Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| gram-negative bacteria from the camel tick hyalomma dromedarii (ixodidae) and the chicken tick argas persicus (argasidae) and their antibiotic sensitivities. | a total of nine species of gram-negative bacteria were isolated from organs and haemolymph of the hard tick hyalomma (hyalomma) dromedarii and the soft tick argas (persicargas) persicus. four species namely serratia liquefaciens, stenotrophomonas maltophilia, klebsiella ornithinolytica and aeromonas hydrophila were isolated from h. dromedarii and five species namely rahnella aquatilis, pseudomonas fluorescens, enterobacter cloacae, chryseomonas luteola and chryseobacterium meningosepticum were i ... | 2005 | 15880998 |
| a c1q-binding 40 kda porin from aeromonas salmonicida: cloning, sequencing, role in serum susceptibility and fish immunoprotection. | a 40 kda c1q-binding outer membrane protein from aeromonas salmonicida was identified by direct-binding assay with biotinylated c1q, a subcomponent of the complement classical pathway component c1. the 40 kda porin structural gene from the a450 a. salmonicida typical strain (a+:o+) was cloned in escherichia coli and sequenced. the amino acid sequence of the 40 kda a. salmonicida porin, its ability to bind c1q in an antibody independent process, and its immunological cross-reaction with the a. hy ... | 2005 | 15885977 |
| microbiological quality changes in the intestine of hybrid tilapia (oreochromis niloticus x oreochromis aureus) in fresh and frozen storage condition. | the goal of this study was to monitor the quantitative and qualitative bacterial flora in the intestine of hybrid tilapia in fresh fish and fish kept in frozen storage conditions for 1 year. | 2005 | 15892747 |
| sex and low-level sampling stress modify the impacts of sewage effluent on the rainbow trout (oncorhynchus mykiss) immune system. | the objective of the present study was to investigate the influence of chronic exposure to municipal sewage treatment effluent at environmentally relevant concentrations on immune parameters in rainbow trout (oncorhynchus mykiss), including the assessment of potential differences in reactivity between sexually mature male and female fish. trout were exposed to 1.5 and 15% (v/v) secondary treated municipal sewage effluent for 32 weeks. fish were injected intra-peritoneally either with inactivated ... | 2005 | 15892994 |
| elucidation of the molecular structure of lipid a isolated from both a rough mutant and a wild strain of aeromonas salmonicida lipopolysaccharides using electrospray ionization quadrupole time-of-flight tandem mass spectrometry. | the chemical structure of lipid a, isolated by mild acid hydrolysis from a rough mutant and a wild strain of aeromonas salmonicida lipopolysaccharide, was investigated using electrospray ionization quadrupole time-of-flight (qqtof) hybrid tandem mass spectrometry and showed a great degree of microheterogeneity. the chemical structure of the main constituent of this heterogeneous mixture was identified as a beta-d-(1 --> 6) linked d-glucosamine disaccharide substituted by two phosphate groups, on ... | 2005 | 15912470 |
| [construction and characterization of some virulence determinants-deficient strain in aeromonas hydrophila]. | cell conjugation was carried out between the donor e. coli cg120 with pam120 (tc(r)/tn916) and the recipient aeromonas hydrophila j-1 by filter mating. 3800 positive clones were gained according to the ability of growth on lb medium with 10 g/ml tetracycline (tc) and 100 g/ml cephazolin (cfz). conjugation efficiency was 3 x 10(-5) per donor. when 16s rdna pcr amplification was performed with special primers, positive results were gained in all the 38 conjugants. to demonstrate the insertion of t ... | 2005 | 15989258 |
| detection of the apr gene in proteolytic psychrotrophic bacteria isolated from refrigerated raw milk. | bacteria of the genus pseudomonas have been associated with the spoilage of raw milk and dairy products due to the production of thermostable proteolytic enzymes. the apr gene encodes for alkaline metalloprotease in pseudomonas and other related bacteria. its presence in psychrotrophic proteolytic bacteria isolated from raw milk collected from cooling tanks was verified. a polymerase chain reaction (pcr) technique was used with degenerate primers. total dna from 112 isolates was pooled in differ ... | 2005 | 15992619 |
| development of a simple and rapid fluorogenic procedure for identification of vibrionaceae family members. | we describe a simple colony overlay procedure for peptidases (copp) for the rapid fluorogenic detection and quantification of vibrionaceae from seawater, shellfish, sewage, and clinical samples. the assay detects phosphoglucose isomerase with a lysyl aminopeptidase activity that is produced by vibrionaceae family members. overnight cultures are overlaid for 10 min with membranes containing a synthetic substrate, and the membranes are examined for fluorescent foci under uv illumination. fluoresce ... | 2005 | 16000757 |
| the long-distance tertiary air transfer and care of tsunami victims: injury pattern and microbiological and psychological aspects. | on december 26, 2004, a giant earthquake shocked southeast asia, triggering deadly flood waves (tsunami) across the indian ocean. more than 310,000 people have been reported dead and millions left destitute. shortly thereafter, european governments organized airborne home transfer of most severely injured tourists using medevac aircraft. on arrival, patients were distributed to various medical centers. one cohort of the severely injured was admitted to the cologne-merheim medical center (germany ... | 2005 | 15891349 |
| antibacterial effect of cu2+-exchanged montmorillonite on aeromonas hydrophila and discussion on its mechanism. | montmorillonite (mmt) and its cu2+-exchanged montmorillonite (cu-mmt) were used to study the antibacterial activity on aeromonas hydrophila. the results indicated that mmt had no antibacterial activity. the minimum inhibitory concentration (mic) and bactericidal concentration (mbc) of cu-mmt on a. hydrophila are found to be 150 and 600 mg/l, respectively. the continuance of the antibacterial activity of cu-mmt was much longer than copper sulfate. in order to reveal the mechanism of the antibacte ... | 2005 | 15939555 |
| attenuated virulence of an aeromonas salmonicida subsp. salmonicida type iii secretion mutant in a rainbow trout model. | aeromonas salmonicida subsp. salmonicida is the causative agent of furunculosis, a severe systemic disease affecting salmonid fish. this bacterium contains a type iii protein secretion system that is responsible for the secretion and translocation of the adp-ribosylating toxin, aext, into the cytosol of fish cells. this study showed that inactivation of the type iii secretion system by marker-replacement mutagenesis of the gene ascv, which encodes an inner-membrane component of the type iii secr ... | 2005 | 15942017 |
| use of clostridium septicum alpha toxins for isolation of various glycosylphosphatidylinositol-deficient cells. | in eukaryotic cells, various proteins are anchored to the plasma membrane through glycosylphosphatidylinositol (gpi). to study the biosynthetic pathways and modifications of gpi, various mutant cells have been isolated from the cells of chinese hamster ovaries (cho) supplemented with several exogenous genes involved in gpi biosynthesis using aerolysin, a toxin secreted from gram-negative bacterium aeromonas hydrophila. alpha toxin from gram-positive bacterium clostridium septicum is homologous t ... | 2005 | 15995645 |
| clinical evaluation of an aerolysin-based screening test for paroxysmal nocturnal haemoglobinuria. | recently, a toxin produced by aeromonas hydrophila was demonstrated to bind directly to the glycosyl-phosphatidyl-inositol (gpi) anchor. after coupling it to a fluorescent dye and applying it in fluorescence-activated cell scanning (facs), this property was exploited to detect gpi-negative cells in the diagnosis of paroxysmal nocturnal haemoglobinuria (pnh). | 2005 | 15952213 |
| spectroscopic studies on cobalt(ii)-substituted metallo-beta-lactamase imis from aeromonas veronii bv. sobria. | in an effort to probe the structure of a group bb metallo-beta-lactamase, co(ii)-substituted imis was prepared and characterized by electronic absorption, nmr, and epr spectroscopies. imis containing 1 equiv of co(ii) (co(ii)(1)-imis) was shown to be catalytically active. electronic absorption studies of co(ii)(1)-imis revealed the presence of two distinct features: (1) an intense sulfur to co(ii) ligand to metal charge transfer band and (2) less intense, co(ii) ligand field transitions that sug ... | 2005 | 15794654 |
| a pore-forming toxin produced by aeromonas sobria activates ca2+ dependent cl- secretion. | bacteria produce many types of hemolysin that induce diarrhea by mechanisms that are not completely understood. aeromonas sobria hemolysin (ash) is a major virulence factor produced by a. sobria, a human pathogen that causes diarrhea. since epithelial cells in the intestine are the primary targets of hemolysin, we investigated the effects of ash on ion transport in human colonic epithelial (caco-2) cells. ash increased short-circuit currents (isc) in a dose-dependent manner, and it also activate ... | 2005 | 15797812 |
| lack of agreement between biochemical and genetic identification of aeromonas spp. | biochemical and genetic identification by rflp (restriction fragment length polymorphism) of the pcr-amplified 16s r-rna sequence were compared for a selection of 171 clinical and environmental isolates of aeromonas spp. the investigation revealed large differences between the two methods. the species phenotypic identification scheme and the genetic technique applied to the environmental strains gave divergent results for 96% of the strains tested. there was 46% discrepancy between the two metho ... | 2005 | 15799764 |
| adaptational changes in cellular fatty acid branching and unsaturation of aeromonas species as a response to growth temperature and salinity. | the effects of growth temperature and salinity on the cellular fatty acids were investigated on aeromonas caviae, aeromonas hydrophila and aeromonas sobria. under optimal growth conditions, fatty acids patterns were dominated by even-numbered chains c(16:0), c(16:1cis9), c(18:1cis11), c(12:0) and c(14:0). growth temperature modifications induced, in the three aeromonas species, important changes in fatty acid (i) unsaturation, (ii) branching and (iii) chain length. an important decrease in the c ... | 2005 | 15925007 |
| nonbioluminescent strains of photobacterium phosphoreum produce the cell-to-cell communication signal n-(3-hydroxyoctanoyl)homoserine lactone. | bioluminescence is a common phenotype in marine bacteria, such as vibrio and photobacterium species, and can be quorum regulated by n-acylated homoserine lactones (ahls). we extracted a molecule that induced a bacterial ahl monitor (agrobacterium tumefaciens nt1 [pzlr4]) from packed cod fillets, which spoil due to growth of photobacterium phosphoreum. interestingly, ahls were produced by 13 nonbioluminescent strains of p. phosphoreum isolated from the product. of 177 strains of p. phosphoreum (i ... | 2005 | 15812045 |
| selectivity and specificity of a chromogenic medium for detecting vibrio parahaemolyticust. | the thiosulfate-citrate-bile salts-sucrose agar (tcbs) used in the most-probable-number method for detecting vibrio parahaemolyticus cannot differentiate growth of v. parahaemolyticus from vibrio vulnificus or vibrio mimicus. this study examined the selectivity and specificity of bio-chrome vibrio medium (bcvm), a chromogenic medium that detects v. parahaemolyticus on the basis of the formation of distinct purple colonies on the medium. a panel consisting of 221 strains of bacteria, including 17 ... | 2005 | 16013386 |
| evaluation of an experimental aeromonas salmonicida epidemic in chinook salmon, oncorhynchus tshawytscha (walbaum). | to determine the dynamics of the transmission of aeromonas salmonicida ssp. salmonicida infection, chinook salmon, oncorhynchus tshawytscha, were exposed to bacteria by cohabitation. the latent period (time between exposure and infectivity) was determined by exposing a group of chinook salmonid fingerlings to a. salmonicida by bath, then, at daily intervals, by holding five exposed (donor) fish with approximately 50 naive fish for 24 h. the latent period was 3 days post-infection and the time pe ... | 2005 | 15892751 |
| standardization of a broth microdilution susceptibility testing method to determine minimum inhibitory concentrations of aquatic bacteria. | a multiple laboratory study was conducted in accordance with the standards established by the clinical and laboratory standards institute (clsi), formerly the national committee for clinical laboratory standards (nccls), for the development of quality control (qc) ranges using dilution antimicrobial susceptibility testing methods for bacterial isolates from aquatic animal species. qc ranges were established for escherichia coli atcc 25922 and aeromonas salmonicida subsp. salmonicida atcc 33658 w ... | 2005 | 15997819 |
| cloning and analysis of non-specific cytotoxic cell receptor (nccrp)-1 from common carp cyprinus carpio l. | nonspecific cytotoxic cell receptor protein (nccrp-1) provides an important function in target cell recognition and activation of cytotoxicity. nccrp-1 has been cloned from common carp cyprinus carpio l. from fish barbel by est analysis. the isolated gene is composed of 945 bp with a 79 bp 5' utr, 714 bp open reading frame and 152 bp 3' utr. the predicted nccrp-1 gene is composed of 237 amino acid residues and its predicted signal peptide is 19 amino acid residues in length. this gene has conser ... | 2005 | 15897012 |
| quorum sensing signal molecules (acylated homoserine lactones) in gram-negative fish pathogenic bacteria. | the aim of the present study was to investigate the production of quorum sensing signals (specifically acylated homoserine lactones, ahls) among a selection of strains of gram-negative fish bacterial pathogens. these signals are involved in the regulation of virulence factors in some human and plant-pathogenic bacteria. a total of 59 strains, representing 9 different fish pathogenic species, were tested against 2 ahl monitor bacteria (agrobacterium tumefaciens nt1 [pzlr4] and chromobacterium vio ... | 2005 | 16042042 |
| white spot syndrome virus isolates of tiger shrimp penaeus monodon (fabricious) in india are similar to exotic isolates as revealed by polymerase chain reaction and electron microscopy. | microbiological analysis of samples collected from cases of white spot disease outbreaks in cultured shrimp in different farms located in three regions along east coast of india viz. chidambram (tamil nadu), nellore (andhra pradesh) and balasore (orissa), revealed presence of vibrio alginolyticus, vibrio parahaemolyticus, and aeromonas spp. but experimental infection trials in penaeus monodon with these isolates did not induce any acute mortality or formation of white spots on carapace. infectio ... | 2005 | 16053274 |
| comparative pathogenicity of bacteraemic isolates of aeromonas hydrophila and klebsiella pneumoniae. | two bacteraemic isolates of aeromonas hydrophila and klebsiella pneumoniae, ah-2743 and kp-129, respectively, were studied for their relative pathogenicity in comparison with escherichia coli atcc 25922. ah-2743 caused significantly higher serum levels of tumour necrosis factor-alpha, interleukin (il)-1beta or il-6 in human whole blood, and higher serum il-1beta and il-6 levels in infected mice, than did kp-129 and e. coli atcc 25922. in addition, in balb/c mice infected by intraperitoneal inocu ... | 2005 | 15966973 |
| electroporation-mediated transformation of aeromonas hydrophila. | a strain of aeromonas hydrophila producing copolyesters of 3-hydroxybutyrate and 3-hydroxyhexanoate, abbreviated as phbhhx, was successfully transformed by electroporation. the plasmid used was a broad host range plasmid pbbr1mcs. electroporation conditions were varied systemically to develop an electroporation protocol. the optimal yield of transformant was approximately 4x10(2) cfu/microg dna at 12.5 kv/cm and 1000 omega, resulting in a time constant of approximately 5 ms. the a. hydrophila tr ... | 2005 | 15967502 |
| aeromonas hydrophila-associated skin lesions and septicaemia in a nile crocodile (crocodylus niloticus). | aeromonas hydrophila is one of the most common bacteria associated with the aquatic environment. there are, however, limited data on a. hydrophila infection in crocodilians. the aim of this report is to describe a case of skin lesions and septicaemia associated with a. hydrophila in a nile crocodile (crocodylus niloticus). a captive male crocodile in the zoological park of antalya (turkey) was found dead without showing signs of any disease. gross examination showed brown or red-spotted skin les ... | 2005 | 15900900 |
| polymorphism of aeromonas spp. trna intergenic spacers. | we investigated the length polymorphism of the intergenic spacers lying between trna genes of aeromonas spp. a total of 69 strains representing all known genomic species of aeromonas were used in the study. tdna-pcr patterns were examined by dice coefficient (s(d)) and unweighted pair group method of clustering (upgma). the strains were allocated into 15 groups at a similarity level of 70%. the strains belonging to seven genomic species: a. hydrophila (hg 1), a. caviae (hg 4), a. sobria (hg 7), ... | 2005 | 15900969 |
| identification and characterization of putative virulence genes and gene clusters in aeromonas hydrophila ppd134/91. | aeromonas hydrophila is a gram-negative opportunistic pathogen of animals and humans. the pathogenesis of a. hydrophila is multifactorial. genomic subtraction and markers of genomic islands (gis) were used to identify putative virulence genes in a. hydrophila ppd134/91. two rounds of genomic subtraction led to the identification of 22 unique dna fragments encoding 19 putative virulence factors and seven new open reading frames, which are commonly present in the eight virulence strains examined. ... | 2005 | 16085838 |
| role of microfilaments and microtubules in the invasion of epc cells by aeromonas hydrophila. | the influence of the cytoskeleton on the invasion of aeromonas hydrophila strain ahj-1, isolated from diseased fish, in the monolayer cell of epithelioma papillosum cells of carp (epc) was evaluated by the recovery of gentamicin-resistant bacteria from triton x-100 cell lysates. the depolymerization of microfilaments (mf) by cytochalasin b and d inhibited the uptake of a. hydrophila in a dose-dependent manner and that of microtubules (mt) by colchicines and nocodazole did not affect the invasion ... | 2005 | 16000113 |
| molecular cloning, characterization and expression analysis of qm gene from grass carp (ctenopharyngodon idellus) homologous to wilms' tumor suppressor. | qm, a novel gene that was originally identified as a tumor suppressor, has been cloned from species encompassing members of higher vertebrate, plant and fungal kingdoms, but it is not well documented in fish. in present study, a gene homologous to qm was obtained from grass carp (ctenopharyngodon idellus) head kidney and spleen cdna library. the full-length grass carp qm (gcqm) cdna of 759 bp contains a short 5' utr of 22 bp, a 3' utr of 89 bp and an open reading frame of 648 nucleotides that tr ... | 2005 | 15936234 |
| [lung abscess caused by aeromonas hydrophila]. | 2005 | 16029738 | |
| antibiotic resistance and antibacterial activity in heterotrophic bacteria of mineral water origin. | antibiotic resistance and antibacterial activity were determined on heterotrophic bacteria isolated from mineral waters. of the 120 isolates pseudomonas spp. (55.8%) was the predominant group followed by acinetobacter spp. (14.17%), flavobacterium spp. (10.83%), achromobacter spp. (10%), burkholderia cepacia (3.3%), agrobacterium/radiobacter (2.5%), moraxella spp. (1.7%), aeromonas hydrophila (1.7%). over 80% of the isolates were resistant to one or more antibiotics and the highest resistance wa ... | 2005 | 15993695 |
| quantitative detection of legionella pneumophila in water samples by immunomagnetic purification and real-time pcr amplification of the dota gene. | a new real-time pcr assay was developed and validated in combination with an immunomagnetic separation system for the quantitative determination of legionella pneumophila in water samples. primers that amplify simultaneously an 80-bp fragment of the dota gene from l. pneumophila and a recombinant fragment including a specific sequence of the gyrb gene from aeromonas hydrophila, added as an internal positive control, were used. the specificity, limit of detection, limit of quantification, repetit ... | 2005 | 16000746 |
| [aeromonas caviae infection mimicking inflammatory bowel disease in a child]. | 2005 | 16008044 | |
| [aeromonas caviae infection mimicking inflammatory bowel disease in a child]. | 2005 | 16008045 | |
| management of extremity trauma and related infections occurring in the aquatic environment. | wounds sustained in oceans, lakes, and streams are exposed to a milieu of bacteria rarely encountered in typical land-based injuries. these include vibrio species, aeromonas hydrophila, pseudomonas and plesiomonas species, erysipelothrix rhusiopathiae, mycobacterium marinum, and other microbes. failure to recognize and treat these less common pathogens in a timely manner may result in significant morbidity or death. initial antibiotic therapy should address common gram-positive and gram-negative ... | 2005 | 16112981 |
| a study on inhibitory effects of siğla tree (liquidambar orientalis mill. var. orientalis) storax against several bacteria. | in this study, siğla (liquidambar orientalis mill.) storax (styrax) was investigated for antibacterial activity against aeromonas hydrophila, bacillus amyloliquefaciens, b. brevis, b. cereus, b. megaterium, b. subtilis, corynebacterium xerosis, enterobacter aerogenes, enterococcus faecalis, escherichia coli, e. coli o157:h7, klebsiella pneumoniae, listeria monocytogenes, micrococcus luteus, mycobacterium smegmatis, proteus vulgaris, pseudomonas aeruginosa, p. fluorescens, staphylococcus aureus a ... | 2005 | 16114094 |
| [a case of idiopathic portal hypertension complicated with sepsis caused by aeromonas hydrophila]. | a 53 year old man with idiopathic portal hypertension (iph) was admitted because of high fever and diarrhea. nineteen years before admission, he had received splenic hilar renal shunt operation with proximal flush ligation of splenic vein due to gastric varices. three months before admission, he had been admitted to our hospital for evaluation of liver dysfunction. liver biopsy examination had revealed peri-portal fibrosis consistent with iph. aeromonas hydrophila was isolated from blood. althou ... | 2005 | 16119770 |
| characterization of a galactose binding serum lectin from the indian catfish, clarias batrachus: possible involvement of fish lectins in differential recognition of pathogens. | a lectin with molecular mass around 200 kda was isolated from the serum of the indian catfish clarias batrachus. the bioactivity of this serum lectin was ca2+ and ph dependent. the lectin appeared to be specific for alpha-methyl galactose and sialoglycoproteins like porcine and bovine submaxillary mucin and could agglutinate human, rabbit, mice, rat and chicken erythrocytes. this fish lectin was able to specifically agglutinate different gram negative bacteria. when it was checked against differ ... | 2005 | 15979414 |
| metabolic engineering of aeromonas hydrophila for the enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate). | wild-type aeromonas hydrophila 4ak4 produced 35-45 wt.% poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (phbhhx) consisting of 10-15 mol% 3-hydroxyhexanoate (3hhx). to enhance phbhhx production, vgb gene encoding vitreoscilla haemoglobin or fadd gene encoding escherichia coli acyl-coa synthase was co-expressed with polyhydroxyalkanoates (pha) synthesis-related genes including phbab from wautersia eutropha and phapcj from a. hydrophila. expression of vgb increased phbhhx content from 46 to 53 wt.% ... | 2006 | 15983806 |
| the contribution of aeromonas salmonicida extracellular products to the induction of inflammation in atlantic salmon (salmo salar l.) following vaccination with oil-based vaccines. | intraperitoneal injection of atlantic salmon with oil-based vaccines often results in severe side effects. aeromonas salmonicida subspecies salmonicida, a primary antigen in the vaccines, produces extracellular products (ecps) that are included in the formulation but the role of ecps in inducing side effects is not well understood. in the present study, we evaluated the contribution of ecps to early inflammatory reactions since early events determine the outcome of inflammation. five groups of a ... | 2006 | 16018934 |
| study on peptide hydrolysis by aminopeptidases from streptomyces griseus, streptomyces septatus and aeromonas proteolytica. | we developed a spectrophotometric assay for peptide hydrolysis by aminopeptidases (aps). the assay enables the measurement of free amino acids liberated by ap-catalyzed peptide hydrolysis using 4-aminoantipyrine, phenol, peroxidase, and l-amino acid oxidase. we investigated the specificity of bacterial aps [enzymes from streptomyces griseus (sgap), streptomyces septatus (ssap), and aeromonas proteolytica (aap)] toward peptide substrates using this assay method. although these enzymes most effici ... | 2006 | 16080009 |
| aeromonas septicaemia: an uncommon complication following placement of transhepatic biliary drainage devices in europe. | 2006 | 16099547 | |
| effect of achyranthes aspera on the immunity and survival of labeo rohita infected with aeromonas hydrophila. | achyranthes aspera seed was incorporated in the diets (at 0.01%, 0.1% and 0.5%) of labeo rohita, rohu fingerlings (3.0+/-0.4 g). after 2 weeks, the fish were immunized with heat-killed aeromonas hydrophila, and after a further 2 weeks the rohu were experimentally infected with aeromonas hydrophila (atcc 49140). after 7 days blood and serum were sampled to determine superoxide anion production, bactericidal activity, lysozyme, serum protein, albumin, globulin, serum glutamate oxaloacetate transam ... | 2006 | 15961319 |
| in vitro activity of telithromycin against gram-negative bacterial pathogens. | to investigate the in vitro activity of the ketolide anti-bacterial telithromycin against a range of commensal bacteria and common aerobic gram-negative respiratory and non-respiratory pathogens. | 2006 | 15996744 |
| [cloacal and nasal bacterial flora of lepidochelys olivacea (testudines: cheloniidae) from the north pacific coast of costa rica]. | cloacal and nasal bacterial flora of lepidochelys olivacea (testudines: cheloniidae) from the north pacific coast of costa rica. the aerobic cloacal and nasal bacterial flora of 45 apparently healthy female olive ridley sea turtles (lepidochelys olivacea) was studied at nancite nesting beach, in santa rosa national park (costa rican north pacific) during july and august 2002. bacterial samples were obtained by inserting sterile swabs directly into the cloaca and the nasal cavities of the turtles ... | 2006 | 18457173 |
| a simple fluorogenic method to detect vibrio cholerae and aeromonas hydrophila in well water for areas impacted by catastrophic disasters. | the colony overlay procedure for peptidases (copp) is a simple, fluorogenic assay that can rapidly detect and quantify vibrio cholerae and aeromonas hydrophila in well water. cleavage of the substrate l-lysyl-7-amino-4-trifluoromethylcoumarin by enzymes present in vibrio and aeromonas species produces fluorescent foci on cellulose acetate membranes exposed to long-wave ultraviolet light. vibrio cholerae o1, o139, o155, and a. hydrophila were readily detected using this procedure, whereas enterob ... | 2006 | 16968933 |
| induction of vascular leakage and blood pressure lowering through kinin release by a serine proteinase from aeromonas sobria. | aeromonas sobria causes septic shock, a condition associated with high mortality. to study the mechanism of septic shock by a. sobria infection, we examined the vascular leakage (vl) activity of a. sobria serine proteinase (asp), a serine proteinase secreted by this pathogen. proteolytically active asp induced vl mainly in a bradykinin (bk) b(2) receptor-, and partially in a histamine-h(1) receptor-dependent manner in guinea pig skin. the asp vl activity peaked at 10 min to 1.8-fold of the initi ... | 2006 | 17142774 |
| detection of conjugation related type four secretion machinery in aeromonas culicicola. | background: aeromonas sp. can now be considered relatively common enteropathogens due to the increase of diseases in humans. aeromonas culicicola is a gram negative rod-shaped bacterium isolated for the first time from the mosquito mid-gut, but subsequently detected in other insects and waters also. our previous study discovered that a. culicicola harbors three plasmids, which we designated as pac3249a, pac3249b and pac3249c. we investigated and report here the existence and genetic organization ... | 2006 | 17205119 |
| influence of different concentrations of nitrogen and phosphorous on aeromonas spp. growth. | this paper evaluates the influence of nitrogen and phosphorous on the growth of aeromonas previously isolated from irrigation water. three strains respectively of three species of aeromonas (a. hydrophila, a. caviae, a. sobria) (nine strains) were individually inoculated in a microcosm added with ammonium, nitrate and phosphate using different combinations (nh4-n + no3-n and nh4-n + no3-n + po4-p) and concentrations (nh4-n: from 20 to 2500 microg l-1; no3-n: from 200 to 22000 microg l-1; po4-p: ... | 2006 | 17256017 |
| fatal case of myonecrosis and septicaemia caused by aeromonas hydrophila in finland. | a 48-y-old female developed cellulitis, myonecrosis and sepsis after a prick wound in her hand while boning freshwater fish. cultures revealed aeromonas hydrophila, a gram-negative bacillus. despite prompt care the patient died 4 d after the incident. our case shows that the occurrence of severe aeromonas infections is not limited to tropical and subtropical areas of the world. | 2006 | 17148092 |
| genetic relationships of aeromonas strains inferred from 16s rrna, gyrb and rpob gene sequences. | genetic relationships among bacterial strains belonging to the genus aeromonas were inferred from 16s rrna, gyrb and rpob gene sequences. twenty-eight type or collection strains of the recognized species or subspecies and 33 aeromonas strains isolated from human and animal specimens as well as from environmental samples were included in the study. as reported previously, the 16s rrna gene sequence is highly conserved within the genus aeromonas, having only limited resolution for this very tight ... | 2006 | 17158971 |
| enteropathogenic bacteria and enterotoxin-producing staphylococcus aureus isolated from ready-to-eat foods in khon kaen, thailand. | the objective of this study was to investigate the microbiological quality of ready-to-eat food in the municipality of khon kaen, thailand. four categories of 186 food samples were collected: (1) high heat food; (2) low heat food; (3) no heat food; and, 4) on-site prepared fruit juices and beverages. of the food samples, 145 (78%) failed to meet acceptable microbiological standards, including fruit juice and beverages (100%), no heat food (91.7%), low heat food (81.7%) and high heat food (57.9%) ... | 2006 | 17333744 |
| [prevalence of the bacteria causing diarrea in a rosario hospital, argentina]. | bacterial agents causing diarrea in patients attended in a hospital of rosario, argentina. the frequency of bacterial agents causing diarrhea can vary in patients of different areas, and through the time. in an epidemiological surveillance we studied 304 patients with diarrheal diseases looking for salmonella enterica, shigella spp, campylobacter spp, aeromonas spp, yersinia enterocolitica, and diarrheogenic escherichia coli. c. jejuni was isolated in 30 patients (9,9 %), salmonella in 18 (5,9 % ... | 2006 | 17639815 |
| 16s rdna-based analysis of dominant bacterial populations associated with early life stages of coho salmon (oncorhynchus kisutch). | in this study, we used a 16s rdna-based approach to determine bacterial populations associated with coho salmon (oncorhynchus kisutch) in its early life stages, highlighting dominant bacteria in the gastrointestinal tract during growth in freshwater. the present article is the first molecular analysis of bacterial communities of coho salmon. cultivability of the salmon gastrointestinal microbiota was estimated by comparison of direct microscopic counts (using acridine orange) with colony counts ... | 2006 | 16598631 |
| deletion of the genes encoding the type iii secretion system and cytotoxic enterotoxin alters host responses to aeromonas hydrophila infection. | in our previous study, we deleted the gene encoding aeromonas outer membrane protein b (aopb), a structural component of the type iii secretion system (t3ss) from a cytotoxic enterotoxin gene (act)-minus diarrheal isolate ssu of aeromonas hydrophila. our laboratory also molecularly characterized the cytotoxic enterotoxin (act), which is secreted by the bacterium utilizing the type ii secretion system (t2ss). the act/aopb mutant exhibited significantly reduced cytotoxicity to cultured cells (e.g. ... | 2006 | 16626931 |
| antimicrobial resistance in bacteria isolated from aquaculture sources in australia. | to carry out a preliminary assessment of the occurrence of resistance to antimicrobials in bacteria that has been isolated from a variety of aquaculture species and environments in australia. | 2006 | 16630011 |
| dna fingerprinting of vibrio cholerae and aeromonas species by pulsed-field minigel electrophoresis. | dna molecules of vibrio cholerae and aeromonas species were prepared by incubating immobilized cells for 4 and 2 h, respectively, with a nonenzymatic solution that contains chemical reagents only (ndsuplus). this method gave results as reproducible as the enzymatic one that uses proteinase k, and rendered dna molecules suitable for fingerprinting by mini-chef electrophoresis. as rapid dna separations at high electric field are achieved in mini-chef chamber with low heat evolution, dna restrictio ... | 2006 | 16721902 |
| adjuvant effect of mushroom glucan and bovine lactoferrin upon aeromonas hydrophila vaccination in catla, catla catla (hamilton). | mushroom glucan and bovine lactoferrin (lf), known for their immunostimulatory potential, were used as adjuvant in conjunction with a formalin-killed aeromonas hydrophila vaccine in catla, catla catla. in vitro antigen-specific responsiveness of catla leucocytes and protective responses against experimental challenge with homologous antigen were monitored following immunization. antigen-specific proliferation, 'macrophage activating factor' (maf) production and antibody production were significa ... | 2006 | 16768713 |
| biofilm formation in an ice cream plant. | the sites of biofilm formation in an ice cream plant were investigated by sampling both the production line and the environment. experiments were carried out twice within a 20-day period. first, stainless steel coupons were fixed to surfaces adjacent to food contact surfaces, the floor drains and the doormat. they were taken for the analysis of biofilm at three different production stages. then, biofilm forming bacteria were enumerated and also presence of listeria monocytogenes was monitored. b ... | 2006 | 16779628 |
| role of bacteria in the oviposition behaviour and larval development of stable flies. | stable flies, stomoxys calcitrans (l.), are the most important pests of cattle in the united states. however, adequate management strategies for stable flies, especially for pastured cattle, are lacking. microbial/symbiont-based approaches offer novel venues for management of insect pests and/or vector-borne human and animal pathogens. unfortunately, the fundamental knowledge of stable fly-microbial associations and their effect on stable fly biology is lacking. in this study, stable flies laid ... | 2006 | 16608496 |
| comparison of antibody responses in atlantic cod (gadus morhua l.) to aeromonas salmonicida and vibrio anguillarum. | the immune system of atlantic cod (gadus morhua l.) differ from other bony fish species in that no or only very low increases in antibody levels are detected post-immunization with vibrio salmonicida or v. anguillarum. here, we report the results from the first study on comparison of antibody responses in cod to aeromonas salmonicida and v. anguillarum. a. salmonicida appear to induce a stronger antibody response in cod compared to v. anguillarum, and more individuals immunized with a. salmonici ... | 2006 | 16616955 |
| expression of and secretion through the aeromonas salmonicida type iii secretion system. | aeromonas salmonicida subsp. salmonicida is the aetiological agent of furunculosis, a disease of farmed and wild salmonids. the type iii secretion system (ttss) is one of the primary virulence factors in a. salmonicida. using a combination of differential proteomic analysis and reverse transcriptase (rt)-pcr, it is shown that a. salmonicida a449 induces the expression of ttss proteins at 28 degrees c, but not at its more natural growth temperature of 17 degrees c. more modest increases in expres ... | 2006 | 16622045 |
| serogroups, k1 antigen, and antimicrobial resistance patterns of aeromonas spp. strains isolated from different sources in mexico. | a total of 221 strains of aeromonas species isolated in mexico from clinical (161), environmental (40), and food (20) samples were identified using the automated system biomérieux-vitek. antisera for serogroups o1 to 044 were tested using the shimada and sakazaki scheme. the k1 antigen was examined using as antiserum the o7:k1c of escherichia coli. besides, we studied the antimicrobial patterns according to vitek automicrobic system. among the 161 clinical strains 60% were identified as a. hydro ... | 2006 | 16830708 |
| decolorization of triphenylmethane, azo, and anthraquinone dyes by a newly isolated aeromonas hydrophila strain. | a broad-spectrum dye-decolorizing bacterium, strain dn322, was isolated from activated sludge of a textile printing wastewater treatment plant. the strain was characterized and identified as a member of aeromonas hydrophila based on gram staining, morphology characters, biochemical tests, and nearly complete sequence analysis of 16s rrna gene and the gyrase subunit beta gene (gyrb). strain dn322 decolorized a variety of synthetic dyes, including triphenylmethane, azo, and anthraquinone dyes. for ... | 2006 | 16622679 |
| nano- and microparticles as adjuvants in vaccine design: success and failure is related to host natural antibodies. | bovine serum albumin (bsa) and the surface a-layer protein (ap) of an atypical strain of fish bacterial pathogen aeromonas salmonicida were covalently linked with polymeric nano- and microparticles, and antigenicity of the resulted conjugates was compared in mice and goldfish. distinct albeit different levels of natural bsa and ap antibodies were present in both animal species. significant stimulation of the anti-ap antibody response in mice strikingly contrasted to unresponsiveness or even supp ... | 2006 | 16842893 |
| quantitative expression (walbaum) of immunological factors in rainbow trout, oncorhynchus mykiss (walbaum), after infection with either flavobacterium psychrophilum, aeromonas salmonicida, or infectious haematopoietic necrosis virus. | to further enhance our understanding of immunological gene expression in rainbow trout, oncorhynchus mykiss, after infection with naturally occurring pathogens, a series of probes and primers were developed for the quantification of immune factors. separate groups of specific-pathogen-free rainbow trout were infected with either flavobacterium psychrophilum, aeromonas salmonicida or infectious haematopoietic necrosis virus (ihnv). three different concentrations of each pathogen were used and sam ... | 2006 | 16635061 |
| antimicrobial activity of extracts of the lichen xanthoparmelia pokornyi and its gyrophoric and stenosporic acid constituents. | the antimicrobial activity of the diethyl ether, acetone, chloroform, petroleum ether, and ethanol extracts of the lichen xanthoparmelia pokornyi and its gyrophoric acid and stenosporic acid constituents has been screened against some foodborne bacteria and fungi. both the extracts and the acids showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, proteus vulgaris, staphylococcus aureus, streptococcus faecalis, yersinia enterocol ... | 2006 | 16869486 |
| massive mortality of common carp (cyprinus carpio carpio) in the st. lawrence river in 2001: diagnostic investigation and experimental induction of lymphocytic encephalitis. | a massive fish kill affecting exclusively common carp (cyprinus carpio carpio) in the st. lawrence river, québec, canada, during the summer of 2001 was investigated by use of laboratory diagnostic methods and by an attempt to experimentally induce the disease. the ultimate causes of mortality were opportunistic bacterial infections with aeromonas hydrophila and flavobacterium sp. secondary to immunosuppression induced by physiologic (i.e., spawning) and environmental (i.e., high temperatures and ... | 2006 | 16672577 |
| [aeromonas associated with an acute diarrhea outbreak in são bento do una, pernambuco]. | an acute diarrhea outbreak, with 2170 cases, was described during january to july, 2004, in são bento do una, pernambuco. 582 stools were examined and an enteric pathogen was recovered in 25% (145 patients). aeromonas species were the most frequent (114-19.5%) and the main isolates were aeromonas caviae (57-9.8%), aeromonas veronii biovar sobria (23-3.9%), aeromonas veronii biovar veronii (15-2.6%) and other species (19-3.2%). the other isolated enteropathogens were vibrio cholerae o1-ogawa toxi ... | 2006 | 16699653 |
| anopheles gambiae (diptera: culicidae) oviposition in response to agarose media and cultured bacterial volatiles. | anopheles gambiae s.s. giles accepted a range of agarose gels, varying from 0.5 to 8% (wt:vol), for oviposition; laid more eggs on 0.5% agarose gels than on moist filter paper or on drier agarose; and laid equal numbers of eggs on 0.5% agarose gels and distilled water. larvae hatched on agarose gel substrates and crawled onto the surface of moist agarose gels, but they tended only to burst the egg cap and remain within the egg case on drier gels. a mixture of cultured bacteria, originating from ... | 2006 | 16739407 |
| aeromonas hydrophila necrotizing fasciitis. a case report. | 2006 | 16757773 | |
| perturbations in the catfish immune responses by arsenic: organ and cell specific effects. | the present study was an attempt to elucidate the effect of non-lethal arsenic (as) exposure (1/10 lc50) on different immunologically important organs and cells of a catfish. clarias batrachus l. were exposed to arsenic trioxide for different time intervals, which resulted in significant, time-dependent changes in total head kidney and splenic leucocyte count (p<0.05) and reduction in the organosomatic indices (p<0.05) of these two important immunocompetent organs. routine histological studies r ... | 2006 | 16765097 |
| translocation of viable aeromonas salmonicida across the intestine of rainbow trout, oncorhynchus mykiss (walbaum). | the pathogenic bacterium aeromonas salmonicida is the causative agent of the destructive disease furunculosis in salmonids. horizontal transmission in salmonids has been suggested to occur via the skin, gills and/or intestine. previous reports are contradictory regarding the role of the intestine as a route of infection. the present study therefore investigates the possibility of bacterial translocation across intestinal epithelia using ussing chamber technology, in vitro. intestinal segments we ... | 2006 | 16677315 |
| di-, tri-, and tetranuclear zinc hydroxamate complexes as structural models for the inhibition of zinc hydrolases by hydroxamic acids. | attempts to produce zn analogues of the structural model complexes [m2(mu-o2cr)2(o2cr)2(mu-h2o)(tmen)2] (m = ni, co, mn; r = ch(3), c(ch3)3, cf3) by the reaction of a series of zinc carboxylates with n,n,n',n'-tetramethylethylenediamine (tmen), resulted in the mononuclear complexes [zn(oac)(2)(tmen)] (1) and [zn(crot)2(tmen)].(0.5)h2o (2) for r = ch3 and (ch)2ch3, respectively, and the dinuclear complexes [zn(2)(mu-piv)(2)(piv)(2)(mu-h2o)(tmen)2] (3) and [zn2(mu-oac(f))2(oac(f))2(mu-h2o)(tmen)2] ... | 2006 | 16711700 |
| studies on ternary metallo-beta lactamase-inhibitor complexes using electrospray ionization mass spectrometry. | metallo-beta-lactamases (mbls) are targets for medicinal chemistry as they mediate bacterial resistance to beta-lactam antibiotics. electrospray-ionization mass spectrometry (esi-ms) was used to study the inhibition by a set of mercaptocarboxylates of two representative mbls with different optimal metal stoichiometries for catalysis. bcii is a dizinc mbl (class b1), whilst the cpha mbl (class b2) exhibits highest activity with a single zinc ion in the active site. experimental parameters for the ... | 2006 | 16713713 |
| time of vaccination influences development of adhesions, growth and spinal deformities in atlantic salmon salmo salar. | in august 1998, 3000 atlantic salmon salmo salar l. parr were divided into 7 groups with 2 replicates. every 6 wk until march of the following year 1 group was vaccinated. one group was held as an unvaccinated control. the fish were transferred to seawater in may 1999, and slaughtered in february 2000. temperature, fish size and photoperiod at vaccination, and the time between vaccination and sea transfer thus varied among the groups. in all vaccinated groups, growth was reduced for 1 to 2 mo fo ... | 2006 | 16724568 |
| characterization of fe(iii)-reducing enrichment cultures and isolation of fe(iii)-reducing bacteria from the savannah river site, south carolina. | the savannah river site, south carolina (srs), has been subjected to heavy metal and radionuclide contamination. dissimilatory fe(iii)-reducing bacteria, by reducing insoluble fe(iii) to soluble fe(ii), may enhance contaminant mobility through subsurface environments. in order to investigate populations of the indigenous iron-reducing microbes from the srs, duplicate enrichment cultures were initiated using a 10% inoculum of 7 sediment/soil samples, and serial dilutions were made into wolfe's mi ... | 2006 | 16730954 |
| dietary ascorbic acid may be necessary for enhancing the immune response in siberian sturgeon (acipenser baerii), a species capable of ascorbic acid biosynthesis. | siberian sturgeon (acipenser baerii brandt) is one of few fish species capable of synthesizing ascorbic acid (aa); however, it is unknown whether dietary aa is still necessary for this species to generate an optimal non-specific immune response, as is found in most teleostean species that do not synthesize aa. sturgeon fed either 0 or 500 mg aa (aa polyphosphate) equivalent kg(-1) diets for 2 weeks were then either sham or experimentally injected with aeromonas hydrophila lipopolysaccharide (lps ... | 2006 | 16901741 |
| aeromonas sharmana sp. nov., isolated from a warm spring. | a gram-negative, facultatively anaerobic bacterial strain designated gptsa-6(t) was isolated from a water sample collected from a warm spring in assam, india. preliminary analysis of the 16s rrna gene sequence of this isolate revealed its affiliation to the family aeromonadaceae. detailed characterization using a polyphasic approach indicated that strain gptsa-6(t) is most closely related to aeromonas sobria but differs significantly from existing members of the genus aeromonas. analysis of the ... | 2006 | 16902029 |
| molecular and functional characterization of a toxr-regulated lipoprotein from a clinical isolate of aeromonas hydrophila. | human diseases caused by species of aeromonas have been classified into two major groups: septicemia and gastroenteritis. in this study, we reported the molecular and functional characterization of a new virulence factor, toxr-regulated lipoprotein, or taga, from a diarrheal isolate, ssu, of aeromonas hydrophila. the taga gene of a. hydrophila exhibited 60% identity with that of a recently identified stce gene from escherichia coli o157:h7, which encoded a protein (stce) that provided serum resi ... | 2006 | 16790746 |
| influence of food system conditions on n-acyl-l-homoserine lactones production by aeromonas spp. | eleven of 13 aeromonas strains were shown to produce ahls. results of tlc showed that n-butanoyl-l-homoserine lactone (c4-hsl) was the main ahl produced in lb medium at 30 degrees c. the influence of different carbon sources, temperature, ph values and salt concentrations on ahl production was determined in eight a. hydrophila and one a. caviae strain. additionally a quantitative study of c4-hsl production by a. hydrophila strain 519 under different conditions was performed. positive results wer ... | 2006 | 16797762 |
| culture-independent characterization of the digestive-tract microbiota of the medicinal leech reveals a tripartite symbiosis. | culture-based studies of the microbial community within the gut of the medicinal leech have typically been focused on various aeromonas species, which were believed to be the sole symbiont of the leech digestive tract. in this study, analysis of 16s rrna gene clone libraries confirmed the presence of aeromonas veronii and revealed a second symbiont, clone pw3, a novel member of the rikenellaceae, within the crop, a large compartment where ingested blood is stored prior to digestion. the diversit ... | 2006 | 16820471 |
| mechanistic studies on the mononuclear znii-containing metallo-beta-lactamase imis from aeromonas sobria. | in an effort to understand the reaction mechanism of a b2 metallo-beta-lactamase, steady-state and pre-steady-state kinetic and rapid freeze quench electron paramagnetic resonance (epr) studies were conducted on imis and its reaction with imipenem and meropenem. ph dependence studies revealed no inflection points in the ph range of 5.0-8.5, while proton inventories demonstrated at least 1 rate-limiting proton transfer. site-directed mutagenesis studies revealed that lys224 plays a catalytic role ... | 2006 | 16939225 |
| necrotizing fasciitis in rheumatic diseases. | necrotizing fasciitis is an uncommon but life-threatening complication in immunocompromised hosts. we reported four patients with rheumatic diseases complicated by necrotizing fasciitis and reviewed 14 others from literature search. most patients were on corticosteroid treatment. septic shock, disseminated intravascular coagulopathy and acute renal deterioration were common giving rise to an overall mortality rate of 27.8%. septic arthritis may also complicate the condition. statistical analysis ... | 2006 | 16830885 |
| synthesis and structure activity relationships of novel non-peptidic metallo-aminopeptidase inhibitors. | racemic derivatives of 3-amino-2-tetralone were synthesised and evaluated for their ability to inhibit metallo-aminopeptidase activities. new compounds substituted in position 2 by methyl ketone, substituted oximes or hydroxamic acids as well as heterocyclic derivatives were evaluated against representative members of zinc-dependent aminopeptidases: leucine aminopeptidase (e.c. 3.4.11.1), aminopeptidase-n (e.c. 3.4.11.2), aeromonas proteolytica aminopeptidase (e.c. 3.4.11.10), and the aminopepti ... | 2006 | 16844380 |
| putative virulence factors of the aeromonas spp. isolated from food and environment in abu dhabi, united arab emirates. | thirty randomly selected aeromonas isolates from food and the environment in abu dhabi, united arab emirates, were characterized for putative virulence determinants, such as production of cytotoxin, cytotonic toxin, and hemolysin and their capacity to adhere to and invade henle 407 cells in vitro. seventy percent of the tested isolates were cytotoxin producers, and 80% were hemolytic. cytotoxin was produced by 6 of 7 a. hydrophila strains, 6 of 13 a. caviae strains, and 6 of 7 a. veronii bv. sob ... | 2006 | 16865908 |
| antimicrobial activity of vanillin against spoilage microorganisms in stored fresh-cut mangoes. | the antimicrobial activity of vanillin against four bacteria (pantoea agglomerans, aeromonas enteropelogenes, micrococcus lylae, and sphingobacterium spiritovorun), four fungi (alternaria sp., aspergillus sp., penicillium sp., and fusarium sp.), and three unidentified yeasts isolated from spoiling fresh-cut mango slices was verified in laboratory media adjusted to ph 5.0. mics of vanillin against the fungi (12.5 to 13.3 mm), bacteria (10 to 13.3 mm), and yeasts (5.0 to 6.7 mm) indicated that all ... | 2006 | 16865911 |
| dietary immunostimulants influence specific immune response and resistance of healthy and immunocompromised asian catfish clarias batrachus to aeromonas hydrophila infection. | in order to determine the efficacy and immunoreversal effect of the 4 dietary immunomodulators, viz. lactoferrin, beta-1,3 glucan, levamisole and vitamin c, on disease resistance of a commercially important catfish, clarias batrachus, fish were fed diets supplemented with various levels of these substances in 2 subgroups, healthy and immunocompromised, during a 30 d trial. an artificial immunosuppressive state was induced by giving 3 intraperitoneal (i.p.) injections of cyclophosphamide (cyp) at ... | 2006 | 16875392 |
| aeromonas spp. isolated from oysters (crassostrea rhizophorea) from a natural oyster bed, ceará, brazil. | between april and october 2002, thirty fortnightly collections of oysters (crassostrea rhizophorea) from a natural oyster bed at the cocó river estuary in the sabiaguaba region (fortaleza, ceará, brazil) were carried out, aiming to isolate aeromonas spp. strains. oyster samples were submitted to the direct plating (dp) and the presence/absence (p/a) methods. aeromonas were identified in 15 (50%) samples analyzed by the dp method and in 13 (43%) analyzed by the p/a method. a. caviae, a. eucrenoph ... | 2006 | 16847500 |
| growth of aeromonas hydrophila in the whey cheeses myzithra, anthotyros, and manouri during storage at 4 and 12 degrees c. | the fresh whey cheeses myzithra, anthotyros, and manouri were inoculated with aeromonas hydrophila strain ntcc 8049 (type strain) or with an a. hydrophila strain isolated from food (food isolate) at levels of 3.0 to 5.0 x 10(2) cfu/g of cheese and stored at 4 or 12 degrees c. duplicate samples of cheeses were tested for levels of a. hydrophila and ph after up to 29 days of storage. at 4 degrees c, a. hydrophila grew in myzithra and anthotyros with a generation time of ca. 19 h, but no growth was ... | 2006 | 16496570 |
| immune response and disease resistance of oreochromis mossambicus to aeromonas hydrophila after exposure to hexavalent chromium. | the objective of this study was to investigate the effect of chronic exposure to sublethal concentrations of hexavalent chromium (k2cr2o7) on the immune response and disease resistance of oreochromis mossambicus (peters) to bacterial aeromonas hydrophila infection. fish (45 to 50 g) were exposed to 0.005, 0.05, 0.5, and 5 mg l(-1) [0.01, 0.1, 1, and 10% lc50, respectively] of hexavalent chromium cr (vi) for 28 d. the specific immune response was assessed by antibody response to a. hydrophila by ... | 2006 | 16610584 |
| antibiotic resistance and identification of uncommon gram-negative bacteria isolated from sputum of adult patients with cystic fibrosis. | 2006 | 16613138 | |
| clinical features and risk factors for mortality in aeromonas bacteremic adults with hematologic malignancies. | aeromonas spp. often cause infections in immunocompromised patients. to specifically understand the clinical features of aeromonas bacteremic adults with hematologic malignancies, we investigated the demographic, clinical and microbiologic characteristics of aeromonas bacteremia in this patient population. | 2006 | 16604248 |
| aeromonas veronii biovar sobria bacteraemia with septic arthritis confirmed by 16s rdna pcr in an immunocompetent adult. | an 81-year-old man developed septic arthritis and bacteraemia with aeromonas veronii biovar sobria. cultures of the joint wash-out fluid were negative; however, dna matching that of aeromonas veronii was identified by 16s rdna pcr. the patient was successfully treated with a 4 week course of ciprofloxacin. no recognized risk factors were found. | 2006 | 16434719 |
| aeromonas sobria hemolysin causes diarrhea by increasing secretion of hco3-. | aeromonas sobria hemolysin (ash) is one of the major virulence factors produced by a. sobria, a causative agent of diarrhea in humans. we investigated the effects of ash on anion transport in human colonic epithelial cells. ash increased short circuit currents across the intestinal epithelia, which were suppressed by anion channel antagonists, such as carbonic anhydrase inhibitors, and by the removal of external hco3-. iliac fluid accumulation was also inhibited by carbonic anhydrase inhibitors. ... | 2006 | 16630261 |
| identification and characterization of an aeromonas salmonicida (syn haemophilus piscium) strain that reduces selenite to elemental red selenium. | a bacterium that reduces toxic and mobile selenite to insoluble elemental selenium (se0) was isolated from a laboratory scale permeable reactive biobarrier. biochemical tests and 16s rrna gene sequence alignment identified the isolate as aeromonas salmonicida. two colony types were isolated, one more resistant to selenite than the other. both grew on agar plates containing 16 mm: selenite, although the colony diameter was reduced to 8% of controls with the small colony type and to 18% with the l ... | 2006 | 16550462 |
| innate immune responses in rainbow trout (oncorhynchus mykiss, walbaum) induced by probiotics. | carnobacterium maltaromaticum b26 and carnobacterium divergens b33, which were isolated from the intestine of healthy rainbow trout (oncorhynchus mykiss, walbaum), were selected as being potentially useful as probiotics with effectiveness against aeromonas salmonicida and yersinia ruckeri. thus, rainbow trout administered with feed supplemented with b26 or b33 dosed at >10(7) cells g(-1) feed conferred protection against challenge with virulent cultures of the pathogens. moreover, both cultures ... | 2006 | 16631379 |
| molecular requirements for the colonization of hirudo medicinalis by aeromonas veronii. | 2006 | 16623399 |