TitleAbstractYear(sorted ascending)
[yersiniosis]. 19892752462
identification and characterization of an 8-kd peptide stimulating late erythropoiesis.chronic renal failure is often associated with severe anemia, subnormal levels of erythropoietin (epo), and the presence of erythropoietic inhibitors. we studied an anephric patient with polycystic kidney disease maintaining a hemoglobin level of 13-14 g. biochemical, endocrine, and hematological parameters were all within normal limits. epo levels examined on two occasions during the clinical investigation were found to be low to subnormal. plasma of this patient supported human bone marrow ery ...19892753086
in vitro evidence for an inhibitor of lipogenesis in serum from overfed obese rats.involvement of a humoral agent in regulation of energy balance has been demonstrated by parabiosis experiments. overfed obese rats produce a blood-borne factor that inhibits adipose fatty acid synthesis in their partners, resulting in loss of body fat without significant inhibition of food intake. an in vitro bioassay was developed to test small serum samples for antilipogenic activity. epididymal adipocytes from ad libitum-fed rats were preincubated for 12 h with 2% serum. basal adipocyte fatty ...19892764157
death due to disruption of sternal fixation wire--a rare complication after open heart surgery through sternotomy: report of a case.following sternotomy for open heart surgery, a 57-year-old man had the sternum closed in the usual manner with six no.22 gauge stainless steel wire sutures. twenty-four days later, sternum disruption occurred with only one wire suture in place, 3 wire sutures cutting through the bone, and 2 wire sutures fractured. a lacerating injury over the anterior surface of the right ventricle was rendered by these 2 fractured wires followed by fatal outcome. to our knowledge, death induced by fractured wir ...19892794943
[the effect of progesterone, testosterone and estradiol on steroid hormone release by the ovarian follicles of cows in vitro]. 19892806660
developmental cycle-specific host-free rna synthesis in chlamydia spp.the incorporation of radiolabeled gtp into rna in host-free chlamydia trachomatis serovar l2 organisms was investigated. the incorporation was partially inhibited by rifampin and dactinomycin and hydrolyzed by rnase. rna made by host-free chlamydiae consisted mainly of species of fewer than 800 bases in size, although 16s and 23s species were noted by agarose-gel electrophoresis. the hybridization of radiolabeled host-free rna to restriction fragments of the gene encoding the major outer membran ...19901698176
monoclonal antibodies to quebec strain (q17) of bovine rotavirus.fourteen monoclonal antibodies (mabs) against "quebec" strain (q17) of bovine rotavirus were isolated and characterized. four were specific for viral protein vp7 and ten were specific for viral protein vp6. five different isotypes were represented by this group of antibodies. all of the anti-vp6 and none of the anti-vp7 antibodies were sensitive to the effects of periodate on their antigen. the antibodies could be separated into three groups based on their relative resistance to the dissociation ...19901698323
production of enterotoxin and cytotoxin by aeromonas veronii.fourteen strains of aeromonas veronii were isolated from salt and fresh water in rio de janeiro and investigated for the presence of virulence factors. eleven (79%) a. veronii strains were positive for enterotoxin by the suckling-mouse test and thirteen (93%) produced hemolysin. of the 13 a. veronii strains that produced hemolysin, seven were investigated for cytotoxin production using vero cells as indicator cells and all of them were positive.19902095293
toxicology of selected pesticides, drugs, and chemicals. organophosphorus and carbamate insecticides.organophosphorus and carbamate insecticides produce acute toxicosis via a common mechanism--the inhibition of acetylcholinesterase--and, therefore, these categories of insecticides are considered together. this article discusses the mechanisms, toxicity, clinical signs, lesions, and diagnosis of, as well as treatment for, toxicoses produced by these compounds.19902180181
retinal neurite growth on astrocytes is not modified by extracellular matrix, anti-l1 antibody, or oligodendrocytes.two factors that may influence the course of axonal regeneration in the central nervous system (cns) are extracellular matrix (ecm) and cell surface molecules that may enhance or inhibit neurite outgrowth. whereas cultured astrocytes have been reported to be a good substratum for neurite outgrowth, there is recent evidence that cultured oligodendrocytes are inhibitory. to test the influences of 1) ecm components, 2) the l1 adhesion molecule, and 3) the inhibitory potential of mature oligodendroc ...19911828788
gas-liquid chromatographic analysis of cellular fatty acid methyl esters in aeromonas species.the cellular fatty acids of 39 strains belonging to the genus aeromonas (aeromonas hydrophila, aeromonas caviae, aeromonas sobria, aeromonas media, aeromonas schubertii, aeromonas veronii) were determined by high resolution gas-liquid chromatography. the fatty acid profiles were characterized by major amounts (60% or more) of one saturated (hexadecanoic acid = 16:0) and two unsaturated (hexadecenoic acid = 16:1 and octadecenoic acid = 18:1) acids. while the majority of the strains of the six spe ...19911930556
biochemical and genetic characterization of autoagglutinating phenotypes of aeromonas species associated with invasive and noninvasive disease.the genetic characteristics and biochemical and structural properties of a number of autoagglutinating (aa) strains of aeromonas associated with invasive and noninvasive disease in humans and infections in animals and from environmental sources were investigated. of 27 strains analyzed by multilocus enzyme typing and dna hybridization studies, 25 (93%) were confirmed to belong to either hybridization group 1 (phenospecies and genospecies aeromonas hydrophila) or 8 (phenospecies aeromonas sobria; ...19912010642
aeromonas jandaei and aeromonas veronii dual infection of a human wound following aquatic exposure.exudate removed from an infection that developed below the left eye of a 10-year-old male following a previously inflicted wound after aquatic exposure was cultured and revealed two different aeromonas spp. further characterization showed that one strain was phenotypically identical to aeromonas veronii, while the other strain was confirmed by dna hybridization analysis to be aeromonas jandaei sp. nov. this is the first report of these more recently described aeromonads, thus far rarely reported ...19912037674
[a strain of aeromonas veronii isolated from a patient suspected of cholera].investigations were carried on a strain isolated from patient hospitalized in poland. the patient returned from odessa, a region with cholera epidemic. the strain was suspected to be vibrio cholerae, but it did not agglutinate in v. cholerae 01 serum. the aim of this study was establishment of taxonomical classification of this strain, in order to demonstrate whether it is a representative of the non-cholera vibrio group (not agglutinating) or another representative of the family vibrionaceae. t ...19921297030
5-hydroxytryptamine receptor activity of the dopamine receptor agonist fenoldopam in canine tracheal smooth muscle.fenoldopam is a new vasodilator undergoing clinical trials for the treatment of hypertensive emergencies. its pharmacologic effects result from activation of vascular dopamine-1 receptors. in canine tracheal smooth muscle strips, fenoldopam caused a concentration- and calcium-dependent increase in tension, which was not antagonized by atropine, indomethacin or the dopamine-1 receptor antagonist, sch 23390. the ec50 (1.89 x 10(-6) m) exceeded that of serotonin or acetylcholine (8.38 x 10(-8) and ...19921346635
[h2-receptor antagonist-refractory ulcer--its pathophysiology and role of proton-pump inhibitors].among h2-receptor antagonist (h2ra)-refractory ulcers, non-responders that did not heal after 5 months therapy had high intraluminal ph in the basal condition and high sensitivity to inhibition of acid secretion by h2ra but possessed gastric mucosa to generate less prostaglandins. combination therapy of pge1-analogue with h2ra healed these ulcers by 60%. proton-pump inhibitor (ppi) exerted a complete inhibition of acid secretion in these patients and the rate of healing was 88%. helicobacter pyl ...19921347325
antigenic diversity of the s-layer proteins from pathogenic strains of aeromonas hydrophila and aeromonas veronii biotype sobria.the antigenic relatedness of paracrystalline surface array proteins with subunit molecular weights of approximately 52,000 from isolates of aeromonas hydrophila and aeromonas veronii biotype sobria belonging to a single heat-stable serogroup was examined. enzyme-linked immunosorbent assay and immunoblotting with two different polyclonal antisera against surface exposed and non-surface-exposed epitopes of the s-layer protein from a. hydrophila tf7 showed that the s-layer proteins of the mesophili ...19921370287
immunochemical analysis and possible biological role of an aeromonas hydrophila surface array protein in septicaemia.the biochemical, immunological, and biological properties of an s layer purified from an aeromonas hydrophila strain (ah-342) involved in a case of bacteraemia were investigated. the s layer selectively removed from the cell surface was composed of a single acidic (pi 4.56) protein subunit (surface array protein, sap) with a molecular mass of approximately 52 kda. amino acid analysis of this 52 kda protein indicated a molecule composed of 498 amino acids with 46% hydrophobic residues. no cystein ...19921382113
halogenated isoniazid derivatives as possible antitubercular and antineoplastic agents. note 1.halogenated arylhydrazones, 2-aryl-4-thiazolidinones and their 1,1-dioxides containing isoniazid (ini) moieties were prepared and explored for antimicrobial (against bacteria, candida and mycobacteria) and antitumor activities. none of the tested compounds showed any marked antimicrobial effect. furthermore, among the compounds submitted to nci in vitro primary antitumor screening, those bearing 3-fluoro or -chlorophenyl substituents were found to possess selective inhibitory effects on the grow ...19921388607
thermally-induced delayed fluorescence of photosystem i and ii chlorophyll in thermophilic cyanobacterium synechococcus elongatus.stationary delayed fluorescence (df) of chlorophyll in isolated membrane preparations from thermophilic cyanobacterium synechococcus elongatus was investigated as a function of temperature. two peaks at different temperatures were observed. the low-temperature peak (54-60 degrees c) coincided with the main maximum of the thermally-induced delayed fluorescence of chlorophyll in intact cells and psii-particles with active oxygen-evolving system. the high-temperature peak (78 degrees c) coincided w ...19921426973
pili and the interaction of aeromonas species with human peripheral blood polymorphonuclear cells.the interaction of differentially piliated aeromonas strains expressing pili of two broadly different morphologic types (short, rigid (s/r) and/or long, wavy (l/w)) with human peripheral blood mononuclear leukocytes (pmn) was investigated to determine whether host defense cells might exert a selective pressure on pili expression in vivo accounting for the different pili phenotypes of clinical and environmental strains. a majority of aeromonas veronii biotype sobria strains from water (6/6) and f ...19938104626
meningitis due to aeromonas species: case report and recent years, aeromonas species has been reported to cause extraintestinal infections with a growing frequency. meningitis due to aeromonas species is, however, a rare entity. we report a case of aeromonas meningitis in a 54-year-old man with a history of chronic alcoholic liver disease who, after an episode of gastroenteritis, developed an acute clinical picture characteristic of meningitis with septic shock and ecthyma gangrenosum. aeromonas veronii (biogroup sobria) was isolated from cultu ...19938110931
aeromonas enteropelogenes and aeromonas ichthiosmia are identical to aeromonas trota and aeromonas veronii, respectively, as revealed by small-subunit rrna sequence analysis.the 16s rrna gene sequences of the type strains of aeromonas enteropelogenes and aeromonas ichthiosmia were determined by polymerase chain reaction direct sequencing in order to clarify their interrelationships with other aeromonad species. on the basis of 16s rrna gene sequence analysis, a. enteropelogenes and a. ichthiosmia were found to be identical to aeromonas trota and aeromonas veronii, respectively.19938240968
identification of aeromonas species isolated from freshwater fish with the microplate hybridization method.aeromonas isolates were obtained from the intestinal tracts of six species of cultured freshwater fish and identified on the basis of their genotypic and phenotypic characters. the microplate hybridization method could differentiate type strains of aeromonas species and related bacteria. dna-dna hybridization analysis showed that 65 aeromonad isolates were 72 to 100% related with either aeromonas caviae, aeromonas hydrophila, aeromonas jandaei, aeromonas sobria, or aeromonas veronii. as many as ...199416349363
case of aeromonas veronii (dna group 10) bacteremia.we describe the first case report of bacteremia due to aeromonas veronii biotype veronii. the infection occurred in a 77-year-old man suffering from multiple underlying conditions which included cancer of the sigmoid colon. because of the unusual biochemical phenotype of this group (ornithine decarboxylase positive), it was originally identified as vibrio cholerae.19947883912
aeromonas species in septicemia: laboratory characteristics and clinical observations.we retrospectively analyzed clinical and epidemiological data on and laboratory characteristics of 53 cases of aeromonas septicemia. only four aeromonas genomospecies (species defined by dna relatedness) were associated with the 53 cases, with aeromonas hydrophila (sensu stricto) predominating (47%). nearly 60% of all aeromonas isolates from blood fell into one of four somatic groups: serogroups o:11, o:16, o:18, and o:34. unlike aeromonas-associated gastroenteritis, septicemia did not peak in f ...19947948561
the susceptibility of s-layer-positive and s-layer-negative aeromonas strains to complement-mediated lysis.forty strains of aeromonas hydrophila and aeromonas veronii recovered from invasive and non-invasive infections were tested for their susceptibility to complement-mediated lysis by 65% pooled human serum (phs). based upon the results of this assay, two major populations could be defined. the first group (n = 20) consisted of serogroup o:11 strains, all of which possessed a paracrystalline surface layer (s layer); all of these strains were refractory to the bactericidal activity of 65% phs with t ...19948000552
constant and variable regions in glycoprotein hormone beta subunit sequences: implications for receptor binding specificity. 1994201763
distribution of alginate genes in bacterial isolates from corroded metal surfaces.the distribution of alginate genes encoding biosynthesis of alginate was examined for bacterial isolates associated with corrosive biofilms recovered from source water, cooling lines, and reactor surfaces of a nuclear power plant. a total of 120 diverse gram-positive and -negative isolates were obtained. using dna:dna hybridization, 11 isolates were shown to contain sequences homologous to structural (algd, algg, alg-76) and/or regulatory (albb) alginate biosynthetic genes derived from an algina ...199424190336
conformational energy calculations for dinucleotide molecules. a systematic study of dinucleotide conformation, with application to diadenosine pyrophosphate. 1995177120
electron microscopic examination of factors influencing the expression of filamentous surface structures on clinical and environmental isolates of aeromonas veronii biotype sobria.strains of aeromonas veronii biotype sobria isolated from clinical and environmental sources were examined for their expression of surface structures under a variety of culture conditions. when grown on solid media at 37 degrees c, more than 95% of bacteria from the majority of strains isolated from human diarrheal feces and chicken carcasses were non-piliated or expressed only a few pili of long, flexible morphology per cell. strains isolated from water or other foods were much more likely to e ...19957565173
a specific puld homolog is required for the secretion of paracrystalline surface array subunits in aeromonas hydrophila.aeromonas hydrophila is an important pathogen of fish, and its high-virulence strains display a two-dimensional paracrystalline layer (s-layer) on their outermost surfaces. the nucleotide sequence of a 4.1-kb region located 700 bp upstream of the a. hydrophila tf7 s-layer protein gene (ahsa) has been determined. a sequence analysis of the region revealed the presence of three complete open reading frames ending in a gene encoding a 79.8-kda polypeptide that shows high homology to the puld family ...19957608063
[new species of aeromonas isolated in cuba].one hundred and fifty-five strains of aeromonas isolated in the stools of children under 5 years presenting with acute diarrheal disease were studied. using the aerokey ii system for the identification of species, 47 strains were identified as aeromonas caviae, 58 as aeromonas hydrophila, 23 as aeromonas veronii biovar sobria, 14 as aeromonas trota, 9 as aeromonas veronii biovar veronii, 2 as aeromonas jandaei and 2 as aeromonas shubertii, emphasis is placed on the advantages of this method whic ...19959813480
adhesion of aeromonas sp. to cell lines used as models for intestinal adhesion.adhesion to hep-2 cells has been shown to correlate with enteropathogenicity for aeromonas species. such adhesion is thought to reflect the ability of strains to adhere to human intestinal enterocytes, although hep-2 cells are not of intestinal origin. in this study strains of aeromonas veronii biotype sobria isolated from various sources were investigated in parallel assays for their ability to adhere to hep-2 cells and to an intestinal cell line (caco-2). quantitative assays showed identical a ...19958557078
distribution of aeromonas species in the intestinal tracts of river fish.aeromonas isolates were obtained from fish intestines, water, and sediments from an urban river and identified by the dna-dna microplate hybridization method. the isolates were aeromonas veronii (22%), aeromonas caviae (18%), aeromonas hydrophila (13%), aeromonas sobria (8%), aeromonas jandaei (7%), and other aeromonas spp. (33%). aeromonas species occurred at high densities with high incidences, regardless of season. the results strongly suggest that aeromonads are indigenous in fish intestines ...199516535171
synergistic iron reduction and citrate dissimilation by shewanella alga and aeromonas veroniitwo bacterial isolates from great bay estuary, new hampshire, in co-culture carried out anaerobic dissimilation of citric acid with fe(iii) as the terminal electron acceptor. neither isolate oxidized citrate with fe(iii) anaerobically in axenic culture. the fe(iii) reducer, shewanella alga strain bry, did not grow anaerobically with citrate as an energy source. the citrate utilizer, aeromonas veronii, did not reduce iron axenically with a variety of electron donors including citrate. the onset o ...19968824150
characterization of a type iv bundle-forming pilus (sfp) from a gastroenteritis-associated strain of aeromonas veronii biovar sobria.the colonization mechanisms of enteropathogenic aeromonas strains are poorly characterized, but recent studies indicate that some filamentous structures are intestinal adhesins. this study describes the purification and characterization of a long, flexible pilus from a gastroenteritis-associated strain of aeromonas veronii biovar sobria. sds-page analysis (various conditions) of pili preparations yielded a pilin protein band of approximately 21 kda. its n-terminal amino acid sequence was unambig ...19968827704
high-resolution genotypic analysis of the genus aeromonas by aflp fingerprinting.we investigated the ability of a recently developed genomic fingerprinting technique, named aflp, to differentiate the 14 currently defined dna hybridization groups (hgs) in the genus aeromonas. we also determined the taxonomic positions of the phenospecies aeromonas allosaccharophila, aeromonas encheleia, aeromonas enteropelogenes, and aeromonas ichthiosmia, which have not been assigned to hgs yet. a total of 98 aeromonas type and reference strains were included in this study. for the aflp anal ...19968934910
identification and molecular characterization of two tandemly located flagellin genes from aeromonas salmonicida a449.two tandemly located flagellin genes, flaa and flab, with 79% nucleotide sequence identity were identified in aeromonas salmonicida a449. the fla genes are conserved in typical and atypical strains of a. salmonicida, and they display significant divergence at the nucleotide level from the fla genes of the motile species aeromonas hydrophila and aeromonas veronii biotype sobria. flaa and flab encode unprocessed flagellins with predicted mrs of 32,351 and 32,056, respectively. when cloned under th ...19979286979
distribution and expression of beta-lactamase genes among aeromonas spp.clinical and environmental isolates of aeromonas spp. (20 aeromonas caviae, 33 aeromonas veronii and 21 aeromonas hydrophila) were examined for their ability to yield mutants derepressed for beta-lactamase production and for the distribution of the three chromosomally encoded beta-lactamase genes, amps and ceps from a. veronii bv. sobria and cpha from a. hydrophila. of these isolates, 100% and 96% of the isolates from a. hydrophila and a. caviae, respectively, yielded beta-lactamase derepressed ...19979301981
indwelling device-related and recurrent infections due to aeromonas species.from october 1995 to february 1997, 13 isolates of aeromonas species were recovered from four patients treated at national taiwan university hospital (taipei). one of the patients, a diabetic, had simultaneous aeromonas veronii biotype veronii bacteremia and a. veronii biotype sobria urinary tract infection. seven weeks after the episode, the patient had necrotizing fasciitis due to a. veronii biotype veronii. the other three patients all had underlying hepatobiliary malignancies complicated by ...19989524839
nucleotide and amino acid sequences of the metallo-beta-lactamase, imis, from aeromonas veronii bv. sobria.the aeromonas veronii bv. sobria metallo-beta-lactamase gene, imis, was cloned. the imis open reading frame extends for 762 bp and encodes a protein of 254 amino acids with a secreted modified protein of 227 amino acids and a predicted pi of 8.1. to confirm the predicted sequence, purified imis was digested and the resulting peptides were identified, yielding an identical sequence for imis, with 98% identity to cpha. both possessed the putative active-site sequence asn-tyr-his-thr-asp at positio ...19989527802
misidentification of unusual aeromonas species as members of the genus vibrio: a continuing problem.two unusual cases of aeromonas infection are described, one associated with bacteremia (aeromonas schubertii) and another in which the organism was recovered from an infected gall bladder (aeromonas veronii biotype veronii). these strains were initially identified as vibrio damsela and vibrio cholerae by the vitek and api 20e systems, respectively. use of appropriate screening tests and familiarity with the newer aeromonas species could prevent initial misidentifications and potential public hea ...19989542946
phenotypic identification of aeromonas genomospecies from clinical and environmental sources.a collection of 983 aeromonas isolates from environmental and clinical sources have been identified to the genomospecies level. a phenotypic method identified 93% of the strains. the use of citrate and the production of acid from sorbitol enabled the members of the aeromonas hydrophila complex to be separated. the most common genomospecies from intestinal sources were aeromonas veronii biotype sobria and aeromonas caviae. the former, together with a. hydrophila, was the most frequently isolated ...19989575026
septic arthritis due to aeromonas veronii biotype sobria. 19989709899
the production of a novel carbapenem-hydrolysing beta-lactamase in aeromonas veronii biovar sobria, and its association with imipenem resistance. 199810052912
prevalence, in-vitro secretory activity, and cytotoxicity of aeromonas species associated with childhood gastroenteritis in chennai (madras), investigation on the prevalence of aeromonas in gastrointestinal illnesses of pediatric inpatients 1 month to 3 years of age was conducted from february 1997 through january 1998 in madras. sixteen aeromonas spp. were isolated from 11 male and five female children among the 341 pediatric inpatients suffering from acute diarrhoea. a. caviae, which was isolated from nine cases, was found to be the most predominant isolate, followed by a. veronii biovar sobria, isolated from six cases, and a. hy ...199810211427
health expenditure 1978/79. 1998104698
cellular changes in the toad urinary bladder in response to metabolic acidosis.the urinary bladder of bufo marinus excretes h+ and nh+4, and the h+ excretion is increased when the animal is placed in metabolic acidosis. the mitochondria-rich (mr) cells mediate the h+ excretion by the bladder. the purpose of this study was to determine if there is a change in mr cells of the bladder during metabolic acidosis. bladders from normal toads and from toads that had been placed in metabolic acidosis were used. the bladders were mounted between plastic chambers and h+ excretion mea ...199996269
antimicrobial activity of essential oils and other plant extracts.the antimicrobial activity of plant oils and extracts has been recognized for many years. however, few investigations have compared large numbers of oils and extracts using methods that are directly comparable. in the present study, 52 plant oils and extracts were investigated for activity against acinetobacter baumanii, aeromonas veronii biogroup sobria, candida albicans, enterococcus faecalis, escherichia col, klebsiella pneumoniae, pseudomonas aeruginosa, salmonella enterica subsp. enterica s ...199910438227
diverse restriction fragment length polymorphism patterns of the pcr-amplified 16s rrna genes in aeromonas veronii strains and possible misidentification of aeromonas species.restriction fragment length polymorphism analysis after pcr amplification (rflp-pcr) of the 16s rrna gene has been previously proposed as a rapid method to identify aeromonas species. in the present study, the precision of rflp-pcr was evaluated with 62 aeromonas reference strains. the analysis revealed that aeromonas veronii biovar sobria strains produce various patterns, possibly leading to its misidentification as an environmental species. for most other aeromonas species little variation was ...199910488176
functional characterization of type iv pili expressed on diarrhea-associated isolates of aeromonas species.our past work has shown that long, flexible type iv pili (single or in bundles) are the predominant pili expressed on fecal isolates of diarrhea-associated species of aeromonas (aeromonas veronii biovar sobria and a. caviae). they represent a family of type iv pili which we have designated bfp (for bundle-forming pili). reports from japan suggest that bfp are intestinal colonization factors. this study presents compelling evidence to support this conclusion. aeromonas bacteria and/or bfp purifie ...199910496928
virulence factors in aeromonas veronii bv veronii. 199910897894
symbiosis of aeromonas veronii biovar sobria and hirudo medicinalis, the medicinal leech: a novel model for digestive tract associations.hirudo medicinalis, the medicinal leech, is applied postoperatively in modern medicine. infections by aeromonas occur in up to 20% of patients unless a preemptive antibiotic treatment is administered. the associated infections demonstrate the need for a better understanding of the digestive tract flora of h. medicinalis. early studies reported the presence of a single bacterial species in the digestive tract and suggested that these bacteria were endosymbionts contributing to the digestion of bl ...19999864188
spontaneous bacterial empyema caused by aeromonas veronii biotype sobria.spontaneous bacterial empyema is a complication of hepatic hydrothorax in cirrhotic patients. the pathogen, clinical course and treatment strategy are different to the empyema secondary to pneumonia. a 54-year-old man, who was a cirrhotic patient with hepatic hydrothorax, was admitted to national taiwan university hospital for fever, dyspnea and right side pleuritic pain. the image study revealed massive right pleural effusion and no evidence of pneumonia. the culture of pleural effusion yielded ...200010974579
epidemiological relationships between aeromonas strains isolated from symptomatic children and household environments as determined by ribotyping.ribotyping was used to study the epidemiology of aeromonas associated gastro-enteritis in young children. ribotyping patterns of 29 aeromonas strains (16 aeromonas caviae, 8 aeromonas hydrophila, 3 aeromonas eucrenophila, 1 aeromonas veronii, and 1 aeromonas encheleia) isolated from primary stool cultures of sick children were compared using the gelcompare software with patterns of 104 strains (39 aeromonas eucrenophila, 29 aeromonas caviae, 11 aeromonas encheleia, 10 aeromonas hydrophila, 6 aer ...200010997832
production of heat-labile enterotoxin by strains of aeromonas veronii bv veronii.three isolates of aeromonas veronii bv veronii (2 environmental, one blood infection) were examined to see if they produce any enterotoxin and, if so, to determine its characteristics. two isolates caused fluid accumulation in the initial rabbit ileal loop tests. the other strain did so after a single passage through the rabbit ileal loop. all the isolates showed gradual enhancement of fluid secretion after each subsequent passage. inocula of 1 x 10(4) viable cells and 0.25 ml of culture filtrat ...200011014770
identification of genes associated with copper tolerance in an adhesion-defective mutant of aeromonas veronii biovar sobria.tnphoa mutagenesis was used to identify adhesins of aeromonas veronii biovar sobria 3767, a strain isolated from a diarrhoeal stool specimen. six mutants, from a library of 154, exhibited significantly reduced levels of adhesion to hep-2 cells. primers to the terminal regions of tnphoa were used for inverse pcr and the product from one mutant was cloned into pbluescript and partial sequence data obtained. scanning genbank and embl data bases revealed dna sequence similarity to the copa gene of p ...200011024350
production and secretion of collagen-binding proteins from aeromonas veronii.collagen-binding protein (cnbp) synthesized by aeromonas veronii is located conserved within the subcellular fraction. the results of this study show that 98% of the total cnbp produced by aer. veronii is present in the extracellular medium, and that the remaining cnbp is distributed either on the cell surface, within the periplasm or anchored on the outer membrane. cnbp is specifically secreted from aer. veronii into the culture medium, because all the beta-lactamase activity was located in the ...200011054164
ingested blood contributes to the specificity of the symbiosis of aeromonas veronii biovar sobria and hirudo medicinalis, the medicinal leech.hirudo medicinalis, the medicinal leech, usually carries in its digestive tract a pure culture of aeromonas veronii bv. sobria. such specificity is unusual for digestive tracts that are normally colonized by a complex microbial consortium. important questions for the symbiotic interaction and for the medical application after microvascular surgery are whether other bacteria can proliferate or at least persist in the digestive tract of h. medicinalis and what factors contribute to the reported sp ...200011055917
interaction between aeromonas veronii and epithelial cells of spotted sand bass (paralabrax maculatofasciatus) in in vitro fish model to study the interaction between aeromonas veronii and skin, gill and intestinal epithelial cells was developed using primary cultures of mucosal cells (isolated from healthy organisms). primary cultures were exposed to aeromonas veronii strain a186 isolated from a patient with severe gastrointestinal disease. microbial adherence was assessed by a spectrophotometric evaluation of an enzyme-linked, biotin-streptavidin aer. veronii cell-adhesion assay to confluent monolayers ...200010792551
anti-adhesive activity of sulphated exopolysaccharides of microalgae on attachment of red sore disease-associated bacteria and helicobacter pylori to tissue culture cells.because of the affinity of certain bacterial species for sulphated glycoconjugates exposed on the epithelial cells of susceptible hosts, we hypothesized that sulphated exopolysaccharides of microalgae can be used in anti-adhesive therapies against bacterial infections, both in cold- and warm-blooded animals. in this study we found that adhesion of the human pathogen helicobacter pylori to the hela s3 cell line, and adhesion of the fish pathogens vibrio campbellii, v. ordalii, streptococcus sapro ...200010849279
typing of clinical and environmental aeromonas veronii strains based on the 16s-23s rdna spacers.genetic relationships of aeromonas veronii strains isolated from human and environmental sources were investigated by restriction fragment length polymorphism (rflp) of the polymerase chain reaction-amplified intergenic spacer region (isr) flanked by the 16s and 23s rrna genes. when using endonucleases alui, hinfi and cfoi the 16s-23s rdna-rflp patterns showed considerable overall similarity, although most strains yielded specific profiles. several intra-specific lines of descent comprised clini ...200010865175
immunologic features of a carcinogen-induced murine bladder cancer: in vivo and in vitro studies.certain in vivo and in vitro immunologic features of carcinogen-induced murine bladder cancer have been studied. the consistency of tumor induction, its natural history, and immunogenicity both in vivo and in vitro render this syngeneic murine bladder tumor a suitable model for immunologic studies. pre-immunization of strain c3h/hen mice with mid-gestational fetal cells did not protect the animals from tumor challenge. sera of mice immunized with mid-gestational fetal cells were not cytotoxic to ...200086659
a new method for degradation of the protein part of glycoproteins: isolation of the carbohydrate chains of asialofetuin.a new method has been developed for degrading the protein part of several glycoproteins, whilst leaving the carbohydrate portion virtually intact apart from partial degradation at the reducing end. the method is based upon stabilization of the glycosidic linkages of the sugar residues by trifluoroacetyl groups and subsequent cleavage of the peptide bonds by transamidation. the two reactions are carried out in a mixture of trifluoroacetic anhydride and trifluoroacetic acid. after o- and n-detrifl ...200089909
[overview of the history of ceramics instead of a preamble]. 200190452
isolation and 16s rrna sequence analysis of the beneficial bacteria from the rhizosphere of rice.the present study deals with the isolation of plant growth promoting rhizobacteria (pgpr) from rice (variety niab irri-9) and the beneficial effects of these inoculants on two basmati rice varieties. nitrogen-fixing activity (acetylene-reduction activity) was detected in the roots and submerged shoots of field-grown rice variety niab irri-9. estimation of the population size of diazotrophic bacteria by ara-based mpn (acetylene reduction assay-based most probable number) in roots and shoots indic ...200111261489
mucosal immune response of spotted sand bass paralabrax maculatofasciatus (steindachner, 1868) orally immunised with an extracellular lectin of aeromonas assess the immunogenic and immunoprotective role of the extracellular lectin from aeromonas veronii (mcbp), which has affinity for mucosal constituents such as mucin, lactoferrin, immunoglobulins and collagen, spotted sand bass (paralabrax maculatofasciatus) were orally immunised either with soluble mcbp, adjuvant-conjugated mcbp or immobilised mcbp on latex microspheres. the results suggest that the mcbp is capable of eliciting protective immunity against a. veronii infections when administe ...200111308074
mesophilic aeromonads in wild and aquacultured freshwater fish.numbers and species of motile aeromonas were determined in freshly caught freshwater fish, in the surrounding environment, and also during iced chilled storage of fish specimens. although no significant differences were observed in water samples, initial levels for skin, gill, and intestines were significantly lower in farmed rainbow trout (oncorhynchus mykiss) than in wild brown trout (salmo trutta) and pike (esox lucius). during storage of wild specimens, naturally occurring aeromonads grew fa ...200111348001
new dna-dna hybridization and phenotypic data on the species aeromonas ichthiosmia and aeromonas allosaccharophila: a. ichthiosmia schubert et al. 1990 is a later synonym of a. veronii hickman-brenner et al. 1987.previously, a dna fingerprinting study based on amplified fragment length polymorphism (aflp) analysis has revealed a possible genotypic resemblance of the species aeromonas ichthiosmia and aeromonas allosaccharophila to aeromonas veronii (huys et al., int. j. syst. bacteriol. 46, 572-580 [19961). currently, two genotypically indistinguishable biovars are known to exist in the latter species, namely a. veronii biovar sobria and a. veronii biovar veronii. in the current study, new dna-dna hybridi ...200111518320
helicobacter pylori and other enteric bacteria in freshwater environments in mexico city.helicobacter pylori infection is common in the mexican population; however, sources, routes, and risk factors for infection as well as mode of transmission remain unclear.200111578764
three cases of bacteraemia caused by aeromonas veronii biovar sobria.septicaemia caused by aeromonas species is a life-threatening condition, arising primarily in immunocompromised patients, which has rarely been reported in scandinavia. herein we describe 3 cases of aeromonas sobria bacteraemia from denmark. all the patients were male and all 3 cases occurred during the summer. two patients had acute leukaemia and hiv infection, respectively, while the third patient had colorectal cancer diagnosed several years later. the clinical presentation in all patients wa ...200111669236
mechanism of action of beta-blockers in hypertension. 200114643
family life cycle resource center in the elementary school. 200198495
microbial populations of an upflow anaerobic sludge blanket reactor treating wastewater from a gelatin industry.the microbial populations of an upflow anaerobic sludge blanket reactor, used for treating wastewater from the gelatin industry, were studied by microbiological methods and phase-contrast and electron microscopy. microscopy examination of the sludge showed a complex mixture of various rod-shaped and coccoid bacterial pluslong filaments and verymobile curved rods. in addition free-living anaerobic ciliates and flagellates were also observed. the trophic group population observed in decreasing ord ...200111873883
in vitro antimicrobial susceptibility of clinical isolates of aeromonas caviae, aeromonas hydrophila and aeromonas veronii biotype sobria. 200211909850
incidence of toxic aeromonas isolated from food and human hundred and ninety four aeromonas isolates (99 from food and 95 from clinical sources) were analyzed as to the species involved and the toxins produced. of the clinical isolates of aeromonas, 29.4% were enterotoxigenic, 43.1% were hemolytic and 89% were cytotoxigenic. among the food isolates, 18.2% were enterotoxigenic, 17.1% were hemolytic and 72.7% were cytotoxigenic. aeromonas sobria and aeromonas veronii produced more enterotoxin and cytotoxin than the other isolates, whereas a. veronii ...200211934569
first documented case of aeromonas veronii biovar sobria infection in northern europe.the first scandinavian case of aeromonas veronii biovar sobria infection, an episode of peritonitis in a patient suffering from a cirrhotic liver and an earlier unrecognized appendiceal cancer, is described.200212030401
virulence markers in aeromonas hydrophila and aeromonas veronii biovar sobria isolates from freshwater fish and from a diarrhoea evaluate the public health significance of representative strains of two aeromonas spp., mainly from freshwater fish, on the basis of production of virulence-associated factors and presence of the haemolytic genes aera and hlya.200212174039
aeromonas culicicola sp. nov., from the midgut of culex quinquefasciatus.the taxonomic position was examined of three isolates, mtcc 3249t, sh and slh, from the midgut of female culex quinquefasciatus and aedes aegyptii mosquitoes. numbers of cells of these isolates increased 2000-fold after a blood meal of the mosquitoes. 16s rrna gene sequence analysis of the novel strains showed that they were highly homologous to strains of aeromonas. dna-dna hybridization studies showed that dna of strain mtcc 3249t was 96 and 88% similar to that of strains sh and slh, respectiv ...200212361279
study of the effect of the midgut bacterial flora of culex quinquefasciatus on the susceptibility of mosquitoes to japanese encephalitis virus.culex quinquefasciatus mosquitoes were collected from the field and microbial flora was isolated from their midgut. isolates belonging to 13 different genera were obtained. four most abundant isolates viz. pseudomonas sp., acinetobacterjunii, staphylococcus epidermis and aeromonas culicicola were used to study their effect on the susceptibility of mosquitoes to japanese encephalitis virus (jev). incorporation of individual isolates in the mosquito bloodmeal resulted in an increased susceptibilit ...200212693864
[dental findings in children with hearing disorders].tooth findings are reported of 972 children aged between 3 to 18 years who suffer from impaired hearing. logopedic pupils had significantly more tooth anomalies than those with impaired and normal hearing. the deaf pupils had significantly less dysgnathias than others with impaired hearing. as compared to those with normal hearing there was no significant difference.2002124119
identification and characterization of pathogenic aeromonas veronii biovar sobria associated with epizootic ulcerative syndrome in fish in bangladesh.sparse information is available on the virulence factors of aeromonas strains isolated from diseased fish, from the environment, and from humans. in the present study, 52 aeromonas isolates obtained from epizootic ulcerative syndrome (eus) lesions in fish, from the aquatic environment, and from children with diarrhea in bangladesh were identified by biochemical phenotyping (i.e., pheneplate [php] typing) and dna fingerprinting and then characterized with respect to certain putative virulence fac ...200211823203
pelvic displacement of a cardiac pacemaker following trauma. a case report.the rate migration of an implanted cardiac pacemaker from the subrectus area anterior to the peritoneum to the pelvis following a motor vehicle accident is reported. discovery of dislodgement was made by observing a marked increase of pacemaker impulse amplitude in the three standard limb leads as measured on an oscilloscope.2003152472
molecular cloning, sequencing and characterization of omp48, the gene encoding for an antigenic outer membrane protein from aeromonas clone, sequence and characterize the gene encoding the omp48, a major outer membrane protein from aeromonas veronii.200312694457
genetic linkage of the penicillinase gene, amp, and blrab, encoding the regulator of beta-lactamase expression in aeromonas spp.aeromonas hydrophila t429125, a human clinical isolate, possesses three coordinately inducible beta-lactamases encoded by amph (class d beta-lactamase), ceph (class c beta-lactamase) and imih (class b beta-lactamase). we report that upstream of amph there are two genes, blra and blrb, encoding a putative two-component regulatory system. pcr studies revealed the same blrab-amp gene arrangement in all aeromonas spp. isolates tested; namely, aeromonas veronii bv. sobria, aeromonas jandaei, aeromona ...200312746371
characterisation of aeromonas spp. isolated from frozen fish intended for human consumption in mexico.a total of 82 strains of presumptive aeromonas spp. were identified biochemically and genetically (16s rdna-rflp). the strains were isolated from 250 samples of frozen fish (tilapia, oreochromis niloticus niloticus) purchased in local markets in mexico city. in the present study, we detected the presence of several genes encoding for putative virulence factors and phenotypic activities that may play an important role in bacterial infection. in addition, we studied the antimicrobial patterns of t ...200312781953
complement resistance is essential for colonization of the digestive tract of hirudo medicinalis by aeromonas strains.from the crop of the medicinal leech, hirudo medicinalis, only aeromonas veronii bv. sobria can be cultured consistently. serum-sensitive a. veronii mutants were unable to colonize h. medicinalis, indicating the importance of the mammalian complement system for this unusual simplicity. complementation of one selected mutant restored its ability to colonize. serum-sensitive mutants are the first mutant class with a colonization defect for this symbiosis.200312839811
phylogenetic affiliation of aeromonas culicicola mtcc 3249(t) based on gyrb gene sequence and pcr-amplicon sequence analysis of cytolytic enterotoxin gene.we determined the gyrb gene sequences of all 17 hybridizations groups of aeromonas. phylogenetic trees showing the evolutionary relatedness of gyrb and 16s rrna genes in the type strains of aeromonas were compared. using this approach, we determined the phylogenetic position of aeromonas culicicola mtcc 3249(t), isolated from midgut of culex quinquefasciatus. in the gyrb based-analysis a. culicicola mtcc 3249(t) grouped with a. veronii whereas, it grouped with a. jandaei in the 16s rrna based tr ...200312866846
aeromonas veronii biovar veronii septicaemia and acute suppurative cholangitis in a patient with hepatitis b.gram-negative bacilli of the genus aeromonas are widespread in aquatic environments and can be responsible for human infections. although aeromonas extraintestinal and systemic infections have been reported with growing frequency in recent years, aeromonas septicaemia remains an uncommon finding, often associated with serious underlying disease and predominantly related to the species aeromonas hydrophila, aeromonas veronii biovar sobria and aeromonas caviae. here, a case of a. veronii biovar ve ...200312867570
misidentification of aeromonas veronii biovar sobria as vibrio alginolyticus by the vitek find the cause of misidentification of aeromonads when using the vitek system.200312969502
taxonomic study of sucrose-positive aeromonas jandaei-like isolates from faeces, water and eels: emendation of a. jandaei carnahan et al. 1992.fourteen sucrose-positive aeromonas jandaei-like isolates from fresh water and reared european eels were subjected to a polyphasic study to determine their taxonomic position. numerical taxonomy was used to analyse phenotypic data obtained for these isolates and 43 type and reference strains representative of recognized aeromonas species. the a. jandaei cluster (phenon 1) was defined at 81.6 % similarity (s(j)); this included the a. jandaei-like isolates, the sucrose-positive strain aeromonas ve ...200313130026
analysis of 16s-23s intergenic spacer regions and rrn operon copy number of aeromonas culicicola mtcc 3249t.the 16s-23s intergenic spacer and 23s rrna gene sequence were determined for a. culicicola mtcc 3249t. ten different isr, indicative of ten rrn operons, were found in the strain that were grouped in three major types. of the three types, isr i was non-coding while isr ii and iii coded for trna(glu)(uuc). the trna(glu)(uuc) sequence was identical to that of e. coli. both isr i and ii were represented by three clones whereas four clones represented isr iii. the number of nucleotide differences bet ...200314509830
molecular characterization and distribution of virulence-associated genes amongst aeromonas isolates from libya.the aim of the study was to type 52 aeromonas spp. isolates from chicken carcasses, children with diarrhoea and a hospital environment in libya, and to determine the distribution of putative virulence genes amongst them.200314633028
[a successful treatment of progressive necrotizing myositis caused by aeromonas veronii biotype sobria].we report a 52 year-old patient with malignant lymphoma who developed necrotizing soft tissue infection in the left lower extremity by aeromonas veronii biotype sobria during a neutropenic period after intensive chemotherapy. we decided to amputate the involved leg when signs and symptoms of necrotizing myositis were progressing despite administration of sensitive antibiotics. he survived with subsequent intensive supportive care. it is to note that even though the patient's neutrophil count is ...200314672014
factors influencing prognosis after myocardial infarction. 2003161872
reduction of microbial contamination from ultrasonic scalers. 2004356863
effect of high-energy feedings on low birthweight infants.a comparison of the effect of 100.5 kj/30 ml (24 kcal/30 ml) feedings with that of 84 kj/30 ml (20 kcal/30 ml) feedings on infants with a birthweight of 1500 g or less was made. no consistent benefits in growth occurred and there was a tendency for an increase in complications in infants fed the 100.5 kj/30 ml milk. hence the higher-energy feeding cannot be recommended as the routine feeding for low birthweight infants.2004470692
children's literature on death and dying. 2004375165
hemolytic and proteolytic activities of aeromonas hydrophila and aeromonas veronii biovar sobria in broth and salmon extract at different temperatures.expression of hemolytic and proteolytic activities throughout the growth cycle was investigated with two enterotoxic aeromonad strains assigned to the species aeromonas hydrophila and aeromonas veronii biovar sobria. although growth kinetic data were dependent on strain, temperature, and substrate, maximum populations attained were higher than 9 log cfu/ml in aerated tryptone soya broth plus yeast extract (tsbye) and salmon extract within the range 4 to 28 degrees c. for both strains in tsbye, v ...200414968959
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