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cloning and detection of the hemolysin gene of vibrio anguillarum.a 5 kb dna fragment encoding a hemolysin was cloned from the fish pathogenic bacterium vibrio anguillarum using the cosmid vector charomid9-36. an open reading frame of the hemolysin gene (vah1) was 2253 bp and corresponded to a protein of 751 amino acid residues. the deduced amino acid sequence of the vah1 gene and the previously reported vibrio cholerae ei tor hemolysin, v. vulnificus cytolysin-hemolysin, aeromonas hydrophila ahh1 hemolysin, and a. salmonicida ash4 hemolysin showed a significa ...19968878014
thermal and biological treatments to control psychrotrophic pathogens.over the past decade, advances in egg processing technologies have permitted commercial production of ultrapasteurized liquid whole egg (lwe) products with a shelf-life of greater than 10 wk at 4 c. the inactivation and control of psychrotrophic pathogens such as listeria monocytogenes and aeromonas hydrophila in extended shelf-life lwe and conventionally pasteurized egg products is an ongoing food safety concern. this manuscript reports on the common features of these two psychrotrophic pathoge ...19968878273
use of the fluorescent dye picogreen for quantification of pcr products after agarose gel electrophoresis. 19968879566
changes in microbial populations on fresh cut spinach.the microbial populations found on fresh-cut spinach leaves that were stored in gas permeable bags at 10 degrees c for 12 days were examined and identified. the microorganisms consisted of mesophilic aerobic bacteria, psychrotrophic bacteria, pseudomonadaceae, enterobacteriaceae, micrococcaceae, lactic acid bacteria and yeasts. populations of mesophiles, psychrotrophs, pseudomonadaceae and enterobacteriaceae increased sharply during the storage period. the initial populations were 10(7), 10(6), ...19968880301
incidence and toxigenicity of aeromonas hydrophila in seafood.three selective media, oxoid aeromonas agar (oa), blood ampicillin agar (ba) and starch ampicillin agar (sa) were used to evaluate the presence of aeromonas hydrophila in 66 samples of oyster, shrimp, fish and surimi products. oyster had the highest incidence, with 50% positive, whilst no a. hydrophila was found in the surimi. of the three selective media, ba displayed the highest recovery rate of a. hydrophila from seafood. forty-eight isolates from this survey were tested for their capability ...19968880302
sparfloxacin worldwide in vitro literature: isolate data available through 1994.sparfloxacin is a piperazinyl, cyclopropyl-fluoroquinolone with broad-spectrum antibacterial activity. compared to other quinolones, sparfloxacin displays improved activity against a variety of pathogens including staphylococcus, streptococcus, enterococcus, chlamydia, mycoplasma, ureaplasma, and mycobacteria species. other susceptible organism group include haemophilus, legionella, moraxella, neisseria, aeromonas, acinetobacter, bordetella, brucella, campylobacter, gardnerella, and helicobacter ...19968882890
the cardiopulmonary, eicosanoid, and tissue microanatomic effects of fluconazole during graded bacteremia.imidazole compounds have been shown to be beneficial in systemic sepsis and inflammation. the purpose of this study was to delineate the effects of fluconazole on systemic hemodynamics and on microanatomy of the heart, lung, liver, and kidney parenchyma of swine during graded bacteremia. eighteen adult swine were studied in three groups: 1), anesthesia control; 2), septic control (aeromonas hydrophila, 10(9)/ml, infused i.v. for 4 h); 3) fluconazole (fluconazole, 30 mg/kg i.v., followed by a. hy ...19968885087
expression of aeromonas caviae bla genes in escherichia coli.an isolate of aeromonas caviae 035 carried a 55.5 kb self-transferable plasmid. transfer of the plasmid to escherichia coli k12 resulted in the expression of a tem-like beta-lactamase that was not expressed in parental a. caviae. the bla gene sequence was detectable by dna hybridization and pcr amplification of the plasmid when extracted from parental a. caviae or from e. coli k12 transconjugants. sequence analysis of the bla genes revealed in each case an 858 bp open reading frame, differing fr ...19968889718
holy water--a risk factor for hospital-acquired infection.a case of hospital-acquired infection due to acinetobacter baumanii in a burns patient after exposure to holy water is described. in order to assess the infection risk, 13 samples of holy water were cultured for bacteria, (including legionellae) and yeasts. viable bacterial counts ranged from 1 center dot 3 x 10(3)-3 center dot 8 x 10(8) cfu/l (mean 3 center dot 1 x 10(7) cfu/l). a wide range of bacterial species was isolated including pseudomonas aeruginosa, enterobacter spp. escherichia coli a ...19968904372
breast feeding may protect children against campylobacter jejuni associated diarrhoea.to investigate the bacterial etiology of infant diarrhea in karachi, pakistan, 180 rectal swab samples collected from diarrhea patients under 5 years of age and 30 samples from age- and sex-matched healthy children were analyzed. enteropathogenic organisms were isolated from 70 case samples (38.9%). the two most common etiologic agents were campylobacter jejuni (19.4%) and aeromonas hydrophilia (6.1%). salmonella, shigella, and yersina appear to have a limited incidence in this age group. no ...199612346517
detección de escherichia coli toxigénica (lt) mediante la reacción en cadena de la polimerasa [the detection of toxigenic escherichia coli (lt) by the polymerase chain reaction].in this paper it is described the detection enteroxigenic escherichia coli lt (+). this method is based on the amplification of a dna fragment of 400 pairs of bases by polymerase chain reaction (prc). the oligonucleotides were designed by the authors and the characteristic patterns were observed when the samples were submitted to an electrophoresis in an agarose gel at 2%. the pcr had positive results with the strains of escherichia coli 0:149 k; 88 (lt+) collection and with 20 strains isolated ...19969805043
metabolism of proteins and glycoproteins in tumour bearing mice treated with aeromonas l-asparaginase.l-asparaginase, isolated in our laboratory, from aeromonas had been found to be antileukaemic. in the present study, changes in the levels of proteins and glycoproteins in leukaemic mice and under treatment with aeromonas l-asparaginase have been compared. levels of protein bound hexose, fucose and sialic acid which were increased during leukaemia attained normal levels when treated with l-asparaginase. the increased blood urea level declined significantly during enzyme therapy. effects of l-asp ...19969219441
application of a colorimetric dna-dna hybridization method for identification of aeromonas genomic species, isolated from diarrheal stools.the colorimetric dna-dna hybridization method for the identification of 18 strains of aeromonas spp. isolated from human stools was used. bacterial isolates were also examined by phenotypic characteristics. on the basis of biochemical tests 13 strains were included in phenogroup a. caviae and 5 strains in a. sobria. identification to the species level was obtained by colorimetric hybridization method. dna-dna similarity values showed that isolates of a. caviae group belong to hybridization group ...19969131804
bacterial diarrhea in laos, a region where cholera was endemic.there were cholera outbreaks in lao pdr since 1993. two provinces were epidemic areas of cholera in 1993. however the area of cholera occurrence increased to 7 provinces through 1994. then a bacterial survey had done for the purpose of public health improvement. epec was markedly isolated from the hospital in vientiane. in apparent infection of salmonella and non-01 vibrio cholerae with soldiers stationed in the island in mekong river belong to vientiane were pointed out. the outbreak of diarrhe ...19969253874
survey on the distribution of vibrionaceae at the seaport areas in taiwan, 1991-1994.a monthly survey on the distribution of human-pathogenic vibrionaceae of the seawater from five principal harbors in taiwan was conducted by national quarantine service from july, 1991 to february, 1994. of the total 1,167 vibrionaceae isolates, strains of vibrio alginolyticus (449 strains) were the most frequently isolated, followed by vibrio parahaemolyticus (262) , aeromonas hydrophila (153), vibrio cholerae non-o1 (86), and vibrio vulnificus (67). none of vibrio cholerae o1 was isolated. the ...199610592802
[aeromonas hydrophila pneumonia associated with a traffic accident. report of a case].the case of a patient who was driving a car after getting drunk is presented. his car turned over and he fell into an irrigation canal, and, as a result, he suffered from an incomplete drowning syndrome. he was admitted in the intensive care unit with acute inflammatory pneumonia and a strain of aeromonas hydrophila was isolated in blood. the patient's evolution was favorable. it is the first report on a case like this in our country.19969768270
in-vitro acquisition of iron from the iron---desferrioxamine complex by aeromonas hydrophila. 199611866778
differential susceptibility of aeromonads and coliforms to cefsulodin.volumn 62, no. 6, p. 1885, abstract, line 3: "for 102 of 129 aeromonas isolates" should read "for 102 of 130 aeromonas isolates." page 1887, column 1, lines 16 and 17: "and for 13 of 21 coliform isolates" should read "and for 13 of 94 coliform isolates." [this corrects the article on p. 1885 in vol. 62.].199616535434
chronic colitis after aeromonas hydrophilia infection. 199617429262
bacterial flora of captive houbara (chlamydotis undulata), kori (ardeotis kori) and rufous-crested (eupodotis ruficrista) bustards.a survey was carried out to investigate the aerobic bacterial flora of captive houbara (chlamydotis undulata), kori (ardeotis kori) and rufous-crested (eupodotis ruficrista) bustards maintained at the national avian research center, abu dhabi, united arab emirates. none of the houbara or rufous-crested bustards sampled were bred in captivity by the center but four of the kori bustards sampled were captive-bred. faecal, cloacal, oropharyngeal and skin samples (from under the wing) from clinically ...199618645872
septicemia due to aeromonas hydrophila in a pregnant woman: a case report and review of the literature.in the late 1960s, the first isolates of aeromonas were recovered from human specimens. presently, there is sufficient evidence to suggest that the different isolates of the genus aeromonas are human pathogens. the most frequent site of infection is the digestive tract, although extraintestinal infection also occurs. in those cases involving septicemia, most infections occur in individuals with underlying diseases. this report presents the case of a pregnant woman with no underlying disease or s ...199518476052
xylanase iv, an exoxylanase of aeromonas caviae me-1 which produces xylotetraose as the only low-molecular-weight oligosaccharide from xylan.a novel xylanase (xylanase iv) which produces xylotetraose as the only low-molecular-weight oligosaccharide from oat spelt xylan was isolated from the culture medium of aeromonas caviae me-1. by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the xylanase iv molecular weight was 41,000. xylanase iv catalyzed the hydrolysis of oat spelt xylan, producing exclusively xylotetraose. the acid hydrolysate of the product gave d-xylose. the enzyme did not hydrolyze either p-nitrophenyl-(beta)- ...199516535010
survival and activity of lux-marked aeromonas salmonicida in seawater.the fish pathogen aeromonas salmonicida was chromosomally marked with genes encoding bacterial luciferase, luxab, isolated from vibrio fischeri, resulting in constitutive luciferase production. during exponential growth in liquid batch culture, luminescence was directly proportional to biomass concentration, and luminometry provided a lower detection limit of approximately 10(sup3) cells ml(sup-1), 1 order of magnitude more sensitive than enzyme-linked immunosorbent assay detection. in sterile s ...199516535133
distribution of aeromonas species in the intestinal tracts of river fish.aeromonas isolates were obtained from fish intestines, water, and sediments from an urban river and identified by the dna-dna microplate hybridization method. the isolates were aeromonas veronii (22%), aeromonas caviae (18%), aeromonas hydrophila (13%), aeromonas sobria (8%), aeromonas jandaei (7%), and other aeromonas spp. (33%). aeromonas species occurred at high densities with high incidences, regardless of season. the results strongly suggest that aeromonads are indigenous in fish intestines ...199516535171
comparison of the growth and survival of larval turbot in the absence of culturable bacteria with those in the presence of vibrio anguillarum, vibrio alginolyticus, or a marine aeromonas sp.larval turbot (scophthalmus maximus) were reared on rotifers (brachionus plicatilis) in the absence of culturable bacteria for up to 14 days and exhibited growth and high rates of survival (>55% in five experiments). low numbers of known bacteria were introduced into similar cultures by exposure of the rotifers to a suspension of bacteria prior to addition of rotifers to the larval cultures; vibrio anguillarum 91079 caused a highly significant decrease (p <0.01) in the proportion of survivors in ...199516535196
[drinking water: use, consume and biological hazard].the authors drew the attention to the rising danger of water crisis also in countries usually immune from this problem. the most important causes of these crisis are presented along with some of the most important laws concerning civil use of water. the authors underline the biological risk induced by new aetiologycal agents (i.e. aeromonas hydrophyla, criptococcus, escherichia coli (o:127 h7) found in drinking water too. some of the health problems correlated to the civil use of water are also ...199511623483
[bacteria of the genus aeromonas as the causative agents of saprophytic infection].the bacteriological study of feces from 458 patients with acute enteric diseases revealed that in 3.2% of cases (in summer in 8.1% of cases) the disease was caused by aeromonas. in aeromonas strains isolated from river water in the volga delta, from fish and from raw meat the same pathogenicity factors occurred as in strains isolated from patients (hemolysin, dna-ase, protease, lecithinase, amylase, adhesins, capacity of binding congo red).19959381884
[phenotype markers in strains of aeromonas isolated in cuba from children with an acute diarrheic disease].twenty-seven cases of aeromonas isolated from 300 five-years children with acute diarrheal disease (eda) were studied, with the aim for demonstrating the occurrence of some phenotypical markers associated with enteropathogenicity; among them were lysine decarboxylation, acetil methyl carbinol production, enterotoxigenicity, cytotoxicity and hemolysis. the percentage of analysed strains had two or more of the investigated markers, and 13 (48.1%) lysed the rabbit erythrocytes with titres higher th ...19959805080
[new species of aeromonas isolated in cuba].one hundred and fifty-five strains of aeromonas isolated in the stools of children under 5 years presenting with acute diarrheal disease were studied. using the aerokey ii system for the identification of species, 47 strains were identified as aeromonas caviae, 58 as aeromonas hydrophila, 23 as aeromonas veronii biovar sobria, 14 as aeromonas trota, 9 as aeromonas veronii biovar veronii, 2 as aeromonas jandaei and 2 as aeromonas shubertii, emphasis is placed on the advantages of this method whic ...19959813480
aeromonas and plesiomonas species as bacterial agents of diarrhoea in urban and rural areas of nigeria: antibiogram of isolates.two thousand four hundred stool samples comprising 1 200 each from diarrhoeal and non-diarrhoeal (control) cases with 600 of each category from urban and rural areas were screened for the prevalence of aeromonas and plesiomonas species in the different groups. thirty (5 pc) and (14,8 pc) of aeromonas species and 14 (2,3 pc) and 46 (7,7 pc) of plesiomonas shigelloides were isolated from urban and rural areas respectively for diarrhoea cases. only eight (1,3 pc) and 18 (3 pc) of aeromonas spp. fro ...19958907605
alternative enrichment techniques for yersinia enterocolitica. 19958833820
a comparative study of the bbl crystal enteric/nonfermenter identification system and the biomerieux api20e and api20ne identification systems after overnight incubation.the bbl crystal enteric/nonfermenter (crystal, becton dickinson microbiology systems) is a new multi-test identification system for gram negative rods requiring no oil overlay or addition of reagents. one hundred and three selected gram negative rod isolates from routine clinical specimens were tested in parallel using the appropriate api20e or api20ne (biomerieux) reading after overnight incubation. the isolates included in the study, and the number tested, were as follows: acinetobacter sp, 8; ...19958771156
[serogroups, virulence and hemolytic activity of aeromon as hydrophila which caused fish bacterial septicaemia].a new infected disease called bacterial septicaemia occurred in cyprinid fish throughout china during 1989-1992. a. hydrophila was known as the most important pathogen of this harmful disease. thirty-three strains of a. hydrophila, most isolated from moribund fishes, were serogrouped by tube-agglutination with rabbit antisera. most of the isolates could be grouped into two serotypes, tps-30 and pbjs-76. these two groups could be found not only in zhejiang province but in other province in south ...19958745552
emerging pathogens: aeromonas spp.aeromonas spp. are gram-negative rods of the family vibrionaceae. they are normal water inhabitants and are part of the regular flora of poiquilotherm and homeotherm animals. they can be isolated from many foodstuffs (green vegetables, raw milk, ice cream, meat and seafood). mesophilic aeromonas spp. have been classified following the aerokey ii system (altwegg et al., 1990; carnahan et al., 1991). the major human diseases caused by aeromonas spp. can be classified in two major groups: septicemi ...19958750664
growth/survival of psychrotrophic pathogens on meat packaged under modified atmospheres. 19958750668
bacteria in surface infections of neonates.a bacteriological work on surface infections was done among live births (study group i) and neonates admitted in hospital (study group ii). out of 134 cases of conjunctivitis in group i gram-negative bacilli predominated (48.5%) with escherichia coli accounting for 29 (14.9%) cases, klebsiella species 15 (11.2%) cases, citrobacter freundii 3 (2.2%) cases, pseudomons aeruginosa 18 (13.4%) cases and aeromonas hydrophila 3 (2.2%) amongst pure isolates (73.9%). gonococcus was noted in 2 (1.5%) cases ...19958699037
[clinical and laboratory significance of inducible beta-lactamases].many species of bacteria have inducible expression of beta-lactamase and the enzyme production in these bacteria is normally held at a low level by a repressor mechanism, but in the presence of a beta-lactamase inducer this repression is lifted and enzyme production is greatly increased. inducible synthesis of beta-lactamase was first described for the gram-positive organism bacillus licheniformis. after that inducible expression of beta-lactamase was discovered in gram-negative bacteria like en ...19958643019
[various new pathogens in pneumonia].the table summarizes the many newly recognized agents causing lower respiratory tract infections: gram + cocci, streptococcus agalactiae, enterococci; gram-rods, acinetobacter, aeromonas hydrophila, eikenella corrodens; mycobacterium tuberculosis resistant to multiple drugs, intracellular organisms and viruses. some details are given in four chapters: pneumococci resistant to penicillin g which must be detected and impose new therapeutic strategies; chlamydia pneumoniae with its difficulties for ...19958525335
metal content and conformation of the metalloprotease from the marine sponge spheciospongia vesparia.we have recently purified a protease from the marine sponge spheciospongia vesparia. it consists of a single nonglycosylated polypeptide chain with a molecular weight of 29 600 and has one free thiol group. metal analysis revealed the presence of zinc at 2.02 +/- 0.05 g-atoms per mole of protein, as measured by atomic absorption spectroscopy. the circular dichroism spectrum in the far uv region (183-259 nm) indicates that the sponge protease contains appreciable amounts of beta sheet. this enzym ...19958528145
sequence analysis of two chromosomally mediated inducible beta-lactamases from aeromonas sobria, strain 163a, one a class d penicillinase, the other an ampc cephalosporinase.two beta-lactamase genes from aeromonas sobria, strain 163a, have been cloned and sequenced, one encoding a typical class c cephalosporinase, designated ceps, the other a class d penicillinase, designated amps. ceps is predicted to be a mature protein of 38 kda with a pi value of 7.0. the amino acid sequence of ceps is most similar to that of ampc from pseudomonas aeruginosa (54.7%). amps is predicted to be a mature protein of 27 kda with a pi value of 7.9 that mostly closely resembles blad from ...19958537283
role of aeromonas isolated from feces of finnish patients.aeromonas species were isolated from 249/13,027 (1.9%) stool samples submitted to the dept. of bacteriology and immunology, university of helsinki, during 1 year, to be cultured for bacterial enteropathogens. aeromonas was the third most common enteropathogen isolated, after campylobacter (3.6%) and salmonella (3.3%). isolates and clinical information from 234 aeromonas patients were available for further study. a. caviae (41%), a. hydrophila (27%), and a. veronii biovar sobria (22%) were the mo ...19958539542
aeromonas hydrophila lung abscess in a previously healthy man.we present a case of multilocular lung abscess caused by aeromonas hydrophila, found in both sputum and bronchial washing. no aspiration or other significantly contributory medical conditions were found. we believe this to be the first report of lung abscess caused by this organism in a previously healthy individual.19958539557
aeromonas sobria infection with severe muscle degeneration in a patient with alcoholic liver cirrhosis.a 56-yr-old male who had been followed for alcoholic liver disease was admitted for abdominal pain and a high fever. gastrointestinal endoscopy revealed bleeding esophageal varices that were treated by endoscopic sclerotherapy. blood culture on admission was positive for aeromonas sobria. then skin bullas and ulcers and severe muscle degeneration developed. the patient died despite extensive treatment with antibiotics. a. sobria infection in patients with liver cirrhosis is rare.19958540527
purification and some properties of six chitinases from aeromonas sp. no. 10s-24.six chitinases were purified from a culture supernatant of aeromonas sp. no. 10s-24 by ammonium sulfate precipitation, deae-sephadex a-50, butyl-toyopearl 650m, and chromatofocusing. these enzymes were most active at ph 3.5-4.5 and the optimum temperature were 50 degrees c. the molecular weights of the enzymes were 89,000 to 120,000 from sds-polyacrylamide gel electrophoresis. n-terminal amino acid sequences of the enzymes were similar to that of chitinase i.19958541660
development of a specific biotinylated dna probe for the detection of renibacterium salmoninarum.a specific dna probe for the identification of renibacterium salmoninarum, the causative agent of bacterial kidney disease (bkd), was developed from one of 3 clones prs47, prs49, and prs26 of 5.1 kb, 5.3 kb, and 11.3 kb, respectively. the biotinylated prs47/bamhi insert probe was tested on 3 dilutions of dna extracted from 3 strains of r. salmoninarum and from 1 strain each of arthrobacter protophormiae, aeromonas salmonicida, corynebacterium aquaticum, carnobacterium piscicola, listonella angui ...19958548693
presence of aeromonas trota in aquatic environment. 19958551948
treatment of aeromonas hydrophila infection in a deep tissue wound.we report the case of a young woman who sustained a knee laceration in an automobile accident and was subsequently exposed to water. although properly cleansed and closed, her wound became infected and she was initially treated with a single-dose parenteral cephalosporin. the infection progressed and cultures indicated a polymicrobial infection which included aeromonas hydrophila. significant tissue damage ensued and a lengthy hospitalization was required. aeromonas, although rarely a cause of i ...19958553050
fate of aeromonas and yersinia on modified-atmosphere-packaged (map) cod and trout.the growth/survival of aeromonas spp. and yersinia enterocolitica on cod and trout stored in two modified atmospheres at 0 degrees, 5 degrees and 12 degrees c was compared with that on aerobically stored fish. both organisms grew on aerobically stored fish at all three temperatures. growth on the modified-atmosphere-stored fish was never greater and was generally markedly less than on aerobically stored fish. this reduction in growth was greater in the higher carbon dioxide-containing atmosphere ...19958554761
tolerance to acid in ph 5.0-grown organisms of potentially pathogenic gram-negative bacteria.a wide range of potentially pathogenic species of gram-negative bacteria were far more resistant to extreme acidity (ph 2.0-3.5) when cultured at ph 5.0 (habituated to acid) than after ph 7.0 culture. the differences were particularly great for citrobacter spp., enterobacter spp., klebsiella spp. and for vibrio parahaemolyticus; substantial habituation was also observed for proteus mirabilis and aeromonas formicans but the effect was less marked for serratia marcescens and acinetobacter calcoace ...19958554762
[soft-tissue infection caused by aeromonas hydrophila].two cases of soft tissue infection by aeromonas hydrophila are presented with review of the literature.19958555306
adhesion of aeromonas sp. to cell lines used as models for intestinal adhesion.adhesion to hep-2 cells has been shown to correlate with enteropathogenicity for aeromonas species. such adhesion is thought to reflect the ability of strains to adhere to human intestinal enterocytes, although hep-2 cells are not of intestinal origin. in this study strains of aeromonas veronii biotype sobria isolated from various sources were investigated in parallel assays for their ability to adhere to hep-2 cells and to an intestinal cell line (caco-2). quantitative assays showed identical a ...19958557078
purification and characterization of a cho cell-elongating toxin produced by aeromonas hydrophila.a chinese hamster ovary (cho) cell-elongating toxin produced by aeromonas hydrophila was purified from cell-free supernatant fluids by ammonium sulfate precipitation and fast protein liquid chromatography. the purified toxin had an isolelectric point (pl) of 3.7 and a molecular weight of 70,000 in a single band on isoelectric focusing (ief) gels and sds-page gels, respectively. the n-terminal sequence, amino acids 1-20, and the amino acid content were determined from western blots of the 70 kda ...19958559035
binding specificity for four monoclonal antibodies recognizing terminal gal alpha 1-->4gal residues in haemophilus influenzae lipopolysaccharide.four murine monoclonal antibodies (mabs) reactive with the outer-core region of the lipopolysaccharide (lps) from haemophilus influenzae were generated after immunization with azide-killed h. influenzae rm.7004 ah1-2 and their epitope specificities studied. the monoclonal antibodies: mahi 6 (igm), mahi 5 (igg2a), mahi 8 (igg3), and mahi 11 (igg2b) bound to synthetic glycoconjugates or glycolipids with terminal galabiosyl (gal alpha 1-->4gal beta 1-) or globotriaosyl (gal alpha 1-->4gal beta 1 1- ...19958559043
structural organization of a bacillus subtilis operon encoding menaquinone biosynthetic enzymes.menaquinone (mk) is a non-protein component of the bacillus subtilis (bs) electron transport chain synthesized from chorismate through a series of mk-specific reactions. the genes encoding biosynthesis of the naphthoquinone ring of mk are clustered at 273 degrees on the bs chromosome. a 3.9-kb region capable of rescuing men mutants blocked in the early stages of mk biosynthesis was sequenced and found to contain three major open reading frames (orfs). the first orf (menf) has a predicted size of ...19958566759
relationship between enterotoxicity and multiple drug resistance in aeromonas spp.one hundred forty-seven isolates of aeromonas spp., including 54 from diarrhoeal patients and 93 from the environmental sources, were examined for drug sensitivity and enterotoxin production. one hundred and fifteen (78%) isolates that included all a. hydrophila, 60% of a. sobria and 89% of a. caviae showed resistance to one or more antibiotic(s). most (65%) of the resistant isolates caused fluid accumulation in rabbit ileal loops, of these 57% were a. hydrophila, 69% of a. sobria and 65% a. cav ...19958568193
abundance of aeromonas spp. in river and lake waters in and around dhaka, bangladesh.aeromonas spp. are widely distributed in the aquatic environment. in bangladesh, seasonal distribution of these organisms in aquatic environment was studied during july 1986-august 1987. water, sediment, plant, and plankton samples were collected monthly from the dhanmondi lake and the buriganga river during the study period. aeromonas spp. were isolated from all samples throughout the year. the seasonal peak of aeromonas spp. was observed in warmer months (april-may) in the surface waters. in t ...19958568196
studies on bacteriocinogenic lactobacillus isolates from selected nigerian fermented foods.ten bacteriocin-producing (bacteriocinogenic) lactobacillus isolates obtained from three nigerian fermented foods namely: kenkey, ogi and wara were tested against the following indicator organisms: lactobacillus plantarum and food borne pathogens comprising enterotoxigenic escherichia coli, enterohaemorrhagic escherichia coli, serratia, pseudomonas, vibrio cholerae, aeromonas sobria, aeromonas cavice, salmonella typhimurium, plesiomonas shigelloides and staphylococcus aureus. all the bacteriocin ...19958568643
class d and e tetracycline resistance determinants in gram-negative bacteria from catfish ponds.dna probes were used to examine tetracycline-resistant gram-negative bacteria (281 strains representing eight species) from catfish ponds. the isolates, which did not previously hybridize with the tet a, b and c determinants, were examined for the presence of tetracycline-resistance tet d and tet e determinants. the distribution of the tet d determinant ranged from 0 to 83%, depending on the bacterial species. tet e was found only in 69% of aeromonas hydrophila isolates. eighteen per cent failed ...19958569770
improved glutaminate-starch-penicillin agar for the isolation and enumeration of aeromonas hydrophila from seawater by membrane filtration.glutaminate-starch-penicillin agar (gsp) in its original formula was found unsatisfactory for the detection and the enumeration of aeromonas hydrophila from seawater by membrane filtration. it was also the case when gsp agar was adjusted to ph 8 or when ampicillin (10 mg/l) was incorporated to the original formula. the elevation of ph to 8 together with the addition of 10 mg/l ampicillin gave to the medium a high selectivity and permitted the detection of a. hydrophila from seawater. the numbers ...19958570268
isolation, and virulence profiles, of aeromonas hydrophila implicated in an outbreak of food poisoning in sweden.a case of food poisoning outbreak involving aeromonas hydrophila is reported in this study. a group of 27 people consumed a typical swedish food "landgång" which is a type of smörgåsbord containing shrimps with mayonnaise, liver paté, ham, sausage, and legume salad which was purchased from a food store. twenty-two of the 27 persons became ill within 20-34 hr of consumption of the food and reported the symptoms ranging from severe acute diarrhea, abdominal pain, headache, fever and vomiting. one ...19958577278
an alternative bacteriological medium for the isolation of aeromonas spp.two solid bacteriologic media were compared for cultivating aeromonas spp. from piscine sources: the rimler-shotts (rs) medium and a starch-glutamate-ampicillin-penicillin-based medium (sgap-10c) used for the recovery of aeromonas spp. from water samples. the selective and differential capacities of the media were assessed march through october 1992 by recovery rate and phenotype of 99 isolates representing 15 genera of bacteria. recovery frequency of aeromonas spp. (n = 62) was similar at 97% o ...19958583652
cloning and sequence analysis of vibrio parahaemolyticus ompk gene encoding a 26-kda outer membrane protein, ompk, that serves as receptor for a broad-host-range vibriophage, kvp40.the ompk gene of vibrio parahaemolyticus 1010 (rimd 2210001) encoding an outer membrane protein (omp), ompk, which serves as the receptor for a broad-host-range vibriophage, kvp40, was cloned and sequenced. the gene consisted of 789 nucleotides encoding 263 amino acids. since the first 20 amino acids most likely constitute the signal peptide, mature ompk would consist of 243 amino acids with a calculated molecular mass of 27458 da. sequence comparisons indicate that ompk is unique among vibrio o ...19958586275
characterization of aeromonas trota strains that cross-react with vibrio cholerae o139 bengal.it has previously been shown that vibrio cholerae o139 bengal shares antigens with v. cholerae serogroups o22 and o155. we detected six surface water isolates of aeromonas trota that agglutinated in polyclonal antisera to v. cholerae o139 and v. cholerae o22 but not in antiserum to v. cholerae o155. on the basis of agglutinin-absorption studies, the antigenic relationship between the cross-reacting bacteria were found to be in an a,b-a,c fashion, where a is the common antigenic epitope and b and ...19958586685
study of the humoral response of atlantic salmon (salmo salar l.), naturally infected with aeromonas salmonicida ssp. achromogenes.the humoral antibody response of healthy atlantic salmon and of two groups of salmon, naturally infected with aeromonas salmonicida ssp. achromogenes, was examined in some detail. one diseased group was chronically infected and the other recently infected. it was found that the humoral response of these two infected groups was quite different. the chronically infected fish showed poor specific response to the causative agent whereas the recently infected salmon produced strong specific antibody ...19958588335
chronic diarrhea among adults in kigali, rwanda: association with bacterial enteropathogens, rectocolonic inflammation, and human immunodeficiency virus infection.one hundred patients with chronic diarrhea were seen in the department of internal medicine at the centre hospitalier de kigali, rwanda; stool and/or rectal swab culture was performed for these patients, and they underwent rectoscopy and serological testing for human immunodeficiency virus type 1 (hiv-1). enteropathogenic bacteria were isolated from 39 (39%) of the patients: shigella species (22 of 100 patients tested), non-typhi salmonella (11/100), aeromonas species (5/60), and campylobacter s ...19958589155
attachment of non-culturable toxigenic vibrio cholerae o1 and non-o1 and aeromonas spp. to the aquatic arthropod gerris spinolae and plants in the river ganga, varanasi.non-cultivable, pathogenic o1 and non-o1 vibrio cholerae and aeromonas spp. were resuscitated from aquatic arthropods and plant homogenate respectively, by rabbit ileal loop (ril) assay. these organisms adhered to the aquatic arthropod gerris spinolae and various species of phytoplankton in the river ganga, but failed to grow after direct inoculation on artificial media except for only 10 homogenates of the arthropod. the number of non-o1 v. cholerae and aeromonas recovered on direct inoculation ...19958589660
capsulated cells of aeromonas salmonicida grown in vitro have different functional properties than capsulated cells grown in vivo.when grown in vivo in the peritoneal cavity of rainbow trout, aeromonas salmonicida produces a clearly defined capsule with virulence-related functions. aeromonas salmonicida grown in vitro in a glucose-rich medium (grm) has also been reported to reproduce capsular material. because in vitro mimicry of in vivo induced traits is highly desirable in vaccine design, the extent to which growth in grm mimicked in vivo growth was examined. antibodies specific to in vivo grown cells partially labeled t ...19958590408
aeromonas encheleia sp. nov., isolated from european eels.four strains isolated from european eels in valencia, spain, were found to constitute a dna relatedness group which is 0 to 50% related to the 13 species and dna group 11 of the genus aeromonas. phenotypically, these strains have all of the properties that define the genus aeromonas. however, they differ from the previously described aeromonas species by three or more properties. the strains are positive for motility, growth at 37 degrees c, indole production, and arginine dihydrolase activity. ...19958590673
aeromonas hydrophila--its implications in freshwater injuries.lacerations or puncture wounds sustained in freshwater environments are susceptible to contamination by aeromonas hydrophila. numerous cases have been reported of cellulitis secondary to water-related injuries requiring hospitalization where a. hydrophila was the isolated organism. the typical presentation of an infection of a. hydrophila mimics a streptococcal soft tissue infection, which may result in delay in administration of appropriate antibiotics. a case is presented of a nonimmunocomprom ...19958590878
vibrio spp. secrete proaerolysin as a folded dimer without the need for disulphide bond formation.proaerolysin is an extracellular dimeric protein that is secreted across the inner and outer membranes of aeromonas spp. in separate steps. to investigate the role of protein folding in the second step, one or more cysteine residues were introduced and the mutant proaerolysins were expressed in aeromonas hydrophila and aeromonas salmonicida, as well as vibrio cholerae. replacing met-41 with cys resulted in expression of a protein that could form a dimer in which the monomers were linked together ...19958594324
isolation and identification of bacteria from petroleum derivatives contaminated soil.one of the aspects of bioremediation of petroleum derivatives contaminated soil using autochtonic microorganisms is isolation and identification of bacterial strains from polluted soil in order to choose the most active ones and to eliminate pathogens. in this research work some strains mainly from genus pseudomonas, acinetobacter, micrococcus, chryseomonas, agrobacterium and aeromonas were isolated from samples of soil contaminated with various petroleum derivatives. the population of bacteria ...19958934669
aeromonas species and plesiomonas shigelloides in diarrhoea in goa.sixty six strains (1.9%) of aeromonas species and 7 strains (0.2%) of plesiomonas shigelloides were isolated from 3484 stool/rectal swabs from cases of diarrhoea during the study period of four years from august 1986 to july 1990 in goa. although the selective medium (ampicillin sheep blood agar) was not used for the isolation of aeromonas, an increase in the rate of isolation of aeromonas from 0.2% in the year 1986 to 6.0% in 1990 was observed. stool samples from 100 controls (patients without ...19958919103
[water disinfection: comparative activities of ozone and chlorine on a wide spectrum of bacteria].ozone and chlorine are agents that disinfect by destroying, neutralizing or inhibiting the growth of pathogenic microorganisms. the treatment of drinking water with ozone has shown to be more efficient against spores of bacillus subtilis. it was observed that the ozone already in dose of 0.35 mg/l produced the reduction of at least 5 log in populations of approximately 1 x 10(6) cells/ml of escherichia coli, vibrio cholerae, salmonella typhi, yersinia enterocolitica, pseudomonas aeruginosa, aero ...19958850129
successful treatment of aeromonas hydrophila peritoneal dialysis-associated peritonitis using intermittent intraperitoneal gentamicin. 19958719554
detection of aerolysin gene in aeromonas strains isolated from drinking water, fish and foods by the polymerase chain reaction.a polymerase chain reaction (pcr) technique was used to assay the presence of the aerolysin gene in a total of 89 aeromonas hydrophila and a. sobria strains isolated from drinking water, fish and foods. these strains were also characterized for the production of virulence factors such as haemolysin, protease and cytotoxin. the primers used in the pcr targeted a 209-bp fragment of the aer gene coding for the beta-haemolysin and detected template dna only in haemolytic a. hydrophila strains. the c ...19957889729
fatal disease mimicking leptospirosis in a dog, caused by aeromonas hydrophila.a dog was treated for leptospirosis on clinical and epidemiological arguments. the amoxicillin treatment was not successful. pure culture of aeromonas hydrophila was then obtained from liver and kidney, indicating that the septicemia was due to this bacteria commonly found in waters.19957889733
haemolytic activity of aeromonas species isolated in libya.twenty seven aeromonas strains (5a. hydrophila, 8a. sobria and 14a. caviae) isolated from children with diarrhoea and 34 aeromonas strains (9a. hydrophila, 7a. sobria an 18a. caviae) isolated from children without diarrhoea were tested from haemolysin production. the results obtained showed that haemolysin production using human, horse or sheep erythrocytes was significantly associated with a.hydrophila and a sobria but not with a.caviae, regardless of whether these strains were isolated from ch ...19959074783
a transferable multiple drug resistance plasmid from vibrio cholerae o1.ten multiple antimicrobial-resistant isolates of vibrio cholerae o1 isolated from patients in uganda were characterized, and the transferability of resistance to bacteria of diverse origins was investigated. the isolates were toxigenic and belonged to biotype e1 tor, serotype ogawa, and ribotype 8, and possessed a 130-mda plasmid of incompatibility group 6-c. this plasmid, designated prvc1, was shown to confer resistance to trimethoprim (mediated by a dhfri gene), sulfonamides (a suii gene), tet ...19959158776
comparison of bgb-mug and lstb-mug in microbiological surveillance of recreational waters.recreational water surveillance is an important tool to prevent health hazards for the population. therefore distinct guide and imperative values for fecal indicators are listed in the ec directive about water quality control. the detection methods, however, give laboratories some room to choose their own method, which has led to difficulties in the comparability of results. in 1989 an ad-hoc working group of the coastal countries of germany established detection methods, which by now are obliga ...19959353533
[effectiveness of modified atmospheres against psychrotrophic pathogenic microorganisms in proteinaceous food].modified atmosphere packaging (map) of proteinaceous raw foods (meat, poultry and fish) extends their shelf-lives. it is well established that modified atmospheres (ma) inhibit the psychotropic aerobic gram-negative bacteria, the main spoilage microflora of proteinaceous raw foods stored under refrigeration. several researchers have warned about the possible growth of food poisoning microorganisms on them. considering the minimal growth temperatures of pathogens, this review only deals with aero ...19957546447
lactic acid inhibition of the growth of spoilage bacteria and cold tolerant pathogens on pork.the antibacterial effects of a 3% solution of lactic acid at 55 degrees c were assessed, by examining aerobic bacterial growth on artificially-inoculated pork fat and lean tissue. discs of fat or lean tissues, each of 10 cm2 surface area, were aseptically excised from pork longissimus dorsi muscle and inoculated with the cold tolerant pathogens listeria monocytogenes 4b scott a no. 3, yersinia enterocolitica 0:4,32 or aeromonas hydrophila atcc 7966, or with the wild type spoilage bacteria pseudo ...19957547145
buffering capacity and h+ membrane conductance of gram-negative bacteria.buffering capacity and membrane h+ conductance were examined in seven gram-negative species: aquaspirillum serpens, pseudomonas aeruginosa, alcaligenes faecalis, escherichia coli, salmonella typhimurium, proteus mirabilis and aeromonas hydrophila. all strains of enterobacteriaceae studied here showed a decrease in both parameters as the external ph increased, over the ph range studied. the other four species presented an increase in buffering capacity and membrane conductance to protons as the e ...19957557289
immunoregulatory activities of eicosanoids in the rainbow trout (oncorhynchus mykiss).eicosanoids such as prostaglandins (pg), leukotrienes (lt) and lipoxins (lx) have been shown to be potent immunoregulatory molecules in mammals. to determine if they have similar roles in 'lower' animals, rainbow trout (oncorhynchus mykiss) were immunized with either sheep erythrocytes or aeromonas salmonicida in the presence or absence of the stable analogue of pge2, 16,16-dimethyl-pge2, and the number of plaque-forming cells (pfc) or specific antibody levels determined. the higher dose of 16,1 ...19957558126
motile aeromonas as agent of infections of the foot.motile aeromonas infections of the foot are caused mostly by post-traumatic incidence, occurring mostly during summer months. serious complications such as osteomyelitis and amputation can result if the infections go untreated or are inadequately treated. the role of each species of motile aeromonas in pathogenesis and response to antimicrobial agents is not well understood because of taxonomic uncertainty. as a group, motile aeromonas respond well to aminoglycosides, second-generation and third ...19957562462
[many problems in the diagnosis of colitis. 2 case reports]. 19957564651
electron microscopic examination of factors influencing the expression of filamentous surface structures on clinical and environmental isolates of aeromonas veronii biotype sobria.strains of aeromonas veronii biotype sobria isolated from clinical and environmental sources were examined for their expression of surface structures under a variety of culture conditions. when grown on solid media at 37 degrees c, more than 95% of bacteria from the majority of strains isolated from human diarrheal feces and chicken carcasses were non-piliated or expressed only a few pili of long, flexible morphology per cell. strains isolated from water or other foods were much more likely to e ...19957565173
risk of diarrheal disease in ecuadorian day-care centers.to determine the risk for diarrheal disease (dd) in day-care centers (dcc) for children residing in a poor urban slum area of quito, ecuador, compared with that for children from the same environment but cared for in their own residential home (rh), a prospective age-, sex- and locale-controlled study of dd was conducted, including 115 children in dcc and 115 in rh, ages 12 to 42 months. the overall incidence of dd was 46/1000 child weeks. diarrhea was more common in dcc than in rh (relative ris ...19957567291
[occurrence of parasites, bacteria, viruses and fungi in fish that are pathogenic to men and fish].parasitological examinations comprised above 20,000 fish which were searched for parasitic nematoda of anisakidae. it was evidenced that herring were infected with anisakid larvae in 8%, cod and flatfish in 4% and the eelpouts in as many as in 52%. the species that prevails in these fish were: anisakis simplex, contracaecum osculatum c, hysterothylacium auctum and pseudoterranova decipiens, respectively. in direction of the bacteria pathogenic to man 765 fish were examined; in 38% there were fou ...19957571631
interactions of biapenem with active-site serine and metallo-beta-lactamases.biapenem, formerly ljc 10,627 or l-627, a carbapenem antibiotic, was studied in its interactions with 12 beta-lactamases belonging to the four molecular classes proposed by r. p. ambler (philos. trans. r. soc. lond. biol. sci. 289:321-331, 1980). kinetic parameters were determined. biapenem was readily inactivated by metallo-beta-lactamases but behaved as a transient inhibitor of the active-site serine enzymes tested, although with different acylation efficiency values. class a and class d beta- ...19957574520
microflora and tetrodotoxin-producing bacteria in a gastropod, niotha clathrata.shellfish (niotha clathrata) were collected in both july and november from three locations in taiwan (pingtung, kaohsiung and chiai prefecture) and assayed for anatomical distribution of tetrodotoxin (ttx) and aerobic heterotrophic bacteria. pingtung specimens showed higher toxicity than those from kaohsiung and chiai, and did not show much seasonal variation. at each site, the total aerobic bacterial counts in november samples were higher than in july. the predominant genera were vibrio, pseudo ...19957590540
the interaction of trimethoprim and quinolones against gram-negative fish pathogens.the effect of combination of trimethoprim with other non-sulphonamide antibacterial agents, in particular oxolinic acid and nalidixic acid, was evaluated against gram-negative fish pathogens. the species included aeromonas salmonicida, yersinia ruckeri, some vibrio spp. and escherichia coli as a reference. the extent of synergy found by other workers with these substances against human gram-negative bacteria was not apparent here. some positive interaction between trimethoprim and oxolinic acid ...19957592108
rapd analysis of aeromonas salmonicida and aeromonas hydrophila.the randomly amplified polymorphic dna (rapd) technique was used to analyse the genetic differentiation of 13 strains of aeromonas salmonicida subsp. salmonicida, and seven strains of aer. hydrophila. reproducible profiles of genomic dna fingerprints were generated by polymerase chain reaction (pcr) using a single randomly designed primer. the rapd profiles of all the non-motile aeromonads, aer. salmonicida subsp. salmonicida were identical. however, profiles of the motile aeromonads, aer. hydro ...19957592114
[in vitro antibacterial activity of balofloxacin (blfx) against isolates from patients with bacterial enteritis].in vitro antibacterial activity of balofloxacin (blfx), a newly developed fluoroquinoline, was compared with that of norfloxacin (nflx), ofloxacin (oflx) and ciplofloxacin (cpfx). bacterial strains used in this experiment were freshly isolated from patients with infectious enteritis just before blfx therapy. the isolates were 43 strains of vibrio cholerae o1, 1 strain of campylobacter sp., 4 strains of aeromonas spp., 3 strains of plesiomonas shigelloides, 1 strain of vibrio mimicus and 1 strain ...19957594800
a specific puld homolog is required for the secretion of paracrystalline surface array subunits in aeromonas hydrophila.aeromonas hydrophila is an important pathogen of fish, and its high-virulence strains display a two-dimensional paracrystalline layer (s-layer) on their outermost surfaces. the nucleotide sequence of a 4.1-kb region located 700 bp upstream of the a. hydrophila tf7 s-layer protein gene (ahsa) has been determined. a sequence analysis of the region revealed the presence of three complete open reading frames ending in a gene encoding a 79.8-kda polypeptide that shows high homology to the puld family ...19957608063
cloning and primary structure of the chia gene from aeromonas caviae.the chia gene from aeromonas caviae encodes an extracellular chitinase, 865 amino acids long, that shows a high degree of similarity to chitinase a of serratia marcescens. expression in escherichia coli yielded an enzymatically active protein from which a leader sequence was removed, presumably during transport of the enzyme across the cell membrane.19957608101
pseudomonas aeruginosa cytotoxin: the asp197-gly-asp-tyr-his-tyr-his-tyr202 containing loop is critical for plasma membrane binding.the cytotoxin from pseudomonas aeruginosa is a pore-forming toxin that binds specifically to water channel-related molecules of the erythrocyte membrane. here, we have defined a domain, asp197-gly-asp-tyr-his-tyr202 of the cytotoxin, to be essential for receptor binding. cytotoxin point mutants from the recombinant gene carrying substitutions in the domain were characterized in terms of inhibiting the binding of radioiodinated natural cytotoxin to rat erythrocyte and producing cytotoxic effects ...19957609787
aeromonas hydrophila myonecrosis accompanying mucormycosis five years after bone marrow transplantation.a 36-year-old man, five years after bone marrow transplantation for aplastic anemia, was admitted with myonecrosis of the forearm after he had immersed his hand in sewage water several days prior to his admission. blood cultures and specimens taken from the necrotic tissue of the arm all grew aeromonas hydrophila. following extension of the infection, the patient underwent amputation of the arm but ultimately died of cerebral mucormycosis. the epidemiology of aeromonas infections is discussed an ...19957614968
yeast beta-glucan stimulates respiratory burst activity of atlantic salmon (salmo salar l.) macrophages.previous studies have shown that head kidney macrophages isolated from glucan injected rainbow trout (oncorhychus mykiss) and atlantic salmon (salmo salar l.) have increased ability to kill aeromonas salmonicida. the present work was aimed at investigating the in vitro effects of glucan on the respiratory burst and bactericidal potential of atlantic salmon head kidney macrophages. salmon macrophages were incubated for 1-7 days with various concentrations of yeast beta-glucan (macrogard) and test ...19957615137
aeromonas bacteremia: review of 59 episodes.fifty-nine episodes of bacteremia due to aeromonas species occurred within a 5-year period in one medical center in southern taiwan. underlying diseases in the 58 patients included hepatic cirrhosis (36%) and cancer (24%). patients with aeromonas bacteremia more often had underlying hepatic cirrhosis than did those with bacteremia due to other gram-negative bacilli. males (67%) outnumbered females. the cases appeared to cluster in the summer and fall months. thirty-two percent were polymicrobial ...19957620014
a conserved aeromonas salmonicida porin provides protective immunity to rainbow trout.a protein with an apparent m(r) of 28,000 was isolated from outer membrane preparations of aeromonas salmonicida a440. the protein was tested for the ability to form pores, using a planar lipid bilayer model membrane system. the protein appeared to be a monomer with a single-channel conductance in 1.0 m kcl of 1.96 ns and a cation/anion permeability ratio of 2.91 +/- 0.68. these data show that the porin channel is comparable in size to ompc and ompf of escherichia coli and is relatively nonselec ...19957622241
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