| ethmoiditis with subperiosteal and retro-ocular abscesses due to aeromonas sobria in a 16-year-old boy exposed to the ardèche river. | | 2017 | 28343158 |
| post-translationally modified frog skin-derived antimicrobial peptides are effective against aeromonas sobria. | antimicrobial peptides (brevinin1 hyba1 and brevinin1 hyba2) identified from the skin secretion of an endemic frog species of western ghats were studied against fish pathogens. post-translational modifications such as c-terminal amidation and cyclization of the peptides were enhanced on the activity against aeromonas sobria. based on the minimum inhibitory concentration (3 μm), cyclic amidated brevinin hyba2 was identified as the most promising antimicrobial agent against a. sobria and can be de ... | 2017 | 28153544 |
| draft genome sequence of aeromonas sobria strain 08005, isolated from sick rana catesbeiana. | aeromonas sobria is a gram-negative, rod-shaped, and ubiquitous bacterium. we present here the draft genome sequence of a. sobria strain 08005, isolated from an infected bullfrog. it is composed of 66 contigs totaling 4,678,951 bp, contains 4,252 coding dna sequences (cdss), four rrnas, and 88 trna sequences, and shows the presence of various putative virulence-related genes. | 2017 | 28082483 |
| protective potency of clove oil and its transcriptional down-regulation of aeromonas sobria virulence genes in african catfish (clarias gariepinus l.). | disease episodes of fish caused by aeromonas species are moved to the top list of limiting problems worldwide. the present study was planned to verify the in vitro antibacterial activities as well as the in vivo potential values of clove oil and ciprofloxacin against aeromonas sobria in african catfish (clarias gariepinus). the in vitro phenotypic virulence activities and the successful amplification of aerolysin and hemolysin genes in the precisely identified a. sobria strain were predictive fo ... | 2016 | 27609474 |
| stress and humoral innate immune response of gilthead seabream sparus aurata cultured in sea cages. | innate and acquired immune responses of gilthead seabream sparus aurata was studied under normal culture and short-term stressful conditions for 18 months in offshore sea cages in alghero bay, italy. every 45 d, 50 fish were sampled and divided into two groups: fish in the first group (normal culture conditions) were bled after harvesting; fish in the second group were put into a tank under stressful conditions (crowding and confinement) and bled after 2 h. innate humoral immunity, such as compl ... | 2016 | 27485027 |
| a report of peritonitis from aeromonas sobria in a peritoneal dialysis (pd) patient with necrotizing fasciitis. | a 70-years of age, male patient with underlying type 2 diabetes mellitus, hypertension, dyslipidemia and ischemic heart disease had undergone continuous ambulatory peritoneal dialysis (capd)for 3 years without any episodes of peritonitis. he was diagnosed with necrotizing fasciitis and later developed peritonitis after receiving a laceration from an aquatic injury suffered during the flood disaster of 2011. the blood culture, necrotic tissue and the clear dialysate collected upon admission had s ... | 2015 | 27276849 |
| aeromonas sobria infection in farmed mud loach (misgurnus mizolepis) in korea, a bacteriological survey. | a disease outbreak occurred in june 2012 among mud loach cultured on pond farms in jangseong-gun, jeollanam-do, korea. mortality rates reached up to 1.2% in the farm per day. typical clinical signs were bleeding ulcer at the middle portion of head and haemorrhagic erosion of the operculum. based on biochemical characteristics, the causative bacterium isolated from diseased fish was identified as aeromonas sobria. the isolate expressed two haemolytic genes, aerolysin (sob) and haemolysin (asa1) g ... | 2015 | 27175175 |
| design of an immersion vaccine against aeromonad septicemia in perch (perca fluviatilis l.). | the production of an immersion vaccine and the vaccination procedure to immunize fry of perch (perca fluviatilis l.) against pathogenic aeromonas sobria that harbor a type iii secretion system is described. the vaccine, based on chemically inactivated a. sobria, enables rapid vaccination of a large number of fish by immersion of fry in an aqueous vaccine suspension during 5 min, giving them high protection during fattening under open water conditions in a freshwater lake for at least 4 months. | 2016 | 27076300 |
| effects of dietary acidifiers on growth, hematology, immune response and disease resistance of nile tilapia, oreochromis niloticus. | organic acids or their salts can be used as feed additives in aquaculture. this study was conducted to evaluate the use of a mixture of formic acid, propionic acid and calcium propionate compared with oxytetracycline (otc). a total of 720 apparently healthy oreochromis niloticus fingerlings with an average body weight of 28.8 ± 0.14 g (mean ± se) were randomly divided into four equal groups (g1, g2, g3and g4). g1 was fed a basal diet with no additive as the control group, while g2 and g3 were fe ... | 2016 | 26860238 |
| indolo[3,2-b]quinoline derivatives suppressed the hemolytic activity of beta-pore forming toxins, aerolysin-like hemolysin produced by aeromonas sobria and alpha-hemolysin produced by staphylococcus aureus. | in an attempt to discover inhibitory compounds against pore-forming toxins, some of the major toxins produced by bacteria, we herein examined the effects of four kinds of indolo[3,2-b]quinoline derivatives on hemolysis induced by the aerolysin-like hemolysin (alh) of aeromonas sobria and also by the alpha-hemolysin of staphylococcus aureus. the results showed that hemolysis induced by alh was significantly reduced by every derivative, while that induced by alpha-hemolysis was significantly reduc ... | 2016 | 26725434 |
| [aeromonas sobria infection in patients with acute leukemia:clinical features and outcome]. | to investigate the clinical features and outcome of patients with acute leukemia and aeromonas sobria infection. | 2015 | 26725395 |
| detection and characterization of potentially pathogenic aeromonas sobria isolated from fish hypophthalmichthys molitrix (cypriniformes: cyprinidae). | the current study focuses on the detection and characterization of potentially pathogenic aeromonas sobria from fish silver carp (hypophthalmichthys molitrix). assessment of clinical, microbiological, pathological and biochemical characteristics of a. sobria were taken into account in order to understand the epidemiology, frequency and occurrence of this infection. clinically the infected fish (h. molitrix) was observed for various types of symptoms. a total of 33 colonies of a. sobria strain we ... | 2016 | 26518124 |
| microbiological and parasitological investigation on chelonians reared in italian facilities. | the rapid rise in the number of pet chelonians and their illegal trade can modify the ecology, involving exotic pets, humans, and microbiological agents. therefore, different epidemiological situations and the related risk to introduce and spread infectious diseases, especially zoonotic agents, have to be considered. the aim of this study was to investigate the microbiological and parasitological situation in 2 chelonian facilities (a private breeding of tortoises and a shelter for turtles) coll ... | 2015 | 26344661 |
| structural basis for action of the external chaperone for a propeptide-deficient serine protease from aeromonas sobria. | subtilisin-like proteases are broadly expressed in organisms ranging from bacteria to mammals. during maturation of these enzymes, n-terminal propeptides function as intramolecular chaperones, assisting the folding of their catalytic domains. however, we have identified an exceptional case, the serine protease from aeromonas sobria (asp), that lacks a propeptide. instead, orf2, a protein encoded just downstream of asp, appears essential for proper asp folding. the mechanism by which orf2 functio ... | 2015 | 25784551 |
| aeromonas sobria necrotizing fasciitis and sepsis in an immunocompromised patient: a case report and review of the literature. | aeromonas veronii biovar sobria is a rare cause of bacteremia, with several studies indicating that this isolate may be of particular clinical significance since it is enterotoxin producing. a wide spectrum of infections has been associated with aeromonas species in developing countries that include gastroenteritis, wound infections, septicemia and lung infections. this infection, caused by aeromonas species, is usually more severe in immunocompromised than immunocompetent individuals. we here d ... | 2014 | 25245365 |
| antibacterial efficacy of recombinant siganus oramin l-amino acid oxidase expressed in pichia pastoris. | siganus oraminl-amino acid oxidase is a novel natural protein (named sr-laao) isolated from serum of the rabbitfish (s. oramin), which showed antibacterial activity against both gram-positive and gram-negative bacteria and had a lethal effect on the parasites cryptocaryon irritans, trypanosoma brucei brucei and ichthyophthirius multifiliis. in order to test whether recombinant sr-laao (rsr-laao) produced by the eukaryotic expression system also has antimicrobial activity, the yeast pichia pastor ... | 2014 | 25238719 |
| the manx shearwater (puffinus puffinus) as a candidate sentinel of atlantic ocean health. | seabirds have been historically used to monitor environmental contamination. the aim of the present study was to test the suitability of a species belonging to the procellariiformes group, the manx shearwater, puffinus puffinus, as a sentinel of environmental health, by determining contaminant levels (trace metals and organochlorine compounds) from carcass tissues and by isolating vibrio spp. and aeromonas spp. from live specimens. to this end, 35 puffinus puffinus carcasses wrecked on the north ... | 2014 | 25191536 |
| structure of the o-specific polysaccharide from the lipopolysaccharide of aeromonas sobria strain pt312. | the o-specific polysaccharide (ops) obtained by mild-acid degradation of the lipopolysaccharide from aeromonas sobria strain pt312 was studied by sugar and methylation analyses along with 1h and 13c nmr spectroscopy, including 2d 1h,1h cosy, tocsy, noesy, 1h-detected 1h,13c hsqc, and hmbc experiments. the sequence of the sugar residues was determined using 1h,1h noesy and 1h,13c hmbc experiments. it was found that the ops was built up of disaccharide repeating units composed of glcpnac and non-s ... | 2015 | 25037826 |
| cdna cloning and expression characterization of serum transferrin gene from oriental weatherfish misgurnus anguillicaudatus. | in this study, the cdna coding serum transferrin (stf) of misgurnus anguillicaudatus (mastf) was cloned. mastf cdna is composed of 2326 bp with a 2007 bp open reading frame encoding 668 amino acids. the deduced mastf protein consists of a signal peptide, two lobes (n and c-lobes) and signature motifs of transferrin (tf) family. the results of tissue distribution indicated that mastf mrna was predominantly expressed in the liver. the results indicate that the mastf expression increased significan ... | 2014 | 24673686 |
| molecular cloning and expression analysis of two β-defensin genes in the blunt snout bream (megalobrama amblycephala). | β-defensins are a group of cysteine-rich, cationic antimicrobial peptides that play important roles in innate immune system against pathogenic microbes invading. in this study, the part-length cdna sequences of two β-defensin genes (maβd-1, maβd-2) in blunt snout bream (megalobrama amblycephala) were identified. homology analysis showed that the cdna sequences of maβd-1 and maβd-2 had high similarities to those in common carp and zebrafish. real-time quantitative pcr results exhibited that expre ... | 2013 | 23876385 |
| adhesion to brown trout skin mucus, antagonism against cyst adhesion and pathogenicity to rainbow trout of some inhibitory bacteria against saprolegnia parasitica . | biological control of saprolegniosis with bacteria might be an alternative to the use of chemical compounds. among criteria for the selection of such bacteria are their absence of pathogenicity to fish and their ability to prevent adhesion of the pathogen to the skin mucus. the pathogenicity to rainbow trout of 21 bacterial isolates with in vitro inhibitory activity against saprolegnia parasitica was studied. fifteen of the isolates, identified as aeromonas sobria, pantoea agglomerans, pseudomon ... | 2013 | 23670078 |
| skin and soft-tissue infections caused by aeromonas sobria. | | 2013 | 23584005 |
| characterization of the bacterial microbiota of biomphalaria glabrata (say, 1818) (mollusca: gastropoda) from brazil. | roughly 200 000 000 people in 74 countries infected with schistosomes all share the fact that they came in contact freshwater harbouring infected snails. the aim of the study is to characterize the microbiota of wild and laboratory-reared snails of biomphalaria glabrata from pernambuco, brazil. the microbiota of these molluscs was identified biochemically by the vitek 2 automated microbiological system. antimicrobial susceptibility testing was carried out by the disc diffusion method with ß-lact ... | 2013 | 23488866 |
| detection of cyprinid herpesvirus 2 in association with an aeromonas sobria infection of carassius carassius (l.), in italy. | sixteen specimens of female crucian carp, carassius carassius (l.), during the breeding season, were investigated for post-mortem and full diagnostic examination during a mortality outbreak in a tributary stream of the arno river in tuscany in 2011. necropsy highlighted the presence of a swollen anus and widespread haemorrhages in the body, fins, gills and eyes. haemorrhages in internal organs and spleen granulomas were also observed. bacteria isolated from the brain, kidney and spleen of affect ... | 2013 | 23488630 |
| fulminant necrotizing fasciitis caused by aeromonas sobria in neutropenic patients. | infection with aeromonas species has been reported to occur in neutropenic patients. necrotizing fasciitis caused by aeromonas species is uncommon but potentially life-threatening. we herein describe three cases of fulminant necrotizing fasciitis caused by aeromonas sobria in neutropenic patients. these cases shared many clinical characteristics, including shock, coagulopathy, multiple organ failure and rapidly deteriorating and eventually fatal clinical courses. in all cases, aeromonas sobria w ... | 2012 | 23207126 |
| inhibition of aeromonas sobria serine protease (asp) by α2-macroglobulin. | asp is a serine protease secreted by aeromonas sobria. asp cleaves various plasma proteins, which is associated with onset of sepsis complications, such as shock and blood coagulation disorder. to investigate a host defense mechanism against this virulence factor, we examined the plasma for asp inhibitor(s). human plasma inhibited asp activity for azocasein, which was almost completely abolished by treating plasma with methylamine, which inactivates α2-macroglobulin (α2-mg). the asp-inhibitor co ... | 2012 | 23089609 |
| the tail nick augments aeromonas sobria serine protease (asp) activity in plasma through retarding inhibition by α2-macroglobulin. | asp is a serine protease secreted by aeromonas sobria, a sepsis-causing bacterium, and induces sepsis-mimicking disorders through plasma protein cleavage. the pathogen also secretes nasp that has a nick in the carboxy-terminal region. compared with single-chain asp (sasp), nasp had near-equivalent activity for small peptide substrates but was less proteolytic. surprisingly, nasp cleaved proteins more in plasma and was inhibited by human α(2)-macroglobulin more slowly than sasp. retarded inhibiti ... | 2012 | 22971340 |
| molecular cloning, recombinant expression and antibacterial activity analysis of hepcidin from simensis crocodile (crocodylus siamensis). | hepcidin, a cysteine-rich cationic antibacterial peptide, plays an important role in human defense against pathogen infection. however, its role in reptile immune response and whether it is involved in antibacterial immune have not yet been proven. in order to study the antibacterial activity of crocodylus siamensis hepcidin (cshepc), a common reptile which lives in topic region of southeast asia, a cdna sequence of cshepc was cloned, which included an open reading frame (orf) of 300 bp encoding ... | 2013 | 22967859 |
| production and properties of lipase of aeromonas sobria. | aeromonas have been isolated from a wide variety of aquatic environments. however the number of aeromonas in sea water is extremely small compared to that in fresh water. in in vitro culture, aeromonas can grow in mediums containing nacl at a concentration of 3.0%, this concentration corresponding to that of sea water. it is unclear why the number of aeromonas is low in sea water. exoproteins of bacteria are thought to be important for bacterial growth and survival in the environment. previously ... | 2012 | 22376235 |
| mortality of therapeutic fish garra rufa caused by aeromonas sobria. | to investigate a case of mass mortality of garra rufa (g. rufa) from a fish hatchery farm in slovakia. | 2012 | 23569873 |
| aeromonas sobria bacteriemia in an acute lymphoblastic leukemia case in remission. | | 2011 | 27263949 |
| aeromonas sobria in chlorinated drinking water supplies. | aeromonas species were recovered from over 27% of 183 chlorinated drinking water samples collected during an 18-month period. sixteen of 20 isolates tested elicited a cytotoxic response by y-1 mouse adrenal cells. none of the strains was either enterotoxigenic by the rabbit ligated ileal loop assay, exhibited piliation, or showed significant mannose resistant adherence to human buccal cells. theaeromonas isolates were further identified to bea. sobria and were resistant to ampicillin and suscept ... | 1982 | 24226050 |
| a truncated sph12-38 with potent antimicrobial activity showing resistance against bacterial challenge in oryzias melastigma. | antimicrobial peptides (amps) represent an efficient part of innate immunity and are found in a variety of life. among them histone 2a (h2a), as a promising class of amps, attracts great attention, but the in vivo mechanism of h2a derived amp is still less known. based on the acquisition of sphistin, a synthetic 38-amino acid h2a derived peptide from scylla paramamosain, as reported in our previous study, was truncated into three short fragments (sph12-38, sph20-38 and sph30-38) and further inve ... | 2017 | 28600196 |
| determination of spoilage microbiota of pacific white shrimp during ambient and cold storage using next-generation sequencing and culture-dependent method. | this study was conducted to determine the initial and spoilage microbiota of pacific white shrimp during ambient and cold storage using next-generation sequencing (ngs) and a culture-dependent method. the quality changes were also evaluated by sensory analysis and total volatile basic nitrogen (tvb-n) values. after 1 d of storage, the psychrotrophic bacteria were only 5.97 log cfu/g, accounting for 1.1% of the mesophilic bacteria counts (7.94 log cfu/g). the psychrotrophic bacteria counts exceed ... | 2017 | 28399332 |
| in vitro selection and identification of potential probiotics isolated from the gastrointestinal tract of nile tilapia, oreochromis niloticus. | fish gut bacteria can be used as probiotics for aquaculture. the aim of this study is to screen and identify beneficial probiotic bacteria from the gut of nile tilapia, oreochromis niloticus. nine out of one hundred thirty-five isolates were non-pathogenic through intraperitoneal injection and had antibacterial activities with at least a strain from the five isolated fish pathogens, aeromonas sobria, aeromonas hydrophila, pseudomonas aeruginosa, pseudomonas putida, and staphylococcus aureus. fur ... | 2017 | 28819794 |
| hematopoietic neoplasms accompanied by severe enterocolitis due to aeromonas species. | aeromonas species are known to be a cause of diarrhea and acute enterocolitis. however, only a few cases have been reported and the pathophysiology of aeromonas infection has not as yet been clarified. we experienced 2 cases developing severe enterocolitis during the course of hematological malignancies, specifically multiple myeloma and diffuse large b-cell lymphoma. both patients presented with watery diarrhea that persisted for more than a week, followed by bloody diarrhea. total colon endosc ... | 2017 | 28484157 |
| curcumin liposomes interfere with quorum sensing system of aeromonas sobria and in silico analysis. | aeromonas sobria is opportunistic pathogen frequently found in environment and food. interfering with its quorum sensing (qs) system could be a promising way to alleviate its virulence. in this study, curcumin liposomes were prepared and their characteristics like particle size, zeta potential, pdi (polymey disperse index), encapsulation efficiency and loading capacity were measured. the quorum sensing inhibitory effect of curcumin liposomes under sub-mic (minimum inhibitory concentration) on si ... | 2017 | 28819178 |
| efficacy of spirulina platensis diet supplements on disease resistance and immune-related gene expression in cyprinus carpio l. exposed to herbicide atrazine. | the present study evaluated the immunotoxicological effects of the herbicide atrazine (atz) at sub-lethal concentrations and the potential ameliorative influence of spirulina platensis (sp) over a sub-chronic exposure period on cyprinus carpio l., also known as common carp. common carp was sampled after a 40-days exposure to atz (428 μg/l) and sp (1%), individually or in combination to assess the non-specific immune response, changes in mrna expression of immune-related genes [lysozyme (lyz), im ... | 2017 | 28579523 |
| aeromonas sobria, an outbreak of natural infection in cultured silver catfish rhamdia quelen (quoy & gaimard, 1824) in argentina. | | 2017 | 28548705 |
| oxa-427, a new plasmid-borne carbapenem-hydrolysing class d β-lactamase in enterobacteriaceae. | to describe a novel plasmid-borne class d carbapenemase (chdl) named oxa-427 identified in several enterobacteriaceae clinical isolates from nine patients in one belgian hospital. | 2017 | 28859446 |
| strong genomic and phenotypic heterogeneity in the aeromonas sobria species complex. | aeromonas sobria is a mesophilic motile aeromonad currently depicted as an opportunistic pathogen, despite increasing evidence of mutualistic interactions in salmonid fish. however, the determinants of its host-microbe associations, either mutualistic or pathogenic, remain less understood than for other aeromonad species. on one side, there is an over-representation of pathogenic interactions in the a. sobria literature, of which only three articles to date report mutualistic interactions; on th ... | 2017 | 29276504 |
| involvement of the arg566 residue of aeromonas sobria serine protease in substrate specificity. | aeromonas sobria serine protease (asp) is an extracellular serine protease secreted by the organism. here, we identified the amino acid residue of asp that contributes to substrate specificity by using both synthetic peptides and biological protein components. the results showed that the arginine residue at position 566 (arg-566) of asp, which is located in the extra occluding region of asp close to an entrance of the catalytic cavity, is involved in the substrate specificity. a substitutional p ... | 2017 | 29023605 |
| secondary bacterial infections and antibiotic resistance among tungiasis patients in western, kenya. | tungiasis or jigger infestation is a parasitic disease caused by the female sand flea tunga penetrans. secondary infection of the lesions caused by this flea is common in endemic communities. this study sought to shed light on the bacterial pathogens causing secondary infections in tungiasis lesions and their susceptibility profiles to commonly prescribed antibiotics. participants were recruited with the help of community health workers. swabs were taken from lesions which showed signs of second ... | 2017 | 28886013 |
| aeromonas sobria serine protease (asp): a subtilisin family endopeptidase with multiple virulence activities. | aeromonas sobria serine protease (asp) is secreted from aeromonas sobria, a pathogen causing gastroenteritis and sepsis. asp resembles saccharomyces cerevisiae kex2, a member of the subtilisin family, and preferentially cleaves peptide bonds at the c-terminal side of paired basic amino acid residues; also accepting unpaired arginine at the p1 site. unlike kex2, however, asp lacks an intramolecular chaperone n-terminal propeptide, instead utilizes the external chaperone orf2 for proper folding, t ... | 2017 | 28432839 |