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effect of acute ethanol administration on human blood aldehyde dehydrogenase activity.the aldehyde dehydrogenase (ec 1.2.1.3) activity in human blood was measured after acute oral administration of two different doses of ethanol to healthy subjects. no changes in aldehyde dehydrogenase activity were observed in either of the two dose groups, as compared to control subjects receiving no ethanol. likewise, incubation of human whole blood samples with 20 mm ethanol for 2 hr caused no change in aldehyde dehydrogenase activity. the results show that human blood aldehyde dehydrogenase ...19883067608
milk-feeding maintains the prochymosin production in cells of bovine abomasal mucosa.the effect of milk-feeding on the development of prochymosin-producing cells in bovine abomasal mucosa was studied using immunochemical methods. as long as the calf was fed milk both mucous neck, and chief cells in the gastric glands, were immunoreactive for prochymosin, the precursor form of a specific milk-clotting enzyme. after weaning, the prochymosin immunoreactivity in chief cells in the lower base of the gastric gland declined and finally disappeared. mucous neck cells and chief cells in ...19863083651
ammonia contamination in a milk processing plant--wisconsin. 19863084937
[a lipoma of the cerebellopontile angle].the case of a 38-year old man with a lipoma of the left cerebellopontine angle is presented. while the clinical signs of that tumour are unspecific, an exact diagnosis is possible by ct and mri. as in other reported cases only an incomplete removal of the tumours was made because of the adherence of the lipoma to the nerves of the cpa.19883261397
ammonia contamination in a milk processing plant--wisconsin. 19863084937
experimental models of type-i diabetes. 19863110756
neuroanatomy of memory and amnesia: a case for multiple memory systems.several different dissociable memory systems are considered in terms of their known or putative sites of pathology. in man, short-term memory can be selectively impaired by left hemisphere lesions near the acoustic projection area. there may be other modality-specific deficits. long-term memory is considered in terms of a) knowledge systems, b) stable associative memory; c) priming, and d) event memory, with special reference to the amnesic syndrome. for each of these categories, clinical neurop ...19873114179
functional domains of the yeast regulatory protein gal4.in the yeast saccharomyces cerevisiae regulation of the galactose/melibiose regulon rests on a dosage-dependent functional interplay between the positive regulator of transcription, the gal4 protein, and the negative regulator, gal80 protein. we have used this interplay to select in vitro generated fusions between the yeast adh1 promoter and gal4 coding sequence that overproduce gal4 protein, allowing the identification of gal4 protein in crude extracts from yeast. one type of these construction ...19862944111
ethical aspects of military physicians treating patients with hiv/part two: the duty to take initiative. 19883126439
effect of nitroglycerin on myocardial collateral conductance in awake dogs.conductance of the coronary collateral circulation during the course of two abrupt circumflex coronary occlusions (pre- and posttreatment with nitroglycerin) was measured in awake dogs approximately 2 wk after collateral vessels were stimulated to develop. the pressure gradient from the central aorta to the distal circumflex coronary artery was measured, and myocardial blood flow was determined by 9-microns radioactive microspheres at 30 s and 4 min after coronary occlusions. collateral conducta ...19883128122
serum albumin adducts in the molecular epidemiology of aflatoxin carcinogenesis: correlation with aflatoxin b1 intake and urinary excretion of aflatoxin m1.aflatoxin-serum albumin adducts in the blood of 42 residents of guangxi province, people's republic of china, were determined and compared with intake of aflatoxin b1 (afb1) and excretion of aflatoxin m1 (afm1) in urine. blood specimens were obtained during the same period that urine was collected and that diet was sampled. serum albumin was isolated from blood by affinity chromatography on reactive blue 2-sepharose and subjected to enzymatic proteolysis using pronase. immunoreactive products we ...19883133131
purification and characterization of salmolysin, an extracellular hemolytic toxin from aeromonas salmonicida.an extracellular hemolytic toxin of aeromonas salmonicida, termed salmolysin, was purified 945-fold by ammonium sulfate precipitation, anion-exchange chromatography on deae-cellulose, and gel filtration chromatography on sephadex g-100 and sepharose 2b. salmolysin appeared homogeneous upon cellulose acetate membrane electrophoresis and immunodiffusion analysis. the molecular weight of the toxin was estimated to be approximately 200,000 by the sedimentation equilibrium method. the uv absorption s ...19883136147
peripheral pmn cells in juvenile periodontitis. increased release of elastase and of oxygen radicals after stimulation with opsonized bacteria.in 12 patients with juvenile periodontitis (jp), stimulation of peripheral polymorphonuclear neutrophils (pmn) by opsonized bacteria showed increased release of free oxygen radicals and of elastase activity in relation to that of pair-matched healthy controls. the elastase increase was not associated with higher intracellular content of elastase, since the measurement of homogenized pmn cells did not differ between the patient group and the control group; nor did the spontaneous elastase activit ...19883165394
dna:dna reassociation analysis of aeromonas salmonicida.dna from 26 aeromonas salmonicida strains, namely 11 'typical' and 15 so-called 'atypical' strains, was used to assess the taxonomic relatedness within the species. the genomes were characterized by determination of dna base composition, dna:dna reassociation, calculation of sequence divergence following reassociation, and by genome size estimations. by comparison with dna obtained from controls and the aeromonas hydrophila group, a. salmonicida strains were determined to be correctly placed wit ...19883171541
the humoral immune response of rainbow trout (salmo gairdneri) immunised by various regimes and preparations of aeromonas salmonicida antigens.antibody production in rainbow trout to extracellular antigens was investigated. the following antigen preparations and immunisation regimes were used: native extracellular products (ecp) in freund's complete adjuvant (fca), intraperitoneally (i.p.) with and without booster; formalinized ecp in fca, i.p. with and without booster; washed, formalinized a. salmonicida cells in fca, i.p., with booster; native ecp in saline, i.m., four weekly injections at two different doses, 45 micrograms and 6 mic ...19883188377
isolation of aeromonas salmonicida from masu salmon in the republic of korea.the isolation and identification of the gram-negative fish pathogen, aeromonas salmonicida salmonicida, from masu salmon (oncorhynchus masou) at the yang yang salmon hatchery in the republic of korea is described. the bacterium possessed certain characteristics in common with isolates from fish in japan. it is an important cause of mortality among cultured salmonids in the republic of korea. this is the first report of this fish pathogen from the asian mainland.19883373645
selection for virulence in the fish pathogen aeromonas salmonicida, using coomassie brilliant blue agar.coomassie brilliant blue agar was used to quantify the frequency of the a-layer phenotype in different isolates of aeromonas salmonicida. hydrophilic, non-clumping isolates of a. salmonicida consisted predominantly of the a-layer minus phenotype. these bacteria were avirulent by intraperitoneal injection into susceptible brook trout (salvelinus fontinalis) and could not be reisolated from infected fish. by contrast, hydrophobic, clumping isolates were predominantly of the a-layer positive phenot ...19883193563
serological comparison between isolated strains of aeromonas salmonicida by agglutination and enzyme-linked immunosorbent assay (elisa). 19883206945
fulminant leukemic polyradiculoneuropathy in a case of b-cell prolymphocytic leukemia. a clinicopathologic report.a 52 year old man with a 10-month history of b-cell prolymphocytic leukemia (pll) died of an apparent acute fulminant polyradiculoneuropathy, a condition generally attributed to paraneoplastic complication. the pathologic examination disclosed diffuse leukemic infiltrations of the peripheral nervous system. it is suggested that this particularly aggressive form of b-cell chronic prolymphocytic leukemia presented a constellation of features that promoted the invasion of the peripheral nervous sys ...19873040217
cloning of the gene for the surface array protein of aeromonas salmonicida and evidence linking loss of expression with genetic deletion.a gene bank of dna from the fish pathogenic bacterium aeromonas salmonicida was constructed in the bacteriophage lambda gt11. phage lambda gt11/10g, a recombinant carrying a 4.0-kilobase fragment of a. salmonicida dna, was found to express the surface array protein (a protein) in escherichia coli. sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the protein expressed from the cloned gene had a subunit molecular weight of 49,000, which was identical to that of subunits in the ...19873040676
differential susceptibility of salmonid fishes to furunculosis correlates with differential serum enhancement of aeromonas salmonicida extracellular protease activity.rainbow trout, atlantic salmon and brown trout, respectively, exhibit an increasing order of susceptibility to furunculosis. we investigated if this may be due to a differential sensitivity to exotoxins or ability to inhibit the exoprotease which is required by the bacterium to digest protein for growth. differences were slight in susceptibility to exotoxins and serum inhibition of exoprotease at high exotoxin:serum ratios. however, at low ratios brown trout serum markedly enhanced protease acti ...19883059141
modulation by cytosolic ph of calcium and rubidium fluxes in rat pancreatic islets.cytosolic ph (phi) of pancreatic islet cells was assessed using the fluorescent dye 2'7'-biscarboxyethyl-5'(6')-carboxyfluorescein (bcecf). phi was rapidly lowered by addition of the sodium salt of a weak acid or by treatment with amiloride. in the latter case, no recovery of phi occurred. nh4cl produced a rise in phi. stimulation of islet cells with glyceraldehyde produced a sustained fall in phi, whereas glucose and alpha-ketoisocaproate caused a small, gradual rise in phi. intracellular acidi ...19883060124
retroviral transfer of genes into canine hematopoietic progenitor cells.amphotropic retroviral vectors containing either the bacterial neomycin phosphotransferase gene or a mutant dihydrofolate reductase gene (dhfr*) were used to infect canine hematopoietic progenitor cells. successful transfer and expression of both genes in canine hematopoietic progenitor cells has been achieved as measured by the ability of the viruses to confer resistance to either methotrexate (mtx) or the aminoglycoside g418, respectively. gene transfer was achieved using helper-free retrovira ...19883066170
differential cardiovascular effects of central clonidine and b-ht 920 in conscious rats.the effect of intracerebroventricular (i.c.v.) injection of the alpha 2-adrenoceptor agonists clonidine and b-ht 920 on mean arterial pressure (map), heart rate (hr), and plasma concentrations of noradrenaline and adrenaline was examined in conscious unrestrained rats. the injection of 1.0 microgram clonidine significantly decreased map and slightly decreased hr. plasma noradrenaline and adrenaline levels were slightly but not significantly decreased after the injection of 1 microgram clonidine. ...19883242780
protein turnover and growth of l8 muscle cultures in serum from rats fed clenbuterol.beta-adrenergic agonists stimulate muscle growth in chronically treated animals. the response may be primary (receptor binding directly stimulates anabolism) or secondary (circulating levels of hormones or other factors regulate muscle mass). although b-receptors are functional in l8 muscle cells in culture, a direct effect of b-agonists on protein turnover in culture has not been demonstrated. to determine whether the stimulation of muscle growth is a secondary effect, we assessed the anabolic ...19883255725
[a lipoma of the cerebellopontile angle].the case of a 38-year old man with a lipoma of the left cerebellopontine angle is presented. while the clinical signs of that tumour are unspecific, an exact diagnosis is possible by ct and mri. as in other reported cases only an incomplete removal of the tumours was made because of the adherence of the lipoma to the nerves of the cpa.19883261397
[clinical results of rokitamycin dry syrup on respiratory tract infections in pediatric field].1. a total of 15 patients with pediatric respiratory tract infections were mediated with rokitamycin (rkm) dry syrup, and its clinical effects were excellent in 2, good in 10, fair in 1 and poor in 1. 2. the efficacy rate in streptococcus pneumoniae pneumonia and mycoplasmal pneumonia was 100%. 3. clinical effects on 7 patients with haemophilus influenzae were excellent in 1, good in 4, fair in 1 and poor in 1. 4. no adverse reaction was observed, and the taste and odor of rkm was well accepted ...19883262780
regulation of type i collagen synthesis by 1,25-dihydroxyvitamin d3 in human osteosarcoma cells.synthesis of type i and iii collagens has been examined in mg-63 human osteosarcoma cells after treatment with the steroid hormone, 1,25-dihydroxyvitamin d3 (1,25-(oh)2d3). analysis of total [3h]proline-labeled proteins and pepsin-derived collagens revealed that 1,25-(oh)2d3 selectively stimulated synthesis of alpha 1i and alpha 2i components of type i collagen after 6-12 h. consistent with previous reports (franceschi, r. t., linson, c. j., peter, t. c., and romano, p. r. (1987) j. biol. chem. ...19883264282
purification of infectious canine parvovirus from cell culture by affinity chromatography with monoclonal antibodies.immuno affinity chromatography with virus neutralizing monoclonal antibodies, directed to the haemagglutinating protein of canine parvovirus (cpv) was used to purify and concentrate cpv from infected cell culture. the procedure was monitored by testing the respective fractions in an infectivity titration system, in an elisa, in a haemagglutination assay and by negative contrast electron microscopy to quantify cpv or cpv antigen. the degree of purification was further estimated by testing the fra ...19873294877
analysis of structure-activity relationships in renin substrate analogue inhibitory peptides.on the basis of the minimal octapeptide sequence of the renin substrate, a series of peptides was synthesized containing (3s,4s)-4-amino-3-hydroxy-6-methylheptanoic acid (statine) or (3s,4s)-4-amino-3-hydroxy-5-phenylpentanoic acid (ahppa) at the p1p1' position. some of these peptides also contained nin-formyltryptophan at the p5, p3, or p3' position. renin-inhibitory potency varied over a wide range (from inactive to ic50 = 3 nm). potency was reduced by at least 10-fold when the peptide was sho ...19873302256
amelioration of the ischemic damage of the dog kidney subjected to 90 minutes of warm ischemia by lidocaine pretreatment and low-dose dopamine infusion on revascularization. 19873303853
effects of long term oral administration of ketanserin and trichlormethiazide on blood pressure in stroke-prone spontaneously hypertensive rats. 19883240734
effects of long term oral administration of ketanserin and trichlormethiazide on blood pressure in stroke-prone spontaneously hypertensive rats. 19883240734
norepinephrine is a presynaptic input to visual corpus callosum cells.the neurochemical identity of presynaptic inputs to cell bodies of corpus callosum fibers (callosal cells) located in visual cortex of the cat was investigated using a double labeling technique. callosal cells were labeled by horseradish peroxidase applied to the severed ends of callosal fibers, and [3h]norepinephrine (ne) was injected into the crown of the lateral gyrus corresponding to the visual cortical area 17/18 border to label synapses. the tissue surrounding the injection sites was proce ...19883340321
the processing of asparagine-linked oligosaccharides in ht-29 cells is a function of their state of enterocytic differentiation. an accumulation of man9,8-glcnac2-asn species is indicative of an impaired n-glycan trimming in undifferentiated cells.studies on the regulation of the enterocytic differentiation of the human colon cancer cell line ht-29, which is differentiated in the absence (glc-) but not in the presence of glucose (glc+), have recently shown that the post-translational processing of sucrase-isomaltase and particularly its glycosylation vary as a function of cell differentiation (trugnan g., rousset, m., chantret, i., barbat, a., and zweibaum, a. (1987) j. cell biol. 104, 1199-1205). other studies indicate that in undifferen ...19883360773
evaluation of prostaglandins as mediators of tubuloglomerular feedback.orthograde and retrograde microperfusion experiments were conducted in sprague-dawley rats to evaluate the participation of vasoconstrictive eicosanoids as mediators of tubuloglomerular feedback (tgf) signals. retrograde perfusion with 160 microm arachidonic acid (aa) added to a hypotonic solution enhanced the stop-flow pressure (sfp) feedback responses compared with those obtained with the control hypotonic solution (delta sfp, 1.6 +/- 0.4 vs. 10.1 +/- 0.7 mmhg with aa). blockade of thromboxane ...19883364575
a comparison of the distribution of extracellular proteins produced by the protease-secreting organism aeromonas salmonicida during aerobic and anaerobic growth.aeromonas salmonicida was grown aerobically and anaerobically in supplemented 3% (w/v) tryptone soya broth medium for 24 h at 25 degrees c. although the bacterial density achieved was 4.9 times higher in the aerobic culture, the exoprotein produced per unit of bacterial dry weight was only 1.9 times higher than in the anaerobic culture. however, the protease activity of the exoprotein showed a marked reduction anaerobically, being only one-tenth of that of the exoprotein produced aerobically. th ...19863322167
a comparative study of the formation of extracellular proteins by aeromonas salmonicida at two different temperatures.aeromonas salmonicida was grown in a supplemented 3% (w/v) tryptone soya broth medium at 10 degrees c, a temperature at the lower end of the range over which furunculosis has been observed to occur in the field, and 25 degrees c, the optimum temperature for growth. similar bacterial densities in the range 2.35 +/- 0.05 mg dry wt/ml were achieved in the two cultures at the beginning of the stationary phase of the growth cycle, after 125 h at 10 degrees c and 18 h at 25 degrees c. at this point, a ...19873298187
increasing use of smokeless tobacco leads to fears of young lives being 'snuffed out'. 19883411725
hiv-1 seroprevalence in male prostitutes in northeast italy. 19883216323
application of transmission electron microscopy to human tumor diagnosis: an historical perspective. 19873322513
experimental contact sensitization with 3,4,5-trichloropyridazine.the potential of 3,4,5-trichloropyridazine to induce contact sensitization was assessed in the guinea pig maximization test of magnusson and kligman and also in the closed patch test described by buehler. the test material was a 1% solution of 3,4,5-trichloropyridazine in a highly refined mineral oil. the test material elicited moderate to severe irritation when diluted in mineral oil to concentrations of 15-25% and minimal irritation at concentrations of 1-3%. both tests clearly indicated that ...19883416588
on the increased retention volume of human haemoglobin in high-performance gel filtration. 19873429527
a procedure of vestibular decompensation for clinical diagnosis. i. animal experiments.vestibular lesions are often so effectively compensated that their diagnosis may be difficult. certain chemicals can decompensate the central vestibular system and thus uncover symptoms present before compensation. this study attempts to devise a procedure which can utilize such decompensation for the benefit of clinical diagnosis. unilateral labyrinthectomies were performed in rats. pathological movements and body positions due to the vestibular loss were compensated within about one week. syst ...19863485723
polarity reversal of inside-out thyroid follicles cultured within collagen gel: structure of the junctions assessed by freeze-fracture and lanthanum permeability.the organization of tight junctional complexes (tjs) was studied in cultured porcine thyroid cells during the inversion of polarity induced by collagen-embedding of inside-out follicles, using freeze-fracture replicas and lanthanum penetration. during the early steps of polarity reversal, freeze-fractures showed that tjs generally persisted. they increased in width and progressively branched out into the basolateral surfaces, towards the basal pole. later, the number of tj strands decreased and ...19883390623
vaccination against cutaneous leishmaniasis in a murine model. ii. immunologic properties of protective and nonprotective subfractions of soluble promastigote extract.we have previously demonstrated that balb/c mice can be protected against a fatal infection with leishmania major by i.p. immunization with a soluble leishmanial antigen (sla) preparation in conjunction with the adjuvant, corynebacterium parvum (cp). in this study, sla was separated into nine distinct fractions by anion exchange liquid chromatography, and the fractions were analyzed for their ability to stimulate t cells obtained from immunized mice, to be recognized by vaccine-induced antibodie ...19873499465
[anisodamine in the treatment of 647 cases of severe hepatitis]. 19883416397
[a new method for the etiological diagnosis of human scabies]. 19873504153
multiple low-level antibiotic resistance in aeromonas salmonicida.mutants with multiple low-level antibiotic resistance were isolated from virulent wild-type aeromonas salmonicida strains exposed to a low concentration of any one of several low-molecular-mass (approximately 635 daltons or less) antibiotics. multiple resistance was toward beta-lactam compounds (penicillin g, ampicillin, cloxacillin), quinolones (flumequine, oxolinic acid, nalidixic acid), tetracyclines, chloramphenicol, and novobiocin. susceptibilities of the mutants toward several higher-molec ...19863524440
the defect of lymphocyte locomotion in chronic lymphocytic leukaemia: studies of polarization and growth-dependent locomotion.this paper reports a study of the locomotor behaviour of the lymphocytes from 17 patients with chronic lymphocytic leukaemia (cll). the cells were studied both immediately after separation from blood and after culture for 24 to 48 h with a range of growth activators. cells direct from blood were tested for polarization in fetal calf serum (fcs 20%), phorbol myristate acetate (pma 10(-7)m) and colchicine (10(-5)m). the polarization of lymphocytes from cll patients with high white cell (wbc) count ...19883383451
pharmacokinetics of insulin following intravenous and subcutaneous administration in canines.studies were conducted to examine the absorption and disposition kinetics of insulin in dogs following intravenous (iv) and subcutaneous (sc) administration of commercial preparations. after iv and sc dosing, the plasma levels were described by models which considered basal insulin level contributions. intersubject variation in the disposition kinetics was small with half-lives of 0.52 +/- 0.05 h and total body clearances of 16.21 +/- 2.08 ml min-1 kg-1. calculated insulin plasma secretion rates ...19863535917
diagnosis of plasmodium falciparum infection by spot hybridization assay: specificity, sensitivity, and field applicability.the spot hybridization assay for the detection of plasmodium falciparum reported here uses as probe a repetitive dna sequence from this species and exhibits a high degree of species specificity. isolates from african, asian, and south american patients were positive in the assay and gametocytes could be detected at the same level of parasitaemia as asexual parasites. an rna probe containing the same repetitive sequence as the dna probe has a detection limit of 1 parasite per 10(6) red blood cell ...19863536140
residual nitrobenzylthioinosine-resistant nucleoside transport in a transport mutant (ae1) of s49 murine t-lymphoma cells.the uptake of various nucleosides by s49 mouse t-lymphoma cells and that by a single-step nucleoside transport-defective mutant thereof (ae1) were compared. residual nucleoside entry into ae1 cells occurred via two routes, nonmediated permeation and saturable, non-concentrative transport with broad substrate specificity and a michaelis-menten constant approximating that for thymidine transport in wild-type cells. however, in contrast to nucleoside transport in wild-type cells, residual nucleosid ...19873494190
aeromonas salmonicida from salmonids and cyprinids--serological and cultural identification. 19863551409
identification of major common extracellular proteins secreted by aeromonas salmonicida strains isolated from diseased fish.ten different strains of aeromonas salmonicida that were isolated from diseased fish were grown under identical conditions (24 h at 25 degree c) in 3% (wt/vol) tryptone soya broth medium supplemented with vitamins and inorganic ions. in each case the extracellular proteins that were formed were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and it was found that there were two significant common components, one with a molecular weight of 70,000 and the other with a weight ...19873555336
further improvement of high-speed nmr flow-velocity measurement using a differential phase-encoding technique.an nmr imaging technique applicable to high-velocity flow imaging is described. by subtraction of two phase images obtained by varying the flow-encoding gradient, it is possible to extract much larger ranges of velocity components. this differential phase-encoding technique is tested by experiments in a phantom as well as a human volunteer.19873574062
enhancement of neutrophil-mediated phagocytosis by human granulocyte-macrophage colony-stimulating factor demonstrated using a novel mathematical model.a mathematical model is presented which may be applied to describe and analyse data from microscopic phagocytosis assays. the method has been used to investigate the phagocytosis of opsonized yeast by peripheral blood neutrophils treated with purified recombinant human granulocyte-macrophage colony-stimulating factor (rh gm-csf) in vitro. under limiting conditions of serum opsonization, rh gm-csf decreased the proportion of non-phagocytic cells and increased the mean number of ingested yeast per ...19873500121
heterogeneous enzyme immunoassay: detection of anti-candida albicans specific ig-isotypes and mannan antigen. 19863534563
virulence, cytotoxic and inflammatory activities of vibrio anguillarum and aeromonas salmonicida isolated from cultivated salmonid fish in sweden. 19873687646
loss of virulence in a protease-deficient mutant of aeromonas salmonicida.the importance of extracellular protease production by aeromonas salmonicida, the bacterial pathogen of fish furunculosis, was investigated with four virulent strains (which were autoagglutinative, hemagglutinative, resistant to fish serum, adhesive to fish tissue culture, protease positive, hemolysin positive, and leukocytolysin positive) and three avirulent strains (which were nonagglutinative, nonhemagglutinative, sensitive to serum, nonadhesive, protease positive, hemolysin positive, and leu ...19853884507
isotope exchange at equilibrium studies with rat muscle adenylosuccinate synthetase.the kinetic mechanism of rat muscle adenylosuccinate synthetase was studied by determining the rates of isotope exchange at equilibrium. a random sequential binding mechanism was indicated for both the forward and reverse reactions. aspartate, adenylosuccinate, gdp, and pi were determined to bind in rapid equilibrium. gtp exchanges with both gdp and pi at the same rate, which is similar to the exchange rate of imp with adenylosuccinate. aspartate exchanges with adenylosuccinate at a higher rate ...19863542024
preparation and properties of fabigg, a chimeric univalent antibody designed to attack tumour cells.in order to promote the killing of tumour cells by antibody a derivative has been synthesized in which fab'gamma from xenogeneic antibody is thioether-bonded to half-cystine on normal igg of the species to be treated. the resulting entity, fabigg, is obtained with about a 40% yield of the starting fab'gamma. being univalent it evades antigenic modulation. it activates complement efficiently, is minimally immunogenic, and appears to be catabolized at the slow rate characteristic of autologous igg ...19853879458
surface properties of autoagglutinating mesophilic aeromonads.the surface characteristics of 24 autoagglutinating (aa+) mesophilic aeromonads were investigated. one group of 16 was found to be highly related serologically by their reactive pattern against o antisera generated against three reference strains. subsequent characterization of 11 of these isolates (group 1) indicated that they had the following properties in common: precipitation after boiling (pab+), membership of serogroup o:11 (typing scheme of sakazaki and shimada), resistance to lysis by b ...19883417353
[involvement of the growth hormone in rapid increases of the potassium concentration of the serum].as a rule, the infusion of arginine hydrochloride is followed by an increase in serum potassium. the investigation is concerned with the short-term effect of growth hormone, which can be stimulated by arginine (0.6 g/kg body weight), on potassium concentrations in rats and patients with cerebral death. the administration of human somatotropin causes a significant rise in potassium concentrations in patients with cerebral death (0.4 iu/kg body weight) and in rats (4.0 iu/kg body weight). in compa ...19873439594
two patterns in the aeromonas salmonicida a-layer may reflect a structural transformation that alters permeability.electron micrographs of negatively stained regular surface layers (a-layers) of aeromonas salmonicida showed two square patterns having p4 symmetry. computer image processing demonstrated that, at a resolution of 2.3 nm, both square arrays were composed of two different morphological units arranged alternatively to give a face-centered lattice in which the four nearest neighbors of each unit were the other type of unit. the lattice constant was slightly but significantly different in the two pat ...19863957865
blood changes in carp (cyprinus carpio) induced by ulcerative aeromonas salmonicida infections.only recently aeromonas salmonicida has been recognized as a significant bacterial pathogen in ulcerative disease of cyprinid fish. our attempts to formulate a vaccine based on bacterial surface antigens were unsuccessful in conferring reliable protection against lethal challenge. this lead us to study pathological changes in the humoral defense system during ulcerative a. salmonicida infection in carp. high numbers of opportunist pathogens such as a. hydrophila and pseudomonas sp. were frequent ...19863765352
does aeromonas salmonicida affect the immune system of carp, cyprinus carpio l.?aeromonas salmonicida is a significant bacterial pathogen of cyprinid and salmonid fishes causing the systemic disease furunculosis. several observations led us to believe that a. salmonicida was able to evade or suppress the immune system of the fish: injection of whole bacteria or surface antigens was unsuccessful at protecting fish against lethal challenges; memory did not develop in survivors of sublethal infections; diseased fish often carried other opportunistic bacterial pathogens in addi ...19863765353
age-dependent activation of glucocorticoid receptors in the cerebral hemispheres of male rats.the binding of [3h]dexamethasone-receptor complexes to purified nuclei was studied in the cerebral hemispheres of immature (3-week-old) and mature (26-week-old) long-evans male rats to determine the age-related changes, if any, in the physicochemical properties of glucocorticoid receptors. our data show that heat activation (for 45 min at 25 degrees c) significantly enhances the nuclear binding of [3h]dexamethasone-receptor complexes in rats of both ages, with a greater magnitude in immature rat ...19873690338
a modified enzyme linked immunosorbent assay (elisa) for monitoring antibody production during experimental aeromonas salmonicida infection in rainbow trout (salmo gairdneri) 19863770272
detection of fish antibody against protein antigen of aeromonas salmonicida by enzyme-linked immunosorbent assay using biotin-avidin system.antibody against aeromonas salmonicida was detected in sera from immunised or experimentally infected rainbow trout by enzyme-linked immunosorbent assay (elisa) using the biotin-avidin system. the elisa titre correlated well with the agglutinin titres of the sera, but the elisa was found to be more sensitive than the agglutination test. when the rainbow trout serum was separated by column chromatography, antibody activity (determined by elisa and agglutination test) was detected in the igm fract ...19873628989
microsomal binding sites for antioestrogens in rat liver. properties and detergent solubilization.the properties of an antioestrogen binding site (aebs), which has high affinity and specificity for nonsteroidal antioestrogens and structurally related compounds, have been studied in rat liver microsomes. when subcellular organelles were separated on percoll density gradients the distribution of the aebs paralleled that of nadph-cytochrome c reductase, indicating that the aebs is associated with the endoplasmic reticulum. saturation analysis showed that [3h]tamoxifen was bound to a single clas ...19863790095
opiate microinjections into midbrain do not affect the aversiveness of caudal trigeminal stimulation but produce somatotopically organized peripheral hypoalgesia.high-dose microinjections of morphine sulfate (15 micrograms) and (d-ala2)-met-enkephalin (30 micrograms) were made into the ventral periaqueductal gray of rats. consistent with previous reports using lower doses, both opiates produced hypoalgesia for noxious thermal stimuli applied to the upper and lower body. more hypoalgesia was observed on the face than on the hind legs or tail. current thresholds of aversive reaction to stimulation in the trigeminal subnucleus caudalis were unaffected by mi ...19863768692
the structure of the heptose-3-deoxy-d-mannooctulosonic-acid region in a mutant form of aeromonas salmonicida lipopolysaccharide.lipopolysaccharide was isolated from a phage-selected mutant of a wild strain of aeromonas salmonicida by the aqueous phenol method. the lipopolysaccharide consisted of the r form, containing per mole, three moles of l-glycero-d-manno-heptopyranose, one mole of 3-deoxy-d-manno-2-octulosonic acid (docla) and lipid a. the docla was not fully assayable by the thiobarbituric acid methods usually used, but its degradation product was detected, after smith degradation of the lipopolysaccharide, either ...19863780744
presence of glucocorticoid receptors in cultured thymic epithelial cells.we investigated the presence of glucocorticoid receptors (gc) in human thymic epithelial cells grown in primary cultures and in a pure epithelial rat cell line. these gr levels were compared to those determined concomitantly in fresh human thymocytes. the average number of sites were 54,457/cell for males (n = 8) and 58,224/cell for females (n = 8) with mean kd values of 1.5 and 1.7 x 10(-8) m, respectively, in cultured human epithelial cells. these results are comparable to those obtained for r ...19863742599
growth response of mouse lymphoma cells to low concentrations of mercuric chloride.three growth rate experiments involving several sampling points were performed to investigate the previous finding that very low concentrations of hgcl2 inhibit the growth of murine lymphoblasts in vitro. however, results presented here do not confirm this, there being no significant differences between the three independent growth rate experiments.19853832669
adhesion of aeromonas salmonicida strains associated with net electrostatic charges of host tissue cells.the adhesion of aeromonas salmonicida, the pathogenic bacterium of fish furunculosis in salmon and trout, to the surface of host tissue cells was investigated with two fish tissue culture cell lines (rtg-2 cells from rainbow trout, salmo gairdneri, and chse-214 cells from chinook salmon, oncorhynchus tshawytscha) and four a. salmonicida strains. bacterial cells of pathogenic strains were highly adhesive to rtg-2 and chse-214 cells and were negatively charged in the net electrostatic charges, as ...19873818093
evaluation of growth fractions with monoclonal antibodies to human alpha-dna polymerase.we have established and partially characterized a panel of monoclonal antibodies against alpha-dna polymerase. one of the hybridomas, clone 5f, has been exploited for cell kinetic studies on three colon cancer cell lines, lovo, sw 620, and sw 403, which are endowed with different growth patterns and differentiation status. by an immunoperoxidase method, we could demonstrate the specific intranuclear localization of alpha-dna polymerase during the exponential phase of in vitro growth and contrast ...19873815379
evaluation of growth fractions with monoclonal antibodies to human alpha-dna polymerase.we have established and partially characterized a panel of monoclonal antibodies against alpha-dna polymerase. one of the hybridomas, clone 5f, has been exploited for cell kinetic studies on three colon cancer cell lines, lovo, sw 620, and sw 403, which are endowed with different growth patterns and differentiation status. by an immunoperoxidase method, we could demonstrate the specific intranuclear localization of alpha-dna polymerase during the exponential phase of in vitro growth and contrast ...19873815379
deficiency in 15-hydroxyprostaglandin dehydrogenase activity after unilateral ureteral obstruction of the dog kidney.in the present study, metabolism of prostaglandins (pgs) by 15-hydroxyprostaglandin dehydrogenase (15-oh pgdh) was investigated in dog kidneys with ureteral obstruction. after 24 hr of ureteral obstruction, the obstructed kidney and the contralateral kidney were removed and the cytosolic fractions (105,000 x g), enriched in 15-oh pgdh, were prepared from the cortex and medulla. 15-oh pgdh activity was estimated by radiometric assays of the metabolism of [3h]pge2 and [3h]prostacyclin. cortical 15 ...19853881582
role of caseinase from aeromonas salmonicida in activation of hemolysin.mutants of the bacterial fish pathogen aeromonas salmonicida selected for inability to digest casein concomitantly lost hemolytic activity against horse erythrocytes under certain conditions. mixtures of wild-type with mutant culture supernatants indicated that mutants produce an inactive precursor of a hemolysin which was activated by autogenous caseinase and, with less efficiency, by other serine proteases. selective inhibition or repression of caseinase production in the wild-type strain also ...19853897063
localization in the kidney of antibodies to human skin and malignant melanoma. 19664161935
induction of proliferation in vitro of resting human natural killer cells: expression of surface activation antigens.in this report, we show that resting human peripheral blood nk cells, positively enriched with antibody b73.1, can be induced to proliferate in vitro in short-term cultures, and newly express membrane activation antigens. b73.1+ nk cells, cultured for 6 days in the presence of conditioned medium containing il 2 and irradiated b lymphoblastoid cells, show significant [3h]tdr incorporation beginning on day 4. at that time, a large proportion of the cells express hla-dr and 4f2 antigens, and transf ...19853917474
porphyrin binding by the surface array virulence protein of aeromonas salmonicida.congo red binding by virulent a-layer-containing (a+) and avirulent a-layer-deficient (a-) strains of aeromonas salmonicida was examined. congo red binding to a+ cells was enhanced by salt and thus hydrophobically driven, but at low congo red concentrations binding was salt independent. congo red was bound by a+ cells by a kinetically distinct mechanism (kd, 0.25 microm) which was absent in a- isogenic strains. purified a-layer protein ("a protein") protein a also bound congo red with similar af ...19853877720
on the active site of myosin a-adenosine triphosphatase. x. functions of two subfragments, s-1, of the myosin molecule. 19704249516
role of sulfate conjugation of catecholamines in blood pressure regulation.catecholamine sulfates were found to be inactive at vascular receptor sites. only at one nonvascular receptor site important for blood pressure (bp) regulation was dopamine-3-o-sulfate found to be an inhibitory modulator of the adrenocortical secretion of aldosterone in vitro. however, sulfation of catecholamines (ca) does play an important role in bp regulation, as shown by the following observations: sulfoconjugated ca with a long half-life are sometimes better markers of ca release than free ...19863720964
spontaneous craniopharyngioma in a mouse. 19863750744
identification of neuropeptide y immunoreactivity in the suprachiasmatic nucleus and the lateral geniculate nucleus of some mammals.the distribution of neuropeptide y (npy) immunoreactivity in the suprachiasmatic nucleus (scn) and the lateral geniculate nucleus (lgn) of 5 kinds of mammals was studied using the peroxidase-anti-peroxidase method. in the scn of the rat, hamster, chipmunk and cat, npy-immunoreactive fibers were detected, particularly in the ventral part. in the 3 rodents and the cat, the somata and fibers of npy-immunoreactive neurons were observed in the lgn. however, immunoreactive structures were not found in ...19863755229
interaction between aeromonas salmonicida and peritoneal macrophages of brook trout (salvelinus fontinalis).the phagocytic and bactericidal properties of peritoneal macrophages obtained from brook trout (salvelinus fontinalis), injected with either glycogen or a modified freund's complete adjuvant (mfca), were evaluated against an avirulent and a virulent strain of aeromonas salmonicida. avirulent bacteria were effectively phagocytized and killed by macrophages obtained from fish injected with both irritants. with glycogen-elicited macrophages, no enhancement of killing was observed following opsoniza ...19863765344
effect of an immunopotentiator on aeromonas salmonicida infection in rainbow trout (salmo gairdneri).the immunoactive peptide fk-565 (heptanoyl-y-d-glutamyl-(l)-mesodiaminopimelyl-(d)-alanine) was found to induce protection against intraperitoneal aeromonas salmonicida infection in rainbow trout (salmo gairdneri richardson). the survival rate was as high as 60% when fk-565 was given intraperitoneally as a single dose (1mg/kg) one day before bacterial challenge. a non-specific stimulation of phagocytic cells by fk-565 at an early stage of the bacterial infection may contribute to the resistance ...19863765348
comparison of immune status and 1,2-dimethylhydrazine induced tumorigenesis in brown--norway and fischer rats. emphasis on splenic and colonic lymphocyte function.sym 1,2-dimethylhydrazine (dmh)-induced colon tumorigenesis was studied in immunologically different strains of rat: the brown--norway which is known to be immunologically a low-responder and the fischer a high-responder. brown--norway rats received a total dose of 75, 150 or 225 mg dmh/kg or vehicle and fischer rats received 150 mg dmh/kg or vehicle over a 3-week period. rats were killed 5 months after the final treatment. lymphocytes were isolated from the spleen and colon from rats treated wi ...19853871659
[effect of titanium phosphate, zirconium oxide and a mixture of ammonium phosphotungstenate with zirconium phosphate on the elimination of radioactive cesium from the bodies of rats]. 19853835674
metabolic and morphological alterations of brown adipose tissue after sympathetic denervation in rats.effects of sympathetic denervation on biochemical and morphological changes of brown adipose tissue (bat) were studied in rats after severing 5 intercostal nerve bundles that enter the unilateral interscapular bat, the contralateral bat being used as the control. four weeks after denervation, there was no appreciable change in bat weight or its dna and protein contents, whereas the triglyceride content was increased significantly. however, the rate of fatty acid synthesis was decreased to about ...19863958437
responses of eeg, cerebral oxygen consumption and blood flow to peripheral nerve stimulation during thiopentone anaesthesia in the dog.the effects of sciatic nerve stimulation on the electroencephalogram (eeg), cerebral metabolic rate for oxygen (cmro2) and cerebral blood flow (cbf) were investigated during thiopentone anaesthesia in dogs. anaesthetic levels at 15, 35, 65, 95 and 125 minutes after the start of thiopentone infusion (23 mg x kg-1 x hr-1) were designated levels i, ii, iii, iv and v, respectively. the effects of stimulation for 5 min were tested at each level. at level i (plasma thiopentone concentration; 15 +/- 2 ...19853930049
role of sulfate conjugation of catecholamines in blood pressure regulation.catecholamine sulfates were found to be inactive at vascular receptor sites. only at one nonvascular receptor site important for blood pressure (bp) regulation was dopamine-3-o-sulfate found to be an inhibitory modulator of the adrenocortical secretion of aldosterone in vitro. however, sulfation of catecholamines (ca) does play an important role in bp regulation, as shown by the following observations: sulfoconjugated ca with a long half-life are sometimes better markers of ca release than free ...19863720964
congo red agar, a differential medium for aeromonas salmonicida, detects the presence of the cell surface protein array involved in virulence.strains of the fish pathogen aeromonas salmonicida which possess the cell surface protein array known as the a-layer (a+) involved in virulence formed deep red colonies on tryptic soy agar containing 30 micrograms of congo red per ml. these were readily distinguished from colorless or light orange colonies of avirulent mutants lacking a-layer (a-). the utility of congo red agar for quantifying a+ and a- cells in the routine assessment of culture virulence was demonstrated. intact a+ cells adsorb ...19853934141
a comparative examination of the in vitro metabolism of five cyclopenta[a]phenanthrenes of varying carcinogenic potential.metabolites of 15,16-dihydrocyclopenta[a]phenanthren-17-one and its 1- and 12-methyl homologues (all non-carcinogens) along with those from the 11-methyl and 11,12-dimethyl-17-ketones (carcinogens), produced in vitro by hepatic microsomes from methylcholanthrene induced rats, were separated by reverse phase h.p.l.c. identifications of individual metabolites were based upon elution times, u.v. spectra, and in some cases by mass spectrometry, circular dichroism, and identity with synthetic derivat ...19854017190
a comparative examination of the in vitro metabolism of five cyclopenta[a]phenanthrenes of varying carcinogenic potential.metabolites of 15,16-dihydrocyclopenta[a]phenanthren-17-one and its 1- and 12-methyl homologues (all non-carcinogens) along with those from the 11-methyl and 11,12-dimethyl-17-ketones (carcinogens), produced in vitro by hepatic microsomes from methylcholanthrene induced rats, were separated by reverse phase h.p.l.c. identifications of individual metabolites were based upon elution times, u.v. spectra, and in some cases by mass spectrometry, circular dichroism, and identity with synthetic derivat ...19854017190
studies on tissue distribution of glutathione and on activities of glutathione-related enzymes after carbon tetrachloride-induced liver injury.after an initial decrease following ccl4-application (1.0 ml/kg bodyweight, i.p.), in the liver of male wistar rats, values of reduced glutathione (gsh) are at a maximum 6 and 12 h after treatment. oxidized glutathione (gssg) increases up to 24 h after intoxication. malondialdehyde (mda) is at a low level, when gsh is high. glutathione peroxidase has a clear initial increase of activity, but otherwise shows only slight deviations from control values. cytosolic and microsomal glutathione-s-transf ...19853992016
immunity to aeromonas salmonicida in coho salmon (oncorhynchus kisutch) induced by modified freund's complete adjuvant: its non-specific nature and the probable role of macrophages in the phenomenon.juvenile coho salmon (oncorhynchus kisutch), vaccinated with one intraperitoneal injection of formalin-killed virulent aeromonas salmonicida cells suspended in saline, showed increased protection against approximately one ld60 of homologous challenge administered at 30 days post-vaccination. under similar conditions, coho vaccinated with a modified complete freund's adjuvant (mfca) alone were also equally protected. when measured against a more severe a. salmonicida challenge of approximately on ...19854043481
the purification and some properties of h-lysin from aeromonas salmonicida.h-lysin from aeromonas salmonicida has been purified 1770-fold by freeze fractionation, ammonium sulphate precipitation, ion exchange chromatography and gel filtration chromatography. the purified material was predominantly h-lysin, devoid of detectable t-lysin, caseinase or gelatinase activity, although glycerophospholipid: cholesterol acyltransferase (gcat) activity was present. the results suggested that h-lysin and gcat activities were due to different extracellular products. studies of the ...19854045422
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