Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
[rat-flea index in plague control campaigns]. 19674235703
ultrastructure and distribution of neuronal and glial elements on the motoneuron surface in the lumbosacral spinal cord of the adult cat. 19694195458
ultrastructure and distribution of neuronal and glial elements on the motoneuron surface in the lumbosacral spinal cord of the adult cat. 19694195458
[determination of lysosomal enzyme activity in the rat liver after ethanol administration]. 19714255377
cell surface changes in virus-induced cell fusion. 2. changes in cell coat thickness. 19714355100
giardia intestinalis. a prospective investigation of the prevalence in denmark. 19734697076
uptake and effects of melatonin on the synthesis of proteins by the rat cerebral cortex. 19734766259
[the formation of subjective evaluation of time in man]. 19734772236
the maximum derivatives of left ventricular pressure and transverse internal diameter as indices of the inotropic state of the left ventricle in conscious dogs.1. in normal, conscious dogs, i.v. infusion of isoprenaline caused inases in heart rate and the maximal derivatives of left ventricular pressure (dp/dt max) and left ventricular internal diameter (dd/dt max). the changes in both derivatives were linearly related to the increase in heart rate.2. increments in heart rate produced by right atrial pacing caused only minimally significant increases in both dp/dt max and dd/dt max at heart rates of 180 beats/min. increases in heart rate, with end dias ...19734772400
different patterns of induction of the two isozymes of alanine aminotransferase of liver of rat as a function of age. 19744826465
affinity chromatography of cytosolic nad-linked glycerol-3-phosphate dehydrogenase from normal and neoplastic mammalian tissues. 19744368387
[drug dependence in rats: substitution test and sequential changes in body weight]. 19744477096
[changes of depressor responses to bradykinin with preparations blocking kininase and adrenoreceptors]. 19744154383
isolation of mutants defective in alpha-amylase from bacillus subtilis: genetic analyses.the rate of alpha-amylase (ec 3.2.1.1) synthesis in bacillus subtilis is regulated by a gene, amyr, located near a structural gene, amye, for the enzyme. to construct a fine map of the amyr-amye region, we isolated 28 mutants defective in alpha-amylase activity. eleven mutants out of 28 showed no alpha-amylase activity, whereas the other 17 showed less alpha-amylase activity than the parent. out of 17 partially positive alpha-amylase mutants, 10 produced temperature-sensitive enzymes, and 4 prod ...19744212116
characterization of 5.8s ribosomal ribonucleic acid in neurospora crassa.neurospora crassa ribosomes contain a species of ribonucleic acid (rna) of molecular weight 54,000, similar to 5.8s ribosomal rna previously described for other eukaryotic organisms. the 5.8s rna from n. crassa was found to be released by heat treatment at 60 c from 25s ribosomal rna but not from 18s ribosomal rna. the base composition of n. crassa 5.8s rna was similar to that of 5.8s rna from saccharomyces cerevisiae, but differed from animal 5.8s rna. during the course of this study, it was di ...1975126993
k+, mg2+-aspartate (kmg-asp)--mediated prevention of isoproterenol(iso)-induced metabolic changes in the myocardium.isoproterenol (iso) in a single dose of 7.5 mg/kg b.w. i.v was found to produce alterations of cardiac metabolism in dogs. after 2 h, high energy phosphate stores and glycogen were reduced, whereas the levels of lactate, pyruvate, the lactate/pyruvate ratio and myofibrillar atpase activity were elevated. ca2+ -accumulation by sarcoplasmic reticulum (sr) and mitochondria were increased (p less than 0.01). after 24 h, a partial recovery in the parameters followed could be observed. only myofibrill ...1975126687
[separation and characterization of subcellular organoids by tissue fractionation]. 1975152443
[pathological studies on corynebacterial ulcerative entero-colitis in rats treated with acth (author's transl)].ulcerative entero-colitis was developed in 6-week-old male sprague-dawley rats treated daily with acth (4 mg/kg. s. c.) as well as necrotic purulent lesions in liver, kidney, lung or heart. incidence of ulcerative lesions was 6.3% in farm-a rats and 56% in farm-b rats. although ulcerative lesions were mostly observed in cecum, the similar lesions were also detected in distal ileum or proximal colon in some cases. histologically, the lesions were characterized by focal necrosis demarcated from su ...1975174920
[salmonellosis carrier state]. 19751214656
adaptation of cells derived from human malignant tumours to growth in vitro.forty-five human malignant tumour specimens were cultivated in vitro in an attempt to determine the necessary conditions for tumour cell maintenance and to establish permanently-growing cell lines. continuously-growing cultures were derived from five tumours, including carcinomas of the oesophagus and colon, a hepatoma, a mesothelioma and a retroperitoneal sarcoma. the carcinoma of the oesophagus and the hepatoma, which have adapted fully to in vitro conditions, can be regarded as established ce ...1976184551
the accumulation of cyclic amp and cyclic gmp in guinea pig brain slices. effect of calcium ions, norepinephrine and adenosine. 1977195635
thyroid - hormone effects on steroid - hormone metabolism. 1977266410
the laboratory surveillance of influenza epidemics in the united kingdom 1968-1976.the extensive laboratory investigations of respiratory disease in the u.k. over many years have demonstrated the frequency with which influenza viruses, both a and b, are found each winter. only rarely are none isolated. these findings correlate well with other indicators of influenza such as increases in sickness benefit claims and in deaths attributed to influenza and pneumonia. however, outside these demonstrable peaks of incidence influenza viruses have been found to circulate over considera ...1977267671
[fine structure of oral spirochetes, especially axial fibrils and cyst like structure (author's transl)]. 1977268542
[mycoplasma infections]. 1977269619
isolation of enterotoxigenic aeromonas from fish.this paper reports two different enterotoxigenic classes of the genus aeromonas isolated from fishes. strains of a. sobria were isolated from healthy fishes only wheras strains of a. hydrophila were isolated from both healthy and moribund fishes. aeromonas sobria and hydrophilia strains produced a cytotoxic factor and were highly proteolytic. seventy-five percent (75%) of the a. sobria strains produced only one type of hemolysis on 5% blood agar, but 92% of the a. hydrophilia produced two types ...197771190
pseudomembranous colitis. 197877373
cryopreservation of antibody-coated human erythrocytes. 1978105832
[kinetics of the serological response in the experimental primary hydatid disease of mice infected with echinococcus granulosus embryophores (author's transl)].a serological response kinetics study was carried out in cf-1 mice orally infected with echinococcus granulosus embryophores using immuno-electrophoresis, double diffusion, latex agglutination and passive hemagglutination. starting from the 76th day after the infection, serum antibodies reach the minimum detectable level by immunoelectrophoresis test. band number 5, now considered as specific for e. granulosus, was proved to be the first precipitating system exhibited in this test. double diffus ...1978105664
[evolution and transformation in animal organism of some polychlorobiphenyls (author's transl)].intoxication by pure polychlorobiphenyls and a commercial product is studied, in rats, after intraperitoneal and oral administration. their fixation and excretion of their metabolites is greater by intraperitoneal than by oral administration. the only hydroxy metabolites, identified by means of synthesis, gas chromatography and mass spectrometry are, all, derivatives from trichlorobiphenyl. it appears that administration of commercial product induces a more rapid degradation of these compounds i ...1978105430
clinical studies on pipemidic acid. 1978101691
serologic typing of rickettsiae of the spotted fever group by microimmunofluorescence. 1978101593
long term effects of myochrysine in articular cartilage.intra-articularly administered sodium aurothiomalate (myochrysine) produced aurosomes containing characteristic electron dense contents (indicating the presence of gold), in the chondrocytes of rabbit articular cartilage. at first the aurosomes were bounded by a membrane but later the electron dense contents were seen lying free in the cytoplasmic matrix. such deposits were detectable up to 14 months after injection of myochrysine but none were found at later time intervals (18 months and 2 year ...197897856
current concepts in ophthalmology. ocular infections. 197896339
antiviral agents: action and clinical use.the development of antiviral agents has been hindered by a variety of problems. there are fundamental biological differences between viruses and other infectious agents. viruses are strictly dependent on cellular metabolic processes and possess very limited intrinsic enzyme systems and building blocks which may serve as targets for drugs. antiviral drugs must also possess the ability to enter the host cell. viral replication consists of a series of events, each of which can be interfered with, l ...197992398
[a comparison of the immunosuppressive effect of normal and purified alpha-globulin fraction of human plasma]. 197992373
the role of eosinophil receptors in the non-genomic response to oestrogens in the uterus. 197990751
antigenic determinants in the post-mitochondrial lung fraction. 197990649
characterization of in vitro proliferative responses of human lymphocytes to leishmanial antigens.leishmaniasis is an intracellular protozoal infection for which host defense is believed to depend on cellular immune mechanisms. the in vitro proliferative responses of lymphocytes from patients with leishmaniasis and from control subjects to leishmanial antigens were examined. only lymphocytes from patients responded to 1 microgram of leishmanial antigen/ml, whereas both patient and control lymphocytes responded to 10 micrograms/ml. the nonspecific responses were most likely not due to a mitog ...197990105
the association of carbohydrate with tubulin and in vitro assembled microtubules from bovine brain.phosphocellulose-purified tubulin (pc tubulin) was analyzed for neutral and amino sugar content, which was found to be 8.3 +/- 0.11 and 0.8 +/- 0.02 mol/mol dimer, respectively. a histochemical-electron-microscopic investigation was undertaken to attempt to localize carbohydrate associated with polymerized microtubules (mt). outer diameters of mt assembled in vitro from bovine brain mt protein (tubulin and microtubule associated proteins) were found to increase upon treatment with ruthenium red, ...197990041
modulation of cellular interactions between c3h/10t1/2 cells and their transformed counterparts by phosphodiesterase inhibitors.it has been demonstrated previously that nontransformed c3h/10t1/2cl8 mouse embryo fibroblasts (10t1/2) can induce a state of reversible growth inhibition in cocultured malignantly transformed mouse fibroblasts and that this inhibition is modulated by serum concentration. the present study suggests that cyclic nucleotides may be implicated in this intercellular communication. the phosphodiesterase inhibitors theophylline, caffeine, and 3-isobutyl-1-methylxanthine (ibx) at concentrations of 10(-3 ...197989899
control of v kappa expression in the mouse. i. unexpected expression of the v kappa allele, igk-pcb, in a somatic cell hybrid of akr (igk-pca) origin. 197989158
the role of p23,30-bearing human macrophages in antigen-induced t lymphocyte responses. 197988270
in vitro synthesis of infectious transforming dna by the avian sarcoma virus reverse transcriptase.infectious dna molecules, capable of transforming chicken embryo fibroblasts, can be synthesized by the rous sarcoma virus-associated reverse transcriptase in vitro. the optimal enzymatic conditions employed for infectious dna synthesis also facilitate maximum synthesis of genome length dna. analysis of the dna product synthesized by detergent-disrupted rous sarcoma virus under these conditions indicates that dna complementary to viral rna (minus-strand dna) is genome length in size, whereas dna ...197985719
transcription and translation in a pleiotropic streptomycin-resistant mutant of escherichia coli.the role of the ribosomal protein s12 (streptomycin protein) in ribosome function and in other metabolic processes in the cell has been investigated. a spontaneous streptomycin-resistant strain of escherichia coli (sm3) carrying a mutation in the rpsl gene is deficient in its ability to induce the synthesis of the enzyme bets-galactosidase. it was demonstrated that the reduced rate of enzyme synthesis results from deficiencies in both the transcription of the lactose operon and translation of th ...1979104958
[isolation of treponemas from the colon of pigs with clinical dysentery].optimal culture conditions in artificial nutritive media were determined for a defined avirulent strain of treponema hyodysenteriae and for four field strains of treponemas in pigs with clinical dysentery. the treponemas were isolated with the use of milliporous filters with pores of 0.3 micrometer in diameter, which were located on the surface of blood agar. no significant difference in the influence of equine, bovine or sheep blood on the growth of treponemas was determined. the commercial amo ...1979105448
[fundamental studies and clinical evaluation of pc-904 in urinary tract infection (author's transl)].measurement of mics of pc-904, cbpc and gm against 135 strains of pseudomonas aeruginosa isolated from patients with urinary tract infections was performed. susceptibility to pc-904 of pseudomonas aeruginosa was found to be 3 approximately 7 times more sensitive than that to cbpc and as same as that to gm with inoculum size of 10(8)/ml and 10(6)/ml. serum level of pc-904 reached a peak of 38.5 microgram/ml 15 minutes after 0.5 g intravenous administration. urinary recovery rate unit 6 hours afte ...1979108428
microscopic changes in rat tongue following experimental cryosurgery.histological changes were studied in the rat tongue for varying periods of time up to 14 days following the application of a cryoprobe to the dorsal surface of rat tongue. within minutes of the application, focal subepithelial vesiculation and hyperemia occurred. at 3 h there was evidence of damage to capillary walls, resulting in hemorrhage. arterioles and venules were thrombosed and there were degenerative changes in the muscle layer of their walls. at 6 h ventral epithelium showed evidence of ...1979108379
[bilirubin in the blood serum of clinically healthy cows and those ill with ketosis and hepatopathies].studied was the bilirubin equilibrium in 62 normal cows, 52 cows with clinical ketosis, 31 cows with diffuse liver injury, and 14 cows that went off food for five days. a moderate rise of the total bilirubin of mixed type was found in ketosis-affected cows. statistically significant proved the changes in the free fraction, however, there were no persuasive data that they were due to superproduction of bilirubin. changes in the total bilirubin similar to those in cows with ketosis were establishe ...1979516442
isolation, enumeration, and characterization of aeromonas from polluted waters encountered in diving operations.counts of total viable, aerobic, heterotrophic bacteria, indicator organisms, and aeromonas spp. were made at a diver training site on the anacostia river in washington, d.c. the numbers of aeromonas cells in anacostia river sediment and water increased during periods of elevated water temperature, to maxima of 4 x 10(5) cells per g of sediment and 300 cells per ml of water. correspondingly, aeromonas counts dropped 2 to 4 logs as the water temperature decreased to 0 to 0.5 degrees c. cultures t ...19807396482
a toxigenic profile of aeromonas hydrophila and aeromonas sobria isolated from fish.forty strains of motile aeromonas were isolated from healthy and diseased fish. these strains were identified as a. hydrophila or a a. sobria. it was found that only strains of a. hydrophila produced a dermonecrotic factor and two zones of hemolysis on blood agar. all the strains of a. sobria tested and 72% of the a. hydrophila were enterotoxigenic. finally, we observed that only a. hydrophila strains could regularly produce hemolysin at 10 degrees c.19817237279
serogrouping of motile aeromonas species isolated from healthy and moribund fish.a total of 195 strains of motile aeromonas isolated from fish were characterized as aeromonas hydrophila and aeromonas sobria. in view of the frequency of isolation and the importance of motile aeromonas species as fish pathogens, a serological classificaton of these organisms was attempted. antisera were prepared in rabbits against formalinized whole cell suspensions and against boiled cells of 12 different isolates. seventy-six strains could be grouped by tube agglutination with whole cells as ...19817259164
association of aeromonas sobria with human infection.fifteen aeromonas isolates from various human infections and nine isolates from polluted water were identified as either aeromonas hydrophila or aeromonas sobria and examined for cytotoxigenicity, enterotoxigenicity, adherence to epithelial cells, and other virulence-associated factors, including proteases, lipases, elastases, and hemolysins. two groups of organisms (i and ii) were distinguishable based on differences in median lethal doses in mice and cytotoxicity for y-1 adrenal cells. group i ...19816112237
diabetes mellitus and periodontal disease. 1981273875
aeromonas sobria in chlorinated drinking water supplies.aeromonas species were recovered from over 27% of 183 chlorinated drinking water samples collected during an 18-month period. sixteen of 20 isolates tested elicited a cytotoxic response by y-1 mouse adrenal cells. none of the strains was either enterotoxigenic by the rabbit ligated ileal loop assay, exhibited piliation, or showed significant mannose resistant adherence to human buccal cells. theaeromonas isolates were further identified to bea. sobria and were resistant to ampicillin and suscept ...198224226050
cholera-like illness due to aeromonas sobria.a thai woman from bangkok was admitted to a hospital in paris for a cholera-like illness. a culture of her "rice-water" diarrhea was negative for vibrio cholerae and enterotoxigenic escherichia coli but was positive for aeromonas sobria. this strain produced enterotoxin, cytolysin, proteolysin, hemolysin, and a cell-rounding factor. acute -and convalescent-phase sera showed an increase in neutralizing antibodies to enterotoxin, cytolysin, and hemolysin. the enterotoxin, which was labile at 100 c ...19827054327
enterotoxic activity of aeromonas hydrophila and aeromonas sobria. 19836421948
distribution of imvc biogroups of aeromonas hydrophila and aeromonas sobria isolated from human, fish and water.aeromonas sobria and a.hydrophila were isolated from infected fish in ratio of 3.5:1 during the outbreak of fish infections from december 1982 to february 1983, while isolates from human diarrheic stool was 1 :2. on the basis of imvc reactions 138 isolates of motile aeromonads could be divided into 11 biogroups, with biogroup 4 showing statistically significant association with infections. nine biogroups of aeromonads which were isolated from infected fish reflected that the outbreak was not cau ...19836658502
enterotoxin production in relation to taxonomic grouping and source of isolation of aeromonas species.a total of 19 of 20 (95%) strains of aeromonas hydrophila biovar hydrophila and 16 of 17 (94%) strains of aeromonas sobria isolated from a variety of clinical and environmental sources were found to be enterotoxin positive. only 2 of 18 (11%) a. hydrophila biovar anaerogenes and 2 of 13 (15%) unidentified aeromonas strains from a similar variety of sources produced enterotoxin. no association was apparent between the source of isolation, in particular diarrheal stools, and enterotoxigenicity; 41 ...19846699147
biotyping of aeromonas isolates as a correlate to delineating a species-associated disease spectrum.a group of 147 aeromonas isolates from diverse clinical and environmental sources was subjected to the biotyping scheme of popoff and veron. of the 147 isolates biotyped, 137 (93%) could be identified, with aeromonas hydrophila predominating (48%) and equal percentages (25 to 27%) of the other two species (aeromonas sobria and aeromonas caviae). a number of additional biochemical properties were found to be significantly associated with one or more of these three species. these included lysine d ...19846690467
the value of a simple medical questionnaire in dentistry.a short specific medical questionnaire was given to 1125 new patients attending for their initial examination at the perth dental hospital. a positive history was given by 398 patients. a wide variety of conditions was revealed and the dental significance is discussed. the extent of the information provided illustrates that the simple questionnaire is valuable in exposing conditions which may affect dental treatment.1984270975
morphological correlates of synaptic transmission in lamprey spinal cord.the dye procion brown was used to identify in the light and electron microscope, synaptic contacts made between monosynaptically coupled neurons in the lamprey spinal cord whose synaptic interaction had been recorded. synaptic contacts were made on different dendrites of the postsynaptic cell at different distances from the soma. some of the contacts were made on dentritic spines and some on the smooth shaft of the dentrites. serial sections through synaptic contacts made on dendritic processess ...1985176330
secondary hypertriglyceridemia and hyperlipoproteinemia in patients with primary asymptomatic gout.we carefully selected 30 men with primary gout, rendered asymptomatic by therapy, to examine the frequency and type of hyperlipidemia and hyperlipoproteinemia, with the objective of determining whether serum uric acid, alcohol intake, liver function, kidney function, and (or) drugs were participating in the secondary lipid disorder. sixty-one age- and sex-matched men were used as controls. about 73% of the gout patients had hypertriglyceridemia, 1.6-fold the frequency found in the control group. ...1985262176
the ada conference on increasing access to dental care. a report. 1985268525
comparative in vitro activities of selected antimicrobial agents against aeromonas species and plesiomonas shigelloides.the in vitro antimicrobial susceptibilities of fecal isolates of aeromonas caviae, aeromonas hydrophila, aeromonas sobria, and plesiomonas shigelloides were tested by agar dilution. this in vitro study suggested that ciprofloxacin, enoxacin, norfloxacin, tetracycline, and trimethoprim-sulfamethoxazole might be effective oral agents for treatment of diarrhea involving aeromonas spp. or p. shigelloides.19854004196
demonstration of cholera toxin-related factor in cultures of aeromonas species by enzyme-linked immunosorbent assay.the production of toxins by aeromonas species was examined by the suckling mouse test, the hemolysin test, and the enzyme-linked immunosorbent assay with anticholera enterotoxin. a factor that was immunologically related to cholera enterotoxin was produced by 5 of 14 strains of aeromonas hydrophila and 4 of 15 strains of aeromonas sobria. analysis by these assays and by a test for heat stability suggested that the factor differed from hemolysin and from toxin that was active in the suckling mous ...19854044041
in vitro susceptibilities of aeromonas hydrophila, aeromonas sobria, and aeromonas caviae to 22 antimicrobial agents.mics of 22 antimicrobial agents for 60 strains of three aeromonas species were determined by a microdilution method. the newer cephalosporins such as moxalactam, cefotaxime, and cefoperazone, the aminoglycosides, and chloramphenicol, tetracycline, nitrofurantoin, and trimethoprim-sulfamethoxazole inhibited most of the strains studied. within the genus, a. hydrophila was more resistant than either a. caviae or a. sobria to the antibiotics tested.19854037775
chromosome 16q, eosinophilia, and leukemia. 19853967211
pathogenicity of aeromonas.the ability of 15 aeromonas sobria and 9 aeromonas hydrophila isolates to cause subcutaneous lesions was tested. an inoculum of 10(11) colony forming units/l was injected subcutaneously into mice. surviving animals developed a subcutaneous abscess and/or localised skin sloughing and loss of hair (alopecia). an abscess was induced by all nine a. hydrophila isolates and by three of the 15 a. sobria isolates. the induction of local epidermal sloughing and loss of hair followed challenge with either ...19853981022
a case report: aeromonas sobria gastroenteritis in an adult.aeromonas sobria, a member of the aeromonas hydrophila group, recently has been recognized as a gastrointestinal pathogen in adults. there have been a few documented cases, and these have come from far eastern countries, namely india, thailand, and indonesia. the authors have isolated a strain of a. sobria from a 62-year-old male patient suffering from diarrheal disease. he had no history of travel outside of the united states for several decades. he presented with a severe diarrheal disease tha ...19853883745
identification of hydroxy fatty acids in aeromonas hydrophila, aeromonas sobria, and aeromonas caviae.gas-liquid chromatographic analysis of fatty acid methyl esters obtained from clinical isolates of aeromonas hydrophila, a. sobria, and a. caviae revealed the presence of two hydroxy fatty acid species, 3-oh 12:0 and 3-oh 14:0.19853934208
comparison of api 20e, api rapid e, and api rapid nft for identification of members of the family vibrionaceae.sixty isolates, from nine species of the family vibrionaceae, were tested by the api 20e, api rapid e, and api rapid nft systems. results were compared with those obtained with standard biochemicals. included were 7 aeromonas caviae isolates, 27 aeromonas hydrophilia isolates, 10 aeromonas sobria isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolat ...19854056003
cephalothin susceptibility as a potential marker for the aeromonas sobria group.fifty-four motile aeromonas strains, composing the three currently recognizable species, were tested for susceptibility to cephalothin by broth dilution and disk agar diffusion assays. cephalothin susceptibility was significantly associated with aeromonas sobria (p less than 0.001) and may be an additional phenotypic marker useful in the identification of this species.19854056009
aeromonas caviae: an enteric pathogen?of the aeromonas spp. isolated routinely in our laboratory from human feces, about two thirds represent aeromonas caviae. in contrast to aeromonas hydrophila and aeromonas sobria, this species has been considered to be of little enteropathogenic significance due to the absence of known virulence factors. the clinical data presented here suggest a clinical significance of a. caviae, at least in some cases. as yet nothing is known about the pathogenic mechanisms involved.19854066046
[aquatic contamination of wounds by aeromonas sobria after bathing in the river]. 19853160041
hypotensive effects after topical and intra-arterial administration of alpha-adrenergic agonists in isolated perfused rabbit eyes.the influence of nonselective and selective alpha-adrenergic agonists on intraocular pressure (iop) was studied in isolated arterially perfused rabbit eyes. drugs were given intra-arterially or topically. with either route of administration, no initial hypertensive phase was observed after l-epinephrine, l-norepinephrine, l-phenylephrine (alpha 1), or methoxamine (alpha 1), but there was an immediate ocular hypotensive effect. the alpha 2-agonist b-ht 920 caused a nonsignificant 1 mm hg fall in ...19852866480
identification and characterization of mg2+-dependent phosphotyrosyl protein phosphatase from rat liver cytosol.although highly purified preparations of mg2+-dependent phosphoseryl protein phosphatase (also designated phosphatase ia or phosphatase 2c) dephosphorylated phosphotyrosyl histone, the activity has been resolved from phosphatase ia by polyacrylamide gel electrophoresis at ph 9.5. this novel phosphotyrosyl-specific protein phosphatase absolutely requires mg2+ or mn2+ for activity, is inhibited by zn2+, vanadate and fluoride, and has an optimal ph of 9.0 and mr = 50,000. certain properties of this ...19863022716
whole-body autoradiography of [3h]dihydrostreptomycin in guinea pigs and rats: the labelling of the inner ear in relation to other tissues.[3h]dihydrostreptomycin was given intramuscularly to young pigmented guinea pigs and rats. whole-body autoradiography, combined with densitometric measurement of the blackening of the autoradiograms, and liquid scintillation counting were used to determine the levels of radioactivity in the inner ear in relation to blood and other tissues. it was found that there was an accumulation of radioactivity in the perilymph of both the cochlear and vestibular parts of the labyrinth. the labelling of the ...19862422870
fatal infection with aeromonas sobria and plesiomonas shigelloides. 19863081156
prevalence, species differentiation, and toxigenicity of aeromonas strains in cases of childhood gastroenteritis and in controls.in a 1-year period (january to december 1984), aeromonas strains were isolated from feces of 21 of 561 (3.7%) children with gastroenteritis and from 12 of 576 (2.1%) children without intestinal disturbances (controls). the difference between the two isolation rates was not significant (x2 = 2.2; p greater than 0.05). in five cases of illness other intestinal pathogens were isolated together with aeromonas in the same stool sample. a total of 39 aeromonas strains were detected since in some cases ...19863958147
[diarrhea caused by aeromonas in ivory coast].the authors drawn epidemiological, clinical conclusions, and the geographical data concerning the diarrheas with aeromonas in ivory-coast. the results relate to a study realized from january 1982 to july 1985. out of the 1,594 excrements 627 germes have been isolated and among which 153 strains of aeromonas if for instance 24.4% of the whole germs. aeromonas caviae is the most frequent species (50%) compared to 27.3% for aeromonas sobria, and 22.7% for aeromonas hydrophila.19863026671
a procedure of vestibular decompensation for clinical diagnosis. i. animal experiments.vestibular lesions are often so effectively compensated that their diagnosis may be difficult. certain chemicals can decompensate the central vestibular system and thus uncover symptoms present before compensation. this study attempts to devise a procedure which can utilize such decompensation for the benefit of clinical diagnosis. unilateral labyrinthectomies were performed in rats. pathological movements and body positions due to the vestibular loss were compensated within about one week. syst ...19863485723
measurement of picogram quantities of rat ige from in vitro culture supernatants of b lymphocytes in experimental helminth infections.a double-antibody liquid-phase radioimmunoassay was used to measure picogram quantities of rat ige. the sensitivity of this method (approximately 10 pg/ml) allowed the determination of the production of ige in in vitro culture supernatants of b lymphocytes of infected animals.19863488274
pediatric gastroenteritis associated with aeromonas sobria and campylobacter jejuni. 19863703054
production of "asao toxin" by aeromonas strains isolated from feces and drinking water.cultures of aeromonas species were tested for production of a toxin recently purified by asao et al. (t. asao, y. kinoshita, s. kozaki, t. uemura, and g. sukaguchi, infect. immun. 46:122-127, 1984) and described as a hemolysin with enterotoxic and cytotoxic activity. the toxin was produced by only 63% of aeromonas sobria strains and by 93% of aeromonas hydrophila strains. also, 54% of a. hydrophila strains produced another cytotoxic entity.19863711306
partial characterisation of a soluble haemagglutinin from human diarrhoeal isolates of aeromonas.a soluble haemagglutinin has been identified in cell-free culture supernates of human diarrhoeal isolates of aeromonas sobria, a. hydrophila and a. caviae. it was oligomeric; a major peak of haemagglutinating activity had an apparent mol. wt of 780,000 but there was haemagglutinating activity throughout the mol. wt range less than 40,000- greater than 10(6). human group o, a and b, horse, rabbit, chicken and rat erythrocytes, but not those of sheep and cow, were agglutinated by the soluble haema ...19863723584
incidence and virulence of aeromonas species in feces of children with diarrhea.aeromonas spp. occurring in feces of children with diarrhea were studied. forty-eight strains were isolated from 2,025 specimens during a one year period. only 11 of 44 strains tested yielded virulence factors (cytotoxin, hemolysin and hemagglutinin). six strains were identified as aeromonas sobria and five as aeromonas hydrophila. the other strains isolated were identified as aeromonas caviae. the biochemical characteristics associated with virulence factors were a positive voges-proskauer reac ...19863743554
morphological changes in rat striatal boutons after chronic methamphetamine and haloperidol treatment.dopaminergic (da) synaptic boutons were identified in rat striatum with an electron microscopic histochemical method. in rats which developed behavioral hypersensitivity after treatment with methamphetamine (map) for about 2 weeks, significantly fewer da boutons were found. this effect was specific to da boutons without mitochondria and was not seen in boutons with mitochondria. the density of granular synaptic vesicles in da boutons, however, did not change significantly. these morphological ch ...19863748472
effect of dilauroylphosphatidylcholine on the acrosome reaction and subsequent penetration of bull spermatozoa into zona-free hamster eggs.incubation of bull sperm with liposomes made with phosphatidylcholine (pc) containing fatty acyl chains of either 10 (pc10) or 12 (pc12) carbons resulted in greater than 90% of the sperm exhibiting an acrosome reaction (ar) within 15 min. liposomes of pc10 rapidly destroyed sperm motility while pc12 acrosome-reacted sperm remained motile for several h. liposomes of pc with greater than or equal to 14-carbon fatty acyl chains had no effect on the ar or motility of sperm. the ar was not induced by ...19863768462
feasibility of same-day identification of members of the family vibrionaceae by the api 20e system.sixty isolates, comprising nine species of the family vibrionaceae, were tested with the api 20e 5-h same-day procedure (analytab products, plainview, n.y.). included were 27 aeromonas hydrophila isolates, 10 aeromonas sobria isolates, 7 aeromonas caviae isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolate. the 5-h profile numbers were specific fo ...19863517061
different effects of the ionophore a-23187 and d-glucose on 45ca2+ fluxes in isolated islets of ob/ob-mice.fluxes of 45ca2+ were studied in beta-cell rich islets of non-inbred ob/ob-mice, using lacl3 to wash out extra-cellular and superficially bound 45ca2+. the ionophore a-23187 (10 microm) increased the 45ca2+ uptake in islets both at 3 and 20 mm d-glucose, the effect being more pronounced after 10 min than after 120 min of incubation. in incubations for 120 min, 20 mm d-glucose induced a higher uptake of 45ca2+ than did a-23187. the ionophore enhanced the unidirectional efflux of 45ca2+ from prelo ...1986362823
scintigraphic study of the gastrointestinal transit of a liquid meal in patients with chronic chagas' disease.gastric emptying and small bowel transit of a liquid meal (isotonic dextrose) were assessed by a scintigraphic technique in 16 patients with chagas' disease involving the esophagus and/or the colon, including one case with mega-jejunum, and in 10 control subjects. initial gastric emptying was faster in the chagas' disease group than in controls (gastric retention at 15 min, median and (range): 52% (15-86) vs 71% (43-97), p < 0.01) although there was no significant difference in total gastric emp ...19861339510
[specialization in periodontology].in addition to the demand that periodontal therapy should be the task of any stomatologist, the considerable morbidity and the aetiopathological special features of periodontal diseases call for specialists. periodontal basic care and specialized therapy easy to delimitate. the specified stomatological curriculum allows for the demands on periodontology; it is imperative to enforce them in education and continuing education.1986268701
the immediate overlay denture. 1987269213
importance of aeromonas sobria in aeromonas bacteremia. 19873805781
differentiation of motile and mesophilic aeromonas strains into species by testing for a camp-like factor.motile and mesophilic aeromonas strains can presumptively be differentiated into species in 18 to 24 h by testing the isolates for the production of a camp-like factor. aeromonas hydrophila strains were positive either aerobically or anaerobically, aeromonas sobria strains were positive only aerobically, and aeromonas caviae strains were always negative.19873611325
role of enterobius vermicularis in the aetiology of appendicitis.an evaluation was made of the histological material obtained from all 1529 appendices removed during the last 5 years at southmead hospital, bristol, in order to elucidate possible relationships between the incidence of enterobius vermicularis and the origin of inflammation in the appendix. in total, 1419 appendices were removed as cases of clinical appendicitis and 110 in the course of another surgical procedure. e. vermicularis was identified in 2.7 per cent of patients with clinical appendici ...19873651783
virulence markers of mesophilic aeromonads: association of the autoagglutination phenomenon with mouse pathogenicity and the presence of a peripheral cell-associated layer.autoagglutination (aa phenotype) of mesophilic aeromonads in broth was found to be a virulence-associated marker. there were two kinds of aa+ strains: those that spontaneously pelleted (sp+), and those that pelleted only after boiling (pab+). of 79 strains tested, 24 (30%) were aa+, and 18 of these were recovered from clinical specimens. most of the aa+ strains (n = 21) were identified as either aeromonas sobria or aeromonas hydrophila. of the well-documented clinical isolates of a. sobria and a ...19873679544
clearance of chlamydial elementary bodies from the conjunctival sac.the rate of disappearance of inactivated chlamydia trachomatis elementary body (eb) preparations from the conjunctival sac was studied in monkeys. direct fluorescent antibody (dfa) cytology showed that the majority of eb had been cleared from the eye within 24 hr of the inoculation of 1 x 10(6) inactivated eb, although small numbers of eb could be detected for up to 144 hr. the rate of clearance in normal and ocular immune animals did not differ, and formalin-killed and uv-inactivated ebs disapp ...19873298129
a practicable variant of the ion exchange method for the radiometric estimation of ornithine decarboxylase activity.a known ornithine decarboxylase assay working with ion exchange separation of [3h]ornithine and [3h]putrescine has been revised. the assay can be performed in disposable 1.5 ml vessels with a total of four pipetting steps. the separation of enzyme substrate and product, respectively, requires 3 h per 50 samples. the detection limit is about 50 pmoles [3h]putrescine formed.19873545892
Displaying items 1 - 100 of 544