Publications

TitleAbstractYear
Filter
PMID
Filter
cyclic fluctuations in fasting serum bile acid levels detected with a sensitive enzyme/bioluminescent assay.a sensitive two-step bioluminescent assay for total serum bile acids was developed using commercially available enzymes. in the first step, the bile acids present in 10 microl of alkali-treated serum were oxidised at ph 9.5 by high purity 3 alpha-hydroxysteroid dehydrogenase to form nadh. then, nadh was quantitated at ph 6.5 under optimal conditions for bioluminescence using fmn:nadh oxidoreductase and luciferase from photobacterium fischeri. the enzyme/bioluminescent assay correlated well with ...19873480084
on the function of aldehyde in bacterial bioluminescence: evidence for an aldehyde requirement during luminescence from the frozen state. 19685666729
the effect of anaesthetic agents on the emission of light by luminous bacteria. 19695774528
bioluminescence as an in vivo index of radiation damage. 19695777118
tryptophanase in diverse bacterial species.the distribution of tryptophanase was studied. the highest observed specific activity, mumoles per minute per milligram (dry weight) cells, is given in parentheses after each species. tryptophanase was inducible and repressible in escherichia coli (.914), paracolobactrum coliforme (.210), proteus vulgaris (.146), aeromonas liquefaciens (.030), photobacterium harveyi (.035), sphaerophorus varius (.021), bacteroides sp. (.048), and corynebacterium acnes (.042). the enzyme was constitutive and nonr ...19695781572
measuring the effect of air pollutants on bacterial luminescence: a simplified procedure. 19695797990
the polarity of proton translocation in some photosynthetic microorganisms. 19695802881
nutrition and metabolism of marine bacteria. xv. relation of na+-activated transport to the na+ requirement of a marine pseudomonad for growth.drapeau, gabriel r., (mcgill university, montreal, quebec, canada), tibor i. matula, and robert a. macleod. nutrition and metabolism of marine bacteria. xv. relation of na(+)-activated transport to the na(+) requirement of a marine pseudomonad for growth. j. bacteriol. 92:63-71. 1966.-a marine pseudomonad was found to require 50 to 100 mm na(+) for maximal rate of oxidation of d-galactose and for the transport of d-fucose-h(3) into the cells. the same organism required 150 to 200 mm na(+) for th ...19665941284
[species composition and some properties of marine periphyton bacteria isolated from species-specifically stained surfaces]. 19666002796
the role of oxygen in the photoexcited luminescence of bacterial luciferase. 19676017740
a comparative study of carbon source requirements of mycobacterium avium, group ii scotochromogens, group 3 nonphotochromogens, and mycobacterium terrae in the presence of glutamate-nitrogen. 19676039105
an analysis of the relations between fluorescence and photochemistry during photosynthesis. 19676040858
the primary structure of iron-superoxide dismutase from photobacterium leiognathi.the complete amino acid sequence of iron-superoxide dismutase from photobacterium leiognathi was determined. the sequence was deduced following characterization of the peptides obtained from tryptic, chymotryptic, and staphylococcus aureus v-8 protease digests of the apoprotein. the amino acid sequence listed below is made up of 193 residues. it is the first complete sequence to be determined for an iron-superoxide dismutase. the iron-superoxide dismutase shows the same order of homology with th ...19873542995
generation of fatty acids by an acyl esterase in the bioluminescent system of photobacterium phosphoreum.the fatty acid reductase complex from photobacterium phosphoreum has been discovered to have a long chain ester hydrolase activity associated with the 34k protein component of the complex. this protein has been resolved from the other components (50k and 58k) of the fatty acid reductase complex with a purity of greater than 95% and found to catalyze the transfer of acyl groups from acyl-coa primarily to thiol acceptors with a low level of transfer to glycerol and water. addition of the 50k prote ...19846147345
[cloning and expression of genes of the luminescence system in photobacterium leiognathi].the genes of photobacterium leiognathi luminescence system were cloned in plasmid puc18. escherichia coli cells harboring a recombinant plasmid pphl1 are luminescent. pphl1 contains luciferase genes and genes responsible for aldehyde biosynthesis. the luminescence of escherichia coli is subject to autoinductor regulation similar to the one existing in luminescent bacteria. the 2.7 kb fragment of photobacterium leiognathi dna containing the genes for alpha- and beta-luciferase subunits were clone ...19873683427
the ribonucleotide sequence of 5s rrna from two strains of deep-sea barophilic bacteria.deep-sea bacteria were isolated from the digestive tract of animals inhabiting depths of 5900 m in the puerto rico trench and 4300 m near the walvis ridge. growth of two bacterial strains was measured in marine broth and in solid media under a range of pressures and temperatures. both strains were barophilic at 2 degrees c (+/- 1 degrees c) with an optimal growth rate of 0.22 h-1 at a pressure 30% lower than that encountered in situ. at 1 atm they grew at temperatures ranging from 1.2 to 18.2 de ...19846206199
relationship between luminous fish and symbiosis. ii. chemical composition of lipopolysaccharides isolated from symbiotic luminous bacteria in the luminous marine fish, physiculus japonicus. 19863702775
superoxide dismutases.superoxide dismutases (ec 1.15.1.1) are metalloenzymes that catalytically scavenge the superoxide radical. they are essential for the aerobic survival of all forms of life. there are three types of superoxide dismutase, containing manganese, iron, or copper and zinc. the copper--zinc type has generally been isolated from eukaryotic cells except for the enzyme for the symbiotic marine bacterium photobacterium leiognathi. the copper--zinc type, from different sources, has a molecular weight of abo ...19806258885
comparative biochemistry of the cell envelopes of photobacterium leiognathi and escherichia coli.photobacterium leiognathi closely resembles escherichia coli with respect to cell lysis by lysozyme, and the fractionation of outer and cytoplasmic membranes. the two organisms differ in their phospholipid contents and, more significantly, in outer membrane protein compositions.19836352861
multiple regression analysis of toxic interactions: application to the microtox test and general comments. 19863708174
comparison of the microtox test with the 96-hr lc50 test for the harpacticoid nitocra spinipes.a comparison between the static 96-hr lc50 test with the brackish water harpacticoid nitocra spinipes and the microtox (beckman instruments, inc.) screening method has been done. the relationship between the two bioassays were evaluated for 16 pure and technical chemicals and 11 complex effluents from different types of industries. the correlation between the 96-hr lc50 values for nitocra and the 5-, 15-, and 30-min effective concentration (ec50) for pure and technical chemicals had r2 values ra ...19863709402
intersubunit transfer of fatty acyl groups during fatty acid reduction.fatty acid reduction in photobacterium phosphoreum is catalyzed in a coupled reaction by two enzymes: acyl-protein synthetase, which activates fatty acids (+atp), and a reductase, which reduces activated fatty acids (+nadph) to aldehyde. although the synthetase and reductase can be acylated with fatty acid (+atp) and acyl-coa, respectively, evidence for acyl transfer between these proteins has not yet been obtained. experimental conditions have now been developed to increase significantly (5-30- ...19863782102
[metabolic organization of the luminescent system of phosphorescent bacteria]. 19836418498
nucleotide base sequence of vibrionaceae 5 s rrna.nucleotide base sequences of 5 s rrnas isolated from vibrio vulnificus, vibrio anguillarum, and aeromonas hydrophila were determined. comparisons among these and sequences of 5 s rrnas from other species of vibrionaceae provide information useful in the evaluation of the evolution of bacterial species.19846479333
the amino-acid sequence of copper/zinc superoxide dismutase from swordfish liver. comparison of copper/zinc superoxide dismutase sequences.the amino acid sequence of copper/zinc superoxide dismutase from swordfish (xiphias gladius) liver has been determined by alignment of the tryptic peptides according to the known sequence of bovine erythrocyte copper/zinc superoxide dismutase. this alignment has resulted in the ligands to the copper (his-47, 49, 76 and 94) and the zinc (his-76, 85, 134 and asp-97) being conserved in all the copper/zinc superoxide dismutases sequenced so far. also conserved in the sequences are the cysteines form ...19846510412
evaluation of a new approach to the safety assessment of biomaterials.the effectiveness of a bacterial luminescence inhibition assay in assessing the toxicity of compounds which are released from biomaterials was evaluated. luminescence from a strain of bacteria most closely resembling photobacterium phosphoreum was measured. the concentration that inhibited luminescence by 50% (ec50) was determined for selected plasticizers, monomers and additives. the intraperitoneal (i.p.-ald) and intravenous (i.v.-ald) approximate lethal doses were determined using mice. by ra ...19863816615
spectroscopic investigations of the single tryptophan residue and of riboflavin and 7-oxolumazine bound to lumazine apoprotein from photobacterium leiognathi.spectroscopic techniques have been applied to investigate the conformation, local structure, and dynamic properties of the apoprotein of the lumazine protein from photobacterium leiognathi and the holoprotein reconstituted with either the natural ligand 6,7-dimethyl-8-ribityllumazine or the closely related analogues riboflavin and 6-methyl-7-oxo-8-ribityllumazine (7-oxolumazine). the analogues are bound similarly to the natural prosthetic group. they exhibit similar shifts on binding in their ab ...19873828324
relationship between ion requirements for respiration and membrane transport in a marine bacterium.intact cells of the marine bacterium alteromonas haloplanktis 214 oxidized nadh, added to the suspending medium, by a process which was stimulated by na+ or li+ but not k+. toluene-treated cells oxidized nadh at three times the rate of untreated cells by a mechanism activated by na+ but not by li+ or k+. in the latter reaction, k+ spared the requirement for na+. intact cells of a. haloplanktis oxidized ethanol by a mechanism stimulated by either na+ or li+. the uptake of alpha-aminoisobutyric ac ...19846690427
[action of phenobarbital on bacterial luciferase of photobacterium fischeri].the effect of phenobarbital, a typical substrate of monooxygenases from higher organisms, on bioluminescence of the marine bacterium photobacterium fischeri and bacterial luciferase was studied. phenobarbital was shown to be an effective quenching agent owing to the interaction with cytochrome p-450, a terminal luciferase component. a competitive interrelation was found between phenobarbital and an aliphatic aldehyde, the substrate of the luminescent reaction.19854058324
structure and arrangement of flagella in species of the genus beneckea and photobacterium fischeri.species of marine bacteria belonging to the genus beneckea and strains of photobacterium fischeri were negatively stained and examined by means of the electron microscope to determine the structure and arrangement of their flagella. all of the species of the genus beneckea had single, polar, sheathed flagella when grown in liquid medium. when grown on solid medium, most strains of b. campbellii and b. neptuna and all strains of b. alginolytica and b. parahaemolytica had unsheathed, peritrichous ...19714105030
catabolite repression of bacterial bioluminescence: functional implications.the synthesis of the bioluminescent system of the marine luminous bacterium photobacterium fischeri (strain mav) is subject to both transient and catabolite repression by glucose, and this repression can be reversed by adenosine 3':5'-cyclic monophosphate. catabolite repression is a mechanism that characteristically controls the synthesis of inducible enzymes involved in energy metabolism. the fact that luciferase synthesis is subject to this control suggests that whatever its role(s) may be, it ...19724338581
growth and luminescence of luminous bacteria on modified haneda's medium. 19846727711
the aldehyde content of luminous bacteria and of an "aldehydeless" dark mutant.fatty aldehydes, present in the luminescent cells of photobacterium phosphoreum and achromobacter fischeri, and to a very slight extent in the cells of a visually dark, "aldehydeless" mutant of the latter species, were extracted, purified, and oxidized to the corresponding acids. the acids were analyzed by mass spectrometry. the results, in conjunction with various other lines of evidence, indicate that saturated fatty aldehydes, comprising mostly dodecanal, tetradecanal, and hexadecanal, functi ...19744531008
a new rapid and sensitive bioluminescence assay for antibiotics that inhibit protein synthesis.a new sensitive, rapid and simple bioluminescence assay for antibiotics inhibiting protein synthesis is described. in this assay the ability of the tested antibiotic to inhibit the de novo synthesis of the enzymes participating in the bacterial luminescence system is determined by means of a dark variant of a luminous bacterium that undergoes prompt induction of the luminescence system with certain dna-intercalating agents. upon induction, the in vivo luminescence of the dark variant is increase ...19846746464
chemical and biological properties of lipopolysaccharide from a marine bacterium, photobacterium phosphoreum pj-1.the chemical and biological properties of the lipopolysaccharide (lps) isolated from a marine bacterium, photobacterium phosphoreum pj-1, were studied. this lps consists of 40.6% carbohydrate, 27.3% fatty acid, 0.2% 2-keto-3-deoxyoctonate (kdo) and other components. one characteristic of this lps is its small amount of kdo, the basic component of the usual lps. electrophoresis in sodium dodecylsulfate polyacrylamide gel revealed at least two staining bands for carbohydrates. these bands were con ...19826752664
[use of the bio- and chemiluminescence in a clinical laboratory]. 19816752924
evidence for a natural gene transfer from the ponyfish to its bioluminescent bacterial symbiont photobacter leiognathi. the close relationship between bacteriocuprein and the copper-zinc superoxide dismutase of teleost fishes. 19816787049
structurally distinct bacterial luciferases. 19695365778
immunochemical comparisons among lipopolysaccharides from symbiotic luminous bacteria isolated from several luminous marine animals. 19826820471
raman spectra of flavin bound in flavodoxins and in other flavoproteins. evidence for structural variations in the flavin-binding region.the resonance coherent anti-stokes raman scattering (cars) spectra for a number of flavoproteins are found to be fingerprints for the particular type of flavoprotein. one group studied were the bacterial flavodoxins: desulfovibrio vulgaris, desulfovibrio desulfuricans, azotobacter vinelandii, megasphaera elsdenii, clostridium kluyverii and clostridium formicoaceticum. the other examples were the enzymes lactate monooxygenase and glucose oxidase. fmn complexed to vibrio harveyi luciferase, and a ...19836840072
saturation behavior of the manganese-containing superoxide dismutase from paracoccus denitrificans.a pulse radiolysis study of the mn-superoxide dismutase from paracoccus denitrificans has shown that, at concentration of 0(2)-. below 0.8 x 10(-4)m, the catalyzed dismutation of 0(2)-. is a first order reaction with regard to 0(2)-.. at concentration of 0(2)-. above 0.8 x 10(-4)m, the mn-superoxide dismutase is shown to catalyze superoxide dismutation with a mechanism which exhibits saturation kinetics. this behavior was previously found in the bovine cu/zn-superoxide dismutase and in the fe-su ...19836860328
phagocytosis-induced mutagenesis in bacteria.dark mutants of the luminous bacteria photobacterium fischeri reverted to hereditary stable luminescent forms when incubated with human polymorphonuclear neutrophils (pmn). the maximal mutagenic effect occurred during the first 15 min of phagocytosis, and was dependent on the phagocyte:bacterium ratio as well as on the integrity of the pmn cells. heat-killed phagocytes or disintegrated phagocytes did not show any mutagenic activity, whereas the supernatant of the phagocytosis reaction exerted mu ...19836866004
the effect of radiation on bioluminescent bacteria: possible use of luminescent bacteria as a biological dosemeter. 19836867115
bioluminescence and radiation response to photobacterium fischeri h-2. 19705429845
cellular control of the synthesis and activity of the bacterial luminescent system.in bioluminescent bacteria growing in shake flasks, the enzyme luciferase has been shown to be synthesized in a relatively short burst during the period of exponential growth. the luciferase gene appears to be completely inactive in a freshly inoculated culture; the pulse of preferential luciferase synthesis which occurs later is the consequence of its activation at the level of deoxyribonucleic acid transcription which is attributed to an effect of a "conditioning" of the medium by the growing ...19705473898
[bioluminescence study of nad-dependent dehydrogenase and isoenzyme activity of human blood using soluble and immobilized bacterial luciferase]. 19836884193
the primary structure of cu-zn superoxide dismutase from photobacterium leiognathi: evidence for a separate evolution of cu-zn superoxide dismutase in bacteria.the complete amino-acid sequence of the copper-zinc superoxide dismutase of the photobacterium leiognathi was determined. the fragmentation strategy employed included cyanogen bromide cleavage at its methionine residues and the only tryptophan residue. the s-carboxymethylated chain was further cleaved by means of trypsin, in order to obtain overlapping fragments. for sequence determination automated solid or liquid-phase techniques of edman degradation were used. c-terminal amino acids of the en ...19836884993
[study on a photobacterium isolated from the light organ of the leiognathidae fish]. 19675624740
aspects of light production by photobacterium fischeri.studies of luminescence in growing cultures of photobacterium fischeri revealed the characteristic kinetics of light emission, including a minimal phase of bacterial light output. a dialyzable substance present in the nutrient broth medium caused this transient inhibition in light production, although this substance did not affect culture growth. experiments were carried out to determine the mechanism of action and the chemical properties of the inhibitor. the results suggest that the inhibitor ...19685643069
the effects of phosphate on the structure and stability of the luciferases from beneckea harveyi, photobacterium fischeri, and photobacterium phosphoreum. 19806967319
complementation of subunits from different bacterial luciferases. evidence for the role of the beta subunit in the bioluminescent mechanism.complementation of the nonidentical subunits (alpha and beta) of luciferases isolated from two different bioluminescent strains, beneckea harveyi and photobacterium phosphoreum, has resulted in the formation of a functional hybrid luciferase (alpha h beta p) containing the alpha subunit from b. harveyi luciferase (alpha h) and the beta subunit from p. phosphoreum luciferase (beta p). the complementation was unidirectional; activity could not be restored by complementing the alpha subunit of p. p ...19806969259
taxonomy and description of vibrio fluvialis sp. nov. (synonym group f vibrios, group ef6). 19816971864
[bioluminescence and its analytic applications]. 19836146159
proteolytic inactivation of luciferases from three species of luminous marine bacteria, beneckea harveyi, photobacterium fischeri, and photobacterium phosphoreum: evidence of a conserved structural feature.upon limited proteolysis of luciferases from the luminous marine bacteria photobacterium fischeri, photobacterium phosphoreum, and beneckea harveyi, the rate of loss of luciferase activity is the same as the rate of loss of the heavier subunit of all three enzymes. it thus appears that the larger subunit of the luciferase from p. phosphoreum should be designated alpha based on its apparent homology with the alpha subunits of the luciferases from b. harveyi and p. fischeri. the luciferase from b. ...19806161366
[effect of camp on the growth and development of luminescence in photobacterium belozerskii].the effect of camp on the parameters characterizing the development of luminescence in photobacterium belozerskii is discussed. an addition of camp to the culture medium shortened the latent time of luminescence development by 3--6 hours. the intensity of bacterial luminescence increased with a varying rate. during luminescence enhancement the rate of luciferase synthesis increased by a factor of 10 to 10(3). the rate of luciferase synthesis and the maxiumum level of bacterial luminescence when ...19806253982
a new, sensitive and simple bioluminescence test for mutagenic compounds.a spontaneous dark variant of the luminous bacterium photobacterium leiognathi was isolated. the reversion frequency of this variant to genetic-hereditary luminescent cells is greatly increased by nanogram quantities of different base-substitution and frameshift agents. this makes it possible to detect mutagenic compounds at concentrations 100 times lower than that detected by the ames test. curing agents, such as acridine dyes, ethidium bromide and sodium dodecyl sulfate, are also very active i ...19806990233
copper-zinc superoxide dismutase from caulobacter crescentus cb15. a novel bacteriocuprein form of the enzyme.a bacteriocuprein is a copper- and zinc-containing superoxide dismutase isolated from a bacterium. until recently, the first and only documented bacteriocuprein was that from the marine bacterium photobacterium leiognathi, which lives symbiotically with leiognathid fishes. a new bacteriocuprein has been discovered, purified, and characterized from the free living, non-symbiotic bacterium, caulobacter crescentus cb15. in its native molecular weight, homodimeric subunit structure, specific activit ...19827050107
isolation and characterization of a protein with cyanide-sensitive superoxide dismutase activity from the prokaryote, paracoccus denitrificans.1. a protein with cyanide-sensitive superoxide dismutase activity was isolated from the prokaryote paracoccus denitrificans. 2. this enzyme, present in low amount in the cell, represented not more than 10% of the total cellular superoxide dismutase activity. it was obtained in a form which was 20-40-times less active than the main superoxide dismutase of p. denitrificans which is a manganese-containing enzyme. 3. it was a soluble monomeric enzyme, highly negatively charged (pi = 4.8), with an ap ...19827066332
superoxide dismutases: exploration of apparent anomalies: a gene transfer and a functional replacement. 19827067913
[continuous cultivation of photobacterium phosphoreum luminescent bacteria with control of the luminescence].the paper describes a procedure for continuous cultivation of luminescent bacteria photobacterium phosphoreum according to which the nutrient flow is controlled with respect to the luminescence intensity. the biomass yield of this cultivation procedure at the given luminescence intensity 6.1 x 10(12) quantum x ml-1 s-1 is over three times higher than that obtained in periodic cultivation, with the specific cell luminescence being identical in both cases. the cultivation process is unstable at th ...19836353408
evolutionary relationships in vibrio and photobacterium: a basis for a natural classification. 19836357056
association between lumazine protein and bacterial luciferase: direct demonstration from the decay of the lumazine emission anisotropy. 19827093241
diluent composition for use of api 20e in characterizing marine and estuarine bacteria.nine chemically defined inoculation diluents, with compositions ranging from 0.85% nacl to 35% marine salts, were used to evaluate the influence of diluent composition on the biochemical profiles of 30 marine and estuarine bacterial strains, including species of vibrio, aeromonas, allomonas, and photobacterium. results demonstrated that a 20% marine salts diluent enabled the characterization of halophilic strains normally nonreactive by the api 20e system. furthermore, the use of 20% marine salt ...19827125655
[thermoinactivation of bacterial luciferase].the kinetics of thermal inactivation of bacterial luciferase was studied. it was found that the reaction substrates produce a weak protecting effect against the enzyme inactivation. edta, dithiothreitol and bovine serum albumin considerably stabilize the luciferase. the hypothetical mechanism of the enzyme inactivation involves oxidation of sh-groups and dissociation of the dimer into inactive monomers.19827150668
continuous-flow determination of primary bile acids, by bioluminescence, with use of nylon-immobilized bacterial enzymes.we describe a continuous-flow bioluminescence method for measuring primary bile acid in serum, with nylon as the solid support. 7 alpha-hydroxysteroid dehydrogenase (ec 1.1.1. 159), a bacterial luciferase (ec 1.14.14.3), and nad+:fmn oxido-reductase (ec 1.6.8.1) are covalently co-immobilized on a nylon coil (1 m x 1.0 mm i.d.). the assay is highly specific for 7 alpha-hydroxy bile acids. other bile acids and steroids do not interfere. the continuous-flow light-emitting system, in which the react ...19846362913
occurrence of uronic acid in lipopolysaccharides of vibrionaceae.the occurrence of uronic acid as a sugar constituent of lipopolysaccharides (lps) in vibrionaceae was demonstrated for the first time. more than 100 strains were examined. of five genera constituting vibrionaceae, i.e., vibrio, aeromonas, plesiomonas, photobacterium, and lucibacterium, the latter three contained uronic acid in lps of all of their constituting members examined, while it was totally lacking in aeromonas lps so far tested. only the members of genus vibrio were found to be divided i ...19827169971
sugar composition of lipopolysaccharides of family vibrionaceae. absence of 2-keto-3-deoxyoctonate (kdo) except in vibrio parahaemolyticus o6. 19827176968
bacterial bioluminescence as a bioassay for mycotoxins.the use of bacterial bioluminescence as a toxicological assay for mycotoxins was tested with rubratoxin b, zearalenone, penicillic acid, citrinin, ochratoxin a, pr-toxin, aflatoxin b1, and patulin. the concentrations of mycotoxins causing 50% light reduction (ec50) in photobacterium phosphoreum were determined immediately and at 5 h after reconstitution of the bacteria from a freeze-dried state. generally, less toxins were required to obtain an ec50 at 5 h. the effects of the above mycotoxins on ...19827181501
[fatty acid makeup changes in the luminescent bacteria, photobacterium mandapamensis, in periodic cultivation].the fatty acid composition of the luminescent bacterium photobacterium mandapamensis and its spontaneous dark mutant was studied in dynamics. lipids of the both strains extracted with a methanol -- chloroform mixture contained the following fatty acids: lauric, tridecanoic, myristic, tetradecenic, pentadecanoic, pentadecenic, palmitic, palmitoleic, heptadecanoic, c17-cyclopropanoic, stearic, octadecenic, and nonadecanoic. the content of palmitoleic acid was the highest (57% of the total). not al ...19807207260
[synthetic medium for photobacterium mandapamensis luminescent bacteria]. 19807207266
extracellular lumazine from aggregating dictyostelium discoideum cells. influence of ph on its fluorescence.lumazine has been demonstrated to be one of the two main compounds responsible for the extracellular fluorescence linked with the aggregation ability of dictyostelium discoideum, strain ax-2. the other compound, also having lumazine properties, is, however, different from the 7-hydroxylumazine proposed previously. the influence of ph on the fluorescence of lumazine was studied. the possible use of extracellular pteridines as ph markers was stressed and a method for the determination of the amoun ...19846519644
[lactate dehydrogenase activity and its isoenzyme in a system coupled with bacterial luciferase].properties of a coupled system "ldh-bacterial luciferase" were studied. the light-generating enzymatic system from luminescent bacteria photobacterium fisheri was used as an indicator of the dehydrogenase activity. kinetic parameters of the coupled system were studied using commercially available preparation of ldh and the enzyme from human blood plasma. the luminescent activity of bacterial preparation was shown to correlate with the activity of ldh and its isoenzymes within wide range of blood ...19846528536
[bacterial luciferase in reactions with catalytically reduced flavin mononucleotide]. 19817225439
differential acylation in vitro with tetradecanoyl coenzyme a and tetradecanoic acid (+atp) of three polypeptides shown to have induced synthesis in photobacterium phosphoreum.acylation of extracts of photobacterium phosphoreum at different stages of growth with [3h]tetradecanoic acid (+atp) has shown that two polypeptides found in the fatty acid reductase complex, the fatty acid activating enzyme (50k) and the 34k polypeptide, were specifically labeled during induction of the luminescent system. an alternate method for in vitro acylation of polypeptides in the luminescent system was developed using tetradecanoyl-coa. both the 34k polypeptide and, to a lesser extent, ...19846693412
bioluminescence procedures for the measurement of nad(p) dependent enzyme catalytic activities in submicrogram quantities of rabbit and human nephron structures.reduced flavin mononucleotide dependent luciferase (ec 1.14.14.3) from photobacterium fischeri has been used to measure nad(p) dependent enzymes in submicrogram quantities of tissue homogenates and isolated structures of rabbit and human kidney. the procedure for measuring nad(p)h was optimized, with internal standardization, to give a linear constant signal between 1 and 100 pmol. this method was applied to the measurement of glucose-6-phosphate dehydrogenase (ec 1.1.1.49) and 3-hydroxybutyrate ...19846716053
[isolation and purification of bacterial luciferase from photobacterium fischeri for analytical purposes].a procedure resulting in a highly purified preparation of bacterial luciferase with a high specific activity towards fmnh2 and nadh was developed. using sds-electrophoresis in polyacrylamide gel, it was shown that the enzyme has a subunit composition and that its monomers do not contain the luciferase activity. the use of the obtained preparation for determining the content of nadh and fmn by the bioluminescent method allowed to increase its sensitivity up to 10(-18) and 10(-17) moles, respectiv ...19807248358
vibrio harveyi aldehyde dehydrogenase. partial reversal of aldehyde oxidation and its possible role in the reduction of fatty acids for the bioluminescence reaction.vibrio harveyi aldehyde dehydrogenase, which catalyzes the oxidation of long chain aliphatic aldehydes to acids, has been discovered to have both acyl-coa reductase and thioesterase activities. tetradecanoyl-coa was reduced to tetradecanal in the presence of nad(p)h, as monitored by the stimulation of luciferase activity by the aldehyde product (acyl-coa reductase). in the absence of nadph, [3h]tetradecanoyl-coa was hydrolyzed to the hexane-soluble fatty acid (thioesterase). inhibition data with ...19846725283
[inhibition of bacterial luminescence by cytochrome p-450 substrates].the mechanisms of luminescence quenching by various drugs, e.g. dimethylaniline, ethylmorphine, hexobarbital and aminopyrine, which are effective inhibitors of luminescence both in intact cells and in bacterial luciferase, were studied. it was shown that the inhibition of luminescence occurs due to competition of the bacterial luminescence system substrate--aliphatic aldehyde in cytochrome p-450. the functional similarity of the bacterial luminescence system to the microsomal hydroxylation syste ...19817248381
bioluminescent toxicity assay of synfuel by-product waters. 19846733307
dna-damaging agents and dna-synthesis inhibitors induce luminescence in dark variants of luminous bacteria.the dna-damaging agents mitomycin c and uv irradiation, as well as the dna-synthesis inhibitors nalidixic acid, novobiocin and coumermycin, induce the de novo synthesis of luciferase and in vivo luminescence in dark variant cells of the luminous bacteria photobacterium leiognathi. mitomycin c and nalidixic acid also cause the induction of luminescence in wild-type cells in the absence of its natural inducer. in spite of the high level of in vivo luminescence of the treated dark-variant cells, no ...19817290100
flavine specificity of enzyme-substrate intermediates in the bacterial bioluminescent reaction. structural requirements of the flavine side chain. 19734699979
a membrane-covered photobacterium probe for oxygen measurements in the nanomolar range. 19817304978
[pyruvic acid formation by the luminescent bacterium photobacterium mandapamensis]. 19817321908
isolation of d-erythro-neopterin 2':3'-cyclic phosphate from photobacterium phosphoreum. 19734699996
adjuvant effect of photobacterium phosphoreum pj-1 on humoral immune response of ddy mice to sheep erythrocytes. 19817349282
conversion of aldehyde to acid in the bacterial bioluminescent reaction. 19734796920
[electron transport systems of photobacterium fischeri].the composition of cytochromes was studied in photobacterium fischeri 6 at different growth phases and under various conditions of cultivation. the electron transport chains of the bacterium are characterized by the presence of cytochromes b and c types. the terminal oxidases are cytochromes o, a2+a1 and p-450. the hemoprotein p-450 functions as a mixed function oxidase. the qualitative composition of cytochromes does not depend on the growth phase of the bacterium but does on the conditions of ...19807402117
[optimization of the nutrient medium composition for bacteria of the genus photobacterium]. 19734799686
taxonomy of marine bacteria: the genus beneckea.one-hundred-and-forty-five isolates of marine origin were submitted to an extensive physiological, nutritional, and morphological characterization. all strains were gram-negative, facultatively anaerobic, straight or curved rods which were motile by means of flagella. glucose was fermented with the production of acid but no gas. sodium but no organic growth factors were required. none of the strains were able to denitrify or fix molecular nitrogen. the results of nutritional and physiological te ...19714935323
the effect of temperature on the potency of halothane. 19725016897
nutritional requirements of some marine luminous bacteria. 19715087892
oxygen affinities of the hydrogenosome-containing protozoa tritrichomonas foetus and dasytricha ruminantium, and two aerobic protozoa, determined by bacterial bioluminescence.oxygen-dependent bioluminescence of photobacterium (vibrio) fischeri was used to measure oxygen affinities of four protozoa. the aerobic organisms acanthamoeba castellanii and tetrahymena pyriformis showed apparent km values for o2 of 0.42 and 2.43 microm respectively. the aerotolerant anaerobe tritrichomonas foetus, and the more strictly anaerobic rumen ciliate dasytricha ruminantium, both of which have hydrogenosomes, respired with apparent km values of 1.08 and 1.70 microm-o2. we conclude tha ...19826809887
nitrogen fixation as evidence for the reducing nature of the early biosphere.probably the first nitrogen fixers were anaerobic, non-photosynthetic, bacteria, i.e. fermenters. during the evolution of n2 fixation they still needed nitrogen on the oxidation level of ammonia. because of the complexities in structure and function of nitrogenase this evolution must have required a long time. the photosynthetic and later the respiring bacteria inherited the capacity for n2 fixation from the fermenters, but the process did not change a great deal when it was taken over. because ...19836871385
[pathway of synthesis of the aldehyde factor - a basic substrate of luciferase].the stimulation of luminescence and cell aldehyde factor during the growth of one-aldehyde-dependent mutant in the condition medium by other aldehyde-dependent mutants was studied. it was shown that the aldehyde factor is synthesized in five successive steps, thus suggesting the participation of five enzymes in aldehyde factor synthesis. the metabolite accumulation in the condition medium increases both the level of the aldehyde factor and that of luciferase. it is assumed that some precursors o ...19836882834
acridine dyes and other dna-intercalating agents induce the luminescence system of luminous bacteria and their dark variants.acridine dyes and other dna-intercalating agents such as ethidium bromide, theophylline, and caffeine induce luminescence in dark variants (k variants) different luminous species of bacteria, as well as in their wild-type luminous cells, prior to induction. the increase in luminescence appears 10-20 min after addition of these agents and is inhibited by chloramphenicol or rifampicin. addition of these agents affects the synthesis of both luciferase and aldehyde-synthesizing enzymes. it is hypoth ...19816943543
reaction kinetics in living systems.in this report we treat reaction rates, equilibrium theory, and irreversible thermodynamics as different aspects of a single discipline. in biological reactions the rate is ultimately controlled by enzymes and other proteins of complex structure and high molecular weight. the needed formalism can be placed in one-to-one correspondence with appropriate electrical and mechanical networks. an enzyme molecule has zwitter ions anchored in the polypeptide chain, which enable it to distort the substrat ...19816946491
bioluminescence from single bacterial cells exhibits no oscillation.since the usual measurements of light emission from marine bacteria involve many (10(6)-10(10)) cells, the question has often been raised as to whether or not the individual cell's luminescence is truly continuous. to investigate this question, we assembled a sensitive photo-counting system with computerized data acquisition. several luminous species were studied: beneckea harveyi, photobacterium belozerskii, p. fischeri, and p. leiognathi. isolated single cells gave count rates ranging from 2 t ...19806973368
immunological properties of lipopolysaccharide from a marine bacterium, photobacterium phosphoreum pj-1. 19826982391
isolation of bacterial luciferases by affinity chromatography on 2,2-diphenylpropylamine-sepharose: phosphate-mediated binding to an immobilized substrate analogue.a covalently immobilized form of an inhibitor of bacterial luciferase, 2,2-diphenylpropylamine (d phi pa), was an effective affinity resin for purifying this enzyme from several distinct bacterial species. the inhibitor is competitive with the luciferase aldehyde substrate but enhances binding of the flavin substrate fmnh2 (reduced riboflavin 5'-phosphate); comparable binding interactions occur with luciferase, the immobilized inhibitor d phi pa-sepharose, and the substrates [holzman, t. f., & b ...19826983889
identification of vibrio hollisae sp. nov. from patients with diarrhea.the name vibrio hollisae (synonym = special bacteriology group ef-13) is proposed for a new group of 16 strains that occurred in stool cultures of patients with diarrhea. v. hollisae is a small gram-negative rod, which is motile with a single polar flagellum. no lateral or peritrichous flagella were observed, even when it was grown on a solid medium. sodium chloride is required for growth, so v. hollisae is a halophilic vibrio. strains were positive (36 degrees c, 24 or 48 h) for oxidase (kovacs ...19827076812
Displaying items 301 - 400 of 1266