on account of previous field studies and its importance in fish culture pathogenicity. the cmax was 43.2+/-2.63 l h(-1) g(-1) dw for b. luctuosum a ...200516242172
[studies on extracellular virulent factors produced by potential pathogenic bacteria isolated from abalone postlarvae and biofilms].to find out the potential pathogen(s) that caused massive death of abalone postlarvae( haliotis diversicolor supertesta ) in southern china, 105 bacterial strains were isolated from the water, whitened postlarvae and their biofilms of an abalone farm in guangdong province. extra-cellular protease, gelatinase, lipase as well as haemolysis tests were performed on them. lysophospholipase (tlh)-targeted pcr was also carried out in order to reveal if the haemolysis caused by bacterial strains were re ...200516245888
biological deinking of inkjet-printed paper using vibrio alginolyticus and its enzymes.recycling of office waste paper (photocopy, inkjet, and laser prints) is a major problem due to difficulty in removal of nonimpact ink. biological deinking of office waste paper is reported using several microorganisms and their enzymes. we report here deinking and decolorization of the dislodged ink particles from inkjet printed paper pulp by a marine bacterium, vibrio alginolyticus isolate no. nio/di/32, obtained from marine sediments. decolorization of this pulp was achieved within 72 h by gr ...200516133100
a fluid-dynamic interpretation of the asymmetric motion of singly flagellated bacteria swimming close to a boundary.the singly flagellated bacterium, vibrio alginolyticus, moves forward and backward by alternating the rotational direction of its flagellum. the bacterium has been observed retracing a previous path almost exactly and swimming in a zigzag pattern. in the presence of a boundary, however, the motion changes significantly, to something closer to a circular trajectory. additionally, when the cell swims close to a wall, the forward and backward speeds differ noticeably. this study details a boundary ...200516150964
direct observation of steps in rotation of the bacterial flagellar motor.the bacterial flagellar motor is a rotary molecular machine that rotates the helical filaments that propel many species of swimming bacteria. the rotor is a set of rings up to 45 nm in diameter in the cytoplasmic membrane; the stator contains about ten torque-generating units anchored to the cell wall at the perimeter of the rotor. the free-energy source for the motor is an inward-directed electrochemical gradient of ions across the cytoplasmic membrane, the protonmotive force or sodium-motive f ...200516208378
all four putative selectivity filter glycine residues in ktrb are essential for high affinity and selective k+ uptake by the ktrab system from vibrio alginolyticus.the subunit ktrb of bacterial na+-dependent k+-translocating ktrab systems belongs to a superfamily of k+ transporters. these proteins contain four repeated domains, each composed of two transmembrane helices connected by a putative pore loop (p-loop). the four p-loops harbor a conserved glycine residue at a position equivalent to a glycine selectivity filter residue in k+ channels. we investigated whether these glycines also form a selectivity filter in ktrb. the single residues gly70, gly185, ...200516210320
a marine bacterium, micrococcus mccb 104, antagonistic to vibrios in prawn larval rearing systems.a marine bacterium, micrococcus mccb 104, isolated from hatchery water, demonstrated extracellular antagonistic properties against vibrio alginolyticus, v. parahaemolyticus, v. vulnificus, v. fluviallis, v. nereis, v. proteolyticus, v. mediterranei, v cholerae and aeromonas sp., bacteria associated with macrobrachium rosenbergii larval rearing systems. the isolate inhibited the growth of v. alginolyticus during co-culture. the antagonistic component of the extracellular product was heat-stable a ...200516465832
cloning and expression analysis of interferon-gamma-inducible-lysosomal thiol reductase gene in large yellow croaker (pseudosciaena crocea).in mammals, interferon-gamma-inducible-lysosomal thiol reductase (gilt) has been demonstrated to play a key role in the processing and presentation of mhc class ii-restricted antigen (ag) by catalyzing disulfide bond reduction, thus unfolding native protein ag and facilitating subsequent cleavage by proteases. here, we reported the cloning of a gilt gene homologue from the spleen of large yellow croaker, a marine fish (lycgilt). the full-length cdna of lycgilt gene is 1033 nucleotides (nt) encod ...200616478632
crystallization of the nadh-oxidizing domain of the na+-translocating nadh:ubiquinone oxidoreductase from vibrio cholerae.the na+-translocating nadh:quinone oxidoreductase (na+-nqr) from pathogenic and marine bacteria is a respiratory complex that couples the exergonic oxidation of nadh by quinone to the transport of na+ across the membrane. the nqrf subunit oxidizes nadh and transfers the electrons to other redox cofactors in the enzyme. the fad-containing domain of nqrf has been expressed, purified and crystallized. the purified nqrf fad domain exhibited high rates of nadh oxidation and contained stoichiometric a ...200616511277
molecular cloning of growth hormone from silver sea bream: effects of abiotic and biotic stress on transcriptional and translational expression.the pituitary growth hormone (gh) gene of silver sea bream (sparus sarba) was cloned and characterized and found to be 615 base pairs encoding a protein of 204 amino acids. using a bacterial expression system, recombinant protein was prepared and rabbit polyclonal antibody was raised. transcript and protein amounts of gh were measured in fish that were adapted to a range of salinities, acclimated to different temperatures, or undergoing a natural time course of vibrio alginolyticus infection. is ...200616513086
use of multiparameter analysis for vibrio alginolyticus viable but nonculturable state determination.vibrio alginolyticus is known to enter into a viable but nonculturable (vbnc) state in response to environmental conditions unfavorable to the growth. cells in vbnc condition pose a public health threat because they are potentially pathogenic.200616528721
isolation of a pathogenic strain of vibrio alginolyticus from necrotic larvae of macrobrachium rosenbergii (de man). 200616533305
affinity proteomic approach for identification of an iga-like protein in litopenaeus vannamei and study on its agglutination characterization.an unknown protein reacted with anti-human iga, namely, iga-like protein, has been reported in shrimp, but information regarding its identification is not available. in the present study, an affinity proteomic strategy was applied to identify the iga-like protein of shrimp litopenaeus vannamei. the protein of 75 kda was isolated and confirmed by affinity chromatography and western blotting with goat anti-human iga, respectively, and then identified as hemocyanin, a member of igsf, by mass spectr ...200616602688
soft-agar-coated filter method for early detection of viable and thermostable direct hemolysin (tdh)- or tdh-related hemolysin-producing vibrio parahaemolyticus in seafood.a novel method for detecting viable and thermostable direct hemolysin (tdh)-producing or tdh-related hemolysin (trh)-producing vibrio parahaemolyticus in seafood was developed. the method involved (i) enrichment culture, selective for viable, motile cells penetrating a soft-agar-coated filter paper, and (ii) a multiplex pcr assay targeting both the tdh gene (tdh) and trh gene (trh) following dnase pretreatment on the test culture to eradicate any incidental dnas that might have been released fro ...200616820446
low flagellar motor torque and high swimming efficiency of caulobacter crescentus swarmer cells.we determined the torque of the flagellar motor of caulobacter crescentus for different motor rotation rates by measuring the rotation rate and swimming speed of the cell body and found it to be remarkably different from that of other bacteria, such as escherichia coli and vibrio alginolyticus. the average stall torque of the caulobacter flagellar motor was approximately 350 pn nm, much smaller than the values of the other bacteria measured. furthermore, the torque of the motor remained constant ...200616844761
virulence and enterobacterial repetitive intergenic consensus pcr of vibrio alginolyticus strains isolated from tunisian cultured gilthead sea bream and sea bass outbreaks.vibrio alginolyticus was isolated from the internal organs of diseased gilthead sea bream (sparus aurata) and sea bass (dicentrarchus labrax) cultured in two fish farms located on the tunisian mediterranean coast, from 2003 to 2005. after phenotypic characterisation, a selection of 34 isolates from gilthead sea bream and sea bass were molecularly typed by repetitive intergenic consensus pcr (eric-pcr) showing a high polymorphism among the isolated strains (19 genotypes). most of the isolates wer ...200616870360
salmonella spp., vibrio spp., clostridium perfringens, and plesiomonas shigelloides in marine and freshwater invertebrates from coastal california ecosystems.the coastal ecosystems of california are highly utilized by humans and animals, but the ecology of fecal bacteria at the land-sea interface is not well understood. this study evaluated the distribution of potentially pathogenic bacteria in invertebrates from linked marine, estuarine, and freshwater ecosystems in central california. a variety of filter-feeding clams, mussels, worms, and crab tissues were selectively cultured for salmonella spp., campylobacter spp., escherichia coli-o157, clostrid ...200616897302
development of monoclonal antibodies for simple identification of vibrio alginolyticus.the present study was aimed to produce monoclonal antibodies (mabs) for simple and specific identification of vibrio alginolyticus infection in shrimp.200616965376
the vibrio motor proteins, motx and moty, are associated with the basal body of na-driven flagella and required for stator formation.the four motor proteins poma, pomb, motx and moty, which are believed to be stator proteins, are essential for motility by the na(+)-driven flagella of vibrio alginolyticus. when we purified the flagellar basal bodies, motx and moty were detected in the basal body, which is the supramolecular complex comprised of the rotor and the bushing, but poma and pomb were not. by antibody labelling, motx and moty were detected around the lp ring. these results indicate that motx and moty associate with th ...200617038120
characterization of a vibrio alginolyticus strain, isolated from alaskan oysters, carrying a hemolysin gene similar to the thermostable direct hemolysin-related hemolysin gene (trh) of vibrio parahaemolyticus.a vibrio strain isolated from alaskan oysters and classified by its biochemical characteristics as vibrio alginolyticus possessed a thermostable direct hemolysin-related hemolysin (trh) gene previously reported only in vibrio parahaemolyticus. this trh-like gene was cloned and sequenced and was 98% identical to the trh2 gene of v. parahaemolyticus. this gene seems to be functional since it was transcriptionally active in early-stationary-phase growing cells. to our knowledge, this is the first r ...200617056701
aerobic microorganisms associated with free-ranging bottlenose dolphins in coastal gulf of mexico and atlantic ocean waters.our abilities to assess health risks to free-ranging dolphin populations, to treat live-stranded or captive dolphins, and to evaluate the risks of disease transmission between humans and dolphins have suffered from a lack of basic information on microorganisms associated with normal, presumably healthy free-ranging individuals. in order to provide these data, we sampled free-ranging bottlenose dolphins (tursiops truncatus) off florida, texas, and north carolina during 1990-2002. blowhole and ana ...200617092884
humoral immune responses of amphioxus branchiostoma belcheri to challenge with escherichia coli.humoral parameters of amphioxus branchiostoma belcheri, including lysozyme, antimicrobial activity, microbial agglutinin and haemagglutinins were measured before and after challenge with escherichia coli. humoral fluids from unchallenged b. belcheri had lysozyme, antimicrobial, microbial agglutinating and haemagglutinating activities, which may represent part of the baseline level of innate immunity in this organism. after challenge with e. coli, the lysozyme activity, growth-inhibiting activiti ...200616352447
noradrenaline modulates the immunity of white shrimp litopenaeus vannamei.the total haemocyte count (thc), phenoloxidase activity, respiratory burst, superoxide dismutase (sod) activity, phagocytic activity and clearance efficiency in response to pathogen vibrio alginolyticus were measured when the white shrimp litopenaeus vannamei (18.4 +/- 1.2 g) were injected individually with noradrenaline at 10(-8), 10(-7) and 10(-6) mol shrimp(-1). for the shrimp that received noradrenaline at 10(-8), 10(-7) and 10(-6) mol shrimp(-1), the thc decreased by 15%, 21% and 32%, pheno ...200616377211
electron cryomicroscopic visualization of poma/b stator units of the sodium-driven flagellar motor in liposomes.a motor protein complex of the bacterial flagellum, poma/b from vibrio alginolyticus, was reconstituted into liposomes and visualized by electron cryomicroscopy. poma/b is a sodium channel, composed of two membrane proteins, poma and pomb, and converts ion flux to the rotation of the flagellar motor. escherichia coli and salmonella have a homolog called mota/b, which utilizes proton instead of sodium ion. pomb and motb have a peptidoglycan-binding motif in their c-terminal region, and therefore ...200616426637
regulation of polar flagellar number by the flhf and flhg genes in vibrio alginolyticus.the number and location of bacterial flagella vary with the species. the vibrio alginolyticus cell has a single polar flagellum, which is driven by sodium ions. we selected mutants on the basis of reduced swarming ability on soft agar plates. among them, we found two mutants with multiple polar flagella, and named them kk148 and nmb155. in pseudomonas species, it is known that flhf and flen, which are ftsy and mind homologs, respectively, are involved in regulation of flagellar placement and num ...200616428326
roles of charged residues of rotor and stator in flagellar rotation: comparative study using h+-driven and na+-driven motors in escherichia coli.in escherichia coli, rotation of the flagellar motor has been shown to depend upon electrostatic interactions between charged residues of the stator protein mota and the rotor protein flig. these charged residues are conserved in the na+-driven polar flagellum of vibrio alginolyticus, but mutational studies in v. alginolyticus suggested that they are relatively unimportant for motor rotation. the electrostatic interactions detected in e. coli therefore might not be a general feature of flagellar ...200616452430
white shrimp (litopenaeus vannamei) recombinant lysozyme has antibacterial activity against gram negative bacteria: vibrio alginolyticus, vibrio parahemolyticus and vibrio cholerae.c-type lysozyme has been described as an antibacterial component of the shrimp innate defence system. we determined quantitatively the antibacterial activity of white shrimp (litopenaeus vannamei) recombinant lysozyme against three gram negative bacteria: vibrio alginolyticus, vibrio parahemolyticus and vibrio cholerae, using a turbidimetric assay with live bacteria and differential bacterial viable count after interaction with the protein. in conclusion, the antibacterial activity of recombinan ...200616098764
vibrio alginolyticus infections in the netherlands after swimming in the north sea. 200617213549
a function of polar flagellum and anisotropic growth in vibrio alginolyticus early-phase colonies.we continuously observed growth of vibrio alginolyticus early-phase colonies on agar plates by phase-contrast microscopy. two mutants defective in motility on solid surfaces were used in this study: one (ym4) can swim in liquid environments using its polar flagellum, and the other (nmb198) cannot swim because it lacks any flagella. we found that isolated colonies of ym4 were generally more circular than those of nmb198. this observation suggests that ym4 cells moved slightly within a colony by t ...200616732454
effects of celiprolol on cardiovascular reactivity to laboratory stressors in patients with hypertension.an ideal antihypertensive drug should reduce blood pressure not only at rest but also during stressful situations. to test whether this criterion is fulfilled by celiprolol, a new beta 1-selective adrenergic blocker drug with peripheral vasodilating activity, we examined the effects of its oral administration on cardiovascular reactivity to laboratory stressors in 18 patients with essential hypertension, according to a placebo-controlled crossover design. in the 12 patients classified as "respon ...20061534042
diary from a week in practice. 20061595508
case report: myocardial ischemia: an overlooked substrate in syncope of aortic stenosis.the cause of the syncope in aortic stenosis has been the subject of controversy partly because only a few patients have been monitored during their syncopal episodes. among the mechanisms proposed are hypersensitive carotid sinus, complete a-v block, ventricular arrhythmias, and ischemic myocardial depression. it is now accepted that the syncope is caused by a vasodepressor response from stimulation of left ventricular baroceptors, resulting in reflex hypotension and bradycardia. this case repor ...20061539605
measurement of red blood cell antibodies in autoimmune hemolytic anemia.the enzyme-linked antiglobulin test (elat) was employed to measure the number of igg molecules per red blood cell (igg/rbc) in 11 patients with autoimmune hemolytic anemia (aiha). all patients with aiha had high levels of red cell-associated igg (110-3, 650 igg/rbc). the control group consisted of normal volunteers (n = 10) and patients with hereditary spherocytosis (n = 1), beta 0-thalassemia (n = 1), immunologic thrombocytopenic purpura (n = 3) and igg multiple myeloma (n = 4). all control ind ...20061797282
measurement of red blood cell antibodies in autoimmune hemolytic anemia.the enzyme-linked antiglobulin test (elat) was employed to measure the number of igg molecules per red blood cell (igg/rbc) in 11 patients with autoimmune hemolytic anemia (aiha). all patients with aiha had high levels of red cell-associated igg (110-3, 650 igg/rbc). the control group consisted of normal volunteers (n = 10) and patients with hereditary spherocytosis (n = 1), beta 0-thalassemia (n = 1), immunologic thrombocytopenic purpura (n = 3) and igg multiple myeloma (n = 4). all control ind ...20061797282
comparative study of magnetic resonance and ct scan imaging in cases of severe head injury.the distribution, frequency, and appearance of head injuries were evaluated with mri and ct in a prospective study of 155 patients with acute (n = 124) and chronic (n = 31) head injuries. mri was significantly more sensitive than ct in the detection of intraaxial injury at any stage. in severe cases, central structure lesions were detected in approximately 80% of patients. severity on admission was compatible with mr findings. however it was difficult to decide on neurobehavioural prognosis from ...20061414552
the use of potassium-titanyl-phosphate laser for laparoscopic removal of ovarian endometrioma.from january 1989 through january 1990, 31 patients thought to have ovarian endometrioma at ultrasonography were treated by operative laparoscopy and potassium-titanyl-phosphate laser. the endometriomas ranged from 2 to 9.2 cm in diameter by preoperative transvaginal ultrasonography. the operative procedure used was a three-puncture technique with a 600 micron fiber attached to a potassium-titanyl-phosphate laser. the power setting was 10 w with a continuous pulse. in all cases endometriomas wer ...20061828651
colorectal neoplasia: screening average risk individuals. 20061782367
colorectal neoplasia: screening average risk individuals. 20061782367
synthesis and duplex stability of oligonucleotides containing adenine-guanine analogues.the nucleosides n6-methoxy-2'-deoxyadenosine (dz) and 2-amino-9-(2-deoxy-beta-ribofuranosyl)-6-methoxyaminopurine (dk) have been synthesised and converted into 5'-o-dimethoxytrityl 3'-(2-cyanoethyl n,n-diisopropylphosphoramidites). these monomers have been used in machine dna synthesis to give a set of heptadecanucleotides containing up to three analogue nucleotides. the melting transitions (tm) show that the 17-mer duplexes containing z.t and z.c base-pairs have closely similar stabilities, as ...20061797376
synthesis and duplex stability of oligonucleotides containing adenine-guanine analogues.the nucleosides n6-methoxy-2'-deoxyadenosine (dz) and 2-amino-9-(2-deoxy-beta-ribofuranosyl)-6-methoxyaminopurine (dk) have been synthesised and converted into 5'-o-dimethoxytrityl 3'-(2-cyanoethyl n,n-diisopropylphosphoramidites). these monomers have been used in machine dna synthesis to give a set of heptadecanucleotides containing up to three analogue nucleotides. the melting transitions (tm) show that the 17-mer duplexes containing z.t and z.c base-pairs have closely similar stabilities, as ...20061797376
[excitation-contraction coupling and ion transport in membrane of skeletal muscle]. 20061698230
[cornea bank: an increase in the number of transplantations is possible]. 20061636297
diagnosis and management of the dandy-walker malformation: 30 years of experience.thirty-seven patients with dandy-walker malformation were treated between 1959 and 1989. eighty percent of patients became symptomatic by 3 years of age, with 70% presenting within the 1st year of life. hydrocephalus was present in 91% of patients at the time of diagnosis. clinical presentation was most often heralded by symptoms and signs of hydrocephalus with focal neurological findings being a less prominent feature. one third of children demonstrated developmental delay. associated congenita ...20061472430
how can the optometric profession expand the pool of qualified applicants?the health professions, including optometry, are facing a declining applicant pool. the american optometric association has taken steps to address the challenge of increasing the pool of qualified applicants to the schools and colleges of optometry. however, the most significant factor in the process will be the efforts of individual aoa members.20061447468
fine needle aspiration cytology of primary soft tissue tumors. morphologic analysis of the most frequent types.we reviewed 98 cases of fine needle aspiration of soft tissue tumors with histologic confirmation performed during an eight-year period and propose a working morphologic classification based on the most prominent cytologic features. six main tumor groups are recognized: myxoid, round cells, spindle cells, pleomorphic cells, polygonal cells and well differentiated. we believe that this classification allows recognition of the most common soft tissue tumors while helping with the differential diag ...20061449029
differentiated thyroid carcinomas: the relevance of various pathological features for tumour classification and prediction of tumour progress.in this series of 263 surgically treated cases of thyroid cancer, 12% were finally classified as benign lesions after histopathological review. difficulty in the assessment of tumour capsule invasion in follicular neoplasms was the most frequent cause of diagnostic error. squamous metaplasia, clusters of ground glass nuclei and psammoma bodies were found to be the most specific discriminators between papillary and follicular carcinomas. among papillary carcinomas, tumour diameter above 30 mm, th ...20061636246
biotechnology in japan--towards the year 2000 ... and beyond. 20061367243
hepatitis update. the delta virus. 20061539959
health checks on patients 75 years and over in nottinghamshire after the new gp contract.to investigate annual health checks for patients of 75 years and over required by the 1990 contract for general practitioners.20061393076
the measurement of radiant temperature in neonatal thermal environments.heat exchange through radiation is recognized as the dominant mode of heat transfer for an infant nursed in an incubator or warmer. a radiometer was constructed to measure the planar radiant temperature experienced by the infant. two heat-flow sensors of different emissivities were mounted onto a heat sink such that one measured principally convective heat exchange while the other measured convective and radiant heat exchange. the radiant heat exchange was obtained from the difference between th ...20061393209
bone marrow involvement by non-hodgkin's lymphoma: the clinical significance of morphologic discordance between the lymph node and bone marrow. nebraska lymphoma study group.bone marrow specimens from 317 patients with non-hodgkin's lymphoma (nhl) obtained at initial staging were evaluated for the presence of lymphoma or benign lymphoid aggregates. thirty-two percent (102 patients) had lymphoma in their bone marrow, and 9% had benign lymphoid aggregates. bone marrow lymphoma was present in 39% of low-grade, 36% of intermediate-grade, and 18% of high-grade lymphomas. the bone marrow was involved in 25% of patients with diffuse large-cell or immunoblastic nhl (ie, dif ...20061694234
fatal outcome with use of rectal morphine for postoperative pain control in an infant. 20061489382
ion channel expression by white matter glia: the type-1 astrocyte.we describe the electrophysiological properties of acutely isolated type-1 astrocytes using a new "tissue print" dissociation procedure. because the enzymes used did not destroy or modify the ion channels, and the cells retained many processes, the properties may reflect those in vivo. the types of ion channels in type-1 astrocytes changed rapidly during the first 10 postnatal days, when they attained their adult phenotype. this change was dependent on the presence of neurons. in culture, most o ...20061698397
cutaneous multilobated t-cell lymphoma with aggressive course.cutaneous multilobated t-cell lymphoma is an uncommon variant of skin-based peripheral t-cell lymphoma typically characterized by cutaneous nodules in elderly patients and a chronic clinical course. we report a case of the disease that led to the patient's death within 2 years after onset. this disease may be associated with a more aggressive clinical course than generally recognized.20071894770
[hyperoxalemia and secondary oxalosis of chronic renal insufficiency]. 20072074920
[a congenital esophagotracheal fistula in an adult]. 20072223222
[a congenital esophagotracheal fistula in an adult]. 20072223222
inbreeding in saguenay-lac-st-jean (quebec, canada): a study of catholic church dispensations 1842-1971.saguenay-lac-st-jean (slsj) is a rather geographically isolated region of quebec which shows a high occurrence of hereditary disorders. it has been suggested that high inbreeding might explain this situation. we studied the inbreeding in the slsj region by 10-year periods from 1842 till 1971 using the catholic church dispensations. the values of the mean inbreeding coefficient were found to be low during the whole period, reaching a peak of 22.94 x 10(-4) during the period 1902-1911. the values ...20071797631
acute intoxication as a cause of wild bird mortality in the netherlands from 1975-1989. 20071805755
[long-term results of the treatment of ruptured intracranial aneurysms. study of a series of 328 patients hospitalized from 1972 to 1984].between 1972 to 1984, 328 patients were admitted in the department of neurosurgery, for the treatment of a ruptured intracranial aneurysm, and being clinically in grades i to iv. these patients were submitted to a deferred surgery protocol. 5.5% of the patients died before surgery, and 94.5% were actually operated on. the follow-up was at least over one year, and up to 14 years (mean 3 years and 8 months). the long term result was appreciated according to the physical status of the patients and ...20073374722
a nonexponential, nonlogarithmic pharmacokinetic dosing method for gentamicin. 20071914430
terminal component of complement (c9) in the cerebrospinal fluid of patients with multiple sclerosis and neurologic controls.we measured the concentration of c9 in the csf and plasma of 93 consecutive patients referred for csf examination in an outpatient multispecialty clinic. we noted no differences in csf c9 or c9 index between patients with multiple sclerosis and neurologic controls.20072330118
[paraneoplastic syndromes in urology].the paraneoplastic syndromes in patients with malignant urological tumors are reviewed herein. some general aspects of these syndromes are briefly described and discussed. similarly, the different urological tumors in which paraneoplastic syndromes have been observed are briefly reviewed. these are very common in renal cancer and cancer of the prostate, uncommon in bladder cancer and rare in testicular cancer.20071796856
the immunostimulatory effects of sodium alginate and iota-carrageenan on orange-spotted grouper epinephelus coicoides and its resistance against vibrio alginolyticus.the lysozyme activity, alternative complement activity (ach50), respiratory burst, sod (superoxide dismutase) activity and phagocytic activity of orange-spotted grouper epinephelus coicoides were examined when the fish were injected intraperitoneally with sodium alginate at 10, 20, 30mgkg-1 and iota-carrageenan at 10, 20, 30mgkg-1, respectively after 24, 72 and 120 h. serum ach50 increased directly with dose after 24 and 72 h for both sodium alginate and iota-carrageenan treatments. the fish tha ...200716784873
genes encoding the vibrio harveyi haemolysin (vhh)/thermolabile haemolysin (tlh) are widespread in vibrios.v. harveyi vhh haemolysin, which shows high homology to the tlh haemolysin (the identities of their deduced amino acid sequences are up to 85.6%), is a putative virulence factor to marine cultured fish. a vhh probe, which is specific to v. harveyi vhha haemolysin gene, was used to screen ecor i digests of total dna from 57 vibrio strains, including 26 vibrio type strains, 20 v. harveyi isolates and 11 v. parahaemolyticus isolates. as a result, 1 strong hybridisation band was detected in 13 type ...200718062266
crystallization and preliminary x-ray analysis of moty, a stator component of the vibrio alginolyticus polar flagellar motor.the polar flagellum of vibrio alginolyticus is rotated by the sodium motor. the stator unit of the sodium motor consists of four different proteins: poma, pomb, motx and moty. motx and moty, which are unique components of the sodium motor, form the t-ring structure attached to the lp ring in the periplasmic space. moty has a putative peptidoglycan-binding motif in its c-terminal region and motx is suggested to interact with pomb. thus, motx and moty are thought to be required for incorporation a ...200717277446
the effect of dietary chitin supplementation on the survival and immune reactivity of the shore crab, carcinus maenas.adult male shore crabs (carcinus maenas) were maintained on a fish-based diet supplemented with 0, 5 or 10% chitin for 11 weeks. significantly greater mortality was found during this period in the control group (0% chitin) than those fed 10% chitin. crabs fed 5 or 10% chitin had lower numbers of cultivatable bacteria in the hepatopancreas than those on the basal diet alone. the addition of chitin had no significant effect on the serum concentrations of protein and glucose, and the levels of glyc ...200717289410
occurrence of vibrio spp. in blue mussels (mytilus edulis) from the german wadden sea.the present study aimed at determining the contamination of blue mussels grown in germany with relevant bacterial organisms. seasonal variations were examined. for that purpose, mussel samples were taken regularly between june 2004 and may 2005 in seven shellfish-growing areas of the german wadden sea. a total of 90 samples were analysed. the analysis included the compulsory microbiological parameters escherichia coli and salmonella spp., as well as the determination of the contamination with vi ...200717331605
immune responses and gene expression in white shrimp, litopenaeus vannamei, induced by lactobacillus plantarum.the total haemocyte counts, phenoloxidase (po) activity, respiratory bursts, superoxide dismutase (sod) activity, and phagocytic activity and clearance efficiency to vibrio alginolyticus, as well as prophenoloxidase (propo), lipopolysaccharide- and beta-1,3-glucan-binding protein (lgbp), serine protein (sp), and peroxinectin (pe) mrna transcription of l. vannamei, and its susceptibility to v. alginolyticus when the shrimp were fed diets containing lactobacillus plantarum at 0 (control), 10(7), a ...200717337209
expression, characterization and immunogenicity of a major outer membrane protein from vibrio alginolyticus.vibrio alginolyticus is one of the vibrio pathogens common to humans and marine animals. during infection and induction of the host immune response, outer membrane proteins of bacteria play an important role. in this study, an outer membrane protein gene (ompw) was cloned from v. alginolyticus and expressed in escherichia coli. the 645 bp open reading frame (orf) encodes a protein of 214 amino acid residues with a predicted molecular weight of 23.3 kda. the amino acid sequence showed a high iden ...200717342258
atp binding to the ktn/rck subunit ktra from the k+ -uptake system ktrab of vibrio alginolyticus: its role in the formation of the ktrab complex and its requirement in vivo.subunit ktra of the bacterial na(+)-dependent k(+)-translocating ktrab systems belongs to the ktn/rck family of regulatory proteins and protein domains. they are located at the cytoplasmic side of the cell membrane. by binding ligands they regulate the activity of a number of k(+) transporters and k(+) channels. to investigate the function of ktra from the bacterium vibrio alginolyticus (vaktra), the protein was overproduced in his-tagged form (his(10)-vaktra) and isolated by affinity chromatogr ...200717344221
the bidirectional polar and unidirectional lateral flagellar motors of vibrio alginolyticus are controlled by a single chey species.the bacterial flagellar motor is an elaborate molecular machine that converts ion-motive force into mechanical force (rotation). one of its remarkable features is its swift switching of the rotational direction or speed upon binding of the response regulator phospho-chey, which causes the changes in swimming that achieve chemotaxis. vibrio alginolyticus has dual flagellar systems: the na(+)-driven polar flagellum (pof) and the h(+)-driven lateral flagella (laf), which are used for swimming in li ...200717376072
growth response of vibrio cholerae and other vibrio spp. to cyanobacterial dissolved organic matter and temperature in brackish water.environmental control of growth and persistence of vibrios in aquatic environments is poorly understood even though members of the genus vibrio are globally important pathogens. to study how algal-derived organic matter and temperature influenced the abundance of different vibrio spp., baltic sea microcosms inoculated with vibrio cholerae, vibrio vulnificus, vibrio parahaemolyticus, vibrio alginolyticus and native bacterioplankton, were exposed to different temperatures (12-25 degrees c) and ame ...200717386033
characterization and resuscitation of viable but nonculturable vibrio alginolyticus vib283.the aim of this study was to investigate the viable but nonculturable (vbnc) state of the bacterium. vibrio alginolyticus vib283 was cultured in sterilized seawater microcosm at 4 degrees c. culturability of the cells in the microcosm was monitored by spread plate count (pc) on 2216e agar, pcs declined to undetectable levels (<0.1 cfu/ml) within 90 days. total cell counts remained constant throughout the period as determined by acridine orange direct count (aodc). the direct viable counts, on th ...200717492270
survival of vibrio alginolyticus in seawater and retention of virulence of its starved cells.the aim of this study was to evaluate the survival responses of four strains of vibrio alginolyticus in seawater under starvation conditions. we used microcosms containing sterilised seawater and incubated at ambient temperature (22-25 degrees c). v. alginolyticus maintained its culturability for at least nine months. long-term-starved cells showed an absence or a decrease in their enzymatic activities. resuscitation assays of viable but non-culturable (vbnc) cells were conducted and the recover ...200717524473
effect of the addition of four potential probiotic strains on the survival of pacific white shrimp (litopenaeus vannamei) following immersion challenge with vibrio parahaemolyticus.four bacterial strains isolated from the gastrointestinal tract of adult shrimp litopenaeus vannamei, vibrio alginolyticus utm 102, bacillus subtilis utm 126, roseobacter gallaeciensis slv03, and pseudomonas aestumarina slv22, were evaluated for potential use as probiotics for shrimp. in vitro studies demonstrated antagonism against the shrimp-pathogenic bacterium, vibrio parahaemolyticus ps-017. feeding shrimp with diets containing the potential probiotics showed the best feed conversion ratio ...200717544437
vibrios of the spotted rose snapper lutjanus guttatus steindachner, 1869 from northwestern mexico.to characterize and identify vibrios present in wild and cultured juvenile snappers (lutjanus guttatus) in northwestern mexico.200717578416
[taxonomic composition of bacteria associated with cultivated mollusks crassostrea lugubris and perna viridis and with the water of the gulf of nha trang lagoon, vietnam].one hundred and four strains of heterotrophic bacteria have been isolated and characterized from two species of bivalve mollusks cultivated in the gulf of nha trang (vietnam) and from the water of a mariculture farm. the isolates have been identified on the basis of morphological, physiological, biochemical, and chemotaxonomic properties, as well as by the content of g+c bases in dna. in the microflora of mollusks, vibrio alginolyticus was predominant; the pathogenic species v. harveyi and v. sp ...200717583223
comparison of four antibiotics with indigenous marine bacillus spp. in controlling pathogenic bacteria from shrimp and artemia.use of antibiotics for the control of bacterial diseases in shrimp culture has caused several adverse impacts to the industry. this has resulted in the search for alternative environment friendly approaches to overcome bacterial infections. this study was conducted to investigate the use of beneficial bacteria as an alternative to antibiotics. ten pathogenic bacterial species isolated from shrimp, penaeus monodon, and artemia cysts were tested for susceptibility to indigenous marine bacillus sub ...200717584435
isolation, sequencing and characterization of cluster genes involved in the biosynthesis and utilization of the siderophore of marine fish pathogen vibrio alginolyticus.in fish pathogen vibrio alginolyticus mvp01, the isolated 11-gene cluster consisted of two divergently transcribed, fe(3+) and ferric uptake regulator (fur) regulated operons, pvsabcde and psua-pvuabcde, sharing high similarity with that related to siderophore biosynthesis and transportation locus in v. parahaemolyticus. siderophore biosynthesis or utilization was blocked when pvsa and pvsd of the pvsabcde operon or pvua, pvub and pvue of the psua-pvuabcde operon was single-gene in-frame mutated ...200717593352
molecular cloning and expression analysis of interferon-gamma inducible lysosomal thiol reductase (gilt)-like cdna from disk abalone (haliotis discus discus).interferon gamma (ifn-gamma) inducible lysosomal thiol reductase (gilt) has been described as a key enzyme in processing and presentation of major histocompatibility complex (mhc) class ii restricted antigen (ag) by catalyzing disulfide bond (s-s) reduction in mammals. abalone gilt-like (abgilt) full-length cdna was isolated from the normalized disk abalone cdna library. the 807-bp abgilt cdna consists of an open reading frame of 684-bp, encoding 228 amino acid residues. the predicted abgilt pro ...200717599346
rapid and specific identification of 5 human pathogenic vibrio species by multiplex polymerase chain reaction targeted to dnaj gene.a multiplex polymerase chain reaction (pcr) method, specifically designed for application in routine diagnostic laboratories, was developed for identifying 5 human pathogen vibrio species: vibrio cholerae, vibrio parahaemolyticus, vibrio vulnificus, vibrio mimicus, and vibrio alginolyticus. this assay directed toward the dnaj gene was tested on a total of 355 strains representing 13 vibrio species and 17 non-vibrio species. specific pcr fragments were produced in isolates belonging to the 5 targ ...200717614235
research and characterization of pathogenic vibrios from bathing water along the conero riviera (central italy).the occurrence and pathogenicity of vibrios in bathing water were investigated along the conero riviera (adriatic sea, central italy). vibrio spp. enumeration was performed on thiosulfate-citrate-bile-salts-sucrose-agar by the membrane filter method, and identification was done through a biochemical protocol. all isolates were tested for the presence of cytotoxicity, protease, lipase, elastase, gelatinase, urease, haemolytic activity, ctx, tdh and trh genes by conventional methods. in all, 200 v ...200717619048
cloning and expression of the gene encoding an extracellular alkaline serine protease from vibrio alginolyticus strain hy9901, the causative agent of vibriosis in lutjanus erythopterus (bloch).a 750-bp internal fragment of the alkaline serine protease gene (asp) from the vibrio alginolyticus strain hy9901 was amplified by polymerase chain reaction (pcr). the flanking sequences of the 5'- and 3'- ends of the asp gene were characterized by reverse and nested pcr. sequence analysis showed that the asp gene contained an 1893-bp orf encoding 630 amino acids. the deduced amino acid sequence of the asp (alkaline serine protease) precursor showed significant homology with several bacterial al ...200717640252
cloning and expression of gene encoding the thermostable direct hemolysin from vibrio alginolyticus strain hy9901, the causative agent of vibriosis of crimson snapper (lutjanus erythopterus).the main aims of this study were to clone and express complete open reading frame (orf) of thermostable direct haemolysin gene (tdh) from vibrio alginolyticus strain hy9901 in escherichia coli, and further evaluate the virulence of expressed tdh on mouse and crimson snapper.200717650188
[pathogenic vibrios in oysters (crassostrea rhizophorae) served at restaurants in rio de janeiro: a public health warning].forty oyster samples (crassostrea rhizophorae) served raw in 15 restaurants in the city of rio de janeiro were evaluated in order to investigate the presence of vibrio spp. the oyster samples were analyzed and subjected to enrichment in alkaline peptone water with the addition of 1 and 3% nacl and incubated at 37 degrees c for 24 hours. following this, the cultures were seeded onto thiosulfate citrate bile sucrose agar (tcbs) and the suspected colonies were subjected to biochemical characterizat ...200717653465
a secretory leukocyte proteinase inhibitor (slpi)-like protein from litopenaeus vannamei haemocytes.a partial clone coding for a two-wap domain protein was isolated from a litopenaeus vannamei haemocytes cdna library. the complete sequence was obtained by race, and the full-length cdna sequence is 0.8 kb long and encodes for a 116-amino acid protein. the domain composition is similar to the mammalian wfdc5 (wap four disulfide core) and secretory leukocyte proteinase inhibitor (slpi). modifications in expression were determined by real-time pcr, after injection of vibrio alginolyticus, suggesti ...200717664073
identification of a novel endochitinase from a marine bacterium vibrio proteolyticus strain no. 442.chitin binding proteins prepared from vibrio proteolyticus were purified and the n-terminal amino-acid sequence of a protein from a 110-kda band on sds-page was found to be 85-90% identical to the 22nd-41st residues of the n-termini of chitinase a precursor proteins from other vibrios. we cloned the corresponding gene, which encodes a putative protein of 850 amino acids containing a 26-residue signal sequence. the chitinase precursor from v. proteolyticus was 78-80% identical to those from vibri ...200717690022
characterization of the vibrio alginolyticusfur gene and localization of essential amino acid sites in fur by site-directed mutagenesis.the expression of iron-regulated genes in bacteria is typically controlled by the ferric uptake regulator (fur) protein, a global transcriptional repressor that regulates functions as diverse as iron acquisition, oxidative stress, virulence and acid tolerance. we have identified a fur homologue in vibrio alginolyticus and shown that it complements an escherichia coli fur mutant. reverse transcriptase pcr (rt-pcr) analysis proved that unlike many other fur homologues, v. alginolyticusfur is not u ...200717693709
immune stimulation and improved infection resistance in european sea bass (dicentrarchus labrax) fed mannan oligosaccharides.the objective of this study was to determine the effect of two levels of inclusion of mannan oligosaccharides derived from the outer cell wall of a select strain of saccharomyces cerevisiae (bio-mos, alltech inc, usa) on growth, feed utilization, immune status and disease resistance of european sea bass (dicentrarchus labrax). specimens of 35 g at initial density of 3 kg/m3 were fed during 67 days at 0 per thousand, 2 per thousand and 4 per thousand dietary mos level of inclusion in a commercial ...200717766145
molecular characterization of goose-type lysozyme homologue of large yellow croaker and its involvement in immune response induced by trivalent bacterial vaccine as an acute-phase protein.lysozyme acts as an innate immunity molecule against the invasion of bacterial pathogens. here, the cdna of a goose-type lysozyme (g-lysozyme) was cloned from large yellow croaker (pseudosciana crocea) by expressed sequence tags (est) and race-pcr techniques. the full-length cdna of large yellow croaker g-lysozyme (lycgl) is 716 nucleotides (nt) encoding a protein of 193 amino acids (aa), with a theoretical molecular weight of 21.3kda. the deduced lycgl possessed the typical structural features ...200717850883
a novel multiplex pcr for the identification of vibrio parahaemolyticus, vibrio cholerae and vibrio vulnificus.to establish a simple multiplex polymerase chain reaction (pcr) that will identify vibrio parahaemolyticus, vibrio cholerae and vibrio vulnificus.200717897378
expression and immunogenicity analysis of two iron-regulated outer membrane proteins of vibrio parahaemolyticus.genes of two iron-regulated outer membrane proteins of vibrio parahaemolyticus zj2003, a pathogenic strain isolated from large yellow croaker (pseudosciaena crocea), psua and pvua, were cloned and expressed as n-terminal his(6)-tagged proteins in escherichia coli bl(21)(de(3)). the recombinant fusion proteins were purified with nickel chelate affinity chromatography. to analyze the immunogenicity of the proteins, groups of large yellow croaker were immunized with the purified recombinant psua, p ...200717928925
luxo controls extracellular protease, haemolytic activities and siderophore production in fish pathogen vibrio alginolyticus.to characterize the luxo gene in fish pathogen vibrio alginolyticus mvp01 and investigate its roles in regulation of extracellular products (ecp) and siderophore production.200717953563
development of a sybr green i real-time pcr for quantitative detection of vibrio alginolyticus in seawater and seafood.vibrio alginolyticus is an economically important micro-organism. the main aim of the present study was to develop a real-time polymerase chain reaction (pcr) assay for rapid, sensitive and effective quantification of v. alginolyticus in seawater and seafood.200717953599
oil-utilizing bacteria associated with fish from the arabian gulf.the objectives were to count and identify the oil-utilizing bacteria associated with fish, and to study their hydrocarbon-degradation potential.200717953689
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