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a pulse-radiolysis study of the catalytic mechanism of the iron-containing superoxide dismutase from photobacterium leiognathi.the mechanism of the enzymic reaction of an iron-containing superoxide dismutase purified from the marine bacterium photobacterium leiognathi was studied by using pulse radiolysis. measurements of activity were done with two different preparations of enzyme containing either 1.6 or 1.15 g-atom of iron/mol. in both cases, identical values of the second-order rate constant for reaction between superoxide dismutase and the superoxide ion in the ph range 6.2-9.0 (k=5.5 x 10(8) m-1-s-1 at ph 8.0) wer ...197715540
interaction of the mannosephilic lectins of pseudomonas aeruginosa with luminous species of marine enterobacteria.the marine bacteria beneckea harveyi and photobacterium leiognathi were shown to bear mannose-containing binding sites for the mannosephilic lectins of pseudomonas aeruginosa and concanavalin a (con a). the interaction between the lectins and the marine bacteria was demonstrated by the bacteriagglutination test, by adsorption of the lectins onto the bacteria and by mannose-specific peroxidase-binding to the lectin-coated bacteria. treatment of the bacteria with formaldehyde, phenol, ethanol or b ...1979120927
bacterial bioluminescence. 1977199107
kinetic studies on the mechanism of bacterial nad(p)h:flavin oxidoreductase. 1979222213
biosynthesis of aliphatic aldehydes for the bacterial bioluminescent reaction: stimulation by atp and nadph. 1979223549
luminescence in clinical analysis. 1979231928
[biochemical bases of bioluminescence]. 1975235818
blue--green bacteria: status and photoqutotrophic metabolism. 1975236953
ferredoxins, blue-green bacteria and evolution. 1975236955
reaction rate theory in bioluminescence and other life phenomena. 1977326144
high molecular weight blue fluorescence protein from the bioluminescent bacterium photobacterium fischeri. 1979435324
[genetic studies of photobacterium mandapamensis. ii. the classification of mutants with an altered luminescence intensity according to their sensitivity to exogenous aldehyde].the collection of 157 dark and dim photobacterium mandapamensis strains was divided into four groups using the addition of 0.2 ml of 0.15% myristis aldehyde to cell suspension. the luminescence did not change in the presence of the aldehyde in 76 mutants, it decreased in 30 mutants, it was 2--8-fold increased in 35 mutants, and it was increased more than 10-fold in 16 mutant strains. 19 strains of those having luminescence in the presence of the aldehyde have mutations in genes controlling the b ...1979488711
[ultrastructural organization of marine luminescent bacteria and their mutants].the aim of this work was to study the submicroscopic organization of luminescent bacteria belonging to the genera photobacterium and lucibacterium as well as that of their "dark" mutants incapable of luminescence. the ultrastructural organization of all studied bacteria is typical of gram-negative species. the luminescent bacteria are characterized by the presence, in their cytoplasm, of osmophilic formations 22--110 nm in size. the cells of "dark" mutants accumulate volutin and contain complex ...1979530133
an experimental model of the krogh tissue cylinder: two dimensional quantitation of the oxygen gradient. 1977613755
separation of a blue fluorescence protein from bacterial luciferase. 1978623648
sensitive oxygen assay method by luminous bacteria. 1978732582
a calorimetric investigation of the growth of the luminescent bacteria beneckea harveyi and photobacterium leiognathi.direct calorimetric determinations of the rate of heat production along with simultaneous determinations of the rate of photon emission and the number of viable cells have provided insight into the growth of beneckea harveyi and photobacterium leiognathi. these experiments were performed with a tronac isothermal microcalorimeter modified with a fiber optic light guide to allow in situ detection of light. escherichia coli and a dark variant of p. leiognathi were also examined to provide points of ...1977401656
applications of bio- and chemiluminescence in the clinical laboratory. 1979380839
5s rna secondary structure. 1975808733
characterization of some fish and shrimp spoiling bacteria.the classification of some important groups of bacteria involved in fish and shrimp spoilage was studied. trimethylamine is produced by pseudomonas putrefaciens, a "non-defined" group resembling ps. putrefaciens, photobacterium spp. and some moraxella-like bacteria. hypoxanthine is produced by the same groups of bacteria except the last named and also by the "typical shrimp spoilers" (presumptive alteromonas). strong off-odours are produced on fresh fish by ps. putrefaciens, dextrose-oxidative p ...1977341802
biology of the marine enterobacteria: genera beneckea and photobacterium. 1977334043
covalent structure of subunits of bacterial luciferase: nh2-terminal sequence demonstrates subunit homology.the heterodimeric subunit structure of bacterial luciferase was demonstrated more than 10 years ago. the enzymes from both beneckea harveyi and photobacterium fischeri have since been studied in detail; they each consist of two nonidentical subunits, designated alpha and beta. both are required for bioluminescence activity, with the active center apparently confined to the alpha subunit. amino acid sequence analysis of the nh2 termini of the alpha and beta subunits of the b. harveyi and p. fisch ...1979315557
pyruvate production and excretion by the luminous marine bacteria.during aerobic growth on glucose, several species of luminous marine bacteria exhibited an imcomplete oxidative catabolism of substrate. pyruvate, one of the products of glucose metabolism, was excreted into the medium during exponential growth and accounted for up to 50% of the substrate carbon metabolized. when glucose was depleted from the medium, the excreted pyruvate was promptly utilized, demonstrating that the cells are capable of pyruvate catabolism. pyruvate excretion is not a general p ...1977303077
bioluminescence: from chemical bonds to photons.the biological transformation of chemical to photic energy involves an enzyme-mediated chemiluminescent reaction, in which one of the products exists in an electronically excited state, emitting a photon as it returns to the ground state. the colour of bioluminescence differs in different organisms, ranging from the deep blue (460 nm) of certain crustacea, through the bluish green (490 nm) of some bacteria, the green (530 nm) of mushrooms to the red (about 600 nm) of the railroad worm. in one ca ...1975238805
the isolation of a bacterial glycoprotein with luciferase activity. 1977900939
chemical modification studies on a ferri-superoxide dismutase from marine bacteria. 1977913572
superoxide dismutases: defence against endogenous superoxide radical.attempts to measure the rate of o2- production, in whole cells or in intact subcellular organelles, are frustrated by the endogenous superoxide dismutase (sod). streptococcus faecalis contains a single manganese-sod which was isolated and used as an antigen in the rabbit. a precipitating and inhibiting antibody was obtained and used to suppress the sod in crude lysates of s. faecalis. it allowed the demonstration that 17% of the total oxygen uptake by such lysates, in the presence of nadh, was a ...1978225147
electron paramagnetic resonance spectra of myeloperoxidase in polymorphonuclear leukocytes. 1978202508
enzymes of d-fructose catabolism in species of beneckea and photobacterium.cell-free extracts of strains representative of the genera beneckea and photobacterium catalyzed a p-enolpyruvate dependent phosphorylation of d-fructose. the resulting product, fructose-1-p, was converted to fructose-1,6-p2 by 1-p-fructokinase. both activities were inducible, being present in d-fructose-grown cells and reduced or absent in d-gluconate-(or succinate-) grown cells.1975125566
[interaction of dexamethasone-receptor complexes with rat liver nuclei and dnas].the role of dnas in the nuclear binding of dexamethasone-receptor complexes (drc) was studied. the cytosolic receptors from rat liver have a sedimentation coefficient of about 7s, the stock's radius--of about 50 a and possess a high affinity to dexamethasone (kas = 2,6 x 10(8) m-1). their capacity is 3 x 10(-13) and 5.5--7.0 x 10(-12) mole of dexamethasone per mg cytosolic protein and mg dna, respectively. drc has the ability to bind to the nuclei of rat liver. drc binding to nuclei is increased ...19761022278
proceedings: evidence for specific nadh- and nadph-fmn reductases in bacterial bioluminescence. 197554079
the continuous culture of luminous bacteria: a luminostat. 19751107290
flavin mononucleotide reductase of luminous bacteria.nad(p)h: fmn oxidoreductase (flavin reductase) couples in vitro to bacterial luciferase. this reductase, which is also postulated to supply reduced flavin mononucleotide in vivo as a substrate for the bioluminescent reaction, has been partially purified and characterized from two species of luminous bacterial. from photobacterium fischeri the enzyme has a m. w. determined by sephadex gel filtration, of 43,000 and may have a subunit structure. the turnover number at 20 degrees c, based on a purit ...197547604
myristic acid stimulation of bacterial bioluminescence in "aldehyde" mutants.the involvement of long chain aldehyde in bacterial luminescence was known both from its being required for light emission in the in vitro reaction with pure luciferase and from its ability to stimulate luminescence in vivo in a certain class of dark "aldehyde" mutants. we have found that the luminescence of some (but not all) of such aldehyde mutants is also stimulated by long chain aliphatic fatty acids, with a marked specificity for myristic (tetradecanoic) acid. this stimulation has been dem ...197824214
luminescence and respiratory activities of photobacterium phosphoreum. competition for cellular reducing power.changes in the in vivo luminescence, respiratory activities, contents of cytochromes, extractable luciferase and nad(p)h-fmn reductase during growth of the wild (bright) strain of photobacterium phosphoreum and its dim mutant were determined. the intensity of the in vivo luminescence per cell increased 10 times in the wild strain and 750 times in the dim strain during logarithmic growth, while the contents of luciferase and nad(p)h-fmn reductase remained almost constant. it is suggested that a c ...19755397
properties and kinetics of salt activation of a membrane-bound nadh dehydrogenase from a marine bacterium photobacterium phosphoreum.a membrane-bound nadh dehydrogenase, solubilized and partially purified from a marine bacterium photobacterium phosphoreum, contains fad as the prosthetic group, and is specific for nadh. ferricyanide, various other redox dyes and cytochrome c can act as electron acceptors. the enzymatic activity when assayed with electron acceptors other than cytochrome c, is activated by monovalent cations (na+ and k+) and deactivated by high concentrations of monovalent anions (scn-, no3-, and cl-) but not by ...1978721793
control of luciferase synthesis in a newly isolated strain of photobacterium leiognathi.in previous studies with luminous bacteria of all different species it has been reported that the synthesis of luciferase is autoinducible: during growth at low cell densities synthesis is effectively repressed while after induction, at higher cell densities, the rate of synthesis of enzyme is up to five times the growth rate. in this paper we report on newly isolated strains of photobacterium leiognathi which show continued luciferase synthesis irrespective of the cell density. the specific syn ...1977603341
luminescence and respiratory activities of photobacterium phosphoreum. ii. control by monovalent cations.luminescent activity of spheroplasts of the cells of photobacterium phosphoreum was stimulated by rb+ and k+ and inhibited by na+ in the medium. opposite effects of these ions were observed on the rate of o2 consumption of the spheroplasts through the cytochrome and luciferase electron transfer systems. in vitro activities of nadh-fmn reductase and luciferase were only slightly stimulated by rb+, k+, and na+, while na+ exhibited significant activation of the nadh-oxidizing activity of the cells. ...1977599151
mechanism of inhibition of bacterial luciferase by anaesthetics. 19751219299
evidence for a fatty acid reductase catalyzing the synthesis of aldehydes for the bacterial bioluminescent reaction. resolution from luciferase and dependence on fatty acids.the enzyme responsible for the stimulation by atp and nadph of light emission catalyzed by bacterial luciferase has been partially purified from extracts of the luminescent bacterium, photobacterium phosphoreum. the stimulatory activity was found to be stabilized by high concentrations of mercaptoethanol, permitting it to be separated from luciferase into an active and stable form and enabling further characterization of its functional properties. the activity of the enzyme was shown to be depen ...1979572825
the terminal oxidase of photobacterium phosphoreum. a novel cytochrome.the terminal oxidase of photobacterium phosphoreum has been purified to the electrophoretically homogeneous state and some of its properties have been studied. the enzyme catalyses oxidation of ascorbate in the presence of phenazine methosulphate or n,n,n',n'-tetramethyl-p-phenylenediamine. the reaction is inhibited by cyanide. nitrite at comparatively high concentrations inhibits the enzyme, but the enzyme does not catalyse nitrite reduction with ascorbate plus the electron mediator as the elec ...1979465487
bacterial bioluminescence: equilibrium association measurements, quantum yields, reaction kinetics, and overall reaction scheme.the characteristics of the bioluminescence reactions with bacterial luciferase from two different cell types, photobacterium fischeri and beneckea harveyi, are reported. the reduced flavine mononucleotide (fmnh2)-luciferase association constant, directly measured by equilibrium dialysis and gel filtration is the same for both luciferases, 3 times 10(-4) mminus1 at romm temperature, and is significantly different from the kinetic reciprocal michaelis-menten constant. the luciferase bioluminescenc ...1975807236
studies on luciferase from photobacterium phosphoreum. xi. interaction of 8-substituted fmnh2 with luciferase.the interaction of bacterial luciferase from photobacterium phosphoreum with reduced flavin was investigated using various 8-substituted fmnh2 analogs. flavins tested were fmnh2 and fmnh2 substituted at the 8 position with ho-, ch3o-, c2h5o-, cl-, br-, i-, h2n-, (ch3)hn-, and (ch3)2n. 8-ch30-, c2h5o-, cl-, and br-fmnh2 showed luminescent activity in the luciferase reaction with emission peaks at various wavelengths. 8-ho- and i-fmnh2 were competitive inhibitors toward fmnh2 in the luminescent re ...1978738995
[isolation of bacterial luminescence reaction inhibitor from photobacterium sp. cells].the factor having a strong inhibitory effect on bacterial luminescence was isolated from the luminous bacteria species photobacterium sp. the inhibitor purified by gel filtration on the biogel and by deae chromatography was homogenous (single bound during electrophoresis in polyacrylamide gel), it reacted with coomassie brilliant blue and gave a positive lowry reaction on protein. molecular weight was about 30,000 as determined by sds-polyacrylamide gel electrophoresis. the absorption spectrum w ...1978737225
[luminescence and growth of photobacterium mandapamensis in periodic culture].the luminescent photobacterium mandapamensis, strain 54 (the collection of the institute of physics, siberian branch of the ussr academy of sciences), was isolated from the water of the pacific ocean in the equatorial zone and studied in the course of periodic cultivation. the dynamics of changes in the main parameters of the culture, such as luminescence, growth, respiration, heat emission etc., was investigated. the data obtained were used to establish a correlation between changes in these pa ...1978713875
studies on luciferase from photobacterium phosphoreum. x. heat of formation of the intermediate in the bioluminescent reaction studied by stopped-flow calorimetry.the heat production in the reaction of luciferase-fmnh2 complex with o2 in the absence of aldehyde was measured by stopped-flow calorimetry. deltah of the reaction, luciferase-fmnh2+ o2 leads to intermediate x1, is -1.3 x 10(2) kj.mol-1 and the calculated deltas for the reaction is -180 j.mol-1.k-1 at 20 degrees c. the heat production in the bioluminescent reaction was also measured in the presence of a saturating concentration of aldehyde, and it was estimated that 43 and 79% of the c10 and c13 ...1978659382
[determination of trace amounts of 2,4-dinitrofluorobenzene by a bioluminescence method]. 1979508879
autoinduction of bacterial luciferase. occurrence, mechanism and significance.the synthesis of the luminous system of the marine luminous bacterium photobacterium fischeri is subject to a complex, self-regulated control system called autoinduction. the bacteria produce an autoinducer which accumulates in the medium at a constant rate (as a function of cell growth). when autoinducer reaches a critical concentration it stimulates, at the level of transcription, the synthesis of the luminous system. autoinduction is thus viewed as an environmental sensing mechanism, which cu ...1977843170
low oxygen is optimal for luciferase synthesis in some bacteria. ecological implications.the synthesis of the bioluminescent systems in many strains of two species of the genus photobacterium which were isolated as symbionts is greater at low oxygen concentrations, where aerobic growth is blocked. in strains of two other species, one photobacterium of symbiotic orgin, and one (genus beneckea) whose lent response is observed. at low oxygen concentrations, where there is an inhibition of growth, there is also a similar decrease in the synthesis of the luminescent system. these species ...1977843171
a luminous bacterium that emits yellow light.a strain of photobacterium fischeri that emits yellow light has been isolated from seawater. the bimodal spectrum, which is unique among the luminous bacteria, consists of a major band with a maximum at 545 nanometers and a minor band with a maximum at 500 nanometers. the former represents a heretofore unreported range of emission for luminous bacteria, while the latter coincides with the emission spectrum of typical blue-greeen-emitting strains of p. fischeri. the relative contributions of thes ...1977850787
purification and properties of the nadh and nadph specific fmn oxidoreductases from beneckea harveyi.the nadh and nadph specific fmn oxidoreductases from beneckea harveyi have been purified to homogeneity as judged by single bands on sodium dodecyl sulfate gel electrophoresis. the overall purification for the nadh specific enzyme is 3000-fold and 4000-fold for the nadph specific enzyme from a crude extract. the final step in the purification procedure is chromatography on a 5'-amp-sepharose 4b affinity column which results in approximately a 50-fold purification to a final specific activity of ...1977880288
studies on luciferase from photobacterium phosphoreum. ix. further studies on the spectroscopic characteristics of the enzyme-fmn intermediates.the absorption and fluorometric changes of the reaction mixture of luciferase-fmnh2 complex with o2 were re-examined. rapid formation (k2(app) = 2.0 s-1 at [o2] = 120 micrometer) of an intermediate with a single absorption maximum at 380 nm within the range of 350-550 nm, and a weak fluorescence at 520 nm (less than or equal to 10% of that of fmn when excited at 380 nm) was observed. the absorption and fluorescence spectra and decay rate of the intermediate were estimated by simulation using an ...1977881410
studies on luciferase from photobacterium phosphoreum. viii. fmn-h2o2 initiated bioluminescence and the thermodynamics of the elementary steps of the luciferase reaction.bacterial luciferase from photobacterium phosohoreum was found to produce bioluminescence on reaction with fmn and h2o2 in the presence of aldehyde. this luminescence is presumably produced by the same x1 intermediate as that found in fmnh2-o2 initiated luminescence. from the ratio of the light intensities of the fmn-h2o2 initiated reactions, we calculated the association constant of the reaction, luciferase+fmn+h2o2in equilibriumx1, and estimated its temperature dependence. from these results w ...1976950335
symbiotic association of photobacterium fischeri with the marine luminous fish monocentris japonica; a model of symbiosis based on bacterial studies.isolation of bacteria from the luminous organ of the fish monocentris japonica has revealed that the organ contains a pure culture of luminous bacteria. for the four fish examined, all contained photobacterium fischeri as their luminous bacterial symbiont. this is the first time that p. fischeri has been identified in a symbiotic association. a representative isolate (mjl) of the light organ population was selected for in vivo studies of its luminous system. several physiological features sugge ...19761016667
bacterial luciferase requires one reduced flavin for light emission.recent reports revive a hypothesis that the bacterial bioluminescence reaction involves two reduced flavin mononucleotide molecules per enzyme turnover. a two-flavin mechanism requires that the two flavins bind simultaneously or sequentially to the same or different sites on luciferase during a catalytic cycle. measurements using equilibrium techniques show that the luciferase dimer has only a single reduced flavin binding site. quantum yield results demonstrate that bioluminescence requires onl ...19751059124
primary chemical and physical characterization of acute toxic components in wastewaters.a chemical and physical primary characterization work sheet was developed based on the microtox test, a bacterial bioluminescence system used as a rapid estimate of acute aquatic toxic effects. measurements of the variation in light reduction upon different pretreatments provided information about the chemical and physical properties of the main toxic component(s) in test wastewater samples. this primary characterization of a wastewater sample was performed within 1 day. tests of pure toxic chem ...19921280588
study of genetic relationships among marine species of the genera beneckea and photobacterium by means of in vitro dna/dna hybridization.strains representative of species of the marine genera beneckea and photobacterium were used as reference standards in in vitro dna/dna competition experiments. within a given species, strains were found to be related by over 80% competition. (competition was defined as the amount of radioactive dna displaced by heterologous dna relative to the amount displaced by homologous dna.) on the basis of interspecies competition values (expressed as averages), the following groupings could be made: 1. " ...19761015934
[cellular protrusions of luminescent bacteria and relation of their appearance to cultivation conditions].the luminescent cells of photobacterium were studied by electron microscopy in order to establish what caused their sticking together and growth on the glass walls of the vessel during continuous cultivation. electron microscopy revealed protrusions, of various shape and size, on the cells. the phenomenology of various protrusions, including fimbria, is described, and the effect of cultivation conditions (continuous culture, periodic culture) and growth phases on their emergence was elucidated. ...19751160626
identification of nadh-specific and nadph-specific fmn reductases in beneckea harveyi.distinct fmn reductases specific for nadh and nadph were identified in extracts of beneckea harveyi. these enzymes differ in their physical (molecular weight, thermostability) as well as in their chemical properties (binding constants for nadh and nadph). the nadh-specific enzyme is more efficient than the nadph-specific one with respect to the bioluminescent reaction.19751175652
the nucleotide sequence of the 5s ribosomal rna from a photobacterium.comparative sequencing studies provide powerful insights into molecular function and evolution. the sequence for 5s ribosomal rna from photobacter strain 8265 is eighteen base replacements removed from that of escherichia coli. of these, the vast majority involve a g or c becoming an a or u. these variations also define unequivocally a hexanucleotide base paired region, which appears to be a universal feature of the 5s rna molecule. the base composition of this helix seems to be under rather str ...19751177325
[impulse characteristics of bacterial bioluminescence]. 19751183308
[deoxyribonucleic acid hybridizations among some vibrio-like marine bacteria (author's transl)].the genotypic relationships established by dna/dna hybridization in vitro confirm the results obtained by earlier phenotypic analyses of certain vibrio-like marine bacteria. these strains are closely related to the species photobacterium fischeri, and do not belong to the genus vibrio. the group of marine, vibrio-like bacteria that require na+ for growth is genotypically very heterogeneous.19751190947
the lux genes in photobacterium leiognathi are closely linked with genes corresponding in sequence to riboflavin synthesis genes.three open reading frames (orfs) have been found in the region downstream of the luxg gene in the photobacterium leiognathi lux operon. these genes (orf i, ii, and iii) are not only closely linked to the lux operon and transcribed in the same direction but also show the same organization and code for proteins homologous in sequence to the gene products of ribb, riba, and ribh of bacillus subtilis, respectively. the photobacterium leiognathi gene (orf ii) corresponding to riba was expressed in es ...19921339274
evidence for the existence of a restriction-modification system common to several species of the family vibrionaceae.a broad-host-range vibriophage kvp40 originally isolated on vibrio parahaemolyticus 1010 was restricted and modified by strains of at least five vibrio and one photobacterium species. 1010 was a non-restricting host. an anti-restriction mutant kvp40 aar1 was isolated after propagating the phage on a restricting host, v. anguillarum vib36. kvp40 aar1 grown on either 1010 or vib36, as well as the parental phage grown on vib36, showed much higher efficiencies of plating on all the restricting hosts ...19921521769
use of a rapid bioluminescence assay for detecting cyanobacterial microcystin toxicity.the recent rise in the awareness of the occurrence of toxic cyanobacterial blooms in aquatic environments, with associated human health problems and animal deaths, has increased the need for rapid, reliable and sensitive methods of determining cyanobacterial toxicity. a luminescent bacterial toxicity test was assessed as a complement to the established mouse bioassay. seventeen samples including pure cyanobacterial microcystin-lr hepatotoxin, laboratory isolates and natural blooms of cyanobacter ...19901367480
[possibilities for using biotests for the assessment of the hazard potential of ground water with hazardous sediments]. 19921339172
[microbial biotests with sediments]. 19921307811
[experiences with biologically active tests in the study of soil pollutants]. 19921307812
development of monoclonal antibodies that identify vibrio species commonly isolated from infections of humans, fish, and shellfish.monoclonal antibodies (mabs) against vibrio species that infect humans, fish, and shellfish were developed for application in rapid identifications. the pathogens included vibrio alginolyticus, v. anguillarum, v. carchariae, v. cholerae, v. damsela, v. furnissii, v. harveyi, v. ordalii, v. parahaemolyticus, and v. vulnificus. three types of mabs were selected. the first important group included mabs that reacted with only a single species. a second group comprised a number of mabs that reacted w ...19921482190
investigations of phagocytosis concerning the immunological defence mechanism of mytilus edulis using a sublethal luminescent bacterial assay (photobacterium phosphoreum).1. a simple method for the determination of phagocytosis activity using mussel hemocytes by measuring the bioluminescence is presented. 2. the immunological defence activity based on phagocytosis is measured and quantified by a luminescent bacterial assay with photobacterium phosphoreum. 3. the measuring system allows us to establish the stress of the immunological defence mechanism of organisms exposed to chemicals and polluted rivers or sewage. results with reference substances and the phagocy ...19911677842
[biotest studies for evaluating hazardous waste water with reference to section 7a whg]. 19921307816
[biological test procedures in compliance with abwag and whg]. 19921307817
fluorescence study of the ligand stereospecificity for binding to lumazine protein.6,7-dimethyllumazine derivatives, substituted at the 8-position with aldityls or monohydroxyalkyl groups, have been examined for their binding ability to lumazine apo-protein from two strains of photobacterium phosphoreum using fluorescence dynamics techniques. on the protein the lumazine has a nearly monoexponential decay of fluorescence with lifetime 13.8 ns (20 degrees c). in free solution the lifetime is 9.6 ns. the concentration of free and bound lumazine in an equilibrium mixture can be re ...19921483455
toxicity assessment of atrazine, alachlor, and carbofuran and their respective environmental metabolites using microtox.using the microtox method of toxicity assessment designed by microbics corporation, the relative toxicities of alachlor, atrazine, and carbofuran, three pesticides commonly used in agricultural production, were determined. generally, carbofuran was found to be most acutely toxic, followed closely by atrazine. alachlor was least toxic of the three pesticides tested. selected environmental metabolites of these three agri-chemicals were also tested using the same method. hydroxyalachlor, deethylatr ...19921522608
determination of petroleum hydrocarbon toxicity with microtox. 19911786452
[eco-toxicologic studies of the effect of boat engine emissions]. 19921307785
[detection of hazardous substances in waste water with reference to biodegradability and toxicity for evaluating the status of the technique exemplified by gas station and automobile repair shop waste water]. 19921307818
evaluation of the genotoxic activity and acute toxicity of euphorbia splendens latex, a molluscicide for the control of schistosomiasis.1. the latex of euphorbia splendens var. hislopii has a molluscicidal action at low concentration (ld90 less than 1.5 ppm or 1.5 micrograms/ml) against the vector snails of schistosomiasis. 2. in the present study, the latex in natura or after lyophilization was submitted to the ames test and the chromotest to evaluate genotoxicity, to the microtox system to determine acute toxicity, and to the chinese hamster ovary cell assay (cho) to measure cytotoxicity. 3. the latex had no mutagenic activity ...19911823273
[continuous biological test procedures for monitoring waste water and event controlled sampling techniques]. 19921307793
[a biotest program for risk evaluation of sediments]. 19921307810
[the luminescent bacteria test for clean water legislation].luminescent bacteria (photobacterium phosphoreum) may be used, on the one hand, for classical toxicity tests based on the inhibition of respiration or growth and, on the other hand, their luminescence itself may become the test criterion. such a luminescence inhibition test is commonly called a luminescent bacteria test. it may be conducted with fresh bacteria or with conserved ones. the luminescence parameter is, just like the parameter oxygen consumption, a summative parameter for undisturbed ...19921307824
[the physiologic background of the luminescent bacteria test]. 19921307825
[the luminescent bacteria test with personally cultured and freeze dried bacteria]. 19921307826
[environmental monitoring with the luminescent bacteria test: the problem of false negative findings--short report]. 19921307827
activity and stability of the luciferase--flavin intermediate.a luciferase intermediate in the bacterial bioluminescence system, which is formed by reaction of enzyme with reduced flavin mononucleotide (fmnh2) and oxygen, is shown to emit light with added aldehyde under anaerobic conditions. the reaction with oxygen is thus effectively irreversible under the conditions used. the flavin chromophore has an absorption maximum at about 370 nm and the potential activity (bioluminescence yield) in the further reaction of the isolated intermediate with aldehyde i ...1978306832
a novel method for detection and counting of single bacteria in a wide field using an ultra-high-sensitivity tv camera without a microscope.we describe a novel method for enumeration of bacteria, based on the principle that small, light emitting particles on a flat surface can be easily and rapidly detected and counted using an ultra-high-sensitivity tv camera. to test this method, we obtained tv images of individual cells of a luminous bacterium on a membrane filter without the use of a microscope. the positions of the luminous points in the tv images were almost the same as the positions of the bacterial colonies after growth. our ...19911916227
[examination of the water supply with the alkali and alkali-earth supplement optimized din luminescent bacteria test, exemplified by the saar river].the light intensity emitted by luminescent bacteria is influenced by both the osmolarity and the ion composition of the test medium. the addition of potassium and calcium ions to a sodium chloride solution causes a considerable increase in the light intensity of bacteria. if these elements occur in the proper concentrations in the test material, the luminescence in the test sample will be higher than in the control sample containing only sodium chloride. this physiological dependence did not fin ...19921307828
[luminescence, growth and respiration as end points of the toxic effects on photobacterium phosphoreum--short report]. 19921307830
mutatox: a genotoxicity assay using luminescent bacteria. 19921307839
bioluminescent method in studying the complex effect of sewage components.the inhibition of bacterial luminescence has been used in testing industrial enterprises sewage. the toxicity of the sewage is less than the total toxicity of separate components due to neutralization of quinone products of polyphenol oxidation in the reactions with the other phenol components of sewage. toxicity increase is due to their influence on the cell membrane. studies of cell ultrastructure confirm this fact. the studied mechanism of the complex effect allowed a more accurate forecast o ...19921536600
[osmiophilic formations in cells of luminous bacteria]. 19751140069
integrated assessment of contaminated sediments in the lower fox river and green bay, wisconsin.samples of sediment and biota were collected from sites in the lower fox river and southern green bay to determine existing or potential impacts of sediment-associated contaminants on different ecosystem components of this great lakes area of concern. evaluation of benthos revealed a relatively depauperate community, particularly at the lower fox river sites. sediment pore water and bulk sediments from several lower fox river sites were toxic to a number of test species including pimephales prom ...19921375148
a broad-host-range vibriophage, kvp40, isolated from sea water.a broad-host-range vibriophage, kvp40, was isolated from sea water by using vibrio parahaemolyticus 1010 (eb101) as the indicator host. the host range of kvp40 extended over at least 8 vibrio and 1 photobacterium species. kvp40 was a large tailed phage containing double-stranded dna and belonged to ackermann's morphotype a2. kvp40 dna was cleaved by 11 different type ii restriction endonucleases including ecori and hindiii, but not by 17 other enzymes including bamhi, kpni and sali.19921584076
further study on polyamine compositions in vibrionaceae.it has previously been reported that norspermidine, one of the unusual polyamines, is present in vibrio species. to expand this observation, the cellular polyamine compositions of additional species and strains in the family vibrionaceae (vibrio, photobacterium, listonella, and shewanella) as well as aeromonas species and plesiomonas shigelloides, which have been proposed to be excluded from vibrionacea, were determined by using gas-liquid chromatography. some vibrio species previously reported ...19912059921
evaluation of the genus listonella and reassignment of listonella damsela (love et al.) macdonell and colwell to the genus photobacterium as photobacterium damsela comb. nov. with an emended description.the genus listonella, which was recently described on the basis of 5s rrna sequence data, was found to be of dubious value on the basis of the results of a comparison of a number of taxonomic studies involving members of the vibrionaceae. the available data suggest that 5s rrna sequences may be of limited taxonomic use at the intra- and intergeneric levels, at least for apparently recently evolved groups, such as the vibrionaceae. in this light, we assessed the generic assignment of the species ...19911742198
crystallization and preliminary x-ray diffraction studies of a flavoprotein, fp390, from a luminescent bacterium, photobacterium phosphoreum.a flavoprotein, fp390, obtained from a luminescent bacterium, photobacterium phosphoreum, in the purification of luciferase has been crystallized by the vapor-diffusion procedure. crystals obtained from polyethylene glycol 4000 solutions, whose x-ray photographs show powder diffraction patterns, were unsuitable for further crystallographic work. however, tetragonal crystals grown from potassium phosphate solution well diffracted x-rays beyond 3 a resolution. the space group of this crystal is p4 ...19911783606
deaths in captive eels (anguila reinhardtii) due to photobacterium (vibrio) damsela. 19921530562
sediment pore water toxicity identification in the lower fox river and green bay, wisconsin, using the microtox assay.microtox assays with two different methods of osmotic adjustment were used to assess the toxicity of pore waters from 13 sediment samples collected from the fox river watershed in wisconsin. no toxicity was observed in microtox assays osmotically adjusted with nacl; however, 15-min ec50 values for assays osmotically adjusted with sucrose ranged from 52 to 63% pore water. un-ionized ammonia accounted for a large part of the observed toxicity, but, based on a toxic units approach, did not account ...19921376238
microtox ec50 values for drinking water by-products produced by ozonolysis.the aim was to determine the microtox ec50 values of some aliphatic aldehydes and carboxylic acids of normal chain length with 1-14 carbon atoms since these compounds have been detected as ozonolysis by-products in drinking water. the aqueous ec50 values decreased with increasing chain length except for formaldehyde and for the c1-c7 acids. at chain lengths above c7, where methanolic saline solutions were utilized to promote solubility, the aldehydes were more toxic than their corresponding carb ...19921376239
crystallization of photobacterium leiognathi non-fluorescent flavoprotein, an unusual flavoprotein with limited sequence identity to bacterial luciferase.single crystals of the non-fluorescent flavoprotein (nfp) purified from photobacterium leiognathi strain s1 have been grown from ammonium sulphate solutions using the hanging drop vapour diffusion technique. the crystals grow as thin (0.06 mm) plates and belong to the orthorhombic space group c222(1): a = 57.06(3) a, b = 92.41(6) a, c = 99.52(6) a. there is one nfp monomer per asymmetric unit and crystals diffract to 2.2 a spacings on film. a complete native data set to 2.5 a resolution has been ...19921560468
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