| high-performance liquid chromatography determination of residue levels on chicken carcasses treated with cetylpyridinium chloride. | cetylpyridinium chloride (cpc) has been found to be effective in reducing contamination of chicken carcasses from a variety of microorganisms, including escherichia coli o157:h7, salmonella typhimurium, campylobacter jejuni, aeromonas hydrophila, listeria monocytogenes, and staphylococcus aureus. a procedure has been developed to determine residue levels on chicken carcasses after cpc treatment. for the analysis, chicken carcasses were extracted with 95% ethanol. the cpc concentration in the ext ... | 1999 | 10406212 |
| 1-butaneboronic acid binding to aeromonas proteolytica aminopeptidase: a case of arrested development. | hydrolases containing two metal ions connected by a bridging ligand catalyze reactions important in carcinogensis, tissue repair, post-translational modification, control and regulation of biochemical pathways, and protein degradation. the aminopeptidase from aeromonas proteolytica serves as a paradigm for the study of such bridged bimetallic proteases since its three-dimensional structure is known to very high resolution and its catalytic reaction is amenable to spectroscopic examination. herei ... | 1999 | 10413478 |
| biosynthesis of polyhydroxyalkanoates (pha) by recombinant ralstonia eutropha and effects of pha synthase activity on in vivo pha biosynthesis. | recombinant strains of ralstonia eutropha phb 4, which harbored aeromonas caviae polyhydroxyalkanoates (pha) biosynthesis genes under the control of a promoter for r. eutropha phb operon, were examined for pha production from various alkanoic acids. the recombinants produced poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [p(3hb-co-3hhx)] from hexanoate and octanoate, and poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxypentano ate) [p(3hb-co-3hv-co-3hhp)] from pentanoate and nonanoate. one ... | 1999 | 10416652 |
| cloning, sequencing, and role in virulence of two phospholipases (a1 and c) from mesophilic aeromonas sp. serogroup o:34. | two different representative recombinant clones encoding aeromonas hydrophila lipases were found upon screening on tributyrin (phospholipase a1) and egg yolk agar (lecithinase-phospholipase c) plates of a cosmid-based genomic library of aeromonas hydrophila ah-3 (serogroup o34) introduced into escherichia coli dh5alpha. subcloning, nucleotide sequencing, and in vitro-coupled transcription-translation experiments showed that the phospholipase a1 (pla) and c (plc) genes code for an 83-kda putative ... | 1999 | 10417167 |
| the channel-forming toxin aerolysin neutralizes human immunodeficiency virus type 1. | aerolysin is a channel-forming toxin secreted by aeromonas spp. that binds to glycosyl phosphatidylinositol (gpi)-anchored proteins, such as thy-1, on sensitive target cells. receptor binding is followed first by oligomerization of the toxin and then by insertion of the oligomers into the membrane to form stable channels that disrupt the permeability barrier. human immunodeficiency virus type 1 (hiv-1) produced from t cells is known to incorporate thy-1 and other gpi-anchored proteins into its m ... | 1999 | 10417655 |
| hemolysin of aeromonas sobria stimulates production of cyclic amp by cultured cells. | hemolysin of aeromonas sobria possesses both cytotoxic activity against mammalian cells and enterotoxic activity. histopathological examination revealed that hemolysin causes diarrhea without damaging the intestinal epithelial cells. and the fluid accumulated in the mouse intestinal loop by the action of the hemolysin is watery. these observations indicated that the enterotoxic activity of hemolysin is not dependent on its cytotoxic activity. to clarify the mechanism of the enterotoxic action of ... | 1999 | 10418132 |
| co-expression of 3-ketoacyl-acp reductase and polyhydroxyalkanoate synthase genes induces pha production in escherichia coli hb101 strain. | the escherichia coli 3-ketoacyl-acp reductase gene (fabgec) was cloned using a pcr technique to investigate the metabolic link between fatty acid metabolism and polyhydroxyalkanoate (pha) production. three plasmids respectively harboring fabgec and the poly-3-hydroxyalkanoate synthesis genes phacac and phac1ps from aeromonas caviae and pseudomonas sp. 61-3 respectively were constructed and introduced into e. coli hb101 strain. on a two-stage cultivation using dodecanoate as the sole carbon sourc ... | 1999 | 10418145 |
| a-protein from achromogenic atypical aeromonas salmonicida: molecular cloning, expression, purification, and characterization. | achromogenic atypical aeromonas salmonicida is the causative agent of goldfish ulcer disease. virulence of this bacterium is associated with the production of a paracrystalline outer membrane a-layer protein. the species-specific structural gene for the monomeric form of a-protein was cloned into a pet-3d plasmid in order to express and produce a recombinant form of the protein in escherichia coli bl21(de3). the induced protein was isolated from inclusion bodies by a simple solubilization-renatu ... | 1999 | 10425160 |
| preliminary screening of ethnomedicinal plants from india. | antibacterial activity of aqueous residues of 16 different ethnomedicinal plants have been studied. the effect of the aqueous extract at two different weights of plant residues, 30 and 40 mg, were tested against three gram positive bacteria and seven gram negative bacteria by the filter paper disc diffusion method. among the tested plants, cleome gynandropsis and ageratum conyzoides showed a significant control of the growth of alkaligens viscolactis, klebsiella aerogenas, bacillus cerues and st ... | 1999 | 10433484 |
| antimicrobial activity of essential oils and other plant extracts. | the antimicrobial activity of plant oils and extracts has been recognized for many years. however, few investigations have compared large numbers of oils and extracts using methods that are directly comparable. in the present study, 52 plant oils and extracts were investigated for activity against acinetobacter baumanii, aeromonas veronii biogroup sobria, candida albicans, enterococcus faecalis, escherichia col, klebsiella pneumoniae, pseudomonas aeruginosa, salmonella enterica subsp. enterica s ... | 1999 | 10438227 |
| efficacy of orally administered oxolinic acid and vetoquinol, an oxolinic acid ester, for the treatment of furunculosis in atlantic salmon salmo salar held in seawater. | this study was performed to determine the efficacy of orally administered oxolinic acid and vetoquinol, an oxolinic acid ester, in the treatment of experimental induced furunculosis in atlantic salmon salmo salar held in seawater. two strains of the causative bacterium aeromonas salmonicida subsp. salmonicida, 1 sensitive (vi-88/09/03175) and 1 resistant (3475/90) to oxolinic acid, were used. in 2 trials, cohabitational challenges were performed by introducing 8 fish challenged in advance by an ... | 1999 | 10439903 |
| molecular cloning and expression in escherichia coli of the extracellular endoprotease of aeromonas caviae t-64, a pro-aminopeptidase processing enzyme(1). | pa protease (pro-aminopeptidase processing protease) activates the pro-aminopeptidases from aeromonas caviae t-64 and vibrio proteolytica by removal of their pro-regions. cloning and sequencing of the pa protease gene revealed that pa protease was translated as a preproprotein consisting of four domains: a signal peptide; an n-terminal propeptide; a mature region; and a c-terminal propeptide. the deduced amino acid sequence of the pa protease precursor showed significant homology with several ba ... | 1999 | 10446382 |
| the prevalence of aeromonas species in feces of horses with diarrhea. | feces collected from 40 horses with diarrhea and 34 horses without diarrhea were examined to determine if an association existed between isolation of aeromonas spp. and diarrhea. samples were also examined for salmonella spp., and identification of viruses and parasite ova. neither salmonella spp. nor aeromonas spp. were isolated from the feces of 34 control horses. aeromonas spp. were isolated from feces of 22 of 40 (55%) horses with diarrhea. salmonella spp. were isolated from feces of 8 (20%) ... | 1999 | 10449228 |
| aeromonas hydrophila bacteremia acquired from an infected swimming pool. | | 1999 | 10451187 |
| interleukin-1 mediates hemodynamic dysfunction and release of eicosanoids and tumor necrosis factor during graded bacteremia. | the pathophysiologic events of sepsis mediated by interleukin-1 (il-1) remain ill-defined. the purpose of this study was to identify the circulatory derangements of which il-1 was a necessary mediator and evaluate its interactions with tumor necrosis factor (tnf) and the eicosanoids during graded bacteremia. eleven adult female swine were anesthetized, mechanically ventilated, and monitored with pulmonary artery catheters and arterial lines; they received intravenously either saline vehicle (sep ... | 1999 | 10454832 |
| two genes from the capsule of aeromonas hydrophila (serogroup o:34) confer serum resistance to escherichia coli k12 strains. | the escherichia coli dh5alpha strain as well as other k12-derived strains are unable to produce o-specific lipopolysaccharide and are thus rough and serum-sensitive. one representative recombinant clone (cos-sr1) containing aeromonas hydrophila (serogroup o:34) chromosomal dna conferred serum resistance to e. coli k12 strains. genetic, biochemical, and immunological studies suggested that the two genes (orf1 and wcaj) identified in a subclone (pac-sr9) of cos-sr1 are necessary for the production ... | 1999 | 10466407 |
| aeromonas sobria infection with severe soft tissue damage and segmental necrotizing gastroenteritis in a patient with alcoholic liver cirrhosis. | a 49-year-old man, who had a 3-year history of liver dysfunction but had not been treated, was admitted to the hospital with a sudden onset of fever and generalized muscle pain. he subsequently developed generalized purpura with scattered hemorrhagic bullae of the skin and massive bloody stools. aeromonas sobria was proven by culture of both blood and bullous fluid. in spite of the extensive treatment with antibiotics and other medications in the intensive care unit (icu), the patient went into ... | 1999 | 10469397 |
| inhibition of the aminopeptidase from aeromonas proteolytica by aliphatic alcohols. characterization of the hydrophobic substrate recognition site. | seven aliphatic and two aromatic alcohols were tested as reporters of the substrate selectivity of the aminopeptidase from aeromonas proteolytica (aap). this series of alcohols was chosen to systematically probe the effect of carbon chain length, steric bulk, and inhibitor shape on the inhibition of aap. initially, however, the question of whether aap is denatured in the presence of aliphatic alcohols was addressed. on the basis of circular dichroism (cd), electronic absorption, and fluorescence ... | 1999 | 10471294 |
| bacterial populations associated with a sorghum-based fermented weaning cereal. | microbiological surveys, to determine the quality and safety, were conducted on 45 sorghum samples comprising dry powders (n = 15) and corresponding fermented (n = 15) and cooked fermented porridge (n = 15) samples collected from households in an informal settlement of the gauteng province of south africa. mean aerobic plate counts, gram-negative counts and bacterial spore counts of sorghum powder samples decreased in fermented and cooked fermented porridge samples. however, mean lactic acid bac ... | 1999 | 10477073 |
| effects of dimerized lysozyme (klp-602) on the cellular and humoral defence mechanisms in sheatfish (silurus glanis): in vitro and in vivo study. | this study examined the effects of the dimerized lysozyme (klp-602) on the immunocompetence cell activity in sheatfish (silurus glanis) and its influence in vivo on the non-specific defence mechanisms and protection against motile aeromonad septicaemia (mas). the in vitro study showed that the lysozyme dimer (klp-602), at concentrations between 5 and 50 micrograms/ml of medium significantly (p < 0.05) increased the respiratory burst activity and potential killing activity of pronephric macrophag ... | 1999 | 10478423 |
| effects of malathion on disease susceptibility in woodhouse's toads. | adult male woodhouse's toads (bufo woodhousi) developed clinical disease, hepatomegaly, and died at a higher rate when externally exposed once to either a high or low sublethal dose (0.011 or 0.0011 mg malathion/g toad) of field grade malathion and challenged with a sublethal dose of aeromonas hydrophila injected intraperintoneally (1.1 x 10(4) bacteria/g toad) when compared to toads not exposed to malathion but challenged with a. hydrophila (p < 0.007). toads exposed to malathion (high or low d ... | 1999 | 10479088 |
| clostridium septicum alpha toxin uses glycosylphosphatidylinositol-anchored protein receptors. | the alpha toxin produced by clostridium septicum is a channel-forming protein that is an important contributor to the virulence of the organism. chinese hamster ovary (cho) cells are sensitive to low concentrations of the toxin, indicating that they contain toxin receptors. using retroviral mutagenesis, a mutant cho line (bag15) was generated that is resistant to alpha toxin. facs analysis showed that the mutant cells have lost the ability to bind the toxin, indicating that they lack an alpha to ... | 1999 | 10480947 |
| differences in the pathogenesis of bacteria isolated from contact-lens-induced infiltrative conditions. | the purpose of this study was to examine the ability of gram-negative bacteria isolated from the non-infectious condition contact-lens-induced acute red eye (clare) and infectious microbial keratitis (mk) to infect the eyes of mice. | 1999 | 10484200 |
| major capsid proteins of certain vibrio and aeromonas phages are homologous to the equivalent protein, gp23(*), of coliphage t4. | n-terminal amino acid sequences of major capsid proteins (mcps) of three vibriophages (kvp20, kvp40 and nt-1), two aeromonad phages (aeh 1 and 65) and coliphage t4 were compared. all these phages are morphologically similar, belonging to family myoviridae and the vernacular genus name "t4-like phages". a dendrogram constructed from homology data indicated that (i) the three vibriophages were closely related, (ii) the two aeromonad phages were also fairly related and (iii) these five phages were ... | 1999 | 10486118 |
| diverse restriction fragment length polymorphism patterns of the pcr-amplified 16s rrna genes in aeromonas veronii strains and possible misidentification of aeromonas species. | restriction fragment length polymorphism analysis after pcr amplification (rflp-pcr) of the 16s rrna gene has been previously proposed as a rapid method to identify aeromonas species. in the present study, the precision of rflp-pcr was evaluated with 62 aeromonas reference strains. the analysis revealed that aeromonas veronii biovar sobria strains produce various patterns, possibly leading to its misidentification as an environmental species. for most other aeromonas species little variation was ... | 1999 | 10488176 |
| antibiotic response and plasmid profile of bacteria isolated from a landfill. | bacteria isolates belonging to the genera bacillus, corynebacterium, aeromonas, and enterobacter were isolated from a municipal waste landfill in durham, nc. bacterial counts obtained with three general purpose media were log(10) colony-forming units (cfu)/g of 9.30, 9. 26, and 9.20 respectively for plate count agar, brain heart infusion agar, and nutrient agar. coliform count from macconkey agar was log(10) 7.28/g sample. isolates were generally sensitive to tetracycline and chloramphenicol (13 ... | 1999 | 10489432 |
| numbers and species of motile aeromonads during the manufacture of naturally contaminated spanish fermented sausages (longaniza and chorizo). | the prevalence of aeromonads in the initial mixes of 14 batches of chorizo and longaniza obtained from three small- and middle-sized factories was 78.5%, with counts ranging from >1.00 to 4.47 log10 cfu/g. only 2 of 10 mixture samples prepared at a large and modern processing plant yielded these organisms, with levels below 1 log10 cfu/g. the hygienic status of factories significantly affected incidence and counts. of 39 presumptive isolates from glutamate starch penicillin agar, 36 were confirm ... | 1999 | 10492481 |
| escherichia coli in patients with acute gastroenteritis in lagos, nigeria. | over a period of 12 months, a total of 852 stool samples from patients (both children and adults) with acute diarrhoeal diseases attending some public and government recognised health institutions in lagos metropolis were screened for diarrhoeagenic bacterial agents. one hundred and eighty two bacterial isolates were found out of which 83 (45.6%) were escherichia coli, 38 (20.9%) shigella spp, 31 (17%) salmonella spp, 16 (8.8%) klebsiella spp, eight (4.4%) aeromonas spp, while there were only si ... | 1998 | 10493052 |
| quorum sensing-dependent regulation and blockade of exoprotease production in aeromonas hydrophila. | in aeromonas hydrophila, the ahyi gene encodes a protein responsible for the synthesis of the quorum sensing signal n-butanoyl-l-homoserine lactone (c4-hsl). inactivation of the ahyi gene on the a. hydrophila chromosome abolishes c4-hsl production. the exoprotease activity of a. hydrophila consists of both serine protease and metalloprotease activities; in the ahyi-negative strain, both are substantially reduced but can be restored by the addition of exogenous c4-hsl. in contrast, mutation of th ... | 1999 | 10496895 |
| functional characterization of type iv pili expressed on diarrhea-associated isolates of aeromonas species. | our past work has shown that long, flexible type iv pili (single or in bundles) are the predominant pili expressed on fecal isolates of diarrhea-associated species of aeromonas (aeromonas veronii biovar sobria and a. caviae). they represent a family of type iv pili which we have designated bfp (for bundle-forming pili). reports from japan suggest that bfp are intestinal colonization factors. this study presents compelling evidence to support this conclusion. aeromonas bacteria and/or bfp purifie ... | 1999 | 10496928 |
| synthesis and sar of thioester and thiol inhibitors of imp-1 metallo-beta-lactamase. | potent thioester and thiol inhibitors of imp-1 metallo-beta-lactamase have been synthesized employing a solid-phase mitsunobu reaction as the key step. | 1999 | 10498206 |
| xynx, a possible exo-xylanase of aeromonas caviae me-1 that produces exclusively xylobiose and xylotetraose from xylan. | a gene, xynx, encoding a novel xylanase, was cloned from aeromonas caviae me-1. this gene encoded an enzyme that was constituted of 334 amino acid residues (38,580 da) and was similar in sequence to family 10 (family f) beta-1,4 endo-xylanases. xynx produced only xylobiose and xylotetraose from birch wood xylan, and xylotriose, xylopentaose, and higher oligosaccharides were not detected in the tlc analysis. we designated it as x2/x4-forming xylanase. this enzyme does not have transglycosylation ... | 1999 | 10500996 |
| identification and characterization of the aeromonas sobria hemolysin glycoprotein receptor on intestine 407 cells. | aeromonas sobria hemolysin is important in the pathogenesis of diarrhoea caused by this enteropathogenic bacterium. by immunoprecipitation analysis using hemolysin and anti-hemolysin antibody, a 66 kda protein (p66) was identified as a receptor for a. sobria hemolysin on intestine 407 cells. treatment of p66 with n-glycosidase f reduced the apparent sized of p66 to 60 kda on sds-polyacrylamide gels. p66, released from intestine 407 cells following incubation with phosphatidylinositol-specific ph ... | 1999 | 10502462 |
| carbapenem derivatives as potential inhibitors of various beta-lactamases, including class b metallo-beta-lactamases. | a variety of 1beta-methylcarbapenem derivatives were screened to identify inhibitors of imp-1 metallo-beta-lactamase, a class b beta-lactamase, in an automated microassay system using nitrocefin as a substrate. the structure-inhibitory-activity relationship study revealed that three types of 1beta-methylcarbapenems having benzothienylthio, dithiocarbamate, or pyrrolidinylthio moieties at the c-2 position showed good inhibitory activity. among the compounds screened, j-110,441, having a benzothie ... | 1999 | 10508031 |
| case-control study of enteropathogens associated with childhood diarrhea in dhaka, bangladesh. | the international centre for diarrhoeal disease research, bangladesh, is a major center for research into diarrheal diseases. the center treats more than 100,000 patients a year. to obtain useful information representative of all patients, a surveillance system in which a 4% systematic sample of all patients is studied in detail, including etiological agents of diarrhea, was installed in october 1979. the first paper on etiology for the surveillance patients was published in 1982, which identifi ... | 1999 | 10523534 |
| behavior of listeria monocytogenes and aeromonas spp. on fresh-cut produce packaged under equilibrium-modified atmosphere. | storage experiments were conducted to follow the behavior of pathogens on fresh-cut vegetables (trimmed brussels sprouts, grated carrots, shredded iceberg lettuce, and shredded chicory endives) packaged under an equilibrium-modified atmosphere (ema) (2 to 3% o2, 2 to 3% co2, and 94 to 96% n2) and stored at 7 degrees c. as a comparison, fresh-cut vegetables were also packaged in a perforated high-barrier film (air conditions) and stored at 7 degrees c. in a first step, the shelf life of the veget ... | 1999 | 10528715 |
| flagellate predation on a bacterial model community: interplay of size-selective grazing, specific bacterial cell size, and bacterial community composition. | the influence of grazing by the bacterivorous nanoflagellate ochromonas sp. strain ds on the taxonomic and morphological structures of a complex bacterial community was studied in one-stage chemostat experiments. a bacterial community, consisting of at least 30 different strains, was fed with a complex carbon source under conditions of low growth rate (0.5 day(-1) when nongrazed) and low substrate concentration (9 mg liter(-1)). before and after the introduction of the predator, the bacterial co ... | 1999 | 10543797 |
| cell adhesion of plesiomonas shigelloides. | plesiomonas shigelloides evidences an inconsistent behaviour in the int-407 cell adhesion test. isolates from clinical material evidence regular and pronounced cell adhesion which, however, is low compared with that of clinical aeromonas isolates. in some of the p. shigelloides strains isolated from clinical material, the cell adhesion is markedly more pronounced than in others. the p. shigelloides strains isolated from the natural habitat from both pond water, mud samples and water insects mani ... | 1999 | 10546328 |
| [three cases of aeromonas hydrophila septicemia complicated with hepatic cirrhosis]. | | 1999 | 10548017 |
| unusual food-borne pathogens. listeria monocytogenes, aeromonas, plesiomonas, and edwardsiella species. | although these four groups of organisms are perceived as infrequent food-borne pathogens or of dubious significance, increasing epidemiologic data indicate that l. monocytogenes is an emerging cause of infections, particularly gastroenteritis. furthermore, if data are ever generated that prove that most fecal isolates of aeromonas are involved in bacterial diarrhea, then aeromonads will become recognized as important food-borne pathogens. for plesiomonas and edwardsiella, recognition of possible ... | 1999 | 10549426 |
| polymerase chain reaction (pcr)-based typing analysis of atypical isolates of the fish pathogen aeromonas salmonicida. | two hundred and five isolates of atypical aeromonas salmonicida, recovered from a wide range of hosts and countries were characterized by polymerase chain reaction (pcr) targeting four genes. the chosen genes were those encoding the extracellular a-layer protein (ap), the serine protease (sprot), the glycerophospholipid:cholestrol acetyltransferase protein (gcat), and the 16s rrna (16s rdna). all the atypical a. salmonicida isolates could be assigned to 4 pcr groups. group 1 comprised 45 strains ... | 1999 | 10553293 |
| phylogenetic positions of aeromonas encheleia, aeromonas popoffii, aeromonas dna hybridization group 11 and aeromonas group 501. | the 16s rdna sequences of the recently described aeromonas encheleia and aeromonas popoffii, were determined and compared with data from all known aeromonas sp. diagnostic 16s rdna regions were also sequenced for some strains previously considered as an extension of a. encheleia and a strain of aeromonas group 501 (formerly enteric group 501). results indicated that a. encheleia and a. popoffii are phylogenetically separated species as originally described. a conclusion about hg11 taxonomic stat ... | 1999 | 10555319 |
| carnobacterium inhibens sp. nov., isolated from the intestine of atlantic salmon (salmo salar). | strain k1t, isolated from the gastrointestinal tract of atlantic salmon (salmo salar), has the capacity to inhibit the growth of the fish pathogens vibrio anguillarum and aeromonas salmonicida. strain k1t is a motile gram-positive psychrophilic rod that lacks both catalase and oxidase, which does not grow on acetate containing media, but grows at ph 9 and in tsb with up to 6% sodium chloride content. strain k1t is facultatively anaerobic and tryptone as a sole source of nutrient promotes growth. ... | 1999 | 10555373 |
| [aeromonas hydrophila septicemia with necrotizing myofascitis in a patient with hypoplastic leukemia]. | a 54-year-old male was admitted to kawasaki medical school hospital with the complaint of fever. his diagnosis of hypoplastic leukemia had been made one year ago. after the admission, cecal mass with pain and high fever were noted. four days later, he suddenly lost consciousness and died. aeromonas hydrophila was isolated from blood cultures and also from the myofascitis specimen. autopsy specimen of the iliopsoas muscle showed necrotizing myofascitis. the specimen obtained from the cecum showed ... | 1999 | 10565125 |
| slow-binding inhibition of the aminopeptidase from aeromonas proteolytica by peptide thiols: synthesis and spectroscopic characterization. | peptide-derived thiols of the general structure n-mercaptoacyl-leucyl-p-nitroanilide (1a-c) were synthesized and found to be potent, slow-binding inhibitors of the aminopeptidase from aeromonas proteolytica (aap). the overall potencies (k(i)) of these inhibitors against aap range from 2.5 to 57 nm exceeding that of the natural product bestatin and approaching that of amastatin. the corresponding alcohols (2a-b) are simple competitive inhibitors of much lower potencies (k(i) = 23 and 360 microm). ... | 1999 | 10569943 |
| enterotoxins in aeromonas-associated gastroenteritis. | aeromonas species produce an array of virulence factors, and the pathogenesis of aeromonas infections is therefore complex and multifactorial. aeromonas-associated gastroenteritis is especially a problem in young children. the potential involvement of enterotoxins in the pathogenesis of aeromonas infections is discussed. | 1999 | 10572317 |
| occurrence of haemolytic & cytotoxic aeromonas species in domestic water supplies in chennai. | a study on the occurrence of aeromonas species in the domestic water supplies in chennai showed that as much as 37.9 per cent of the water samples analyzed from various sources harbored aeromonas spp. majority of the isolates belonged to aeromonas sobria (13.7%), a. caviae (11.6%) and a. hydrophila (9.5%). among the 37 metropolitan water samples analyzed, 11 samples yielded aeromonas spp. inclusive of three isolates of a. hydrophila, four of a. sobria and two isolates each of a. caviae and a. ja ... | 1999 | 10573655 |
| platelet-activating factor and arachidonic acid metabolites mediate tumor necrosis factor and eicosanoid kinetics and cardiopulmonary dysfunction during bacteremic shock. | platelet-activating factor (paf) and eicosanoids are putative mediators of septic shock that are associated with release of tumor necrosis factor (tnf). the purpose of this investigation was to a) examine temporal patterns of tnf and arachidonic acid metabolite release in a porcine model of bacteremic shock and b) selectively block paf, thromboxane a2, prostacyclin, and leukotrienes to determine the relationships among these inflammatory response mediators and the alterations in cardiorespirator ... | 1999 | 10579269 |
| genetic diversity of atypical aeromonas salmonicida studied by pulsed-field gel electrophoresis. | pulsed-field gel electrophoresis (pfge) pattern analysis with xbai restriction enzyme was used to study the genetic heterogeneity of 88 atypical aeromonas salmonicida strains which were earlier or during this study characterized phenotypically, by ribotyping (clai/psti) and by plasmid profile analysis. the strains of certain'ribotypes were also analysed by digestion with spei. the strains represented different geographic locations: finland (72 strains), iceland (5 strains), norway (5 strains), s ... | 1999 | 10579451 |
| the abca subclass of mammalian transporters. | we describe here a subclass of mammalian abc transporters, the abca subfamily. this is a unique group that, in contrast to any other human abc transporters, lacks a structural counterpart in yeast. the structural hallmark of the abca subfamily is the presence of a stretch of hydrophobic amino acids thought to span the membrane within the putative regulatory (r) domain. as for today, four abca transporters have been fully characterised but 11 abca-encoding genes have been identified. abca-specifi ... | 1999 | 10581369 |
| [case of infectious hepatic cyst caused by aeromonas caviae]. | | 1999 | 10581795 |
| monoclonal antibodies against asap1, a major exotoxin of the fish pathogen aeromonas salmonicida subsp. achromogenes, and their application in elisa. | two monoclonal antibodies (mabs) binding to a toxic extracellular metallo-proteinase of aeromonas salmonicida subsp. achromogenes, asap1, were produced. both reacted with common epitopes of the native enzyme and recognized this 20 kda antigen on western blots. one of these mabs had an inhibitory effect on the caseinase activity of the exotoxin. a mab-based elisa was set up and evaluated for serological detection of asap1 in bacterial culture filtrates. the exotoxin was identified serologically i ... | 1999 | 10583691 |
| survival ability of cytotoxic strains of motile aeromonas spp. in different types of water. | the ability of motile aeromonas spp. to survive in drinking water (mineral and tap water) and in sea water was experimentally tested. clinically isolated cytotoxic strains of a. hydrophila, a. caviae and a. sobria were selected for this study. after contamination of water samples, the survival of aeromonas strains was studied for at least three months using viable counts. the results obtained show that the survival of the aeromonas spp. varies considerably depending on species and water type. fo ... | 1999 | 10583745 |
| a comparison of different culture media for the membrane filter quantification of aeromonas in water. | a comparative assessment of culture media for the membrane filter enumeration of aeromonas spp. in water was performed, testing the effects of different incubation conditions (aerobic and anaerobic), temperatures (30 and 37 degrees c) and times (24 and 48 h). different water samples seeded with test suspensions of aeromonas spp., fecal material or raw sewage were examined. results indicate clearly that plates should be incubated aerobically at 30 degrees c for 24 h. if the bacterial contaminatio ... | 1999 | 10583754 |
| pcr detection, characterization, and distribution of virulence genes in aeromonas spp. | we found 73.1 to 96.9% similarity by aligning the cytolytic enterotoxin gene of aeromonas hydrophila ssu (ahcytoen; genbank accession no. m84709) against aerolysin genes of aeromonas spp., suggesting the possibility of selecting common primers. identities of 90 to 100% were found among the eight selected primers from those genes. amplicons obtained from aeromonas sp. reference strains by using specific primers for each gene or a cocktail of primers were 232 bp long. of hybridization group 4/5a/5 ... | 1999 | 10583979 |
| hemolytic activity and siderophore production in different aeromonas species isolated from fish. | the hemolytic activity and siderophore production of several strains of motile aeromonads were determined. the hemolytic activity of aeromonas caviae and aeromonas eucrenophila was enhanced after trypsinization of the samples. the enhancement of hemolysis was observed in strains that carried an aerolysin-like gene, detected by a pcr procedure. siderophore production was demonstrated in all but one strain of aeromonas jandaei. no apparent relationship was observed between the presence of plasmid ... | 1999 | 10584028 |
| skip colonic ulceration in typhoid ileo-colitis. | colonic skip lesions are typically described in crohn's colitis, but this phenomenon has been recognized in ulcerative colitis (skipped appendiceal involvement), behcet's colitis, cytomegaloviral colitis, and even in aeromonas hydrophilia and histoplasma capsulatum infection. however, skip lesions in typhoid ileo-colitis have not been reported in the english-language literature. we report herein a patient with skip ulcers due to typhoid fever. | 1999 | 10588187 |
| production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by high-cell-density cultivation of aeromonas hydrophila. | the newly screened aeromonas hydrophila produces copolymer consisting of 3-hydroxybutyrate (3hb) and 3-hydroxyhexanoate (3hhx). the characteristics of cell growth and polymer accumulation were examined using various carbon sources. p(3hb-co-3hhx) was produced from lauric acid and oleic acid only. p(3hb-co-3hhx) content can be increased by limitation of phosphorus. a maximal p(3hb-co-3hhx) content of 28.8 wt% could be obtained in flask culture. by applying the optimally designed nutrient feeding ... | 2000 | 10592522 |
| survey on the distribution of vibrionaceae at the seaport areas in taiwan, 1991-1994. | a monthly survey on the distribution of human-pathogenic vibrionaceae of the seawater from five principal harbors in taiwan was conducted by national quarantine service from july, 1991 to february, 1994. of the total 1,167 vibrionaceae isolates, strains of vibrio alginolyticus (449 strains) were the most frequently isolated, followed by vibrio parahaemolyticus (262) , aeromonas hydrophila (153), vibrio cholerae non-o1 (86), and vibrio vulnificus (67). none of vibrio cholerae o1 was isolated. the ... | 1996 | 10592802 |
| amine borate catabolism by bacteria isolated from contaminated metal-working fluids | four bacterial strains (tentatively identified as strains of aeromonas, pseudomonas, flavobacterium and bacillus) isolated from contaminated metal-working fluids were assayed for the capacity to utilize the borate derivatives of monoethanolamine (mea), diethanolamine (dea) and triethanolamine (tea). two of these strains, isolates av1 (flavobacterium) and cl1 (bacillus) were capable of growth on each of the borate esters with cell yields of 0.6 gl - 1 for av1 cultured on dea- and tea-borate, 0.3- ... | 1999 | 10594706 |
| the extracellular regions of psma and the transferrin receptor contain an aminopeptidase domain: implications for drug design. | the aeromonas proteolytica aminopeptidase (amp), pseudomonas sp. (rs-16) carboxypeptidase g2 (cpg2), and streptomyces griseus aminopeptidase (sgap) are zinc dependent proteolytic enzymes with cocatalytic zinc ion centers and a conserved aminopeptidase fold. a blast search with the sequence of the solved amp structure indicated that a similar domain could be found in prostate-specific membrane antigen (psma) and the transferrin receptor (tfr). when the psma or tfr sequence was input into the thre ... | 1999 | 10595564 |
| aeromonas abscess in an immunocompromised child. | an 11-year-old immunocompromised child developed cellulitis and abscess due to aeromonas hydrophila at the site of bone marrow aspiration after swimming in a freshwater lake. the patient required treatment with intravenous antibiotics and surgical debridement to eradicate the infection. both common and unusual organisms may complicate infections at the sites of percutaneous procedures. | 1999 | 10598672 |
| aeromonas-associated diarrhoea in bangladeshi children: clinical and epidemiological characteristics. | we studied the clinical and epidemiological features associated with aeromonas diarrhoea by a hospital survey of 7,398 children under 5 years of age presenting with diarrhoea. the data were actually based upon two cohorts from this survey, the majority of the data being identified from 405 (5.5%) in whom aeromonas was the sole enteric pathogen. aeromonas caviae was the most prevalent species, accounting for 32% (129/405) of all isolates. eighty-three per cent of children with aeromonas-associate ... | 1999 | 10605516 |
| size distribution of bacterial and fungal bioaerosols in indoor air. | the aim of this study was to determine the size distribution of bacteria and fungi occurring in the air of human dwellings. the concentration and size distribution of particulate aerosol, gram-positive mesophilic bacteria, gram-negative mesophilic bacteria and fungi were examined in 60 flats situated in the upper silesia conurbation, southern poland. the investigated flats comprised three quantitatively equal (20 flats each) groups: flats without additional emission sources of particulate aeroso ... | 1999 | 10607991 |
| dimer dissociation of the pore-forming toxin aerolysin precedes receptor binding. | the pore-forming toxin aerolysin is secreted by aeromonas hydrophila as an inactive precursor. based on chemical cross-linking and gel filtration, we show here that proaerolysin exists as a monomer at low concentrations but is dimeric above 0.1 mg/ml. at intermediate concentrations, monomers and dimers appeared to be in rapid equilibrium. all together our data indicate that, at low concentrations, the toxin is a monomer and that this species is competent for receptor binding. in contrast, a muta ... | 1999 | 10608828 |
| recent developments in fish vaccinology. | during the last 10 to 20 years vaccination has become established as an important method for prevention of infectious diseases in farmed fish, mainly salmonid species. so far, most commercial vaccines have been inactivated vaccines administered by injection or immersion. bacterial infections caused by gram-negative bacteria such as vibrio sp., aeromonas sp., and yersinia sp. have been effectively controlled by vaccination. with furunculosis, the success is attributed to the use of injectable vac ... | 1999 | 10614510 |
| convenient test for screening metallo-beta-lactamase-producing gram-negative bacteria by using thiol compounds. | a simple disk diffusion test was constructed for detection of imp-1-type metallo-beta-lactamase-producing gram-negative bacteria. two kirby-bauer disks containing ceftazidime (caz) and a filter disk containing a metallo-beta-lactamase inhibitor were used in this test. several imp-1 inhibitors such as thiol compounds including 2-mercaptopropionic acid, heavy metal salts, and edta were evaluated for this test. two caz disks were placed on a mueller-hinton agar plate on which a bacterial suspension ... | 2000 | 10618060 |
| impact of an urban effluent on antibiotic resistance of riverine enterobacteriaceae and aeromonas spp. | in order to evaluate the impact of an urban effluent on antibiotic resistance of freshwater bacterial populations, water samples were collected from the arga river (spain), upstream and downstream from the wastewater discharge of the city of pamplona. strains of enterobacteriaceae (representative of the human and animal commensal flora) (110 isolates) and aeromonas (typically waterborne bacteria) (118 isolates) were selected for antibiotic susceptibility testing. most of the aeromonas strains (7 ... | 2000 | 10618213 |
| carboxy terminal region of haemolysin of aeromonas sobria triggers dimerization. | haemolysin of aeromonas sobria is released into the culture supernatant in the form of prohaemolysin. removal of a 42 amino acid peptide at the carboxy-terminal end converts prohaemolysin into mature haemolysin. as the role of the peptide removed from the mature haemolysin has not been studied, we mutated the haemolysin genes to delete several amino acid residues from the carboxy terminus, expressed the mutant genes in a. sobria and analysed the haemolysins produced. deletion of more than three ... | 2000 | 10623561 |
| two regions of epsl involved in species-specific protein-protein interactions with epse and epsm of the general secretion pathway in vibrio cholerae. | extracellular secretion of proteins via the type ii or general secretion pathway in gram-negative bacteria requires the assistance of at least 12 gene products that are thought to form a complex apparatus through which secreted proteins are translocated. although this apparatus is specifically required only for the outer membrane translocation step during transport across the bacterial cell envelope, it is believed to span both membranes. the epse, epsl, and epsm proteins of the type ii apparatu ... | 2000 | 10633109 |
| glycine metabolism in candida albicans: characterization of the serine hydroxymethyltransferase (shm1, shm2) and threonine aldolase (gly1) genes. | genes encoding the mitochondrial (shm1) and cytosolic (shm2) serine hydroxymethyltransferases, and the l-threonine aldolase gene (gly1) from candida albicans were cloned and sequenced. all three genes are involved in glycine metabolism. the c. albicans shm1 protein is 82% identical to that from saccharomyces cerevisiae and 56% identical to that from homo sapiens. the corresponding identities for the shm2 proteins are 68% and 53%. the gly1 protein shares significant identity with the s. cerevisia ... | 2000 | 10641038 |
| quality of water--quality of life. | especially in developing countries, the problem of adequate drinking water supply is an ever growing one. public health programmes have been established to improve the population's health conditions, but these programmes require big financial means for guaranteeing adequate supply of potable water and medical therapy for sick people. too little emphasis is still put on regular testing of drinking water for microorganisms such as aeromonas sp. and vibrio sp. in a spot check analysis in various co ... | 1999 | 10659386 |
| prevalence and diversity of aeromonas and vibrio spp. in coastal waters of southern italy. | a survey was undertaken to examine sea water and sediment for the presence of vibrio and aeromonas spp. along approximately 900 km of coast in southern italy during early and late summer. a quantitative analysis was also done to evaluate the water fecal contamination at the stations examined. the results indicate that all the investigated areas were submitted to a wide spatial fluctuation of fecal contamination and that vibrio and aeromonas spp. were present in both high and low fecal-contaminat ... | 2000 | 10660258 |
| production of an enterotoxin by a gastro-enteritis-associated aeromonas strain. | the potential of motile aeromonas species to cause human gastrointestinal infections has been recognised recently. considerable worldwide epidemiological, microbiological and clinical investigations have shown that some strains of the different motile aeromonads are of increasing enteropathogenic significance, especially in children, the elderly and in immunocompromised individuals. some of the diarrhoeal symptoms of aeromonas-associated gastro-enteritis have been attributed to enterotoxins. in ... | 2000 | 10670562 |
| kinetic and spectroscopic characterization of native and metal-substituted beta-lactamase from aeromonas hydrophila ae036. | two metal ion binding sites are conserved in metallo-beta-lactamase from aeromonas hydrophila. the ligands of a first zinc ion bound with picomolar dissociation constant were identified by exafs spectroscopy as one cys, two his and one additional n/o donor. sulfur-to-metal charge transfer bands are observed for all mono- and di-metal species substituted with cu(ii) or co(ii) due to ligation of the single conserved cysteine residue. binding of a second metal ion results in non-competitive inhibit ... | 2000 | 10675542 |
| cytotoxicity of non o1, non o139 vibrios isolated from fresh water bodies in vellore, south india. | the samples of plankton, soil sediment and water from a pond, a lake and a moat respectively in and around vellore were studied for environmental vibrios. vibrios were isolated from all these specimens after enrichment in alkaline peptone water and subculture on selective media. non o1, non o139 vibrio cholerae, aeromonas spp. and plesiomonas spp. were isolated. there were no isolates of v. cholerae serogroup o1 and o139. representative strains of non o1 and non o139 v. cholerae from environment ... | 1999 | 10680299 |
| acute renal failure in an infant associated with cytotoxic aeromonas sobria isolated from patient's stool and from aquarium water as suspected source of infection. | | 2000 | 10681210 |
| [cellulitis caused by aeromonas hydrophila]. | we report the case of a cirrhotic patient with leukocytoclastic vasculitis who developed a rapid and progressive cellulitis with hemorrhagic bulla and sepsis due to aeromonas hydrophila, the portal of entry was the surgical leech of a cutaneous biopsy. | 1999 | 10686717 |
| detection, prevalence, purification and characterization of lecithinase of klebsiella pneumoniae. | lecithinase activity in klebsiella is a rare trait as out of 208 strains of klebsiella belonging to 3 species, viz. k. pneumoniae (168), k. planticola (29) and k. oxytoca (11), only 4 strains of k. pneumoniae produced lecithinase positive colonies on egg-yolk-agar. although cell lysates of 16 k. pneumoniae yielded positive results for lecithinase assay on egg-yolk-agar, 19 strains were detected positive for lecithinase with elisa using anti-lecithinase serum. release of up to 52.12% cell-bound l ... | 1999 | 10687289 |
| isolation of bacteria other than helicobacter pylori from stomachs of squirrel monkeys (saimiri spp.) with gastritis. | gastric biopsy specimens obtained from 12 squirrel monkeys (saimiri spp.) were investigated by culture for the presence of bacteria. the stomachs of two monkeys with gastritis were colonized with gram-negative, urease-positive bacteria, identified as ochrobactrum anthropi by the vitek and api nft methods (biomérieux). a third monkey with gastritis was positive for aeromonas salmonicida and pseudomonas vesicularis (both urease-negative). no helicobacter pylori was isolated from squirrel monkeys. ... | 2000 | 10711437 |
| molecular cloning of two (r)-specific enoyl-coa hydratase genes from pseudomonas aeruginosa and their use for polyhydroxyalkanoate synthesis. | two pseudomonas aeruginosa genes, termed phaj1(pa) and phaj2(pa), homologous to the aeromonas caviae (r)-specific enoyl-coa hydratase gene (phaj(ac)) were cloned using a pcr technique to investigate the monomer-supplying ability for polyhydroxyalkanoate (pha) synthesis from beta-oxidation cycle. two expression plasmids for phaj1(pa) and phaj2(pa) were constructed and introduced into escherichia coli dh5alpha strain. the recombinants harboring phaj1(pa) or phaj2(pa) showed high (r)-specific enoyl ... | 2000 | 10713420 |
| inhibition of the aminopeptidase from aeromonas proteolytica by l-leucinethiol: kinetic and spectroscopic characterization of a slow, tight-binding inhibitor-enzyme complex. | the peptide inhibitor l-leucinethiol (leush) was found to be a potent, slow-binding inhibitor of the aminopeptidase from aeromonas proteolytica (aap). the overall potency (k(i)*) of leush was 7 nm while the corresponding alcohol l-leucinol (leuoh) was a simple competitive inhibitor of much lower potency (k(i) = 17 microm). these data suggest that the free thiol is likely involved in the formation of the e x i and e x i* complexes, presumably providing a metal ligand. in order to probe the nature ... | 2000 | 10714704 |
| genetic diversity of the fish pathogen aeromonas salmonicida demonstrated by random amplified polymorphic dna and pulsed-field gel electrophoresis analyses. | the current taxonomy of aeromonas salmonicida includes 4 subspecies. a. salmonicida subsp. salmonicida is associated with salmonid furunculosis, and a. salmonicida subsp. achromogenes, a. salmonicida subsp. masoucida, and a. salmonicida subsp. smithia are strains that show variation in some biochemical properties. this classification does not readily encompass isolates from a wide range of fish hosts currently described as atypical a. salmonicida. this study examined 17 typical strains, 39 atypi ... | 2000 | 10715816 |
| innate immunity and the normal microflora. | this paper discusses the following ten subtitles with the contents indicated. 1. to meet a microbe: discusses the four alternatives in host-microbe interactions. 2. receptors and signal transduction giving gene activation: discusses the lipopolysaccharide receptor and the limitations of cell cultures versus use of live animals. 3. effector molecules--antimicrobial peptides with and without cysteines. a data base exists with over 500 sequences. this paper gives a general overview of five classes ... | 2000 | 10719663 |
| characterization of vim-2, a carbapenem-hydrolyzing metallo-beta-lactamase and its plasmid- and integron-borne gene from a pseudomonas aeruginosa clinical isolate in france. | pseudomonas aeruginosa col-1 was identified in a blood culture of a 39-year-old-woman treated with imipenem in marseilles, france, in 1996. this strain was resistant to beta-lactams, including ureidopenicillins, ticarcillin-clavulanic acid, cefepime, ceftazidime, imipenem, and meropenem, but remained susceptible to the monobactam aztreonam. the carbapenem-hydrolyzing beta-lactamase gene of p. aeruginosa col-1 was cloned, sequenced, and expressed in escherichia coli dh10b. the deduced 266-amino-a ... | 2000 | 10722487 |
| cloning, sequencing, and role in serum susceptibility of porin ii from mesophilic aeromonas hydrophila. | we cloned and sequenced the structural gene for aeromonas hydrophila porin ii from strain ah-3 (serogroup o:34). the genetic position of this gene, like that of ompf in escherichia coli, is adjacent to aspc and transcribed in the same direction. however, upstream of the porin ii gene no similarities with e. coli were found. we obtained defined insertion mutants in porin ii gene either in a. hydrophila (o:34) or a. veronii sobria (serogroup o:11) serum-resistant or -sensitive strains. furthermore ... | 2000 | 10722573 |
| growth of aeromonas species on increasing concentrations of sodium chloride. | the growth of 16 strains of aeromonas, representing 12 species of the genera, were examined at different salt levels (0-1.71 m nacl). all the strains grew on media with 0.34 m nacl, and nine on media with 0.68 m. two strains, aer. enteropelogenes and aer. trota, were able to grow on media with 0.85 m and 1.02 m nacl, respectively. comparison of the growth curves of aer. hydrophila atcc7966 and aer. trota atcc 49657 on four concentrations of nacl (0.08, 0.34, 0.68 and 1.02 m) confirm the high tol ... | 2000 | 10728562 |
| successful use of the medicinal leech (hirudo medicinalis) for the treatment of venous insufficiency in a replanted digit. | | 1999 | 10731346 |
| quantitative determination of total coliforms and escherichia coli in marine waters with chromogenic and fluorogenic media. | this study compared the performance of lmx(r) broth (lmx), chromocult coliform(r) agar (cc) and chromocult coliform agar plus cefsulodin (10 microg ml-1) (cc-cfs), with standard methods multiple tube fermentation (mtf), for the enumeration of total coliforms and escherichia coli from marine recreational waters. lmx and cc are two media designed to concurrently detect total coliform (tc) bacteria and e. coli by the specific action of beta-galactosidase (total coliforms) and beta-glucuronidase (e. ... | 2000 | 10735996 |
| purification and some properties of high-molecular-weight xylanases, the xylanases 4 and 5 of aeromonas caviae w-61. | aeromonas caviae w-61 produces multiple extracellular xylanases, the xylanases 1, 2, 3, 4, and 5 [nguyen, v. d. et al., biosci. biotechnol. biochem., 56, 1708-1712 (1993)]. here we purified and characterized high-molecular-weight xylanases, the xylanases 4 and 5 from the culture fluids of the bacterium. the purified xylanases 4 and 5, which had molecular masses of 120 and 140 kda, respectively, were endo-beta-1,4-xylanases with similar enzymatic properties except for trans-xylosidase activity. t ... | 2000 | 10737201 |
| [isolation of aeromonas species from patients with sporadic diarrhea and characterization of aeromonas hydrophila isolates]. | a total of 16 strains of aeromonas species were isolated from feces of 348 patients with sporadic diarrhea in western kanagawa, japan from 1996 to 1998. of the 16 isolates, 7 were aeromonas hydrophila, 1 was a. sobria and 8 were a. caviae. the strains of a. hydrophila were examined for hemolytic activities, hemolysin gene types and o-serogroups. although all 7 strains of a. hydrophila showed hemolytic activities on sheep blood agar, in the test for hemolytic activities in culture supernatant, on ... | 2000 | 10741007 |
| identification of motile aeromonas strains with the microscan walkaway system in conjunction with the combo negative type 1s panels. | this study was performed to compare the microscan walkaway automated identification system in conjunction with the new microscan combo negative type 1s panels with conventional biochemical methods for identifying 85 environmental, clinical, and reference strains of eight aeromonas species. | 2000 | 10742279 |
| overproduction of the secretin outd suppresses the secretion defect of an erwinia chrysanthemi outb mutant. | outb is a component of the erwinia chrysanthemi out secretion machinery. homologues of outb have been described in two other bacteria, klebsiella oxytoca and aeromonas hydrophila, but their requirement in the secretion process seems to be different. study of outb topology with the blam topology probe suggests that it is an inner-membrane protein with a large periplasmic domain. however, fractionation experiments indicate that it could be associated with the outer membrane through its c-terminal ... | 2000 | 10746767 |
| adventures of a pore-forming toxin at the target cell surface. | the past three years have shed light on how the pore-forming toxin aerolysin binds to its target cell and then hijacks cellular devices to promote its own polymerization and pore formation. this selective permeabilization of the plasma membrane has unexpected intracellular consequences that might explain the importance of aerolysin in aeromonas pathogenicity. | 2000 | 10754575 |
| use of 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside for the isolation of beta-galactosidase-positive bacteria from municipal water supplies. | a new medium, mx-gal, has been developed for the membrane filter enumeration of beta-galactosidase-positive bacteria in municipal water supplies. mx-gal medium contains the chromogenic beta-galactosidase substrate 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside (x-gal). all aeromonas, citrobacter, and enterobacter strains isolated from raw water on mx-gal medium were beta-galactosidase positive. in contrast, only 10 to 20% of these strains produced a red colony with a metallic sheen on m-end ... | 1993 | 10755843 |
| aeromonas infection in acute suppurative cholangitis: review of 30 cases. | aeromonads, though not common pathogens in biliary sepsis, caused substantial mortality in patients with impaired hepatobiliary function. our aim was to study the pathogenic role of aeromonas in acute suppurative cholangitis. | 2000 | 10762115 |
| interaction of cefotetan and the metallo-beta-lactamases produced in aeromonas spp. and in vitro activity. | aeromonas spp. are increasingly being recognized as human pathogens. the presence of metallo-beta-lactamases in these organisms represents a potential problem in antimicrobial therapy. mechanism-based inactivators of beta-lactamases are used to overcome the resistance of clinical pathogens to beta-lactam antibiotics, but no clinical useful inhibitors of the metallo-beta-lactamases are presently known. studying the interaction between cefotetan and aeromonas spp. producing metallo-beta-lactamase ... | 2000 | 10765032 |
| amonabactin-mediated iron acquisition from transferrin and lactoferrin by aeromonas hydrophila: direct measurement of individual microscopic rate constants. | the effectiveness and mechanism of iron acquisition from transferrin or lactoferrin by aeromonas hydrophila has been analyzed with regard to the pathogenesis of this microbe. the ability of a. hydrophila's siderophore, amonabactin, to remove iron from transferrin was evaluated with in vitro competition experiments. the kinetics of iron removal from the three molecular forms of ferric transferrin (diferric, n- and c-terminal monoferric) were investigated by separating each form by urea gel electr ... | 2000 | 10766437 |
| the cytotoxic enterotoxin of aeromonas hydrophila induces proinflammatory cytokine production and activates arachidonic acid metabolism in macrophages. | an aerolysin-related cytotoxic enterotoxin (act) of aeromonas hydrophila possesses multiple biological activities, which include its ability to lyse red blood cells, destroy tissue culture cell lines, evoke a fluid secretory response in ligated intestinal loop models, and induce lethality in mice. the role of act in the virulence of the organism has been demonstrated. in this study, we evaluated the potential of act to induce production of proinflammatory cytokines associated with act-induced ti ... | 2000 | 10768977 |
| characterization of the metallo-beta-lactamase determinant of acinetobacter baumannii ac-54/97 reveals the existence of bla(imp) allelic variants carried by gene cassettes of different phylogeny. | the metallo-beta-lactamase determinant of acinetobacter baumannii ac-54/97, a clinical isolate from italy that was previously shown to produce an enzyme related to imp-1, was isolated by means of a pcr methodology which targets amplification of gene cassette arrays inserted into class 1 integrons. sequencing revealed that this determinant was an allelic variant (named bla(imp-2)) of bla(imp) found in japanese isolates and that it was divergent from the latter by 12% of its nucleotide sequence, w ... | 2000 | 10770756 |