Publications

TitleAbstractYear
Filter
PMID
Filter
hypotensive effects after topical and intra-arterial administration of alpha-adrenergic agonists in isolated perfused rabbit eyes.the influence of nonselective and selective alpha-adrenergic agonists on intraocular pressure (iop) was studied in isolated arterially perfused rabbit eyes. drugs were given intra-arterially or topically. with either route of administration, no initial hypertensive phase was observed after l-epinephrine, l-norepinephrine, l-phenylephrine (alpha 1), or methoxamine (alpha 1), but there was an immediate ocular hypotensive effect. the alpha 2-agonist b-ht 920 caused a nonsignificant 1 mm hg fall in ...19852866480
changes in rat adrenal tyrosine hydroxylase mrna levels following chronic amphetamine administration. 19872888130
identification and characterization of mg2+-dependent phosphotyrosyl protein phosphatase from rat liver cytosol.although highly purified preparations of mg2+-dependent phosphoseryl protein phosphatase (also designated phosphatase ia or phosphatase 2c) dephosphorylated phosphotyrosyl histone, the activity has been resolved from phosphatase ia by polyacrylamide gel electrophoresis at ph 9.5. this novel phosphotyrosyl-specific protein phosphatase absolutely requires mg2+ or mn2+ for activity, is inhibited by zn2+, vanadate and fluoride, and has an optimal ph of 9.0 and mr = 50,000. certain properties of this ...19863022716
fever response in laboratory-maintained and free-ranging chuckwallas (sauromalus obesus).the fever response was studied in laboratory-maintained and free-ranging chuckwallas. five animals were used in a self-pairing laboratory experiment in which each animal was first injected with a sterile, pyrogen-free saline solution. approximately 7 days later, each individual was injected with a solution of 4.75 x 10(9) aeromonas sobria (formerly a. hydrophila). saline-injected individuals had a mean preferred body temperature (mpbt) of 36.8 +/- 0.4 (se) degrees c, whereas bacteria-injected an ...19892750958
production and characterization of monoclonal antibodies to aeromonas sobria surface antigens.a panel of eight murine monoclonal antibodies (mabs) was raised against the surface antigens of aeromonas sobria isolated from a patient with diarrhoea. antibodies to the lps core and o-antigen and to protein antigens were generated. three mabs of the igm isotype against either protein or lps agglutinated 34/75 aeromonas isolates from clinical and environmental sources. similar numbers of a. hydrophila, a. sobria and a. caviae isolates were agglutinated. the mabs were screened for their ability ...19892483346
fatal infection with aeromonas sobria and plesiomonas shigelloides. 19863081156
[aquatic contamination of wounds by aeromonas sobria after bathing in the river]. 19853160041
plasmids and resistance to antimicrobial agents in aeromonas sobria and aeromonas hydrophila clinical isolates.the antimicrobial susceptibilities of 75 aeromonas isolates were determined by agar dilution. differences in resistance patterns were observed between strains isolated from different geographic locations and between a. sobria and a. hydrophila isolates. multiple resistance was common; however, only one conjugative plasmid was detected. this 110-megadalton plasmid mediated resistance to eight antibiotics.19873631947
mutational specificity and the influence of excision repair: insights into the mechanisms of error-avoidance and error-fixation.the excision repair process controlled by the uvrabc gene in escherichia coli is the major pathway for the repair of a diverse series of dna damages. to achieve a better understanding of the mechanics of this repair pathway and its impact upon mutagenesis, we have applied a recently developed technology by which the nature of mutation is determined at the dna sequence level. a comparison of the classes and distribution of mutation in excision-repair-proficient and excision-repair-deficient strai ...19892698833
[the effect of progesterone, testosterone and estradiol on steroid hormone release by the ovarian follicles of cows in vitro]. 19892806660
enteropathogenicity of aeromonas species isolated from infants: a cohort study.the significance of aeromonas spp. as potential enteric pathogens was evaluated in a cohort of 187 infants aged 3-18 months during a 16-week summer period. aeromonas spp. were isolated from 14 of the 196 (7.1%) diarrhoeal episodes detected and from eight (5.2%) of 153 samples from paired asymptomatic infants. carriage of bacterial enteropathogens excluding aeromonas spp. was detected in a high proportion (23%) of the asymptomatic children. almost all of the seven isolates of aeromonas sobria wer ...19883216131
[toxic properties of lipopolysaccharide and lipid a of aeromonas sobria and non 01 vibrio cholerae]. 19883216765
[immunogenic and antigenic properties of bacterial cells, lipopolysaccharides and lipids a of aeromonas sobria and non 01 vibrio cholerae]. 19883216766
carbon substrate assimilation patterns of clinical and environmental strains of aeromonas hydrophila, aeromonas sobria and aeromonas caviae observed with a micromethod.the assimilation of carbon substrates by 103 strains of aeromonas of different origin identified by conventional methods was studied by means of a standardized micromethod containing 147 tests (api system). six distinct groups could be recognized and the discriminating substrates were determined. 3 species of aeromonas can be identified by means of conventional method: a. hydrophila, a. sobria and a. caviae. the method has a number of drawbacks: some media are unreliable, others are difficult to ...19883218392
stimulation of rabbit synoviocyte prostaglandin e2 synthesis by lipopolysaccharides and their subunit structures.lipopolysaccharides (lps) induce synoviocyte activation and may lead to destruction of synovial joint tissues. we assessed the production of prostaglandin e2 (pge2) as a measure of synoviocyte activation by lps and their subunit structures. diphosphoryl lipid a was the smallest portion of lipid a tested that stimulated pge2 production. the polysaccharide fraction of lps, containing the o antigen, was also active. intraarticular injections of the polysaccharide resulted in a synovitis very simila ...19883162365
aeromonas-associated gastroenteritis in children.during a 20-month period 55 strains of aeromonas species were isolated from 53 children with diarrhea. the isolation rate of 2.5% for aeromonas compared with the rates of 4.5% for shigella, 3.3% for salmonella, 2.7% for campylobacter and 0.05% for yersinia. in 45 children aeromonas was the sole bacterial enteropathogen identified. aeromonas was also isolated from 2 (0.5%) of 380 asymptomatic children. despite its known lack of identifiable virulence properties, aeromonas caviae was the most prev ...19883340460
prp (proline-rich protein) genes linked to markers es-12 (esterase-12), ea-10 (erythrocyte alloantigen), and loci on distal mouse chromosome 6.the closely linked proline-rich protein (prp) genes, coding for abundant salivary proteins, are located on distal mouse chromosome 6. they are part of a conserved linkage group that is represented on human chromosome 12p. two other markers, ea-10 and es-12, that were previously unassigned to a chromosome are closely linked to prp genes in the mouse.19892613230
mechanism of the epsp synthase catalyzed reaction: evidence for the lack of a covalent carboxyvinyl intermediate in catalysis.in order to detect covalent reaction intermediates in the 5-enolpyruvylshikimate-3-phosphate (epsp) synthase reaction, we have investigated the interaction of epsp synthase with the reaction product epsp. an exchange of epsp-methylene protons could be demonstrated by incubating epsps with epsp in d2o. since trace amounts of contaminating pi would lead to reversal of epsps reaction and hence methylene proton exchange, we added pyruvate kinase, adp, mg++ and k+. under these conditions, any contami ...19882838023
gas-liquid chromatographic analysis of fatty acid methyl esters of aeromonas hydrophila, aeromonas sobria, and aeromonas caviae.clinical isolates of aeromonas hydrophila, a. sobria, and a. caviae whose fatty acid content had been analyzed by gas-liquid chromatography (glc) displayed the following qualitatively similar glc profiles: 12:0, 14:0, 15:0, 16:0, 17:0, 18:0, 16:1, 18:1, a-15:0, a-17:0, and 3-oh 14:0. the 16:0/17:0 area-percentage ratio separated the clinical aeromonads in accordance with their species designations. aeromonads treated with subminimal inhibitory concentrations of the antibiotic cerulenin displayed ...19883366862
prevalence, species differentiation, and toxigenicity of aeromonas strains in cases of childhood gastroenteritis and in controls.in a 1-year period (january to december 1984), aeromonas strains were isolated from feces of 21 of 561 (3.7%) children with gastroenteritis and from 12 of 576 (2.1%) children without intestinal disturbances (controls). the difference between the two isolation rates was not significant (x2 = 2.2; p greater than 0.05). in five cases of illness other intestinal pathogens were isolated together with aeromonas in the same stool sample. a total of 39 aeromonas strains were detected since in some cases ...19863958147
placental amino acid uptake in normal and complicated pregnancies.amino acids are the essential substrates for fetal growth and catabolism. the fetus is dependent on the placenta for the provision of amino acids, the first step being concentration of amino acids within the syncytiotrophoblast for subsequent transfer to the fetus. a reliable technique for the isolation of human syncytiotrophoblast plasma membrane has been described, and the suitability of this preparation for the study of amino acid uptake and membrane transport has been well documented. using ...19883354595
biological and pathological consequences of feline infectious peritonitis virus infection in the cheetah.an epizootic of feline infectious peritonitis in a captive cheetah population during 1982-1983 served to focus attention on the susceptibility of the cheetah (acinoyx jubatus) to infectious disease. subsequent observations based upon seroepidemiological surveys and electron microscopy of fecal material verified that cheetahs were indeed capable of being infected by coronaviruses, which were antigenically related to coronaviruses affecting domestic cats, i.e. feline infectious peritonitis virus/f ...19882849387
induction of lyme arthritis in lsh hamsters.in studies of experimental lyme disease, a major obstacle has been the unavailability of a suitable animal model. we found that irradiated lsh/ss lak hamsters developed arthritis after injection of borrelia burgdorferi in the hind paws. when nonirradiated hamsters were injected in the hind paws with b. burgdorferi, acute transient synovitis was present. a diffuse neutrophilic infiltrate involved the synovia and periarticular structures. the inflammation was associated with edema, hyperemia, and ...19883410540
clearance of chlamydial elementary bodies from the conjunctival sac.the rate of disappearance of inactivated chlamydia trachomatis elementary body (eb) preparations from the conjunctival sac was studied in monkeys. direct fluorescent antibody (dfa) cytology showed that the majority of eb had been cleared from the eye within 24 hr of the inoculation of 1 x 10(6) inactivated eb, although small numbers of eb could be detected for up to 144 hr. the rate of clearance in normal and ocular immune animals did not differ, and formalin-killed and uv-inactivated ebs disapp ...19873298129
an unusual case of pyogenic meningitis due to aeromonas sobria. 19883382842
[diarrhea caused by aeromonas in ivory coast].the authors drawn epidemiological, clinical conclusions, and the geographical data concerning the diarrheas with aeromonas in ivory-coast. the results relate to a study realized from january 1982 to july 1985. out of the 1,594 excrements 627 germes have been isolated and among which 153 strains of aeromonas if for instance 24.4% of the whole germs. aeromonas caviae is the most frequent species (50%) compared to 27.3% for aeromonas sobria, and 22.7% for aeromonas hydrophila.19863026671
evaluation of prostaglandins as mediators of tubuloglomerular feedback.orthograde and retrograde microperfusion experiments were conducted in sprague-dawley rats to evaluate the participation of vasoconstrictive eicosanoids as mediators of tubuloglomerular feedback (tgf) signals. retrograde perfusion with 160 microm arachidonic acid (aa) added to a hypotonic solution enhanced the stop-flow pressure (sfp) feedback responses compared with those obtained with the control hypotonic solution (delta sfp, 1.6 +/- 0.4 vs. 10.1 +/- 0.7 mmhg with aa). blockade of thromboxane ...19883364575
a procedure of vestibular decompensation for clinical diagnosis. i. animal experiments.vestibular lesions are often so effectively compensated that their diagnosis may be difficult. certain chemicals can decompensate the central vestibular system and thus uncover symptoms present before compensation. this study attempts to devise a procedure which can utilize such decompensation for the benefit of clinical diagnosis. unilateral labyrinthectomies were performed in rats. pathological movements and body positions due to the vestibular loss were compensated within about one week. syst ...19863485723
roles of hormonal and nutritional factors in the regulation of rat liver alcohol dehydrogenase activity and ethanol elimination rate in vivo.fasting reduced the liver alcohol dehydrogenase (adh) activity by 51% (p less than 0.001). insulin, within 2 hr, increased the adh activity found in fasted animals by 28% (p less than 0.02). insulin administration failed to stimulate the reduced adh activity in diabetic rats. however, adh activity in the diabetic-fed rats decreased by 52-54% (p less than 0.001) compared to normal-fed rats regardless of whether they were meal-fed or refed the normal chow. glucagon blocked by 15% (p less than 0.02 ...19883044171
isolation of shiga toxin-resistant vero cells and their use for easy identification of the toxin.shiga toxin-resistant vero cells were isolated by treatment of the cells with nitrosoguanidine. these mutant cells were not affected by shiga toxin at more than 1 microgram/ml, although the parent vero cells were sensitive to 25 pg of the toxin per ml. immunofluorescence studies showed that all the mutant cells had lost toxin-binding capacity. the cytotoxic activities of various bacterial cultures against the parent and mutant cells were compared. all samples from 10 strains of shigella dysenter ...19883045003
differentiation of motile and mesophilic aeromonas strains into species by testing for a camp-like factor.motile and mesophilic aeromonas strains can presumptively be differentiated into species in 18 to 24 h by testing the isolates for the production of a camp-like factor. aeromonas hydrophila strains were positive either aerobically or anaerobically, aeromonas sobria strains were positive only aerobically, and aeromonas caviae strains were always negative.19873611325
species identification of aeromonas strains based on carbon substrate oxidation profiles.twenty clinical strains each of aeromonas hydrophila, aeromonas caviae, and aeromonas sobria were evaluated for their abilities to oxidize one or more of 95 carbon sources on a gn microplate (biolog, hayward, calif.). nine substrates yielded good, discriminatory values for the three species tested. the panel appears to be useful for the species identification of aeromonas isolates originating from human material.19892778077
biochemical and toxigenic characteristics of aeromonas spp. isolated from diseased mammals, moribund and healthy fish.in this study we describe biochemical, toxigenic and surface characteristics of 33 motile aeromonas isolated from diseased mammals, 3 from moribund marine mammals, 24 from healthy fish and 4 from moribund fish. aeromonas hydrophila, a. caviae and a. sobria were isolated from both mammals and fish but at a different incidence. aeromonas hydrophila was the predominant species isolated from clinical specimens; it was isolated from pneumonia, wound infections, septicemia and abortion in horses, catt ...19883188376
measurement of picogram quantities of rat ige from in vitro culture supernatants of b lymphocytes in experimental helminth infections.a double-antibody liquid-phase radioimmunoassay was used to measure picogram quantities of rat ige. the sensitivity of this method (approximately 10 pg/ml) allowed the determination of the production of ige in in vitro culture supernatants of b lymphocytes of infected animals.19863488274
role of enterobius vermicularis in the aetiology of appendicitis.an evaluation was made of the histological material obtained from all 1529 appendices removed during the last 5 years at southmead hospital, bristol, in order to elucidate possible relationships between the incidence of enterobius vermicularis and the origin of inflammation in the appendix. in total, 1419 appendices were removed as cases of clinical appendicitis and 110 in the course of another surgical procedure. e. vermicularis was identified in 2.7 per cent of patients with clinical appendici ...19873651783
partial characterisation of a soluble haemagglutinin from human diarrhoeal isolates of aeromonas.a soluble haemagglutinin has been identified in cell-free culture supernates of human diarrhoeal isolates of aeromonas sobria, a. hydrophila and a. caviae. it was oligomeric; a major peak of haemagglutinating activity had an apparent mol. wt of 780,000 but there was haemagglutinating activity throughout the mol. wt range less than 40,000- greater than 10(6). human group o, a and b, horse, rabbit, chicken and rat erythrocytes, but not those of sheep and cow, were agglutinated by the soluble haema ...19863723584
a practicable variant of the ion exchange method for the radiometric estimation of ornithine decarboxylase activity.a known ornithine decarboxylase assay working with ion exchange separation of [3h]ornithine and [3h]putrescine has been revised. the assay can be performed in disposable 1.5 ml vessels with a total of four pipetting steps. the separation of enzyme substrate and product, respectively, requires 3 h per 50 samples. the detection limit is about 50 pmoles [3h]putrescine formed.19873545892
production of "asao toxin" by aeromonas strains isolated from feces and drinking water.cultures of aeromonas species were tested for production of a toxin recently purified by asao et al. (t. asao, y. kinoshita, s. kozaki, t. uemura, and g. sukaguchi, infect. immun. 46:122-127, 1984) and described as a hemolysin with enterotoxic and cytotoxic activity. the toxin was produced by only 63% of aeromonas sobria strains and by 93% of aeromonas hydrophila strains. also, 54% of a. hydrophila strains produced another cytotoxic entity.19863711306
effect of dilauroylphosphatidylcholine on the acrosome reaction and subsequent penetration of bull spermatozoa into zona-free hamster eggs.incubation of bull sperm with liposomes made with phosphatidylcholine (pc) containing fatty acyl chains of either 10 (pc10) or 12 (pc12) carbons resulted in greater than 90% of the sperm exhibiting an acrosome reaction (ar) within 15 min. liposomes of pc10 rapidly destroyed sperm motility while pc12 acrosome-reacted sperm remained motile for several h. liposomes of pc with greater than or equal to 14-carbon fatty acyl chains had no effect on the ar or motility of sperm. the ar was not induced by ...19863768462
feasibility of same-day identification of members of the family vibrionaceae by the api 20e system.sixty isolates, comprising nine species of the family vibrionaceae, were tested with the api 20e 5-h same-day procedure (analytab products, plainview, n.y.). included were 27 aeromonas hydrophila isolates, 10 aeromonas sobria isolates, 7 aeromonas caviae isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolate. the 5-h profile numbers were specific fo ...19863517061
incidence and virulence of aeromonas species in feces of children with diarrhea.aeromonas spp. occurring in feces of children with diarrhea were studied. forty-eight strains were isolated from 2,025 specimens during a one year period. only 11 of 44 strains tested yielded virulence factors (cytotoxin, hemolysin and hemagglutinin). six strains were identified as aeromonas sobria and five as aeromonas hydrophila. the other strains isolated were identified as aeromonas caviae. the biochemical characteristics associated with virulence factors were a positive voges-proskauer reac ...19863743554
identification of hydroxy fatty acids in aeromonas hydrophila, aeromonas sobria, and aeromonas caviae.gas-liquid chromatographic analysis of fatty acid methyl esters obtained from clinical isolates of aeromonas hydrophila, a. sobria, and a. caviae revealed the presence of two hydroxy fatty acid species, 3-oh 12:0 and 3-oh 14:0.19853934208
morphological changes in rat striatal boutons after chronic methamphetamine and haloperidol treatment.dopaminergic (da) synaptic boutons were identified in rat striatum with an electron microscopic histochemical method. in rats which developed behavioral hypersensitivity after treatment with methamphetamine (map) for about 2 weeks, significantly fewer da boutons were found. this effect was specific to da boutons without mitochondria and was not seen in boutons with mitochondria. the density of granular synaptic vesicles in da boutons, however, did not change significantly. these morphological ch ...19863748472
enterotoxic activity of aeromonas hydrophila and aeromonas sobria. 19836421948
pathogenicity of aeromonas.the ability of 15 aeromonas sobria and 9 aeromonas hydrophila isolates to cause subcutaneous lesions was tested. an inoculum of 10(11) colony forming units/l was injected subcutaneously into mice. surviving animals developed a subcutaneous abscess and/or localised skin sloughing and loss of hair (alopecia). an abscess was induced by all nine a. hydrophila isolates and by three of the 15 a. sobria isolates. the induction of local epidermal sloughing and loss of hair followed challenge with either ...19853981022
comparative in vitro activities of selected antimicrobial agents against aeromonas species and plesiomonas shigelloides.the in vitro antimicrobial susceptibilities of fecal isolates of aeromonas caviae, aeromonas hydrophila, aeromonas sobria, and plesiomonas shigelloides were tested by agar dilution. this in vitro study suggested that ciprofloxacin, enoxacin, norfloxacin, tetracycline, and trimethoprim-sulfamethoxazole might be effective oral agents for treatment of diarrhea involving aeromonas spp. or p. shigelloides.19854004196
in vitro susceptibilities of aeromonas hydrophila, aeromonas sobria, and aeromonas caviae to 22 antimicrobial agents.mics of 22 antimicrobial agents for 60 strains of three aeromonas species were determined by a microdilution method. the newer cephalosporins such as moxalactam, cefotaxime, and cefoperazone, the aminoglycosides, and chloramphenicol, tetracycline, nitrofurantoin, and trimethoprim-sulfamethoxazole inhibited most of the strains studied. within the genus, a. hydrophila was more resistant than either a. caviae or a. sobria to the antibiotics tested.19854037775
demonstration of cholera toxin-related factor in cultures of aeromonas species by enzyme-linked immunosorbent assay.the production of toxins by aeromonas species was examined by the suckling mouse test, the hemolysin test, and the enzyme-linked immunosorbent assay with anticholera enterotoxin. a factor that was immunologically related to cholera enterotoxin was produced by 5 of 14 strains of aeromonas hydrophila and 4 of 15 strains of aeromonas sobria. analysis by these assays and by a test for heat stability suggested that the factor differed from hemolysin and from toxin that was active in the suckling mous ...19854044041
chromosome 16q, eosinophilia, and leukemia. 19853967211
importance of aeromonas sobria in aeromonas bacteremia. 19873805781
aeromonas caviae: an enteric pathogen?of the aeromonas spp. isolated routinely in our laboratory from human feces, about two thirds represent aeromonas caviae. in contrast to aeromonas hydrophila and aeromonas sobria, this species has been considered to be of little enteropathogenic significance due to the absence of known virulence factors. the clinical data presented here suggest a clinical significance of a. caviae, at least in some cases. as yet nothing is known about the pathogenic mechanisms involved.19854066046
[changes of depressor responses to bradykinin with preparations blocking kininase and adrenoreceptors]. 19744154383
virulence markers of mesophilic aeromonads: association of the autoagglutination phenomenon with mouse pathogenicity and the presence of a peripheral cell-associated layer.autoagglutination (aa phenotype) of mesophilic aeromonads in broth was found to be a virulence-associated marker. there were two kinds of aa+ strains: those that spontaneously pelleted (sp+), and those that pelleted only after boiling (pab+). of 79 strains tested, 24 (30%) were aa+, and 18 of these were recovered from clinical specimens. most of the aa+ strains (n = 21) were identified as either aeromonas sobria or aeromonas hydrophila. of the well-documented clinical isolates of a. sobria and a ...19873679544
cephalothin susceptibility as a potential marker for the aeromonas sobria group.fifty-four motile aeromonas strains, composing the three currently recognizable species, were tested for susceptibility to cephalothin by broth dilution and disk agar diffusion assays. cephalothin susceptibility was significantly associated with aeromonas sobria (p less than 0.001) and may be an additional phenotypic marker useful in the identification of this species.19854056009
isolation of mutants defective in alpha-amylase from bacillus subtilis: genetic analyses.the rate of alpha-amylase (ec 3.2.1.1) synthesis in bacillus subtilis is regulated by a gene, amyr, located near a structural gene, amye, for the enzyme. to construct a fine map of the amyr-amye region, we isolated 28 mutants defective in alpha-amylase activity. eleven mutants out of 28 showed no alpha-amylase activity, whereas the other 17 showed less alpha-amylase activity than the parent. out of 17 partially positive alpha-amylase mutants, 10 produced temperature-sensitive enzymes, and 4 prod ...19744212116
comparison of api 20e, api rapid e, and api rapid nft for identification of members of the family vibrionaceae.sixty isolates, from nine species of the family vibrionaceae, were tested by the api 20e, api rapid e, and api rapid nft systems. results were compared with those obtained with standard biochemicals. included were 7 aeromonas caviae isolates, 27 aeromonas hydrophilia isolates, 10 aeromonas sobria isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolat ...19854056003
[determination of lysosomal enzyme activity in the rat liver after ethanol administration]. 19714255377
pediatric gastroenteritis associated with aeromonas sobria and campylobacter jejuni. 19863703054
affinity chromatography of cytosolic nad-linked glycerol-3-phosphate dehydrogenase from normal and neoplastic mammalian tissues. 19744368387
experimental evaluation of the mitroflow pericardial heart valve prosthesis. part ii. pathologic examination.the results of the morphologic and histopathologic examination of 37 bovine pericardial heart valve prostheses that were retrieved after experimental implantation in chacma baboons for periods of one to twelve months are presented in this study. the implanted prostheses consisted of 33 mitroflow valves, size 21 mm, that belonged to four different groups according to the method of preparation of the pericardium (process i to iv) and 4 commercially available ionescu-shiley valves, size 19 mm. all ...19883421507
cell surface changes in virus-induced cell fusion. 2. changes in cell coat thickness. 19714355100
a case report: aeromonas sobria gastroenteritis in an adult.aeromonas sobria, a member of the aeromonas hydrophila group, recently has been recognized as a gastrointestinal pathogen in adults. there have been a few documented cases, and these have come from far eastern countries, namely india, thailand, and indonesia. the authors have isolated a strain of a. sobria from a 62-year-old male patient suffering from diarrheal disease. he had no history of travel outside of the united states for several decades. he presented with a severe diarrheal disease tha ...19853883745
[drug dependence in rats: substitution test and sequential changes in body weight]. 19744477096
uptake and effects of melatonin on the synthesis of proteins by the rat cerebral cortex. 19734766259
different patterns of induction of the two isozymes of alanine aminotransferase of liver of rat as a function of age. 19744826465
giardia intestinalis. a prospective investigation of the prevalence in denmark. 19734697076
[the formation of subjective evaluation of time in man]. 19734772236
cholera-like illness due to aeromonas sobria.a thai woman from bangkok was admitted to a hospital in paris for a cholera-like illness. a culture of her "rice-water" diarrhea was negative for vibrio cholerae and enterotoxigenic escherichia coli but was positive for aeromonas sobria. this strain produced enterotoxin, cytolysin, proteolysin, hemolysin, and a cell-rounding factor. acute -and convalescent-phase sera showed an increase in neutralizing antibodies to enterotoxin, cytolysin, and hemolysin. the enterotoxin, which was labile at 100 c ...19827054327
a toxigenic profile of aeromonas hydrophila and aeromonas sobria isolated from fish.forty strains of motile aeromonas were isolated from healthy and diseased fish. these strains were identified as a. hydrophila or a a. sobria. it was found that only strains of a. hydrophila produced a dermonecrotic factor and two zones of hemolysis on blood agar. all the strains of a. sobria tested and 72% of the a. hydrophila were enterotoxigenic. finally, we observed that only a. hydrophila strains could regularly produce hemolysin at 10 degrees c.19817237279
sequence analysis of two chromosomally mediated inducible beta-lactamases from aeromonas sobria, strain 163a, one a class d penicillinase, the other an ampc cephalosporinase.two beta-lactamase genes from aeromonas sobria, strain 163a, have been cloned and sequenced, one encoding a typical class c cephalosporinase, designated ceps, the other a class d penicillinase, designated amps. ceps is predicted to be a mature protein of 38 kda with a pi value of 7.0. the amino acid sequence of ceps is most similar to that of ampc from pseudomonas aeruginosa (54.7%). amps is predicted to be a mature protein of 27 kda with a pi value of 7.9 that mostly closely resembles blad from ...19958537283
the maximum derivatives of left ventricular pressure and transverse internal diameter as indices of the inotropic state of the left ventricle in conscious dogs.1. in normal, conscious dogs, i.v. infusion of isoprenaline caused inases in heart rate and the maximal derivatives of left ventricular pressure (dp/dt max) and left ventricular internal diameter (dd/dt max). the changes in both derivatives were linearly related to the increase in heart rate.2. increments in heart rate produced by right atrial pacing caused only minimally significant increases in both dp/dt max and dd/dt max at heart rates of 180 beats/min. increases in heart rate, with end dias ...19734772400
studies on bacteriocinogenic lactobacillus isolates from selected nigerian fermented foods.ten bacteriocin-producing (bacteriocinogenic) lactobacillus isolates obtained from three nigerian fermented foods namely: kenkey, ogi and wara were tested against the following indicator organisms: lactobacillus plantarum and food borne pathogens comprising enterotoxigenic escherichia coli, enterohaemorrhagic escherichia coli, serratia, pseudomonas, vibrio cholerae, aeromonas sobria, aeromonas cavice, salmonella typhimurium, plesiomonas shigelloides and staphylococcus aureus. all the bacteriocin ...19958568643
isolation, enumeration, and characterization of aeromonas from polluted waters encountered in diving operations.counts of total viable, aerobic, heterotrophic bacteria, indicator organisms, and aeromonas spp. were made at a diver training site on the anacostia river in washington, d.c. the numbers of aeromonas cells in anacostia river sediment and water increased during periods of elevated water temperature, to maxima of 4 x 10(5) cells per g of sediment and 300 cells per ml of water. correspondingly, aeromonas counts dropped 2 to 4 logs as the water temperature decreased to 0 to 0.5 degrees c. cultures t ...19807396482
variable expression of o-antigen and the role of lipopolysaccharide as an adhesin in aeromonas sobria.on initial isolation of aeromonas sobria 3767 from a diarrhoeal stool specimen, two colony types were obtained: opaque (3767o) and translucent (3767t). strain 3767o consistently produced lipopolysaccharide (lps) core and o-antigen side chain, detectable by sds-page and by western blotting with an o-antigen-specific monoclonal antibody. strain 3767t produced lps core but the amount of o-antigen was dependent on factors including growth medium and bacterial growth phase. strain 3767t exhibited sig ...19947525403
a clinical isolate of aeromonas sobria with three chromosomally mediated inducible beta-lactamases: a cephalosporinase, a penicillinase and a third enzyme, displaying carbapenemase activity.hydrolytic profiles of aeromonas sobria 163a, non-induced and induced, and of a derepressed mutant 163a-m, indicate that this strain has inducible beta-lactamases with activities against cephalosporins, penicillins and carbapenems. three enzymes were identified and two of the beta-lactamase genes, amps, encoding a penicillinase and ceps, encoding a cephalosporinase, were cloned into escherichia coli, permitting analysis of the individual enzymes. isoelectric focusing (ief) analysis using inhibit ...19957759391
association of aeromonas sobria with human infection.fifteen aeromonas isolates from various human infections and nine isolates from polluted water were identified as either aeromonas hydrophila or aeromonas sobria and examined for cytotoxigenicity, enterotoxigenicity, adherence to epithelial cells, and other virulence-associated factors, including proteases, lipases, elastases, and hemolysins. two groups of organisms (i and ii) were distinguishable based on differences in median lethal doses in mice and cytotoxicity for y-1 adrenal cells. group i ...19816112237
distribution of imvc biogroups of aeromonas hydrophila and aeromonas sobria isolated from human, fish and water.aeromonas sobria and a.hydrophila were isolated from infected fish in ratio of 3.5:1 during the outbreak of fish infections from december 1982 to february 1983, while isolates from human diarrheic stool was 1 :2. on the basis of imvc reactions 138 isolates of motile aeromonads could be divided into 11 biogroups, with biogroup 4 showing statistically significant association with infections. nine biogroups of aeromonads which were isolated from infected fish reflected that the outbreak was not cau ...19836658502
serogrouping of motile aeromonas species isolated from healthy and moribund fish.a total of 195 strains of motile aeromonas isolated from fish were characterized as aeromonas hydrophila and aeromonas sobria. in view of the frequency of isolation and the importance of motile aeromonas species as fish pathogens, a serological classificaton of these organisms was attempted. antisera were prepared in rabbits against formalinized whole cell suspensions and against boiled cells of 12 different isolates. seventy-six strains could be grouped by tube agglutination with whole cells as ...19817259164
el fenómeno suicida en cepas de aeromonas mesófilas aisladas de muestras clínicas [the suicide phenomenon in strains of mesophilic aeromonas isolated from clinical specimens].a study was carried out to determine the occurrence of the suicide phenomenon in aeromonas spp strains, isolated from clinical samples, and to establish its relationship with the clinical manifestations of diarrheal diseases. 23 strains were studied: 10, of aeromonas sobria; 7, of aeromonas hydrophila; and 6, of aeromonas caviae. all suicidal strains were isolated from patients with acute diarrheal disease. 3 out of 8, isolated from non-diarrheic feces, showed an intermediate phenotypic profile. ...19937984811
[the identification of aeromonas species isolated in cuba].100 aeromonas strains, isolated from children under 5 years with acute diarrheal diseases from various health centers of the country, were studied from january to july, 1990. using the janda-modified popoff's and veron's model, 63% of the strains were identified in species through primary tests, and 100% of the other 37% were identified in aeromonas sobria, aeromonas hydrophila, and aeromonas caviae, using also supplementary tests.19937984818
ultrastructure and distribution of neuronal and glial elements on the motoneuron surface in the lumbosacral spinal cord of the adult cat. 19694195458
the effect of bacteria infection on mean selected body temperature in the common agama, agama agama: a dose-response study.1. the fever response was studied in 43 common agamas using a self-pairing experiment in which animals received an intraperitoneal injection of sterile saline and an injection of one of six dosages of dead aeromonas sobria (1 x 10(6), 1 x 10(7), 1 x 10(8), 1 x 10(9), 1 x 10(10), and 1 x 10(11) total organisms). 2. the results demonstrated a significant increase in tb (1.6-3.1 degrees c) above the mean selected body temperature (msbt) of the saline injection animals over a bacteria infection rang ...19938101779
prolyl aminopeptidase is not a sulfhydryl enzyme: identification of the active serine residue by site-directed mutagenesis.prolyl aminopeptidase (pap) has been classified as a sulfhydryl enzyme on the basis of its high sensitivity to p-chloromercuribenzoate and heavy metals. recently, however, the possibility of pap being instead a serine enzyme has been raised as a result of two observations--the conservation of some residues among the paps hitherto sequenced, and a similarity to some serine hydrolases. this is the first report describing an attempt to identify the active residue by site-directed mutagenesis. the p ...19947896753
ultrastructure and distribution of neuronal and glial elements on the motoneuron surface in the lumbosacral spinal cord of the adult cat. 19694195458
distribution of mesophilic aeromonas species in raw and ready-to-eat fish and meat products in switzerland.a total of 829 poultry, meat, shellfish and fish products commonly consumed in switzerland were qualitatively and quantitatively examined for the presence of mesophilic aeromonas spp. overall, aeromonads occurred in 24.1% of the samples. raw food products were frequently contaminated (e.g. 94.1% in minced meat), with colony counts up to 6.0 x 10(6)/g. some ready-to-eat products had a relatively high percentage of positive samples as well, such as cooked ham in slices (38.2%), mortadella (12.9%), ...19938268055
aeromonas sobria spontaneous bacterial peritonitis and bacteremia. 19948304329
cloning and characterization of three hemolysin genes from aeromonas salmonicida.two hemolysin genes (ash3 and ash4) of aeromonas salmonicida strain 17-2 and one (ash1) of a. salmonicida atcc14174 were cloned into the cosmid vector charomid 9-36 in escherichia coli dh1. the overall amino acid sequence of the ash3 was similar to that of the aerolysins of aeromonas hydrophila and aeromonas sobria, and hemolysins ahh3, ahh4, and ahh5 of a. hydrophila, and hemolysin asa1 of a. sobria. the sequence of ash4 was similar to that of the ahh1 hemolysin of a. hydrophila. the ash4 hemol ...19938309354
[acute gastroenteritis from aeromonas sobria and pancreatitis: apropos of a case]. 19938332027
inhibitory effect against pathogenic and spoilage bacteria of pseudomonas strains isolated from spoiled and fresh fish.the antibacterial effects of 209 pseudomonas strains isolated from spoiled iced fish and newly caught fish were assessed by screening target organisms in agar diffusion assays. one-third (67 strains) inhibited the growth of one or several of six target organisms (escherichia coli, shewanella putrefaciens, aeromonas sobria, pseudomonas fluorescens, listeria monocytogenes, and staphylococcus aureus), of which s. aureus and a. sobria were the most sensitive. the inhibitory action was most pronounce ...19938357253
wound infection due to aeromonas sobria. 19938110950
characterization of mesophilic aeromonas from clinical specimens by computerized analysis of sds-page protein profiles and by enzymatic activity.mesophilic aeromonas (aeromonas hydrophila, aeromonas sobria, aeromonas caviae) have recently been considered important aetiological agents of human diseases, mainly gastrointestinal infections. although several findings have pointed out the significance of this group of microorganisms as enteric pathogens and suggested the presence of virulence factors, epidemiological and clinical studies are limited by the difficulty of correctly identifying mesophilic aeromonas at the species level. sds-page ...19938264423
enterotoxin production in relation to taxonomic grouping and source of isolation of aeromonas species.a total of 19 of 20 (95%) strains of aeromonas hydrophila biovar hydrophila and 16 of 17 (94%) strains of aeromonas sobria isolated from a variety of clinical and environmental sources were found to be enterotoxin positive. only 2 of 18 (11%) a. hydrophila biovar anaerogenes and 2 of 13 (15%) unidentified aeromonas strains from a similar variety of sources produced enterotoxin. no association was apparent between the source of isolation, in particular diarrheal stools, and enterotoxigenicity; 41 ...19846699147
spectrum of extraintestinal disease due to aeromonas species in tropical queensland, australia.during a 12-month period, the clinical spectrum of extraintestinal disease due to aeromonas species was determined for 56 patients in tropical queensland (australia). forty-six patients acquired their infection in the community, six patients were infected in the hospital, and four patients were colonized. demographic risk factors included male gender (67%) and aboriginal ethnic background (35%). the disease ranged from deep-seated infection (four cases) to soft-tissue infection of varying intens ...19938513068
aeromonas sobria infection with severe muscle degeneration in a patient with alcoholic liver cirrhosis.a 56-yr-old male who had been followed for alcoholic liver disease was admitted for abdominal pain and a high fever. gastrointestinal endoscopy revealed bleeding esophageal varices that were treated by endoscopic sclerotherapy. blood culture on admission was positive for aeromonas sobria. then skin bullas and ulcers and severe muscle degeneration developed. the patient died despite extensive treatment with antibiotics. a. sobria infection in patients with liver cirrhosis is rare.19958540527
growth and exotoxin production at low temperatures by aeromonas strains in meat slurries.the ability of enterotoxigenic strains of aeromonas hydrophila and aeromonas sobria to produce exotoxins (haemolysin and enterotoxin) and exoenzymes (protease and lipase), as well as their growth in meat slurries stored at low temperatures (5 and 12 degrees c) was investigated. the results showed that aeromonas grew well in meat slurries stored at low temperatures in the presence of background flora. exotoxins and exoenzymes were not detected in filtrates from meat slurries inoculated with enter ...19968643030
[rat-flea index in plague control campaigns]. 19674235703
aeromonas cytotonic enterotoxin cross reactive with cholera toxin.isolation by affinity chromatography from crude culture filtrate of aeromonas sobria of protein that cross reacted with cholera toxin (ct) revealed a toxin that produced fluid accumulation in rat ileal loops and in infant mice and caused rounding of y1 adrenal cells. all these activities were neutralised by antiserum to ct. there was no haemolytic or cytotoxic activity associated with this ct-cross reactive cytotonic enterotoxin. ct-cross reactive material detected in enzyme linked immunosorbent ...19873560188
determination of antimony levels in organs of mice, treated with an antimony-albumin complex, by atomic absorption spectroscopy. 19873609261
endotoxin in aeromonas sobria infection: speculative pathogenesis and treatment strategies in advanced liver disease with soft-tissue infection. 19968678032
[bacteriological studies of travellar's diarrhoea. 5) analysis of enteropathogenic bacteria at osaka airport quarantine station from january 1992 through september 3rd, 1994].during the last 2 years and 8 months before the closure of osaka airport quarantine station (from jan. 1992 to sep. 3, 1994), a total of 7,421,909 overseas travellers were quarantined. 15,919 reported themselves of suffering from diarrhoea. bacteriological examination of a total of 6,031 individuals' stools were performed. 1) various enteropathogenic bacteria were isolated from 31.2% of the stools examined. isolated species were as follows: plesiomonas shigelloides, 1,127 cases (59.9%); vibrio p ...19968822051
Displaying items 101 - 200 of 544