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production of p(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate) terpolymers using a chimeric pha synthase in recombinant ralstonia eutropha and pseudomonas putida.recombinant strains of ralstonia eutropha and pseudomonas putida harboring a chimeric polyhydroxyalkanoate (pha) synthase, which consisted of pha synthases of aeromonas caviae and r. eutropha, produced 3-hydroxybutyrate (3hb)-based pha copolymers comprised of 3-hydroxyhexanoate and 3-hydroxyoctanoate units from dodecanoate (87-97 mol % 3hb), indicating that the chimeric pha synthase possesses desirable substrate specificity leading to the production of 3hb-rich copolymers.201020699558
dynamics of aeromonas species isolated from wastewater treatment system.aeromonas are widely distributed in the aquatic environment, and are considered to be emerging organisms that can produce a series of virulence factors. the present study was carried out in a sanitary sewage stabilization pond treatment system, located in lins, state of são paulo, brazil. most probable number was applied for estimation of the genus aeromonas. colony isolation was carried out on blood agar ampicillin and confirmed by biochemical characterization. aeromonas species were isolated i ...201020705981
evaluation of a bwtk for detection of total coliforms, e. coli and emerging pathogens from drinking water: comparison with standard mpn method.a defined substrate method media in bacteriological water testing kit (bwtk) has been developed to recover and detect the presence/absence of total coliforms and emerging pathogens from drinking water without the need for the confirmatory or complete tests. this method is based on technology that uses a hydrolysable substrate as a specific indicator- nutrient for the target microbes. to perform the test, one only has to add water to the ingredients in the bwtk and incubate at room temperature fo ...201020706015
development and evaluation of a multiplex pcr assay for simultaneous detection of flavobacterium psychrophilum, yersinia ruckeri and aeromonas salmonicida subsp. salmonicida in culture fisheries.bacterial cold water disease, enteric red mouth disease and frunculosis are the common bacterial diseases of fish worldwide. the etiologic agents of these diseases are flavobacterium (f.) psychrophilum, yersinia (y.) ruckeri and aeromonas (a.) salmonicida subsp. salmonicida, respectively. in this study, a multiplex polymerase chain reaction (m-pcr) method with yer8/10-fer3/4-fp1/3 primer pairs which can identify these fish pathogens simultaneously was developed and optimized. in optimized condit ...201020706031
screening of antibacterial activity of mucus extract of snakehead fish, channa striatus (bloch).the objective of this study is to gain a better understanding of the antimicrobial properties of the mucus extract of snakehead fish, channa striatus against selected human and fish pathogenic microbes.201020707287
prediction of whole-genome dna g+c content within the genus aeromonas based on housekeeping gene sequences.different methods are available to determine the g+c content (e.g. thermal denaturation temperature or high performance liquid chromatography, hplc), but obtained values may differ significantly between strains, as well as between laboratories. recently, several authors have demonstrated that the genomic dna g+c content of prokaryotes can be reliably estimated from one or several protein coding gene nucleotide sequences. few g+c content values have been published for the aeromonas species descri ...201020466501
bacteriological investigation on "mauro" sold in catania.seaweeds known as "mauro" are traditionally used fresh or to prepare omelettes in sicily (italy). twenty samples sold in catania between may 2005 and september 2007 were analyzed for escherichia coli, total enterobacteria, aeromonas spp., pseudomonas spp., vibrio spp., and salmonella spp. thirty vibrio strains were examined for the presence of the virulence genes, toxr, toxrs, tl, tdh, and trh in the genomes of the isolates. total enterobacteria ranged between 2.23 and 6.85 log cfu/g, and in six ...201020467809
necrotising fasciitis in both calves caused by aeromonas caviae following aesthetic liposuction.liposuction is the most widely performed cosmetic procedure in the world, and is considered safe and without serious complications. however, necrotising fasciitis has been documented as a rare complication following abdomen and thigh liposuction. we present a case of necrotising fasciitis in a 22-year-old female who underwent cosmetic liposuction in both calves. the diagnosis of necrotising fasciitis was delayed, leading to multi-organ dysfunction and skin necrosis with consequent massive skin l ...201020471929
impact of reattaching various aeromonas salmonicida a-layer proteins on vaccine efficacy in atlantic cod (gadus morhua).atypical aeromonas salmonicida causes atypical furunculosis in a whole range of farmed fish species. the bacterium comprises a heterogeneous group differing in surface components such as the a-layer protein and o-chain polysaccharide structures. previously, the a-layer protein was shown to contribute to protective immunity as a vaccine with a. salmonicida cells with reattached a-layer protein protecting significantly better than the corresponding a-layer deficient bacteria used in the atlantic c ...201020472024
immunostimulatory activities of bacillus simplex dr-834 to carp (cyprinus carpio).a bacterial strain, designated dr-834 and producing immunostimulatory activities to carp (cyprinus carpio), was isolated from qinghai-tibetan plateau permafrost soil. cultural characteristic studies suggested that this strain belongs to the genus bacillus. the nucleotide sequence of the 16s rrna gene of strain dr-834 exhibited close similarity (99%) with the 16s rrna gene of bacillus simplex. two compounds showing potent activity were isolated from secondary metabolites of the strain through bio ...201020472068
effect of conjugated linoleic acid on cyprinus carpio var. jian regarding growth, immunity, and disease resistance to aeromonas hydrophila.the present study was performed to investigate the effect of dietary conjugated linoleic acid (cla) on the growth performance, immune parameters and disease resistance to aeromonas hydrophila of juvenile jian carp. a total of 900 juvenile jian carp (10.71 +/- 0.02 g) were randomly allocated into six groups of each three replicates, feeding isonitrogenous (35% crude protein) and isolipidic (7.8% crude fat) diets containing graded amounts of cla (0, 0.5, 0.8, 1.4, 1.7, and 2.0%) for 60 days. after ...201020473720
considerations on infectious complications using a drowned lung for transplantation.recently, the applicability of lungs from drowned victims for transplantation has been anecdotically described in literature. however, no data exist about hazards or limitations. herein, we describe a case of lung transplantation from a submersion victim and the subsequent development of an aeromonas hydrophila infection in the implanted organ. based on this case we propose standard procedures, which should be followed when considering drowned donor lungs, in order to minimize risks for infectio ...201020477998
high yield expression of an ahl-lactonase from bacillus sp. b546 in pichia pastoris and its application to reduce aeromonas hydrophila mortality in aquaculture.aeromonas hydrophila is a serious pathogen and can cause hemorrhagic septicemia in fish. to control this disease, antibiotics and chemicals are widely used which can consequently result in "superbugs" and chemical accumulation in the food chain. though vaccine against a. hydrophila is available, its use is limited due to multiple serotypes of this pathogen and problems of safety and efficacy. another problem with vaccination is the ability to apply it to small fish especially in high numbers. in ...201020492673
virulence properties and characterization of aeromonads isolated from foods of animal origin and environmental sources.aeromonas species are increasingly recognized as enteric pathogens, and they possess several virulence factors that may contribute to illness. in this work, the biochemical, enzymatic, and some virulence properties of 73 potentially pathogenic strains of aeromonas spp. isolated from food and environmental sources were investigated to compare strains from different sources and establish the possible relationships between some phenotypic characters and pathogenicity. virulence factors (hemolysin a ...201020501036
high gene expression of inflammatory markers and il-17a correlates with severity of injection site reactions of atlantic salmon vaccinated with oil-adjuvanted vaccines.two decades after the introduction of oil-based vaccines in the control of bacterial and viral diseases in farmed salmonids, the mechanisms of induced side effects manifested as intra-abdominal granulomas remain unresolved. side effects have been associated with generation of auto-antibodies and autoimmunity but the underlying profile of inflammatory and immune response has not been characterized. this study was undertaken with the aim to elucidate the inflammatory and immune mechanisms of granu ...201020507624
qnrvc-like gene located in a novel complex class 1 integron harboring the iscr1 element in an aeromonas punctata strain from an aquatic environment in shandong province, china.a qnrvc-like gene, qnrvc4, was found in a novel complex class 1 integron gene cassette array following the iscr1 element and bla(per-1) in a multidrug-resistant strain of the aquatic bacterium aeromonas punctata. the deduced qnrvc4 protein sequence shares 45% to 81% amino acid identity with quinolone resistance determinants qnrb6, qnra1, qnrs1, qnrc, qnrvc1, and qnrvc3. a ser-83 to ile amino acid substitution in gyrase a may be mainly responsible for ciprofloxacin resistance in this strain.201020516288
endophytic bacterial diversity in roots of phragmites australis in constructed beijing cuihu wetland (china).the community structure and diversity of endophytic bacteria in reed (phragmites australis) roots growing in the beijing cuihu wetland, china was investigated using the 16s rrna library technique. primers 799f and 1492r were used to amplify the specific bacterial 16s rrna fragments successfully and construct the clone library. in total, 166 individual sequences were verified by colony pcr and used to assess the diversity of endophytic bacteria in reed roots. phylogenetic analysis revealed that 7 ...201020528944
subunit and small-molecule interaction of ribonucleotide reductases via surface plasmon resonance biosensor analyses.ribonucleotide reductase (rnr) synthesizes deoxyribonucleotides for dna replication and repair and is controlled by sophisticated allosteric regulation involving differential affinity of nucleotides for regulatory sites. we have developed a robust and sensitive method for coupling biotinylated rnrs to surface plasmon resonance streptavidin biosensor chips via a 30.5 a linker. in comprehensive studies on three rnrs effector nucleotides strengthened holoenzyme interactions, whereas substrate had n ...201020534631
defensin of the zebra mussel (dreissena polymorpha): molecular structure, in vitro expression, antimicrobial activity, and potential functions.a 409 bp full length defensin gene was cloned and sequenced based on an expressed sequence tag (est) obtained from a normalized zebra mussel (dreissena polymorpha) foot cdna library developed in our laboratory. the d. polymorpha defensin (dpd) gene encoded a peptide with 76 amino acid residues. the mature dpd contains 54 amino acids with a fully functional insect defensin a domain. homologue searching against genbank database suggested that this dpd was phylogenetically close to defensins from a ...201020537393
a delta-class glutathione transferase from the chinese mitten crab eriocheir sinensis: cdna cloning, characterization and mrna expression.glutathione-s-transferases (gsts), a major superfamily of antioxidative enzymes, play an important role in protecting organisms against the toxicity of reactive oxygen species (ros). the delta-class gst cdna was cloned and sequenced by rapid amplification of cdna ends (race) from the chinese mitten crab, eriocheir sinensis. the 938 bp e. sinensis gst gene, encoding a polypeptide of 216 amino acids, showed significant similarity to homologous genes in insects. the deduced amino acid sequence of t ...201020538061
biosynthesis and mobilization of a novel polyhydroxyalkanoate containing 3-hydroxy-4-methylvalerate monomer produced by burkholderia sp. usm (jcm15050).we attempted to synthesize a polyhydroxyalkanoate (pha) containing newly reported 3-hydroxy-4-methylvalerate (3h4mv) monomer by using wild type burkholderia sp. usm (jcm15050) and its transformed strain harboring the pha synthase gene of aeromonas caviae (phac(ac)). the introduction of 3h4mv as a second monomer will improve the material properties of 3hb-based polymers. to promote the accumulation of pha containing 3h4mv monomer, isocaproic acid was provided as co-carbon source. approximately 1m ...201020541932
detection and characterization of virulence genes and integrons in aeromonas veronii isolated from catfish.the presence of virulence genes and integrons was determined in 81 strains of aeromonas veronii isolated from farm-raised catfish. polymerase chain reaction (pcr) protocols were used to determine the presence of genes for cytotoxic enterotoxin (act), aerolysin (aera), two cytotonic enterotoxins (ast, alt), lipase (lip), glycerophospholipid:cholesterol acyltransferase (gcat), serine protease (ser), dnases (exu), elastase (ahyb) and the structural gene flagellin (fla) in the template dna. oligonuc ...201020227596
comparison of phenotypical and genetic identification of aeromonas strains isolated from diseased fish.phenotypicaly identified aeromonas strains (n=119) recovered mainly from diseased fish were genetically re-identified and the concordance between the results was analysed. molecular characterization based on the gcat genus specific gene showed that only 90 (75.6%) strains belonged to the genus aeromonas. the 16s rdna-rflp method identified correctly most of the strains with the exception of a few that belonged to a. bestiarum, a. salmonicida or a. piscicola. separation of these 3 species was cor ...201020227844
leech therapy in cutaneous surgery and disease.over the ages, the use of leeches in medicine has evolved from haphazard bloodletting to a well-understood physiologic process with defined, rational applications.201020232587
metabolic engineering for microbial production of polyhydroxyalkanoates consisting of high 3-hydroxyhexanoate content by recombinant aeromonas hydrophila.polyhydroxyalkanoate synthase gene phac(ah) in aeromonas hydrophila strain 4ak4 was deleted and its function was replaced by phac1(ps) cloned from pseudomonas stutzeri strain 1317 which favors 3-hydroxyhexanoate (3hhx) and longer chain length monomers. genes fadd and fadl encoding escherichia coli acyl-coa synthase and pseudomonas putida kt2442 fatty acid transport protein, respectively, were introduced into the recombinant with new phac1(ps). accumulation of a series of novel medium-chain-lengt ...201020236821
changes in skin mucus of common carp, cyprinus carpio l., after exposure to water with a high bacterial load.water in aquaculture systems may contain a high load of microorganisms. reduction in overall bacterial tank water load improves fish health and growth parameters. in this study, the effect of an increase of overall bacterial load in tank water on carp skin mucus was assessed. intracellular and released high molecular weight glycoproteins (hmgs) of carp skin mucus were analysed for changes using histological, histochemical and biochemical techniques. increase of bacterial load did not induce obvi ...201020298445
first description of atypical furunculosis in freshwater farmed atlantic salmon, salmo salar l., in chile.we report the first isolation, identification and characterization of a group of chilean strains of atypical aeromonas salmonicida isolated from freshwater farmed atlantic salmon, salmo salar. affected fish showed superficial ulcers and pale liver with or without petechial haemorrhages. outbreaks of the disease occurred in two farms in the south of chile about 2200 km apart. five strains were isolated in pure culture and identified by serological assays and immunofluorescence tests as belonging ...201020298453
production and efficacy of an aeromonas hydrophila recombinant s-layer protein vaccine for fish.a recombinant protein for the s-layer protein of aeromonas hydrophila was produced and its ability to protect common carp cyprinus carpio l. against six virulent isolates of a. hydrophila was assessed. a group of 120 carp (30-40 g) were vaccinated intra-peritoneally with 0.1 ml of adjuvanted vaccine (30 microg protein per fish). another group of 120 carp were injected with 0.1 ml of pbs-adjuvant mixture to serve as controls. twenty fish from each group were challenged with each one of six virule ...201020307596
competitive exclusion as a mode of action of a novel bacillus cereus aquaculture biological agent.to determine the contribution of potential modes of action of a bacillus cereus aquaculture biological control agent in inhibition of the fish pathogen, aeromonas hydrophila.201020337929
emergence of ctx-m-3, tem-1 and a new plasmid-mediated mox-4 ampc in a multiresistant aeromonas caviae isolate from a patient with pneumonia.aeromonas species rarely cause pulmonary infection. we report, for what is believed to be the first time, a case of severe pneumonia in a cancer patient caused by aeromonas caviae. detailed microbiological investigation revealed that this isolate carried three beta-lactamase-encoding genes (encoding mox-4, ctx-m-3 and tem-1) conferring resistance to all beta-lactams but imipenem. the beta-lactamase with a pi of 9.0 was transferred by conjugation and associated with a 7.3 kb plasmid, as demonstra ...201020339022
diversity of microbes and potential exoelectrogenic bacteria on anode surface in microbial fuel cells.single-chamber microbial fuel cells (mfcs), inoculated with anaerobic sludge and continuously run with two kinds of organic wastewater influents, were systemically investigated. the diversity of microbes, determined by 16s rdna analysis, was analyzed on three anodes under different conditions. one anode was in a closed circuit in synthetic wastewater containing glucose. the other two anodes, in open or closed circuits, were fed effluent from an anaerobic reactor treating starch wastewater. the c ...201020339216
exea binds to peptidoglycan and forms a multimer for assembly of the type ii secretion apparatus in aeromonas hydrophila.aeromonas hydrophila uses the type ii secretion system (t2ss) to transport protein toxins across the outer membrane. the inner membrane complex exeab is required for assembly of the exed secretion channel multimer, called the secretin, into the outer membrane. a putative peptidoglycan-binding domain (pfam number pf01471) conserved in many peptidoglycan-related proteins is present in the periplasmic region of exea (p-exea). in this study, co-sedimentation analysis revealed that p-exea was able to ...201020345654
[aeromonas-induced hemolytic uremic syndrome: a case report]. 201020346450
association between environmental microbiota and indigenous bacteria found in hemolymph, extrapallial fluid and mucus of anodonta cygnea (linnaeus, 1758).bivalves filter and accumulate large numbers of microorganisms present in the harvesting water. a complete understanding of the balance between anodonta cygnea and the microbiota present in their surrounding environment remains incomplete. therefore, the aim of this study was to quantify and identify the indigenous bacteria in the biological fluids of a. cygnea collected from mira lagoon in northern portugal. the results showed vibrio metschnikovii and aeromonas sobria as the dominant groups. th ...201020349058
unmethylated cpg oligodeoxynucleotides activate head kidney leukocytes of atlantic cod, gadus morhua.bacterial dna and synthetic oligodeoxynucleotides (odns) that contain unmethylated cpg motifs are strong inducers of immune response in most mammalian organisms. the use of these synthetic cpg motifs in fish, particularly in salmonids and carp, resulted in the modulation of their immune system. however, much less is known in other species of fish such as gadoids including atlantic cod, gadus morhua. using head kidney (hk) leukocytes of cod in an in vitro study, we determined the effects of some ...201020349339
[cloning and functional analysis of the collectin gene from the grass carp ctenopharyngodon idella].the grass carp (ctenopharyngodon idella) collectin gene was cloned from mixed liver and kidney cdna library. the full length sequence of grass carp collectin was 1128 bp, contained a 5' untranslated region of 229 bp and a 3' untranslated region of 104 bp. the open reading frame of grass carp collectin was 795 bp which could code a 264 amino acids polypeptide, including a terminal codon. phylogenetic analyses showed that grass carp collectin shared the highest homology with that of zebrafish (dan ...201020353088
diagnosis of aeromonas hydrophila, mycobacterium species, and batrachochytrium dendrobatidis in an african clawed frog (xenopus laevis).here we describe diagnosis of concurrent infection with aeromonas hydrophila, mycobacterium spp., and batrachochytrium dendrobatidis in a wild female xenopus laevis captured in chile and transported to the united states. after approximately 130 d in the laboratory, the frog was presented for dysecdysis and obtundation. after euthanasia, tissues were submitted for histopathologic evaluation and pcr analysis for b. dendrobatidis and ranavirus. clinically significant gross lesions included cutaneou ...201020353698
a novel ttx-producing aeromonas isolated from the ovary of takifugu obscurus.puffer fish (takifugu obscurus) from the yangtze river of china were screened for tetrodotoxin-producing bacteria. an aeromonas strain was isolated from the ovary of the puffer fish and was shown to produce tetrodotoxin; this strain was denoted ne-1. the identity of tetrodotoxin produced by strain ne-1 was confirmed by mouse bioassay, high performance liquid chromatography coupled with mass chromatography (lc-ms), and elisa. strain ne-1 was characterized morphologically, biochemically, and by 16 ...201020362605
characterization of susceptibility and carrier status of burbot, lota lota (l.), to ihnv, ipnv, flavobacterium psychrophilum, aeromonas salmonicida and renibacterium salmoninarum.in this study, susceptibility and potential carrier status of burbot, lota lota, were assessed for five important fish pathogens. burbot demonstrated susceptibility and elevated mortality following challenge with infectious haematopoietic necrosis virus (ihnv) by immersion and to aeromonas salmonicida by intraperitoneal (i.p.) injection. ihnv persisted in fish for at least 28 days, whereas a. salmonicida was not re-isolated beyond 17 days post-challenge. in contrast, burbot appeared refractory t ...201020367740
effect of dietary supplementation of inulin and vitamin c on the growth, hematology, innate immunity, and resistance of nile tilapia (oreochromis niloticus).the in vivo activities of inulin and ascorbic acid were evaluated experimentally via using 450 nile tilapia (oreochromis niloticus) that were distributed into 3 equal groups (each of three replicates). fish of the first group served as a control and received a balanced diet free from inulin and vitamin c. the second fed on balanced diet supplemented with inulin (5 g kg(-1)), whereas, the third one received a balanced diet supplemented with vitamin c (500 mg kg(-1)). the survival and growth perfo ...201020371294
aeromonas salmonicida type i pilus system contributes to host colonization but not invasion.the host-adherence strategies employed by aeromonas salmonicida subsp, salmonicida, the etiological agent of an infectious bacteremia of salmonids, are poorly understood. in addition to the outer protein coat or s-layer, a. salmonicida has both type i and type iv pili loci. the a. salmonicida type i or fim pilus is encoded by an operon with genes for a chaperone, an usher, and 3 pilus subunits and is predicted to be similar to the pap fimbriae of uropathogenic escherichia coli, which are conside ...201020377009
atlantic cod (gadus morhua) cc chemokines: diversity and expression analysis.chemokines are a large, diverse group of small cytokines that can be classified into several families, including the cc chemokines that are characterized by two adjacent cysteines near their amino terminus. cc chemokines play a pivotal role in host defense mechanisms by inducing leukocyte chemotaxis under physiological and inflammatory conditions. analysis of cc chemokines from teleost fishes indicates that the number of cc chemokine genes and their tissue expression patterns vary largely in thi ...201020381521
design and recombinant expression of insulin-like peptide 5 precursors and the preparation of mature human insl5.insulin-like peptide 5 (insl5) is a recently identified insulin superfamily member. although it binds to and activates the g-protein coupled receptor, rxfp4, its precise biological function remains unknown. to help determine its function, significant quantities of insl5 are required. in the present work, three single-chain insl5 precursors were designed, two of which were successfully expressed in e. coli cells. the expressed precursors were solubilized from inclusion bodies, purified almost to ...201020383778
[cloning and molecular organization of the polyhydroxyalkanoic acid synthase gene (phac) of ralstonia eutropha strain b5786].class i polyhydroxyalkanoic acid (pha) synthase gene (phac) of ralstonia eutropha strain b5786 was cloned and characterized. r. eutropha b5786 features the ability to synthesize multicomponent phas with short- and medium-chain-length monomers from simple carbohydrate substrate. a correlation was made between the molecular structure of pha synthase and substrate specificity and the ability of strain-producers to accumulate phas of this or that structure. a strong similarity of pha synthase of r. ...201020391757
molecular cloning and characterization of alpha 2-macroglobulin (alpha2-m) from the haemocytes of chinese mitten crab eriocheir sinensis.alpha 2-macroglobulin (alpha2-m) is a large protein in blood and is able to inactivate a variety of proteinases in invertebrates and vertebrates. the alpha2-m gene was obtained from the chinese mitten crab, eriocheir sinensis, by the reverse transcript-polymerase chain reaction (rt-pcr) and the rapid amplification of cdna ends (race). the alpha2-m cdna of e. sinensis contained 4851 bp with an open reading frame of 4371 bp, a 70 bp 5'-untranslated region, a 410 bp 3'-untranslated region, and thre ...201020394825
species differentiation of seafood spoilage and pathogenic gram-negative bacteria by maldi-tof mass fingerprinting.species differentiation is important for the early detection and identification of pathogenic and food-spoilage microorganisms that may be present in fish and seafood products. the main 26 species of seafood spoilage and pathogenic gram-negative bacteria, including aeromonas hydrophila, acinetobacter baumanii, pseudomonas spp., and enterobacter spp. among others, were characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) of low molecular wei ...201020408567
pathogenicity of aeromonas hydrophila, klebsiella pneumoniae, and proteus mirabilis to brown tree frogs (litoria ewingii).bacterial dermatosepticemia, a systemic infectious bacterial disease of frogs, can be caused by several opportunistic gram-negative bacterial species including aeromonas hydrophila, chryseobacterium indologenes, chryseobacterium meningosepticum, citrobacter freundii, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, and serratia liquifaciens. here we determined the pathogenicity of 3 bacterial species (aeromonas hydrophila, klebsiella pneumoniae, and proteus mirabilis) associated ...201020412685
the effect of ph and chemical preservatives on the growth of bacterial isolates from some nigerian packaged fruit juices.bacterial pathogens were isolated from some nigerian packaged fruit juices. the isolates were characterized and identified as bacillus licheniformis, aeromonas hydrophila, bacillus circulans, proteus morganii, pseudomonas cepacia, bacillus alvei and pseudomonas chlororaphis. the antibiotic susceptibility profile of the seven isolates was determined and it was discovered that 65% of the microorganisms isolated were resistance to the antibiotic used while 35% were sensitive. the effect of ph, benz ...201020415148
invasive bacterial infections in neonates and young infants born outside hospital admitted to a rural hospital in kenya.bacterial sepsis is thought to be a major cause of young infant deaths in low-income countries, but there are few precise estimates of its burden or causes. we studied invasive bacterial infections (ibis) in young infants, born at home or in first-level health units ("outborn") who were admitted to a kenyan rural district hospital during an 8-year period.201020418799
the influence of surfactants on cell surface properties of aeromonas hydrophila during diesel oil biodegradation.in this study the capacity of the newly isolated environmental strain aeromonas hydrophila was evaluated. the influence of three surfactants: rhamnolipides, saponins and triton x-100 on cell surface properties of the a. hydrophila environmental strain and the biodegradation process of diesel oil was studied. the surface activities in water, a mineral salts medium and in the biological system of all considered surfactants were estimated by means of equilibrium surface tension experiments. the obt ...201020708388
a lipase with broad temperature range from an alkaliphilic gamma-proteobacterium isolated in greenland.a gamma-proteobacterium related to the genera alteromonadales and pseudomonadales, isolated from a cold and alkaline environment in greenland, has been shown to produce a lipase active between 5 degrees c and 80 degrees c, with optimal activity at 55 degrees c and ph 8. pcr-based screening of genomic dna from the isolated bacterium, followed by genome walking, resulted in two complete open reading frames, which were predicted to encode a lipase and its helper protein, a lipase foldase. the amino ...201020718291
cytotoxins of the human pathogen aeromonas hydrophila trigger, via the nlrp3 inflammasome, caspase-1 activation in macrophages.aeromonas hydrophila is a gram-negative pathogen that causes serious infectious disease in humans. a. hydrophila induces apoptosis in infected macrophages, but the host proinflammatory responses triggered by macrophage death are largely unknown. here, we demonstrate that the infection of mouse macrophages with a. hydrophila triggers the activation of caspase-1 and release of il-1β. caspase-1 activation was abrogated in macrophages deficient in nod-like receptor family, pyrin domain containing 3 ...201020722078
discovery of serum l-amino acid oxidase in the rockfish sebastes schlegeli: isolation and biochemical characterization.fish have a complex innate defense mechanism against microbial invasion. in particular, epidermal mucus and serum in fish play important roles in innate immunity and contain a variety of bioactive substances such as complements, lectins and lysozymes, involved in host defense. recently, l-amino acid oxidases (laos) with antibacterial activity were isolated from the skin and/or gill mucous secretions of rockfish, great sculpin and flounder, and were identified to be a novel type of antibacterial ...201020728563
genotyping of aeromonas hydrophila by box elements.pcr-based dna fingerprinting techniques were evaluated to genotype eight diseased, particularly normal and environmental isolates of aeromonas hydrophila. pcr-based fingerprinting method has an advantage of having repetitive sequence also called box elements that are interspersed throughout the genome in diverse bacterial species. the box-pcr fingerprinting technique was evaluated for the discrimination of different isolates of a. hydrophila. all the studied isolates have shown major banding pat ...201020734813
determination of distinctive carbohydrate signatures obtained from the aeromonas hydrophila (chemotype ii) core oligosaccharide pinpointing the presence of the 4-o-phosphorylated 5-o-linked kdo reducing end group using electrospray ionization quadrupole orthogonal time-of-flight mass spectrometry and tandem mass spectrometry.the electrospray quadrupole orthogonal time-of-flight mass spectrometric (esi-qqtof-ms) structural elucidation of the core oligosaccharide of aeromonas hydrophila (chemotype ii) lipopolysaccharide has been investigated and it was demonstrated that it contained an 4-o-phosphorylated kdo reducing end group, which was glycosylated by the remaining outer core oligosaccharide through its o-5 position. after releasing the core oligosaccharide from the native lps with acid, the phosphorylated kdo resid ...201020740521
role of hcp, a type 6 secretion system effector, of aeromonas hydrophila in modulating activation of host immune cells.recently, we reported that the type 6 secretion system (t6ss) of aeromonas hydrophila ssu plays an important role in bacterial virulence in a mouse model, and immunization of animals with the t6ss effector haemolysin co-regulated protein (hcp) protected them against lethal infections with wild-type bacteria. additionally, we showed that the mutant bacteria deleted for the vash gene within the t6ss gene cluster did not express the hcp gene, while the vask mutant could express and translocate hcp, ...201020798163
purification of a toxic cysteine protease produced by pathogenic aeromonas hydrophila isolated from rainbow trout.an extracellular lethal toxin produced by aeromonas hydrophila strain rt860715k originally isolated from diseased rainbow trout (oncorhynchus mykiss) was purified by using fast protein liquid chromatography system with hydrophobic interaction chromatography and anion exchange columns. the toxin was a cysteine protease, inhibited by l -cysteine, iodoacetic acid, n -ethylamleimide, p-chloromercuibenzene-sulfonic acid and n-α-p-tosyl-1-lysine-chloromethyl ketone (tlck), and showed maximal activity ...201020806257
immunomodulatory effect of aegle marmelos leaf extract on freshwater fish cyprinus carpio infected by bacterial pathogen aeromonas hydrophila.aquatic organisms (especially fish) require potent defense mechanisms to protect themselves against pathogen invasion and disease formation. the use of immunostimulants in fish culture can prevent the diseases through augmentation of both specific and non-specific immunity.201020818950
comparison of chromogenic biolog rainbow agar shigella/aeromonas with xylose lysine desoxycholate agar for isolation and detection of shigella spp. from foods.shigella outbreaks are widely reported throughout the world. however, it remains a challenge to isolate shigella spp. from foods by using conventional microbiological media. the main objective of this study was to determine the effectiveness of a novel chromogenic medium, rainbow agar shigella/aeromonas (rainbow agar), for the isolation and detection of shigella spp. in foods. all four shigella species, s. sonnei, s. flexneri, s. dysenteriae, and s. boydii, were studied. rainbow agar was compare ...201020819355
lead distributions and risks in new orleans following hurricanes katrina and rita.during the last four years, significant effort has been devoted to understanding the effects that hurricanes katrina and rita had on contaminant distribution and redistribution in new orleans, louisiana, usa, and the surrounding gulf coast area. elevated concentrations were found for inorganic contaminants (including as, fe, pb, and v), several organic pollutants (polycyclic aromatic hydrocarbons, pesticides, and volatiles) and high concentration of bioaerosols, particularly aeromonas and vibrio ...201020821590
immobilization of the aminopeptidase from aeromonas proteolytica on mg2+/al3+ layered double hydroxide particles.a novel biomaterial formed by the immobilization of the aminopeptidase from aeromonas proteolytica (aap) on synthetic mg2+ and al3+ ion-containing layered double hydroxide (ldh) particles was prepared. immobilization of aap on the ldh particles in a buffered, aqueous mixture is rapid such that the maximum loading capacity, 1×10(-9) moles of aap/mg ldh, is achieved in a few minutes. x-ray powder diffraction of ldh samples before and after treatment with aap indicates that the enzyme does not inte ...201020825230
matrix-assisted laser desorption ionization time-of-flight mass spectrometry: usefulness for taxonomy and epidemiology.matrix-assisted laser desorption ionization time-of-flight (maldi-tof) mass spectrometry (ms) is a powerful tool for the species and subspecies classification of a broad spectrum of bacteria, including gram-positive bacteria such as staphylococcus, streptococcus and listeria, and gram-negative bacteria such as neisseria, salmonella, aeromonas, campylobacter and helicobacter. maldi-tof ms has also been used for the rapid identification and typing of potential bioterrorism agents, including coxiel ...201020825435
yeast extract, brewer's yeast and spirulina in diets for labeo rohita fingerlings affect haemato-immunological responses and survival following aeromonas hydrophila challenge.a feeding trial was conducted for 60days to study the immunomodulatory role of three different immunostimulants yeast extract (ye), brewer's yeast (by) and spirulina (sp) in labeo rohita fingerlings. four hundred and fifty fingerlings (avg. wt 3.35±0.15g) were randomly distributed in ten treatments and fed with either of ten iso-nitrogenous and iso-caloric semi-purified diets, prepared with three incremental levels (1%, 2% and 4%) of different immunostimulants except the control. growth paramete ...201020825959
electrochemical detection of foodborne pathogen aeromonas hydrophila by dna hybridization biosensor.the paper describes an electrochemical dna biosensor used for the detection of aeromonas hydrophila. this opportunistic pathogen is recognized as an emerging foodborne hazard and is associated with a variety of virulence factors including production of cytotoxic enterotoxin aerolysin. the genosensor recognition layer was prepared using mixed self-assembled monolayer (sam) consisting of thiolated single-stranded dna probe (ssdna) and diluent molecules--mercaptoalcohol: mercaptoethanol (mce) or me ...201020829023
total and pathogenic vibrio parahaemolyticus in shrimp: fast and reliable quantification by real-time pcr.vibrio parahaemolyticus is found in aquatic environments and is the leading cause of gastroenteritis due to seafood consumption worldwide. we evaluated a quantitative real-time pcr (q-pcr) assay with hydrolysis probes, to determine whether this method could be used for the efficient counting of total, tdh and trh-positive v. parahaemolyticus in shrimps. we assessed the specificity of this assay, using 62 strains from 12 non target bacterial species of the vibrio, photobacterium, shewanella and a ...201020843573
influence of microbial interactions and eps/polysaccharide composition on nutrient removal activity in biofilms formed by strains found in wastewater treatment systems.the study of biofilm function, structure and microbial interactions might help to improve our understanding of biofilm wastewater treatment processes. however, few reports specifically address the influence of interactions within multispecies biofilms on microbial activity and biofilm composition. thus, the relationship between biofilm formation, denitrification activity, phosphorus removal and the composition of extracellular polymeric substances (eps), exopolysaccharides and the bacterial comm ...201020869860
isolation and characterization of bacteria from the gut of bombyx mori that degrade cellulose, xylan, pectin and starch and their impact on digestion.bombyx mori l. (lepidoptera: bombycidae) have been domesticated and widely used for silk production. it feeds on mulberry leaves. mulberry leaves are mainly composed of pectin, xylan, cellulose and starch. some of the digestive enzymes that degrade these carbohydrates might be produced by gut bacteria. eleven isolates were obtained from the digestive tract of b. mori, including the gram positive bacillus circulans and gram negative proteus vulgaris, klebsiella pneumoniae, escherichia coli, citro ...201020874394
altered hematological and immunological parameters in silver catfish (rhamdia quelen) following short term exposure to sublethal concentration of glyphosate.using agrichemicals to control unwanted species has become a necessary and common worldwide practice to improve crop production. although most currently used agrichemicals are considered relatively safe, continuous usage contributes for soil and water contamination and collateral toxic effects on aquatic species. few studies correlated the presence of agrichemicals on fish blood cells and natural immune system. thus, in this study, silver catfish (rhamdia quelen) were exposed to sublethal concen ...201020883798
in vitro adherence of two candidate probiotics from atlantic cod and their interference with the adhesion of two pathogenic bacteria.the potential of two candidate probiotic bacteria (gp21 and gp12), isolated from the gut of atlantic cod, to adhere to primary cultures of the epithelial cells from the different regions of the intestine and to interfere with the adhesion of two pathogens, vibrio anguillarum and aeromonas salmonicida subsp. salmonicida were investigated. the intestinal isolates showed clear preference in adhering to the cells from the different intestine segments. gp12 adhered strongly to the fore- and mid intes ...201020884135
isolation of culturable microorganisms from free-ranging bottlenose dolphins (tursiops truncatus) from the southeastern united states.reports of diseases in marine mammals are increasing worldwide, however our understanding of the microorganisms associated with marine mammals is still limited. in this study, we cultured bacteria and fungi isolated from the upper respiratory tract (blowhole), gastric fluid and anus of 180 wild bottlenose dolphins (tursiops truncatus) from two estuarine locations along the southeastern atlantic coast of the united states. a total of 339 and 491 isolates from charleston, sc (chs) and indian river ...201020888150
molecular mechanisms of quinolone resistance in clinical isolates of aeromonas caviae and aeromonas veronii bv. sobria.mutations in quinolone targets were studied together with quinolone efflux pump activation and plasmid-mediated quinolone resistance determinants in nalidixic-acid-resistant isolates of aeromonas caviae and aeromonas veronii. among 135 clinical aeromonas spp. isolated from stools of patients with gastrointestinal symptoms, 40 nalidixic acid-resistant strains belonging to a. caviae and a. veronii were selected and their susceptibility to different quinolones (ciprofloxacin, norfloxacin, ofloxacin ...201020890847
characterization and immune function of two intracellular sensors, hmtlr1 and hmnlr, in the injured cns of an invertebrate.unlike mammals, the cns of the medicinal leech can regenerate damaged neurites, thus restoring neural functions. our group recently demonstrated that the injured leech nerve cord is able to mount an immune response, which promotes the regenerative processes. this defense mechanism is microorganism-specific, suggesting that the leech cns is able to discriminate among microbial components. we report here the characterization of two receptors potentially implicated in this detection: hmtlr1 and hmn ...201020920526
epithelial cell proliferation in the developing zebrafish intestine is regulated by the wnt pathway and microbial signaling via myd88.rates of cell proliferation in the vertebrate intestinal epithelium are modulated by intrinsic signaling pathways and extrinsic cues. here, we report that epithelial cell proliferation in the developing zebrafish intestine is stimulated both by the presence of the resident microbiota and by activation of wnt signaling. we find that the response to microbial proliferation-promoting signals requires myd88 but not tnf receptor, implicating host innate immune pathways but not inflammation in the est ...201020921418
bacterial enteropathogens and risk factors associated with childhood diarrhea.to investigate the etiology of bacterial enteropathogens causing diarrhea among children; to elucidate the risk factors, sign and symptoms involved in developing of infection; and to identify the antimicrobial susceptibility pattern.201020924718
potential of the melanophore pigment response for detection of bacterial toxicity.chromatophore cells have been investigated as potential biodetectors for function-based detection of chemically and biologically toxic substances. oncorhynchus tshawytscha (chinook salmon) melanophores, a chromatophore cell type containing brown pigment, rapidly detect the salmonid pathogens aeromonas salmonicida, yersinia ruckeri, and flavobacterium psychrophilum and the human pathogen bacillus cereus.201020952639
isolation and characterization of new potential probiotic bacteria based on quorum-sensing system.this work was aimed at identifying strains which can degrade quorum-sensing (qs) molecules from fish gut, with properties suitable for use as probiotic in aquaculture.201020955192
an acute gastroenteritis outbreak of vibrio parahaemolyticus o4:k55 in nursing college, thailand.a cluster of acute gastroenteritis among nursing students was noticed on 13th september 2005. between 13th and 17th september 2005, a retrospective cohort study was then conducted to identify the most likely cause of gastroenteritis at a nursing college in bangkok, thailand. self-administered questionnaires, interviews, environmental investigations, and rectal swabs from all participants were carried out. in the investigation, 98.9% female nursing students were investigated and had completed the ...201020962724
the effects of the alkyl polycyclic aromatic hydrocarbon retene on rainbow trout (oncorhynchus mykiss) immune response.the objective of this study was to examine the immune toxicity of the alkyl polycyclic aromatic hydrocarbon (pah) retene (7-isopropyl-1-methyl phenanthrene) in rainbow trout. retene is a common alkyl pah associated with combustion of terrestrial plants or industrial effluents. rainbow trout were injected intraperitoneally with a single dose of 0, 1, or 10mg/kg of retene and sampled at 21d. within each retene treatment, co-injection of formalin-killed aeromonas salmonicida or a phosphate buffered ...201020810174
[taxonomic position of certain representatives of sulphate-reducing corrosive microbial community].sulfate-reducing bacteria and their heterotrophic satellites have been isolated from the biofilm formed on steel by sulfidogenic corrosive microbial community. bacteria were characterized according to phenotypical features and investigated by the methods of molecular-genetic analysis. in accordance with the phenotypical features the studied strain of sulfate-reducing bacteria were related to desulfovibrio genus, gram-positive strains of heterotrophic satellites were related to bacillus genus, b. ...201020812503
transcriptome and expression profiling analysis revealed changes of multiple signaling pathways involved in immunity in the large yellow croaker during aeromonas hydrophila infection.the large yellow croaker (pseudosciaena crocea) is an economically important marine fish in china suffering from severe outbreaks of infectious disease caused by marine bacteria such as aeromonas hydrophila (a. hydrophila), resulting in great economic losses. however, the mechanisms involved in the immune response of this fish to bacterial infection are not fully understood. to understand the molecular mechanisms underlying the immune response to such pathogenic bacteria, we used high-throughput ...201020858287
assembly of the type ii secretion system: identification of exea residues critical for peptidoglycan binding and secretin multimerization.aeromonas hydrophila secretes a number of protein toxins across the outer membrane via the type ii secretion system (t2ss). assembly of the secretion channel exed secretin into the outer membrane is dependent on the peptidoglycan binding domain of exea. in this study, the peptidoglycan binding domain pf01471 family members were divided into a prokaryotic group and a eukaryotic group. by comparison of their sequence conservation profiles and their representative crystal structures, we found the p ...201020971903
adhesion of pathogenic bacteria to food contact surfaces: influence of ph of culture.the adhesion of aeromonas hydrophila, escherichia coli o157:h7, salmonella enteritidis, and staphylococcus aureus to hydrophobic and hydrophilic surfaces in cultures with different phs (6, 7, and 8) was studied. the results indicated that the type of material had no effect on the attachment capacity of microorganisms, while environmental ph influenced the adhesion of a. hydrophila, e. coli, and s. aureus to both solid substrates. the attachment of s. enteritidis (p > .05) was not affected by the ...201020981289
incidence and antimicrobial resistance of enteropathogens isolated from an integrated aquaculture system.to evaluate an integrated aquaculture system, microbiological analyses of water used in this system were carried out and the incidence and antimicrobial resistance of enteropathogens were determined in the related ecosystem.201021029138
biofilm formation by aeromonas hydrophila on green-leafy vegetables: cabbage and lettuce.aeromonas hydrophila is the most well known of the six species of aeromonas, which has been linked to two groups of human diseases: septicemia and gastroenteritis. reference strain atcc 7966 and biofilm strains tub19, tub20, and tub21 were investigated for their ability to form biofilm in vitro (after 48 h on polystyrene surface) and on the surface of two green-leafy vegetables, cabbage and lettuce (after 1, 2, 4, and 24 h). attachment strength (s(r)) of these strains to the vegetable surface wa ...201021034267
bacteremia caused by aeromonas hydrophila complex in the caribbean islands of martinique and guadeloupe.aeromonas species are gram-negative bacilli of the water environment whose survival appears facilitated by warm climates. there have been no reports on aeromonas hydrophila complex (a. hydrophila, a. caviae, a. veronii) in the caribbean to date. our aim was to describe clinical and bacteriological features in patients presenting with such bacteremia in martinique and guadeloupe. during a 14-year period, we retrospectively identified 37 patients. the mean age was 55 years and in 89% of cases unde ...201021036850
differential regulation of caspase-1 activation via nlrp3/nlrc4 inflammasomes mediated by aerolysin and type iii secretion system during aeromonas veronii infection.aeromonas spp. are gram-negative bacteria that cause serious infectious disease in humans. such bacteria have been shown to induce apoptosis in infected macrophages, yet the host responses triggered by macrophage death are largely unknown. in this study, we demonstrate that the infection of mouse bone marrow-derived macrophages with aeromonas veronii biotype sobria triggers activation of caspase-1 with the ensuing release of il-1β and pyroptosis. caspase-1 activation in response to a. veronii in ...201021037094
stereology and computer assisted three-dimensional reconstruction as tools to study probiotic effects of aeromonas hydrophila on the digestive tract of germ-free artemia franciscana nauplii.validation of stereology and three-dimensional reconstruction for monitoring the probiotic effect of aeromonas hydrophila on the gut development of germ-free artemia franciscana nauplii.201021040270
urinary tract infection due to aeromonas spp., a lesser known causative bacterium. 201021045364
biosynthesis of polyhydroxyalkanoate homopolymers by pseudomonas putida.pseudomonas putida kt2442 has been a well-studied producer of medium-chain-length (mcl) polyhydroxyalkanoate (pha) copolymers containing c6 ~ c14 monomer units. a mutant was constructed from p. putida kt2442 by deleting its phag gene encoding r-3-hydroxyacyl-acp-coa transacylase and several other β-oxidation related genes including fadb, fada, fadb2x, and fadax. this mutant termed p. putida kthh03 synthesized mcl homopolymers including poly(3-hydroxyhexanoate) (phhx) and poly(3-hydroxyheptanoate ...201021046374
the effect of preparation of cebiche on the survival of enterotoxigenic escherichia coli, aeromonas hydrophila, and vibrio parahaemolyticus.cebiche is a common dish in latin america, prepared using raw fish mixed with vegetables and marinated with lime juice. the acidity of the lime juice is commonly believed to destroy bacteria and render cebiche as safe to eat. little data exist concerning rates of cebiche-associated gastroenteritis outbreaks, although these may be high given the popularity of the dish.201021050320
effect of aeromonas hydrophila on reductive dechlorination of ddts by zero-valent iron.this study presents a reductive transformation method that combines zerovalent iron (zvi) and aeromonas hydrophila hs01 with iron oxide reduction property to degrade ddt (1,1-trichloro-2,2-bis(4-chlorophenyl)ethane) under anoxic conditions. the results suggest that hs01 has weak capability in terms of reducing ddt to ddd (1,1-dichloro-2,2-bis(p-chlorophenyl)ethane) and nearly failed to reduce ddd or its transformed intermediates. the coexistence of zvi and hs01 results in a slight enhancement of ...201021062044
conventional and molecular methods to detect bacterial pathogens in mussels.to detect aeromonas spp., salmonella spp., vibrio cholerae, vibrio parahaemolyticus and vibrio vulnificus in mussels and water samples from a farming area, conventional and molecular methods were applied to enrichment cultures.201021070267
biologic responses of bacteria communities living at the mucus secretion of common carp (cyprinus carpio) after exposure to the carbon nanomaterial fullerene (c(60)).bacteria communities living in mucus secretions of common carp cyprinus carpio (cyprinidae) were exposed to the organic nanomaterial fullerene (c(60)) to evaluate its potential bactericidal effects. end points analyzed were viability, growth, reactive oxygen species (ros) concentration, and total antioxidant competence against peroxyl radicals. viability was not affected (p > 0.05), whereas growth was arrested (p < 0.05) after 3 hours of exposure to the three concentration of c(60) assayed (0.1, ...201021072630
effect of acute and chronic arsenic exposure on growth, structure and virulence of aeromonas hydrophila isolated from fish.aeromonas hydrophila being a ubiquitous bacterium is prone to arsenic exposure. the present study was designed to determine the role of arsenic on growth and virulence of a. hydrophila. exposure to arsenic (1 mg l(-1) and 2 mg l(-1)) had no effect on growth but significantly inhibited the hemolytic and cytotoxic potential of exposed bacteria. transmission electron microscopy revealed loss of membrane integrity and presence of condensed cytoplasm suggestive of acute stress in bacteria exposed to ...201021074603
bacterial tolerance to silver nanoparticles (snps): aeromonas punctata isolated from sewage environment.use of silver nanoparticles (snps) is increasing in a large number of consumer products. thus, the possible build-up of the nanoparticles in the environment is becoming a major concern. aeromonas punctata isolated from sewage showed tolerance to 200 μg/ml snps. the growth kinetics data for a. punctata treated with nanoparticles were similar to those in the absence of nanoparticles. there was a reduction in the amount of exopolysaccharides (eps) in bacterial culture supernatant after nanoparticle ...201021077112
the in vitro effect of bovine lactoferrin on the activity of organ leukocytes in rainbow trout (oncorhynchus mykiss), european eel (anguilla anguilla) and wels catfish (silurus glanis).lactoferrin (lf) is a glycoprotein found in milk, neutrophil granules, secretions and selected organs of mammals. lactoferrin exhibits antibacterial, antiviral, fungicidal, immunoregulatory and other functions. although fish are devoid of this protein and its cell receptors, lf effect on the immune mechanisms of fish has been demonstrated. the objective of this study was to investigate the effect of bovine lactoferrin, applied in vitro, on the activity of head kidney and spleen leukocytes in thr ...201021077435
different clinical characteristics between polymicrobial and monomicrobial aeromonas bacteremia--a study of 216 cases.part of aeromonas bacteremia is polymicrobial infection. however, a clinical comparison of monomicrobial and polymicrobial aeromonas bacteremia has not hitherto been reported.201021088342
simple and direct detection of aeromonas hydrophila infection in the goldfish, carassius auratus (l.), by dot blotting using specific monoclonal antibodies.a combination of eight isolates of aeromonas hydrophila was used to produce monoclonal antibodies (mabs). ten different groups of mabs specific to aeromonas were selected. the first five groups of mabs demonstrated high specificity and bound to only one or two isolates of a. hydrophila. the sixth and the seventh groups of mabs were a. hydrophila specific. they recognized seven of eight a. hydrophila isolates (ah1, 2, 3, 4, 5, 6, 8); however, the mab in the seventh group also showed cross-reactiv ...201021091724
malate dehydrogenase: a useful phylogenetic marker for the genus aeromonas.the reconstruction of correct genealogies among biological entities, the estimation of the divergence time between organisms or the study of the different events that occur along evolutionary lineages are not always based on suitable genes. for reliable results, it is necessary to look at full-length sequences of genes under stabilizing selection (neutral or purifying) and behaving as good molecular clocks. in bacteria it has been proved that the malate dehydrogenase gene (mdh) can be used to de ...201021095084
acute phase response in chinese soft-shelled turtle (trionyx sinensis) with aeromonas hydrophila infection.chinese soft-shelled turtle (trionyx sinensis) is an important culture reptile. however, little is known about its acute phase response (apr) caused by bacteria. serum amyloid a (saa) is a major acute phase protein (app). in this study, a turtle saa homologue was identified and described in reptiles. the full-length cdna of turtle saa was 554 bp and contained a 381 bp open reading frame (orf) coding for a protein of 127 aa. similar to other known saa genes, the turtle saa gene contained three ex ...201021095204
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